MD01G1010300.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009972
Physical Location & Seq
Forward (+)
5971707 .. 5979770
8064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1010300.v1.1.491

Sequence Viewer

Length: 2310 bp
ATGATTGTGGAACTTGGATTAAAATCTTATACTTGGATTAAAAATCTTGGATTAAAATCTTATACTCTACGAACCAAACCCCATATAAACCCAGACAGAGCAGAGAGAGACGCCGCAAAAATGGGGAAGAAGGAGGAGCAGTTAGCAGAGCTACTCGGAACCCTAGGAGACTTCACCAGCAAAGAGAACTGGGACAAGTTCTTCACCATCCGCGGCACCGACGACTCCTTCGAGTGGTACGCCGAGTGGTCCGAGCTCAGAGATCCTTTGCTCTCCCACCTCCCTCCCCAACCCCAAATTCTCATCCCCGGCTGCGGCAGCTCCCGCCTCTCCGAACACCTCTACGACGCCGGCTTCACTTCCATCACCAACATTGACTTCTCCAAGGTCGCCATTTCCGACTGCCTCCGCCGCAATGTTCGCCACCGCCCCGACATGCGCTGGCGCGTCATGGACATGACCGCCATGCAGTTTTCGGAGGAGGCATTTGATGTTGTAGTTGACAAAGGTGGACTGGATGCCTTAATGGAGCCAGAGATGGGGCCTAAGCTTGGGGATCAGTATTTATCAGAGGTGAGAAGAGTTTTGAAGTGTGGGGGGAAATTTGTTTGCCTTACACTGGCAGAGTCACATGTTCTAGCTTTGCTTTTCTCGAAGTACCGGTTTGGGTGGAAAATGGGTGTTCATGCCATACCTCAGAAACCGTCTAGTAAGCCAAGCCTTCAAGCATATATGGTGGTTGCTGAGAAAGAGGTTTCCAGCGTGTTGCAGGATGTCACAACATCTTTCAATAAGTCTTCTTTTGCTTGTAGGGGAAGTCAGGCTTCTGGACTTCTTGAAGCTGTTGATAAGGAGAACCAAATTCGTAGAGAATACTCCCTTGGTTCGGATCTTAATTTGGGCGGTGGATCCCGTTTCAGTTACAGAGCTGTAGTTCTTGATGCTCAGGATTCATCTGGTCCATTTGCGTATCATTGTGGGGTTTTTATTGTGCCTAAGACTCGGGCTCATGAATGGCTATTCTCCTCTGAAGAAGGACAATGGATGGTAGTAGAAAGCTCTAAGGCAGCTCGTCTAGTAATGATTTTATTAGATGCCAGCCATGTTAGTGCCAGCATGGATGACATTCAGAAGGATTTATCTCCCTTGGTTAAACAATTGGCGCCTGGAAAAGATGACAATGGAGCCCAGATTCCATTCATGATGGCAAGTGATGGGATCAAGCAACGAGATATTGTTCACCAGGTCACATCAACAATAACTGGCCCAATTATAGTTGAGGACGTGATTTATGAAACTGTCGATGGTGATATCAGTCGCATTCTACCATCCAGAGATTTGACGTTTCGTCGCCTTGTTTTCCAAAGAAGTGAGGGTTTGGTTCAGTCTGAAGCTCTTCTATCAGAGGAAGGATCTAACAGTAAAGTTGTTGGTGAGACAGAGAGAAAAAAGACAAATTCATCTTCCAGATCTAAAAGAAAAGGAATCCAGAAAAGAAAAACGAGCCACCAGCTGAAGGTCTATCATGGATATTTGGCTAGTTCTTACCATACAGGGATTATTTCTGGATTAATGTTGATCTCTTCTTATTTGGAAAGCATGGCATCAGCTCAAAAATCAGTTAAAGCAGTTGTAATTGGTCTTGGAGCAGGTTTACTTCCTATGTTCCTTAACAGATGTCTGCCCTTTGTGCACACTGAGGTGGTGGAGTTGGATCCTGTAGTCCTCAAACTTGCAAAAGAATACTTTGGTTTTGTTGAAGATGATTGCTTACAGGCGCACGTTGCTGATGGGATTCAGTTTGTTAGAAATATTGCAAATTCTGCTGCTTCTGATGAAACTTCTCTTGTTCAAGACAAGAAGGATGCTCAATGCAACACTTCTTCCAATGGTGATTTCGAAAGCAGGGTGATTTCGAAGGTTGACATACTTATTATTGATGTGGATTCGGCAGACTCAAGCTCTGGGATGACCTGTCCTGCAGCAGATTTCGTGGACGAATCATTTCTTCAGACTGTAAAGGATGCACTTTCTGAGAAAGGTCTATTTATTATTAATTTGGTATCGCGGTCACAAGCCATTAAAGACACTGTTATTTCGAGGATGAAGGTGGTGTTTAGCCATCTCTTCTCCCTTCAGCTTGAAGAAGACGTCAATGAAGTTATCTTTGGTCTTTGCTCGGCTTCTTGTATCGAGGAGGTTTGCTTCCCTGAAGCCGCTCTTCAACTTGAGAAATTACTAAAACTGGAACACCCGGAGATAAGCCAAAGCATTATAAATACTACGAAGAAGTTGAGACATTTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

770

Amino Acids

85.42

Weight (kDa)

5.91

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_25 PF13649 101 - 200 1.5e-11 Methyltransferase domain
Methyltransf_11 PF08241 103 - 204 4.9e-12 Methyltransferase domain
Methyltransf_31 PF13847 103 - 208 8.8e-09 Methyltransferase domain
Methyltransf_12 PF08242 104 - 202 4.9e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2276
AatII GACGTC 1 cut(s) 2154
Acc36I ACCTGC 1 cut(s) 1640
AccB1I GGYRCC 2 cut(s) 215, 1162
AccBSI CCGCTC 1 cut(s) 2219
AccII CGCG 3 cut(s) 213, 447, 2068
AclWI GGATC 9 cut(s) 257, 564, 897, 903, 916, 1226, 1420, 1709, 1722
AcsI RAATTY 5 cut(s) 297, 602, 861, 1456, 1819
AcuI CTGAAG 6 cut(s) 1050, 1410, 1535, 1994, 2120, 2232
AcyI GRCGYC 4 cut(s) 111, 348, 1163, 2151
AfaI GTAC 2 cut(s) 239, 659
AfiI CCNNNNNNNGG 7 cut(s) 234, 314, 539, 551, 619, 886, 1516
AflIII ACRYGT 1 cut(s) 631
AgeI ACCGGT 1 cut(s) 660
AgsI TTSAA 9 cut(s) 589, 725, 790, 839, 1760, 1853, 2144, 2225, 2305
AhdI GACNNNNNGTC 1 cut(s) 1347
AjiI CACGTC 1 cut(s) 1285
AjnI CCWGG 2 cut(s) 1165, 1242
AleI CACNNNNGTG 1 cut(s) 1700
Alw21I GWGCWC 2 cut(s) 258, 1695
Alw26I GTCTC 4 cut(s) 102, 162, 1430, 2290
Alw44I GTGCAC 1 cut(s) 1691
AlwI GGATC 9 cut(s) 257, 564, 897, 903, 916, 1226, 1420, 1709, 1722
Ama87I CYCGRG 1 cut(s) 1002
AoxI GGCC 2 cut(s) 542, 1264
ApaLI GTGCAC 1 cut(s) 1691
ApeKI GCWGC 5 cut(s) 312, 318, 1067, 1826, 1982
ApoI RAATTY 5 cut(s) 297, 602, 861, 1456, 1819
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AseI ATTAAT 2 cut(s) 1571, 2055
AsiGI ACCGGT 1 cut(s) 660
Asp700I GAANNNNTTC 2 cut(s) 1395, 2004
AspA2I CCTAGG 1 cut(s) 163
AspLEI GCGC 4 cut(s) 441, 447, 1165, 1780
AspS9I GGNCC 4 cut(s) 249, 542, 959, 1265
AsuC2I CCSGG 2 cut(s) 309, 2255
AsuHPI GGTGA 9 cut(s) 166, 196, 358, 586, 1232, 1319, 1445, 1904, 1921
AsuII TTCGAA 2 cut(s) 1899, 1916
AvaI CYCGRG 1 cut(s) 1002
AvaII GGWCC 2 cut(s) 249, 959
AvrII CCTAGG 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 1695
BamHI GGATCC 2 cut(s) 908, 1714
BanI GGYRCC 2 cut(s) 215, 1162
BanII GRGCYC 3 cut(s) 258, 1009, 1189
BbsI GAAGAC 2 cut(s) 789, 2154
Bbv12I GWGCWC 2 cut(s) 258, 1695
BbvI GCAGC 5 cut(s) 299, 330, 1079, 1813, 1994
BciT130I CCWGG 2 cut(s) 1167, 1244
BcnI CCSGG 2 cut(s) 309, 2255
BcoDI GTCTC 4 cut(s) 102, 162, 1430, 2290
BfaI CTAG 5 cut(s) 164, 638, 708, 1076, 1539
BfmI CTRYAG 3 cut(s) 930, 1719, 1980
BfoI RGCGCY 1 cut(s) 1166
BfuAI ACCTGC 1 cut(s) 1640
BglII AGATCT 1 cut(s) 1469
BlnI CCTAGG 1 cut(s) 163
Bme1390I CCNGG 4 cut(s) 309, 1167, 1244, 2255
Bme18I GGWCC 2 cut(s) 249, 959
BmeRI GACNNNNNGTC 1 cut(s) 1347
BmeT110I CYCGRG 1 cut(s) 1002
BmgBI CACGTC 1 cut(s) 1285
BmgT120I GGNCC 4 cut(s) 249, 542, 959, 1265
BmiI GGNNCC 8 cut(s) 160, 217, 531, 543, 910, 1164, 1186, 1716
BmrFI CCNGG 4 cut(s) 309, 1167, 1244, 2255
BmrI ACTGGG 1 cut(s) 199
BmsI GCATC 6 cut(s) 508, 931, 1084, 1613, 1855, 2014
BmuI ACTGGG 1 cut(s) 199
BpiI GAAGAC 2 cut(s) 789, 2154
Bpu10I CCTNAGC 2 cut(s) 546, 945
Bpu14I TTCGAA 2 cut(s) 1899, 1916
BpuEI CTTGAG 2 cut(s) 1942, 2249
BpuMI CCSGG 2 cut(s) 309, 2255
BsaBI GATNNNNATC 2 cut(s) 22, 55
BsaHI GRCGYC 4 cut(s) 111, 348, 1163, 2151
BsaJI CCNNGG 6 cut(s) 163, 211, 307, 384, 880, 1146
BsaWI WCCGGW 1 cut(s) 660
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 7 cut(s) 234, 314, 539, 551, 619, 886, 1516
Bse118I RCCGGY 2 cut(s) 350, 660
Bse1I ACTGG 5 cut(s) 194, 519, 624, 1267, 2250
Bse3DI GCAATG 1 cut(s) 421
Bse8I GATNNNNATC 2 cut(s) 22, 55
BseBI CCWGG 2 cut(s) 1167, 1244
BseDI CCNNGG 6 cut(s) 163, 211, 307, 384, 880, 1146
BseJI GATNNNNATC 2 cut(s) 22, 55
BseLI CCNNNNNNNGG 7 cut(s) 234, 314, 539, 551, 619, 886, 1516
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 6 cut(s) 271, 710, 735, 959, 1689, 2025
BseNI ACTGG 5 cut(s) 194, 519, 624, 1267, 2250
BseRI GAGGAG 4 cut(s) 149, 494, 1015, 2210
BseSI GKGCMC 1 cut(s) 1695
BseXI GCAGC 5 cut(s) 299, 330, 1079, 1813, 1994
Bsh1236I CGCG 3 cut(s) 213, 447, 2068
BshFI GGCC 2 cut(s) 544, 1266
BshNI GGYRCC 2 cut(s) 215, 1162
BshTI ACCGGT 1 cut(s) 660
BsiHKAI GWGCWC 2 cut(s) 258, 1695
BsiHKCI CYCGRG 1 cut(s) 1002
BsiSI CCGG 4 cut(s) 309, 351, 661, 2255
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 7 cut(s) 234, 314, 539, 551, 619, 886, 1516
BsmAI GTCTC 4 cut(s) 102, 162, 1430, 2290
BsmBI CGTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 1320
BsnI GGCC 2 cut(s) 544, 1266
BsoBI CYCGRG 1 cut(s) 1002
Bsp119I TTCGAA 2 cut(s) 1899, 1916
Bsp1286I GDGCHC 4 cut(s) 258, 1009, 1189, 1695
Bsp143I GATC 9 cut(s) 262, 556, 889, 908, 1218, 1412, 1469, 1578, 1714
BspANI GGCC 2 cut(s) 544, 1266
BspCNI CTCAG 6 cut(s) 270, 709, 736, 958, 1690, 2026
BspFNI CGCG 3 cut(s) 213, 447, 2068
BspHI TCATGA 2 cut(s) 1009, 1200
BspLI GGNNCC 8 cut(s) 160, 217, 531, 543, 910, 1164, 1186, 1716
BspMAI CTGCAG 1 cut(s) 1984
BspMI ACCTGC 1 cut(s) 1640
BspPI GGATC 9 cut(s) 257, 564, 897, 903, 916, 1226, 1420, 1709, 1722
BspQI GCTCTTC 2 cut(s) 1401, 2226
BspT104I TTCGAA 2 cut(s) 1899, 1916
BspT107I GGYRCC 2 cut(s) 215, 1162
BsrBI CCGCTC 1 cut(s) 2219
BsrDI GCAATG 1 cut(s) 421
BsrFI RCCGGY 2 cut(s) 350, 660
BsrI ACTGG 5 cut(s) 194, 519, 624, 1267, 2250
BssAI RCCGGY 2 cut(s) 350, 660
BssECI CCNNGG 6 cut(s) 163, 211, 307, 384, 880, 1146
BssMI GATC 9 cut(s) 262, 556, 889, 908, 1218, 1412, 1469, 1578, 1714
BssNI GRCGYC 4 cut(s) 111, 348, 1163, 2151
BssT1I CCWWGG 4 cut(s) 163, 384, 880, 1146
Bst2UI CCWGG 2 cut(s) 1167, 1244
Bst4CI ACNGT 5 cut(s) 705, 1300, 1421, 2017, 2092
Bst6I CTCTTC 5 cut(s) 574, 1401, 1588, 2132, 2226
BstACI GRCGYC 4 cut(s) 111, 348, 1163, 2151
BstAPI GCANNNNNTGC 1 cut(s) 1823
BstBI TTCGAA 2 cut(s) 1899, 1916
BstC8I GCNNGC 4 cut(s) 352, 443, 1099, 1114
BstDEI CTNAG 9 cut(s) 257, 546, 696, 744, 945, 996, 1062, 1698, 2034
BstDSI CCRYGG 1 cut(s) 211
BstFNI CGCG 3 cut(s) 213, 447, 2068
BstH2I RGCGCY 1 cut(s) 1166
BstHHI GCGC 4 cut(s) 441, 447, 1165, 1780
BstKTI GATC 9 cut(s) 265, 559, 892, 911, 1221, 1415, 1472, 1581, 1717
BstMAI GTCTC 4 cut(s) 102, 162, 1430, 2290
BstMBI GATC 9 cut(s) 262, 556, 889, 908, 1218, 1412, 1469, 1578, 1714
BstMWI GCNNNNNNNGC 7 cut(s) 318, 324, 411, 420, 1690, 1784, 1823
BstNI CCWGG 2 cut(s) 1167, 1244
BstNSI RCATGY 2 cut(s) 439, 635
BstSCI CCNGG 4 cut(s) 307, 1165, 1242, 2253
BstSFI CTRYAG 3 cut(s) 930, 1719, 1980
BstSLI GKGCMC 1 cut(s) 1695
BstUI CGCG 3 cut(s) 213, 447, 2068
BstV1I GCAGC 5 cut(s) 299, 330, 1079, 1813, 1994
BstV2I GAAGAC 2 cut(s) 789, 2154
BstX2I RGATCY 6 cut(s) 262, 889, 908, 1412, 1469, 1714
BstYI RGATCY 6 cut(s) 262, 889, 908, 1412, 1469, 1714
BsuRI GGCC 2 cut(s) 544, 1266
BtgI CCRYGG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 1285
BtsIMutI CAGTG 3 cut(s) 617, 1695, 2088
BveI ACCTGC 1 cut(s) 1640
Cac8I GCNNGC 4 cut(s) 352, 443, 1099, 1114
CciI TCATGA 2 cut(s) 1009, 1200
CfoI GCGC 4 cut(s) 441, 447, 1165, 1780
Cfr10I RCCGGY 2 cut(s) 350, 660
Cfr13I GGNCC 4 cut(s) 249, 542, 959, 1265
Cfr42I CCGCGG 1 cut(s) 214
CseI GACGC 3 cut(s) 119, 356, 436
CsiI ACCWGGT 1 cut(s) 1242
Csp6I GTAC 2 cut(s) 238, 658
CspAI ACCGGT 1 cut(s) 660
CviQI GTAC 2 cut(s) 238, 658
DdeI CTNAG 9 cut(s) 257, 546, 696, 744, 945, 996, 1062, 1698, 2034
DinI GGCGCC 1 cut(s) 1164
DpnI GATC 9 cut(s) 264, 558, 891, 910, 1220, 1414, 1471, 1580, 1716
DpnII GATC 9 cut(s) 262, 556, 889, 908, 1218, 1412, 1469, 1578, 1714
DriI GACNNNNNGTC 1 cut(s) 1347
Eam1104I CTCTTC 5 cut(s) 574, 1401, 1588, 2132, 2226
Eam1105I GACNNNNNGTC 1 cut(s) 1347
EarI CTCTTC 5 cut(s) 574, 1401, 1588, 2132, 2226
EciI GGCGGA 1 cut(s) 398
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 4 cut(s) 163, 384, 880, 1146
Eco24I GRGCYC 3 cut(s) 258, 1009, 1189
Eco32I GATATC 1 cut(s) 1312
Eco47I GGWCC 2 cut(s) 249, 959
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 6 cut(s) 1050, 1410, 1535, 1994, 2120, 2232
Eco88I CYCGRG 1 cut(s) 1002
EcoICRI GAGCTC 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 1165, 1242
EcoRV GATATC 1 cut(s) 1312
EcoT14I CCWWGG 4 cut(s) 163, 384, 880, 1146
EcoT38I GRGCYC 3 cut(s) 258, 1009, 1189
EgeI GGCGCC 1 cut(s) 1164
EheI GGCGCC 1 cut(s) 1164
ErhI CCWWGG 4 cut(s) 163, 384, 880, 1146
Esp3I CGTCTC 1 cut(s) 102
FalI AAGNNNNNCTT 4 cut(s) 808, 840, 2205, 2237
FaqI GGGAC 1 cut(s) 206
FauI CCCGC 1 cut(s) 332
FriOI GRGCYC 3 cut(s) 258, 1009, 1189
FspBI CTAG 5 cut(s) 164, 638, 708, 1076, 1539
GlaI GCGC 4 cut(s) 440, 446, 1164, 1779
HaeII RGCGCY 1 cut(s) 1166
HaeIII GGCC 2 cut(s) 544, 1266
HapII CCGG 4 cut(s) 309, 351, 661, 2255
HgaI GACGC 3 cut(s) 119, 356, 436
HhaI GCGC 4 cut(s) 441, 447, 1165, 1780
Hin1I GRCGYC 4 cut(s) 111, 348, 1163, 2151
Hin6I GCGC 4 cut(s) 439, 445, 1163, 1778
HinP1I GCGC 4 cut(s) 439, 445, 1163, 1778
HincII GTYRAC 2 cut(s) 502, 1924
HindII GTYRAC 2 cut(s) 502, 1924
HindIII AAGCTT 1 cut(s) 548
HpaII CCGG 4 cut(s) 309, 351, 661, 2255
HphI GGTGA 9 cut(s) 166, 196, 358, 586, 1232, 1319, 1445, 1904, 1921
Hpy166II GTNNAC 7 cut(s) 502, 512, 1240, 1655, 1693, 1924, 1996
Hpy8I GTNNAC 7 cut(s) 502, 512, 1240, 1655, 1693, 1924, 1996
Hpy99I CGWCG 3 cut(s) 224, 350, 1353
HpyCH4III ACNGT 5 cut(s) 705, 1300, 1421, 2017, 2092
HpyCH4IV ACGT 4 cut(s) 1284, 1343, 1782, 2151
HpyCH4V TGCA 8 cut(s) 469, 769, 1693, 1736, 1817, 1875, 1982, 2027
HpyF10VI GCNNNNNNNGC 7 cut(s) 318, 324, 411, 420, 1690, 1784, 1823
HpyF3I CTNAG 9 cut(s) 257, 546, 696, 744, 945, 996, 1062, 1698, 2034
HpySE526I ACGT 4 cut(s) 1284, 1343, 1782, 2151
Hsp92I GRCGYC 4 cut(s) 111, 348, 1163, 2151
HspAI GCGC 4 cut(s) 439, 445, 1163, 1778
KasI GGCGCC 1 cut(s) 1162
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
KspI CCGCGG 1 cut(s) 214
Kzo9I GATC 9 cut(s) 262, 556, 889, 908, 1218, 1412, 1469, 1578, 1714
LguI GCTCTTC 2 cut(s) 1401, 2226
LmnI GCTCC 5 cut(s) 136, 326, 529, 1184, 1646
Lsp1109I GCAGC 5 cut(s) 299, 330, 1079, 1813, 1994
LweI GCATC 6 cut(s) 508, 931, 1084, 1613, 1855, 2014
MabI ACCWGGT 1 cut(s) 1242
MaeI CTAG 5 cut(s) 164, 638, 708, 1076, 1539
MaeII ACGT 4 cut(s) 1284, 1343, 1782, 2151
MaeIII GTNAC 5 cut(s) 627, 775, 920, 1246, 2070
MalI GATC 9 cut(s) 264, 558, 891, 910, 1220, 1414, 1471, 1580, 1716
MbiI CCGCTC 1 cut(s) 2219
MboI GATC 9 cut(s) 262, 556, 889, 908, 1218, 1412, 1469, 1578, 1714
MfeI CAATTG 1 cut(s) 1157
MflI RGATCY 6 cut(s) 262, 889, 908, 1412, 1469, 1714
MhlI GDGCHC 4 cut(s) 258, 1009, 1189, 1695
Mly113I GGCGCC 1 cut(s) 1163
MlyI GAGTC 4 cut(s) 218, 635, 994, 1949
MmeI TCCRAC 2 cut(s) 423, 1692
MroNI GCCGGC 1 cut(s) 350
MroXI GAANNNNTTC 2 cut(s) 1395, 2004
MslI CAYNNNNRTG 3 cut(s) 455, 1107, 1700
MspA1I CMGCKG 2 cut(s) 213, 1513
MspI CCGG 4 cut(s) 309, 351, 661, 2255
MspR9I CCNGG 4 cut(s) 309, 1167, 1244, 2255
MunI CAATTG 1 cut(s) 1157
Mva1269I GAATGC 1 cut(s) 1320
MvaI CCWGG 2 cut(s) 1167, 1244
MvnI CGCG 3 cut(s) 213, 447, 2068
MwoI GCNNNNNNNGC 7 cut(s) 318, 324, 411, 420, 1690, 1784, 1823
NaeI GCCGGC 1 cut(s) 352
NarI GGCGCC 1 cut(s) 1163
NciI CCSGG 2 cut(s) 309, 2255
NdeII GATC 9 cut(s) 262, 556, 889, 908, 1218, 1412, 1469, 1578, 1714
NgoMIV GCCGGC 1 cut(s) 350
NlaIV GGNNCC 8 cut(s) 160, 217, 531, 543, 910, 1164, 1186, 1716
NmeAIII GCCGAG 2 cut(s) 268, 2159
NmuCI GTSAC 4 cut(s) 627, 775, 1246, 2070
NspI RCATGY 2 cut(s) 439, 635
NspV TTCGAA 2 cut(s) 1899, 1916
OliI CACNNNNGTG 1 cut(s) 1700
PagI TCATGA 2 cut(s) 1009, 1200
PciI ACATGT 1 cut(s) 631
PciSI GCTCTTC 2 cut(s) 1401, 2226
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 396
PctI GAATGC 1 cut(s) 1320
PdiI GCCGGC 1 cut(s) 352
PdmI GAANNNNTTC 2 cut(s) 1395, 2004
PfeI GAWTC 6 cut(s) 950, 1192, 1485, 1795, 1946, 2000
PinAI ACCGGT 1 cut(s) 660
PleI GAGTC 4 cut(s) 218, 634, 994, 1949
PluTI GGCGCC 1 cut(s) 1166
PpsI GAGTC 4 cut(s) 218, 634, 994, 1949
PscI ACATGT 1 cut(s) 631
PshBI ATTAAT 2 cut(s) 1571, 2055
PsiI TTATAA 1 cut(s) 2276
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 1165, 1242
PspGI CCWGG 2 cut(s) 1165, 1242
PspN4I GGNNCC 8 cut(s) 160, 217, 531, 543, 910, 1164, 1186, 1716
PspPI GGNCC 4 cut(s) 249, 542, 959, 1265
PstI CTGCAG 1 cut(s) 1984
PsuI RGATCY 6 cut(s) 262, 889, 908, 1412, 1469, 1714
PvuII CAGCTG 1 cut(s) 1513
RsaI GTAC 2 cut(s) 239, 659
RsaNI GTAC 2 cut(s) 238, 658
RseI CAYNNNNRTG 3 cut(s) 455, 1107, 1700
SacI GAGCTC 1 cut(s) 258
SacII CCGCGG 1 cut(s) 214
SapI GCTCTTC 2 cut(s) 1401, 2226
Sau3AI GATC 9 cut(s) 262, 556, 889, 908, 1218, 1412, 1469, 1578, 1714
Sau96I GGNCC 4 cut(s) 249, 542, 959, 1265
SchI GAGTC 4 cut(s) 218, 635, 994, 1949
ScrFI CCNGG 4 cut(s) 309, 1167, 1244, 2255
SduI GDGCHC 4 cut(s) 258, 1009, 1189, 1695
SexAI ACCWGGT 1 cut(s) 1242
SfaNI GCATC 6 cut(s) 508, 931, 1084, 1613, 1855, 2014
SfcI CTRYAG 3 cut(s) 930, 1719, 1980
SfoI GGCGCC 1 cut(s) 1164
Sfr303I CCGCGG 1 cut(s) 214
SfuI TTCGAA 2 cut(s) 1899, 1916
SgrBI CCGCGG 1 cut(s) 214
SinI GGWCC 2 cut(s) 249, 959
SmiMI CAYNNNNRTG 3 cut(s) 455, 1107, 1700
SmlI CTYRAG 2 cut(s) 1957, 2228
SmoI CTYRAG 2 cut(s) 1957, 2228
SspDI GGCGCC 1 cut(s) 1162
SspI AATATT 1 cut(s) 1813
SspMI CTAG 5 cut(s) 164, 638, 708, 1076, 1539
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 4 cut(s) 307, 1165, 1242, 2253
StyI CCWWGG 4 cut(s) 163, 384, 880, 1146
TaaI ACNGT 5 cut(s) 705, 1300, 1421, 2017, 2092
TaiI ACGT 4 cut(s) 1287, 1346, 1785, 2154
TaqI TCGA 7 cut(s) 231, 653, 1302, 1899, 1916, 2099, 2193
TauI GCSGC 5 cut(s) 116, 216, 318, 414, 2219
TfiI GAWTC 6 cut(s) 950, 1192, 1485, 1795, 1946, 2000
TscAI CASTG 3 cut(s) 624, 1702, 2095
TseFI GTSAC 4 cut(s) 627, 775, 1246, 2070
TseI GCWGC 5 cut(s) 312, 318, 1067, 1826, 1982
Tsp45I GTSAC 4 cut(s) 627, 775, 1246, 2070
TspDTI ATGAA 9 cut(s) 674, 942, 1026, 1189, 1308, 1449, 1851, 2120, 2172
TspRI CASTG 3 cut(s) 624, 1702, 2095
VneI GTGCAC 1 cut(s) 1691
VpaK11BI GGWCC 2 cut(s) 249, 959
VspI ATTAAT 2 cut(s) 1571, 2055
XapI RAATTY 5 cut(s) 297, 602, 861, 1456, 1819
XceI RCATGY 2 cut(s) 439, 635
XmaJI CCTAGG 1 cut(s) 163
XmnI GAANNNNTTC 2 cut(s) 1395, 2004
XspI CTAG 5 cut(s) 164, 638, 708, 1076, 1539
ZraI GACGTC 1 cut(s) 2152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.