MD04G1013700.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009972
Physical Location & Seq
Forward (+)
1563042 .. 1571418
8377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1013700.v1.1.491

Sequence Viewer

Length: 2274 bp
ATGGGGAAGAAGGAGGAGCAGTTAGCAGAGCTACTCGGAACCCTAGGAGACTTCACCAGCAAAGAGAACTGGGACAAGTTCTTCACCATCCGCGGCACCGACGACTCCTTCGAGTGGTACGCCGAGTGGTCCGAGCTCAGAGATCCTTTGCTCTCCCACCTCCCTCCCCAACCCCAAATTCTCATCCCCGGCTGCGGCAGCTCCCGCCTCTCCGAACACCTCTACGACGCCGGCTTCACTTCCATCACCAACATTGACTTCTCCAAGGTCGCCATTTCCGACTGCCTCCGCCGCAATGTTCGCCACCGCCCCGACATGCGCTGGCGCGTCATGGACATGACCGCCATGCAGTTTTCGGAGGAGGCATTTGATGTTGTAGTTGACAAAGGTGGACTGGATGCCTTAATGGAGCCAGAGATGGGGCCTAAGCTTGGGGATCAGTATTTATCAGAGGTGAGAAGAGTTTTGAAGTGTGGGGGGAAATTTGTTTGCCTTACACTGGCAGAGTCACATGTTCTAGCTTTGCTTTTCTCGAAGTACCGGTTTGGGTGGAAAATGGGTGTTCATGCCATACCTCAGAAACCGTCTAGTAAGCCAAGCCTTCAAGCATATATGGTGGTTGCTGAGAAAGAGGTTTCCAGCGTGTTGCAGGATGTCACAACATCTTTCAATAAGTCTTCTTTTGCTTGTAGGGGAAGTCAGGCTTCTGGACTTCTTGAAGCTGTTGATAAGGAGAACCAAATTCGTAGAGAATACTCCCTTGGTTCGGATGTACTGTACTCTCTTGAAGAGTTAAATCTTGGAGCTAGAGGGGATTTGACTAAGCTTTCCCCAGGCAGTCGTTTTCAGCTTAATTTGGGCGGTGGATCCCGTTTCAGTTACAGAGCTGTAGTTCTTGATGCTCAGGATTCATCTGGTCCATTTGCGTATCATTGTGGGGTTTTTATTGTGCCTAAGACTCGGGCTCATGAATGGCTATTCTCCTCTGAAGAAGGACAATGGATGGTAGTAGAAAGCTCTAAGGCAGCTCGTCTAGTAATGATTTTATTAGATGCCAGCCATGTTAGTGCCAGCATGGATGACATTCAGAAGGATTTATCTCCCTTGGTTAAACAATTGGCGCCTGGAAAAGATGACAATGGAGCCCAGATTCCATTCATGATGGCAAGTGATGGGATCAAGCAACGAGATATTGTTCACCAGGTCACATCAACAATAACTGGCCCAATTATAGTTGAGGACGTGATTTATGAAACTGTCGATGGTGATATCAGTCGCATTCTACCATCCAGAGATTTGACGTTTCGTCGCCTTGTTTTCCAAAGAAGTGAGGGTTTGGTTCAGTCTGAAGCTCTTCTATCAGAGGAAGGATCTAACAGTAAAGTTGTTGGTGAGACAGAGAGAAAAAAGACAAATTCATCTTCCAGATCTAAAAGAAAAGGAATCCAGAAAAGAAGTGGTGAAACGAGCCACCAGCTGAAGGTCTATCATGGATATTTGGCTAGTTCTTACCATACAGGGATTATTTCTGGATTAATGTTGATCTCTTCTTATTTGGAAAGCATGGCATCAGCTCAAAAATCAGTTAAAGCAGTTGTAATTGGTCTTGGAGCAGGTTTACTTCCTATGTTCCTTAACAGATGTCTGCCCTTTGTGCACACTGAGGTGGTGGAGTTGGATCCTGTAGTCCTCAAACTTGCAAAAGAATACTTTGGTTTTGTTGAAGATGATTGCTTACAGGCGCACGTTGCTGATGGGATTCAGTTTGTTAGAAATATTGCAAATTCTGCTGCTTCTGATGAAACTTCTCTTGTTCAAGACAAGAAGGATGCTCAATGCAACACTTCTTCCAATGGTGATTTCGAAAGCAGGGTGATTTCGAAGGTTGACATACTTATTATTGATGTGGATTCGGCAGACTCAAGCTCTGGGATGACCTGTCCTGCAGCAGATTTCGTGGACGAATCATTTCTTCAGACTGTAAAGGATGCACTTTCTGAGAAAGGTCTATTTATTATTAATTTGGTATCGCGGTCACAAGCCATTAAAGACACTGTTATTTCGAGGATGAAGGTGGTGTTTAGCCATCTCTTCTCCCTTCAGCTTGAAGAAGACGTCAATGAAGTTATCTTTGGTCTTTGCTCGGCTTCTTGTATCGAGGAGGTTTGCTTCCCTGAAGCCGCTCTTCAACTTGAGAAATTACTAAAACTGGAACACCCGGAGATAAGCCAAAGCATTATAAATACTACGAAGAAGTTGAGACATTTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

758

Amino Acids

83.8

Weight (kDa)

5.53

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_25 PF13649 61 - 160 1.5e-11 Methyltransferase domain
Methyltransf_11 PF08241 63 - 164 4.8e-12 Methyltransferase domain
Methyltransf_31 PF13847 63 - 168 8.4e-09 Methyltransferase domain
Methyltransf_12 PF08242 64 - 162 4.8e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2240
AatII GACGTC 1 cut(s) 2118
Acc36I ACCTGC 1 cut(s) 1604
AccB1I GGYRCC 2 cut(s) 95, 1120
AccBSI CCGCTC 1 cut(s) 2183
AccII CGCG 3 cut(s) 93, 327, 2032
AclWI GGATC 8 cut(s) 137, 444, 861, 874, 1184, 1378, 1673, 1686
AcsI RAATTY 5 cut(s) 177, 482, 741, 1414, 1783
AcuI CTGAAG 6 cut(s) 1008, 1368, 1499, 1958, 2084, 2196
AcyI GRCGYC 3 cut(s) 228, 1121, 2115
AfaI GTAC 4 cut(s) 119, 539, 774, 779
AfiI CCNNNNNNNGG 7 cut(s) 114, 194, 419, 431, 499, 766, 1480
AflIII ACRYGT 1 cut(s) 511
AgeI ACCGGT 1 cut(s) 540
AhdI GACNNNNNGTC 1 cut(s) 1305
AjiI CACGTC 1 cut(s) 1243
AjnI CCWGG 3 cut(s) 832, 1123, 1200
AleI CACNNNNGTG 1 cut(s) 1664
Alw21I GWGCWC 2 cut(s) 138, 1659
Alw26I GTCTC 3 cut(s) 42, 1388, 2254
Alw44I GTGCAC 1 cut(s) 1655
AlwI GGATC 8 cut(s) 137, 444, 861, 874, 1184, 1378, 1673, 1686
Ama87I CYCGRG 1 cut(s) 960
AoxI GGCC 2 cut(s) 422, 1222
ApaLI GTGCAC 1 cut(s) 1655
ApeKI GCWGC 5 cut(s) 192, 198, 1025, 1790, 1946
ApoI RAATTY 5 cut(s) 177, 482, 741, 1414, 1783
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
AseI ATTAAT 2 cut(s) 1535, 2019
AsiGI ACCGGT 1 cut(s) 540
Asp700I GAANNNNTTC 2 cut(s) 1353, 1968
AspA2I CCTAGG 1 cut(s) 43
AspLEI GCGC 4 cut(s) 321, 327, 1123, 1744
AspS9I GGNCC 4 cut(s) 129, 422, 917, 1223
AsuC2I CCSGG 2 cut(s) 189, 2219
AsuII TTCGAA 2 cut(s) 1863, 1880
AvaI CYCGRG 1 cut(s) 960
AvaII GGWCC 2 cut(s) 129, 917
AvrII CCTAGG 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 1659
BamHI GGATCC 2 cut(s) 866, 1678
BanI GGYRCC 2 cut(s) 95, 1120
BanII GRGCYC 3 cut(s) 138, 967, 1147
BbsI GAAGAC 2 cut(s) 669, 2118
Bbv12I GWGCWC 2 cut(s) 138, 1659
BbvI GCAGC 5 cut(s) 179, 210, 1037, 1777, 1958
BciT130I CCWGG 3 cut(s) 834, 1125, 1202
BcnI CCSGG 2 cut(s) 189, 2219
BcoDI GTCTC 3 cut(s) 42, 1388, 2254
BfaI CTAG 6 cut(s) 44, 518, 588, 807, 1034, 1503
BfmI CTRYAG 3 cut(s) 888, 1683, 1944
BfoI RGCGCY 1 cut(s) 1124
BfuAI ACCTGC 1 cut(s) 1604
BglII AGATCT 1 cut(s) 1427
BisI GCNGC 9 cut(s) 94, 193, 196, 199, 292, 1026, 1791, 1947, 2181
BlnI CCTAGG 1 cut(s) 43
BlsI GCNGC 9 cut(s) 95, 194, 197, 200, 293, 1027, 1792, 1948, 2182
Bme1390I CCNGG 5 cut(s) 189, 834, 1125, 1202, 2219
Bme18I GGWCC 2 cut(s) 129, 917
BmeRI GACNNNNNGTC 1 cut(s) 1305
BmeT110I CYCGRG 1 cut(s) 960
BmgBI CACGTC 1 cut(s) 1243
BmgT120I GGNCC 4 cut(s) 129, 422, 917, 1223
BmiI GGNNCC 8 cut(s) 40, 97, 411, 423, 868, 1122, 1144, 1680
BmrFI CCNGG 5 cut(s) 189, 834, 1125, 1202, 2219
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 6 cut(s) 388, 889, 1042, 1577, 1819, 1978
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 2 cut(s) 669, 2118
Bpu10I CCTNAGC 2 cut(s) 426, 903
Bpu14I TTCGAA 2 cut(s) 1863, 1880
BpuEI CTTGAG 2 cut(s) 1906, 2213
BpuMI CCSGG 2 cut(s) 189, 2219
BsaHI GRCGYC 3 cut(s) 228, 1121, 2115
BsaJI CCNNGG 7 cut(s) 43, 91, 187, 264, 760, 832, 1104
BsaWI WCCGGW 1 cut(s) 540
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 7 cut(s) 114, 194, 419, 431, 499, 766, 1480
Bse118I RCCGGY 2 cut(s) 230, 540
Bse1I ACTGG 5 cut(s) 74, 399, 504, 1225, 2214
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 3 cut(s) 834, 1125, 1202
BseDI CCNNGG 7 cut(s) 43, 91, 187, 264, 760, 832, 1104
BseLI CCNNNNNNNGG 7 cut(s) 114, 194, 419, 431, 499, 766, 1480
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 6 cut(s) 151, 590, 615, 917, 1653, 1989
BseNI ACTGG 5 cut(s) 74, 399, 504, 1225, 2214
BseRI GAGGAG 4 cut(s) 29, 374, 973, 2174
BseSI GKGCMC 1 cut(s) 1659
BseXI GCAGC 5 cut(s) 179, 210, 1037, 1777, 1958
Bsh1236I CGCG 3 cut(s) 93, 327, 2032
BshFI GGCC 2 cut(s) 424, 1224
BshNI GGYRCC 2 cut(s) 95, 1120
BshTI ACCGGT 1 cut(s) 540
BsiHKAI GWGCWC 2 cut(s) 138, 1659
BsiHKCI CYCGRG 1 cut(s) 960
BsiSI CCGG 4 cut(s) 189, 231, 541, 2219
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 7 cut(s) 114, 194, 419, 431, 499, 766, 1480
BsmAI GTCTC 3 cut(s) 42, 1388, 2254
BsmFI GGGAC 1 cut(s) 86
BsmI GAATGC 1 cut(s) 1278
BsnI GGCC 2 cut(s) 424, 1224
BsoBI CYCGRG 1 cut(s) 960
Bsp119I TTCGAA 2 cut(s) 1863, 1880
Bsp1286I GDGCHC 4 cut(s) 138, 967, 1147, 1659
Bsp143I GATC 8 cut(s) 142, 436, 866, 1176, 1370, 1427, 1542, 1678
BspANI GGCC 2 cut(s) 424, 1224
BspCNI CTCAG 6 cut(s) 150, 589, 616, 916, 1654, 1990
BspFNI CGCG 3 cut(s) 93, 327, 2032
BspHI TCATGA 2 cut(s) 967, 1158
BspLI GGNNCC 8 cut(s) 40, 97, 411, 423, 868, 1122, 1144, 1680
BspMAI CTGCAG 1 cut(s) 1948
BspMI ACCTGC 1 cut(s) 1604
BspPI GGATC 8 cut(s) 137, 444, 861, 874, 1184, 1378, 1673, 1686
BspQI GCTCTTC 2 cut(s) 1359, 2190
BspT104I TTCGAA 2 cut(s) 1863, 1880
BspT107I GGYRCC 2 cut(s) 95, 1120
BsrBI CCGCTC 1 cut(s) 2183
BsrDI GCAATG 1 cut(s) 301
BsrFI RCCGGY 2 cut(s) 230, 540
BsrI ACTGG 5 cut(s) 74, 399, 504, 1225, 2214
BssAI RCCGGY 2 cut(s) 230, 540
BssECI CCNNGG 7 cut(s) 43, 91, 187, 264, 760, 832, 1104
BssMI GATC 8 cut(s) 142, 436, 866, 1176, 1370, 1427, 1542, 1678
BssNI GRCGYC 3 cut(s) 228, 1121, 2115
BssT1I CCWWGG 4 cut(s) 43, 264, 760, 1104
Bst2UI CCWGG 3 cut(s) 834, 1125, 1202
Bst4CI ACNGT 6 cut(s) 585, 777, 1258, 1379, 1981, 2056
Bst6I CTCTTC 6 cut(s) 454, 783, 1359, 1552, 2096, 2190
BstACI GRCGYC 3 cut(s) 228, 1121, 2115
BstAPI GCANNNNNTGC 1 cut(s) 1787
BstBI TTCGAA 2 cut(s) 1863, 1880
BstC8I GCNNGC 4 cut(s) 232, 323, 1057, 1072
BstDSI CCRYGG 1 cut(s) 91
BstFNI CGCG 3 cut(s) 93, 327, 2032
BstH2I RGCGCY 1 cut(s) 1124
BstHHI GCGC 4 cut(s) 321, 327, 1123, 1744
BstKTI GATC 8 cut(s) 145, 439, 869, 1179, 1373, 1430, 1545, 1681
BstMAI GTCTC 3 cut(s) 42, 1388, 2254
BstMBI GATC 8 cut(s) 142, 436, 866, 1176, 1370, 1427, 1542, 1678
BstMWI GCNNNNNNNGC 7 cut(s) 198, 204, 291, 300, 1654, 1748, 1787
BstNI CCWGG 3 cut(s) 834, 1125, 1202
BstNSI RCATGY 2 cut(s) 319, 515
BstSCI CCNGG 5 cut(s) 187, 832, 1123, 1200, 2217
BstSFI CTRYAG 3 cut(s) 888, 1683, 1944
BstSLI GKGCMC 1 cut(s) 1659
BstUI CGCG 3 cut(s) 93, 327, 2032
BstV1I GCAGC 5 cut(s) 179, 210, 1037, 1777, 1958
BstV2I GAAGAC 2 cut(s) 669, 2118
BstX2I RGATCY 5 cut(s) 142, 866, 1370, 1427, 1678
BstYI RGATCY 5 cut(s) 142, 866, 1370, 1427, 1678
BsuRI GGCC 2 cut(s) 424, 1224
BtgI CCRYGG 1 cut(s) 91
BtrI CACGTC 1 cut(s) 1243
BtsIMutI CAGTG 3 cut(s) 497, 1659, 2052
BveI ACCTGC 1 cut(s) 1604
Cac8I GCNNGC 4 cut(s) 232, 323, 1057, 1072
CciI TCATGA 2 cut(s) 967, 1158
CfoI GCGC 4 cut(s) 321, 327, 1123, 1744
Cfr10I RCCGGY 2 cut(s) 230, 540
Cfr13I GGNCC 4 cut(s) 129, 422, 917, 1223
Cfr42I CCGCGG 1 cut(s) 94
CseI GACGC 2 cut(s) 236, 316
CsiI ACCWGGT 1 cut(s) 1200
Csp6I GTAC 4 cut(s) 118, 538, 773, 778
CspAI ACCGGT 1 cut(s) 540
CviQI GTAC 4 cut(s) 118, 538, 773, 778
DinI GGCGCC 1 cut(s) 1122
DpnI GATC 8 cut(s) 144, 438, 868, 1178, 1372, 1429, 1544, 1680
DpnII GATC 8 cut(s) 142, 436, 866, 1176, 1370, 1427, 1542, 1678
DriI GACNNNNNGTC 1 cut(s) 1305
Eam1104I CTCTTC 6 cut(s) 454, 783, 1359, 1552, 2096, 2190
Eam1105I GACNNNNNGTC 1 cut(s) 1305
EarI CTCTTC 6 cut(s) 454, 783, 1359, 1552, 2096, 2190
EciI GGCGGA 1 cut(s) 278
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 4 cut(s) 43, 264, 760, 1104
Eco24I GRGCYC 3 cut(s) 138, 967, 1147
Eco32I GATATC 1 cut(s) 1270
Eco47I GGWCC 2 cut(s) 129, 917
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 6 cut(s) 1008, 1368, 1499, 1958, 2084, 2196
Eco88I CYCGRG 1 cut(s) 960
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 422
EcoRII CCWGG 3 cut(s) 832, 1123, 1200
EcoRV GATATC 1 cut(s) 1270
EcoT14I CCWWGG 4 cut(s) 43, 264, 760, 1104
EcoT38I GRGCYC 3 cut(s) 138, 967, 1147
EgeI GGCGCC 1 cut(s) 1122
EheI GGCGCC 1 cut(s) 1122
ErhI CCWWGG 4 cut(s) 43, 264, 760, 1104
FalI AAGNNNNNCTT 4 cut(s) 688, 720, 2169, 2201
FaqI GGGAC 1 cut(s) 86
FauI CCCGC 1 cut(s) 212
Fnu4HI GCNGC 9 cut(s) 94, 193, 196, 199, 292, 1026, 1791, 1947, 2181
FriOI GRGCYC 3 cut(s) 138, 967, 1147
Fsp4HI GCNGC 9 cut(s) 94, 193, 196, 199, 292, 1026, 1791, 1947, 2181
FspBI CTAG 6 cut(s) 44, 518, 588, 807, 1034, 1503
GlaI GCGC 4 cut(s) 320, 326, 1122, 1743
GluI GCNGC 9 cut(s) 94, 193, 196, 199, 292, 1026, 1791, 1947, 2181
HaeII RGCGCY 1 cut(s) 1124
HaeIII GGCC 2 cut(s) 424, 1224
HapII CCGG 4 cut(s) 189, 231, 541, 2219
HgaI GACGC 2 cut(s) 236, 316
HhaI GCGC 4 cut(s) 321, 327, 1123, 1744
Hin1I GRCGYC 3 cut(s) 228, 1121, 2115
Hin6I GCGC 4 cut(s) 319, 325, 1121, 1742
HinP1I GCGC 4 cut(s) 319, 325, 1121, 1742
HincII GTYRAC 2 cut(s) 382, 1888
HindII GTYRAC 2 cut(s) 382, 1888
HindIII AAGCTT 2 cut(s) 428, 824
HpaII CCGG 4 cut(s) 189, 231, 541, 2219
Hpy166II GTNNAC 7 cut(s) 382, 392, 1198, 1619, 1657, 1888, 1960
Hpy8I GTNNAC 7 cut(s) 382, 392, 1198, 1619, 1657, 1888, 1960
Hpy99I CGWCG 3 cut(s) 104, 230, 1311
HpyCH4III ACNGT 6 cut(s) 585, 777, 1258, 1379, 1981, 2056
HpyCH4IV ACGT 4 cut(s) 1242, 1301, 1746, 2115
HpyCH4V TGCA 8 cut(s) 349, 649, 1657, 1700, 1781, 1839, 1946, 1991
HpyF10VI GCNNNNNNNGC 7 cut(s) 198, 204, 291, 300, 1654, 1748, 1787
HpySE526I ACGT 4 cut(s) 1242, 1301, 1746, 2115
Hsp92I GRCGYC 3 cut(s) 228, 1121, 2115
HspAI GCGC 4 cut(s) 319, 325, 1121, 1742
KasI GGCGCC 1 cut(s) 1120
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
KspI CCGCGG 1 cut(s) 94
Kzo9I GATC 8 cut(s) 142, 436, 866, 1176, 1370, 1427, 1542, 1678
LguI GCTCTTC 2 cut(s) 1359, 2190
LmnI GCTCC 6 cut(s) 16, 206, 409, 803, 1142, 1610
Lsp1109I GCAGC 5 cut(s) 179, 210, 1037, 1777, 1958
LweI GCATC 6 cut(s) 388, 889, 1042, 1577, 1819, 1978
MabI ACCWGGT 1 cut(s) 1200
MaeI CTAG 6 cut(s) 44, 518, 588, 807, 1034, 1503
MaeII ACGT 4 cut(s) 1242, 1301, 1746, 2115
MaeIII GTNAC 5 cut(s) 507, 655, 878, 1204, 2034
MalI GATC 8 cut(s) 144, 438, 868, 1178, 1372, 1429, 1544, 1680
MbiI CCGCTC 1 cut(s) 2183
MboI GATC 8 cut(s) 142, 436, 866, 1176, 1370, 1427, 1542, 1678
MfeI CAATTG 1 cut(s) 1115
MflI RGATCY 5 cut(s) 142, 866, 1370, 1427, 1678
MhlI GDGCHC 4 cut(s) 138, 967, 1147, 1659
Mly113I GGCGCC 1 cut(s) 1121
MlyI GAGTC 4 cut(s) 98, 515, 952, 1913
MmeI TCCRAC 2 cut(s) 303, 1656
MroNI GCCGGC 1 cut(s) 230
MroXI GAANNNNTTC 2 cut(s) 1353, 1968
MseI TTAA 9 cut(s) 404, 794, 852, 1110, 1535, 1587, 1635, 2019, 2046
MslI CAYNNNNRTG 3 cut(s) 335, 1065, 1664
MspA1I CMGCKG 2 cut(s) 93, 1477
MspI CCGG 4 cut(s) 189, 231, 541, 2219
MspR9I CCNGG 5 cut(s) 189, 834, 1125, 1202, 2219
MunI CAATTG 1 cut(s) 1115
Mva1269I GAATGC 1 cut(s) 1278
MvaI CCWGG 3 cut(s) 834, 1125, 1202
MvnI CGCG 3 cut(s) 93, 327, 2032
MwoI GCNNNNNNNGC 7 cut(s) 198, 204, 291, 300, 1654, 1748, 1787
NaeI GCCGGC 1 cut(s) 232
NarI GGCGCC 1 cut(s) 1121
NciI CCSGG 2 cut(s) 189, 2219
NdeII GATC 8 cut(s) 142, 436, 866, 1176, 1370, 1427, 1542, 1678
NgoMIV GCCGGC 1 cut(s) 230
NlaIV GGNNCC 8 cut(s) 40, 97, 411, 423, 868, 1122, 1144, 1680
NmeAIII GCCGAG 2 cut(s) 148, 2123
NmuCI GTSAC 4 cut(s) 507, 655, 1204, 2034
NspI RCATGY 2 cut(s) 319, 515
NspV TTCGAA 2 cut(s) 1863, 1880
OliI CACNNNNGTG 1 cut(s) 1664
PagI TCATGA 2 cut(s) 967, 1158
PciI ACATGT 1 cut(s) 511
PciSI GCTCTTC 2 cut(s) 1359, 2190
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 276
PctI GAATGC 1 cut(s) 1278
PdiI GCCGGC 1 cut(s) 232
PdmI GAANNNNTTC 2 cut(s) 1353, 1968
PfeI GAWTC 6 cut(s) 908, 1150, 1443, 1759, 1910, 1964
PinAI ACCGGT 1 cut(s) 540
PkrI GCNGC 9 cut(s) 95, 194, 197, 200, 293, 1027, 1792, 1948, 2182
PleI GAGTC 4 cut(s) 98, 514, 952, 1913
PluTI GGCGCC 1 cut(s) 1124
PpsI GAGTC 4 cut(s) 98, 514, 952, 1913
PscI ACATGT 1 cut(s) 511
PshBI ATTAAT 2 cut(s) 1535, 2019
PsiI TTATAA 1 cut(s) 2240
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 832, 1123, 1200
PspGI CCWGG 3 cut(s) 832, 1123, 1200
PspN4I GGNNCC 8 cut(s) 40, 97, 411, 423, 868, 1122, 1144, 1680
PspPI GGNCC 4 cut(s) 129, 422, 917, 1223
PstI CTGCAG 1 cut(s) 1948
PsuI RGATCY 5 cut(s) 142, 866, 1370, 1427, 1678
PvuII CAGCTG 1 cut(s) 1477
RsaI GTAC 4 cut(s) 119, 539, 774, 779
RsaNI GTAC 4 cut(s) 118, 538, 773, 778
RseI CAYNNNNRTG 3 cut(s) 335, 1065, 1664
SacI GAGCTC 1 cut(s) 138
SacII CCGCGG 1 cut(s) 94
SapI GCTCTTC 2 cut(s) 1359, 2190
SaqAI TTAA 9 cut(s) 404, 794, 852, 1110, 1535, 1587, 1635, 2019, 2046
SatI GCNGC 9 cut(s) 94, 193, 196, 199, 292, 1026, 1791, 1947, 2181
Sau3AI GATC 8 cut(s) 142, 436, 866, 1176, 1370, 1427, 1542, 1678
Sau96I GGNCC 4 cut(s) 129, 422, 917, 1223
SchI GAGTC 4 cut(s) 98, 515, 952, 1913
ScrFI CCNGG 5 cut(s) 189, 834, 1125, 1202, 2219
SduI GDGCHC 4 cut(s) 138, 967, 1147, 1659
SexAI ACCWGGT 1 cut(s) 1200
SfaNI GCATC 6 cut(s) 388, 889, 1042, 1577, 1819, 1978
SfcI CTRYAG 3 cut(s) 888, 1683, 1944
SfoI GGCGCC 1 cut(s) 1122
Sfr303I CCGCGG 1 cut(s) 94
SfuI TTCGAA 2 cut(s) 1863, 1880
SgrBI CCGCGG 1 cut(s) 94
SinI GGWCC 2 cut(s) 129, 917
SmiMI CAYNNNNRTG 3 cut(s) 335, 1065, 1664
SmlI CTYRAG 2 cut(s) 1921, 2192
SmoI CTYRAG 2 cut(s) 1921, 2192
SspDI GGCGCC 1 cut(s) 1120
SspI AATATT 1 cut(s) 1777
SspMI CTAG 6 cut(s) 44, 518, 588, 807, 1034, 1503
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 5 cut(s) 187, 832, 1123, 1200, 2217
StyI CCWWGG 4 cut(s) 43, 264, 760, 1104
TaaI ACNGT 6 cut(s) 585, 777, 1258, 1379, 1981, 2056
TaiI ACGT 4 cut(s) 1245, 1304, 1749, 2118
TaqI TCGA 7 cut(s) 111, 533, 1260, 1863, 1880, 2063, 2157
TatI WGTACW 2 cut(s) 772, 777
TauI GCSGC 4 cut(s) 96, 198, 294, 2183
TfiI GAWTC 6 cut(s) 908, 1150, 1443, 1759, 1910, 1964
Tru1I TTAA 9 cut(s) 404, 794, 852, 1110, 1535, 1587, 1635, 2019, 2046
Tru9I TTAA 9 cut(s) 404, 794, 852, 1110, 1535, 1587, 1635, 2019, 2046
TscAI CASTG 3 cut(s) 504, 1666, 2059
TseFI GTSAC 4 cut(s) 507, 655, 1204, 2034
TseI GCWGC 5 cut(s) 192, 198, 1025, 1790, 1946
Tsp45I GTSAC 4 cut(s) 507, 655, 1204, 2034
TspDTI ATGAA 9 cut(s) 554, 900, 984, 1147, 1266, 1407, 1815, 2084, 2136
TspRI CASTG 3 cut(s) 504, 1666, 2059
VneI GTGCAC 1 cut(s) 1655
VpaK11BI GGWCC 2 cut(s) 129, 917
VspI ATTAAT 2 cut(s) 1535, 2019
XapI RAATTY 5 cut(s) 177, 482, 741, 1414, 1783
XceI RCATGY 2 cut(s) 319, 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 1454
XmaJI CCTAGG 1 cut(s) 43
XmnI GAANNNNTTC 2 cut(s) 1353, 1968
XspI CTAG 6 cut(s) 44, 518, 588, 807, 1034, 1503
ZraI GACGTC 1 cut(s) 2116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.