MD01G1028100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014549
Physical Location & Seq
Forward (+)
10457475 .. 10458705
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1028100.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGCTCCACTCATGTACTTGAGTTTCGGAAAGGTACTCCTGCTTTCCTCTGCTGTTTTCTTCCTCTTTCCTACTCTCCTCCTTTCTGCCCGCTCTGAGTCAGTTGAGACCTCACGGTTTGTTGCGAGTACAAGGAAGATCTTGCTAGAATTAGAAATCGACCCAGGCCAAGACCTGCCCAAAAAGATCAAGTCCACCACCACCAACCAATCCTCAAAAAACCAGACCAAATTGATCAAACCAACCTCGTCATCCTCAAAAAACCAGACCAAACTCCTCAAACCCAGTCTATCCTCCTCCAAGAACCAAACCAGACTCCTCGAACCCAATCTATCCTCCCCCAAGAACCAAACCAAGATTTTGAAATCCACCAAGTCCAACTCCACCAAGAATGGCGAGTTGAAGAAGCTCAATCCCACATCAAAACCCTCAAATTCCACCACACCCACATCAAATTCCTCGAAGAAAACCTCAGATCTAACTAAAATAAGCTTACCCAAGAACCAAACCACCAAACCCACCACCCCGAAACAATCCCAAAACCTGGTCGACAAGAAAAAAACCACCGACGCTAAAATACCAGCCCAACAAAAACCCAAAAAACCAGTCGAACCCAGCTGGATCGACCAAGAAGACGACACCGATTTCGTCTCCGACTTCACCGACTTACCCGGCAAATTCCAGCAAACCCTAATCCCAGATTTGGAGAGAATTCGGACCACCTCGAAGATTTACCTCACCAAAGCCAACAAACAAATGACGAACCAGTTCAAGCCCATTGTCGGTAAGAAGTACGCCGCCACCATCGCCTCCACGGTGTCGTGCGTCTTCCTGATTATCCCTCTGCTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

31.42

Weight (kDa)

10.06

Isoelectric Point (pI)

29.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39840
malus_domestica MD01G1028100.v1.1 MD15G1325300.v1.1
prunus_persica Prupe.6G184700_v2.0.a1
pyrus_communis pycom01g05640
rosa_chinensis RchiOBHm_Chr2g0114541
rosa_laevigata RLG00000018113
rosa_multiflora Rmu_sc0000370.1_g000001 Rmu_sc0000371.1_g000011 Rmu_sc0027184.1_g000005
rosa_roxburghii Rroxscaffold_2G00130280
rosa_rugosa Rorug02G0190700
rosa_samantha Rh2AG248600 Rh2BG258200 Rh2CG252000 Rh2DG256700
rosa_wichuraiana Rw2G019330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 544
AccBSI CCGCTC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 549
AciI CCGC 2 cut(s) 91, 798
AclWI GGATC 1 cut(s) 629
AcsI RAATTY 4 cut(s) 433, 454, 677, 711
AfaI GTAC 4 cut(s) 17, 36, 130, 794
AfiI CCNNNNNNNGG 3 cut(s) 544, 703, 782
AgsI TTSAA 3 cut(s) 364, 403, 772
AjnI CCWGG 2 cut(s) 163, 543
AluBI AGCT 3 cut(s) 409, 492, 618
AluI AGCT 3 cut(s) 409, 492, 618
Alw26I GTCTC 2 cut(s) 101, 655
AlwI GGATC 1 cut(s) 629
AoxI GGCC 1 cut(s) 166
ApoI RAATTY 4 cut(s) 433, 454, 677, 711
ArsI GACNNNNNNTTYG 4 cut(s) 591, 623, 629, 661
Asp700I GAANNNNTTC 1 cut(s) 767
AspS9I GGNCC 1 cut(s) 717
AsuC2I CCSGG 1 cut(s) 672
AsuHPI GGTGA 2 cut(s) 652, 730
AvaII GGWCC 1 cut(s) 717
BbsI GAAGAC 2 cut(s) 639, 820
BccI CCATC 1 cut(s) 812
BciT130I CCWGG 2 cut(s) 165, 545
BclI TGATCA 1 cut(s) 234
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 2 cut(s) 101, 655
BfaI CTAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 183
BglII AGATCT 2 cut(s) 138, 475
BisI GCNGC 1 cut(s) 798
BlsI GCNGC 1 cut(s) 799
Bme1390I CCNGG 3 cut(s) 165, 545, 672
Bme18I GGWCC 1 cut(s) 717
BmgT120I GGNCC 1 cut(s) 717
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 165, 545, 672
BmrI ACTGGG 1 cut(s) 279
BmuI ACTGGG 1 cut(s) 279
BpiI GAAGAC 2 cut(s) 639, 820
BpuEI CTTGAG 1 cut(s) 40
BpuMI CCSGG 1 cut(s) 672
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 163, 813
Bsc4I CCNNNNNNNGG 3 cut(s) 544, 703, 782
Bse1I ACTGG 3 cut(s) 285, 605, 766
BseBI CCWGG 2 cut(s) 165, 545
BseDI CCNNGG 2 cut(s) 163, 813
BseGI GGATG 1 cut(s) 251
BseLI CCNNNNNNNGG 3 cut(s) 544, 703, 782
BseMII CTCAG 2 cut(s) 87, 486
BseNI ACTGG 3 cut(s) 285, 605, 766
BseRI GAGGAG 4 cut(s) 68, 266, 286, 308
BseYI CCCAGC 1 cut(s) 614
BshFI GGCC 1 cut(s) 168
BsiSI CCGG 1 cut(s) 672
BslI CCNNNNNNNGG 3 cut(s) 544, 703, 782
BsmAI GTCTC 2 cut(s) 101, 655
BsmBI CGTCTC 1 cut(s) 655
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 1 cut(s) 101
Bsp143I GATC 5 cut(s) 138, 186, 234, 475, 621
BspACI CCGC 2 cut(s) 91, 798
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 2 cut(s) 88, 485
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 183
BspPI GGATC 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 93
BsrI ACTGG 3 cut(s) 285, 605, 766
BssECI CCNNGG 2 cut(s) 163, 813
BssMI GATC 5 cut(s) 138, 186, 234, 475, 621
Bst2UI CCWGG 2 cut(s) 165, 545
Bst4CI ACNGT 2 cut(s) 117, 817
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 2 cut(s) 96, 472
BstDSI CCRYGG 1 cut(s) 813
BstF5I GGATG 1 cut(s) 251
BstKTI GATC 5 cut(s) 141, 189, 237, 478, 624
BstMAI GTCTC 2 cut(s) 101, 655
BstMBI GATC 5 cut(s) 138, 186, 234, 475, 621
BstMWI GCNNNNNNNGC 1 cut(s) 806
BstNI CCWGG 2 cut(s) 165, 545
BstSCI CCNGG 3 cut(s) 163, 543, 670
BstV2I GAAGAC 2 cut(s) 639, 820
BstX2I RGATCY 2 cut(s) 138, 475
BstYI RGATCY 2 cut(s) 138, 475
BsuRI GGCC 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 813
BtgZI GCGATG 1 cut(s) 790
BtsCI GGATG 1 cut(s) 251
BveI ACCTGC 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 717
CseI GACGC 2 cut(s) 578, 814
CsiI ACCWGGT 1 cut(s) 543
Csp6I GTAC 4 cut(s) 16, 35, 129, 793
CviAII CATG 1 cut(s) 13
CviJI RGCY 8 cut(s) 5, 168, 409, 492, 584, 618, 746, 775
CviKI_1 RGCY 8 cut(s) 5, 168, 409, 492, 584, 618, 746, 775
CviQI GTAC 4 cut(s) 16, 35, 129, 793
DdeI CTNAG 2 cut(s) 96, 472
DpnI GATC 5 cut(s) 140, 188, 236, 477, 623
DpnII GATC 5 cut(s) 138, 186, 234, 475, 621
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 717
EcoRI GAATTC 1 cut(s) 711
EcoRII CCWGG 2 cut(s) 163, 543
Esp3I CGTCTC 1 cut(s) 655
FaeI CATG 1 cut(s) 16
FaiI YATR 1 cut(s) 14
FatI CATG 1 cut(s) 12
FauI CCCGC 1 cut(s) 98
FbaI TGATCA 1 cut(s) 234
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 1 cut(s) 798
FokI GGATG 1 cut(s) 238
Fsp4HI GCNGC 1 cut(s) 798
FspBI CTAG 1 cut(s) 146
GluI GCNGC 1 cut(s) 798
GsaI CCCAGC 1 cut(s) 618
HaeIII GGCC 1 cut(s) 168
HapII CCGG 1 cut(s) 672
HgaI GACGC 2 cut(s) 578, 814
Hin1II CATG 1 cut(s) 16
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 490
HinfI GANTC 2 cut(s) 98, 315
HpaII CCGG 1 cut(s) 672
HphI GGTGA 2 cut(s) 652, 730
Hpy166II GTNNAC 2 cut(s) 195, 550
Hpy188I TCNGA 5 cut(s) 29, 97, 475, 655, 717
Hpy188III TCNNGA 2 cut(s) 832, 852
Hpy8I GTNNAC 2 cut(s) 195, 550
Hpy99I CGWCG 1 cut(s) 572
HpyCH4III ACNGT 2 cut(s) 117, 817
HpyF10VI GCNNNNNNNGC 1 cut(s) 806
HpyF3I CTNAG 2 cut(s) 96, 472
Hsp92II CATG 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 5 cut(s) 138, 186, 234, 475, 621
LmnI GCTCC 1 cut(s) 10
MabI ACCWGGT 1 cut(s) 543
MaeI CTAG 1 cut(s) 146
MalI GATC 5 cut(s) 140, 188, 236, 477, 623
MbiI CCGCTC 1 cut(s) 93
MboI GATC 5 cut(s) 138, 186, 234, 475, 621
MboII GAAGA 7 cut(s) 52, 148, 415, 475, 644, 739, 820
MflI RGATCY 2 cut(s) 138, 475
MluCI AATT 6 cut(s) 149, 230, 433, 454, 677, 711
MlyI GAGTC 2 cut(s) 107, 309
MmeI TCCRAC 2 cut(s) 402, 678
MroXI GAANNNNTTC 1 cut(s) 767
MspA1I CMGCKG 1 cut(s) 618
MspI CCGG 1 cut(s) 672
MspR9I CCNGG 3 cut(s) 165, 545, 672
MvaI CCWGG 2 cut(s) 165, 545
MwoI GCNNNNNNNGC 1 cut(s) 806
NciI CCSGG 1 cut(s) 672
NdeII GATC 5 cut(s) 138, 186, 234, 475, 621
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 767
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 1 cut(s) 799
PleI GAGTC 2 cut(s) 106, 309
PpsI GAGTC 2 cut(s) 106, 309
Psp6I CCWGG 2 cut(s) 163, 543
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 2 cut(s) 163, 543
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 717
PsuI RGATCY 2 cut(s) 138, 475
PvuII CAGCTG 1 cut(s) 618
RsaI GTAC 4 cut(s) 17, 36, 130, 794
RsaNI GTAC 4 cut(s) 16, 35, 129, 793
SalI GTCGAC 1 cut(s) 548
SatI GCNGC 1 cut(s) 798
Sau3AI GATC 5 cut(s) 138, 186, 234, 475, 621
Sau96I GGNCC 1 cut(s) 717
SchI GAGTC 2 cut(s) 107, 309
ScrFI CCNGG 3 cut(s) 165, 545, 672
SexAI ACCWGGT 1 cut(s) 543
SinI GGWCC 1 cut(s) 717
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
Sse9I AATT 6 cut(s) 149, 230, 433, 454, 677, 711
SsiI CCGC 2 cut(s) 91, 798
SspMI CTAG 1 cut(s) 146
StyD4I CCNGG 3 cut(s) 163, 543, 670
TaaI ACNGT 2 cut(s) 117, 817
TaqI TCGA 7 cut(s) 159, 321, 461, 549, 609, 624, 725
TasI AATT 6 cut(s) 149, 230, 433, 454, 677, 711
TatI WGTACW 2 cut(s) 15, 128
TauI GCSGC 1 cut(s) 800
Van91I CCANNNNNTGG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 4 cut(s) 433, 454, 677, 711
XmiI GTMKAC 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 767
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.