Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0009972

Physical Location & Seq

Genomic Coordinates Reverse (-)

45850385 .. 45858790

Gene Structure 8406 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG184600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2112 bp

ATGGCCGAGGAGCAGCTAGCGGAGCTTCTGGAAACCCTAGGCGACTTCACAAGCAAAGAGAACTGGGACAAGTTCTTCACCATCCGAGGCACCGAAGACGCCTTCGAGTGGTACGCCGAGTGGTCCGAGCTCCGAGACCCTTTACTCTCCCACCTCCCGCCAAAACCCCAAATCCTCGTCCCCGGCTGCGGCAGCTCCCGCCTCTCCGAGCACCTCTACGACGCCGGCTTCACCTCCATCACCAACATCGACTTCTCCAAGGTCGCCATTTCCGATTGCCTCCGCCGCAATGTGCGCCTCAGGCCCCTTATGCGGTGGCGCGTCATGGACATGACCGCCATGCAGCTTGAGGCTGAGGCATTTGATGCTGTGGTTGACAAAGGTGGATTGGATGCGTTAATGGAGCCGGAGCTTGGCCCTAAGTTAGGGGATCAATATTTATCAGAGGTTAGAAGAGTTTTGAAGTCTGGAGGGAAATTTATTTGCCTTACATTGGCGGAGGCGCATGTTCTGGCTCTGCTTTTCCCGAAGTTCCGGTTTGGGTGGAAAATTAGTGTCCATGCAATACCTCACAAACCGTCTAGTAAGCCAAGTCTTCAAGCGTTTATGGTGGTTGCTGTAAAAGAGATGTCCTCTGAGTTGCAGGAGATCACATCCTCATTCAATAAGTCTTCTCTTGCTTGTAGAGGAAGTCAGGGTCGTGGACTTCTTGAAGCTGTTGAAAATGAGAATCAAATTCGTAGAGAATACTCCTCTGCTTCAGATATACAGCACTCTCTTGAAGACTTAAAACTTGGAGCTAGAGGAGATTTAACTAAGCTTCGCCCGGGTCGTCGTTTTCAGCTTACTTTGTGTGGGGGCTCCCACTTCAGTTGCAGAGCTGTAGTTCTTGATGCTAAGGAAACCTCTGGCCCCTTTGTATATCACTGTGGGGTTTTTATTGTGCCTAAGACTCGGGCTCATGAATGGCTGTTCTCTTCTGAAGAAGGACAATGGATGGTAGTAGAAAGCTCAAAGGCAGCTCGTCTAGTAATGGTTTTATTAGATGCCAGCCATGTTAGTGCGAGCATGGATGATATTCAGAAGGAATTATCTCCTTTGGTTAAACAATTGGCGCCTGGAAAAGATGACAATGGAGCTCAAATTCCATTCATGATGGCAAGTGATGGGATCAAACAACGAGATGTTGTGCACCAGGTCACATCCTCAATAACTGGACCAATAATAGTTGAGGACGTGATTTATGAAACTGATAATGGTGATATCAGTCGCATTCTACCATCCAGAGATTTGACATTTCGTCGCCTTGTCTTCCAAAGAAGTGAGGGTCTGGTTCAGTCTGAAGCTCTTCTGTCAGAGGAAGGATCGAAATATAAAATTGTTGGTCAGAGTGAGAAGAAAAAAACAAATTCATCTTCCAAATCTAAAAAAAGAGGAATCCAGAGACGAAGTGATGAGACGAGCCACCAGCTGAAGGTGTATCATGGGTATTTGGCTAGTTCTTATCATACAGGGATTATTTCTGGATTAATGTTGATTTCATCTTATTTGGAAAGCATGGTGTCAACACAAAAATCAGTCAAAACAGTTGTTATTGGTCTTGGAGCAGGTTTACTTCCTATGTTCCTTCATAAATGTATGCCCTTTATGCACATTGAGGCGGTGGAGTTGGATCCCATAGTCATCAAACTTGCAAAAGAATACTTTGGTTTCATCGAAGATGATCACTTGCAGGTGCACATTGCTGATGGGATTCAGTATCTCAGAAAAGCTGTACATTTTGCTGCGGAGGATGAAAAGTCTGCTGTTCGAGGAAATGAGGATGGTCACTGCAATTCTGACCCCCCATCTTCTAATGGAAGCCATTTGGCATTTCATGTAGAAGGCCAGGGAATTTCGAAGGTAGACATAGTCATTATTGATGTGGATTCTGCCGACTCAAGTTCTGGAATGACCTGCCCTGCTGCTGATTTTGTGGACAAGTCGTTTCTTCAGACTGTGAAGGATGCCCTTACTGAGAAGGGTCTATTTATTATAAATTTGGTTTCACGGTCCCAAGACATTAAAGACACTGTTATTTCAAGGATGAAAATGGTAAGAAAAGGAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

703

Amino Acids

78.06

Weight (kDa)

6.22

Isoelectric Point (pI)

44.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_25	PF13649	58 - 158	1.7e-10	Methyltransferase domain
Methyltransf_31	PF13847	59 - 166	3.8e-07	Methyltransferase domain
Methyltransf_11	PF08241	61 - 162	2.7e-12	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31740 AT2G31740
fragaria_vesca	FvH4_4g13510
malus_domestica	MD01G1010300.v1.1 MD04G1013700.v1.1
prunus_persica	Prupe.1G159900_v2.0.a1 Prupe.1G159900_v2.0.a1 Prupe.1G159900_v2.0.a1
pyrus_communis	pycom04g01070
rosa_chinensis	RchiOBHm_Chr4g0414141
rosa_laevigata	RLG00000008167
rosa_multiflora	Rmu_sc0000808.1_g000057 Rmu_sc0008865.1_g000003
rosa_roxburghii	Rroxscaffold_5G00358100
rosa_rugosa	Rorug04G0126400 Rorug04G0126500
rosa_samantha	Rh4AG184600 Rh4BG184000 Rh4CG196300 Rh4DG179900
rosa_wichuraiana	Rw4G015720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2036
AarI	CACCTGC	1 cut(s)	1723
Acc36I	ACCTGC	3 cut(s)	1598, 1723, 1964
AccB1I	GGYRCC	2 cut(s)	89, 1114
AccI	GTMKAC	1 cut(s)	1905
AccII	CGCG	1 cut(s)	321
AclWI	GGATC	5 cut(s)	438, 1178, 1372, 1667, 1680
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	6 cut(s)	476, 735, 1143, 1408, 1893, 2038
AcuI	CTGAAG	6 cut(s)	744, 853, 1002, 1362, 1493, 1976
AcyI	GRCGYC	3 cut(s)	99, 222, 1115
AfaI	GTAC	2 cut(s)	113, 1776
AfiI	CCNNNNNNNGG	7 cut(s)	108, 188, 312, 413, 425, 493, 1474
AgsI	TTSAA	7 cut(s)	463, 599, 664, 713, 722, 782, 2082
AhdI	GACNNNNNGTC	1 cut(s)	1299
AjiI	CACGTC	1 cut(s)	1237
AjnI	CCWGG	3 cut(s)	1117, 1194, 1887
AjuI	GAANNNNNNNTTGG	2 cut(s)	236, 268
Alw21I	GWGCWC	5 cut(s)	132, 213, 1141, 1194, 1740
Alw26I	GTCTC	3 cut(s)	129, 1438, 1451
Alw44I	GTGCAC	2 cut(s)	1190, 1736
AlwI	GGATC	5 cut(s)	438, 1178, 1372, 1667, 1680
Ama87I	CYCGRG	2 cut(s)	826, 954
AoxI	GGCC	5 cut(s)	3, 302, 415, 910, 1885
ApaLI	GTGCAC	2 cut(s)	1190, 1736
ApeKI	GCWGC	7 cut(s)	13, 186, 192, 343, 1019, 1784, 1964
ApoI	RAATTY	6 cut(s)	476, 735, 1143, 1408, 1893, 2038
AseI	ATTAAT	1 cut(s)	1529
Asp700I	GAANNNNTTC	1 cut(s)	1347
AspA2I	CCTAGG	1 cut(s)	37
AspLEI	GCGC	4 cut(s)	297, 321, 505, 1117
AspS9I	GGNCC	6 cut(s)	123, 303, 416, 911, 1217, 2052
AsuC2I	CCSGG	3 cut(s)	183, 827, 828
AsuHPI	GGTGA	4 cut(s)	70, 223, 232, 1271
AsuII	TTCGAA	1 cut(s)	1898
AsuNHI	GCTAGC	1 cut(s)	16
AvaI	CYCGRG	2 cut(s)	826, 954
AvaII	GGWCC	3 cut(s)	123, 1217, 2052
AvrII	CCTAGG	1 cut(s)	37
AxyI	CCTNAGG	1 cut(s)	299
BaeGI	GKGCMC	2 cut(s)	1194, 1740
BamHI	GGATCC	1 cut(s)	1672
BanI	GGYRCC	2 cut(s)	89, 1114
BanII	GRGCYC	4 cut(s)	132, 863, 961, 1141
BbsI	GAAGAC	5 cut(s)	102, 587, 663, 789, 1303
Bbv12I	GWGCWC	5 cut(s)	132, 213, 1141, 1194, 1740
BbvCI	CCTCAGC	1 cut(s)	354
BbvI	GCAGC	7 cut(s)	25, 173, 204, 355, 1031, 1771, 1951
BccI	CCATC	9 cut(s)	89, 245, 991, 1150, 1160, 1288, 1742, 1817, 1855
BciT130I	CCWGG	3 cut(s)	1119, 1196, 1889
BclI	TGATCA	1 cut(s)	1723
BcnI	CCSGG	3 cut(s)	183, 827, 828
BcoDI	GTCTC	3 cut(s)	129, 1438, 1451
BfaI	CTAG	6 cut(s)	17, 38, 582, 801, 1028, 1497
BfmI	CTRYAG	1 cut(s)	882
BfoI	RGCGCY	1 cut(s)	1118
BfuAI	ACCTGC	3 cut(s)	1598, 1723, 1964
BisI	GCNGC	9 cut(s)	14, 187, 190, 193, 286, 344, 1020, 1785, 1965
BlnI	CCTAGG	1 cut(s)	37
BlsI	GCNGC	9 cut(s)	15, 188, 191, 194, 287, 345, 1021, 1786, 1966
Bme1390I	CCNGG	6 cut(s)	183, 827, 828, 1119, 1196, 1889
Bme18I	GGWCC	3 cut(s)	123, 1217, 2052
BmeRI	GACNNNNNGTC	1 cut(s)	1299
BmeT110I	CYCGRG	2 cut(s)	826, 954
BmgBI	CACGTC	1 cut(s)	1237
BmgT120I	GGNCC	6 cut(s)	123, 303, 416, 911, 1217, 2052
BmiI	GGNNCC	8 cut(s)	91, 305, 405, 862, 913, 1116, 1674, 2054
BmrFI	CCNGG	6 cut(s)	183, 827, 828, 1119, 1196, 1889
BmrI	ACTGGG	1 cut(s)	73
BmsI	GCATC	5 cut(s)	355, 382, 883, 1036, 1996
BmtI	GCTAGC	1 cut(s)	20
BmuI	ACTGGG	1 cut(s)	73
BpiI	GAAGAC	5 cut(s)	102, 587, 663, 789, 1303
BplI	GAGNNNNNCTC	2 cut(s)	617, 649
BpmI	CTGGAG	1 cut(s)	489
Bpu10I	CCTNAGC	2 cut(s)	354, 897
Bpu14I	TTCGAA	1 cut(s)	1898
BpuEI	CTTGAG	2 cut(s)	368, 1924
BpuMI	CCSGG	3 cut(s)	183, 827, 828
BsaHI	GRCGYC	3 cut(s)	99, 222, 1115
BsaI	GGTCTC	1 cut(s)	129
BsaJI	CCNNGG	7 cut(s)	6, 37, 85, 181, 258, 826, 1888
BsaWI	WCCGGW	1 cut(s)	534
Bsc4I	CCNNNNNNNGG	7 cut(s)	108, 188, 312, 413, 425, 493, 1474
Bse118I	RCCGGY	1 cut(s)	224
Bse1I	ACTGG	2 cut(s)	68, 1219
Bse21I	CCTNAGG	1 cut(s)	299
Bse3DI	GCAATG	2 cut(s)	295, 1740
BseBI	CCWGG	3 cut(s)	1119, 1196, 1889
BseDI	CCNNGG	7 cut(s)	6, 37, 85, 181, 258, 826, 1888
BseLI	CCNNNNNNNGG	7 cut(s)	108, 188, 312, 413, 425, 493, 1474
BseMI	GCAATG	2 cut(s)	295, 1740
BseMII	CTCAG	5 cut(s)	313, 345, 627, 1777, 2007
BseNI	ACTGG	2 cut(s)	68, 1219
BseRI	GAGGAG	3 cut(s)	23, 742, 819
BseSI	GKGCMC	2 cut(s)	1194, 1740
BseXI	GCAGC	7 cut(s)	25, 173, 204, 355, 1031, 1771, 1951
Bsh1236I	CGCG	1 cut(s)	321
BshFI	GGCC	5 cut(s)	5, 304, 417, 912, 1887
BshNI	GGYRCC	2 cut(s)	89, 1114
BsiHKAI	GWGCWC	5 cut(s)	132, 213, 1141, 1194, 1740
BsiHKCI	CYCGRG	2 cut(s)	826, 954
BsiSI	CCGG	5 cut(s)	183, 225, 407, 535, 827
BslFI	GGGAC	3 cut(s)	80, 164, 2038
BslI	CCNNNNNNNGG	7 cut(s)	108, 188, 312, 413, 425, 493, 1474
BsmAI	GTCTC	3 cut(s)	129, 1438, 1451
BsmBI	CGTCTC	2 cut(s)	1438, 1451
BsmFI	GGGAC	3 cut(s)	80, 164, 2038
BsmI	GAATGC	1 cut(s)	1272
BsnI	GGCC	5 cut(s)	5, 304, 417, 912, 1887
Bso31I	GGTCTC	1 cut(s)	129
BsoBI	CYCGRG	2 cut(s)	826, 954
Bsp119I	TTCGAA	1 cut(s)	1898
Bsp1286I	GDGCHC	7 cut(s)	132, 213, 863, 961, 1141, 1194, 1740
Bsp1407I	TGTACA	1 cut(s)	1774
Bsp143I	GATC	6 cut(s)	430, 648, 1170, 1364, 1672, 1723
BspANI	GGCC	5 cut(s)	5, 304, 417, 912, 1887
BspCNI	CTCAG	5 cut(s)	312, 346, 628, 1776, 2008
BspFNI	CGCG	1 cut(s)	321
BspHI	TCATGA	2 cut(s)	961, 1152
BspLI	GGNNCC	8 cut(s)	91, 305, 405, 862, 913, 1116, 1674, 2054
BspMI	ACCTGC	3 cut(s)	1598, 1723, 1964
BspOI	GCTAGC	1 cut(s)	20
BspPI	GGATC	5 cut(s)	438, 1178, 1372, 1667, 1680
BspQI	GCTCTTC	1 cut(s)	1353
BspT104I	TTCGAA	1 cut(s)	1898
BspT107I	GGYRCC	2 cut(s)	89, 1114
BspTNI	GGTCTC	1 cut(s)	129
BsrDI	GCAATG	2 cut(s)	295, 1740
BsrFI	RCCGGY	1 cut(s)	224
BsrGI	TGTACA	1 cut(s)	1774
BsrI	ACTGG	2 cut(s)	68, 1219
BssAI	RCCGGY	1 cut(s)	224
BssECI	CCNNGG	7 cut(s)	6, 37, 85, 181, 258, 826, 1888
BssMI	GATC	6 cut(s)	430, 648, 1170, 1364, 1672, 1723
BssNI	GRCGYC	3 cut(s)	99, 222, 1115
BssT1I	CCWWGG	2 cut(s)	37, 258
Bst2UI	CCWGG	3 cut(s)	1119, 1196, 1889
Bst4CI	ACNGT	6 cut(s)	579, 929, 1588, 1999, 2052, 2074
Bst6I	CTCTTC	3 cut(s)	448, 982, 1353
BstACI	GRCGYC	3 cut(s)	99, 222, 1115
BstAPI	GCANNNNNTGC	1 cut(s)	365
BstAUI	TGTACA	1 cut(s)	1774
BstBI	TTCGAA	1 cut(s)	1898
BstC8I	GCNNGC	4 cut(s)	18, 226, 1051, 1066
BstDEI	CTNAG	9 cut(s)	299, 354, 420, 636, 816, 897, 948, 1763, 2016
BstENI	CCTNNNNNAGG	1 cut(s)	423
BstFNI	CGCG	1 cut(s)	321
BstH2I	RGCGCY	1 cut(s)	1118
BstHHI	GCGC	4 cut(s)	297, 321, 505, 1117
BstKTI	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
BstMAI	GTCTC	3 cut(s)	129, 1438, 1451
BstMBI	GATC	6 cut(s)	430, 648, 1170, 1364, 1672, 1723
BstMWI	GCNNNNNNNGC	9 cut(s)	22, 192, 198, 285, 294, 301, 310, 365, 1648
BstNI	CCWGG	3 cut(s)	1119, 1196, 1889
BstNSI	RCATGY	1 cut(s)	509
BstSCI	CCNGG	6 cut(s)	181, 825, 826, 1117, 1194, 1887
BstSFI	CTRYAG	1 cut(s)	882
BstSLI	GKGCMC	2 cut(s)	1194, 1740
BstUI	CGCG	1 cut(s)	321
BstV1I	GCAGC	7 cut(s)	25, 173, 204, 355, 1031, 1771, 1951
BstV2I	GAAGAC	5 cut(s)	102, 587, 663, 789, 1303
BstX2I	RGATCY	1 cut(s)	1672
BstYI	RGATCY	1 cut(s)	1672
Bsu36I	CCTNAGG	1 cut(s)	299
BsuRI	GGCC	5 cut(s)	5, 304, 417, 912, 1887
BtrI	CACGTC	1 cut(s)	1237
BtsI	GCAGTG	1 cut(s)	1828
BtsIMutI	CAGTG	3 cut(s)	925, 1828, 2070
BveI	ACCTGC	3 cut(s)	1598, 1723, 1964
Cac8I	GCNNGC	4 cut(s)	18, 226, 1051, 1066
CciI	TCATGA	2 cut(s)	961, 1152
CfoI	GCGC	4 cut(s)	297, 321, 505, 1117
Cfr10I	RCCGGY	1 cut(s)	224
Cfr13I	GGNCC	6 cut(s)	123, 303, 416, 911, 1217, 2052
Cfr9I	CCCGGG	1 cut(s)	826
CseI	GACGC	3 cut(s)	107, 230, 310
CsiI	ACCWGGT	1 cut(s)	1194
Csp6I	GTAC	2 cut(s)	112, 1775
CspCI	CAANNNNNGTGG	2 cut(s)	854, 889
CviQI	GTAC	2 cut(s)	112, 1775
DdeI	CTNAG	9 cut(s)	299, 354, 420, 636, 816, 897, 948, 1763, 2016
DinI	GGCGCC	1 cut(s)	1116
DpnI	GATC	6 cut(s)	432, 650, 1172, 1366, 1674, 1725
DpnII	GATC	6 cut(s)	430, 648, 1170, 1364, 1672, 1723
DriI	GACNNNNNGTC	1 cut(s)	1299
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	448, 982, 1353
Eam1105I	GACNNNNNGTC	1 cut(s)	1299
EarI	CTCTTC	3 cut(s)	448, 982, 1353
EciI	GGCGGA	2 cut(s)	272, 512
Ecl136II	GAGCTC	2 cut(s)	130, 1139
Eco130I	CCWWGG	2 cut(s)	37, 258
Eco24I	GRGCYC	4 cut(s)	132, 863, 961, 1141
Eco31I	GGTCTC	1 cut(s)	129
Eco32I	GATATC	1 cut(s)	1264
Eco47I	GGWCC	3 cut(s)	123, 1217, 2052
Eco53kI	GAGCTC	2 cut(s)	130, 1139
Eco57I	CTGAAG	6 cut(s)	744, 853, 1002, 1362, 1493, 1976
Eco81I	CCTNAGG	1 cut(s)	299
Eco88I	CYCGRG	2 cut(s)	826, 954
EcoICRI	GAGCTC	2 cut(s)	130, 1139
EcoNI	CCTNNNNNAGG	1 cut(s)	423
EcoO109I	RGGNCCY	1 cut(s)	303
EcoRII	CCWGG	3 cut(s)	1117, 1194, 1887
EcoRV	GATATC	1 cut(s)	1264
EcoT14I	CCWWGG	2 cut(s)	37, 258
EcoT38I	GRGCYC	4 cut(s)	132, 863, 961, 1141
EgeI	GGCGCC	1 cut(s)	1116
EheI	GGCGCC	1 cut(s)	1116
ErhI	CCWWGG	2 cut(s)	37, 258
Esp3I	CGTCTC	2 cut(s)	1438, 1451
FaqI	GGGAC	3 cut(s)	80, 164, 2038
FauI	CCCGC	2 cut(s)	165, 206
FbaI	TGATCA	1 cut(s)	1723
FblI	GTMKAC	1 cut(s)	1905
Fnu4HI	GCNGC	9 cut(s)	14, 187, 190, 193, 286, 344, 1020, 1785, 1965
FriOI	GRGCYC	4 cut(s)	132, 863, 961, 1141
Fsp4HI	GCNGC	9 cut(s)	14, 187, 190, 193, 286, 344, 1020, 1785, 1965
FspBI	CTAG	6 cut(s)	17, 38, 582, 801, 1028, 1497
GlaI	GCGC	4 cut(s)	296, 320, 504, 1116
GluI	GCNGC	9 cut(s)	14, 187, 190, 193, 286, 344, 1020, 1785, 1965
GsuI	CTGGAG	1 cut(s)	489
HaeII	RGCGCY	1 cut(s)	1118
HaeIII	GGCC	5 cut(s)	5, 304, 417, 912, 1887
HapII	CCGG	5 cut(s)	183, 225, 407, 535, 827
HgaI	GACGC	3 cut(s)	107, 230, 310
HhaI	GCGC	4 cut(s)	297, 321, 505, 1117
Hin1I	GRCGYC	3 cut(s)	99, 222, 1115
Hin6I	GCGC	4 cut(s)	295, 319, 503, 1115
HinP1I	GCGC	4 cut(s)	295, 319, 503, 1115
HincII	GTYRAC	2 cut(s)	376, 1566
HindII	GTYRAC	2 cut(s)	376, 1566
HindIII	AAGCTT	1 cut(s)	818
HinfI	GANTC	6 cut(s)	730, 952, 1437, 1753, 1928, 1937
HpaII	CCGG	5 cut(s)	183, 225, 407, 535, 827
HphI	GGTGA	4 cut(s)	70, 223, 232, 1271
Hpy166II	GTNNAC	8 cut(s)	376, 704, 1192, 1566, 1613, 1738, 1906, 1978
Hpy8I	GTNNAC	8 cut(s)	376, 704, 1192, 1566, 1613, 1738, 1906, 1978
Hpy99I	CGWCG	3 cut(s)	224, 837, 1305
HpyCH4III	ACNGT	6 cut(s)	579, 929, 1588, 1999, 2052, 2074
HpyCH4IV	ACGT	1 cut(s)	1236
HpyF10VI	GCNNNNNNNGC	9 cut(s)	22, 192, 198, 285, 294, 301, 310, 365, 1648
HpyF3I	CTNAG	9 cut(s)	299, 354, 420, 636, 816, 897, 948, 1763, 2016
HpySE526I	ACGT	1 cut(s)	1236
Hsp92I	GRCGYC	3 cut(s)	99, 222, 1115
HspAI	GCGC	4 cut(s)	295, 319, 503, 1115
KasI	GGCGCC	1 cut(s)	1114
KroI	GCCGGC	1 cut(s)	224
KroNI	GCCGGC	1 cut(s)	226
Ksp22I	TGATCA	1 cut(s)	1723
Kzo9I	GATC	6 cut(s)	430, 648, 1170, 1364, 1672, 1723
LguI	GCTCTTC	1 cut(s)	1353
Lsp1109I	GCAGC	7 cut(s)	25, 173, 204, 355, 1031, 1771, 1951
LweI	GCATC	5 cut(s)	355, 382, 883, 1036, 1996
MabI	ACCWGGT	1 cut(s)	1194
MaeI	CTAG	6 cut(s)	17, 38, 582, 801, 1028, 1497
MaeII	ACGT	1 cut(s)	1236
MaeIII	GTNAC	2 cut(s)	1198, 1826
MalI	GATC	6 cut(s)	432, 650, 1172, 1366, 1674, 1725
MboI	GATC	6 cut(s)	430, 648, 1170, 1364, 1672, 1723
MfeI	CAATTG	1 cut(s)	1109
MflI	RGATCY	1 cut(s)	1672
MhlI	GDGCHC	7 cut(s)	132, 213, 863, 961, 1141, 1194, 1740
Mly113I	GGCGCC	1 cut(s)	1115
MlyI	GAGTC	2 cut(s)	946, 1931
MmeI	TCCRAC	1 cut(s)	1650
MroNI	GCCGGC	1 cut(s)	224
MroXI	GAANNNNTTC	1 cut(s)	1347
MseI	TTAA	6 cut(s)	398, 788, 812, 1104, 1529, 2064
MslI	CAYNNNNRTG	2 cut(s)	329, 1059
MspA1I	CMGCKG	1 cut(s)	1471
MspI	CCGG	5 cut(s)	183, 225, 407, 535, 827
MspR9I	CCNGG	6 cut(s)	183, 827, 828, 1119, 1196, 1889
MunI	CAATTG	1 cut(s)	1109
Mva1269I	GAATGC	1 cut(s)	1272
MvaI	CCWGG	3 cut(s)	1119, 1196, 1889
MvnI	CGCG	1 cut(s)	321
MwoI	GCNNNNNNNGC	9 cut(s)	22, 192, 198, 285, 294, 301, 310, 365, 1648
NaeI	GCCGGC	1 cut(s)	226
NarI	GGCGCC	1 cut(s)	1115
NciI	CCSGG	3 cut(s)	183, 827, 828
NdeII	GATC	6 cut(s)	430, 648, 1170, 1364, 1672, 1723
NgoMIV	GCCGGC	1 cut(s)	224
NheI	GCTAGC	1 cut(s)	16
NlaIV	GGNNCC	8 cut(s)	91, 305, 405, 862, 913, 1116, 1674, 2054
NmeAIII	GCCGAG	2 cut(s)	31, 142
NmuCI	GTSAC	2 cut(s)	1198, 1826
NspI	RCATGY	1 cut(s)	509
NspV	TTCGAA	1 cut(s)	1898
PagI	TCATGA	2 cut(s)	961, 1152
PaqCI	CACCTGC	1 cut(s)	1723
PciSI	GCTCTTC	1 cut(s)	1353
PcsI	WCGNNNNNNNCGW	2 cut(s)	270, 829
PctI	GAATGC	1 cut(s)	1272
PdiI	GCCGGC	1 cut(s)	226
PdmI	GAANNNNTTC	1 cut(s)	1347
PfeI	GAWTC	4 cut(s)	730, 1437, 1753, 1928
PflFI	GACNNNGTC	1 cut(s)	1910
PkrI	GCNGC	9 cut(s)	15, 188, 191, 194, 287, 345, 1021, 1786, 1966
PleI	GAGTC	2 cut(s)	946, 1931
PluTI	GGCGCC	1 cut(s)	1118
PpsI	GAGTC	2 cut(s)	946, 1931
PshBI	ATTAAT	1 cut(s)	1529
PsiI	TTATAA	1 cut(s)	2036
Psp124BI	GAGCTC	2 cut(s)	132, 1141
Psp6I	CCWGG	3 cut(s)	1117, 1194, 1887
PspGI	CCWGG	3 cut(s)	1117, 1194, 1887
PspN4I	GGNNCC	8 cut(s)	91, 305, 405, 862, 913, 1116, 1674, 2054
PspPI	GGNCC	6 cut(s)	123, 303, 416, 911, 1217, 2052
PsuI	RGATCY	1 cut(s)	1672
PsyI	GACNNNGTC	1 cut(s)	1910
PvuII	CAGCTG	1 cut(s)	1471
RsaI	GTAC	2 cut(s)	113, 1776
RsaNI	GTAC	2 cut(s)	112, 1775
RseI	CAYNNNNRTG	2 cut(s)	329, 1059
SacI	GAGCTC	2 cut(s)	132, 1141
SapI	GCTCTTC	1 cut(s)	1353
SaqAI	TTAA	6 cut(s)	398, 788, 812, 1104, 1529, 2064
SatI	GCNGC	9 cut(s)	14, 187, 190, 193, 286, 344, 1020, 1785, 1965
Sau3AI	GATC	6 cut(s)	430, 648, 1170, 1364, 1672, 1723
Sau96I	GGNCC	6 cut(s)	123, 303, 416, 911, 1217, 2052
SchI	GAGTC	2 cut(s)	946, 1931
ScrFI	CCNGG	6 cut(s)	183, 827, 828, 1119, 1196, 1889
SduI	GDGCHC	7 cut(s)	132, 213, 863, 961, 1141, 1194, 1740
SexAI	ACCWGGT	1 cut(s)	1194
SfaNI	GCATC	5 cut(s)	355, 382, 883, 1036, 1996
SfcI	CTRYAG	1 cut(s)	882
SfoI	GGCGCC	1 cut(s)	1116
SfuI	TTCGAA	1 cut(s)	1898
SinI	GGWCC	3 cut(s)	123, 1217, 2052
SmaI	CCCGGG	1 cut(s)	828
SmiMI	CAYNNNNRTG	2 cut(s)	329, 1059
SmlI	CTYRAG	2 cut(s)	347, 1939
SmoI	CTYRAG	2 cut(s)	347, 1939
SspDI	GGCGCC	1 cut(s)	1114
SspI	AATATT	1 cut(s)	437
SspMI	CTAG	6 cut(s)	17, 38, 582, 801, 1028, 1497
SstI	GAGCTC	2 cut(s)	132, 1141
StyD4I	CCNGG	6 cut(s)	181, 825, 826, 1117, 1194, 1887
StyI	CCWWGG	2 cut(s)	37, 258
TaaI	ACNGT	6 cut(s)	579, 929, 1588, 1999, 2052, 2074
TaiI	ACGT	1 cut(s)	1239
TaqI	TCGA	6 cut(s)	105, 249, 1367, 1716, 1810, 1898
TatI	WGTACW	1 cut(s)	1774
TauI	GCSGC	2 cut(s)	192, 288
TfiI	GAWTC	4 cut(s)	730, 1437, 1753, 1928
Tru1I	TTAA	6 cut(s)	398, 788, 812, 1104, 1529, 2064
Tru9I	TTAA	6 cut(s)	398, 788, 812, 1104, 1529, 2064
TscAI	CASTG	3 cut(s)	932, 1835, 2077
TseFI	GTSAC	2 cut(s)	1198, 1826
TseI	GCWGC	7 cut(s)	13, 186, 192, 343, 1019, 1784, 1964
Tsp45I	GTSAC	2 cut(s)	1198, 1826
TspMI	CCCGGG	1 cut(s)	826
TspRI	CASTG	3 cut(s)	932, 1835, 2077
Tth111I	GACNNNGTC	1 cut(s)	1910
VneI	GTGCAC	2 cut(s)	1190, 1736
VpaK11BI	GGWCC	3 cut(s)	123, 1217, 2052
VspI	ATTAAT	1 cut(s)	1529
XagI	CCTNNNNNAGG	1 cut(s)	423
XapI	RAATTY	6 cut(s)	476, 735, 1143, 1408, 1893, 2038
XceI	RCATGY	1 cut(s)	509
XmaI	CCCGGG	1 cut(s)	826
XmaJI	CCTAGG	1 cut(s)	37
XmiI	GTMKAC	1 cut(s)	1905
XmnI	GAANNNNTTC	1 cut(s)	1347
XspI	CTAG	6 cut(s)	17, 38, 582, 801, 1028, 1497

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.