pycom01g05490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011893
Physical Location & Seq
Forward (+)
5211277 .. 5213083
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05490.4

Sequence Viewer

Length: 1119 bp
ATGAGTAGTGATAATTCGACCGATGGGTTTTACAAATCCCGACAGGTTCTCGGTGATTTGACCAACCGGCCCATAAAAAGGGGGTTTTCGATGATTTCAGCTGATTCGGGTACTAAATCTCTAAATGCAGTTGGTAAAAATGTTGATTGTGAACATAGGGGTTCAAAAGTTGCAAAGCAAGTGTTTTTAGGGGTTGAGGATTTAGTAAGAGAGAAATGCGAGAAAAAAACCGGAGTAGATTGTAACCCTGAAGCTTTATCTTCGCCAAAGGGCACTCAAGAATCTGTGTTTTCACCTACTTATAGTGACATTGATGATTCCTCGAATGACAACAGTGATTCTGTTACCTTGACGATGTCTAATGATATCGGGGAAAAGTCCAGTGACAGTGGAGCTAATGCTAGCAGTGTTTTAGAAGTTGGCGATGCATTGATGGATAATGCCTCGTCCATTGCTTCAATTTCAAAATGCTCAGGAATGTGCAAGAATGATTGTTGTGGTGGTGGGGAGAAATGTCAATACAAGAAAGTAGGACATATTTCAGAAATTAATCAAAGCGTTCCTCTATATGAAGGATTAGTCACTGTTGTTCATGGTAATGACGAAAAGGGTCTTGGTGTTGGTCAAGTGGCAGCAACCAAATATGGTTCTAATGAGTGGTCGATGTTGCCTAGGTCGCAAAGTTGCTCGAAAGTCCATGAATTTGAGAAGTTGGAAAGATGCACGGCTCTTAAGGGTGGTGGCAGTGCTGATTTGAGTGTAGGTGATGACTTGCTCAAAGATTGCTCTTGTTCGTTTTGCTTAAAAGACCTCCAATACCAGGATATCAAGGGTCGAATTGCTGCACTGAAGAAGAGTAAAAGAGAAGTGAACGTATTGGTTGAGAAAAGTGTCAAGGGAAAAGAGAGTGATACCAAGGGACAACTACACCCAAACAAAGCGTCAAAACTTGAATATGATCTCTCAAGTCAGTGGAGATCACTCTTTCTTAACATGGAGGATGTGCTTGTTCATGAAAGCAACCATCTTCAAGACAGTTATGTCACGCTAAAAAATTTGAGGGACAACTGCAAGACAGAGCTTGAAGTTACCAGTGGAAAGCCTTCCGAGAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

373

Amino Acids

40.29

Weight (kDa)

5.76

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10110 AT5G65120 AT5G65120
fragaria_vesca FvH4_1g21240
malus_domestica MD01G1027000.v1.1 MD15G1327000.v1.1
prunus_persica Prupe.6G182000_v2.0.a1 Prupe.6G182000_v2.0.a1
pyrus_communis pycom01g05490 pycom395g00050
rosa_chinensis RchiOBHm_Chr2g0115081
rosa_laevigata RLG00000018155
rosa_multiflora Rmu_sc0002784.1_g000001
rosa_roxburghii Rroxscaffold_2G00130550
rosa_rugosa Rorug02G0193200
rosa_samantha Rh2AG251700 Rh2BG261200 Rh2DG259800
rosa_wichuraiana Rw2G019630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1040
AcsI RAATTY 2 cut(s) 701, 1054
AcuI CTGAAG 2 cut(s) 270, 869
AfaI GTAC 1 cut(s) 112
AfiI CCNNNNNNNGG 2 cut(s) 78, 820
AflII CTTAAG 1 cut(s) 731
AgsI TTSAA 6 cut(s) 165, 459, 465, 953, 1031, 1085
AjnI CCWGG 1 cut(s) 819
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AluBI AGCT 4 cut(s) 101, 254, 395, 1081
AluI AGCT 4 cut(s) 101, 254, 395, 1081
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 2 cut(s) 632, 842
ApoI RAATTY 2 cut(s) 701, 1054
ArsI GACNNNNNNTTYG 2 cut(s) 926, 958
AseI ATTAAT 1 cut(s) 549
Asp700I GAANNNNTTC 1 cut(s) 1102
AspA2I CCTAGG 1 cut(s) 671
AspS9I GGNCC 1 cut(s) 69
AsuHPI GGTGA 3 cut(s) 65, 285, 776
AsuNHI GCTAGC 1 cut(s) 401
AvrII CCTAGG 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 275
BbvI GCAGC 2 cut(s) 644, 829
BccI CCATC 3 cut(s) 17, 427, 1032
BceAI ACGGC 1 cut(s) 741
BciT130I CCWGG 1 cut(s) 821
BfaI CTAG 2 cut(s) 402, 672
BfrI CTTAAG 1 cut(s) 731
BisI GCNGC 2 cut(s) 633, 843
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 2 cut(s) 634, 844
Bme1390I CCNGG 1 cut(s) 821
BmgT120I GGNCC 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 821
BmsI GCATC 2 cut(s) 415, 710
BmtI GCTAGC 1 cut(s) 405
Bpu10I CCTNAGC 1 cut(s) 472
BpuEI CTTGAG 2 cut(s) 261, 949
BsaJI CCNNGG 2 cut(s) 671, 915
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 820
Bse118I RCCGGY 1 cut(s) 66
Bse1I ACTGG 2 cut(s) 381, 1092
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 1 cut(s) 821
BseDI CCNNGG 2 cut(s) 671, 915
BseGI GGATG 1 cut(s) 1006
BseLI CCNNNNNNNGG 2 cut(s) 78, 820
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 2 cut(s) 381, 1092
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 2 cut(s) 644, 829
BsgI GTGCAG 1 cut(s) 828
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 21
BsiSI CCGG 2 cut(s) 67, 231
BslFI GGGAC 2 cut(s) 933, 1076
BslI CCNNNNNNNGG 2 cut(s) 78, 820
BsmFI GGGAC 2 cut(s) 933, 1076
BsnI GGCC 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 2 cut(s) 958, 977
BspANI GGCC 1 cut(s) 70
BspCNI CTCAG 1 cut(s) 485
BspHI TCATGA 1 cut(s) 1012
BspOI GCTAGC 1 cut(s) 405
BspTI CTTAAG 1 cut(s) 731
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 66
BsrI ACTGG 2 cut(s) 381, 1092
BssAI RCCGGY 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 671, 915
BssMI GATC 2 cut(s) 958, 977
BssT1I CCWWGG 2 cut(s) 671, 915
Bst2UI CCWGG 1 cut(s) 821
Bst4CI ACNGT 4 cut(s) 335, 389, 586, 1037
Bst6I CTCTTC 1 cut(s) 848
BstAFI CTTAAG 1 cut(s) 731
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 1 cut(s) 472
BstF5I GGATG 1 cut(s) 1006
BstKTI GATC 2 cut(s) 961, 980
BstMBI GATC 2 cut(s) 958, 977
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 1 cut(s) 676
BstNI CCWGG 1 cut(s) 821
BstSCI CCNGG 1 cut(s) 819
BstSLI GKGCMC 1 cut(s) 275
BstV1I GCAGC 2 cut(s) 644, 829
BsuRI GGCC 1 cut(s) 70
BtgZI GCGATG 1 cut(s) 438
BtsCI GGATG 1 cut(s) 1006
BtsI GCAGTG 2 cut(s) 412, 751
BtsIMutI CAGTG 9 cut(s) 340, 388, 394, 412, 582, 751, 845, 977, 1099
Cac8I GCNNGC 1 cut(s) 403
CciI TCATGA 1 cut(s) 1012
Cfr10I RCCGGY 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 69
CseI GACGC 1 cut(s) 930
Csp6I GTAC 1 cut(s) 111
CviAII CATG 4 cut(s) 593, 698, 994, 1013
CviJI RGCY 7 cut(s) 70, 101, 254, 395, 728, 1081, 1102
CviKI_1 RGCY 7 cut(s) 70, 101, 254, 395, 728, 1081, 1102
CviQI GTAC 1 cut(s) 111
DdeI CTNAG 1 cut(s) 472
DpnI GATC 2 cut(s) 960, 979
DpnII GATC 2 cut(s) 958, 977
DrdI GACNNNNNNGTC 1 cut(s) 1040
DseDI GACNNNNNNGTC 1 cut(s) 1040
Eam1104I CTCTTC 1 cut(s) 848
EarI CTCTTC 1 cut(s) 848
Eco130I CCWWGG 2 cut(s) 671, 915
Eco32I GATATC 2 cut(s) 367, 826
Eco57I CTGAAG 2 cut(s) 270, 869
EcoRII CCWGG 1 cut(s) 819
EcoRV GATATC 2 cut(s) 367, 826
EcoT14I CCWWGG 2 cut(s) 671, 915
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 2 cut(s) 671, 915
FaeI CATG 4 cut(s) 596, 701, 997, 1016
FaqI GGGAC 2 cut(s) 933, 1076
FatI CATG 4 cut(s) 592, 697, 993, 1012
Fnu4HI GCNGC 2 cut(s) 633, 843
FokI GGATG 1 cut(s) 1013
Fsp4HI GCNGC 2 cut(s) 633, 843
FspBI CTAG 2 cut(s) 402, 672
GluI GCNGC 2 cut(s) 633, 843
HaeIII GGCC 1 cut(s) 70
HapII CCGG 2 cut(s) 67, 231
HgaI GACGC 1 cut(s) 930
Hin1II CATG 4 cut(s) 596, 701, 997, 1016
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 4 cut(s) 104, 281, 317, 338
HpaII CCGG 2 cut(s) 67, 231
HphI GGTGA 3 cut(s) 65, 285, 776
Hpy166II GTNNAC 2 cut(s) 152, 871
Hpy188I TCNGA 2 cut(s) 544, 1108
Hpy188III TCNNGA 5 cut(s) 39, 278, 474, 1013, 1031
Hpy8I GTNNAC 2 cut(s) 152, 871
HpyAV CCTTC 2 cut(s) 566, 1113
HpyCH4III ACNGT 4 cut(s) 335, 389, 586, 1037
HpyCH4IV ACGT 1 cut(s) 873
HpyCH4V TGCA 7 cut(s) 128, 173, 428, 483, 723, 845, 1071
HpyF10VI GCNNNNNNNGC 1 cut(s) 676
HpyF3I CTNAG 1 cut(s) 472
HpySE526I ACGT 1 cut(s) 873
Hsp92II CATG 4 cut(s) 596, 701, 997, 1016
Kzo9I GATC 2 cut(s) 958, 977
LmnI GCTCC 1 cut(s) 392
LpnPI CCDG 9 cut(s) 29, 80, 244, 261, 394, 459, 806, 833, 1105
Lsp1109I GCAGC 2 cut(s) 644, 829
LweI GCATC 2 cut(s) 415, 710
MaeI CTAG 2 cut(s) 402, 672
MaeII ACGT 1 cut(s) 873
MaeIII GTNAC 7 cut(s) 242, 305, 343, 383, 580, 1042, 1087
MalI GATC 2 cut(s) 960, 979
MboI GATC 2 cut(s) 958, 977
MboII GAAGA 4 cut(s) 252, 862, 865, 1019
MhlI GDGCHC 1 cut(s) 275
MluCI AATT 6 cut(s) 13, 459, 546, 701, 837, 1054
MmeI TCCRAC 1 cut(s) 693
MnlI CCTC 7 cut(s) 190, 331, 454, 573, 821, 991, 1053
Mph1103I ATGCAT 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 1102
MseI TTAA 4 cut(s) 549, 732, 803, 990
MslI CAYNNNNRTG 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 101
MspCI CTTAAG 1 cut(s) 731
MspI CCGG 2 cut(s) 67, 231
MspR9I CCNGG 1 cut(s) 821
MvaI CCWGG 1 cut(s) 821
MwoI GCNNNNNNNGC 1 cut(s) 676
NdeII GATC 2 cut(s) 958, 977
NheI GCTAGC 1 cut(s) 401
NlaIII CATG 4 cut(s) 596, 701, 997, 1016
NmuCI GTSAC 4 cut(s) 305, 383, 580, 1042
NsiI ATGCAT 1 cut(s) 430
PagI TCATGA 1 cut(s) 1012
PdmI GAANNNNTTC 1 cut(s) 1102
PfeI GAWTC 4 cut(s) 104, 281, 317, 338
PflFI GACNNNGTC 1 cut(s) 355
PkrI GCNGC 2 cut(s) 634, 844
PshBI ATTAAT 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 819
PspGI CCWGG 1 cut(s) 819
PspPI GGNCC 1 cut(s) 69
PsyI GACNNNGTC 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 4 cut(s) 549, 732, 803, 990
SatI GCNGC 2 cut(s) 633, 843
Sau3AI GATC 2 cut(s) 958, 977
Sau96I GGNCC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 821
SduI GDGCHC 1 cut(s) 275
SfaNI GCATC 2 cut(s) 415, 710
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 3 cut(s) 276, 731, 964
SmoI CTYRAG 3 cut(s) 276, 731, 964
Sse9I AATT 6 cut(s) 13, 459, 546, 701, 837, 1054
SspMI CTAG 2 cut(s) 402, 672
StyD4I CCNGG 1 cut(s) 819
StyI CCWWGG 2 cut(s) 671, 915
TaaI ACNGT 4 cut(s) 335, 389, 586, 1037
TaiI ACGT 1 cut(s) 876
TaqI TCGA 6 cut(s) 17, 89, 323, 662, 689, 835
TaqII GACCGA 1 cut(s) 35
TasI AATT 6 cut(s) 13, 459, 546, 701, 837, 1054
TfiI GAWTC 4 cut(s) 104, 281, 317, 338
Tru1I TTAA 4 cut(s) 549, 732, 803, 990
Tru9I TTAA 4 cut(s) 549, 732, 803, 990
TscAI CASTG 9 cut(s) 340, 388, 394, 412, 589, 751, 852, 977, 1099
TseFI GTSAC 4 cut(s) 305, 383, 580, 1042
TseI GCWGC 2 cut(s) 632, 842
Tsp45I GTSAC 4 cut(s) 305, 383, 580, 1042
TspDTI ATGAA 5 cut(s) 581, 585, 714, 1001, 1029
TspRI CASTG 9 cut(s) 340, 388, 394, 412, 589, 751, 852, 977, 1099
Tth111I GACNNNGTC 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 731
VspI ATTAAT 1 cut(s) 549
XapI RAATTY 2 cut(s) 701, 1054
XmaJI CCTAGG 1 cut(s) 671
XmnI GAANNNNTTC 1 cut(s) 1102
XspI CTAG 2 cut(s) 402, 672
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.