Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0014359

Physical Location & Seq

Genomic Coordinates Forward (+)

18661853 .. 18663979

Gene Structure 2127 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g16280.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGAGCTTCCCTCCATCGACTGCATCTGGCGCAATGAAAGTGTTTCGCGGAAAGCTACATTTTCCAATTATCATGGTCTGCGCTATCGCCTTCATCAGTCTCCTCTACACCGAACGGATTGGCATCATTCTTTCCTCCAACCCTTTTTTCAAAGTTAAATCATGTTCCAAAACCAATGCCACTTCGAAAAATAAAAATAGAAATGCTACACTGGATAAGTTGAGGGATACCATATTAGATGATAGGTTTGAGTTTGACCCTGAGGAGTGCAGCATTGAAAATGGAAAGTGGGTGTTCAACCGTTCGATCGAGCCTCTTTACTCGGATAGAAGTTGCCCGTATCTTGATAGGCAAGTTTCGTGTGTCAAGAATGGACGTCCGGATTCTGGCTATCGCTACTGGCAATGGCAGATCCAAGATTGCACATTGCCAAGGCATACGCAATCTTCATGGATTAATTTAAATACGTACTGCATGAAGTTCTGTTTTTCTAAACAGTTAATTTCTTACAACTTTGTATGTAGATTTGATCCAGAAGTTGTTCTTAAGAAACTTAGCGGAAAGAGGCTACTTTTTGTTGGGGATTCTTTGCAAAGAGGTCAATGGCAATCTCTAGTCTGTATGGTTGAATCCATCATACCAGAGGACCAAAAGTCCATAAGACGCGGGCACTCTCACACAGTCTTCAGTGCCAAGGAGTATAATGCAAGTATTGAATTCTACTGGGCTCCATTTCTCGTTGATTCCAATTCTGATGAACAGATAATCGGAAATCCGAAGGAAAGAATACTAAAAGTGGATTCAGTTGCCAAGCATGCCAAATACTGGACAGGAGTTGATATCCTTGTGTTCAACACTTACGTTTGGTGGATGAGCGGCTACACGATCAAATCATACTGGGGCTCGTTTCCAAACGGAGAAGAAGGGTACGAAGAATTGGAAGCACCGGCTGCCTACAGAATAGCATTGAAGACATGGGCTAACTGGGTCGACACAAATGTGAATCCGAACAAGACCCGTGTTTTTTTCACTACTATGTCCCCTACACACATGAGAAGCGCAGACTGGGAAAATCCAGATGGGATGAGATGCTTCAACGAAACAAAGCCATACATGAAAAAGGGATTCTGGGGAACTGGTTCGGACAAGAAGATCATGCGTGTGGTGTCCGAAGTAATAAACAAAATGAAATTTCCGGTTTCAGTACTCAATGTAACGCAGATGTCAAATTACAGAGTTGATGCTCACACAAAAGTTTACACCGAAACCGGAGGCCAAGTACTAACTGATGAGCAAAAGGCTGATGTGCTGCACAATTCAGATTGCATACATTGGTGCCTTCCTGGAGTTCCGGACACATGGAATCAAATTTTTGTGGCACATTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009250 GO:0009664 GO:0009827 GO:0009987 GO:0010383 GO:0010393 GO:0010410 GO:0012505 GO:0016020 GO:0016043 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0030243 GO:0030244 GO:0033692 GO:0034637 GO:0034645 GO:0042545 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045488 GO:0045489 GO:0045491 GO:0045492 GO:0051273 GO:0051274 GO:0070589 GO:0070592 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

463

Amino Acids

53.38

Weight (kDa)

8.72

Isoelectric Point (pI)

35.24

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01360 AT5G01360
fragaria_vesca	FvH4_6g11230 FvH4_6g11230
malus_domestica	MD00G1172500.v1.1 MD12G1171500.v1.1
prunus_persica	Prupe.6G279700_v2.0.a1
pyrus_communis	pycom04g14210 pycom12g16280
rosa_chinensis	RchiOBHm_Chr3g0462741
rosa_laevigata	RLG00000024836
rosa_multiflora	Rmu_sc0000766.1_g000003 Rmu_sc0011057.1_g000007
rosa_roxburghii	Rroxscaffold_6G00417240
rosa_rugosa	Rorug03G0058900
rosa_samantha	Rh3DG122300
rosa_wichuraiana	Rw3G009900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	379
AccB1I	GGYRCC	1 cut(s)	1335
AccB7I	CCANNNNNTGG	1 cut(s)	825
AccBSI	CCGCTC	1 cut(s)	876
AccI	GTMKAC	1 cut(s)	990
AccII	CGCG	2 cut(s)	48, 666
AccIII	TCCGGA	2 cut(s)	379, 1351
AciI	CCGC	4 cut(s)	48, 558, 666, 876
AclWI	GGATC	2 cut(s)	406, 524
AcsI	RAATTY	3 cut(s)	716, 1190, 1368
AcuI	CTGAAG	1 cut(s)	670
AcyI	GRCGYC	1 cut(s)	376
AfaI	GTAC	4 cut(s)	470, 929, 1206, 1281
AfiI	CCNNNNNNNGG	2 cut(s)	386, 825
AflII	CTTAAG	1 cut(s)	545
AgsI	TTSAA	8 cut(s)	151, 278, 298, 629, 716, 853, 970, 1096
AhdI	GACNNNNNGTC	1 cut(s)	652
AjnI	CCWGG	1 cut(s)	1342
AjuI	GAANNNNNNNTTGG	2 cut(s)	131, 163
AleI	CACNNNNGTG	1 cut(s)	998
AluBI	AGCT	2 cut(s)	6, 55
AluI	AGCT	2 cut(s)	6, 55
Alw26I	GTCTC	1 cut(s)	104
AlwI	GGATC	2 cut(s)	406, 524
Aor13HI	TCCGGA	2 cut(s)	379, 1351
AoxI	GGCC	1 cut(s)	1273
ApeKI	GCWGC	3 cut(s)	270, 950, 1309
ApoI	RAATTY	3 cut(s)	716, 1190, 1368
AseI	ATTAAT	1 cut(s)	456
Asp700I	GAANNNNTTC	2 cut(s)	540, 1138
AspLEI	GCGC	3 cut(s)	32, 83, 1061
AspS9I	GGNCC	1 cut(s)	646
AsuII	TTCGAA	1 cut(s)	185
AvaII	GGWCC	1 cut(s)	646
AxyI	CCTNAGG	1 cut(s)	261
BaeGI	GKGCMC	1 cut(s)	672
BanI	GGYRCC	1 cut(s)	1335
BanII	GRGCYC	2 cut(s)	730, 905
BbsI	GAAGAC	2 cut(s)	676, 977
BbvI	GCAGC	3 cut(s)	282, 937, 1296
BccI	CCATC	3 cut(s)	22, 641, 1073
BciT130I	CCWGG	1 cut(s)	1344
BciVI	GTATCC	1 cut(s)	220
BcoDI	GTCTC	1 cut(s)	104
BfaI	CTAG	1 cut(s)	614
BfmI	CTRYAG	1 cut(s)	955
BfrI	CTTAAG	1 cut(s)	545
BfuI	GTATCC	1 cut(s)	220
BisI	GCNGC	4 cut(s)	271, 877, 951, 1310
BlsI	GCNGC	4 cut(s)	272, 878, 952, 1311
BmcAI	AGTACT	2 cut(s)	1206, 1281
Bme1390I	CCNGG	1 cut(s)	1344
Bme18I	GGWCC	1 cut(s)	646
BmeRI	GACNNNNNGTC	1 cut(s)	652
BmgT120I	GGNCC	1 cut(s)	646
BmiI	GGNNCC	2 cut(s)	729, 1337
BmrFI	CCNGG	1 cut(s)	1344
BmrI	ACTGGG	4 cut(s)	733, 907, 994, 1075
BmsI	GCATC	4 cut(s)	32, 132, 1079, 1231
BmuI	ACTGGG	4 cut(s)	733, 907, 994, 1075
BpiI	GAAGAC	2 cut(s)	676, 977
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	1365
Bpu14I	TTCGAA	1 cut(s)	185
BsaAI	YACGTR	1 cut(s)	468
BsaBI	GATNNNNATC	1 cut(s)	122
BsaHI	GRCGYC	1 cut(s)	376
BsaJI	CCNNGG	2 cut(s)	431, 693
BsaWI	WCCGGW	4 cut(s)	379, 1195, 1268, 1351
BsaXI	ACNNNNNCTCC	2 cut(s)	1263, 1293
Bsc4I	CCNNNNNNNGG	2 cut(s)	386, 825
Bse118I	RCCGGY	1 cut(s)	946
Bse1I	ACTGG	8 cut(s)	216, 404, 728, 830, 902, 989, 1070, 1141
Bse21I	CCTNAGG	1 cut(s)	261
Bse3DI	GCAATG	3 cut(s)	39, 410, 425
Bse8I	GATNNNNATC	1 cut(s)	122
BseAI	TCCGGA	2 cut(s)	379, 1351
BseBI	CCWGG	1 cut(s)	1344
BseDI	CCNNGG	2 cut(s)	431, 693
BseGI	GGATG	2 cut(s)	876, 1089
BseJI	GATNNNNATC	1 cut(s)	122
BseLI	CCNNNNNNNGG	2 cut(s)	386, 825
BseMI	GCAATG	3 cut(s)	39, 410, 425
BseMII	CTCAG	1 cut(s)	252
BseNI	ACTGG	8 cut(s)	216, 404, 728, 830, 902, 989, 1070, 1141
BseRI	GAGGAG	2 cut(s)	92, 278
BseSI	GKGCMC	1 cut(s)	672
BseXI	GCAGC	3 cut(s)	282, 937, 1296
BsgI	GTGCAG	2 cut(s)	289, 1295
Bsh1236I	CGCG	2 cut(s)	48, 666
Bsh1285I	CGRYCG	1 cut(s)	309
BshFI	GGCC	1 cut(s)	1275
BshNI	GGYRCC	1 cut(s)	1335
BsiEI	CGRYCG	1 cut(s)	309
BsiSI	CCGG	5 cut(s)	380, 947, 1196, 1269, 1352
BslFI	GGGAC	1 cut(s)	1024
BslI	CCNNNNNNNGG	2 cut(s)	386, 825
BsmAI	GTCTC	1 cut(s)	104
BsmFI	GGGAC	1 cut(s)	1024
BsnI	GGCC	1 cut(s)	1275
Bsp119I	TTCGAA	1 cut(s)	185
Bsp1286I	GDGCHC	3 cut(s)	672, 730, 905
Bsp13I	TCCGGA	2 cut(s)	379, 1351
Bsp143I	GATC	5 cut(s)	306, 411, 529, 885, 1152
BspACI	CCGC	4 cut(s)	48, 558, 666, 876
BspANI	GGCC	1 cut(s)	1275
BspCNI	CTCAG	1 cut(s)	253
BspEI	TCCGGA	2 cut(s)	379, 1351
BspFNI	CGCG	2 cut(s)	48, 666
BspLI	GGNNCC	2 cut(s)	729, 1337
BspPI	GGATC	2 cut(s)	406, 524
BspT104I	TTCGAA	1 cut(s)	185
BspT107I	GGYRCC	1 cut(s)	1335
BspTI	CTTAAG	1 cut(s)	545
BsrBI	CCGCTC	1 cut(s)	876
BsrDI	GCAATG	3 cut(s)	39, 410, 425
BsrFI	RCCGGY	1 cut(s)	946
BsrI	ACTGG	8 cut(s)	216, 404, 728, 830, 902, 989, 1070, 1141
BssAI	RCCGGY	1 cut(s)	946
BssECI	CCNNGG	2 cut(s)	431, 693
BssMI	GATC	5 cut(s)	306, 411, 529, 885, 1152
BssNI	GRCGYC	1 cut(s)	376
BssT1I	CCWWGG	2 cut(s)	431, 693
Bst2UI	CCWGG	1 cut(s)	1344
Bst4CI	ACNGT	3 cut(s)	302, 498, 682
BstACI	GRCGYC	1 cut(s)	376
BstAFI	CTTAAG	1 cut(s)	545
BstAPI	GCANNNNNTGC	1 cut(s)	950
BstBAI	YACGTR	1 cut(s)	468
BstBI	TTCGAA	1 cut(s)	185
BstC8I	GCNNGC	2 cut(s)	668, 816
BstDEI	CTNAG	2 cut(s)	261, 554
BstF5I	GGATG	2 cut(s)	876, 1089
BstFNI	CGCG	2 cut(s)	48, 666
BstHHI	GCGC	3 cut(s)	32, 83, 1061
BstKTI	GATC	5 cut(s)	309, 414, 532, 888, 1155
BstMAI	GTCTC	1 cut(s)	104
BstMBI	GATC	5 cut(s)	306, 411, 529, 885, 1152
BstMCI	CGRYCG	1 cut(s)	309
BstMWI	GCNNNNNNNGC	3 cut(s)	29, 815, 950
BstNI	CCWGG	1 cut(s)	1344
BstNSI	RCATGY	1 cut(s)	818
BstSCI	CCNGG	1 cut(s)	1342
BstSFI	CTRYAG	1 cut(s)	955
BstSLI	GKGCMC	1 cut(s)	672
BstSNI	TACGTA	1 cut(s)	468
BstUI	CGCG	2 cut(s)	48, 666
BstV1I	GCAGC	3 cut(s)	282, 937, 1296
BstV2I	GAAGAC	2 cut(s)	676, 977
BstX2I	RGATCY	1 cut(s)	411
BstYI	RGATCY	1 cut(s)	411
Bsu36I	CCTNAGG	1 cut(s)	261
BsuI	GTATCC	1 cut(s)	220
BsuRI	GGCC	1 cut(s)	1275
BtsCI	GGATG	2 cut(s)	876, 1089
BtsIMutI	CAGTG	2 cut(s)	209, 694
Cac8I	GCNNGC	2 cut(s)	668, 816
CfoI	GCGC	3 cut(s)	32, 83, 1061
Cfr10I	RCCGGY	1 cut(s)	946
Cfr13I	GGNCC	1 cut(s)	646
CseI	GACGC	1 cut(s)	672
Csp6I	GTAC	4 cut(s)	469, 928, 1205, 1280
CviQI	GTAC	4 cut(s)	469, 928, 1205, 1280
DdeI	CTNAG	2 cut(s)	261, 554
DpnI	GATC	5 cut(s)	308, 413, 531, 887, 1154
DpnII	GATC	5 cut(s)	306, 411, 529, 885, 1152
DraI	TTTAAA	1 cut(s)	462
DriI	GACNNNNNGTC	1 cut(s)	652
Eam1105I	GACNNNNNGTC	1 cut(s)	652
Eco105I	TACGTA	1 cut(s)	468
Eco130I	CCWWGG	2 cut(s)	431, 693
Eco24I	GRGCYC	2 cut(s)	730, 905
Eco32I	GATATC	1 cut(s)	841
Eco47I	GGWCC	1 cut(s)	646
Eco57I	CTGAAG	1 cut(s)	670
Eco81I	CCTNAGG	1 cut(s)	261
EcoRI	GAATTC	1 cut(s)	716
EcoRII	CCWGG	1 cut(s)	1342
EcoRV	GATATC	1 cut(s)	841
EcoT14I	CCWWGG	2 cut(s)	431, 693
EcoT38I	GRGCYC	2 cut(s)	730, 905
ErhI	CCWWGG	2 cut(s)	431, 693
FalI	AAGNNNNNCTT	2 cut(s)	528, 560
FaqI	GGGAC	1 cut(s)	1024
FauI	CCCGC	1 cut(s)	659
FblI	GTMKAC	1 cut(s)	990
Fnu4HI	GCNGC	4 cut(s)	271, 877, 951, 1310
FokI	GGATG	2 cut(s)	883, 1096
FriOI	GRGCYC	2 cut(s)	730, 905
Fsp4HI	GCNGC	4 cut(s)	271, 877, 951, 1310
FspBI	CTAG	1 cut(s)	614
GlaI	GCGC	3 cut(s)	31, 82, 1060
GluI	GCNGC	4 cut(s)	271, 877, 951, 1310
GsuI	CTGGAG	1 cut(s)	1365
HaeIII	GGCC	1 cut(s)	1275
HapII	CCGG	5 cut(s)	380, 947, 1196, 1269, 1352
HgaI	GACGC	1 cut(s)	672
HhaI	GCGC	3 cut(s)	32, 83, 1061
Hin1I	GRCGYC	1 cut(s)	376
Hin6I	GCGC	3 cut(s)	30, 81, 1059
HinP1I	GCGC	3 cut(s)	30, 81, 1059
HincII	GTYRAC	1 cut(s)	991
HindII	GTYRAC	1 cut(s)	991
HinfI	GANTC	8 cut(s)	383, 584, 629, 743, 800, 1003, 1125, 1363
HpaII	CCGG	5 cut(s)	380, 947, 1196, 1269, 1352
Hpy166II	GTNNAC	2 cut(s)	991, 1258
Hpy188I	TCNGA	8 cut(s)	325, 754, 770, 777, 1008, 1144, 1171, 1321
Hpy188III	TCNNGA	6 cut(s)	344, 367, 380, 533, 1076, 1352
Hpy8I	GTNNAC	2 cut(s)	991, 1258
HpyAV	CCTTC	4 cut(s)	100, 772, 917, 1349
HpyCH4III	ACNGT	3 cut(s)	302, 498, 682
HpyCH4IV	ACGT	3 cut(s)	376, 467, 861
HpyCH4V	TGCA	8 cut(s)	23, 270, 423, 474, 592, 707, 1312, 1326
HpyF10VI	GCNNNNNNNGC	3 cut(s)	29, 815, 950
HpyF3I	CTNAG	2 cut(s)	261, 554
HpySE526I	ACGT	3 cut(s)	376, 467, 861
Hsp92I	GRCGYC	1 cut(s)	376
HspAI	GCGC	3 cut(s)	30, 81, 1059
Kpn2I	TCCGGA	2 cut(s)	379, 1351
Kzo9I	GATC	5 cut(s)	306, 411, 529, 885, 1152
LmnI	GCTCC	1 cut(s)	733
Lsp1109I	GCAGC	3 cut(s)	282, 937, 1296
LweI	GCATC	4 cut(s)	32, 132, 1079, 1231
MaeI	CTAG	1 cut(s)	614
MaeII	ACGT	3 cut(s)	376, 467, 861
MaeIII	GTNAC	1 cut(s)	1213
MalI	GATC	5 cut(s)	308, 413, 531, 887, 1154
MbiI	CCGCTC	1 cut(s)	876
MboI	GATC	5 cut(s)	306, 411, 529, 885, 1152
MboII	GAAGA	6 cut(s)	438, 676, 932, 944, 982, 1162
MflI	RGATCY	1 cut(s)	411
MhlI	GDGCHC	3 cut(s)	672, 730, 905
MmeI	TCCRAC	1 cut(s)	162
MroI	TCCGGA	2 cut(s)	379, 1351
MroXI	GAANNNNTTC	2 cut(s)	540, 1138
MseI	TTAA	5 cut(s)	156, 456, 461, 500, 546
MslI	CAYNNNNRTG	3 cut(s)	998, 1034, 1160
MspCI	CTTAAG	1 cut(s)	545
MspI	CCGG	5 cut(s)	380, 947, 1196, 1269, 1352
MspR9I	CCNGG	1 cut(s)	1344
MvaI	CCWGG	1 cut(s)	1344
MvnI	CGCG	2 cut(s)	48, 666
MwoI	GCNNNNNNNGC	3 cut(s)	29, 815, 950
NdeII	GATC	5 cut(s)	306, 411, 529, 885, 1152
NlaIV	GGNNCC	2 cut(s)	729, 1337
NspI	RCATGY	1 cut(s)	818
NspV	TTCGAA	1 cut(s)	185
OliI	CACNNNNGTG	1 cut(s)	998
PaeI	GCATGC	1 cut(s)	818
PdmI	GAANNNNTTC	2 cut(s)	540, 1138
PfeI	GAWTC	8 cut(s)	383, 584, 629, 743, 800, 1003, 1125, 1363
PflMI	CCANNNNNTGG	1 cut(s)	825
PfoI	TCCNGGA	1 cut(s)	1342
PkrI	GCNGC	4 cut(s)	272, 878, 952, 1311
Ple19I	CGATCG	1 cut(s)	309
Ppu21I	YACGTR	1 cut(s)	468
PshBI	ATTAAT	1 cut(s)	456
Psp6I	CCWGG	1 cut(s)	1342
PspGI	CCWGG	1 cut(s)	1342
PspN4I	GGNNCC	2 cut(s)	729, 1337
PspPI	GGNCC	1 cut(s)	646
PsuI	RGATCY	1 cut(s)	411
PvuI	CGATCG	1 cut(s)	309
RsaI	GTAC	4 cut(s)	470, 929, 1206, 1281
RsaNI	GTAC	4 cut(s)	469, 928, 1205, 1280
RseI	CAYNNNNRTG	3 cut(s)	998, 1034, 1160
SalI	GTCGAC	1 cut(s)	989
SaqAI	TTAA	5 cut(s)	156, 456, 461, 500, 546
SatI	GCNGC	4 cut(s)	271, 877, 951, 1310
Sau3AI	GATC	5 cut(s)	306, 411, 529, 885, 1152
Sau96I	GGNCC	1 cut(s)	646
ScaI	AGTACT	2 cut(s)	1206, 1281
ScrFI	CCNGG	1 cut(s)	1344
SduI	GDGCHC	3 cut(s)	672, 730, 905
SetI	ASST	7 cut(s)	8, 57, 248, 379, 470, 601, 864
SfaNI	GCATC	4 cut(s)	32, 132, 1079, 1231
SfcI	CTRYAG	1 cut(s)	955
SfuI	TTCGAA	1 cut(s)	185
SinI	GGWCC	1 cut(s)	646
SmiI	ATTTAAAT	1 cut(s)	462
SmiMI	CAYNNNNRTG	3 cut(s)	998, 1034, 1160
SmlI	CTYRAG	1 cut(s)	545
SmoI	CTYRAG	1 cut(s)	545
SnaBI	TACGTA	1 cut(s)	468
SphI	GCATGC	1 cut(s)	818
SsiI	CCGC	4 cut(s)	48, 558, 666, 876
SspMI	CTAG	1 cut(s)	614
StyD4I	CCNGG	1 cut(s)	1342
StyI	CCWWGG	2 cut(s)	431, 693
SwaI	ATTTAAAT	1 cut(s)	462
TaaI	ACNGT	3 cut(s)	302, 498, 682
TaiI	ACGT	3 cut(s)	379, 470, 864
TaqI	TCGA	5 cut(s)	17, 185, 305, 309, 990
TatI	WGTACW	2 cut(s)	1204, 1279
TauI	GCSGC	1 cut(s)	879
TfiI	GAWTC	8 cut(s)	383, 584, 629, 743, 800, 1003, 1125, 1363
Tru1I	TTAA	5 cut(s)	156, 456, 461, 500, 546
Tru9I	TTAA	5 cut(s)	156, 456, 461, 500, 546
TscAI	CASTG	2 cut(s)	216, 694
TseI	GCWGC	3 cut(s)	270, 950, 1309
TspDTI	ATGAA	7 cut(s)	50, 82, 438, 491, 771, 1130, 1202
TspGWI	ACGGA	2 cut(s)	130, 930
TspRI	CASTG	2 cut(s)	216, 694
Van91I	CCANNNNNTGG	1 cut(s)	825
Vha464I	CTTAAG	1 cut(s)	545
VpaK11BI	GGWCC	1 cut(s)	646
VspI	ATTAAT	1 cut(s)	456
XapI	RAATTY	3 cut(s)	716, 1190, 1368
XceI	RCATGY	1 cut(s)	818
XmiI	GTMKAC	1 cut(s)	990
XmnI	GAANNNNTTC	2 cut(s)	540, 1138
XspI	CTAG	1 cut(s)	614
ZraI	GACGTC	1 cut(s)	377
ZrmI	AGTACT	2 cut(s)	1206, 1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.