MD00G1207400.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014810
Physical Location & Seq
Reverse (-)
50488264 .. 50489458
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1207400.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGATGCTTTTGCGGAGCGGTTGAAGTCCGATCTTGAAAAATTGAAGCAGAACAAGCTCGAGCTCAAGCCGTGTGATTATGATGACGATGTTGACGATGATACCGACTACAATGCTAAACGCGGTTCTCTTGATGCTTATGCTTATCATGATCTTCTCGTTTCTTTGGCAATAGATTGTTTGCTTACAGGGAACCCTTGGGAGGCACATAAGGGGCGCTCCATTTCGCTGGAAGAAAGCACTGCGAGGAGGCTTCTCACCCCCGAATCAGATGTATCGCGGGAGTGGGAGCGGCTGAGGAAGGAGGTTTTGGCGCCCTTGAAAAACTACTCTAGTCGTCAAAGTCGGTTTGGCAGGTGGGAGCGCTGCGAGGTGAAGAAGTACTTGGAGGAGGTGAAAGCAGCAACAACAATTATAAAGCCCGACGCTTTACTTCCATATGACATCCTACGCTATCTGGAAGATGGTAATGTTGGGGAAGCGGCAGAGCTTCAATGGAAGGCAATGGTGCAAAAGGTCAAGGAGGGCGGCAAATTCAAAAACTGCTGGGCTGTATGTGACAACGGCTGGTGCCAGTCATGGAGTTTGAGTTTGGGACTTTTGGTGTATGAACTGAGTGAAGAGCCATGGAACCGAAAAGTGATGAATTTCAGTCTGCCTGATTCCCGCTGTAAGTTTATTCCGCCCATTCATTTTTTGCCCCAGCTGCATTCCATTCAGGGCCTCGACGATGTTAAGTCCAAGTTCTCCTTTCTGACGAGAATGGAGAGGAGAATGGACATAAGCAGCATATGTATTGATATACAAATGGTGTTTGATGCGATCTTGGAAGTTGTTGTCAAGGAGAATTTGAAGCCAGAGCAGATGATCAAGAAGCTGTTTCTGTTCACCGACTGCGCAGGCTATGATTGGAAGGGTTACGAGAATCTGTATGAGGCCGTACGGAGCATGTTCGGGGACAAGGGGTATGGGGATGATGCGGTGCCACACATTGTGTTTTGGGATTTTTGGTGGGGGTATCGTTCTCCATCCTTTGATTTTCCTCATAGAGGGGTGACGGTGTTGCGTGGCTACTCCGAAAATTTGGTCAAATCCTTCCTGGACAATGGTGGGGATTTTCGCCCTCACCATCTCATGGAAGTAGCCATTGCCGACAAAGAATATCAAACTCTTGCTGTAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

396

Amino Acids

45.82

Weight (kDa)

5.32

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7788 PF25043 193 - 373 3.2e-29 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
AarI CACCTGC 1 cut(s) 345
Acc16I TGCGCA 1 cut(s) 898
Acc36I ACCTGC 1 cut(s) 345
AccB1I GGYRCC 3 cut(s) 313, 570, 982
AccB7I CCANNNNNTGG 1 cut(s) 1135
AccBSI CCGCTC 2 cut(s) 19, 292
AccI GTMKAC 1 cut(s) 1182
AccII CGCG 2 cut(s) 123, 280
AcsI RAATTY 4 cut(s) 533, 646, 847, 1081
AcyI GRCGYC 1 cut(s) 314
AdeI CACNNNGTG 1 cut(s) 994
AfaI GTAC 2 cut(s) 383, 942
AfeI AGCGCT 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 202, 1049, 1135
AgsI TTSAA 7 cut(s) 25, 38, 46, 322, 494, 538, 853
AjnI CCWGG 1 cut(s) 1098
AjuI GAANNNNNNNTTGG 4 cut(s) 293, 325, 368, 400
AluBI AGCT 5 cut(s) 58, 64, 490, 706, 877
AluI AGCT 5 cut(s) 58, 64, 490, 706, 877
Alw21I GWGCWC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 59
Aor51HI AGCGCT 1 cut(s) 365
AoxI GGCC 2 cut(s) 721, 936
ApeKI GCWGC 4 cut(s) 366, 401, 706, 786
ApoI RAATTY 4 cut(s) 533, 646, 847, 1081
ArsI GACNNNNNNTTYG 2 cut(s) 1071, 1103
AspLEI GCGC 4 cut(s) 219, 316, 366, 899
AspS9I GGNCC 1 cut(s) 721
AsuHPI GGTGA 6 cut(s) 250, 385, 406, 880, 1066, 1118
AvaI CYCGRG 1 cut(s) 59
BanI GGYRCC 3 cut(s) 313, 570, 982
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 4 cut(s) 353, 413, 693, 798
BccI CCATC 3 cut(s) 458, 1036, 1137
BceAI ACGGC 3 cut(s) 55, 580, 923
BcgI CGANNNNNNTGC 2 cut(s) 95, 129
BciT130I CCWGG 1 cut(s) 1100
BclI TGATCA 1 cut(s) 867
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 1176
BfoI RGCGCY 3 cut(s) 220, 317, 367
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 7 cut(s) 293, 367, 402, 483, 529, 707, 787
BlsI GCNGC 7 cut(s) 294, 368, 403, 484, 530, 708, 788
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 1 cut(s) 1100
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 721
BmiI GGNNCC 5 cut(s) 194, 315, 572, 632, 984
BmrFI CCNGG 1 cut(s) 1100
BmsI GCATC 3 cut(s) 124, 808, 967
Bpu10I CCTNAGC 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 197, 626
BsaXI ACNNNNNCTCC 2 cut(s) 574, 604
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 1049, 1135
Bse1I ACTGG 1 cut(s) 574
Bse3DI GCAATG 2 cut(s) 510, 1146
BseBI CCWGG 1 cut(s) 1100
BseDI CCNNGG 2 cut(s) 197, 626
BseGI GGATG 4 cut(s) 10, 444, 979, 1028
BseLI CCNNNNNNNGG 3 cut(s) 202, 1049, 1135
BseMI GCAATG 2 cut(s) 510, 1146
BseMII CTCAG 2 cut(s) 287, 605
BseNI ACTGG 1 cut(s) 574
BseRI GAGGAG 3 cut(s) 262, 404, 784
BseXI GCAGC 4 cut(s) 353, 413, 693, 798
BseYI CCCAGC 2 cut(s) 546, 702
Bsh1236I CGCG 2 cut(s) 123, 280
BshFI GGCC 2 cut(s) 723, 938
BshNI GGYRCC 3 cut(s) 313, 570, 982
BsiHKAI GWGCWC 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 59
BsiWI CGTACG 1 cut(s) 940
BslFI GGGAC 2 cut(s) 609, 971
BslI CCNNNNNNNGG 3 cut(s) 202, 1049, 1135
BsmFI GGGAC 2 cut(s) 609, 971
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 2 cut(s) 723, 938
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 4 cut(s) 31, 151, 822, 867
Bsp19I CCATGG 1 cut(s) 626
BspANI GGCC 2 cut(s) 723, 938
BspCNI CTCAG 2 cut(s) 288, 606
BspFNI CGCG 2 cut(s) 123, 280
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 5 cut(s) 194, 315, 572, 632, 984
BspMI ACCTGC 1 cut(s) 345
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 3 cut(s) 313, 570, 982
BsrBI CCGCTC 2 cut(s) 19, 292
BsrDI GCAATG 2 cut(s) 510, 1146
BsrI ACTGG 1 cut(s) 574
BssECI CCNNGG 2 cut(s) 197, 626
BssMI GATC 4 cut(s) 31, 151, 822, 867
BssNI GRCGYC 1 cut(s) 314
BssT1I CCWWGG 2 cut(s) 197, 626
Bst2UI CCWGG 1 cut(s) 1100
Bst4CI ACNGT 1 cut(s) 1060
Bst6I CTCTTC 1 cut(s) 615
BstACI GRCGYC 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 901
BstDEI CTNAG 2 cut(s) 296, 614
BstDSI CCRYGG 1 cut(s) 626
BstENI CCTNNNNNAGG 1 cut(s) 1047
BstF5I GGATG 4 cut(s) 10, 444, 979, 1028
BstFNI CGCG 2 cut(s) 123, 280
BstH2I RGCGCY 3 cut(s) 220, 317, 367
BstHHI GCGC 4 cut(s) 219, 316, 366, 899
BstKTI GATC 4 cut(s) 34, 154, 825, 870
BstMBI GATC 4 cut(s) 31, 151, 822, 867
BstMWI GCNNNNNNNGC 2 cut(s) 55, 706
BstNI CCWGG 1 cut(s) 1100
BstNSI RCATGY 1 cut(s) 952
BstSCI CCNGG 1 cut(s) 1098
BstSFI CTRYAG 1 cut(s) 1176
BstUI CGCG 2 cut(s) 123, 280
BstV1I GCAGC 4 cut(s) 353, 413, 693, 798
BstXI CCANNNNNNTGG 1 cut(s) 229
BsuRI GGCC 2 cut(s) 723, 938
BtgI CCRYGG 1 cut(s) 626
BtsCI GGATG 4 cut(s) 10, 444, 979, 1028
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 1 cut(s) 901
CciI TCATGA 1 cut(s) 148
CfoI GCGC 4 cut(s) 219, 316, 366, 899
Cfr13I GGNCC 1 cut(s) 721
CseI GACGC 1 cut(s) 434
Csp6I GTAC 2 cut(s) 382, 941
CviAII CATG 5 cut(s) 149, 579, 627, 949, 1135
CviQI GTAC 2 cut(s) 382, 941
DdeI CTNAG 2 cut(s) 296, 614
DinI GGCGCC 1 cut(s) 315
DpnI GATC 4 cut(s) 33, 153, 824, 869
DpnII GATC 4 cut(s) 31, 151, 822, 867
DraIII CACNNNGTG 1 cut(s) 994
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
EciI GGCGGA 1 cut(s) 672
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 2 cut(s) 197, 626
Eco24I GRGCYC 1 cut(s) 66
Eco47III AGCGCT 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 59
EcoICRI GAGCTC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 1047
EcoO109I RGGNCCY 1 cut(s) 721
EcoRII CCWGG 1 cut(s) 1098
EcoT14I CCWWGG 2 cut(s) 197, 626
EcoT38I GRGCYC 1 cut(s) 66
EgeI GGCGCC 1 cut(s) 315
EheI GGCGCC 1 cut(s) 315
ErhI CCWWGG 2 cut(s) 197, 626
FaeI CATG 5 cut(s) 152, 582, 630, 952, 1138
FalI AAGNNNNNCTT 4 cut(s) 368, 400, 734, 766
FaqI GGGAC 2 cut(s) 609, 971
FatI CATG 5 cut(s) 148, 578, 626, 948, 1134
FauI CCCGC 2 cut(s) 273, 674
FauNDI CATATG 2 cut(s) 439, 791
FbaI TGATCA 1 cut(s) 867
FblI GTMKAC 1 cut(s) 1182
Fnu4HI GCNGC 7 cut(s) 293, 367, 402, 483, 529, 707, 787
FokI GGATG 4 cut(s) 17, 431, 986, 1015
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 7 cut(s) 293, 367, 402, 483, 529, 707, 787
FspBI CTAG 1 cut(s) 333
FspI TGCGCA 1 cut(s) 898
GlaI GCGC 4 cut(s) 218, 315, 365, 898
GluI GCNGC 7 cut(s) 293, 367, 402, 483, 529, 707, 787
GsaI CCCAGC 2 cut(s) 550, 706
HaeII RGCGCY 3 cut(s) 220, 317, 367
HaeIII GGCC 2 cut(s) 723, 938
HgaI GACGC 1 cut(s) 434
HhaI GCGC 4 cut(s) 219, 316, 366, 899
Hin1I GRCGYC 1 cut(s) 314
Hin1II CATG 5 cut(s) 152, 582, 630, 952, 1138
Hin6I GCGC 4 cut(s) 217, 314, 364, 897
HinP1I GCGC 4 cut(s) 217, 314, 364, 897
HincII GTYRAC 2 cut(s) 94, 1183
HindII GTYRAC 2 cut(s) 94, 1183
HinfI GANTC 3 cut(s) 266, 662, 925
HphI GGTGA 6 cut(s) 250, 385, 406, 880, 1066, 1118
Hpy166II GTNNAC 3 cut(s) 94, 888, 1183
Hpy188I TCNGA 4 cut(s) 31, 271, 756, 1078
Hpy188III TCNNGA 5 cut(s) 35, 131, 149, 458, 871
Hpy8I GTNNAC 3 cut(s) 94, 888, 1183
Hpy99I CGWCG 2 cut(s) 428, 731
HpyAV CCTTC 4 cut(s) 295, 493, 907, 1105
HpyCH4III ACNGT 1 cut(s) 1060
HpyCH4V TGCA 2 cut(s) 511, 709
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 706
HpyF3I CTNAG 2 cut(s) 296, 614
Hsp92I GRCGYC 1 cut(s) 314
Hsp92II CATG 5 cut(s) 152, 582, 630, 952, 1138
HspAI GCGC 4 cut(s) 217, 314, 364, 897
KasI GGCGCC 1 cut(s) 313
Ksp22I TGATCA 1 cut(s) 867
Kzo9I GATC 4 cut(s) 31, 151, 822, 867
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 5 cut(s) 16, 224, 289, 361, 945
Lsp1109I GCAGC 4 cut(s) 353, 413, 693, 798
LweI GCATC 3 cut(s) 124, 808, 967
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 557, 917, 1054
MalI GATC 4 cut(s) 33, 153, 824, 869
MbiI CCGCTC 2 cut(s) 19, 292
MboI GATC 4 cut(s) 31, 151, 822, 867
MboII GAAGA 5 cut(s) 146, 245, 388, 473, 632
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 6 cut(s) 41, 411, 533, 646, 847, 1081
Mly113I GGCGCC 1 cut(s) 314
MseI TTAA 1 cut(s) 735
MspA1I CMGCKG 2 cut(s) 669, 706
MspR9I CCNGG 1 cut(s) 1100
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 1 cut(s) 1100
MvnI CGCG 2 cut(s) 123, 280
MwoI GCNNNNNNNGC 2 cut(s) 55, 706
NarI GGCGCC 1 cut(s) 314
NcoI CCATGG 1 cut(s) 626
NdeI CATATG 2 cut(s) 439, 791
NdeII GATC 4 cut(s) 31, 151, 822, 867
NlaIII CATG 5 cut(s) 152, 582, 630, 952, 1138
NlaIV GGNNCC 5 cut(s) 194, 315, 572, 632, 984
NmuCI GTSAC 2 cut(s) 557, 1054
NsbI TGCGCA 1 cut(s) 898
NspI RCATGY 1 cut(s) 952
PaeR7I CTCGAG 1 cut(s) 59
PagI TCATGA 1 cut(s) 148
PaqCI CACCTGC 1 cut(s) 345
PciSI GCTCTTC 1 cut(s) 615
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 102
PctI GAATGC 1 cut(s) 709
PfeI GAWTC 3 cut(s) 266, 662, 925
Pfl23II CGTACG 1 cut(s) 940
PflMI CCANNNNNTGG 1 cut(s) 1135
PfoI TCCNGGA 1 cut(s) 1098
PkrI GCNGC 7 cut(s) 294, 368, 403, 484, 530, 708, 788
PluTI GGCGCC 1 cut(s) 317
PsiI TTATAA 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 1098
PspFI CCCAGC 2 cut(s) 546, 702
PspGI CCWGG 1 cut(s) 1098
PspLI CGTACG 1 cut(s) 940
PspN4I GGNNCC 5 cut(s) 194, 315, 572, 632, 984
PspPI GGNCC 1 cut(s) 721
PspXI VCTCGAGB 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 706
RsaI GTAC 2 cut(s) 383, 942
RsaNI GTAC 2 cut(s) 382, 941
SacI GAGCTC 1 cut(s) 66
SalI GTCGAC 1 cut(s) 1181
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 7 cut(s) 293, 367, 402, 483, 529, 707, 787
Sau3AI GATC 4 cut(s) 31, 151, 822, 867
Sau96I GGNCC 1 cut(s) 721
ScaI AGTACT 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 1100
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 3 cut(s) 124, 808, 967
SfcI CTRYAG 1 cut(s) 1176
SfoI GGCGCC 1 cut(s) 315
Sfr274I CTCGAG 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 59
SmlI CTYRAG 2 cut(s) 59, 65
SmoI CTYRAG 2 cut(s) 59, 65
Sse9I AATT 6 cut(s) 41, 411, 533, 646, 847, 1081
SspDI GGCGCC 1 cut(s) 313
SspMI CTAG 1 cut(s) 333
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 1098
StyI CCWWGG 2 cut(s) 197, 626
TaaI ACNGT 1 cut(s) 1060
TaqI TCGA 3 cut(s) 60, 726, 1182
TasI AATT 6 cut(s) 41, 411, 533, 646, 847, 1081
TatI WGTACW 1 cut(s) 381
TauI GCSGC 3 cut(s) 295, 485, 531
TfiI GAWTC 3 cut(s) 266, 662, 925
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 2 cut(s) 557, 1054
TseI GCWGC 4 cut(s) 366, 401, 706, 786
Tsp45I GTSAC 2 cut(s) 557, 1054
TspDTI ATGAA 3 cut(s) 624, 659, 680
TspGWI ACGGA 1 cut(s) 958
TspRI CASTG 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 1135
XagI CCTNNNNNAGG 1 cut(s) 1047
XapI RAATTY 4 cut(s) 533, 646, 847, 1081
XceI RCATGY 1 cut(s) 952
XhoI CTCGAG 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 1182
XspI CTAG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.