Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014344

Physical Location & Seq

Genomic Coordinates Reverse (-)

972927 .. 973847

Gene Structure 921 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1002500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 921 bp

ATGGACATCAATATTGATTACGCAAGTAAGCTGTCTCTAAACTCTTCCGGCGGGCAGGATCTTAATCATGATGATGTTAGCAGGGAAGATCCCATCCTCCGACATGAGATCTCCTCCTCCTTTGAGTATTCTTCTCTTTGGCCAAACTTTCCTCTCCATCTTGAAGGGCATCATCAAACCCCATCCTCCTCATCTACCCATCATCAGTATGTTCCCTCCCCTTCGAATATTCATGATCATGATCAACTAATCATCGATCACCATCATCAACACCATCATCGTCATCACACTCATCAAGTACTACTAGCCGCCGCTGCAGGGAATAATAATAATACTTTCATGGAATTAGATGATCACGAAGATGCTCAAGACCAGGGCCCCGCCGCAACTGGTGAAGAAGAGCTTGGAGCTATGAAGGAAATGATGTACAGGATCGCCGCTATGCAGCCGGTGGACATCGATCCAGCTACAATCCGAAAGCCCAAAAGGCGGAATGTGCGAATCAGTGATGATCCCCAGAGCGTGGCTGCGCGTCACCGGCGCGAGAGGATAAGCGAGAAAATTCGTATCCTCCAAAGACTCGTTCCCGGAGGAACTAAAATGGATACCGCTTCTATGTTAGACGAAGCTATTCGATACGTCAAGTTCTTGAAGAGGCAAATCCGATTGCTACAATCATCTTCCTCCTCTTCCAGTAATGTCAATAACATTAGTATCGATGGTCATCATCATCAGCATGATCAGCACCACCCAGCTGCAGCACCCGCAGCAGGAGCTCATGATCAGTGCAATACCAATATTATTAATGGTCCATCAACATCAGTTGGAGTTCCTACTGCAGGGGTTGGGTTGCCGTTGGAATGGCCGCTGCAGCATGGTTCCACCGCCTCCTCCTCCTTCACTAGACACCCTGACAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009889 GO:0009908 GO:0010468 GO:0010500 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048444 GO:0048445 GO:0048449 GO:0048462 GO:0048467 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

307

Amino Acids

34.05

Weight (kDa)

6.21

Isoelectric Point (pI)

56.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	177 - 218	3.5e-07	Helix-loop-helix DNA-binding domain
bHLH_SAC51	PF23173	179 - 222	3.3e-12	Transcription factor SAC51 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00120 AT5G09750
fragaria_vesca	FvH4_1g24110
malus_domestica	MD01G1002500.v1.1
prunus_persica	Prupe.6G159200_v2.0.a1
pyrus_communis	pycom01g02490
rosa_chinensis	RchiOBHm_Chr2g0120331
rosa_laevigata	RLG00000018548
rosa_multiflora	Rmu_co8368037.1_g000001 Rmu_sc0004523.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123560
rosa_rugosa	Rorug02G0228500
rosa_samantha	Rh2AG284800 Rh2BG295800 Rh2CG273400 Rh2DG309800
rosa_wichuraiana	Rw2G022860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	523
AccII	CGCG	2 cut(s)	532, 543
AclWI	GGATC	5 cut(s)	66, 83, 440, 455, 506
AcoI	YGGCCR	2 cut(s)	140, 863
AcsI	RAATTY	1 cut(s)	561
AfaI	GTAC	2 cut(s)	300, 428
AfiI	CCNNNNNNNGG	5 cut(s)	318, 489, 523, 770, 839
AgsI	TTSAA	2 cut(s)	164, 652
AjnI	CCWGG	1 cut(s)	372
AjuI	GAANNNNNNNTTGG	4 cut(s)	387, 419, 567, 599
AluBI	AGCT	7 cut(s)	31, 403, 410, 467, 629, 755, 776
AluI	AGCT	7 cut(s)	31, 403, 410, 467, 629, 755, 776
Alw21I	GWGCWC	1 cut(s)	778
Alw26I	GTCTC	1 cut(s)	39
AlwI	GGATC	5 cut(s)	66, 83, 440, 455, 506
AoxI	GGCC	3 cut(s)	140, 376, 863
ApaI	GGGCCC	1 cut(s)	380
ApeKI	GCWGC	8 cut(s)	314, 445, 527, 755, 758, 767, 868, 871
ApoI	RAATTY	1 cut(s)	561
AseI	ATTAAT	1 cut(s)	804
Asp700I	GAANNNNTTC	1 cut(s)	630
AspLEI	GCGC	2 cut(s)	532, 543
AspS9I	GGNCC	3 cut(s)	376, 377, 809
AsuC2I	CCSGG	1 cut(s)	588
AsuHPI	GGTGA	3 cut(s)	251, 404, 527
AsuII	TTCGAA	1 cut(s)	224
AvaII	GGWCC	1 cut(s)	809
BaeGI	GKGCMC	1 cut(s)	380
BaeI	ACNNNNGTAYC	2 cut(s)	697, 730
BalI	TGGCCA	1 cut(s)	142
BanII	GRGCYC	2 cut(s)	380, 778
Bbv12I	GWGCWC	1 cut(s)	778
BbvI	GCAGC	8 cut(s)	301, 457, 514, 742, 770, 779, 855, 883
BccI	CCATC	8 cut(s)	101, 165, 190, 207, 270, 282, 713, 820
BceAI	ACGGC	1 cut(s)	838
BciT130I	CCWGG	1 cut(s)	374
BciVI	GTATCC	2 cut(s)	578, 598
BclI	TGATCA	5 cut(s)	235, 241, 352, 739, 781
BcnI	CCSGG	1 cut(s)	588
BcoDI	GTCTC	1 cut(s)	39
BfaI	CTAG	3 cut(s)	305, 903, 919
BfmI	CTRYAG	4 cut(s)	315, 756, 837, 869
BfuI	GTATCC	2 cut(s)	578, 598
BglI	GCCNNNNNGGC	1 cut(s)	487
BglII	AGATCT	1 cut(s)	108
BmcAI	AGTACT	1 cut(s)	300
Bme1390I	CCNGG	2 cut(s)	374, 588
Bme18I	GGWCC	1 cut(s)	809
BmgT120I	GGNCC	3 cut(s)	376, 377, 809
BmiI	GGNNCC	3 cut(s)	378, 379, 880
BmrFI	CCNGG	2 cut(s)	374, 588
BmsI	GCATC	2 cut(s)	178, 352
BplI	GAGNNNNNCTC	2 cut(s)	98, 130
Bpu14I	TTCGAA	1 cut(s)	224
BpuEI	CTTGAG	1 cut(s)	351
BpuMI	CCSGG	1 cut(s)	588
Bsa29I	ATCGAT	3 cut(s)	255, 459, 717
BsaBI	GATNNNNATC	4 cut(s)	63, 240, 261, 723
BsaJI	CCNNGG	1 cut(s)	373
Bsc4I	CCNNNNNNNGG	5 cut(s)	318, 489, 523, 770, 839
Bse118I	RCCGGY	2 cut(s)	448, 537
Bse1I	ACTGG	2 cut(s)	394, 693
Bse8I	GATNNNNATC	4 cut(s)	63, 240, 261, 723
BseBI	CCWGG	1 cut(s)	374
BseCI	ATCGAT	3 cut(s)	255, 459, 717
BseDI	CCNNGG	1 cut(s)	373
BseGI	GGATG	2 cut(s)	93, 182
BseJI	GATNNNNATC	4 cut(s)	63, 240, 261, 723
BseLI	CCNNNNNNNGG	5 cut(s)	318, 489, 523, 770, 839
BseNI	ACTGG	2 cut(s)	394, 693
BseRI	GAGGAG	6 cut(s)	103, 106, 178, 676, 880, 883
BseSI	GKGCMC	1 cut(s)	380
BseXI	GCAGC	8 cut(s)	301, 457, 514, 742, 770, 779, 855, 883
BseYI	CCCAGC	1 cut(s)	751
Bsh1236I	CGCG	2 cut(s)	532, 543
BshFI	GGCC	3 cut(s)	142, 378, 865
BshVI	ATCGAT	3 cut(s)	255, 459, 717
BsiHKAI	GWGCWC	1 cut(s)	778
BsiSI	CCGG	4 cut(s)	48, 449, 538, 588
BslI	CCNNNNNNNGG	5 cut(s)	318, 489, 523, 770, 839
BsmAI	GTCTC	1 cut(s)	39
BsnI	GGCC	3 cut(s)	142, 378, 865
Bsp119I	TTCGAA	1 cut(s)	224
Bsp120I	GGGCCC	1 cut(s)	376
Bsp1286I	GDGCHC	2 cut(s)	380, 778
Bsp1407I	TGTACA	1 cut(s)	426
BspANI	GGCC	3 cut(s)	142, 378, 865
BspDI	ATCGAT	3 cut(s)	255, 459, 717
BspFNI	CGCG	2 cut(s)	532, 543
BspHI	TCATGA	4 cut(s)	67, 232, 238, 778
BspLI	GGNNCC	3 cut(s)	378, 379, 880
BspMAI	CTGCAG	4 cut(s)	319, 760, 841, 873
BspPI	GGATC	5 cut(s)	66, 83, 440, 455, 506
BspQI	GCTCTTC	1 cut(s)	393
BspT104I	TTCGAA	1 cut(s)	224
BsrFI	RCCGGY	2 cut(s)	448, 537
BsrGI	TGTACA	1 cut(s)	426
BsrI	ACTGG	2 cut(s)	394, 693
BssAI	RCCGGY	2 cut(s)	448, 537
BssECI	CCNNGG	1 cut(s)	373
Bst2UI	CCWGG	1 cut(s)	374
Bst6I	CTCTTC	4 cut(s)	49, 393, 647, 694
BstAUI	TGTACA	1 cut(s)	426
BstBI	TTCGAA	1 cut(s)	224
BstC8I	GCNNGC	1 cut(s)	53
BstENI	CCTNNNNNAGG	1 cut(s)	837
BstF5I	GGATG	2 cut(s)	93, 182
BstFNI	CGCG	2 cut(s)	532, 543
BstHHI	GCGC	2 cut(s)	532, 543
BstMAI	GTCTC	1 cut(s)	39
BstMWI	GCNNNNNNNGC	9 cut(s)	314, 487, 496, 538, 742, 764, 767, 773, 871
BstNI	CCWGG	1 cut(s)	374
BstSCI	CCNGG	2 cut(s)	372, 586
BstSFI	CTRYAG	4 cut(s)	315, 756, 837, 869
BstSLI	GKGCMC	1 cut(s)	380
BstUI	CGCG	2 cut(s)	532, 543
BstV1I	GCAGC	8 cut(s)	301, 457, 514, 742, 770, 779, 855, 883
BstX2I	RGATCY	3 cut(s)	58, 88, 108
BstYI	RGATCY	3 cut(s)	58, 88, 108
Bsu15I	ATCGAT	3 cut(s)	255, 459, 717
BsuI	GTATCC	2 cut(s)	578, 598
BsuRI	GGCC	3 cut(s)	142, 378, 865
BsuTUI	ATCGAT	3 cut(s)	255, 459, 717
BtsCI	GGATG	2 cut(s)	93, 182
BtsIMutI	CAGTG	2 cut(s)	511, 791
Cac8I	GCNNGC	1 cut(s)	53
CciI	TCATGA	4 cut(s)	67, 232, 238, 778
CfoI	GCGC	2 cut(s)	532, 543
Cfr10I	RCCGGY	2 cut(s)	448, 537
Cfr13I	GGNCC	3 cut(s)	376, 377, 809
ClaI	ATCGAT	3 cut(s)	255, 459, 717
CseI	GACGC	1 cut(s)	521
Csp6I	GTAC	2 cut(s)	299, 427
CviAII	CATG	8 cut(s)	68, 104, 233, 239, 340, 737, 779, 875
CviQI	GTAC	2 cut(s)	299, 427
EaeI	YGGCCR	2 cut(s)	140, 863
Eam1104I	CTCTTC	4 cut(s)	49, 393, 647, 694
EarI	CTCTTC	4 cut(s)	49, 393, 647, 694
EciI	GGCGGA	1 cut(s)	505
Ecl136II	GAGCTC	1 cut(s)	776
Eco24I	GRGCYC	2 cut(s)	380, 778
Eco47I	GGWCC	1 cut(s)	809
Eco53kI	GAGCTC	1 cut(s)	776
EcoICRI	GAGCTC	1 cut(s)	776
EcoNI	CCTNNNNNAGG	1 cut(s)	837
EcoO109I	RGGNCCY	2 cut(s)	376, 377
EcoRII	CCWGG	1 cut(s)	372
EcoT38I	GRGCYC	2 cut(s)	380, 778
FaeI	CATG	8 cut(s)	71, 107, 236, 242, 343, 740, 782, 878
FalI	AAGNNNNNCTT	2 cut(s)	387, 419
FatI	CATG	8 cut(s)	67, 103, 232, 238, 339, 736, 778, 874
FauI	CCCGC	3 cut(s)	44, 388, 772
FbaI	TGATCA	5 cut(s)	235, 241, 352, 739, 781
FokI	GGATG	2 cut(s)	80, 169
FriOI	GRGCYC	2 cut(s)	380, 778
FspBI	CTAG	3 cut(s)	305, 903, 919
GlaI	GCGC	2 cut(s)	531, 542
GsaI	CCCAGC	1 cut(s)	755
HaeIII	GGCC	3 cut(s)	142, 378, 865
HapII	CCGG	4 cut(s)	48, 449, 538, 588
HgaI	GACGC	1 cut(s)	521
HhaI	GCGC	2 cut(s)	532, 543
Hin1II	CATG	8 cut(s)	71, 107, 236, 242, 343, 740, 782, 878
Hin6I	GCGC	2 cut(s)	530, 541
HinP1I	GCGC	2 cut(s)	530, 541
HinfI	GANTC	2 cut(s)	501, 579
HpaII	CCGG	4 cut(s)	48, 449, 538, 588
HphI	GGTGA	3 cut(s)	251, 404, 527
Hpy166II	GTNNAC	1 cut(s)	454
Hpy188I	TCNGA	3 cut(s)	101, 476, 665
Hpy188III	TCNNGA	8 cut(s)	68, 161, 233, 239, 356, 368, 649, 779
Hpy8I	GTNNAC	1 cut(s)	454
HpyAV	CCTTC	4 cut(s)	158, 231, 409, 907
HpyCH4IV	ACGT	1 cut(s)	639
HpyCH4V	TGCA	6 cut(s)	317, 445, 758, 789, 839, 871
HpyF10VI	GCNNNNNNNGC	9 cut(s)	314, 487, 496, 538, 742, 764, 767, 773, 871
HpySE526I	ACGT	1 cut(s)	639
Hsp92II	CATG	8 cut(s)	71, 107, 236, 242, 343, 740, 782, 878
HspAI	GCGC	2 cut(s)	530, 541
Ksp22I	TGATCA	5 cut(s)	235, 241, 352, 739, 781
LguI	GCTCTTC	1 cut(s)	393
LmnI	GCTCC	2 cut(s)	407, 773
Lsp1109I	GCAGC	8 cut(s)	301, 457, 514, 742, 770, 779, 855, 883
LweI	GCATC	2 cut(s)	178, 352
MaeI	CTAG	3 cut(s)	305, 903, 919
MaeII	ACGT	1 cut(s)	639
MaeIII	GTNAC	1 cut(s)	533
MboII	GAAGA	9 cut(s)	36, 98, 123, 371, 407, 410, 664, 672, 681
MflI	RGATCY	3 cut(s)	58, 88, 108
MhlI	GDGCHC	2 cut(s)	380, 778
MlsI	TGGCCA	1 cut(s)	142
MluCI	AATT	2 cut(s)	344, 561
MluNI	TGGCCA	1 cut(s)	142
MlyI	GAGTC	1 cut(s)	573
MmeI	TCCRAC	3 cut(s)	124, 805, 837
Mox20I	TGGCCA	1 cut(s)	142
MroXI	GAANNNNTTC	1 cut(s)	630
MscI	TGGCCA	1 cut(s)	142
MseI	TTAA	2 cut(s)	63, 804
MslI	CAYNNNNRTG	5 cut(s)	72, 207, 237, 360, 735
Msp20I	TGGCCA	1 cut(s)	142
MspA1I	CMGCKG	3 cut(s)	314, 755, 868
MspI	CCGG	4 cut(s)	48, 449, 538, 588
MspR9I	CCNGG	2 cut(s)	374, 588
MvaI	CCWGG	1 cut(s)	374
MvnI	CGCG	2 cut(s)	532, 543
MwoI	GCNNNNNNNGC	9 cut(s)	314, 487, 496, 538, 742, 764, 767, 773, 871
NciI	CCSGG	1 cut(s)	588
NlaIII	CATG	8 cut(s)	71, 107, 236, 242, 343, 740, 782, 878
NlaIV	GGNNCC	3 cut(s)	378, 379, 880
NmuCI	GTSAC	1 cut(s)	533
NspV	TTCGAA	1 cut(s)	224
PagI	TCATGA	4 cut(s)	67, 232, 238, 778
PciSI	GCTCTTC	1 cut(s)	393
PdmI	GAANNNNTTC	1 cut(s)	630
PfeI	GAWTC	1 cut(s)	501
PflMI	CCANNNNNTGG	1 cut(s)	523
PfoI	TCCNGGA	1 cut(s)	586
PleI	GAGTC	1 cut(s)	573
PpsI	GAGTC	1 cut(s)	573
PshBI	ATTAAT	1 cut(s)	804
Psp124BI	GAGCTC	1 cut(s)	778
Psp6I	CCWGG	1 cut(s)	372
PspFI	CCCAGC	1 cut(s)	751
PspGI	CCWGG	1 cut(s)	372
PspN4I	GGNNCC	3 cut(s)	378, 379, 880
PspOMI	GGGCCC	1 cut(s)	376
PspPI	GGNCC	3 cut(s)	376, 377, 809
PstI	CTGCAG	4 cut(s)	319, 760, 841, 873
PsuI	RGATCY	3 cut(s)	58, 88, 108
PvuII	CAGCTG	1 cut(s)	755
RsaI	GTAC	2 cut(s)	300, 428
RsaNI	GTAC	2 cut(s)	299, 427
RseI	CAYNNNNRTG	5 cut(s)	72, 207, 237, 360, 735
SacI	GAGCTC	1 cut(s)	778
SapI	GCTCTTC	1 cut(s)	393
SaqAI	TTAA	2 cut(s)	63, 804
Sau96I	GGNCC	3 cut(s)	376, 377, 809
ScaI	AGTACT	1 cut(s)	300
SchI	GAGTC	1 cut(s)	573
ScrFI	CCNGG	2 cut(s)	374, 588
SduI	GDGCHC	2 cut(s)	380, 778
SetI	ASST	8 cut(s)	33, 405, 412, 469, 631, 642, 757, 778
SfaNI	GCATC	2 cut(s)	178, 352
SfcI	CTRYAG	4 cut(s)	315, 756, 837, 869
SfuI	TTCGAA	1 cut(s)	224
SgrAI	CRCCGGYG	1 cut(s)	537
SinI	GGWCC	1 cut(s)	809
SmiMI	CAYNNNNRTG	5 cut(s)	72, 207, 237, 360, 735
SmlI	CTYRAG	1 cut(s)	366
SmoI	CTYRAG	1 cut(s)	366
Sse9I	AATT	2 cut(s)	344, 561
SspI	AATATT	3 cut(s)	13, 229, 799
SspMI	CTAG	3 cut(s)	305, 903, 919
SstI	GAGCTC	1 cut(s)	778
StyD4I	CCNGG	2 cut(s)	372, 586
TaiI	ACGT	1 cut(s)	642
TaqI	TCGA	5 cut(s)	224, 255, 459, 634, 717
TasI	AATT	2 cut(s)	344, 561
TatI	WGTACW	2 cut(s)	298, 426
TauI	GCSGC	5 cut(s)	311, 314, 386, 440, 868
TfiI	GAWTC	1 cut(s)	501
Tru1I	TTAA	2 cut(s)	63, 804
Tru9I	TTAA	2 cut(s)	63, 804
TscAI	CASTG	2 cut(s)	511, 791
TseFI	GTSAC	1 cut(s)	533
TseI	GCWGC	8 cut(s)	314, 445, 527, 755, 758, 767, 868, 871
Tsp45I	GTSAC	1 cut(s)	533
TspDTI	ATGAA	3 cut(s)	221, 328, 428
TspRI	CASTG	2 cut(s)	511, 791
Van91I	CCANNNNNTGG	1 cut(s)	523
VpaK11BI	GGWCC	1 cut(s)	809
VspI	ATTAAT	1 cut(s)	804
XagI	CCTNNNNNAGG	1 cut(s)	837
XapI	RAATTY	1 cut(s)	561
XmnI	GAANNNNTTC	1 cut(s)	630
XspI	CTAG	3 cut(s)	305, 903, 919
ZrmI	AGTACT	1 cut(s)	300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.