Rorug04G0126400

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009972
Physical Location & Seq
Reverse (-)
20079490 .. 20080091
602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0126400.1

Sequence Viewer

Length: 384 bp
ATGTCGGACGGGCTGGAACAAAGCGACGATGAAATTGAAACGGATGACTCCATGGCCGACGCAGAGCCGCCGGCGATTCGCAACGAGGAATTCCGGTGGCAGAGAGTGGAGAAGCTCTGCGAAGATGTGAAACAGTTCGGCGAGGAAATGATTGACGACGGCGCTCTCGCCTCTATCTACGATTTCCGAATCGATAAATTTCAGAGATTAGCTATACAAGCATTCTTGAGAGATTCATCAGTAGTTGTGTCTGCGCCGACGAGTAGCGGAAAGACTTTGATAGCAGAAGCTGCTGCTCTAGCAACCGTAGCCAAGGGAAGGAGAATGTTCTACACAACTCCGCTTAAGGCCTTATCGAATCAGAAGTTTCGCGAATTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

127

Amino Acids

14.34

Weight (kDa)

4.66

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 66 - 127 1.6e-12 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 372
AciI CCGC 3 cut(s) 68, 267, 341
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 3 cut(s) 89, 197, 374
AfiI CCNNNNNNNGG 1 cut(s) 318
AflII CTTAAG 1 cut(s) 344
AgsI TTSAA 1 cut(s) 38
AluBI AGCT 3 cut(s) 115, 212, 290
AluI AGCT 3 cut(s) 115, 212, 290
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 2 cut(s) 54, 348
ApeKI GCWGC 2 cut(s) 290, 293
ApoI RAATTY 3 cut(s) 89, 197, 374
Asp700I GAANNNNTTC 1 cut(s) 134
AspLEI GCGC 2 cut(s) 164, 256
BbvI GCAGC 2 cut(s) 277, 280
BceAI ACGGC 1 cut(s) 175
BfaI CTAG 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 165
BfrI CTTAAG 1 cut(s) 344
BisI GCNGC 3 cut(s) 68, 291, 294
BlsI GCNGC 3 cut(s) 69, 292, 295
BpuEI CTTGAG 1 cut(s) 247
Bsa29I ATCGAT 1 cut(s) 192
BsaJI CCNNGG 2 cut(s) 51, 312
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse118I RCCGGY 1 cut(s) 70
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 2 cut(s) 51, 312
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 1 cut(s) 318
BseXI GCAGC 2 cut(s) 277, 280
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 2 cut(s) 56, 350
BshVI ATCGAT 1 cut(s) 192
BsiSI CCGG 2 cut(s) 71, 94
BslI CCNNNNNNNGG 1 cut(s) 318
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 2 cut(s) 56, 350
Bsp19I CCATGG 1 cut(s) 51
Bsp68I TCGCGA 1 cut(s) 372
BspACI CCGC 3 cut(s) 68, 267, 341
BspANI GGCC 2 cut(s) 56, 350
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 1 cut(s) 372
BspTI CTTAAG 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 70
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 2 cut(s) 51, 312
BssT1I CCWWGG 2 cut(s) 51, 312
Bst4CI ACNGT 2 cut(s) 135, 307
BstAFI CTTAAG 1 cut(s) 344
BstAPI GCANNNNNTGC 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 72
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 49
BstFNI CGCG 1 cut(s) 372
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 256
BstMWI GCNNNNNNNGC 4 cut(s) 218, 290, 299, 308
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 277, 280
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 2 cut(s) 56, 350
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 1 cut(s) 49
BtuMI TCGCGA 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 72
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 2 cut(s) 164, 256
Cfr10I RCCGGY 1 cut(s) 70
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 1 cut(s) 68
CviAII CATG 1 cut(s) 52
CviJI RGCY 8 cut(s) 13, 56, 67, 115, 212, 290, 311, 350
CviKI_1 RGCY 8 cut(s) 13, 56, 67, 115, 212, 290, 311, 350
EaeI YGGCCR 1 cut(s) 54
Eco130I CCWWGG 2 cut(s) 51, 312
Eco147I AGGCCT 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 89
EcoT14I CCWWGG 2 cut(s) 51, 312
ErhI CCWWGG 2 cut(s) 51, 312
FaeI CATG 1 cut(s) 55
FaiI YATR 2 cut(s) 53, 215
FatI CATG 1 cut(s) 51
Fnu4HI GCNGC 3 cut(s) 68, 291, 294
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 3 cut(s) 68, 291, 294
FspBI CTAG 1 cut(s) 299
GlaI GCGC 2 cut(s) 163, 255
GluI GCNGC 3 cut(s) 68, 291, 294
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 2 cut(s) 56, 350
HapII CCGG 2 cut(s) 71, 94
HgaI GACGC 1 cut(s) 68
HhaI GCGC 2 cut(s) 164, 256
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 2 cut(s) 162, 254
HinP1I GCGC 2 cut(s) 162, 254
HinfI GANTC 5 cut(s) 47, 76, 189, 233, 358
HpaII CCGG 2 cut(s) 71, 94
Hpy188I TCNGA 4 cut(s) 7, 188, 204, 363
Hpy188III TCNNGA 2 cut(s) 226, 371
Hpy99I CGWCG 4 cut(s) 29, 62, 161, 262
HpyAV CCTTC 1 cut(s) 312
HpyCH4III ACNGT 2 cut(s) 135, 307
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 290, 299, 308
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 2 cut(s) 162, 254
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
LpnPI CCDG 2 cut(s) 84, 107
Lsp1109I GCAGC 2 cut(s) 277, 280
MaeI CTAG 1 cut(s) 299
MboII GAAGA 1 cut(s) 134
MluCI AATT 4 cut(s) 33, 89, 197, 374
MlyI GAGTC 1 cut(s) 41
MnlI CCTC 3 cut(s) 79, 136, 181
MreI CGCCGGCG 1 cut(s) 70
MroNI GCCGGC 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 1 cut(s) 345
MspCI CTTAAG 1 cut(s) 344
MspI CCGG 2 cut(s) 71, 94
Mva1269I GAATGC 1 cut(s) 221
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 4 cut(s) 218, 290, 299, 308
NaeI GCCGGC 1 cut(s) 72
NcoI CCATGG 1 cut(s) 51
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 1 cut(s) 55
NruI TCGCGA 1 cut(s) 372
PceI AGGCCT 1 cut(s) 350
PctI GAATGC 1 cut(s) 221
PdiI GCCGGC 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 4 cut(s) 76, 189, 233, 358
PkrI GCNGC 3 cut(s) 69, 292, 295
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 290
RruI TCGCGA 1 cut(s) 372
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 3 cut(s) 68, 291, 294
SchI GAGTC 1 cut(s) 41
SetI ASST 3 cut(s) 117, 214, 292
SgrAI CRCCGGYG 1 cut(s) 70
SmlI CTYRAG 2 cut(s) 226, 344
SmoI CTYRAG 2 cut(s) 226, 344
Sse9I AATT 4 cut(s) 33, 89, 197, 374
SseBI AGGCCT 1 cut(s) 350
SsiI CCGC 3 cut(s) 68, 267, 341
SspMI CTAG 1 cut(s) 299
StuI AGGCCT 1 cut(s) 350
StyI CCWWGG 2 cut(s) 51, 312
TaaI ACNGT 2 cut(s) 135, 307
TaqI TCGA 2 cut(s) 192, 356
TasI AATT 4 cut(s) 33, 89, 197, 374
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 4 cut(s) 76, 189, 233, 358
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TseI GCWGC 2 cut(s) 290, 293
TspDTI ATGAA 2 cut(s) 45, 225
TspGWI ACGGA 1 cut(s) 56
Vha464I CTTAAG 1 cut(s) 344
XapI RAATTY 3 cut(s) 89, 197, 374
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.