Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0014810

Physical Location & Seq

Genomic Coordinates Reverse (-)

6212697 .. 6214668

Gene Structure 1972 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g06140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1902 bp

ATGATGATTCAGATAAATAAACCACCAGTGCTCCTGCCTCCGCCGCCGCCGCAAGGGTGTTTGAAAACGGCTGCTGTTCCGACAAGAGCAGGCCGAAACCTCCTTGTCCTCAACGCACCCACAAGGTCAACAATAATTCCGACAACTATTTCAATGGCTCGTCAAGTCCGACATTCGGTCCTCCCAATAATCCCGATGATGAAAAGAAGAGGAAGAAGAGCAATGGCTGCTTCCGATTCGGTTTCGGCTGCTGCTGTTGTCGACGTGGATATGCAGATTCGGGGGAAGAAAAATAACAAGAATAACAAGGTTTTTAAGGCGTACGGAGACCCCTGCCTCGATCTGTTTTTCAACGTCACACAACGACCCAGCGATTACCGCGAGAAGCAGCGGGAGACGGAGAAGAAGTATCACACCAAAATGTTTAACTATCTGCGGCAACAGCTCCCCTTGGCCTGGTCCCACGATCCTCTCACCACTCTCAAGCTCATCTTCAACCTCTGTCCCTGCGATATCGACGGATCGAAATGGTACAGTGAAGCTTTTGAATTCGCAGCTGTTTGGCTCCACCACAACCACCCCGACACTCTGTTACGCAACTTCGGCTCTCTTGCCGGCTCCTTCGGTCCTTCGTTCACCCTGCTCGACGTTCTGTACGGCCTCCTCGTTCCTCCTCAGGATCAGCTGGATCACACACTGAAGGAGTTACTCCAACGCGAGAATCCGAATTCTCATGCAGATCGGTATGCCCGCGACCCGGCTTACAGGCTGTTGCACGACCGGGGTATGGATGCGTTTGCGGAGCGGTTGAAGTCCGATCTTGAAAAATTGAAGCAGAACAAGCTCGAGCTCAAGCCGTGTGATTATGATGACGATGTTGACGATGATACCGACTACAATGCTAAACGGAACCCATGGGACGCACATAAGGGGCGCTCCATTTCGCTGGAAGAAAGCACTGCGAGGAGGCTTCTCCCCACCGAATCAGATGTATCGCGGGAGTGGGAGCGGCTGAGGAAGGAGGTTTTGGCGCCCTTGAAAAACTACTCTAGTCGTCAAAGTCGGTTTGACAGGTGGAAGCGCTGTGAGGTGAAGAAGTACTTGGAGGAGGTGAAAGCAGCAACAACAATTATAAAGCCCGACGCTTTACTTCCAAATGACATCCTACGCTATCTGGAAGATGGCAATGTTGGGGAAGCAGCAGAGCTTCAATGGAAGGCAATGGTGCAAAAGGTCAAGGAGGGCGGCAAATTCAAAAACTGCTGGGCTGTATGTGACAACGGCTTGTGGCAGTCAAGGAGTTTGAGTTTGGGACTTTTGGTGTATGAACTGAGTGAAGAGCCATGGAACCGAAAAGTGATGAATTTCAGTCTGCCTGATTCCCGCTGTAAGTTTATTCCGCCCATTCATTTTTTGCCCCAGCTGCATTCCATTCAAGGCCTCGACGATGTTAAGTCCAAGTTCTCCTTTCTGACAAGAATGGACAGGAGAATGGACATAAGCATATGTATTGATTTACAAATGGTGTTTGATGCCATCTTGGAAGTGGCTGTCAAGGAGAATTTGAAGCCAGAGCAGATGATCAAGAAGCTGTTTCTGTTCACCGACTGCGCAGGCTATGATTGGAAGGGTTACGAGACTCTGTATGAGGCCATACGGAGCATGTTCGAGGACAAGGGGTATGGGGATGATGCGGTGCCACACATTGTGTTTTGGGATTTTTGGTCGGGGTATCATTCTCCATCCTTCGATTTTCCTCATAGAGGGGTGACGGTGTTGCGTGGCTACTCCAAAAATTTGATCAAATCCTTCTTGGACAATGGTGGGGATTTTCGCCCTCACCATCTCATGGAAGTAGCCATTGCCGACAAAGAATATCAAACTCTTGCTGTAGTCGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF2828

Protein Analysis

634

Amino Acids

73.01

Weight (kDa)

8.43

Isoelectric Point (pI)

39.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF2828	PF11443	103 - 205	5.9e-15	Domain of unknown function (DUF2828)
DUF7788	PF25043	432 - 611	4e-33	Domain of unknown function (DUF7788)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014810)

Species	Orthologous Gene IDs
malus_domestica	MD00G1207400.v1.1 MD03G1055000.v1.1 MD04G1053500.v1.1 MD06G1198700.v1.1 MD07G1028900.v1.1 MD12G1072700.v1.1
prunus_persica	Prupe.1G152600_v2.0.a1 Prupe.2G155300_v2.0.a1 Prupe.5G035500_v2.0.a1 Prupe.5G035900_v2.0.a1 Prupe.5G194100_v2.0.a1 Prupe.8G033200_v2.0.a1 Prupe.8G043600_v2.0.a1
pyrus_communis	pycom04g04610 pycom12g06140 pycom12g06180 pycom15g36970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1133
Acc16I	TGCGCA	1 cut(s)	1612
AccB1I	GGYRCC	2 cut(s)	1030, 1696
AccB7I	CCANNNNNTGG	1 cut(s)	1849
AccBSI	CCGCTC	2 cut(s)	805, 1009
AccI	GTMKAC	2 cut(s)	261, 1896
AccII	CGCG	4 cut(s)	381, 717, 753, 997
AclWI	GGATC	4 cut(s)	461, 529, 687, 696
AcsI	RAATTY	6 cut(s)	548, 727, 1250, 1363, 1561, 1795
AcuI	CTGAAG	1 cut(s)	719
AcyI	GRCGYC	1 cut(s)	1031
AdeI	CACNNNGTG	1 cut(s)	1708
AfaI	GTAC	4 cut(s)	323, 533, 656, 1100
AfeI	AGCGCT	1 cut(s)	1082
AfiI	CCNNNNNNNGG	7 cut(s)	53, 175, 456, 757, 787, 1763, 1849
AhdI	GACNNNNNGTC	1 cut(s)	176
AjiI	CACGTC	1 cut(s)	265
AjnI	CCWGG	1 cut(s)	455
AjuI	GAANNNNNNNTTGG	4 cut(s)	1010, 1042, 1085, 1117
Alw21I	GWGCWC	2 cut(s)	33, 852
Alw26I	GTCTC	3 cut(s)	321, 389, 1631
AlwI	GGATC	4 cut(s)	461, 529, 687, 696
Ama87I	CYCGRG	1 cut(s)	845
Aor51HI	AGCGCT	1 cut(s)	1082
AoxI	GGCC	5 cut(s)	91, 453, 658, 1438, 1650
ApeKI	GCWGC	9 cut(s)	71, 227, 248, 251, 388, 554, 1118, 1199, 1423
ApoI	RAATTY	6 cut(s)	548, 727, 1250, 1363, 1561, 1795
AspLEI	GCGC	4 cut(s)	936, 1033, 1083, 1613
AspS9I	GGNCC	3 cut(s)	178, 459, 626
AsuC2I	CCSGG	2 cut(s)	758, 782
AsuHPI	GGTGA	7 cut(s)	466, 628, 1102, 1123, 1594, 1780, 1832
AvaI	CYCGRG	1 cut(s)	845
AvaII	GGWCC	3 cut(s)	178, 459, 626
AxyI	CCTNAGG	1 cut(s)	675
BanI	GGYRCC	2 cut(s)	1030, 1696
BanII	GRGCYC	1 cut(s)	852
Bbv12I	GWGCWC	2 cut(s)	33, 852
BbvCI	CCTCAGC	1 cut(s)	1013
BbvI	GCAGC	9 cut(s)	58, 214, 235, 238, 400, 566, 1130, 1211, 1410
BccI	CCATC	4 cut(s)	1175, 1544, 1750, 1851
BceAI	ACGGC	4 cut(s)	84, 673, 841, 1297
BcgI	CGANNNNNNTGC	2 cut(s)	881, 915
BciT130I	CCWGG	1 cut(s)	457
BclI	TGATCA	2 cut(s)	1581, 1800
BcnI	CCSGG	2 cut(s)	758, 782
BcoDI	GTCTC	3 cut(s)	321, 389, 1631
BfaI	CTAG	1 cut(s)	1050
BfmI	CTRYAG	1 cut(s)	1890
BfoI	RGCGCY	3 cut(s)	937, 1034, 1084
BmcAI	AGTACT	1 cut(s)	1100
Bme1390I	CCNGG	3 cut(s)	457, 758, 782
Bme18I	GGWCC	3 cut(s)	178, 459, 626
BmeRI	GACNNNNNGTC	1 cut(s)	176
BmeT110I	CYCGRG	1 cut(s)	845
BmgBI	CACGTC	1 cut(s)	265
BmgT120I	GGNCC	3 cut(s)	178, 459, 626
BmiI	GGNNCC	7 cut(s)	461, 566, 619, 911, 1032, 1349, 1698
BmrFI	CCNGG	3 cut(s)	457, 758, 782
BmsI	GCATC	3 cut(s)	781, 1522, 1681
Bpu10I	CCTNAGC	1 cut(s)	1013
BpuEI	CTTGAG	2 cut(s)	467, 836
BpuMI	CCSGG	2 cut(s)	758, 782
BsaHI	GRCGYC	1 cut(s)	1031
BsaI	GGTCTC	1 cut(s)	321
BsaJI	CCNNGG	4 cut(s)	450, 781, 914, 1343
BsaXI	ACNNNNNCTCC	6 cut(s)	15, 45, 392, 422, 1291, 1321
Bsc4I	CCNNNNNNNGG	7 cut(s)	53, 175, 456, 757, 787, 1763, 1849
Bse118I	RCCGGY	1 cut(s)	614
Bse1I	ACTGG	1 cut(s)	26
Bse21I	CCTNAGG	1 cut(s)	675
Bse3DI	GCAATG	4 cut(s)	228, 1192, 1227, 1860
BseBI	CCWGG	1 cut(s)	457
BseDI	CCNNGG	4 cut(s)	450, 781, 914, 1343
BseGI	GGATG	4 cut(s)	796, 1161, 1693, 1742
BseLI	CCNNNNNNNGG	7 cut(s)	53, 175, 456, 757, 787, 1763, 1849
BseMI	GCAATG	4 cut(s)	228, 1192, 1227, 1860
BseMII	CTCAG	3 cut(s)	689, 1004, 1322
BseNI	ACTGG	1 cut(s)	26
BseRI	GAGGAG	4 cut(s)	653, 663, 979, 1121
BseXI	GCAGC	9 cut(s)	58, 214, 235, 238, 400, 566, 1130, 1211, 1410
BseYI	CCCAGC	3 cut(s)	368, 1263, 1419
Bsh1236I	CGCG	4 cut(s)	381, 717, 753, 997
Bsh1285I	CGRYCG	1 cut(s)	781
BshFI	GGCC	5 cut(s)	93, 455, 660, 1440, 1652
BshNI	GGYRCC	2 cut(s)	1030, 1696
BsiEI	CGRYCG	1 cut(s)	781
BsiHKAI	GWGCWC	2 cut(s)	33, 852
BsiHKCI	CYCGRG	1 cut(s)	845
BsiSI	CCGG	3 cut(s)	615, 758, 781
BsiWI	CGTACG	1 cut(s)	321
BslFI	GGGAC	4 cut(s)	445, 489, 932, 1326
BslI	CCNNNNNNNGG	7 cut(s)	53, 175, 456, 757, 787, 1763, 1849
BsmAI	GTCTC	3 cut(s)	321, 389, 1631
BsmBI	CGTCTC	1 cut(s)	389
BsmFI	GGGAC	4 cut(s)	445, 489, 932, 1326
BsmI	GAATGC	1 cut(s)	1426
BsnI	GGCC	5 cut(s)	93, 455, 660, 1440, 1652
Bso31I	GGTCTC	1 cut(s)	321
BsoBI	CYCGRG	1 cut(s)	845
Bsp1286I	GDGCHC	2 cut(s)	33, 852
Bsp143I	GATC	9 cut(s)	340, 466, 521, 679, 688, 739, 817, 1581, 1800
Bsp19I	CCATGG	2 cut(s)	914, 1343
BspANI	GGCC	5 cut(s)	93, 455, 660, 1440, 1652
BspCNI	CTCAG	3 cut(s)	688, 1005, 1323
BspFNI	CGCG	4 cut(s)	381, 717, 753, 997
BspLI	GGNNCC	7 cut(s)	461, 566, 619, 911, 1032, 1349, 1698
BspPI	GGATC	4 cut(s)	461, 529, 687, 696
BspQI	GCTCTTC	2 cut(s)	211, 1332
BspT107I	GGYRCC	2 cut(s)	1030, 1696
BspTNI	GGTCTC	1 cut(s)	321
BsrBI	CCGCTC	2 cut(s)	805, 1009
BsrDI	GCAATG	4 cut(s)	228, 1192, 1227, 1860
BsrFI	RCCGGY	1 cut(s)	614
BsrI	ACTGG	1 cut(s)	26
BssAI	RCCGGY	1 cut(s)	614
BssECI	CCNNGG	4 cut(s)	450, 781, 914, 1343
BssMI	GATC	9 cut(s)	340, 466, 521, 679, 688, 739, 817, 1581, 1800
BssNI	GRCGYC	1 cut(s)	1031
BssT1I	CCWWGG	3 cut(s)	450, 914, 1343
Bst2UI	CCWGG	1 cut(s)	457
Bst4CI	ACNGT	2 cut(s)	536, 1774
Bst6I	CTCTTC	3 cut(s)	202, 211, 1332
BstACI	GRCGYC	1 cut(s)	1031
BstAPI	GCANNNNNTGC	1 cut(s)	227
BstC8I	GCNNGC	4 cut(s)	91, 616, 751, 1615
BstDEI	CTNAG	3 cut(s)	675, 1013, 1331
BstDSI	CCRYGG	2 cut(s)	914, 1343
BstENI	CCTNNNNNAGG	1 cut(s)	1761
BstF5I	GGATG	4 cut(s)	796, 1161, 1693, 1742
BstFNI	CGCG	4 cut(s)	381, 717, 753, 997
BstH2I	RGCGCY	3 cut(s)	937, 1034, 1084
BstHHI	GCGC	4 cut(s)	936, 1033, 1083, 1613
BstKTI	GATC	9 cut(s)	343, 469, 524, 682, 691, 742, 820, 1584, 1803
BstMAI	GTCTC	3 cut(s)	321, 389, 1631
BstMBI	GATC	9 cut(s)	340, 466, 521, 679, 688, 739, 817, 1581, 1800
BstMCI	CGRYCG	1 cut(s)	781
BstMWI	GCNNNNNNNGC	8 cut(s)	43, 49, 227, 378, 442, 603, 841, 1423
BstNI	CCWGG	1 cut(s)	457
BstNSI	RCATGY	1 cut(s)	1666
BstSCI	CCNGG	3 cut(s)	455, 756, 780
BstSFI	CTRYAG	1 cut(s)	1890
BstUI	CGCG	4 cut(s)	381, 717, 753, 997
BstV1I	GCAGC	9 cut(s)	58, 214, 235, 238, 400, 566, 1130, 1211, 1410
BstXI	CCANNNNNNTGG	1 cut(s)	946
Bsu36I	CCTNAGG	1 cut(s)	675
BsuRI	GGCC	5 cut(s)	93, 455, 660, 1440, 1652
BtgI	CCRYGG	2 cut(s)	914, 1343
BtrI	CACGTC	1 cut(s)	265
BtsCI	GGATG	4 cut(s)	796, 1161, 1693, 1742
BtsI	GCAGTG	1 cut(s)	957
BtsIMutI	CAGTG	4 cut(s)	33, 541, 695, 957
Cac8I	GCNNGC	4 cut(s)	91, 616, 751, 1615
CfoI	GCGC	4 cut(s)	936, 1033, 1083, 1613
Cfr10I	RCCGGY	1 cut(s)	614
Cfr13I	GGNCC	3 cut(s)	178, 459, 626
CseI	GACGC	2 cut(s)	929, 1151
Csp6I	GTAC	4 cut(s)	322, 532, 655, 1099
CviAII	CATG	5 cut(s)	734, 915, 1344, 1663, 1849
CviQI	GTAC	4 cut(s)	322, 532, 655, 1099
DdeI	CTNAG	3 cut(s)	675, 1013, 1331
DinI	GGCGCC	1 cut(s)	1032
DpnI	GATC	9 cut(s)	342, 468, 523, 681, 690, 741, 819, 1583, 1802
DpnII	GATC	9 cut(s)	340, 466, 521, 679, 688, 739, 817, 1581, 1800
DraIII	CACNNNGTG	1 cut(s)	1708
DriI	GACNNNNNGTC	1 cut(s)	176
Eam1104I	CTCTTC	3 cut(s)	202, 211, 1332
Eam1105I	GACNNNNNGTC	1 cut(s)	176
EarI	CTCTTC	3 cut(s)	202, 211, 1332
EciI	GGCGGA	2 cut(s)	30, 1389
Ecl136II	GAGCTC	1 cut(s)	850
Eco130I	CCWWGG	3 cut(s)	450, 914, 1343
Eco147I	AGGCCT	1 cut(s)	1440
Eco24I	GRGCYC	1 cut(s)	852
Eco31I	GGTCTC	1 cut(s)	321
Eco32I	GATATC	1 cut(s)	514
Eco47I	GGWCC	3 cut(s)	178, 459, 626
Eco47III	AGCGCT	1 cut(s)	1082
Eco53kI	GAGCTC	1 cut(s)	850
Eco57I	CTGAAG	1 cut(s)	719
Eco81I	CCTNAGG	1 cut(s)	675
Eco88I	CYCGRG	1 cut(s)	845
EcoICRI	GAGCTC	1 cut(s)	850
EcoNI	CCTNNNNNAGG	1 cut(s)	1761
EcoRI	GAATTC	2 cut(s)	548, 727
EcoRII	CCWGG	1 cut(s)	455
EcoRV	GATATC	1 cut(s)	514
EcoT14I	CCWWGG	3 cut(s)	450, 914, 1343
EcoT38I	GRGCYC	1 cut(s)	852
EgeI	GGCGCC	1 cut(s)	1032
EheI	GGCGCC	1 cut(s)	1032
ErhI	CCWWGG	3 cut(s)	450, 914, 1343
Esp3I	CGTCTC	1 cut(s)	389
FaeI	CATG	5 cut(s)	737, 918, 1347, 1666, 1852
FalI	AAGNNNNNCTT	6 cut(s)	476, 508, 1085, 1117, 1451, 1483
FaqI	GGGAC	4 cut(s)	445, 489, 932, 1326
FatI	CATG	5 cut(s)	733, 914, 1343, 1662, 1848
FauI	CCCGC	4 cut(s)	384, 758, 990, 1391
FauNDI	CATATG	1 cut(s)	1505
FbaI	TGATCA	2 cut(s)	1581, 1800
FblI	GTMKAC	2 cut(s)	261, 1896
FokI	GGATG	4 cut(s)	803, 1148, 1700, 1729
FriOI	GRGCYC	1 cut(s)	852
FspBI	CTAG	1 cut(s)	1050
FspI	TGCGCA	1 cut(s)	1612
GlaI	GCGC	4 cut(s)	935, 1032, 1082, 1612
GsaI	CCCAGC	3 cut(s)	372, 1267, 1423
HaeII	RGCGCY	3 cut(s)	937, 1034, 1084
HaeIII	GGCC	5 cut(s)	93, 455, 660, 1440, 1652
HapII	CCGG	3 cut(s)	615, 758, 781
HgaI	GACGC	2 cut(s)	929, 1151
HhaI	GCGC	4 cut(s)	936, 1033, 1083, 1613
Hin1I	GRCGYC	1 cut(s)	1031
Hin1II	CATG	5 cut(s)	737, 918, 1347, 1666, 1852
Hin6I	GCGC	4 cut(s)	934, 1031, 1081, 1611
HinP1I	GCGC	4 cut(s)	934, 1031, 1081, 1611
HincII	GTYRAC	4 cut(s)	129, 262, 880, 1897
HindII	GTYRAC	4 cut(s)	129, 262, 880, 1897
HindIII	AAGCTT	1 cut(s)	540
HinfI	GANTC	7 cut(s)	7, 236, 277, 721, 983, 1379, 1639
HpaII	CCGG	3 cut(s)	615, 758, 781
HphI	GGTGA	7 cut(s)	466, 628, 1102, 1123, 1594, 1780, 1832
Hpy166II	GTNNAC	6 cut(s)	129, 262, 636, 880, 1602, 1897
Hpy188I	TCNGA	9 cut(s)	12, 81, 141, 170, 235, 726, 817, 988, 1473
Hpy188III	TCNNGA	5 cut(s)	193, 677, 821, 1175, 1585
Hpy8I	GTNNAC	6 cut(s)	129, 262, 636, 880, 1602, 1897
Hpy99I	CGWCG	5 cut(s)	266, 521, 650, 1145, 1448
HpyAV	CCTTC	8 cut(s)	631, 639, 694, 1012, 1210, 1621, 1756, 1819
HpyCH4III	ACNGT	2 cut(s)	536, 1774
HpyCH4IV	ACGT	3 cut(s)	264, 354, 648
HpyCH4V	TGCA	5 cut(s)	274, 737, 775, 1228, 1426
HpyF10VI	GCNNNNNNNGC	8 cut(s)	43, 49, 227, 378, 442, 603, 841, 1423
HpyF3I	CTNAG	3 cut(s)	675, 1013, 1331
HpySE526I	ACGT	3 cut(s)	264, 354, 648
Hsp92I	GRCGYC	1 cut(s)	1031
Hsp92II	CATG	5 cut(s)	737, 918, 1347, 1666, 1852
HspAI	GCGC	4 cut(s)	934, 1031, 1081, 1611
KasI	GGCGCC	1 cut(s)	1030
KroI	GCCGGC	1 cut(s)	614
KroNI	GCCGGC	1 cut(s)	616
Ksp22I	TGATCA	2 cut(s)	1581, 1800
Kzo9I	GATC	9 cut(s)	340, 466, 521, 679, 688, 739, 817, 1581, 1800
LguI	GCTCTTC	2 cut(s)	211, 1332
LmnI	GCTCC	8 cut(s)	36, 450, 570, 623, 802, 941, 1006, 1659
Lsp1109I	GCAGC	9 cut(s)	58, 214, 235, 238, 400, 566, 1130, 1211, 1410
LweI	GCATC	3 cut(s)	781, 1522, 1681
MaeI	CTAG	1 cut(s)	1050
MaeII	ACGT	3 cut(s)	264, 354, 648
MaeIII	GTNAC	6 cut(s)	355, 591, 705, 1274, 1631, 1768
MalI	GATC	9 cut(s)	342, 468, 523, 681, 690, 741, 819, 1583, 1802
MbiI	CCGCTC	2 cut(s)	805, 1009
MboI	GATC	9 cut(s)	340, 466, 521, 679, 688, 739, 817, 1581, 1800
MhlI	GDGCHC	2 cut(s)	33, 852
MluCI	AATT	9 cut(s)	135, 548, 727, 827, 1128, 1250, 1363, 1561, 1795
Mly113I	GGCGCC	1 cut(s)	1031
MlyI	GAGTC	1 cut(s)	1633
MmeI	TCCRAC	4 cut(s)	104, 164, 193, 736
MroNI	GCCGGC	1 cut(s)	614
MseI	TTAA	3 cut(s)	315, 426, 1452
MslI	CAYNNNNRTG	1 cut(s)	419
MspA1I	CMGCKG	5 cut(s)	391, 557, 685, 1386, 1423
MspI	CCGG	3 cut(s)	615, 758, 781
MspR9I	CCNGG	3 cut(s)	457, 758, 782
Mva1269I	GAATGC	1 cut(s)	1426
MvaI	CCWGG	1 cut(s)	457
MvnI	CGCG	4 cut(s)	381, 717, 753, 997
MwoI	GCNNNNNNNGC	8 cut(s)	43, 49, 227, 378, 442, 603, 841, 1423
NaeI	GCCGGC	1 cut(s)	616
NarI	GGCGCC	1 cut(s)	1031
NciI	CCSGG	2 cut(s)	758, 782
NcoI	CCATGG	2 cut(s)	914, 1343
NdeI	CATATG	1 cut(s)	1505
NdeII	GATC	9 cut(s)	340, 466, 521, 679, 688, 739, 817, 1581, 1800
NgoMIV	GCCGGC	1 cut(s)	614
NlaIII	CATG	5 cut(s)	737, 918, 1347, 1666, 1852
NlaIV	GGNNCC	7 cut(s)	461, 566, 619, 911, 1032, 1349, 1698
NmuCI	GTSAC	3 cut(s)	355, 1274, 1768
NsbI	TGCGCA	1 cut(s)	1612
NspI	RCATGY	1 cut(s)	1666
PaeR7I	CTCGAG	1 cut(s)	845
PceI	AGGCCT	1 cut(s)	1440
PciSI	GCTCTTC	2 cut(s)	211, 1332
PcsI	WCGNNNNNNNCGW	4 cut(s)	166, 663, 879, 888
PctI	GAATGC	1 cut(s)	1426
PdiI	GCCGGC	1 cut(s)	616
PfeI	GAWTC	6 cut(s)	7, 236, 277, 721, 983, 1379
Pfl23II	CGTACG	1 cut(s)	321
PflMI	CCANNNNNTGG	1 cut(s)	1849
PleI	GAGTC	1 cut(s)	1633
PluTI	GGCGCC	1 cut(s)	1034
PpsI	GAGTC	1 cut(s)	1633
PsiI	TTATAA	1 cut(s)	1133
Psp124BI	GAGCTC	1 cut(s)	852
Psp6I	CCWGG	1 cut(s)	455
PspFI	CCCAGC	3 cut(s)	368, 1263, 1419
PspGI	CCWGG	1 cut(s)	455
PspLI	CGTACG	1 cut(s)	321
PspN4I	GGNNCC	7 cut(s)	461, 566, 619, 911, 1032, 1349, 1698
PspPI	GGNCC	3 cut(s)	178, 459, 626
PspXI	VCTCGAGB	1 cut(s)	845
PvuII	CAGCTG	3 cut(s)	557, 685, 1423
RsaI	GTAC	4 cut(s)	323, 533, 656, 1100
RsaNI	GTAC	4 cut(s)	322, 532, 655, 1099
RseI	CAYNNNNRTG	1 cut(s)	419
SacI	GAGCTC	1 cut(s)	852
SalI	GTCGAC	2 cut(s)	260, 1895
SapI	GCTCTTC	2 cut(s)	211, 1332
SaqAI	TTAA	3 cut(s)	315, 426, 1452
Sau3AI	GATC	9 cut(s)	340, 466, 521, 679, 688, 739, 817, 1581, 1800
Sau96I	GGNCC	3 cut(s)	178, 459, 626
ScaI	AGTACT	1 cut(s)	1100
SchI	GAGTC	1 cut(s)	1633
ScrFI	CCNGG	3 cut(s)	457, 758, 782
SduI	GDGCHC	2 cut(s)	33, 852
SfaNI	GCATC	3 cut(s)	781, 1522, 1681
SfcI	CTRYAG	1 cut(s)	1890
SfoI	GGCGCC	1 cut(s)	1032
Sfr274I	CTCGAG	1 cut(s)	845
SinI	GGWCC	3 cut(s)	178, 459, 626
SlaI	CTCGAG	1 cut(s)	845
SmiMI	CAYNNNNRTG	1 cut(s)	419
SmlI	CTYRAG	3 cut(s)	482, 845, 851
SmoI	CTYRAG	3 cut(s)	482, 845, 851
Sse9I	AATT	9 cut(s)	135, 548, 727, 827, 1128, 1250, 1363, 1561, 1795
SseBI	AGGCCT	1 cut(s)	1440
SspDI	GGCGCC	1 cut(s)	1030
SspMI	CTAG	1 cut(s)	1050
SstI	GAGCTC	1 cut(s)	852
StuI	AGGCCT	1 cut(s)	1440
StyD4I	CCNGG	3 cut(s)	455, 756, 780
StyI	CCWWGG	3 cut(s)	450, 914, 1343
TaaI	ACNGT	2 cut(s)	536, 1774
TaiI	ACGT	3 cut(s)	267, 357, 651
TaqII	GACCGA	2 cut(s)	166, 614
TasI	AATT	9 cut(s)	135, 548, 727, 827, 1128, 1250, 1363, 1561, 1795
TatI	WGTACW	1 cut(s)	1098
TauI	GCSGC	6 cut(s)	46, 49, 52, 439, 1012, 1248
TfiI	GAWTC	6 cut(s)	7, 236, 277, 721, 983, 1379
Tru1I	TTAA	3 cut(s)	315, 426, 1452
Tru9I	TTAA	3 cut(s)	315, 426, 1452
TscAI	CASTG	4 cut(s)	33, 541, 702, 964
TseFI	GTSAC	3 cut(s)	355, 1274, 1768
TseI	GCWGC	9 cut(s)	71, 227, 248, 251, 388, 554, 1118, 1199, 1423
Tsp45I	GTSAC	3 cut(s)	355, 1274, 1768
TspDTI	ATGAA	4 cut(s)	215, 1341, 1376, 1397
TspGWI	ACGGA	5 cut(s)	339, 413, 534, 922, 1672
TspRI	CASTG	4 cut(s)	33, 541, 702, 964
Van91I	CCANNNNNTGG	1 cut(s)	1849
VpaK11BI	GGWCC	3 cut(s)	178, 459, 626
XagI	CCTNNNNNAGG	1 cut(s)	1761
XapI	RAATTY	6 cut(s)	548, 727, 1250, 1363, 1561, 1795
XceI	RCATGY	1 cut(s)	1666
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1543
XhoI	CTCGAG	1 cut(s)	845
XmiI	GTMKAC	2 cut(s)	261, 1896
XspI	CTAG	1 cut(s)	1050
ZrmI	AGTACT	1 cut(s)	1100

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.