MD15G1327000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011893
Physical Location & Seq
Reverse (-)
35315653 .. 35318409
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1327000.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGCTTAATTGGCTTGATTATATTTCGATTTTGTTTATCCTTTTGCAACCGAATTGCACTATGAGTAGTGATAATTCAACCGATGGGTCTTGCAAATCCCGACGGGTTCTCAGTGATTTGACCAACCGGCCCATAAAAAGGGGGTTTTCGATGGTTTCAGCTGCTTCGGGTGCTAAATCTCGAGATGAGTTTGGTAAAATTGTTGATTTGGAAAATAGGGGTTCGAAATTTGCAAAGCAATTGTCTTTAGGGGTTGAGGATTTAGTAGGAGAGAAATGCAAGACCAAAACTGGAGCAGATTGTAACCCTGAAGCTTTGTCTTCGCCTAAGGGCACACAGGAATCAGTGTTTTCGCCTACTTATAGTGACATTGATGATTCGTCGAATGACAACAGCGATTCTGTTACCTTGACTATGTCAAATGATATGGGGGAAAGGTCCAGTGACAGTGGAGCTAATGCGGGCAGTGCTCTAGAAGTCAGTGATGCATTGATGGATAATTGCTCGTCCATTGCTTCAATTTCAAAATGCTCAGGAATGAGTAAGAAAGACTGTTGTGGTGGTGGGGGGAAATGTCAATATGATAAACCCGGACATATTTCAGAAGTTAATCAAAGCGGTCCTCTATATGAAGGATTAGTCACTGTTGTTCGCGGTAATAACGAAAAGGATCTTGGAGGTGGTAAAGTGGCAACAACCAAATATGATTCTAATGAGTGGTCGAGGTTGCCTAGGTCGCAAAGTTGCTCAAAATCCCACGAATTCAAAAAGTTGGAAAGATGCACGACTCTTAAAGGTGGTGGCAGTGCTGATTTGAATGTAGGTGATGACTTGCTCAAAGATTGCTCTTGTTCGTTTTGCTTGAAAGCTGCTCACATCTTGTCGGATCTCCAATACCAGGATATCAAGGGTCGAATTGCTGCATTGAAGAGGAGTAAAAAAGAAGCGAGCGTATTGGTTGAGAAAAGTGTCAGGGGGAAGGAGATTAATACTAAGGGCCAACTACACCCAAACAAAGCGTCAAAAAGTGAATATGATCTCTCGAGTCAGTGGAGATCACTCTTTCTTAACATGAAGGATGTGCTTGTTCATGAAAGCAACCATCTTGAAGACTGTTATGTCGCACTAAAAAATTTGAGGGACAACTGCAAGACGGAACTTGAAATTACCAGTGGAATGCCTTCCGAGAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

399

Amino Acids

43.18

Weight (kDa)

6.61

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10110 AT5G65120 AT5G65120
fragaria_vesca FvH4_1g21240
malus_domestica MD01G1027000.v1.1 MD15G1327000.v1.1
prunus_persica Prupe.6G182000_v2.0.a1 Prupe.6G182000_v2.0.a1
pyrus_communis pycom01g05490 pycom395g00050
rosa_chinensis RchiOBHm_Chr2g0115081
rosa_laevigata RLG00000018155
rosa_multiflora Rmu_sc0002784.1_g000001
rosa_roxburghii Rroxscaffold_2G00130550
rosa_rugosa Rorug02G0193200
rosa_samantha Rh2AG251700 Rh2BG261200 Rh2DG259800
rosa_wichuraiana Rw2G019630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1118
AccII CGCG 1 cut(s) 654
AciI CCGC 3 cut(s) 461, 618, 654
AclWI GGATC 2 cut(s) 678, 894
AcsI RAATTY 3 cut(s) 227, 761, 1132
AcuI CTGAAG 1 cut(s) 330
AfiI CCNNNNNNNGG 2 cut(s) 138, 898
AgsI TTSAA 9 cut(s) 78, 519, 525, 766, 817, 865, 928, 1109, 1163
AjnI CCWGG 1 cut(s) 897
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AluBI AGCT 4 cut(s) 161, 314, 455, 869
AluI AGCT 4 cut(s) 161, 314, 455, 869
Alw21I GWGCWC 1 cut(s) 472
AlwI GGATC 2 cut(s) 678, 894
Ama87I CYCGRG 2 cut(s) 180, 1042
AoxI GGCC 2 cut(s) 128, 997
ApeKI GCWGC 3 cut(s) 161, 869, 920
ApoI RAATTY 3 cut(s) 227, 761, 1132
ArsI GACNNNNNNTTYG 4 cut(s) 227, 259, 1004, 1036
AseI ATTAAT 1 cut(s) 987
Asp700I GAANNNNTTC 1 cut(s) 1180
AspA2I CCTAGG 1 cut(s) 731
AspS9I GGNCC 4 cut(s) 129, 438, 620, 997
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 1 cut(s) 836
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 2 cut(s) 180, 1042
AvaII GGWCC 2 cut(s) 438, 620
AvrII CCTAGG 1 cut(s) 731
AxyI CCTNAGG 1 cut(s) 327
BaeGI GKGCMC 1 cut(s) 335
BbsI GAAGAC 2 cut(s) 312, 1116
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 3 cut(s) 148, 856, 907
BccI CCATC 4 cut(s) 77, 145, 487, 1110
BciT130I CCWGG 1 cut(s) 899
BcnI CCSGG 1 cut(s) 591
BfaI CTAG 2 cut(s) 473, 732
BisI GCNGC 3 cut(s) 162, 870, 921
BlnI CCTAGG 1 cut(s) 731
BlsI GCNGC 3 cut(s) 163, 871, 922
Bme1390I CCNGG 2 cut(s) 591, 899
Bme18I GGWCC 2 cut(s) 438, 620
BmeT110I CYCGRG 2 cut(s) 180, 1042
BmgT120I GGNCC 4 cut(s) 129, 438, 620, 997
BmrFI CCNGG 2 cut(s) 591, 899
BmsI GCATC 2 cut(s) 475, 770
BpiI GAAGAC 2 cut(s) 312, 1116
BpmI CTGGAG 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 532
Bpu14I TTCGAA 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 591
BsaJI CCNNGG 1 cut(s) 731
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 898
Bse118I RCCGGY 1 cut(s) 126
Bse1I ACTGG 3 cut(s) 295, 441, 1170
Bse21I CCTNAGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 899
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 1 cut(s) 1084
BseLI CCNNNNNNNGG 2 cut(s) 138, 898
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 124, 546
BseNI ACTGG 3 cut(s) 295, 441, 1170
BseRI GAGGAG 1 cut(s) 946
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 3 cut(s) 148, 856, 907
Bsh1236I CGCG 1 cut(s) 654
BshFI GGCC 2 cut(s) 130, 999
BsiHKAI GWGCWC 1 cut(s) 472
BsiHKCI CYCGRG 2 cut(s) 180, 1042
BsiSI CCGG 2 cut(s) 127, 591
BslFI GGGAC 1 cut(s) 1154
BslI CCNNNNNNNGG 2 cut(s) 138, 898
BsmFI GGGAC 1 cut(s) 1154
BsmI GAATGC 1 cut(s) 1182
BsnI GGCC 2 cut(s) 130, 999
BsoBI CYCGRG 2 cut(s) 180, 1042
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 335, 472
Bsp143I GATC 4 cut(s) 670, 886, 1036, 1055
BspACI CCGC 3 cut(s) 461, 618, 654
BspANI GGCC 2 cut(s) 130, 999
BspCNI CTCAG 2 cut(s) 123, 545
BspFNI CGCG 1 cut(s) 654
BspHI TCATGA 1 cut(s) 1090
BspPI GGATC 2 cut(s) 678, 894
BspT104I TTCGAA 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 510
BsrFI RCCGGY 1 cut(s) 126
BsrI ACTGG 3 cut(s) 295, 441, 1170
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 731
BssMI GATC 4 cut(s) 670, 886, 1036, 1055
BssT1I CCWWGG 1 cut(s) 731
Bst2UI CCWGG 1 cut(s) 899
Bst4CI ACNGT 4 cut(s) 449, 554, 646, 1115
Bst6I CTCTTC 1 cut(s) 923
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 463, 949
BstDEI CTNAG 4 cut(s) 110, 327, 532, 993
BstF5I GGATG 1 cut(s) 1084
BstFNI CGCG 1 cut(s) 654
BstKTI GATC 4 cut(s) 673, 889, 1039, 1058
BstMBI GATC 4 cut(s) 670, 886, 1036, 1055
BstMWI GCNNNNNNNGC 4 cut(s) 10, 170, 467, 736
BstNI CCWGG 1 cut(s) 899
BstSCI CCNGG 2 cut(s) 589, 897
BstSLI GKGCMC 1 cut(s) 335
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 3 cut(s) 148, 856, 907
BstV2I GAAGAC 2 cut(s) 312, 1116
BstX2I RGATCY 2 cut(s) 670, 886
BstYI RGATCY 2 cut(s) 670, 886
Bsu36I CCTNAGG 1 cut(s) 327
BsuRI GGCC 2 cut(s) 130, 999
BtsCI GGATG 1 cut(s) 1084
BtsI GCAGTG 2 cut(s) 472, 811
Cac8I GCNNGC 2 cut(s) 463, 949
CciI TCATGA 1 cut(s) 1090
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 4 cut(s) 129, 438, 620, 997
CseI GACGC 1 cut(s) 1008
CviAII CATG 2 cut(s) 1072, 1091
CviJI RGCY 7 cut(s) 13, 130, 161, 314, 455, 869, 999
CviKI_1 RGCY 7 cut(s) 13, 130, 161, 314, 455, 869, 999
DdeI CTNAG 4 cut(s) 110, 327, 532, 993
DpnI GATC 4 cut(s) 672, 888, 1038, 1057
DpnII GATC 4 cut(s) 670, 886, 1036, 1055
DrdI GACNNNNNNGTC 1 cut(s) 1118
DseDI GACNNNNNNGTC 1 cut(s) 1118
Eam1104I CTCTTC 1 cut(s) 923
EarI CTCTTC 1 cut(s) 923
Eco130I CCWWGG 1 cut(s) 731
Eco32I GATATC 1 cut(s) 904
Eco47I GGWCC 2 cut(s) 438, 620
Eco57I CTGAAG 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 327
Eco88I CYCGRG 2 cut(s) 180, 1042
EcoRI GAATTC 1 cut(s) 761
EcoRII CCWGG 1 cut(s) 897
EcoRV GATATC 1 cut(s) 904
EcoT14I CCWWGG 1 cut(s) 731
EcoT22I ATGCAT 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 731
FaeI CATG 2 cut(s) 1075, 1094
FaqI GGGAC 1 cut(s) 1154
FatI CATG 2 cut(s) 1071, 1090
FauI CCCGC 1 cut(s) 454
Fnu4HI GCNGC 3 cut(s) 162, 870, 921
FokI GGATG 1 cut(s) 1091
Fsp4HI GCNGC 3 cut(s) 162, 870, 921
FspBI CTAG 2 cut(s) 473, 732
GluI GCNGC 3 cut(s) 162, 870, 921
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 2 cut(s) 130, 999
HapII CCGG 2 cut(s) 127, 591
HgaI GACGC 1 cut(s) 1008
Hin1II CATG 2 cut(s) 1075, 1094
HindIII AAGCTT 1 cut(s) 312
HinfI GANTC 6 cut(s) 341, 377, 398, 707, 787, 1045
HpaII CCGG 2 cut(s) 127, 591
HphI GGTGA 1 cut(s) 836
Hpy188I TCNGA 3 cut(s) 604, 886, 1186
Hpy188III TCNNGA 8 cut(s) 99, 180, 182, 473, 534, 1042, 1091, 1106
Hpy99I CGWCG 2 cut(s) 105, 385
HpyAV CCTTC 4 cut(s) 626, 973, 1069, 1191
HpyCH4III ACNGT 4 cut(s) 449, 554, 646, 1115
HpyCH4V TGCA 9 cut(s) 46, 57, 93, 233, 279, 488, 783, 923, 1149
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 170, 467, 736
HpyF3I CTNAG 4 cut(s) 110, 327, 532, 993
Hsp92II CATG 2 cut(s) 1075, 1094
Kzo9I GATC 4 cut(s) 670, 886, 1036, 1055
LmnI GCTCC 2 cut(s) 293, 452
Lsp1109I GCAGC 3 cut(s) 148, 856, 907
LweI GCATC 2 cut(s) 475, 770
MaeI CTAG 2 cut(s) 473, 732
MaeIII GTNAC 5 cut(s) 302, 365, 403, 443, 640
MalI GATC 4 cut(s) 672, 888, 1038, 1057
MboI GATC 4 cut(s) 670, 886, 1036, 1055
MboII GAAGA 3 cut(s) 312, 940, 1121
MfeI CAATTG 1 cut(s) 239
MflI RGATCY 2 cut(s) 670, 886
MhlI GDGCHC 2 cut(s) 335, 472
MlyI GAGTC 2 cut(s) 781, 1054
MmeI TCCRAC 2 cut(s) 753, 864
MnlI CCTC 6 cut(s) 250, 633, 671, 717, 924, 1131
Mph1103I ATGCAT 1 cut(s) 490
MroXI GAANNNNTTC 1 cut(s) 1180
MseI TTAA 5 cut(s) 6, 609, 792, 987, 1068
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 127, 591
MspR9I CCNGG 2 cut(s) 591, 899
MunI CAATTG 1 cut(s) 239
Mva1269I GAATGC 1 cut(s) 1182
MvaI CCWGG 1 cut(s) 899
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 4 cut(s) 10, 170, 467, 736
NciI CCSGG 1 cut(s) 591
NdeII GATC 4 cut(s) 670, 886, 1036, 1055
NlaIII CATG 2 cut(s) 1075, 1094
NmuCI GTSAC 3 cut(s) 365, 443, 640
NsiI ATGCAT 1 cut(s) 490
NspV TTCGAA 1 cut(s) 224
PaeR7I CTCGAG 2 cut(s) 180, 1042
PagI TCATGA 1 cut(s) 1090
PctI GAATGC 1 cut(s) 1182
PdmI GAANNNNTTC 1 cut(s) 1180
PfeI GAWTC 4 cut(s) 341, 377, 398, 707
PflFI GACNNNGTC 1 cut(s) 415
PkrI GCNGC 3 cut(s) 163, 871, 922
PleI GAGTC 2 cut(s) 781, 1053
PpsI GAGTC 2 cut(s) 781, 1053
PshBI ATTAAT 1 cut(s) 987
Psp6I CCWGG 1 cut(s) 897
PspGI CCWGG 1 cut(s) 897
PspPI GGNCC 4 cut(s) 129, 438, 620, 997
PsuI RGATCY 2 cut(s) 670, 886
PsyI GACNNNGTC 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 161
SaqAI TTAA 5 cut(s) 6, 609, 792, 987, 1068
SatI GCNGC 3 cut(s) 162, 870, 921
Sau3AI GATC 4 cut(s) 670, 886, 1036, 1055
Sau96I GGNCC 4 cut(s) 129, 438, 620, 997
SchI GAGTC 2 cut(s) 781, 1054
ScrFI CCNGG 2 cut(s) 591, 899
SduI GDGCHC 2 cut(s) 335, 472
SfaNI GCATC 2 cut(s) 475, 770
Sfr274I CTCGAG 2 cut(s) 180, 1042
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 2 cut(s) 438, 620
SlaI CTCGAG 2 cut(s) 180, 1042
SmlI CTYRAG 2 cut(s) 180, 1042
SmoI CTYRAG 2 cut(s) 180, 1042
SsiI CCGC 3 cut(s) 461, 618, 654
SspMI CTAG 2 cut(s) 473, 732
StyD4I CCNGG 2 cut(s) 589, 897
StyI CCWWGG 1 cut(s) 731
TaaI ACNGT 4 cut(s) 449, 554, 646, 1115
TaqI TCGA 8 cut(s) 26, 149, 181, 224, 383, 722, 913, 1043
TfiI GAWTC 4 cut(s) 341, 377, 398, 707
Tru1I TTAA 5 cut(s) 6, 609, 792, 987, 1068
Tru9I TTAA 5 cut(s) 6, 609, 792, 987, 1068
TseFI GTSAC 3 cut(s) 365, 443, 640
TseI GCWGC 3 cut(s) 161, 869, 920
Tsp45I GTSAC 3 cut(s) 365, 443, 640
TspDTI ATGAA 4 cut(s) 645, 1079, 1088, 1107
TspGWI ACGGA 1 cut(s) 1169
Tth111I GACNNNGTC 1 cut(s) 415
VpaK11BI GGWCC 2 cut(s) 438, 620
VspI ATTAAT 1 cut(s) 987
XapI RAATTY 3 cut(s) 227, 761, 1132
XbaI TCTAGA 1 cut(s) 472
XhoI CTCGAG 2 cut(s) 180, 1042
XmaJI CCTAGG 1 cut(s) 731
XmnI GAANNNNTTC 1 cut(s) 1180
XspI CTAG 2 cut(s) 473, 732
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.