Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0009972

Physical Location & Seq

Genomic Coordinates Reverse (-)

32233618 .. 32241156

Gene Structure 7539 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG184000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGGCCAAGGAGGAGCAGCTAGCGGAGCTTCTGGAAACCCTAGGCGACTTCACAAGCAAAGAGAACTGGGACAAGTTCTTCACCATCCGAGGCACCGAAGACGCCTTCGAGTGGTACGCCGAGTGGTCCGAGCTCCGAGACCCTTTGCTCTCCCACCTCCCGCCAAAACCCCAAATCCTCGTCCCCGGCTGCGGCAGCTCCCGCCTCTCCGAGCACCTCTACGACGCCGGCTTCACCTCCATCACCAACATCGACTTCTCCAAGGTCGCCATTTCCGATTGCCTCCGCCGCAATGTGCGCCTGAGGCCCCTTATGCGGTGGCGCGTCATGGACATGACCGCCATGCAGCTTGAGGATGAGGCATTTGATGCTGTGGTTGACAAGGGTGGATTGGATGCGTTAATGGAGCCGGAGCTCGGCTCTAAGTTAGGGGATCAATATTTATCAGAGGTTAGAAGAGTTTTGAAGTCTGGAGGGAAATTCATTTGCCTTACATTGGCGGAGTCGCATGTTCTGGCTCTGCTTTTCCCGAAGTTCCGGTTTGGGTGGAAAATTAGTGTCCATGCAATACCTCACAAACCGTCTAGTAAGCCAAGTCTTCAAGCGTTTATGGTGGTTGCTGTAAAAGAGATGTCCACTGAGTTGCAGGAGATCACATCCTCATTCAATAAGTCTTCCCTTGCTTGTAGAGGAAGTCAGGGTCGTGGACTTCTTGAAGCTGTTGAAAATGAGAATCAAATTCGTAGAGAATACTCCTCTGCTTCAGATATACGGCACTCTCTTGAAGACTTAAAACTTGGAGCTAGAGGAGATTTAACTAAGCTTCGCCCGGGTCGTCGTTTTCAGCTTACTTTGTGTGGGGGCTCCCACTTCAGTTGCAGAGCTGTAGTTCTTGATGCTAAGGAAAACTCTGGCCCCTTTGTATATCACTGTGGGGTTTTTATTGTTCCTAAGACTCGGGCTCATGAATGGCTGTTCTCTTCTGAAGAAGGACAATGGATGGTAGTAGAAAGCTCAAAGGCAGCTCGTCTAGTAATGGTTTTATTAGATGCCAGCCATGTTAGTGCGAGCATGGATGATATTCAGAAGGAATTATCTCCTTTGGTTAAACAATTGGCGCCTGGAAAAGATGACAATGGAGCTCAAATTCCATTCATGATGGCAAGTGATGGGATCAAACAACGAGATATTGTGCACCAGGTCACATCCTCAATAACTGGACCAATAATAGTTGAGGATGTGATTTATGAAACTGATAATGGTGATATCAGTCGCATTCTACCATCCAGAGATTTGACATTTCGTCGCCTTGTCTTCCAAAGAAGTGAGGGTCTGGTTCAGTCTGAAGCTCTTCTGTCAGAGGAAGGATCGAAATATAAAATTGTTGGTCAGAGTGAGAAGAAAAAAACAAATTCATCTTCCAAATCTAAAAAAAGAGGAATCCAGAGACGAAGTGATGAGACGAGCCACCAGCTGAAGGTGTATCATGGGTATTTGGCTAGTTCTTATCATACAGGGATTATTTCTGGATTAATGTTGATTTCATCTTATTTGGAAAGCATGGTGTCAACACAAAAATCAGTCAAAACAGTTGTTATTGGTCTTGGAGCAGGTTTACTTCCTATGTTCCTTCATAAATGTATGCCCTTTATGCACATTGAGGCGGTGGAGTTGGATCCCATAGTCATCAAACTTGCAAAAGAATACTTTGGTTTCATCGAAGATGATCACTTGCAGGTGCACATTGCTGATGGGATTCAGTATCTCAGAAAAGCTGTACATTTTGCTGCGGAGGATGAAAAGTCTGCTGTTCGAGGAATTGAGGGTCACTGCAATTCTGACCCCCCATCTTCTAATGGAAGCCATTTGGCATTTCATGTAGAAGGCCAGGGAATTTCGAAGGTAGACATAGTCATTATTGATGTGGATTCTGCTGACTCAAGTTCTGGAATGACCTGCCCTGCAGCTGATTTTGTGGACGAGTCGTTTCTTCGGACTGTGAAGGATGCCCTTACTGAGAAGGGTCTATTTATTATAAATTTGGTTTCACGGTCCCAAGACATTAAAGACACTGTTATTTCAAGGATGAAAATGGTGTTTAGCCATCTCTTCTGCCTTCAGCTTGAAGAAGATGTCAATGAAGTTATTTTTGCCCTTCCTTCAGCTTCCTGTATCAAGGAGGATGGCTTCGCCAAAGCCACTCTTCAACTTGAGAAATTGTTAAAATTGGAGCACCCGGAGATAAGCCAAAGCATTATAGATGCTACAAAGAAGATAAGACATCTGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

763

Amino Acids

85.03

Weight (kDa)

6.12

Isoelectric Point (pI)

46.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_25	PF13649	59 - 159	4.8e-11	Methyltransferase domain
Methyltransf_31	PF13847	60 - 167	3e-08	Methyltransferase domain
Methyltransf_11	PF08241	62 - 163	3.7e-13	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31740 AT2G31740
fragaria_vesca	FvH4_4g13510
malus_domestica	MD01G1010300.v1.1 MD04G1013700.v1.1
prunus_persica	Prupe.1G159900_v2.0.a1 Prupe.1G159900_v2.0.a1 Prupe.1G159900_v2.0.a1
pyrus_communis	pycom04g01070
rosa_chinensis	RchiOBHm_Chr4g0414141
rosa_laevigata	RLG00000008167
rosa_multiflora	Rmu_sc0000808.1_g000057 Rmu_sc0008865.1_g000003
rosa_roxburghii	Rroxscaffold_5G00358100
rosa_rugosa	Rorug04G0126400 Rorug04G0126500
rosa_samantha	Rh4AG184600 Rh4BG184000 Rh4CG196300 Rh4DG179900
rosa_wichuraiana	Rw4G015720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2036
AarI	CACCTGC	1 cut(s)	1726
Acc36I	ACCTGC	3 cut(s)	1601, 1726, 1964
AccB1I	GGYRCC	2 cut(s)	92, 1117
AccI	GTMKAC	1 cut(s)	1905
AccII	CGCG	1 cut(s)	324
AclWI	GGATC	5 cut(s)	441, 1181, 1375, 1670, 1683
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	6 cut(s)	479, 738, 1146, 1411, 1893, 2038
AcuI	CTGAAG	7 cut(s)	747, 856, 1005, 1365, 1496, 2102, 2145
AcyI	GRCGYC	3 cut(s)	102, 225, 1118
AfaI	GTAC	2 cut(s)	116, 1779
AfiI	CCNNNNNNNGG	6 cut(s)	111, 191, 315, 416, 496, 1477
AgsI	TTSAA	9 cut(s)	466, 602, 667, 716, 725, 785, 2082, 2126, 2207
AhdI	GACNNNNNGTC	1 cut(s)	1302
AjnI	CCWGG	3 cut(s)	1120, 1197, 1887
AjuI	GAANNNNNNNTTGG	2 cut(s)	239, 271
Alw21I	GWGCWC	7 cut(s)	135, 216, 417, 1144, 1197, 1743, 2235
Alw26I	GTCTC	3 cut(s)	132, 1441, 1454
Alw44I	GTGCAC	2 cut(s)	1193, 1739
AlwI	GGATC	5 cut(s)	441, 1181, 1375, 1670, 1683
Ama87I	CYCGRG	2 cut(s)	829, 957
AoxI	GGCC	4 cut(s)	3, 305, 913, 1885
ApaLI	GTGCAC	2 cut(s)	1193, 1739
ApeKI	GCWGC	7 cut(s)	16, 189, 195, 346, 1022, 1787, 1964
ApoI	RAATTY	6 cut(s)	479, 738, 1146, 1411, 1893, 2038
AseI	ATTAAT	1 cut(s)	1532
Asp700I	GAANNNNTTC	1 cut(s)	1350
AspA2I	CCTAGG	1 cut(s)	40
AspLEI	GCGC	3 cut(s)	300, 324, 1120
AspS9I	GGNCC	5 cut(s)	126, 306, 914, 1220, 2052
AsuC2I	CCSGG	4 cut(s)	186, 830, 831, 2237
AsuHPI	GGTGA	4 cut(s)	73, 226, 235, 1274
AsuII	TTCGAA	1 cut(s)	1898
AsuNHI	GCTAGC	1 cut(s)	19
AvaI	CYCGRG	2 cut(s)	829, 957
AvaII	GGWCC	3 cut(s)	126, 1220, 2052
AvrII	CCTAGG	1 cut(s)	40
AxyI	CCTNAGG	1 cut(s)	302
BaeGI	GKGCMC	2 cut(s)	1197, 1743
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	1675
BanI	GGYRCC	2 cut(s)	92, 1117
BanII	GRGCYC	5 cut(s)	135, 417, 866, 964, 1144
BbsI	GAAGAC	5 cut(s)	105, 590, 666, 792, 1306
Bbv12I	GWGCWC	7 cut(s)	135, 216, 417, 1144, 1197, 1743, 2235
BbvI	GCAGC	7 cut(s)	28, 176, 207, 358, 1034, 1774, 1976
BceAI	ACGGC	1 cut(s)	788
BciT130I	CCWGG	3 cut(s)	1122, 1199, 1889
BclI	TGATCA	1 cut(s)	1726
BcnI	CCSGG	4 cut(s)	186, 830, 831, 2237
BcoDI	GTCTC	3 cut(s)	132, 1441, 1454
BfaI	CTAG	6 cut(s)	20, 41, 585, 804, 1031, 1500
BfmI	CTRYAG	2 cut(s)	885, 1962
BfoI	RGCGCY	1 cut(s)	1121
BfuAI	ACCTGC	3 cut(s)	1601, 1726, 1964
BisI	GCNGC	9 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1788, 1965
BlnI	CCTAGG	1 cut(s)	40
BlsI	GCNGC	9 cut(s)	18, 191, 194, 197, 290, 348, 1024, 1789, 1966
Bme1390I	CCNGG	7 cut(s)	186, 830, 831, 1122, 1199, 1889, 2237
Bme18I	GGWCC	3 cut(s)	126, 1220, 2052
BmeRI	GACNNNNNGTC	1 cut(s)	1302
BmeT110I	CYCGRG	2 cut(s)	829, 957
BmgT120I	GGNCC	5 cut(s)	126, 306, 914, 1220, 2052
BmiI	GGNNCC	8 cut(s)	94, 308, 408, 865, 916, 1119, 1677, 2054
BmrFI	CCNGG	7 cut(s)	186, 830, 831, 1122, 1199, 1889, 2237
BmrI	ACTGGG	1 cut(s)	76
BmsI	GCATC	6 cut(s)	358, 385, 886, 1039, 1996, 2251
BmtI	GCTAGC	1 cut(s)	23
BmuI	ACTGGG	1 cut(s)	76
BpiI	GAAGAC	5 cut(s)	105, 590, 666, 792, 1306
BplI	GAGNNNNNCTC	2 cut(s)	404, 436
BpmI	CTGGAG	1 cut(s)	492
Bpu10I	CCTNAGC	1 cut(s)	900
Bpu14I	TTCGAA	1 cut(s)	1898
BpuEI	CTTGAG	3 cut(s)	371, 1924, 2231
BpuMI	CCSGG	4 cut(s)	186, 830, 831, 2237
BsaHI	GRCGYC	3 cut(s)	102, 225, 1118
BsaI	GGTCTC	1 cut(s)	132
BsaJI	CCNNGG	7 cut(s)	6, 40, 88, 184, 261, 829, 1888
BsaWI	WCCGGW	1 cut(s)	537
Bsc4I	CCNNNNNNNGG	6 cut(s)	111, 191, 315, 416, 496, 1477
Bse118I	RCCGGY	1 cut(s)	227
Bse1I	ACTGG	2 cut(s)	71, 1222
Bse21I	CCTNAGG	1 cut(s)	302
Bse3DI	GCAATG	2 cut(s)	298, 1743
BseBI	CCWGG	3 cut(s)	1122, 1199, 1889
BseDI	CCNNGG	7 cut(s)	6, 40, 88, 184, 261, 829, 1888
BseLI	CCNNNNNNNGG	6 cut(s)	111, 191, 315, 416, 496, 1477
BseMI	GCAATG	2 cut(s)	298, 1743
BseMII	CTCAG	4 cut(s)	293, 630, 1780, 2007
BseNI	ACTGG	2 cut(s)	71, 1222
BseRI	GAGGAG	3 cut(s)	26, 745, 822
BseSI	GKGCMC	2 cut(s)	1197, 1743
BseXI	GCAGC	7 cut(s)	28, 176, 207, 358, 1034, 1774, 1976
Bsh1236I	CGCG	1 cut(s)	324
BshFI	GGCC	4 cut(s)	5, 307, 915, 1887
BshNI	GGYRCC	2 cut(s)	92, 1117
BsiHKAI	GWGCWC	7 cut(s)	135, 216, 417, 1144, 1197, 1743, 2235
BsiHKCI	CYCGRG	2 cut(s)	829, 957
BsiSI	CCGG	6 cut(s)	186, 228, 410, 538, 830, 2237
BslFI	GGGAC	3 cut(s)	83, 167, 2038
BslI	CCNNNNNNNGG	6 cut(s)	111, 191, 315, 416, 496, 1477
BsmAI	GTCTC	3 cut(s)	132, 1441, 1454
BsmBI	CGTCTC	2 cut(s)	1441, 1454
BsmFI	GGGAC	3 cut(s)	83, 167, 2038
BsmI	GAATGC	1 cut(s)	1275
BsnI	GGCC	4 cut(s)	5, 307, 915, 1887
Bso31I	GGTCTC	1 cut(s)	132
BsoBI	CYCGRG	2 cut(s)	829, 957
Bsp119I	TTCGAA	1 cut(s)	1898
Bsp1286I	GDGCHC	9 cut(s)	135, 216, 417, 866, 964, 1144, 1197, 1743, 2235
Bsp1407I	TGTACA	1 cut(s)	1777
Bsp143I	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
BspANI	GGCC	4 cut(s)	5, 307, 915, 1887
BspCNI	CTCAG	4 cut(s)	294, 631, 1779, 2008
BspFNI	CGCG	1 cut(s)	324
BspHI	TCATGA	2 cut(s)	964, 1155
BspLI	GGNNCC	8 cut(s)	94, 308, 408, 865, 916, 1119, 1677, 2054
BspMAI	CTGCAG	1 cut(s)	1966
BspMI	ACCTGC	3 cut(s)	1601, 1726, 1964
BspOI	GCTAGC	1 cut(s)	23
BspPI	GGATC	5 cut(s)	441, 1181, 1375, 1670, 1683
BspQI	GCTCTTC	1 cut(s)	1356
BspT104I	TTCGAA	1 cut(s)	1898
BspT107I	GGYRCC	2 cut(s)	92, 1117
BspTNI	GGTCTC	1 cut(s)	132
BsrDI	GCAATG	2 cut(s)	298, 1743
BsrFI	RCCGGY	1 cut(s)	227
BsrGI	TGTACA	1 cut(s)	1777
BsrI	ACTGG	2 cut(s)	71, 1222
BssAI	RCCGGY	1 cut(s)	227
BssECI	CCNNGG	7 cut(s)	6, 40, 88, 184, 261, 829, 1888
BssMI	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
BssNI	GRCGYC	3 cut(s)	102, 225, 1118
BssT1I	CCWWGG	3 cut(s)	6, 40, 261
Bst2UI	CCWGG	3 cut(s)	1122, 1199, 1889
Bst4CI	ACNGT	6 cut(s)	582, 932, 1591, 1999, 2052, 2074
Bst6I	CTCTTC	5 cut(s)	451, 985, 1356, 2114, 2208
BstACI	GRCGYC	3 cut(s)	102, 225, 1118
BstAPI	GCANNNNNTGC	1 cut(s)	368
BstAUI	TGTACA	1 cut(s)	1777
BstBI	TTCGAA	1 cut(s)	1898
BstC8I	GCNNGC	4 cut(s)	21, 229, 1054, 1069
BstDEI	CTNAG	8 cut(s)	302, 423, 639, 819, 900, 951, 1766, 2016
BstFNI	CGCG	1 cut(s)	324
BstH2I	RGCGCY	1 cut(s)	1121
BstHHI	GCGC	3 cut(s)	300, 324, 1120
BstKTI	GATC	6 cut(s)	436, 654, 1176, 1370, 1678, 1729
BstMAI	GTCTC	3 cut(s)	132, 1441, 1454
BstMBI	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
BstMWI	GCNNNNNNNGC	9 cut(s)	25, 195, 201, 288, 297, 304, 313, 368, 1651
BstNI	CCWGG	3 cut(s)	1122, 1199, 1889
BstNSI	RCATGY	1 cut(s)	512
BstSCI	CCNGG	7 cut(s)	184, 828, 829, 1120, 1197, 1887, 2235
BstSFI	CTRYAG	2 cut(s)	885, 1962
BstSLI	GKGCMC	2 cut(s)	1197, 1743
BstUI	CGCG	1 cut(s)	324
BstV1I	GCAGC	7 cut(s)	28, 176, 207, 358, 1034, 1774, 1976
BstV2I	GAAGAC	5 cut(s)	105, 590, 666, 792, 1306
BstX2I	RGATCY	1 cut(s)	1675
BstYI	RGATCY	1 cut(s)	1675
Bsu36I	CCTNAGG	1 cut(s)	302
BsuRI	GGCC	4 cut(s)	5, 307, 915, 1887
BtsI	GCAGTG	1 cut(s)	1828
BtsIMutI	CAGTG	4 cut(s)	636, 928, 1828, 2070
BveI	ACCTGC	3 cut(s)	1601, 1726, 1964
Cac8I	GCNNGC	4 cut(s)	21, 229, 1054, 1069
CciI	TCATGA	2 cut(s)	964, 1155
CfoI	GCGC	3 cut(s)	300, 324, 1120
Cfr10I	RCCGGY	1 cut(s)	227
Cfr13I	GGNCC	5 cut(s)	126, 306, 914, 1220, 2052
Cfr9I	CCCGGG	1 cut(s)	829
CseI	GACGC	3 cut(s)	110, 233, 313
CsiI	ACCWGGT	1 cut(s)	1197
Csp6I	GTAC	2 cut(s)	115, 1778
CspCI	CAANNNNNGTGG	2 cut(s)	857, 892
CviQI	GTAC	2 cut(s)	115, 1778
DdeI	CTNAG	8 cut(s)	302, 423, 639, 819, 900, 951, 1766, 2016
DinI	GGCGCC	1 cut(s)	1119
DpnI	GATC	6 cut(s)	435, 653, 1175, 1369, 1677, 1728
DpnII	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
DriI	GACNNNNNGTC	1 cut(s)	1302
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	5 cut(s)	451, 985, 1356, 2114, 2208
Eam1105I	GACNNNNNGTC	1 cut(s)	1302
EarI	CTCTTC	5 cut(s)	451, 985, 1356, 2114, 2208
EciI	GGCGGA	2 cut(s)	275, 515
Ecl136II	GAGCTC	3 cut(s)	133, 415, 1142
Eco130I	CCWWGG	3 cut(s)	6, 40, 261
Eco24I	GRGCYC	5 cut(s)	135, 417, 866, 964, 1144
Eco31I	GGTCTC	1 cut(s)	132
Eco32I	GATATC	1 cut(s)	1267
Eco47I	GGWCC	3 cut(s)	126, 1220, 2052
Eco53kI	GAGCTC	3 cut(s)	133, 415, 1142
Eco57I	CTGAAG	7 cut(s)	747, 856, 1005, 1365, 1496, 2102, 2145
Eco81I	CCTNAGG	1 cut(s)	302
Eco88I	CYCGRG	2 cut(s)	829, 957
EcoICRI	GAGCTC	3 cut(s)	133, 415, 1142
EcoO109I	RGGNCCY	1 cut(s)	306
EcoRII	CCWGG	3 cut(s)	1120, 1197, 1887
EcoRV	GATATC	1 cut(s)	1267
EcoT14I	CCWWGG	3 cut(s)	6, 40, 261
EcoT38I	GRGCYC	5 cut(s)	135, 417, 866, 964, 1144
EgeI	GGCGCC	1 cut(s)	1119
EheI	GGCGCC	1 cut(s)	1119
ErhI	CCWWGG	3 cut(s)	6, 40, 261
Esp3I	CGTCTC	2 cut(s)	1441, 1454
FalI	AAGNNNNNCTT	2 cut(s)	2187, 2219
FaqI	GGGAC	3 cut(s)	83, 167, 2038
FauI	CCCGC	2 cut(s)	168, 209
FbaI	TGATCA	1 cut(s)	1726
FblI	GTMKAC	1 cut(s)	1905
Fnu4HI	GCNGC	9 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1788, 1965
FriOI	GRGCYC	5 cut(s)	135, 417, 866, 964, 1144
Fsp4HI	GCNGC	9 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1788, 1965
FspBI	CTAG	6 cut(s)	20, 41, 585, 804, 1031, 1500
GlaI	GCGC	3 cut(s)	299, 323, 1119
GluI	GCNGC	9 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1788, 1965
GsuI	CTGGAG	1 cut(s)	492
HaeII	RGCGCY	1 cut(s)	1121
HaeIII	GGCC	4 cut(s)	5, 307, 915, 1887
HapII	CCGG	6 cut(s)	186, 228, 410, 538, 830, 2237
HgaI	GACGC	3 cut(s)	110, 233, 313
HhaI	GCGC	3 cut(s)	300, 324, 1120
Hin1I	GRCGYC	3 cut(s)	102, 225, 1118
Hin6I	GCGC	3 cut(s)	298, 322, 1118
HinP1I	GCGC	3 cut(s)	298, 322, 1118
HincII	GTYRAC	2 cut(s)	379, 1569
HindII	GTYRAC	2 cut(s)	379, 1569
HindIII	AAGCTT	1 cut(s)	821
HinfI	GANTC	8 cut(s)	503, 733, 955, 1440, 1756, 1928, 1937, 1982
HpaII	CCGG	6 cut(s)	186, 228, 410, 538, 830, 2237
HphI	GGTGA	4 cut(s)	73, 226, 235, 1274
Hpy166II	GTNNAC	9 cut(s)	379, 636, 707, 1195, 1569, 1616, 1741, 1906, 1978
Hpy8I	GTNNAC	9 cut(s)	379, 636, 707, 1195, 1569, 1616, 1741, 1906, 1978
Hpy99I	CGWCG	3 cut(s)	227, 840, 1308
HpyCH4III	ACNGT	6 cut(s)	582, 932, 1591, 1999, 2052, 2074
HpyF10VI	GCNNNNNNNGC	9 cut(s)	25, 195, 201, 288, 297, 304, 313, 368, 1651
HpyF3I	CTNAG	8 cut(s)	302, 423, 639, 819, 900, 951, 1766, 2016
Hsp92I	GRCGYC	3 cut(s)	102, 225, 1118
HspAI	GCGC	3 cut(s)	298, 322, 1118
KasI	GGCGCC	1 cut(s)	1117
KroI	GCCGGC	1 cut(s)	227
KroNI	GCCGGC	1 cut(s)	229
Ksp22I	TGATCA	1 cut(s)	1726
Kzo9I	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
LguI	GCTCTTC	1 cut(s)	1356
Lsp1109I	GCAGC	7 cut(s)	28, 176, 207, 358, 1034, 1774, 1976
LweI	GCATC	6 cut(s)	358, 385, 886, 1039, 1996, 2251
MabI	ACCWGGT	1 cut(s)	1197
MaeI	CTAG	6 cut(s)	20, 41, 585, 804, 1031, 1500
MaeIII	GTNAC	2 cut(s)	1201, 1826
MalI	GATC	6 cut(s)	435, 653, 1175, 1369, 1677, 1728
MboI	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
MfeI	CAATTG	1 cut(s)	1112
MflI	RGATCY	1 cut(s)	1675
MhlI	GDGCHC	9 cut(s)	135, 216, 417, 866, 964, 1144, 1197, 1743, 2235
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	1118
MlyI	GAGTC	4 cut(s)	512, 949, 1931, 1991
MmeI	TCCRAC	1 cut(s)	1653
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	1 cut(s)	227
MroXI	GAANNNNTTC	1 cut(s)	1350
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	7 cut(s)	401, 791, 815, 1107, 1532, 2064, 2222
MslI	CAYNNNNRTG	2 cut(s)	332, 1062
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	1474, 1967
MspI	CCGG	6 cut(s)	186, 228, 410, 538, 830, 2237
MspR9I	CCNGG	7 cut(s)	186, 830, 831, 1122, 1199, 1889, 2237
MunI	CAATTG	1 cut(s)	1112
Mva1269I	GAATGC	1 cut(s)	1275
MvaI	CCWGG	3 cut(s)	1122, 1199, 1889
MvnI	CGCG	1 cut(s)	324
MwoI	GCNNNNNNNGC	9 cut(s)	25, 195, 201, 288, 297, 304, 313, 368, 1651
NaeI	GCCGGC	1 cut(s)	229
NarI	GGCGCC	1 cut(s)	1118
NciI	CCSGG	4 cut(s)	186, 830, 831, 2237
NdeII	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
NgoMIV	GCCGGC	1 cut(s)	227
NheI	GCTAGC	1 cut(s)	19
NlaIV	GGNNCC	8 cut(s)	94, 308, 408, 865, 916, 1119, 1677, 2054
NmeAIII	GCCGAG	2 cut(s)	145, 396
NmuCI	GTSAC	2 cut(s)	1201, 1826
NspI	RCATGY	1 cut(s)	512
NspV	TTCGAA	1 cut(s)	1898
PagI	TCATGA	2 cut(s)	964, 1155
PaqCI	CACCTGC	1 cut(s)	1726
PciSI	GCTCTTC	1 cut(s)	1356
PcsI	WCGNNNNNNNCGW	2 cut(s)	273, 832
PctI	GAATGC	1 cut(s)	1275
PdiI	GCCGGC	1 cut(s)	229
PdmI	GAANNNNTTC	1 cut(s)	1350
PfeI	GAWTC	4 cut(s)	733, 1440, 1756, 1928
PflFI	GACNNNGTC	1 cut(s)	1910
PkrI	GCNGC	9 cut(s)	18, 191, 194, 197, 290, 348, 1024, 1789, 1966
PleI	GAGTC	4 cut(s)	511, 949, 1931, 1990
PluTI	GGCGCC	1 cut(s)	1121
PpsI	GAGTC	4 cut(s)	511, 949, 1931, 1990
PshBI	ATTAAT	1 cut(s)	1532
PsiI	TTATAA	1 cut(s)	2036
Psp124BI	GAGCTC	3 cut(s)	135, 417, 1144
Psp6I	CCWGG	3 cut(s)	1120, 1197, 1887
PspGI	CCWGG	3 cut(s)	1120, 1197, 1887
PspN4I	GGNNCC	8 cut(s)	94, 308, 408, 865, 916, 1119, 1677, 2054
PspPI	GGNCC	5 cut(s)	126, 306, 914, 1220, 2052
PstI	CTGCAG	1 cut(s)	1966
PsuI	RGATCY	1 cut(s)	1675
PsyI	GACNNNGTC	1 cut(s)	1910
PvuII	CAGCTG	2 cut(s)	1474, 1967
RsaI	GTAC	2 cut(s)	116, 1779
RsaNI	GTAC	2 cut(s)	115, 1778
RseI	CAYNNNNRTG	2 cut(s)	332, 1062
SacI	GAGCTC	3 cut(s)	135, 417, 1144
SapI	GCTCTTC	1 cut(s)	1356
SaqAI	TTAA	7 cut(s)	401, 791, 815, 1107, 1532, 2064, 2222
SatI	GCNGC	9 cut(s)	17, 190, 193, 196, 289, 347, 1023, 1788, 1965
Sau3AI	GATC	6 cut(s)	433, 651, 1173, 1367, 1675, 1726
Sau96I	GGNCC	5 cut(s)	126, 306, 914, 1220, 2052
SchI	GAGTC	4 cut(s)	512, 949, 1931, 1991
ScrFI	CCNGG	7 cut(s)	186, 830, 831, 1122, 1199, 1889, 2237
SduI	GDGCHC	9 cut(s)	135, 216, 417, 866, 964, 1144, 1197, 1743, 2235
SexAI	ACCWGGT	1 cut(s)	1197
SfaNI	GCATC	6 cut(s)	358, 385, 886, 1039, 1996, 2251
SfcI	CTRYAG	2 cut(s)	885, 1962
SfoI	GGCGCC	1 cut(s)	1119
SfuI	TTCGAA	1 cut(s)	1898
SinI	GGWCC	3 cut(s)	126, 1220, 2052
SmaI	CCCGGG	1 cut(s)	831
SmiMI	CAYNNNNRTG	2 cut(s)	332, 1062
SmlI	CTYRAG	3 cut(s)	350, 1939, 2210
SmoI	CTYRAG	3 cut(s)	350, 1939, 2210
SspDI	GGCGCC	1 cut(s)	1117
SspI	AATATT	1 cut(s)	440
SspMI	CTAG	6 cut(s)	20, 41, 585, 804, 1031, 1500
SstI	GAGCTC	3 cut(s)	135, 417, 1144
StyD4I	CCNGG	7 cut(s)	184, 828, 829, 1120, 1197, 1887, 2235
StyI	CCWWGG	3 cut(s)	6, 40, 261
TaaI	ACNGT	6 cut(s)	582, 932, 1591, 1999, 2052, 2074
TaqI	TCGA	6 cut(s)	108, 252, 1370, 1719, 1813, 1898
TatI	WGTACW	1 cut(s)	1777
TauI	GCSGC	2 cut(s)	195, 291
TfiI	GAWTC	4 cut(s)	733, 1440, 1756, 1928
Tru1I	TTAA	7 cut(s)	401, 791, 815, 1107, 1532, 2064, 2222
Tru9I	TTAA	7 cut(s)	401, 791, 815, 1107, 1532, 2064, 2222
TscAI	CASTG	4 cut(s)	643, 935, 1835, 2077
TseFI	GTSAC	2 cut(s)	1201, 1826
TseI	GCWGC	7 cut(s)	16, 189, 195, 346, 1022, 1787, 1964
Tsp45I	GTSAC	2 cut(s)	1201, 1826
TspMI	CCCGGG	1 cut(s)	829
TspRI	CASTG	4 cut(s)	643, 935, 1835, 2077
Tth111I	GACNNNGTC	1 cut(s)	1910
VneI	GTGCAC	2 cut(s)	1193, 1739
VpaK11BI	GGWCC	3 cut(s)	126, 1220, 2052
VspI	ATTAAT	1 cut(s)	1532
XapI	RAATTY	6 cut(s)	479, 738, 1146, 1411, 1893, 2038
XceI	RCATGY	1 cut(s)	512
XmaI	CCCGGG	1 cut(s)	829
XmaJI	CCTAGG	1 cut(s)	40
XmiI	GTMKAC	1 cut(s)	1905
XmnI	GAANNNNTTC	1 cut(s)	1350
XspI	CTAG	6 cut(s)	20, 41, 585, 804, 1031, 1500

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.