MD01G1005400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013597
Physical Location & Seq
Reverse (-)
1810894 .. 1813553
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1005400.v1.1.491

Sequence Viewer

Length: 690 bp
ATGAGGGCATGTAAAGCACCACCCATTCCCCATCTTCTCCCGACCTCTTCTTCCTCAACTCAACCAGCAAACGTAAAATGGCCATTGCTGAGCAGGCGCTTGGTTCTCTTGTCACTTCCTTTGGCTACTTTTCTGCTCCCAAGTAAAGCTAGCTGTAGTGACACCAACAGTAGCAATGTAAATTCAACAACCCCTTCTGGTTCCACTTCTTCTGCTCTCACAAGCTTCAACCCGATAAGCGCTGCTGAGAGAGACGCAAGCGATGCAATTTCTCGAAGAATATCGGATGCCTTGGAGTTGTTGGAGAAAGGGAGGGAACTGCAGGCTCTGGGTGACTTTGACCAAGCTCTTGTCTGTTTTACTCAGGTGATTGAGAAGTACAATGACTTTTCCTTTTCAGACTATGCAAGAGTAGGCAGATCCTTGGTTTTGTATGAGGTTGGCAACAGAGAAGATGCAGTTGCAGAGATGGAGGATGTTTCCATATCTTTGAAGGGATATCCAGAAGTGCATGCAGCTCTAGCAGCAGCCTTGTATGTCGACAAACACGCCCCACTGCTTGCAGAAAATCAGTTCACAATTGCAACGCTTCTTGATCCTCACTATACGGACCTCTCATACGTCAAAGAAACGAAGCATTGGCCTCCAAGTTTGGTTAGTTCATTGCAGCACTTCATCACACTTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_19

Protein Analysis

230

Amino Acids

24.97

Weight (kDa)

5.49

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013597)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24060 FvH4_1g24440 FvH4_1g24440 FvH4_1g24440
malus_domestica MD01G1005400.v1.1
prunus_persica Prupe.4G271700_v2.0.a1
pyrus_communis pycom01g01890
rosa_chinensis RchiOBHm_Chr2g0121491 RchiOBHm_Chr2g0121541
rosa_laevigata RLG00000018624
rosa_multiflora Rmu_sc0000315.1_g000028
rosa_roxburghii Rroxscaffold_2G00122370
rosa_rugosa Rorug02G0233600
rosa_samantha Rh2AG290400 Rh2BG301200 Rh2CG279900 Rh2DG315100
rosa_wichuraiana Rw2G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 540
AclWI GGATC 2 cut(s) 414, 590
AcoI YGGCCR 1 cut(s) 80
AcsI RAATTY 1 cut(s) 181
AfaI GTAC 1 cut(s) 380
AfeI AGCGCT 1 cut(s) 241
AgsI TTSAA 3 cut(s) 186, 229, 493
AluBI AGCT 5 cut(s) 149, 153, 225, 347, 518
AluI AGCT 5 cut(s) 149, 153, 225, 347, 518
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 2 cut(s) 414, 590
AlwNI CAGNNNCTG 1 cut(s) 328
Aor51HI AGCGCT 1 cut(s) 241
AoxI GGCC 2 cut(s) 80, 641
ApeKI GCWGC 5 cut(s) 242, 515, 524, 527, 667
ApoI RAATTY 1 cut(s) 181
AspLEI GCGC 2 cut(s) 99, 242
AspS9I GGNCC 1 cut(s) 610
AsuHPI GGTGA 2 cut(s) 344, 379
AsuNHI GCTAGC 1 cut(s) 149
AvaII GGWCC 1 cut(s) 610
BalI TGGCCA 1 cut(s) 82
BbvI GCAGC 5 cut(s) 229, 527, 536, 539, 679
BccI CCATC 2 cut(s) 39, 463
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 3 cut(s) 150, 521, 688
BfmI CTRYAG 2 cut(s) 154, 320
BfoI RGCGCY 2 cut(s) 100, 243
BisI GCNGC 5 cut(s) 243, 516, 525, 528, 668
BlpI GCTNAGC 1 cut(s) 89
BlsI GCNGC 5 cut(s) 244, 517, 526, 529, 669
Bme18I GGWCC 1 cut(s) 610
BmgT120I GGNCC 1 cut(s) 610
BmiI GGNNCC 1 cut(s) 202
BmsI GCATC 3 cut(s) 253, 277, 445
BmtI GCTAGC 1 cut(s) 153
Bpu1102I GCTNAGC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 291, 423
Bse3DI GCAATG 3 cut(s) 83, 181, 662
BseDI CCNNGG 2 cut(s) 291, 423
BseGI GGATG 2 cut(s) 292, 481
BseMI GCAATG 3 cut(s) 83, 181, 662
BseMII CTCAG 3 cut(s) 80, 237, 377
BseXI GCAGC 5 cut(s) 229, 527, 536, 539, 679
BshFI GGCC 2 cut(s) 82, 643
BsmAI GTCTC 1 cut(s) 246
BsmBI CGTCTC 1 cut(s) 246
BsnI GGCC 2 cut(s) 82, 643
Bsp143I GATC 2 cut(s) 419, 595
Bsp1720I GCTNAGC 1 cut(s) 89
BspANI GGCC 2 cut(s) 82, 643
BspCNI CTCAG 3 cut(s) 81, 238, 376
BspLI GGNNCC 1 cut(s) 202
BspMAI CTGCAG 1 cut(s) 324
BspOI GCTAGC 1 cut(s) 153
BspPI GGATC 2 cut(s) 414, 590
BsrDI GCAATG 3 cut(s) 83, 181, 662
BssECI CCNNGG 2 cut(s) 291, 423
BssMI GATC 2 cut(s) 419, 595
BssT1I CCWWGG 2 cut(s) 291, 423
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 6 cut(s) 95, 151, 259, 324, 513, 561
BstDEI CTNAG 3 cut(s) 89, 246, 363
BstF5I GGATG 2 cut(s) 292, 481
BstH2I RGCGCY 2 cut(s) 100, 243
BstHHI GCGC 2 cut(s) 99, 242
BstKTI GATC 2 cut(s) 422, 598
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 2 cut(s) 419, 595
BstMWI GCNNNNNNNGC 5 cut(s) 14, 94, 263, 521, 524
BstNSI RCATGY 2 cut(s) 12, 515
BstSFI CTRYAG 2 cut(s) 154, 320
BstV1I GCAGC 5 cut(s) 229, 527, 536, 539, 679
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BsuRI GGCC 2 cut(s) 82, 643
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 2 cut(s) 292, 481
BtsI GCAGTG 1 cut(s) 554
BtsIMutI CAGTG 1 cut(s) 554
Cac8I GCNNGC 6 cut(s) 95, 151, 259, 324, 513, 561
CaiI CAGNNNCTG 1 cut(s) 328
CfoI GCGC 2 cut(s) 99, 242
Cfr13I GGNCC 1 cut(s) 610
CseI GACGC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 379
CviAII CATG 2 cut(s) 9, 512
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 3 cut(s) 89, 246, 363
DpnI GATC 2 cut(s) 421, 597
DpnII GATC 2 cut(s) 419, 595
EaeI YGGCCR 1 cut(s) 80
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 291, 423
Eco32I GATATC 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 610
Eco47III AGCGCT 1 cut(s) 241
EcoRV GATATC 1 cut(s) 500
EcoT14I CCWWGG 2 cut(s) 291, 423
ErhI CCWWGG 2 cut(s) 291, 423
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 2 cut(s) 12, 515
FaiI YATR 8 cut(s) 10, 405, 435, 485, 513, 537, 606, 619
FatI CATG 2 cut(s) 8, 511
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 5 cut(s) 243, 516, 525, 528, 668
FokI GGATG 2 cut(s) 299, 488
Fsp4HI GCNGC 5 cut(s) 243, 516, 525, 528, 668
FspBI CTAG 3 cut(s) 150, 521, 688
GlaI GCGC 2 cut(s) 98, 241
GluI GCNGC 5 cut(s) 243, 516, 525, 528, 668
HaeII RGCGCY 2 cut(s) 100, 243
HaeIII GGCC 2 cut(s) 82, 643
HgaI GACGC 1 cut(s) 263
HhaI GCGC 2 cut(s) 99, 242
Hin1II CATG 2 cut(s) 12, 515
Hin6I GCGC 2 cut(s) 97, 240
HinP1I GCGC 2 cut(s) 97, 240
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 223
HphI GGTGA 2 cut(s) 344, 379
Hpy166II GTNNAC 2 cut(s) 541, 576
Hpy188I TCNGA 2 cut(s) 286, 400
Hpy188III TCNNGA 4 cut(s) 40, 273, 503, 593
Hpy8I GTNNAC 2 cut(s) 541, 576
HpyAV CCTTC 2 cut(s) 204, 487
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 2 cut(s) 72, 621
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 94, 263, 521, 524
HpyF3I CTNAG 3 cut(s) 89, 246, 363
HpySE526I ACGT 2 cut(s) 72, 621
Hsp92II CATG 2 cut(s) 12, 515
HspAI GCGC 2 cut(s) 97, 240
Kzo9I GATC 2 cut(s) 419, 595
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 7 cut(s) 78, 79, 183, 308, 314, 350, 516
Lsp1109I GCAGC 5 cut(s) 229, 527, 536, 539, 679
LweI GCATC 3 cut(s) 253, 277, 445
MaeI CTAG 3 cut(s) 150, 521, 688
MaeII ACGT 2 cut(s) 72, 621
MaeIII GTNAC 3 cut(s) 111, 158, 332
MalI GATC 2 cut(s) 421, 597
MboI GATC 2 cut(s) 419, 595
MboII GAAGA 6 cut(s) 26, 39, 42, 201, 288, 464
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 1 cut(s) 419
MlsI TGGCCA 1 cut(s) 82
MluCI AATT 3 cut(s) 181, 267, 579
MluNI TGGCCA 1 cut(s) 82
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 8 cut(s) 55, 64, 306, 430, 466, 609, 623, 654
Mox20I TGGCCA 1 cut(s) 82
MscI TGGCCA 1 cut(s) 82
Msp20I TGGCCA 1 cut(s) 82
MunI CAATTG 1 cut(s) 579
MwoI GCNNNNNNNGC 5 cut(s) 14, 94, 263, 521, 524
NdeII GATC 2 cut(s) 419, 595
NheI GCTAGC 1 cut(s) 149
NlaIII CATG 2 cut(s) 12, 515
NlaIV GGNNCC 1 cut(s) 202
NmuCI GTSAC 3 cut(s) 111, 158, 332
NspI RCATGY 2 cut(s) 12, 515
PaeI GCATGC 1 cut(s) 515
PkrI GCNGC 5 cut(s) 244, 517, 526, 529, 669
PspN4I GGNNCC 1 cut(s) 202
PspPI GGNCC 1 cut(s) 610
PstI CTGCAG 1 cut(s) 324
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 419
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SalI GTCGAC 1 cut(s) 539
SatI GCNGC 5 cut(s) 243, 516, 525, 528, 668
Sau3AI GATC 2 cut(s) 419, 595
Sau96I GGNCC 1 cut(s) 610
SfaNI GCATC 3 cut(s) 253, 277, 445
SfcI CTRYAG 2 cut(s) 154, 320
SinI GGWCC 1 cut(s) 610
SphI GCATGC 1 cut(s) 515
Sse9I AATT 3 cut(s) 181, 267, 579
SspMI CTAG 3 cut(s) 150, 521, 688
StyI CCWWGG 2 cut(s) 291, 423
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 2 cut(s) 75, 624
TaqI TCGA 2 cut(s) 274, 540
TasI AATT 3 cut(s) 181, 267, 579
TatI WGTACW 1 cut(s) 378
TscAI CASTG 1 cut(s) 561
TseFI GTSAC 3 cut(s) 111, 158, 332
TseI GCWGC 5 cut(s) 242, 515, 524, 527, 667
Tsp45I GTSAC 3 cut(s) 111, 158, 332
TspDTI ATGAA 2 cut(s) 651, 664
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 1 cut(s) 561
VpaK11BI GGWCC 1 cut(s) 610
XapI RAATTY 1 cut(s) 181
XceI RCATGY 2 cut(s) 12, 515
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 3 cut(s) 150, 521, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.