RLG00000018113

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014549
Physical Location & Seq
Reverse (-)
25431754 .. 25433103
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018113

Sequence Viewer

Length: 1350 bp
ATGGCTCCACTCATGTACTTCAAGTTCAGAAAGGTACTTTTGCTAGTTTCTCTTGTTGTGTTCCTCTTTTCTTCTACTCTGCTCCTTTCTGCCGCAGAAGAAGATGAGAAACTGCCTCGAATTCAGAGCTCATCACAGTTTTTGGCTTCAAGGAAAATGTTGGAATTAGATGAAGAAGATGAAGAAGTCCAGCCTAAGAAGATCAAGACCAAAACCACCACCTCAACTCCCCAATCCTCCAAAAACCAAACCAAGCTTATCAAACCCACTTCATCATCCTCCAAAAACCAAACCAAGCCGCTCAAGCCACTTAAACCCAACCTATCATCCAAGAACCAAACCAAGCTCATCAAACCAAGCACCAATTCAACCAAGTCATCATCATCAGCACCACCCAGTATCAAGATTGGCCTCAAGAAGCTCAATTCCACTTCCAAACCCTCAAACTCCACCAAACCCGCTTCACTATCCTCCAAGAAAACCACAGATCTAGCCAAACTAACCTCCCCCAAGAACAAAACAACCAAACCCACCACCACAAAACAACCTCAAACTCCCAAACCAATCCAAAAAACACAGAAAACCCCCACCCCACAAAAACCCGCCAAGAAACCAATCCAACCCAGCTGGATCGAACAAGAAGACGACACCGACTTCGTCTCCGACTTCACAGACTTACCCGGCCAATTCCAACAGACTCTGATCCCAGACCTGGAGAAAATCTCAACCACATCAAAAATCTACCTCACCCAAGCCAACAAGCAAATGACCACCAGGTTCAAACCACTCGTCGGAAACAAATACGCCCCCACCATCGCTTCCATAGTCTCCTGCGCATTCCTACTCATCCCATTGCTCCTAGTCTCTCTCCTCTTCAACCGCATCAAAGCCTACTTTTCCCTCCAAAAGCTCCTCATCTTCATCCAAGTCTACCTCTCCATCTACTTCTCAATCCTCTGCCTCTCTTCCTTGGTCACTGGCCTCGAGCCCCTCAAGTTCTTCTACGCTACTTCGCAGTCCACCTACATCTGCCTCCAGGTTCTGCAGACTCTCGGCTACGTGCTCTATCTCCTCCTCCTCATCATGTACTTGATCCTGGTTTTCTCGACCGACTCCGGGCTGGGCTCGAAGATGTTGGGACTGGCCCAGAGTTTTCTCGGGTTCGCGGTTGGGCTGCATTACTACATGTCGGTGTTTCACAGGGCGGTGCTGCGGCAGCCGCCGAAGACTAATTGGAAGGTGCACGGGATATACGCCATGTGTTACCTTGTGATTTGTCTGTTTGCCACGGCGGAGAGGAGGAAGAAGGCTTACTTGGAAGAAGGTGGTGAAGAGGGCAAGAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

450

Amino Acids

50.25

Weight (kDa)

9.83

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39840
malus_domestica MD01G1028100.v1.1 MD15G1325300.v1.1
prunus_persica Prupe.6G184700_v2.0.a1
pyrus_communis pycom01g05640
rosa_chinensis RchiOBHm_Chr2g0114541
rosa_laevigata RLG00000018113
rosa_multiflora Rmu_sc0000370.1_g000001 Rmu_sc0000371.1_g000011 Rmu_sc0027184.1_g000005
rosa_roxburghii Rroxscaffold_2G00130280
rosa_rugosa Rorug02G0190700
rosa_samantha Rh2AG248600 Rh2BG258200 Rh2CG252000 Rh2DG256700
rosa_wichuraiana Rw2G019330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 835
AccBSI CCGCTC 1 cut(s) 301
AccI GTMKAC 1 cut(s) 930
AccII CGCG 1 cut(s) 1166
AclWI GGATC 3 cut(s) 638, 697, 1087
AcoI YGGCCR 1 cut(s) 682
AcsI RAATTY 1 cut(s) 120
AfaI GTAC 3 cut(s) 17, 36, 1088
AfiI CCNNNNNNNGG 3 cut(s) 712, 791, 1116
AflIII ACRYGT 1 cut(s) 1185
AgsI TTSAA 5 cut(s) 22, 150, 369, 781, 877
AjnI CCWGG 4 cut(s) 711, 773, 1035, 1095
AjuI GAANNNNNNNTTGG 4 cut(s) 349, 381, 1298, 1330
AluBI AGCT 6 cut(s) 129, 256, 346, 421, 627, 910
AluI AGCT 6 cut(s) 129, 256, 346, 421, 627, 910
Alw21I GWGCWC 3 cut(s) 131, 1065, 1245
Alw26I GTCTC 3 cut(s) 664, 832, 868
Alw44I GTGCAC 1 cut(s) 1241
AlwI GGATC 3 cut(s) 638, 697, 1087
AlwNI CAGNNNCTG 2 cut(s) 700, 1042
Ama87I CYCGRG 2 cut(s) 983, 1157
AoxI GGCC 4 cut(s) 409, 682, 979, 1143
ApaLI GTGCAC 1 cut(s) 1241
ApeKI GCWGC 3 cut(s) 1174, 1210, 1216
ApoI RAATTY 1 cut(s) 120
ArsI GACNNNNNNTTYG 4 cut(s) 638, 670, 774, 806
AspLEI GCGC 1 cut(s) 836
AspS9I GGNCC 1 cut(s) 1144
AsuC2I CCSGG 2 cut(s) 681, 1117
AsuHPI GGTGA 2 cut(s) 739, 1340
AvaI CYCGRG 2 cut(s) 983, 1157
BaeGI GKGCMC 1 cut(s) 1245
BanII GRGCYC 3 cut(s) 131, 990, 1127
BarI GAAGNNNNNNTAC 2 cut(s) 1295, 1327
BbsI GAAGAC 2 cut(s) 648, 1232
Bbv12I GWGCWC 3 cut(s) 131, 1065, 1245
BbvI GCAGC 3 cut(s) 1161, 1197, 1228
BccI CCATC 2 cut(s) 821, 947
BceAI ACGGC 1 cut(s) 1305
BciT130I CCWGG 4 cut(s) 713, 775, 1037, 1097
BcnI CCSGG 2 cut(s) 681, 1117
BcoDI GTCTC 3 cut(s) 664, 832, 868
BfaI CTAG 3 cut(s) 44, 491, 860
BfmI CTRYAG 1 cut(s) 1043
BglII AGATCT 1 cut(s) 487
BisI GCNGC 7 cut(s) 93, 299, 1175, 1211, 1214, 1217, 1220
BlsI GCNGC 7 cut(s) 94, 300, 1176, 1212, 1215, 1218, 1221
Bme1390I CCNGG 6 cut(s) 681, 713, 775, 1037, 1097, 1117
BmeT110I CYCGRG 2 cut(s) 983, 1157
BmgT120I GGNCC 1 cut(s) 1144
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 6 cut(s) 681, 713, 775, 1037, 1097, 1117
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 1 cut(s) 891
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 2 cut(s) 648, 1232
BplI GAGNNNNNCTC 2 cut(s) 707, 739
BpmI CTGGAG 2 cut(s) 734, 1019
BpuEI CTTGAG 3 cut(s) 287, 398, 977
BpuMI CCSGG 2 cut(s) 681, 1117
BsaAI YACGTR 1 cut(s) 1060
BsaJI CCNNGG 2 cut(s) 969, 1287
BsaXI ACNNNNNCTCC 4 cut(s) 211, 241, 644, 674
Bsc4I CCNNNNNNNGG 3 cut(s) 712, 791, 1116
Bse1I ACTGG 3 cut(s) 396, 982, 1146
Bse3DI GCAATG 1 cut(s) 851
BseBI CCWGG 4 cut(s) 713, 775, 1037, 1097
BseDI CCNNGG 2 cut(s) 969, 1287
BseGI GGATG 4 cut(s) 275, 326, 846, 921
BseLI CCNNNNNNNGG 3 cut(s) 712, 791, 1116
BseMI GCAATG 1 cut(s) 851
BseNI ACTGG 3 cut(s) 396, 982, 1146
BseRI GAGGAG 6 cut(s) 860, 902, 1061, 1064, 1067, 1312
BseSI GKGCMC 1 cut(s) 1245
BseXI GCAGC 3 cut(s) 1161, 1197, 1228
BseYI CCCAGC 2 cut(s) 623, 1120
Bsh1236I CGCG 1 cut(s) 1166
Bsh1285I CGRYCG 1 cut(s) 1110
BshFI GGCC 4 cut(s) 411, 684, 981, 1145
BsiEI CGRYCG 1 cut(s) 1110
BsiHKAI GWGCWC 3 cut(s) 131, 1065, 1245
BsiHKCI CYCGRG 2 cut(s) 983, 1157
BsiSI CCGG 2 cut(s) 681, 1116
BslFI GGGAC 1 cut(s) 1152
BslI CCNNNNNNNGG 3 cut(s) 712, 791, 1116
BsmAI GTCTC 3 cut(s) 664, 832, 868
BsmBI CGTCTC 1 cut(s) 664
BsmFI GGGAC 1 cut(s) 1152
BsmI GAATGC 1 cut(s) 836
BsnI GGCC 4 cut(s) 411, 684, 981, 1145
BsoBI CYCGRG 2 cut(s) 983, 1157
Bsp1286I GDGCHC 5 cut(s) 131, 990, 1065, 1127, 1245
Bsp143I GATC 5 cut(s) 201, 487, 630, 702, 1092
BspANI GGCC 4 cut(s) 411, 684, 981, 1145
BspFNI CGCG 1 cut(s) 1166
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 1047
BspPI GGATC 3 cut(s) 638, 697, 1087
BsrBI CCGCTC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 851
BsrI ACTGG 3 cut(s) 396, 982, 1146
BssECI CCNNGG 2 cut(s) 969, 1287
BssMI GATC 5 cut(s) 201, 487, 630, 702, 1092
BssT1I CCWWGG 1 cut(s) 969
Bst2UI CCWGG 4 cut(s) 713, 775, 1037, 1097
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 3 cut(s) 878, 970, 1326
BstBAI YACGTR 1 cut(s) 1060
BstDEI CTNAG 1 cut(s) 195
BstDSI CCRYGG 1 cut(s) 1287
BstF5I GGATG 4 cut(s) 275, 326, 846, 921
BstFNI CGCG 1 cut(s) 1166
BstHHI GCGC 1 cut(s) 836
BstKTI GATC 5 cut(s) 204, 490, 633, 705, 1095
BstMAI GTCTC 3 cut(s) 664, 832, 868
BstMBI GATC 5 cut(s) 201, 487, 630, 702, 1092
BstMCI CGRYCG 1 cut(s) 1110
BstMWI GCNNNNNNNGC 3 cut(s) 304, 1216, 1219
BstNI CCWGG 4 cut(s) 713, 775, 1037, 1097
BstNSI RCATGY 1 cut(s) 1189
BstSCI CCNGG 6 cut(s) 679, 711, 773, 1035, 1095, 1115
BstSFI CTRYAG 1 cut(s) 1043
BstSLI GKGCMC 1 cut(s) 1245
BstUI CGCG 1 cut(s) 1166
BstV1I GCAGC 3 cut(s) 1161, 1197, 1228
BstV2I GAAGAC 2 cut(s) 648, 1232
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BsuRI GGCC 4 cut(s) 411, 684, 981, 1145
BtgI CCRYGG 1 cut(s) 1287
BtgZI GCGATG 1 cut(s) 799
BtsCI GGATG 4 cut(s) 275, 326, 846, 921
BtsIMutI CAGTG 1 cut(s) 975
CaiI CAGNNNCTG 2 cut(s) 700, 1042
CfoI GCGC 1 cut(s) 836
Cfr13I GGNCC 1 cut(s) 1144
CsiI ACCWGGT 1 cut(s) 773
Csp6I GTAC 3 cut(s) 16, 35, 1087
CviAII CATG 4 cut(s) 13, 1084, 1186, 1258
CviQI GTAC 3 cut(s) 16, 35, 1087
DdeI CTNAG 1 cut(s) 195
DpnI GATC 5 cut(s) 203, 489, 632, 704, 1094
DpnII GATC 5 cut(s) 201, 487, 630, 702, 1092
EaeI YGGCCR 1 cut(s) 682
Eam1104I CTCTTC 3 cut(s) 878, 970, 1326
EarI CTCTTC 3 cut(s) 878, 970, 1326
EciI GGCGGA 1 cut(s) 1307
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 969
Eco24I GRGCYC 3 cut(s) 131, 990, 1127
Eco53kI GAGCTC 1 cut(s) 129
Eco88I CYCGRG 2 cut(s) 983, 1157
EcoICRI GAGCTC 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 4 cut(s) 711, 773, 1035, 1095
EcoT14I CCWWGG 1 cut(s) 969
EcoT38I GRGCYC 3 cut(s) 131, 990, 1127
ErhI CCWWGG 1 cut(s) 969
Esp3I CGTCTC 1 cut(s) 664
FaeI CATG 4 cut(s) 16, 1087, 1189, 1261
FaiI YATR 6 cut(s) 14, 824, 1085, 1187, 1252, 1259
FalI AAGNNNNNCTT 2 cut(s) 1298, 1330
FaqI GGGAC 1 cut(s) 1152
FatI CATG 4 cut(s) 12, 1083, 1185, 1257
FauI CCCGC 2 cut(s) 466, 610
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 7 cut(s) 93, 299, 1175, 1211, 1214, 1217, 1220
FokI GGATG 4 cut(s) 262, 313, 833, 908
FriOI GRGCYC 3 cut(s) 131, 990, 1127
Fsp4HI GCNGC 7 cut(s) 93, 299, 1175, 1211, 1214, 1217, 1220
FspBI CTAG 3 cut(s) 44, 491, 860
FspI TGCGCA 1 cut(s) 835
GlaI GCGC 1 cut(s) 835
GluI GCNGC 7 cut(s) 93, 299, 1175, 1211, 1214, 1217, 1220
GsaI CCCAGC 2 cut(s) 627, 1124
GsuI CTGGAG 2 cut(s) 734, 1019
HaeIII GGCC 4 cut(s) 411, 684, 981, 1145
HapII CCGG 2 cut(s) 681, 1116
HhaI GCGC 1 cut(s) 836
Hin1II CATG 4 cut(s) 16, 1087, 1189, 1261
Hin6I GCGC 1 cut(s) 834
HinP1I GCGC 1 cut(s) 834
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 3 cut(s) 697, 1048, 1112
HpaII CCGG 2 cut(s) 681, 1116
HphI GGTGA 2 cut(s) 739, 1340
Hpy166II GTNNAC 3 cut(s) 931, 1020, 1243
Hpy188I TCNGA 5 cut(s) 29, 126, 664, 702, 794
Hpy188III TCNNGA 4 cut(s) 205, 403, 415, 1105
Hpy8I GTNNAC 3 cut(s) 931, 1020, 1243
Hpy99I CGWCG 1 cut(s) 794
HpyAV CCTTC 3 cut(s) 1231, 1300, 1316
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4IV ACGT 1 cut(s) 1059
HpyCH4V TGCA 3 cut(s) 1045, 1177, 1243
HpyF10VI GCNNNNNNNGC 3 cut(s) 304, 1216, 1219
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 1059
Hsp92II CATG 4 cut(s) 16, 1087, 1189, 1261
HspAI GCGC 1 cut(s) 834
Kzo9I GATC 5 cut(s) 201, 487, 630, 702, 1092
LmnI GCTCC 4 cut(s) 10, 87, 861, 915
Lsp1109I GCAGC 3 cut(s) 1161, 1197, 1228
LweI GCATC 1 cut(s) 891
MabI ACCWGGT 1 cut(s) 773
MaeI CTAG 3 cut(s) 44, 491, 860
MaeII ACGT 1 cut(s) 1059
MaeIII GTNAC 2 cut(s) 973, 1262
MalI GATC 5 cut(s) 203, 489, 632, 704, 1094
MbiI CCGCTC 1 cut(s) 301
MboI GATC 5 cut(s) 201, 487, 630, 702, 1092
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 5 cut(s) 131, 990, 1065, 1127, 1245
MluCI AATT 6 cut(s) 120, 164, 364, 424, 686, 1231
MlyI GAGTC 3 cut(s) 691, 1042, 1106
MmeI TCCRAC 5 cut(s) 141, 643, 687, 715, 772
MseI TTAA 1 cut(s) 312
MslI CAYNNNNRTG 1 cut(s) 1190
MspA1I CMGCKG 1 cut(s) 627
MspI CCGG 2 cut(s) 681, 1116
MspR9I CCNGG 6 cut(s) 681, 713, 775, 1037, 1097, 1117
Mva1269I GAATGC 1 cut(s) 836
MvaI CCWGG 4 cut(s) 713, 775, 1037, 1097
MvnI CGCG 1 cut(s) 1166
MwoI GCNNNNNNNGC 3 cut(s) 304, 1216, 1219
NciI CCSGG 2 cut(s) 681, 1117
NdeII GATC 5 cut(s) 201, 487, 630, 702, 1092
NlaIII CATG 4 cut(s) 16, 1087, 1189, 1261
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 1032
NmuCI GTSAC 1 cut(s) 973
NsbI TGCGCA 1 cut(s) 835
NspI RCATGY 1 cut(s) 1189
PaeR7I CTCGAG 1 cut(s) 983
PciI ACATGT 1 cut(s) 1185
PctI GAATGC 1 cut(s) 836
PflFI GACNNNGTC 1 cut(s) 656
PkrI GCNGC 7 cut(s) 94, 300, 1176, 1212, 1215, 1218, 1221
PleI GAGTC 3 cut(s) 691, 1042, 1106
PpsI GAGTC 3 cut(s) 691, 1042, 1106
Ppu21I YACGTR 1 cut(s) 1060
PscI ACATGT 1 cut(s) 1185
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 4 cut(s) 711, 773, 1035, 1095
PspFI CCCAGC 2 cut(s) 623, 1120
PspGI CCWGG 4 cut(s) 711, 773, 1035, 1095
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 1144
PspXI VCTCGAGB 1 cut(s) 983
PsrI GAACNNNNNNTAC 2 cut(s) 8, 40
PstI CTGCAG 1 cut(s) 1047
PstNI CAGNNNCTG 2 cut(s) 700, 1042
PsuI RGATCY 1 cut(s) 487
PsyI GACNNNGTC 1 cut(s) 656
PvuII CAGCTG 1 cut(s) 627
RsaI GTAC 3 cut(s) 17, 36, 1088
RsaNI GTAC 3 cut(s) 16, 35, 1087
RseI CAYNNNNRTG 1 cut(s) 1190
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 7 cut(s) 93, 299, 1175, 1211, 1214, 1217, 1220
Sau3AI GATC 5 cut(s) 201, 487, 630, 702, 1092
Sau96I GGNCC 1 cut(s) 1144
SchI GAGTC 3 cut(s) 691, 1042, 1106
ScrFI CCNGG 6 cut(s) 681, 713, 775, 1037, 1097, 1117
SduI GDGCHC 5 cut(s) 131, 990, 1065, 1127, 1245
SexAI ACCWGGT 1 cut(s) 773
SfaNI GCATC 1 cut(s) 891
SfcI CTRYAG 1 cut(s) 1043
Sfr274I CTCGAG 1 cut(s) 983
SlaI CTCGAG 1 cut(s) 983
SmiMI CAYNNNNRTG 1 cut(s) 1190
SmlI CTYRAG 4 cut(s) 302, 413, 983, 992
SmoI CTYRAG 4 cut(s) 302, 413, 983, 992
Sse9I AATT 6 cut(s) 120, 164, 364, 424, 686, 1231
SspMI CTAG 3 cut(s) 44, 491, 860
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 6 cut(s) 679, 711, 773, 1035, 1095, 1115
StyI CCWWGG 1 cut(s) 969
TaaI ACNGT 1 cut(s) 138
TaiI ACGT 1 cut(s) 1062
TaqI TCGA 5 cut(s) 118, 633, 984, 1106, 1127
TaqII GACCGA 1 cut(s) 1124
TasI AATT 6 cut(s) 120, 164, 364, 424, 686, 1231
TatI WGTACW 2 cut(s) 15, 1086
TauI GCSGC 4 cut(s) 95, 301, 1216, 1222
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 1 cut(s) 982
TseFI GTSAC 1 cut(s) 973
TseI GCWGC 3 cut(s) 1174, 1210, 1216
Tsp45I GTSAC 1 cut(s) 973
TspDTI ATGAA 4 cut(s) 186, 195, 261, 910
TspRI CASTG 1 cut(s) 982
Tth111I GACNNNGTC 1 cut(s) 656
VneI GTGCAC 1 cut(s) 1241
XapI RAATTY 1 cut(s) 120
XceI RCATGY 1 cut(s) 1189
XhoI CTCGAG 1 cut(s) 983
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 3 cut(s) 44, 491, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.