MD01G1027000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011893
Physical Location & Seq
Forward (+)
9939857 .. 9942519
2663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1027000.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGAGTAGTGATAATTCGACCGATGGGTTTTGCAAATCCCGACGGGTTCTCGGTGATTTGACCAACCGGCCCATAAAAAGGGGGTTTTCGATGATTTCAGCTGATTCGGGTACTAAATCTCTAAATGCAGTTGGTAAAAATGTTGATTGTGAACATAGGATTTCAAAAGTTGCAAAGCAAGTGTTTTTAGGGGTTGAGGATTTAGTAAGAGAGAAATGCGAGAAAAAAACCGGAGTAGATTGTAACCCTGAAGCTTTATCTTCGCCAAAGGGCACTCAAGAATCTGTGTTTTCACCTACTTATAGTGACATTGATGATTCCCCAAATGACAACAGTGATTCTGTTACGTTGACTATGTCTAATGATATCGGGGAAAAGTCCAGTGACAGTGGAGCTAATGCTAGCAGTGTTTTAGAAGTCGGCGATGCATTGATGGATAATGCCTCGTCCATTGCTTCAATTGCAAAATGCTCAGGAATGTGCAAGAATGACTGTTGTGGTGGTGGGGAGAAATGTCAATACAAGAAAGCGGGACATATTTCAGAAATTAATCAAAGCGTTCCTCCATATGAAGGATTAGTCACTGTTGTTCATGGTAATGACGAAAAGGGTCTTGGTGTTGGTAAAGTGGCAGCAACCAAATATGGTTCTAATGAGTGGTCGATGTTGCCTAGGTCGCAAAGTTGCTCGAAAGTCCATGAATTTGAGAAGTTGGAAAGATGCACTGCTCTTAAGGGTGGTGGCAGTGCTGATTTGAGTGTAGGTGATGACTTGCTCAAAGATTGCTCTTGTTCATTTTGCTTAAAAGCTGCTCACATCTTGTCGGACCTCCAATACCAGGATATCAAGGGTCGAATTGCTGCACTGAAGAAGAGTAAAAAAGAAGCGAACATATTGGTTGAGAAAAGTGTCAAGGGAAAAGAGAGTGATACCAAGGGACGACTACACCCAAACAAAGTGTCAAAACTTGAGAATGATCTCTCAAGTCAGTGGAGATCACTCTTTCTTAACATGGAGGATGTGCTTGTTCATGAAAGCAACCATCTTCAAGACAGTTATGTCACGCTAAAAAAATTGAGGGACAACTGCAAGACAGAGCTTGAAGTTACCAGTGGAATGCCTTACGAGAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

40.91

Weight (kDa)

6.28

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10110 AT5G65120 AT5G65120
fragaria_vesca FvH4_1g21240
malus_domestica MD01G1027000.v1.1 MD15G1327000.v1.1
prunus_persica Prupe.6G182000_v2.0.a1 Prupe.6G182000_v2.0.a1
pyrus_communis pycom01g05490 pycom395g00050
rosa_chinensis RchiOBHm_Chr2g0115081
rosa_laevigata RLG00000018155
rosa_multiflora Rmu_sc0002784.1_g000001
rosa_roxburghii Rroxscaffold_2G00130550
rosa_rugosa Rorug02G0193200
rosa_samantha Rh2AG251700 Rh2BG261200 Rh2DG259800
rosa_wichuraiana Rw2G019630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1058
AciI CCGC 1 cut(s) 530
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 2 cut(s) 270, 887
AfaI GTAC 1 cut(s) 112
AfiI CCNNNNNNNGG 3 cut(s) 78, 572, 838
AflII CTTAAG 1 cut(s) 731
AgsI TTSAA 4 cut(s) 165, 459, 1049, 1103
AjnI CCWGG 1 cut(s) 837
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AluBI AGCT 5 cut(s) 101, 254, 395, 809, 1099
AluI AGCT 5 cut(s) 101, 254, 395, 809, 1099
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 3 cut(s) 632, 809, 860
ApoI RAATTY 1 cut(s) 701
ArsI GACNNNNNNTTYG 2 cut(s) 944, 976
AseI ATTAAT 1 cut(s) 549
AspA2I CCTAGG 1 cut(s) 671
AspS9I GGNCC 2 cut(s) 69, 826
AsuHPI GGTGA 3 cut(s) 65, 285, 776
AsuNHI GCTAGC 1 cut(s) 401
AvaII GGWCC 1 cut(s) 826
AvrII CCTAGG 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 275
BbvI GCAGC 3 cut(s) 644, 796, 847
BccI CCATC 3 cut(s) 17, 427, 1050
BciT130I CCWGG 1 cut(s) 839
BfaI CTAG 2 cut(s) 402, 672
BfrI CTTAAG 1 cut(s) 731
BisI GCNGC 3 cut(s) 633, 810, 861
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 3 cut(s) 634, 811, 862
Bme1390I CCNGG 1 cut(s) 839
Bme18I GGWCC 1 cut(s) 826
BmgT120I GGNCC 2 cut(s) 69, 826
BmrFI CCNGG 1 cut(s) 839
BmsI GCATC 2 cut(s) 415, 710
BmtI GCTAGC 1 cut(s) 405
Bpu10I CCTNAGC 1 cut(s) 472
BpuEI CTTGAG 3 cut(s) 261, 967, 989
BsaJI CCNNGG 2 cut(s) 671, 933
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 572, 838
Bse118I RCCGGY 1 cut(s) 66
Bse1I ACTGG 2 cut(s) 381, 1110
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 1 cut(s) 839
BseDI CCNNGG 2 cut(s) 671, 933
BseGI GGATG 1 cut(s) 1024
BseLI CCNNNNNNNGG 3 cut(s) 78, 572, 838
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 2 cut(s) 381, 1110
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 3 cut(s) 644, 796, 847
BsgI GTGCAG 1 cut(s) 846
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 21
BsiSI CCGG 2 cut(s) 67, 231
BslFI GGGAC 3 cut(s) 546, 951, 1094
BslI CCNNNNNNNGG 3 cut(s) 78, 572, 838
BsmFI GGGAC 3 cut(s) 546, 951, 1094
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 2 cut(s) 976, 995
BspACI CCGC 1 cut(s) 530
BspANI GGCC 1 cut(s) 70
BspCNI CTCAG 1 cut(s) 485
BspHI TCATGA 1 cut(s) 1030
BspOI GCTAGC 1 cut(s) 405
BspTI CTTAAG 1 cut(s) 731
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 66
BsrI ACTGG 2 cut(s) 381, 1110
BssAI RCCGGY 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 671, 933
BssMI GATC 2 cut(s) 976, 995
BssT1I CCWWGG 2 cut(s) 671, 933
Bst2UI CCWGG 1 cut(s) 839
Bst4CI ACNGT 5 cut(s) 335, 389, 494, 586, 1055
Bst6I CTCTTC 1 cut(s) 866
BstAFI CTTAAG 1 cut(s) 731
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 1 cut(s) 472
BstF5I GGATG 1 cut(s) 1024
BstKTI GATC 2 cut(s) 979, 998
BstMBI GATC 2 cut(s) 976, 995
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 2 cut(s) 461, 676
BstNI CCWGG 1 cut(s) 839
BstSCI CCNGG 1 cut(s) 837
BstSLI GKGCMC 1 cut(s) 275
BstV1I GCAGC 3 cut(s) 644, 796, 847
BsuRI GGCC 1 cut(s) 70
BtgZI GCGATG 1 cut(s) 438
BtsCI GGATG 1 cut(s) 1024
BtsI GCAGTG 3 cut(s) 412, 723, 751
Cac8I GCNNGC 1 cut(s) 403
CciI TCATGA 1 cut(s) 1030
Cfr10I RCCGGY 1 cut(s) 66
Cfr13I GGNCC 2 cut(s) 69, 826
Csp6I GTAC 1 cut(s) 111
CviAII CATG 4 cut(s) 593, 698, 1012, 1031
CviJI RGCY 6 cut(s) 70, 101, 254, 395, 809, 1099
CviKI_1 RGCY 6 cut(s) 70, 101, 254, 395, 809, 1099
CviQI GTAC 1 cut(s) 111
DdeI CTNAG 1 cut(s) 472
DpnI GATC 2 cut(s) 978, 997
DpnII GATC 2 cut(s) 976, 995
DrdI GACNNNNNNGTC 1 cut(s) 1058
DseDI GACNNNNNNGTC 1 cut(s) 1058
Eam1104I CTCTTC 1 cut(s) 866
EarI CTCTTC 1 cut(s) 866
Eco130I CCWWGG 2 cut(s) 671, 933
Eco32I GATATC 2 cut(s) 367, 844
Eco47I GGWCC 1 cut(s) 826
Eco57I CTGAAG 2 cut(s) 270, 887
EcoRII CCWGG 1 cut(s) 837
EcoRV GATATC 2 cut(s) 367, 844
EcoT14I CCWWGG 2 cut(s) 671, 933
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 2 cut(s) 671, 933
FaeI CATG 4 cut(s) 596, 701, 1015, 1034
FaqI GGGAC 3 cut(s) 546, 951, 1094
FatI CATG 4 cut(s) 592, 697, 1011, 1030
FauI CCCGC 1 cut(s) 523
FauNDI CATATG 1 cut(s) 568
Fnu4HI GCNGC 3 cut(s) 633, 810, 861
FokI GGATG 1 cut(s) 1031
Fsp4HI GCNGC 3 cut(s) 633, 810, 861
FspBI CTAG 2 cut(s) 402, 672
GluI GCNGC 3 cut(s) 633, 810, 861
HaeIII GGCC 1 cut(s) 70
HapII CCGG 2 cut(s) 67, 231
Hin1II CATG 4 cut(s) 596, 701, 1015, 1034
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 4 cut(s) 104, 281, 317, 338
HpaII CCGG 2 cut(s) 67, 231
HphI GGTGA 3 cut(s) 65, 285, 776
Hpy166II GTNNAC 2 cut(s) 152, 351
Hpy188I TCNGA 2 cut(s) 544, 826
Hpy188III TCNNGA 5 cut(s) 39, 278, 474, 1031, 1049
Hpy8I GTNNAC 2 cut(s) 152, 351
Hpy99I CGWCG 1 cut(s) 45
HpyAV CCTTC 1 cut(s) 566
HpyCH4III ACNGT 5 cut(s) 335, 389, 494, 586, 1055
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 9 cut(s) 33, 128, 173, 428, 464, 483, 723, 863, 1089
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 676
HpyF3I CTNAG 1 cut(s) 472
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 4 cut(s) 596, 701, 1015, 1034
Kzo9I GATC 2 cut(s) 976, 995
LmnI GCTCC 1 cut(s) 392
LpnPI CCDG 8 cut(s) 80, 244, 261, 394, 459, 824, 851, 1123
Lsp1109I GCAGC 3 cut(s) 644, 796, 847
LweI GCATC 2 cut(s) 415, 710
MaeI CTAG 2 cut(s) 402, 672
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 7 cut(s) 242, 305, 343, 383, 580, 1060, 1105
MalI GATC 2 cut(s) 978, 997
MboI GATC 2 cut(s) 976, 995
MboII GAAGA 4 cut(s) 252, 880, 883, 1037
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 275
MluCI AATT 6 cut(s) 13, 459, 546, 701, 855, 1073
MmeI TCCRAC 2 cut(s) 693, 804
MnlI CCTC 6 cut(s) 190, 454, 573, 839, 1009, 1071
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 4 cut(s) 549, 732, 803, 1008
MslI CAYNNNNRTG 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 101
MspCI CTTAAG 1 cut(s) 731
MspI CCGG 2 cut(s) 67, 231
MspR9I CCNGG 1 cut(s) 839
MunI CAATTG 1 cut(s) 459
Mva1269I GAATGC 1 cut(s) 1122
MvaI CCWGG 1 cut(s) 839
MwoI GCNNNNNNNGC 2 cut(s) 461, 676
NdeI CATATG 1 cut(s) 568
NdeII GATC 2 cut(s) 976, 995
NheI GCTAGC 1 cut(s) 401
NlaIII CATG 4 cut(s) 596, 701, 1015, 1034
NmuCI GTSAC 4 cut(s) 305, 383, 580, 1060
NsiI ATGCAT 1 cut(s) 430
PagI TCATGA 1 cut(s) 1030
PctI GAATGC 1 cut(s) 1122
PfeI GAWTC 4 cut(s) 104, 281, 317, 338
PflFI GACNNNGTC 1 cut(s) 355
PkrI GCNGC 3 cut(s) 634, 811, 862
PshBI ATTAAT 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 837
PspGI CCWGG 1 cut(s) 837
PspPI GGNCC 2 cut(s) 69, 826
PsyI GACNNNGTC 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 4 cut(s) 549, 732, 803, 1008
SatI GCNGC 3 cut(s) 633, 810, 861
Sau3AI GATC 2 cut(s) 976, 995
Sau96I GGNCC 2 cut(s) 69, 826
ScrFI CCNGG 1 cut(s) 839
SduI GDGCHC 1 cut(s) 275
SfaNI GCATC 2 cut(s) 415, 710
SinI GGWCC 1 cut(s) 826
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 4 cut(s) 276, 731, 968, 982
SmoI CTYRAG 4 cut(s) 276, 731, 968, 982
Sse9I AATT 6 cut(s) 13, 459, 546, 701, 855, 1073
SsiI CCGC 1 cut(s) 530
SspMI CTAG 2 cut(s) 402, 672
StyD4I CCNGG 1 cut(s) 837
StyI CCWWGG 2 cut(s) 671, 933
TaaI ACNGT 5 cut(s) 335, 389, 494, 586, 1055
TaiI ACGT 1 cut(s) 350
TaqI TCGA 5 cut(s) 17, 89, 662, 689, 853
TaqII GACCGA 1 cut(s) 35
TasI AATT 6 cut(s) 13, 459, 546, 701, 855, 1073
TfiI GAWTC 4 cut(s) 104, 281, 317, 338
Tru1I TTAA 4 cut(s) 549, 732, 803, 1008
Tru9I TTAA 4 cut(s) 549, 732, 803, 1008
TseFI GTSAC 4 cut(s) 305, 383, 580, 1060
TseI GCWGC 3 cut(s) 632, 809, 860
Tsp45I GTSAC 4 cut(s) 305, 383, 580, 1060
TspDTI ATGAA 6 cut(s) 581, 585, 714, 783, 1019, 1047
Tth111I GACNNNGTC 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 731
VpaK11BI GGWCC 1 cut(s) 826
VspI ATTAAT 1 cut(s) 549
XapI RAATTY 1 cut(s) 701
XmaJI CCTAGG 1 cut(s) 671
XspI CTAG 2 cut(s) 402, 672
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.