pycom04g04610

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014810
Physical Location & Seq
Forward (+)
4221140 .. 4221909
770 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g04610.3

Sequence Viewer

Length: 645 bp
ATGCTTTGCTTCCACACTTTATCATACGATATTTGCACGATGCAAATGTTGGGGAGGCAGCTGAGCTTCAGTGGAATGCATTGCTGCATAAGCACAAGGAGGGTGGCATTCAAAAACTGGTTTGCTTTATGTAACATGGACATAATTATGGGAAAACTGATCAGTTCCGCTTCTTTTCCGGTTGGTGCCGGAGACAACCACGCTCTGTTGCTTCATCCTCCTCATCTGTATTCAGTACCCTGCGACGATGGCCTAAGGTCCAAATTGTCTTTTTTAATGGGAATGGACGGCTGCATGAATTTGAAGCCATATCAGATGATCCAGAAACTCTTTATCTTCAACAACAACATGGGCCCCGATTTTTATGGTGGCGCGGCCTGGGAGACTCACTACGAGGCCATAAGGACCAAGTTTAAGGACAAAGGGTACAGGGATGATGCGGTGCCTCACATTCTGTTTTGGGATATTTGGTACTGGGAATTGCCCTCAATTGCTTTGCCTCGTCCAGGGGTGACGCTGCTGCGTGGCTGGTCTAATAATTTGGTGAAGTCCTTCTTGGAGAATGGTGGGGATATTGGCTCGCACGATGTCATGGAAGCAACCTTCTCGGACAAAAAGTTTCAAGCTTTCGCTGTTGTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

215

Amino Acids

24.31

Weight (kDa)

6.69

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 185, 442
AccII CGCG 1 cut(s) 374
AciI CCGC 3 cut(s) 168, 374, 440
AclWI GGATC 1 cut(s) 313
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 52
AfaI GTAC 3 cut(s) 237, 428, 473
AfiI CCNNNNNNNGG 1 cut(s) 506
AgsI TTSAA 4 cut(s) 112, 304, 340, 623
AjnI CCWGG 2 cut(s) 377, 505
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AluBI AGCT 3 cut(s) 61, 66, 626
AluI AGCT 3 cut(s) 61, 66, 626
Alw26I GTCTC 2 cut(s) 186, 377
AlwI GGATC 1 cut(s) 313
AoxI GGCC 4 cut(s) 250, 352, 375, 396
ApaI GGGCCC 1 cut(s) 356
ApeKI GCWGC 5 cut(s) 58, 84, 291, 517, 520
ApoI RAATTY 1 cut(s) 298
Asp700I GAANNNNTTC 1 cut(s) 551
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 4 cut(s) 258, 352, 353, 405
AsuHPI GGTGA 2 cut(s) 523, 556
AvaII GGWCC 2 cut(s) 258, 405
AxyI CCTNAGG 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 356
BanI GGYRCC 2 cut(s) 185, 442
BanII GRGCYC 1 cut(s) 356
BbvI GCAGC 5 cut(s) 70, 71, 278, 504, 507
BccI CCATC 1 cut(s) 242
BceAI ACGGC 1 cut(s) 304
BcgI CGANNNNNNTGC 2 cut(s) 588, 622
BciT130I CCWGG 2 cut(s) 379, 507
BclI TGATCA 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 186, 377
BisI GCNGC 6 cut(s) 59, 85, 292, 375, 518, 521
BlpI GCTNAGC 1 cut(s) 62
BlsI GCNGC 6 cut(s) 60, 86, 293, 376, 519, 522
Bme1390I CCNGG 2 cut(s) 379, 507
Bme18I GGWCC 2 cut(s) 258, 405
BmgT120I GGNCC 4 cut(s) 258, 352, 353, 405
BmiI GGNNCC 4 cut(s) 187, 354, 355, 444
BmrFI CCNGG 2 cut(s) 379, 507
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 2 cut(s) 30, 427
BmuI ACTGGG 1 cut(s) 484
Bpu1102I GCTNAGC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 378, 506
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse1I ACTGG 2 cut(s) 122, 479
Bse21I CCTNAGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 79
BseBI CCWGG 2 cut(s) 379, 507
BseDI CCNNGG 2 cut(s) 378, 506
BseGI GGATG 2 cut(s) 214, 439
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 2 cut(s) 122, 479
BseRI GAGGAG 1 cut(s) 210
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 5 cut(s) 70, 71, 278, 504, 507
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 4 cut(s) 252, 354, 377, 398
BshNI GGYRCC 2 cut(s) 185, 442
BsiSI CCGG 2 cut(s) 179, 189
BslI CCNNNNNNNGG 1 cut(s) 506
BsmAI GTCTC 2 cut(s) 186, 377
BsmI GAATGC 2 cut(s) 81, 107
BsnI GGCC 4 cut(s) 252, 354, 377, 398
Bsp120I GGGCCC 1 cut(s) 352
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 2 cut(s) 159, 318
Bsp1720I GCTNAGC 1 cut(s) 62
BspACI CCGC 3 cut(s) 168, 374, 440
BspANI GGCC 4 cut(s) 252, 354, 377, 398
BspCNI CTCAG 1 cut(s) 54
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 4 cut(s) 187, 354, 355, 444
BspPI GGATC 1 cut(s) 313
BspT107I GGYRCC 2 cut(s) 185, 442
BsrDI GCAATG 1 cut(s) 79
BsrI ACTGG 2 cut(s) 122, 479
BssECI CCNNGG 2 cut(s) 378, 506
BssMI GATC 2 cut(s) 159, 318
Bst2UI CCWGG 2 cut(s) 379, 507
BstC8I GCNNGC 1 cut(s) 581
BstDEI CTNAG 2 cut(s) 62, 254
BstENI CCTNNNNNAGG 1 cut(s) 504
BstF5I GGATG 2 cut(s) 214, 439
BstFNI CGCG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 2 cut(s) 162, 321
BstMAI GTCTC 2 cut(s) 186, 377
BstMBI GATC 2 cut(s) 159, 318
BstMWI GCNNNNNNNGC 2 cut(s) 90, 249
BstNI CCWGG 2 cut(s) 379, 507
BstSCI CCNGG 2 cut(s) 377, 505
BstSLI GKGCMC 1 cut(s) 356
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 5 cut(s) 70, 71, 278, 504, 507
Bsu36I CCTNAGG 1 cut(s) 254
BsuRI GGCC 4 cut(s) 252, 354, 377, 398
BtsCI GGATG 2 cut(s) 214, 439
BtsIMutI CAGTG 1 cut(s) 76
Cac8I GCNNGC 1 cut(s) 581
CfoI GCGC 1 cut(s) 374
Cfr13I GGNCC 4 cut(s) 258, 352, 353, 405
CseI GACGC 1 cut(s) 523
Csp6I GTAC 3 cut(s) 236, 427, 472
CspCI CAANNNNNGTGG 2 cut(s) 84, 119
CviAII CATG 4 cut(s) 136, 295, 349, 592
CviQI GTAC 3 cut(s) 236, 427, 472
DdeI CTNAG 2 cut(s) 62, 254
DpnI GATC 2 cut(s) 161, 320
DpnII GATC 2 cut(s) 159, 318
Eco24I GRGCYC 1 cut(s) 356
Eco47I GGWCC 2 cut(s) 258, 405
Eco57I CTGAAG 1 cut(s) 52
Eco81I CCTNAGG 1 cut(s) 254
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoO109I RGGNCCY 1 cut(s) 353
EcoRII CCWGG 2 cut(s) 377, 505
EcoT22I ATGCAT 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 356
FaeI CATG 4 cut(s) 139, 298, 352, 595
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FatI CATG 4 cut(s) 135, 294, 348, 591
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 6 cut(s) 59, 85, 292, 375, 518, 521
FokI GGATG 2 cut(s) 201, 446
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 6 cut(s) 59, 85, 292, 375, 518, 521
GlaI GCGC 1 cut(s) 373
GluI GCNGC 6 cut(s) 59, 85, 292, 375, 518, 521
HaeIII GGCC 4 cut(s) 252, 354, 377, 398
HapII CCGG 2 cut(s) 179, 189
HgaI GACGC 1 cut(s) 523
HhaI GCGC 1 cut(s) 374
Hin1II CATG 4 cut(s) 139, 298, 352, 595
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 1 cut(s) 385
HpaII CCGG 2 cut(s) 179, 189
HphI GGTGA 2 cut(s) 523, 556
Hpy166II GTNNAC 1 cut(s) 640
Hpy188I TCNGA 2 cut(s) 315, 610
Hpy188III TCNNGA 1 cut(s) 322
Hpy8I GTNNAC 1 cut(s) 640
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 2 cut(s) 562, 613
HpyCH4V TGCA 5 cut(s) 36, 43, 79, 87, 294
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 249
HpyF3I CTNAG 2 cut(s) 62, 254
Hsp92II CATG 4 cut(s) 139, 298, 352, 595
HspAI GCGC 1 cut(s) 372
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 2 cut(s) 159, 318
Lsp1109I GCAGC 5 cut(s) 70, 71, 278, 504, 507
LweI GCATC 2 cut(s) 30, 427
MaeIII GTNAC 2 cut(s) 131, 511
MalI GATC 2 cut(s) 161, 320
MboI GATC 2 cut(s) 159, 318
MboII GAAGA 1 cut(s) 328
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 6 cut(s) 144, 263, 298, 479, 489, 538
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 8 cut(s) 48, 93, 228, 231, 388, 456, 496, 510
Mph1103I ATGCAT 1 cut(s) 81
MroXI GAANNNNTTC 1 cut(s) 551
MseI TTAA 2 cut(s) 275, 414
MslI CAYNNNNRTG 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 2 cut(s) 179, 189
MspR9I CCNGG 2 cut(s) 379, 507
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 2 cut(s) 81, 107
MvaI CCWGG 2 cut(s) 379, 507
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 2 cut(s) 90, 249
NdeII GATC 2 cut(s) 159, 318
NlaIII CATG 4 cut(s) 139, 298, 352, 595
NlaIV GGNNCC 4 cut(s) 187, 354, 355, 444
NmuCI GTSAC 1 cut(s) 511
NsiI ATGCAT 1 cut(s) 81
PctI GAATGC 2 cut(s) 81, 107
PdmI GAANNNNTTC 1 cut(s) 551
PkrI GCNGC 6 cut(s) 60, 86, 293, 376, 519, 522
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 2 cut(s) 377, 505
PspGI CCWGG 2 cut(s) 377, 505
PspN4I GGNNCC 4 cut(s) 187, 354, 355, 444
PspOMI GGGCCC 1 cut(s) 352
PspPI GGNCC 4 cut(s) 258, 352, 353, 405
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 3 cut(s) 237, 428, 473
RsaNI GTAC 3 cut(s) 236, 427, 472
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 2 cut(s) 275, 414
SatI GCNGC 6 cut(s) 59, 85, 292, 375, 518, 521
Sau3AI GATC 2 cut(s) 159, 318
Sau96I GGNCC 4 cut(s) 258, 352, 353, 405
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 379, 507
SduI GDGCHC 1 cut(s) 356
SetI ASST 5 cut(s) 63, 68, 260, 605, 628
SfaNI GCATC 2 cut(s) 30, 427
SinI GGWCC 2 cut(s) 258, 405
SmiMI CAYNNNNRTG 1 cut(s) 146
Sse9I AATT 6 cut(s) 144, 263, 298, 479, 489, 538
SsiI CCGC 3 cut(s) 168, 374, 440
StyD4I CCNGG 2 cut(s) 377, 505
TasI AATT 6 cut(s) 144, 263, 298, 479, 489, 538
TauI GCSGC 1 cut(s) 377
Tru1I TTAA 2 cut(s) 275, 414
Tru9I TTAA 2 cut(s) 275, 414
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 5 cut(s) 58, 84, 291, 517, 520
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 2 cut(s) 203, 311
TspRI CASTG 1 cut(s) 76
VpaK11BI GGWCC 2 cut(s) 258, 405
XagI CCTNNNNNAGG 1 cut(s) 504
XapI RAATTY 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 551
Zsp2I ATGCAT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.