MD07G1028900.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014810
Physical Location & Seq
Reverse (-)
2400301 .. 2401885
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1028900.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGGGGATTCGGAGGAAGAAGAATAACAAGAATAACAAGGTTTTTAAGGCGTACGGCGACCCCTGCCTCGATCTGTTTTTCAACGTCACACAACGACCCGGCGATTACAGCGAGGAGCAGCAGGAGACGGAGAAGGAGTATCACACCAAAATGTTTAACTATCTGCGGCAACAGCTCCCCTTGGCCTGGTCCCACGATCCTCTCACCACTCTCAAGCTCATCTTCAACCTCTCTTCCTGCGGTATCGACGGATCGAAATGGTACAGTGAAGCTTTTGAATTCGCAGCTGTTTGGCTCCACCACAACCACCCCAACACTCTGTTACGCAACTTCGGCTCTCTTGCCGACTCCTTCGGTCCTTTGTTCACCCTGCTCGACGTTCTGTACGGCATCCTCGTTCCTCCTCAGGATCAGCTGGATCACACACTGGAGGAGTTACTCCAACGCGAGAATCCGAATTCTCATGCTGATCGGTACGCCCACGACCCGACTTACAGGCTTTTGCACGACCGGGGTATGGATGCTTTTGCGGAGCGGTTGAAGTCCGATCTTGAAAAATTGAAGCAGAACAAGCTCGAGCTCAAGCCGTGTGATTATGATGACGATGTTGACGATGTTGACGATGATACCGACTACAATGCTAAACGCGGTTCTCTTGATGCTTATGCTTATCATGATCTTCTCGTTTCTTTGGCAATAGATTGTTTGCTTACAGGGAACCCTTGGGAGGCACATAAGGGGCGCTCCATTTCGCTGGAAGAAAGCACTGCGAGGAGGCTTCTCACCCCCGAATCAGATGTATCGCGGGAGTGGGAGCGGCTGAGGAAGGAGGTTTTGGCGCCCTTGAAAAACTACTCTAGTCGTCAAAGTCGGTTTGGCAGGTGGGAGCGCTGCGAGGTGAAGAAGTACTTGGAGGAGGTGAAAGCAGCAACAACAATTATAAAGCCCGACGCTTTACTTCCATATGACATCCTACGCTATCTGGAAGATGGTAATGTTGGGGAAGCGGCAGAGCTTCAATGGAAGGCAATGGTGCAAAAGGTCAAGGAGGGCGGCAAATTCAAAAACTGCTGGGCTAGCCATGGAACCGAAAAAGTGATGAATTTCAGTCTGCCTGATTCCCGCTGTAAGTTTATTCCGCCCATTCATTTTTTGCCCCAGCTGCATTCCATTCAGGGCCTCGACGATGTTAAGTCCAAGTTCTCCTTTCTGACGAGAATGGAGAGGAGAATGGACATAAGCAGCATATGTATTGATATACAAATGGTGTTTGATGCGATCTTGGAAGTTGTTGTCAAGGAGAATTTGAAGCCAGAGCAGATGATCAAGAAGCTGTTTCTGTTCACCGACTGCGCAGGCTATGATTGGAAGGGTTACGAGAATCTGTATGAGGCCGTACGGAGCATGTTCGGGGACAAGGGGTATGGGGATGATGCGGTGCCACACATTGTGTTTTTGGGATTTTTTGGTGGGGGTATCGTTCTCCATCCTTTGATTTTCCTCATAGAGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

505

Amino Acids

58.2

Weight (kDa)

5.65

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 13 - 116 3.1e-16 Domain of unknown function (DUF2828)
DUF7788 PF25043 386 - 485 5.7e-15 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 941
AarI CACCTGC 1 cut(s) 870
Acc16I TGCGCA 1 cut(s) 1354
Acc36I ACCTGC 1 cut(s) 870
AccB1I GGYRCC 2 cut(s) 838, 1438
AccBSI CCGCTC 2 cut(s) 535, 817
AccII CGCG 3 cut(s) 447, 648, 805
AclWI GGATC 4 cut(s) 191, 259, 417, 426
AcsI RAATTY 5 cut(s) 278, 457, 1058, 1102, 1303
AcyI GRCGYC 1 cut(s) 839
AdeI CACNNNGTG 1 cut(s) 1450
AfaI GTAC 6 cut(s) 53, 263, 386, 476, 908, 1398
AfeI AGCGCT 1 cut(s) 890
AfiI CCNNNNNNNGG 4 cut(s) 186, 517, 727, 1507
AjnI CCWGG 1 cut(s) 185
AjuI GAANNNNNNNTTGG 4 cut(s) 818, 850, 893, 925
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 4 cut(s) 191, 259, 417, 426
Ama87I CYCGRG 1 cut(s) 575
Aor51HI AGCGCT 1 cut(s) 890
AoxI GGCC 3 cut(s) 183, 1177, 1392
ApeKI GCWGC 6 cut(s) 118, 284, 891, 926, 1162, 1242
ApoI RAATTY 5 cut(s) 278, 457, 1058, 1102, 1303
AspLEI GCGC 4 cut(s) 744, 841, 891, 1355
AspS9I GGNCC 3 cut(s) 189, 356, 1177
AsuC2I CCSGG 2 cut(s) 99, 512
AsuHPI GGTGA 6 cut(s) 196, 358, 775, 910, 931, 1336
AsuNHI GCTAGC 1 cut(s) 1076
AvaI CYCGRG 1 cut(s) 575
AvaII GGWCC 2 cut(s) 189, 356
AxyI CCTNAGG 1 cut(s) 405
BanI GGYRCC 2 cut(s) 838, 1438
BanII GRGCYC 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 582
BbvCI CCTCAGC 1 cut(s) 821
BbvI GCAGC 6 cut(s) 130, 296, 878, 938, 1149, 1254
BccI CCATC 2 cut(s) 983, 1494
BceAI ACGGC 4 cut(s) 70, 403, 571, 1379
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciT130I CCWGG 1 cut(s) 187
BclI TGATCA 1 cut(s) 1323
BcnI CCSGG 2 cut(s) 99, 512
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 2 cut(s) 858, 1077
BfoI RGCGCY 3 cut(s) 745, 842, 892
BfuAI ACCTGC 1 cut(s) 870
BmcAI AGTACT 1 cut(s) 908
Bme1390I CCNGG 3 cut(s) 99, 187, 512
Bme18I GGWCC 2 cut(s) 189, 356
BmeT110I CYCGRG 1 cut(s) 575
BmgT120I GGNCC 3 cut(s) 189, 356, 1177
BmiI GGNNCC 6 cut(s) 191, 296, 719, 840, 1087, 1440
BmrFI CCNGG 3 cut(s) 99, 187, 512
BmsI GCATC 5 cut(s) 399, 511, 649, 1264, 1423
BmtI GCTAGC 1 cut(s) 1080
BpmI CTGGAG 1 cut(s) 449
Bpu10I CCTNAGC 1 cut(s) 821
BpuEI CTTGAG 2 cut(s) 197, 566
BpuMI CCSGG 2 cut(s) 99, 512
BsaHI GRCGYC 1 cut(s) 839
BsaJI CCNNGG 4 cut(s) 180, 511, 722, 1081
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 4 cut(s) 186, 517, 727, 1507
Bse1I ACTGG 1 cut(s) 432
Bse21I CCTNAGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 1035
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 4 cut(s) 180, 511, 722, 1081
BseGI GGATG 5 cut(s) 390, 526, 969, 1435, 1486
BseLI CCNNNNNNNGG 4 cut(s) 186, 517, 727, 1507
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 2 cut(s) 419, 812
BseNI ACTGG 1 cut(s) 432
BseRI GAGGAG 6 cut(s) 128, 393, 446, 787, 929, 1240
BseXI GCAGC 6 cut(s) 130, 296, 878, 938, 1149, 1254
BseYI CCCAGC 2 cut(s) 1071, 1158
Bsh1236I CGCG 3 cut(s) 447, 648, 805
Bsh1285I CGRYCG 1 cut(s) 511
BshFI GGCC 3 cut(s) 185, 1179, 1394
BshNI GGYRCC 2 cut(s) 838, 1438
BsiEI CGRYCG 1 cut(s) 511
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 575
BsiSI CCGG 2 cut(s) 99, 511
BsiWI CGTACG 2 cut(s) 51, 1396
BslFI GGGAC 2 cut(s) 175, 1427
BslI CCNNNNNNNGG 4 cut(s) 186, 517, 727, 1507
BsmAI GTCTC 1 cut(s) 119
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 2 cut(s) 175, 1427
BsmI GAATGC 1 cut(s) 1165
BsnI GGCC 3 cut(s) 185, 1179, 1394
BsoBI CYCGRG 1 cut(s) 575
Bsp1286I GDGCHC 1 cut(s) 582
Bsp19I CCATGG 1 cut(s) 1081
BspANI GGCC 3 cut(s) 185, 1179, 1394
BspCNI CTCAG 2 cut(s) 418, 813
BspFNI CGCG 3 cut(s) 447, 648, 805
BspHI TCATGA 1 cut(s) 673
BspLI GGNNCC 6 cut(s) 191, 296, 719, 840, 1087, 1440
BspMI ACCTGC 1 cut(s) 870
BspOI GCTAGC 1 cut(s) 1080
BspPI GGATC 4 cut(s) 191, 259, 417, 426
BspT107I GGYRCC 2 cut(s) 838, 1438
BsrBI CCGCTC 2 cut(s) 535, 817
BsrDI GCAATG 1 cut(s) 1035
BsrI ACTGG 1 cut(s) 432
BssECI CCNNGG 4 cut(s) 180, 511, 722, 1081
BssNI GRCGYC 1 cut(s) 839
BssT1I CCWWGG 3 cut(s) 180, 722, 1081
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 1 cut(s) 238
BstACI GRCGYC 1 cut(s) 839
BstC8I GCNNGC 2 cut(s) 1078, 1357
BstDEI CTNAG 2 cut(s) 405, 821
BstDSI CCRYGG 1 cut(s) 1081
BstENI CCTNNNNNAGG 1 cut(s) 1505
BstF5I GGATG 5 cut(s) 390, 526, 969, 1435, 1486
BstFNI CGCG 3 cut(s) 447, 648, 805
BstH2I RGCGCY 3 cut(s) 745, 842, 892
BstHHI GCGC 4 cut(s) 744, 841, 891, 1355
BstMAI GTCTC 1 cut(s) 119
BstMCI CGRYCG 1 cut(s) 511
BstMWI GCNNNNNNNGC 7 cut(s) 63, 108, 172, 333, 571, 1077, 1162
BstNI CCWGG 1 cut(s) 187
BstNSI RCATGY 1 cut(s) 1408
BstSCI CCNGG 3 cut(s) 97, 185, 510
BstUI CGCG 3 cut(s) 447, 648, 805
BstV1I GCAGC 6 cut(s) 130, 296, 878, 938, 1149, 1254
BstXI CCANNNNNNTGG 1 cut(s) 754
Bsu36I CCTNAGG 1 cut(s) 405
BsuRI GGCC 3 cut(s) 185, 1179, 1394
BtgI CCRYGG 1 cut(s) 1081
BtsCI GGATG 5 cut(s) 390, 526, 969, 1435, 1486
BtsI GCAGTG 1 cut(s) 765
BtsIMutI CAGTG 3 cut(s) 271, 425, 765
BveI ACCTGC 1 cut(s) 870
Cac8I GCNNGC 2 cut(s) 1078, 1357
CciI TCATGA 1 cut(s) 673
CfoI GCGC 4 cut(s) 744, 841, 891, 1355
Cfr13I GGNCC 3 cut(s) 189, 356, 1177
CseI GACGC 1 cut(s) 959
Csp6I GTAC 6 cut(s) 52, 262, 385, 475, 907, 1397
CviAII CATG 4 cut(s) 464, 674, 1082, 1405
CviQI GTAC 6 cut(s) 52, 262, 385, 475, 907, 1397
DdeI CTNAG 2 cut(s) 405, 821
DinI GGCGCC 1 cut(s) 840
DraIII CACNNNGTG 1 cut(s) 1450
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
EciI GGCGGA 1 cut(s) 1128
Ecl136II GAGCTC 1 cut(s) 580
Eco130I CCWWGG 3 cut(s) 180, 722, 1081
Eco24I GRGCYC 1 cut(s) 582
Eco47I GGWCC 2 cut(s) 189, 356
Eco47III AGCGCT 1 cut(s) 890
Eco53kI GAGCTC 1 cut(s) 580
Eco81I CCTNAGG 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 575
EcoICRI GAGCTC 1 cut(s) 580
EcoNI CCTNNNNNAGG 1 cut(s) 1505
EcoO109I RGGNCCY 1 cut(s) 1177
EcoRI GAATTC 2 cut(s) 278, 457
EcoRII CCWGG 1 cut(s) 185
EcoT14I CCWWGG 3 cut(s) 180, 722, 1081
EcoT38I GRGCYC 1 cut(s) 582
EgeI GGCGCC 1 cut(s) 840
EheI GGCGCC 1 cut(s) 840
ErhI CCWWGG 3 cut(s) 180, 722, 1081
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 4 cut(s) 467, 677, 1085, 1408
FalI AAGNNNNNCTT 6 cut(s) 206, 238, 893, 925, 1190, 1222
FaqI GGGAC 2 cut(s) 175, 1427
FatI CATG 4 cut(s) 463, 673, 1081, 1404
FauI CCCGC 2 cut(s) 798, 1130
FauNDI CATATG 2 cut(s) 964, 1247
FbaI TGATCA 1 cut(s) 1323
FokI GGATG 5 cut(s) 377, 533, 956, 1442, 1473
FriOI GRGCYC 1 cut(s) 582
FspBI CTAG 2 cut(s) 858, 1077
FspI TGCGCA 1 cut(s) 1354
GlaI GCGC 4 cut(s) 743, 840, 890, 1354
GsaI CCCAGC 2 cut(s) 1075, 1162
GsuI CTGGAG 1 cut(s) 449
HaeII RGCGCY 3 cut(s) 745, 842, 892
HaeIII GGCC 3 cut(s) 185, 1179, 1394
HapII CCGG 2 cut(s) 99, 511
HgaI GACGC 1 cut(s) 959
HhaI GCGC 4 cut(s) 744, 841, 891, 1355
Hin1I GRCGYC 1 cut(s) 839
Hin1II CATG 4 cut(s) 467, 677, 1085, 1408
Hin6I GCGC 4 cut(s) 742, 839, 889, 1353
HinP1I GCGC 4 cut(s) 742, 839, 889, 1353
HincII GTYRAC 2 cut(s) 610, 619
HindII GTYRAC 2 cut(s) 610, 619
HindIII AAGCTT 1 cut(s) 270
HinfI GANTC 6 cut(s) 7, 347, 451, 791, 1118, 1381
HpaII CCGG 2 cut(s) 99, 511
HphI GGTGA 6 cut(s) 196, 358, 775, 910, 931, 1336
Hpy166II GTNNAC 4 cut(s) 366, 610, 619, 1344
Hpy188I TCNGA 5 cut(s) 12, 456, 547, 796, 1212
Hpy188III TCNNGA 6 cut(s) 407, 551, 656, 674, 983, 1327
Hpy8I GTNNAC 4 cut(s) 366, 610, 619, 1344
Hpy99I CGWCG 4 cut(s) 251, 380, 953, 1187
HpyAV CCTTC 5 cut(s) 127, 361, 820, 1018, 1363
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4IV ACGT 2 cut(s) 84, 378
HpyCH4V TGCA 3 cut(s) 505, 1036, 1165
HpyF10VI GCNNNNNNNGC 7 cut(s) 63, 108, 172, 333, 571, 1077, 1162
HpyF3I CTNAG 2 cut(s) 405, 821
HpySE526I ACGT 2 cut(s) 84, 378
Hsp92I GRCGYC 1 cut(s) 839
Hsp92II CATG 4 cut(s) 467, 677, 1085, 1408
HspAI GCGC 4 cut(s) 742, 839, 889, 1353
KasI GGCGCC 1 cut(s) 838
Ksp22I TGATCA 1 cut(s) 1323
LmnI GCTCC 8 cut(s) 115, 180, 300, 532, 749, 814, 886, 1401
Lsp1109I GCAGC 6 cut(s) 130, 296, 878, 938, 1149, 1254
LweI GCATC 5 cut(s) 399, 511, 649, 1264, 1423
MaeI CTAG 2 cut(s) 858, 1077
MaeII ACGT 2 cut(s) 84, 378
MaeIII GTNAC 4 cut(s) 85, 321, 435, 1373
MbiI CCGCTC 2 cut(s) 535, 817
MboII GAAGA 8 cut(s) 28, 31, 214, 225, 671, 770, 913, 998
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 7 cut(s) 278, 457, 557, 936, 1058, 1102, 1303
Mly113I GGCGCC 1 cut(s) 839
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 466
MseI TTAA 3 cut(s) 45, 156, 1191
MslI CAYNNNNRTG 1 cut(s) 149
MspA1I CMGCKG 4 cut(s) 287, 415, 1125, 1162
MspI CCGG 2 cut(s) 99, 511
MspR9I CCNGG 3 cut(s) 99, 187, 512
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 1 cut(s) 187
MvnI CGCG 3 cut(s) 447, 648, 805
MwoI GCNNNNNNNGC 7 cut(s) 63, 108, 172, 333, 571, 1077, 1162
NarI GGCGCC 1 cut(s) 839
NciI CCSGG 2 cut(s) 99, 512
NcoI CCATGG 1 cut(s) 1081
NdeI CATATG 2 cut(s) 964, 1247
NheI GCTAGC 1 cut(s) 1076
NlaIII CATG 4 cut(s) 467, 677, 1085, 1408
NlaIV GGNNCC 6 cut(s) 191, 296, 719, 840, 1087, 1440
NmuCI GTSAC 1 cut(s) 85
NsbI TGCGCA 1 cut(s) 1354
NspI RCATGY 1 cut(s) 1408
PaeR7I CTCGAG 1 cut(s) 575
PagI TCATGA 1 cut(s) 673
PaqCI CACCTGC 1 cut(s) 870
PcsI WCGNNNNNNNCGW 4 cut(s) 393, 609, 618, 627
PctI GAATGC 1 cut(s) 1165
PfeI GAWTC 5 cut(s) 7, 451, 791, 1118, 1381
Pfl23II CGTACG 2 cut(s) 51, 1396
PleI GAGTC 1 cut(s) 341
PluTI GGCGCC 1 cut(s) 842
PpsI GAGTC 1 cut(s) 341
PsiI TTATAA 1 cut(s) 941
Psp124BI GAGCTC 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 185
PspFI CCCAGC 2 cut(s) 1071, 1158
PspGI CCWGG 1 cut(s) 185
PspLI CGTACG 2 cut(s) 51, 1396
PspN4I GGNNCC 6 cut(s) 191, 296, 719, 840, 1087, 1440
PspPI GGNCC 3 cut(s) 189, 356, 1177
PspXI VCTCGAGB 1 cut(s) 575
PvuII CAGCTG 3 cut(s) 287, 415, 1162
RsaI GTAC 6 cut(s) 53, 263, 386, 476, 908, 1398
RsaNI GTAC 6 cut(s) 52, 262, 385, 475, 907, 1397
RseI CAYNNNNRTG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 582
SaqAI TTAA 3 cut(s) 45, 156, 1191
Sau96I GGNCC 3 cut(s) 189, 356, 1177
ScaI AGTACT 1 cut(s) 908
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 3 cut(s) 99, 187, 512
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 5 cut(s) 399, 511, 649, 1264, 1423
SfoI GGCGCC 1 cut(s) 840
Sfr274I CTCGAG 1 cut(s) 575
SinI GGWCC 2 cut(s) 189, 356
SlaI CTCGAG 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 3 cut(s) 212, 575, 581
SmoI CTYRAG 3 cut(s) 212, 575, 581
Sse9I AATT 7 cut(s) 278, 457, 557, 936, 1058, 1102, 1303
SspDI GGCGCC 1 cut(s) 838
SspMI CTAG 2 cut(s) 858, 1077
SstI GAGCTC 1 cut(s) 582
StyD4I CCNGG 3 cut(s) 97, 185, 510
StyI CCWWGG 3 cut(s) 180, 722, 1081
TaaI ACNGT 1 cut(s) 266
TaiI ACGT 2 cut(s) 87, 381
TaqI TCGA 6 cut(s) 69, 246, 254, 375, 576, 1182
TaqII GACCGA 1 cut(s) 344
TasI AATT 7 cut(s) 278, 457, 557, 936, 1058, 1102, 1303
TatI WGTACW 1 cut(s) 906
TauI GCSGC 4 cut(s) 169, 820, 1010, 1056
TfiI GAWTC 5 cut(s) 7, 451, 791, 1118, 1381
Tru1I TTAA 3 cut(s) 45, 156, 1191
Tru9I TTAA 3 cut(s) 45, 156, 1191
TscAI CASTG 3 cut(s) 271, 432, 772
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 6 cut(s) 118, 284, 891, 926, 1162, 1242
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 1115, 1136
TspGWI ACGGA 3 cut(s) 143, 264, 1414
TspRI CASTG 3 cut(s) 271, 432, 772
VpaK11BI GGWCC 2 cut(s) 189, 356
XagI CCTNNNNNAGG 1 cut(s) 1505
XapI RAATTY 5 cut(s) 278, 457, 1058, 1102, 1303
XceI RCATGY 1 cut(s) 1408
XhoI CTCGAG 1 cut(s) 575
XspI CTAG 2 cut(s) 858, 1077
ZrmI AGTACT 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.