Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0009972

Physical Location & Seq

Genomic Coordinates Reverse (-)

38076233 .. 38083742

Gene Structure 7510 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00358100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2196 bp

ATGGCCAAGGAGGAGCAGCTATCGGAGCTTCTGGAAACCCTAGGCGACTTCACAAGCAAAGAGAACTGGGACAAGTTCTTCACCATCCGAGGCACCGAAGACGCCTTCGAGTGGTACGCCGAGTGGTCCGAGCTCCGAGACCCTTTGCTCTCCCACCTCCCGCCAAAACCCCAAATCCTCGTCCCCGGCTGCGGCAGCTCCCGCCTCTCCGAGCACCTCTACGACGCCGGCTTCACCTCCATCACCAACATCGACTTCTCCAAGGTCGCCATTTCCGATTGCCTCCGCCGCAATGTGCGCCTCAGGCCCCTTATGCGGTGGCGCGTCATGGACATGACCGCCATGCAGCTTGAGGATGAGGCATTTGATGCTGTGGTTGACAAAGGTGGATTGGATGCGTTAATGGAGCCGGAGCTTGGCCCTAAGTTAGGGGATCAATATTTAGCTGAGGTTAGAAGAGTTTTGACGTCTGGAGGGAAATTTATTTGCCTTACATTGGCGGAGTCGCATGTTCTGGCTCTGCTTTTCCCGAAGTTCCGGTTTGGGTGGAAAATTAGTGTCCATGCAATACCTCACAAACCGTCTAGTAAGCCGAGTCTTCAAGCATTTATGGTGGTTGCTGTAAAAGAGATGTCCACTGAGTTGCAGGAGATCACATCCTCATTCAATAAGTCTTCCCTTGCTTGTAGAGGAAGTCAGGGTCGTGGACTTCTTGAAGCTGTTGAAAATGAGAATCAAATTCGTAGAGAATACTCCTCTTCTTCAGATATACAGCACTCTCTTGAAGACTTAAAACTTGGAGCTAGAGGATATTTAACTAAGCTTCGCCCGGGTCATCGTTTTCAGCTTACTTTGTGTGGGGGCTCCCACTTCAGTTGCAGAGCTGTAGTTCTTGATGCTAAGGAAAACTCTGGCCCCTTTGTATATCACTGTGGGGTTTTTATTGTGCCTAAGACTCGGGCTCATGAATGGCTGTTCTCTTCTGAAGAAGGACAATGGATGGTAGTAGAAAGCTCAAAGGCAGCTCGTCTAGTAATGGTTTTATTAGATGCCAGCCATGTTAGTGCGAGCATGGATGATATTCAGAGGGAATTATCTCCTTTGGTTAAACAATTGGCGCCTGGAAAAGATGACAATGGAGCTCAAATTCCATTCATGATGGCAAGTGATGGGATCAAACAACGAGATATTGTGCACCAGGTCACATCCTCAATAACTGGACCAATAATAGTTGAGGACGTGATTTATGAAACTGATAATGGTGATATCAGTCGCATTCTACCATCCAGAGATTTGACATTTCGTCGCCTTGTCTTCCAAAGAAGTGAGGGTCTGGTTCAGTCTGAAGCTCTTCTGTCAGAGGAAGGATCGAAATATAAAATTGTTGGTCAGAGTGAGAAGAAAAAAACAAATTCATCTTCCAAATCTAAAAAAGAGGAATCCAGAGACGAAGTGATGATGTTTCATTTTGTTCCTGTAGGATATTTTGAAAGTATACCTACTAAGGTTCAAATTCTTGTCTCAGTCAAAACAGTTGTTATTGGTCTTGGAGCAGGTTTACTTCCTATGTTCCTTCATAAATGTATGCCCTTTATGCACATTGAGGCGGTGGAGTTGGATCCCATGGTCATCAAACTTGCAAAAGAATACTTTGGTTTCATCGAAGACGATCACTTGCAGGTGCACATTGCTGATGGGATTCAGTATCTCAGAAAAGCTGTACATTTTGCTGTGGAGGATGAAAAGTCTGCTGTTCGAGGAAATGAGGATGGTCACTGCAATTCTGACCCCCATCTTCTAATGGAAGCCATTTGGCATTTCATGTCGAAGGCCAGGGAATTTCGAAGTTCTGGAATGACCTGCCCTGCAGCTAATTTTGTGGACGAGTCGTTTCTTCGGACTGTGAAGGATGCCCTTACTGAGAAGGGTCTATTTATTATAAATTTGGTTTCACGGTCCCAAGACATTAAAGGCACTGTTATTTCAAGGATGAAAATGGTGTTTAGCCATCTCTTCTGCCTTCAGCTTGAAGAAGATGTCAATGAAGTTATTTTTGCCCTTCCTTCAGCTTCCTGTATCAAGGAGGATGACTTCGCCAAAGCCAGTCTTCAACTTGAGAAATTGTTAAAATTGGAGCACCCGGAGATAAGCCAAAGCATTATAGATGCTACAAAGAAGATAAGACATCTGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

731

Amino Acids

82.21

Weight (kDa)

5.98

Isoelectric Point (pI)

48.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_25	PF13649	59 - 159	1.9e-10	Methyltransferase domain
Methyltransf_31	PF13847	60 - 167	3.6e-07	Methyltransferase domain
Methyltransf_11	PF08241	62 - 163	2.3e-12	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31740 AT2G31740
fragaria_vesca	FvH4_4g13510
malus_domestica	MD01G1010300.v1.1 MD04G1013700.v1.1
prunus_persica	Prupe.1G159900_v2.0.a1 Prupe.1G159900_v2.0.a1 Prupe.1G159900_v2.0.a1
pyrus_communis	pycom04g01070
rosa_chinensis	RchiOBHm_Chr4g0414141
rosa_laevigata	RLG00000008167
rosa_multiflora	Rmu_sc0000808.1_g000057 Rmu_sc0008865.1_g000003
rosa_roxburghii	Rroxscaffold_5G00358100
rosa_rugosa	Rorug04G0126400 Rorug04G0126500
rosa_samantha	Rh4AG184600 Rh4BG184000 Rh4CG196300 Rh4DG179900
rosa_wichuraiana	Rw4G015720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1940
AarI	CACCTGC	1 cut(s)	1669
AatII	GACGTC	1 cut(s)	470
Acc36I	ACCTGC	3 cut(s)	1544, 1669, 1868
AccB1I	GGYRCC	2 cut(s)	92, 1117
AccI	GTMKAC	1 cut(s)	1495
AccII	CGCG	1 cut(s)	324
AciI	CCGC	9 cut(s)	161, 192, 202, 286, 289, 316, 339, 500, 1607
AclWI	GGATC	5 cut(s)	441, 1181, 1375, 1613, 1626
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	7 cut(s)	479, 738, 1146, 1411, 1512, 1838, 1942
AcuI	CTGAAG	6 cut(s)	747, 856, 1005, 1365, 2006, 2049
AcyI	GRCGYC	4 cut(s)	102, 225, 467, 1118
AfaI	GTAC	2 cut(s)	116, 1722
AfiI	CCNNNNNNNGG	6 cut(s)	111, 191, 315, 416, 428, 496
AhdI	GACNNNNNGTC	1 cut(s)	1302
AjiI	CACGTC	1 cut(s)	1240
AjnI	CCWGG	3 cut(s)	1120, 1197, 1832
AjuI	GAANNNNNNNTTGG	2 cut(s)	239, 271
Alw21I	GWGCWC	6 cut(s)	135, 216, 1144, 1197, 1686, 2139
Alw26I	GTCTC	3 cut(s)	132, 1440, 1525
Alw44I	GTGCAC	2 cut(s)	1193, 1682
AlwI	GGATC	5 cut(s)	441, 1181, 1375, 1613, 1626
Ama87I	CYCGRG	2 cut(s)	829, 957
AoxI	GGCC	5 cut(s)	3, 305, 418, 913, 1830
ApaLI	GTGCAC	2 cut(s)	1193, 1682
ApeKI	GCWGC	6 cut(s)	16, 189, 195, 346, 1022, 1868
ApoI	RAATTY	7 cut(s)	479, 738, 1146, 1411, 1512, 1838, 1942
Asp700I	GAANNNNTTC	1 cut(s)	1350
AspA2I	CCTAGG	1 cut(s)	40
AspLEI	GCGC	3 cut(s)	300, 324, 1120
AspS9I	GGNCC	6 cut(s)	126, 306, 419, 914, 1220, 1956
AsuC2I	CCSGG	4 cut(s)	186, 830, 831, 2141
AsuHPI	GGTGA	4 cut(s)	73, 226, 235, 1274
AsuII	TTCGAA	1 cut(s)	1843
AvaI	CYCGRG	2 cut(s)	829, 957
AvaII	GGWCC	3 cut(s)	126, 1220, 1956
AvrII	CCTAGG	1 cut(s)	40
AxyI	CCTNAGG	1 cut(s)	302
BaeGI	GKGCMC	2 cut(s)	1197, 1686
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	1618
BanI	GGYRCC	2 cut(s)	92, 1117
BanII	GRGCYC	4 cut(s)	135, 866, 964, 1144
BbsI	GAAGAC	7 cut(s)	105, 590, 666, 792, 1306, 1671, 2099
Bbv12I	GWGCWC	6 cut(s)	135, 216, 1144, 1197, 1686, 2139
BbvCI	CCTCAGC	1 cut(s)	447
BbvI	GCAGC	6 cut(s)	28, 176, 207, 358, 1034, 1880
BciT130I	CCWGG	3 cut(s)	1122, 1199, 1834
BcnI	CCSGG	4 cut(s)	186, 830, 831, 2141
BcoDI	GTCTC	3 cut(s)	132, 1440, 1525
BfaI	CTAG	4 cut(s)	41, 585, 804, 1031
BfmI	CTRYAG	3 cut(s)	885, 1476, 1866
BfoI	RGCGCY	1 cut(s)	1121
BfuAI	ACCTGC	3 cut(s)	1544, 1669, 1868
BisI	GCNGC	8 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1869
BlnI	CCTAGG	1 cut(s)	40
BlsI	GCNGC	8 cut(s)	18, 191, 194, 197, 290, 348, 1024, 1870
Bme1390I	CCNGG	7 cut(s)	186, 830, 831, 1122, 1199, 1834, 2141
Bme18I	GGWCC	3 cut(s)	126, 1220, 1956
BmeRI	GACNNNNNGTC	1 cut(s)	1302
BmeT110I	CYCGRG	2 cut(s)	829, 957
BmgBI	CACGTC	1 cut(s)	1240
BmgT120I	GGNCC	6 cut(s)	126, 306, 419, 914, 1220, 1956
BmiI	GGNNCC	8 cut(s)	94, 308, 408, 865, 916, 1119, 1620, 1958
BmrFI	CCNGG	7 cut(s)	186, 830, 831, 1122, 1199, 1834, 2141
BmrI	ACTGGG	1 cut(s)	76
BmsI	GCATC	6 cut(s)	358, 385, 886, 1039, 1900, 2155
BmuI	ACTGGG	1 cut(s)	76
BpiI	GAAGAC	7 cut(s)	105, 590, 666, 792, 1306, 1671, 2099
BpmI	CTGGAG	1 cut(s)	492
Bpu10I	CCTNAGC	2 cut(s)	447, 900
Bpu14I	TTCGAA	1 cut(s)	1843
BpuEI	CTTGAG	2 cut(s)	371, 2135
BpuMI	CCSGG	4 cut(s)	186, 830, 831, 2141
BsaHI	GRCGYC	4 cut(s)	102, 225, 467, 1118
BsaI	GGTCTC	1 cut(s)	132
BsaJI	CCNNGG	8 cut(s)	6, 40, 88, 184, 261, 829, 1623, 1833
BsaWI	WCCGGW	1 cut(s)	537
Bsc4I	CCNNNNNNNGG	6 cut(s)	111, 191, 315, 416, 428, 496
Bse118I	RCCGGY	1 cut(s)	227
Bse1I	ACTGG	3 cut(s)	71, 1222, 2103
Bse21I	CCTNAGG	1 cut(s)	302
Bse3DI	GCAATG	2 cut(s)	298, 1686
BseBI	CCWGG	3 cut(s)	1122, 1199, 1834
BseDI	CCNNGG	8 cut(s)	6, 40, 88, 184, 261, 829, 1623, 1833
BseLI	CCNNNNNNNGG	6 cut(s)	111, 191, 315, 416, 428, 496
BseMI	GCAATG	2 cut(s)	298, 1686
BseMII	CTCAG	6 cut(s)	316, 438, 630, 1536, 1723, 1911
BseNI	ACTGG	3 cut(s)	71, 1222, 2103
BseRI	GAGGAG	2 cut(s)	26, 745
BseSI	GKGCMC	2 cut(s)	1197, 1686
BseXI	GCAGC	6 cut(s)	28, 176, 207, 358, 1034, 1880
Bsh1236I	CGCG	1 cut(s)	324
BshFI	GGCC	5 cut(s)	5, 307, 420, 915, 1832
BshNI	GGYRCC	2 cut(s)	92, 1117
BsiHKAI	GWGCWC	6 cut(s)	135, 216, 1144, 1197, 1686, 2139
BsiHKCI	CYCGRG	2 cut(s)	829, 957
BsiSI	CCGG	6 cut(s)	186, 228, 410, 538, 830, 2141
BslFI	GGGAC	3 cut(s)	83, 167, 1942
BslI	CCNNNNNNNGG	6 cut(s)	111, 191, 315, 416, 428, 496
BsmAI	GTCTC	3 cut(s)	132, 1440, 1525
BsmBI	CGTCTC	1 cut(s)	1440
BsmFI	GGGAC	3 cut(s)	83, 167, 1942
BsmI	GAATGC	1 cut(s)	1275
BsnI	GGCC	5 cut(s)	5, 307, 420, 915, 1832
Bso31I	GGTCTC	1 cut(s)	132
BsoBI	CYCGRG	2 cut(s)	829, 957
Bsp119I	TTCGAA	1 cut(s)	1843
Bsp1286I	GDGCHC	8 cut(s)	135, 216, 866, 964, 1144, 1197, 1686, 2139
Bsp1407I	TGTACA	1 cut(s)	1720
Bsp143I	GATC	6 cut(s)	433, 651, 1173, 1367, 1618, 1669
Bsp19I	CCATGG	1 cut(s)	1623
BspACI	CCGC	9 cut(s)	161, 192, 202, 286, 289, 316, 339, 500, 1607
BspANI	GGCC	5 cut(s)	5, 307, 420, 915, 1832
BspCNI	CTCAG	6 cut(s)	315, 439, 631, 1535, 1722, 1912
BspFNI	CGCG	1 cut(s)	324
BspHI	TCATGA	2 cut(s)	964, 1155
BspLI	GGNNCC	8 cut(s)	94, 308, 408, 865, 916, 1119, 1620, 1958
BspMAI	CTGCAG	1 cut(s)	1870
BspMI	ACCTGC	3 cut(s)	1544, 1669, 1868
BspPI	GGATC	5 cut(s)	441, 1181, 1375, 1613, 1626
BspQI	GCTCTTC	1 cut(s)	1356
BspT104I	TTCGAA	1 cut(s)	1843
BspT107I	GGYRCC	2 cut(s)	92, 1117
BspTNI	GGTCTC	1 cut(s)	132
BsrDI	GCAATG	2 cut(s)	298, 1686
BsrFI	RCCGGY	1 cut(s)	227
BsrGI	TGTACA	1 cut(s)	1720
BsrI	ACTGG	3 cut(s)	71, 1222, 2103
BssAI	RCCGGY	1 cut(s)	227
BssECI	CCNNGG	8 cut(s)	6, 40, 88, 184, 261, 829, 1623, 1833
BssMI	GATC	6 cut(s)	433, 651, 1173, 1367, 1618, 1669
BssNAI	GTATAC	1 cut(s)	1496
BssNI	GRCGYC	4 cut(s)	102, 225, 467, 1118
BssT1I	CCWWGG	4 cut(s)	6, 40, 261, 1623
Bst1107I	GTATAC	1 cut(s)	1496
Bst2UI	CCWGG	3 cut(s)	1122, 1199, 1834
Bst4CI	ACNGT	6 cut(s)	582, 932, 1534, 1903, 1956, 1978
Bst6I	CTCTTC	5 cut(s)	451, 763, 985, 1356, 2018
BstACI	GRCGYC	4 cut(s)	102, 225, 467, 1118
BstAPI	GCANNNNNTGC	1 cut(s)	368
BstAUI	TGTACA	1 cut(s)	1720
BstBI	TTCGAA	1 cut(s)	1843
BstC8I	GCNNGC	3 cut(s)	229, 1054, 1069
BstDSI	CCRYGG	1 cut(s)	1623
BstENI	CCTNNNNNAGG	1 cut(s)	426
BstFNI	CGCG	1 cut(s)	324
BstH2I	RGCGCY	1 cut(s)	1121
BstHHI	GCGC	3 cut(s)	300, 324, 1120
BstKTI	GATC	6 cut(s)	436, 654, 1176, 1370, 1621, 1672
BstMAI	GTCTC	3 cut(s)	132, 1440, 1525
BstMBI	GATC	6 cut(s)	433, 651, 1173, 1367, 1618, 1669
BstMWI	GCNNNNNNNGC	9 cut(s)	25, 195, 201, 288, 297, 304, 313, 368, 1594
BstNI	CCWGG	3 cut(s)	1122, 1199, 1834
BstNSI	RCATGY	1 cut(s)	512
BstSCI	CCNGG	7 cut(s)	184, 828, 829, 1120, 1197, 1832, 2139
BstSFI	CTRYAG	3 cut(s)	885, 1476, 1866
BstSLI	GKGCMC	2 cut(s)	1197, 1686
BstUI	CGCG	1 cut(s)	324
BstV1I	GCAGC	6 cut(s)	28, 176, 207, 358, 1034, 1880
BstV2I	GAAGAC	7 cut(s)	105, 590, 666, 792, 1306, 1671, 2099
BstX2I	RGATCY	1 cut(s)	1618
BstYI	RGATCY	1 cut(s)	1618
BstZ17I	GTATAC	1 cut(s)	1496
Bsu36I	CCTNAGG	1 cut(s)	302
BsuRI	GGCC	5 cut(s)	5, 307, 420, 915, 1832
BtgI	CCRYGG	1 cut(s)	1623
BtrI	CACGTC	1 cut(s)	1240
BtsI	GCAGTG	1 cut(s)	1774
BtsIMutI	CAGTG	4 cut(s)	636, 928, 1774, 1974
BveI	ACCTGC	3 cut(s)	1544, 1669, 1868
Cac8I	GCNNGC	3 cut(s)	229, 1054, 1069
CciI	TCATGA	2 cut(s)	964, 1155
CfoI	GCGC	3 cut(s)	300, 324, 1120
Cfr10I	RCCGGY	1 cut(s)	227
Cfr13I	GGNCC	6 cut(s)	126, 306, 419, 914, 1220, 1956
Cfr9I	CCCGGG	1 cut(s)	829
CseI	GACGC	3 cut(s)	110, 233, 313
CsiI	ACCWGGT	1 cut(s)	1197
Csp6I	GTAC	2 cut(s)	115, 1721
CspCI	CAANNNNNGTGG	2 cut(s)	857, 892
CviQI	GTAC	2 cut(s)	115, 1721
DinI	GGCGCC	1 cut(s)	1119
DpnI	GATC	6 cut(s)	435, 653, 1175, 1369, 1620, 1671
DpnII	GATC	6 cut(s)	433, 651, 1173, 1367, 1618, 1669
DriI	GACNNNNNGTC	1 cut(s)	1302
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	5 cut(s)	451, 763, 985, 1356, 2018
Eam1105I	GACNNNNNGTC	1 cut(s)	1302
EarI	CTCTTC	5 cut(s)	451, 763, 985, 1356, 2018
EciI	GGCGGA	2 cut(s)	275, 515
Ecl136II	GAGCTC	2 cut(s)	133, 1142
Eco130I	CCWWGG	4 cut(s)	6, 40, 261, 1623
Eco24I	GRGCYC	4 cut(s)	135, 866, 964, 1144
Eco31I	GGTCTC	1 cut(s)	132
Eco32I	GATATC	1 cut(s)	1267
Eco47I	GGWCC	3 cut(s)	126, 1220, 1956
Eco53kI	GAGCTC	2 cut(s)	133, 1142
Eco57I	CTGAAG	6 cut(s)	747, 856, 1005, 1365, 2006, 2049
Eco81I	CCTNAGG	1 cut(s)	302
Eco88I	CYCGRG	2 cut(s)	829, 957
EcoICRI	GAGCTC	2 cut(s)	133, 1142
EcoNI	CCTNNNNNAGG	1 cut(s)	426
EcoO109I	RGGNCCY	1 cut(s)	306
EcoRII	CCWGG	3 cut(s)	1120, 1197, 1832
EcoRV	GATATC	1 cut(s)	1267
EcoT14I	CCWWGG	4 cut(s)	6, 40, 261, 1623
EcoT38I	GRGCYC	4 cut(s)	135, 866, 964, 1144
EgeI	GGCGCC	1 cut(s)	1119
EheI	GGCGCC	1 cut(s)	1119
ErhI	CCWWGG	4 cut(s)	6, 40, 261, 1623
Esp3I	CGTCTC	1 cut(s)	1440
FalI	AAGNNNNNCTT	2 cut(s)	2091, 2123
FaqI	GGGAC	3 cut(s)	83, 167, 1942
FauI	CCCGC	2 cut(s)	168, 209
FblI	GTMKAC	1 cut(s)	1495
Fnu4HI	GCNGC	8 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1869
FriOI	GRGCYC	4 cut(s)	135, 866, 964, 1144
Fsp4HI	GCNGC	8 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1869
FspBI	CTAG	4 cut(s)	41, 585, 804, 1031
GlaI	GCGC	3 cut(s)	299, 323, 1119
GluI	GCNGC	8 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1869
GsuI	CTGGAG	1 cut(s)	492
HaeII	RGCGCY	1 cut(s)	1121
HaeIII	GGCC	5 cut(s)	5, 307, 420, 915, 1832
HapII	CCGG	6 cut(s)	186, 228, 410, 538, 830, 2141
HgaI	GACGC	3 cut(s)	110, 233, 313
HhaI	GCGC	3 cut(s)	300, 324, 1120
Hin1I	GRCGYC	4 cut(s)	102, 225, 467, 1118
Hin6I	GCGC	3 cut(s)	298, 322, 1118
HinP1I	GCGC	3 cut(s)	298, 322, 1118
HincII	GTYRAC	1 cut(s)	379
HindII	GTYRAC	1 cut(s)	379
HindIII	AAGCTT	1 cut(s)	821
HinfI	GANTC	7 cut(s)	503, 595, 733, 955, 1439, 1699, 1886
HpaII	CCGG	6 cut(s)	186, 228, 410, 538, 830, 2141
HphI	GGTGA	4 cut(s)	73, 226, 235, 1274
Hpy166II	GTNNAC	8 cut(s)	379, 636, 707, 1195, 1496, 1559, 1684, 1882
Hpy8I	GTNNAC	8 cut(s)	379, 636, 707, 1195, 1496, 1559, 1684, 1882
Hpy99I	CGWCG	2 cut(s)	227, 1308
HpyCH4III	ACNGT	6 cut(s)	582, 932, 1534, 1903, 1956, 1978
HpyCH4IV	ACGT	2 cut(s)	467, 1239
HpyF10VI	GCNNNNNNNGC	9 cut(s)	25, 195, 201, 288, 297, 304, 313, 368, 1594
HpySE526I	ACGT	2 cut(s)	467, 1239
Hsp92I	GRCGYC	4 cut(s)	102, 225, 467, 1118
HspAI	GCGC	3 cut(s)	298, 322, 1118
KasI	GGCGCC	1 cut(s)	1117
KroI	GCCGGC	1 cut(s)	227
KroNI	GCCGGC	1 cut(s)	229
Kzo9I	GATC	6 cut(s)	433, 651, 1173, 1367, 1618, 1669
LguI	GCTCTTC	1 cut(s)	1356
Lsp1109I	GCAGC	6 cut(s)	28, 176, 207, 358, 1034, 1880
LweI	GCATC	6 cut(s)	358, 385, 886, 1039, 1900, 2155
MabI	ACCWGGT	1 cut(s)	1197
MaeI	CTAG	4 cut(s)	41, 585, 804, 1031
MaeII	ACGT	2 cut(s)	467, 1239
MaeIII	GTNAC	2 cut(s)	1201, 1772
MalI	GATC	6 cut(s)	435, 653, 1175, 1369, 1620, 1671
MboI	GATC	6 cut(s)	433, 651, 1173, 1367, 1618, 1669
MfeI	CAATTG	1 cut(s)	1112
MflI	RGATCY	1 cut(s)	1618
MhlI	GDGCHC	8 cut(s)	135, 216, 866, 964, 1144, 1197, 1686, 2139
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	1118
MlyI	GAGTC	4 cut(s)	512, 604, 949, 1895
MmeI	TCCRAC	1 cut(s)	1596
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	1 cut(s)	227
MroXI	GAANNNNTTC	1 cut(s)	1350
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	6 cut(s)	401, 791, 815, 1107, 1968, 2126
MslI	CAYNNNNRTG	2 cut(s)	332, 1062
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	6 cut(s)	186, 228, 410, 538, 830, 2141
MspR9I	CCNGG	7 cut(s)	186, 830, 831, 1122, 1199, 1834, 2141
MunI	CAATTG	1 cut(s)	1112
Mva1269I	GAATGC	1 cut(s)	1275
MvaI	CCWGG	3 cut(s)	1122, 1199, 1834
MvnI	CGCG	1 cut(s)	324
MwoI	GCNNNNNNNGC	9 cut(s)	25, 195, 201, 288, 297, 304, 313, 368, 1594
NaeI	GCCGGC	1 cut(s)	229
NarI	GGCGCC	1 cut(s)	1118
NciI	CCSGG	4 cut(s)	186, 830, 831, 2141
NcoI	CCATGG	1 cut(s)	1623
NdeII	GATC	6 cut(s)	433, 651, 1173, 1367, 1618, 1669
NgoMIV	GCCGGC	1 cut(s)	227
NlaIV	GGNNCC	8 cut(s)	94, 308, 408, 865, 916, 1119, 1620, 1958
NmeAIII	GCCGAG	2 cut(s)	145, 618
NmuCI	GTSAC	2 cut(s)	1201, 1772
NspI	RCATGY	1 cut(s)	512
NspV	TTCGAA	1 cut(s)	1843
PagI	TCATGA	2 cut(s)	964, 1155
PaqCI	CACCTGC	1 cut(s)	1669
PciSI	GCTCTTC	1 cut(s)	1356
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PctI	GAATGC	1 cut(s)	1275
PdiI	GCCGGC	1 cut(s)	229
PdmI	GAANNNNTTC	1 cut(s)	1350
PfeI	GAWTC	3 cut(s)	733, 1439, 1699
PkrI	GCNGC	8 cut(s)	18, 191, 194, 197, 290, 348, 1024, 1870
PleI	GAGTC	4 cut(s)	511, 603, 949, 1894
PluTI	GGCGCC	1 cut(s)	1121
PpsI	GAGTC	4 cut(s)	511, 603, 949, 1894
PsiI	TTATAA	1 cut(s)	1940
Psp124BI	GAGCTC	2 cut(s)	135, 1144
Psp6I	CCWGG	3 cut(s)	1120, 1197, 1832
PspGI	CCWGG	3 cut(s)	1120, 1197, 1832
PspN4I	GGNNCC	8 cut(s)	94, 308, 408, 865, 916, 1119, 1620, 1958
PspPI	GGNCC	6 cut(s)	126, 306, 419, 914, 1220, 1956
PstI	CTGCAG	1 cut(s)	1870
PsuI	RGATCY	1 cut(s)	1618
RsaI	GTAC	2 cut(s)	116, 1722
RsaNI	GTAC	2 cut(s)	115, 1721
RseI	CAYNNNNRTG	2 cut(s)	332, 1062
SacI	GAGCTC	2 cut(s)	135, 1144
SapI	GCTCTTC	1 cut(s)	1356
SaqAI	TTAA	6 cut(s)	401, 791, 815, 1107, 1968, 2126
SatI	GCNGC	8 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1869
Sau3AI	GATC	6 cut(s)	433, 651, 1173, 1367, 1618, 1669
Sau96I	GGNCC	6 cut(s)	126, 306, 419, 914, 1220, 1956
SchI	GAGTC	4 cut(s)	512, 604, 949, 1895
ScrFI	CCNGG	7 cut(s)	186, 830, 831, 1122, 1199, 1834, 2141
SduI	GDGCHC	8 cut(s)	135, 216, 866, 964, 1144, 1197, 1686, 2139
SexAI	ACCWGGT	1 cut(s)	1197
SfaNI	GCATC	6 cut(s)	358, 385, 886, 1039, 1900, 2155
SfcI	CTRYAG	3 cut(s)	885, 1476, 1866
SfoI	GGCGCC	1 cut(s)	1119
SfuI	TTCGAA	1 cut(s)	1843
SinI	GGWCC	3 cut(s)	126, 1220, 1956
SmaI	CCCGGG	1 cut(s)	831
SmiMI	CAYNNNNRTG	2 cut(s)	332, 1062
SmlI	CTYRAG	2 cut(s)	350, 2114
SmoI	CTYRAG	2 cut(s)	350, 2114
SsiI	CCGC	9 cut(s)	161, 192, 202, 286, 289, 316, 339, 500, 1607
SspDI	GGCGCC	1 cut(s)	1117
SspI	AATATT	1 cut(s)	440
SspMI	CTAG	4 cut(s)	41, 585, 804, 1031
SstI	GAGCTC	2 cut(s)	135, 1144
StyD4I	CCNGG	7 cut(s)	184, 828, 829, 1120, 1197, 1832, 2139
StyI	CCWWGG	4 cut(s)	6, 40, 261, 1623
TaaI	ACNGT	6 cut(s)	582, 932, 1534, 1903, 1956, 1978
TaiI	ACGT	2 cut(s)	470, 1242
TaqI	TCGA	7 cut(s)	108, 252, 1370, 1662, 1756, 1826, 1843
TatI	WGTACW	1 cut(s)	1720
TauI	GCSGC	2 cut(s)	195, 291
TfiI	GAWTC	3 cut(s)	733, 1439, 1699
Tru1I	TTAA	6 cut(s)	401, 791, 815, 1107, 1968, 2126
Tru9I	TTAA	6 cut(s)	401, 791, 815, 1107, 1968, 2126
TscAI	CASTG	4 cut(s)	643, 935, 1781, 1981
TseFI	GTSAC	2 cut(s)	1201, 1772
TseI	GCWGC	6 cut(s)	16, 189, 195, 346, 1022, 1868
Tsp45I	GTSAC	2 cut(s)	1201, 1772
TspMI	CCCGGG	1 cut(s)	829
TspRI	CASTG	4 cut(s)	643, 935, 1781, 1981
VneI	GTGCAC	2 cut(s)	1193, 1682
VpaK11BI	GGWCC	3 cut(s)	126, 1220, 1956
XagI	CCTNNNNNAGG	1 cut(s)	426
XapI	RAATTY	7 cut(s)	479, 738, 1146, 1411, 1512, 1838, 1942
XceI	RCATGY	1 cut(s)	512
XmaI	CCCGGG	1 cut(s)	829
XmaJI	CCTAGG	1 cut(s)	40
XmiI	GTMKAC	1 cut(s)	1495
XmnI	GAANNNNTTC	1 cut(s)	1350
XspI	CTAG	4 cut(s)	41, 585, 804, 1031
ZraI	GACGTC	1 cut(s)	468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.