MD15G1325300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014549
Physical Location & Seq
Reverse (-)
34784835 .. 34786205
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1325300.v1.1.491

Sequence Viewer

Length: 1371 bp
ATGGCTCCACTCATGTACTTGAGTTTCAGAAAGGTACTCCTGCTTTCCTCTGCTCTTGTCTTCCTCTTTCCTACTCTCCTCCTTTCTGCCCACTCTGAGTCCGTTGAGAGCTCACACTTTGTTGCCAGTACAAGGAAAATCTTGTTAGAATTAGAAATCGACCCCGACCAGGACCTGCCCAAAAAGACCAAGTCCTCCACCACCACCACCACCACCACCACCAAATCCTCAAAAAACCAGACCAAATTGATCAAACCCACCTTGTCTTCCTCAAAAAACAAGACCAAATTGATCAAACCAACCTTGTCTTCCTCGAAAAACCAGACCAAACTCCTCAAACCCAATCTGTCTTCCTCCAAGAACCAAACCAAGATTTTGAAATCCACCACCCATTCCAATTCCAATTCCAATTCCACCAAGAATGTCCAGTTGAAGAAGCTCAATCCCACATCAAAACCCTCAGATTCCACCAAACCCACATCAAATTCCTCGAAGAAAACCTCAGATTTCATCAAACCAAGCTCACCCAAGAACCAAACATCCAAACCCACCACCCCAAAACAATCCCACAACCTCGTCGACAAGAAAAAAACCACCGACACCAAAAGACCCACCCAACAAAAACCCAAAAAACCAATCGAACCCAGCTGGATCGACCAAGAAGACGACACCGATTTCGTGTCGGATTTCACCGGCTTACCCGGGAAATTCCAGCAATCCCTAATCCCAGATTTGGAGAGAATCTCGACCACCTCGAAGAATTACCTCACTAAAGCCAACAAACAAATGACGAACCAGTTCAAGCCCATTGTCGGCAACAAGTACGCCGCCACCATCGCCTCCGCCGTGTCGTGCGCCTTCCTGTTGATCCCTCTGCTGTTAGTCTCTCTCCTCTTCAACCGCATCAAAGCCTACTTCTCTCTCCAAAAGCTGTTGATCTTCGTCCAAGTCTACCTCTCCATCTACTTCTCCATACTCTGCCTCTCTGCTCTCGTCACCGGCCTCGAACCTCTCAAGTTTTTCTACGCTACTTCGCAGTCCACCTACGTCTGCCTGCAGGTACTGCAGACTCTCGGCTACGTCTTGTACCTGCTGCTGCTGCTCATGTACTTGGTTCTGGTTTTCTCAACCGATTCTGGGCTGGGCTCGAAAGTGTTGGGCCTGGCCCAGACATTCGTAGGGTTCGCCGTTGGGCTTCATTACTACATGTCGGTGTTTCATAGAGCTGTGCTCAAGCAGCCGCCCAAGACCAACTGGAAGGTTCATGGAATCTACGCCACGTGTTTTCTTGTGATTTGTCTGTTTGCCACCGCCGAGAGGAGGAAGAAGGGTTACTTGCAAGAGGGAGGTGAGGAGGGCAAGAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

457

Amino Acids

50.77

Weight (kDa)

9.9

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39840
malus_domestica MD01G1028100.v1.1 MD15G1325300.v1.1
prunus_persica Prupe.6G184700_v2.0.a1
pyrus_communis pycom01g05640
rosa_chinensis RchiOBHm_Chr2g0114541
rosa_laevigata RLG00000018113
rosa_multiflora Rmu_sc0000370.1_g000001 Rmu_sc0000371.1_g000011 Rmu_sc0027184.1_g000005
rosa_roxburghii Rroxscaffold_2G00130280
rosa_rugosa Rorug02G0190700
rosa_samantha Rh2AG248600 Rh2BG258200 Rh2CG252000 Rh2DG256700
rosa_wichuraiana Rw2G019330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 183, 1048, 1098
AccI GTMKAC 2 cut(s) 579, 951
AciI CCGC 5 cut(s) 828, 843, 901, 1241, 1311
AclWI GGATC 2 cut(s) 659, 862
AcsI RAATTY 2 cut(s) 484, 707
AcvI CACGTG 1 cut(s) 1281
AfaI GTAC 7 cut(s) 17, 36, 130, 824, 1062, 1088, 1109
AfiI CCNNNNNNNGG 7 cut(s) 132, 169, 733, 812, 1137, 1317, 1320
AflIII ACRYGT 2 cut(s) 1206, 1280
AgsI TTSAA 4 cut(s) 379, 433, 802, 898
AjnI CCWGG 2 cut(s) 168, 1161
AjuI GAANNNNNNNTTGG 2 cut(s) 292, 324
AloI GAACNNNNNNTCC 2 cut(s) 524, 556
AluBI AGCT 7 cut(s) 111, 439, 522, 648, 931, 1226, 1366
AluI AGCT 7 cut(s) 111, 439, 522, 648, 931, 1226, 1366
Alw21I GWGCWC 2 cut(s) 113, 1233
Alw26I GTCTC 1 cut(s) 889
AlwI GGATC 2 cut(s) 659, 862
AlwNI CAGNNNCTG 2 cut(s) 175, 1063
Ama87I CYCGRG 1 cut(s) 701
AoxI GGCC 3 cut(s) 1000, 1159, 1164
ApeKI GCWGC 4 cut(s) 1093, 1096, 1099, 1237
ApoI RAATTY 2 cut(s) 484, 707
ArsI GACNNNNNNTTYG 2 cut(s) 659, 691
Asp700I GAANNNNTTC 1 cut(s) 797
AspLEI GCGC 1 cut(s) 857
AspS9I GGNCC 3 cut(s) 172, 1159, 1165
AsuC2I CCSGG 2 cut(s) 702, 703
AsuHPI GGTGA 4 cut(s) 516, 682, 988, 1361
AvaI CYCGRG 1 cut(s) 701
AvaII GGWCC 1 cut(s) 172
BanII GRGCYC 2 cut(s) 113, 1148
BarI GAAGNNNNNNTAC 2 cut(s) 1316, 1348
BbrPI CACGTG 1 cut(s) 1281
BbsI GAAGAC 5 cut(s) 52, 258, 300, 342, 669
Bbv12I GWGCWC 2 cut(s) 113, 1233
BbvI GCAGC 4 cut(s) 1080, 1083, 1086, 1249
BccI CCATC 2 cut(s) 842, 968
BceAI ACGGC 2 cut(s) 830, 1172
BciT130I CCWGG 2 cut(s) 170, 1163
BclI TGATCA 2 cut(s) 249, 291
BcnI CCSGG 2 cut(s) 702, 703
BcoDI GTCTC 1 cut(s) 889
BfmI CTRYAG 2 cut(s) 1055, 1064
BfuAI ACCTGC 3 cut(s) 183, 1048, 1098
BisI GCNGC 6 cut(s) 828, 1094, 1097, 1100, 1238, 1241
BlsI GCNGC 6 cut(s) 829, 1095, 1098, 1101, 1239, 1242
Bme1390I CCNGG 4 cut(s) 170, 702, 703, 1163
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 701
BmgT120I GGNCC 3 cut(s) 172, 1159, 1165
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 4 cut(s) 170, 702, 703, 1163
BmsI GCATC 1 cut(s) 912
BpiI GAAGAC 5 cut(s) 52, 258, 300, 342, 669
BplI GAGNNNNNCTC 4 cut(s) 728, 760, 1215, 1247
BpuEI CTTGAG 3 cut(s) 40, 998, 1217
BpuMI CCSGG 2 cut(s) 702, 703
BsaAI YACGTR 1 cut(s) 1281
BsaJI CCNNGG 1 cut(s) 701
Bsc4I CCNNNNNNNGG 7 cut(s) 132, 169, 733, 812, 1137, 1317, 1320
Bse118I RCCGGY 2 cut(s) 692, 998
Bse1I ACTGG 4 cut(s) 126, 427, 796, 1259
BseBI CCWGG 2 cut(s) 170, 1163
BseDI CCNNGG 1 cut(s) 701
BseGI GGATG 1 cut(s) 539
BseLI CCNNNNNNNGG 7 cut(s) 132, 169, 733, 812, 1137, 1317, 1320
BseMII CTCAG 3 cut(s) 87, 474, 516
BseNI ACTGG 4 cut(s) 126, 427, 796, 1259
BseRI GAGGAG 5 cut(s) 68, 323, 881, 1333, 1367
BseXI GCAGC 4 cut(s) 1080, 1083, 1086, 1249
BseYI CCCAGC 2 cut(s) 644, 1141
BshFI GGCC 3 cut(s) 1002, 1161, 1166
BsiHKAI GWGCWC 2 cut(s) 113, 1233
BsiHKCI CYCGRG 1 cut(s) 701
BsiSI CCGG 3 cut(s) 693, 702, 999
BslI CCNNNNNNNGG 7 cut(s) 132, 169, 733, 812, 1137, 1317, 1320
BsmAI GTCTC 1 cut(s) 889
BsnI GGCC 3 cut(s) 1002, 1161, 1166
BsoBI CYCGRG 1 cut(s) 701
Bsp1286I GDGCHC 3 cut(s) 113, 1148, 1233
Bsp143I GATC 5 cut(s) 249, 291, 651, 867, 936
BspACI CCGC 5 cut(s) 828, 843, 901, 1241, 1311
BspANI GGCC 3 cut(s) 1002, 1161, 1166
BspCNI CTCAG 3 cut(s) 88, 473, 515
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 2 cut(s) 1059, 1068
BspMI ACCTGC 3 cut(s) 183, 1048, 1098
BspPI GGATC 2 cut(s) 659, 862
BsrFI RCCGGY 2 cut(s) 692, 998
BsrI ACTGG 4 cut(s) 126, 427, 796, 1259
BssAI RCCGGY 2 cut(s) 692, 998
BssECI CCNNGG 1 cut(s) 701
BssMI GATC 5 cut(s) 249, 291, 651, 867, 936
Bst2UI CCWGG 2 cut(s) 170, 1163
Bst6I CTCTTC 1 cut(s) 899
BstAPI GCANNNNNTGC 1 cut(s) 1063
BstBAI YACGTR 1 cut(s) 1281
BstC8I GCNNGC 1 cut(s) 1055
BstDEI CTNAG 3 cut(s) 96, 460, 502
BstF5I GGATG 1 cut(s) 539
BstHHI GCGC 1 cut(s) 857
BstKTI GATC 5 cut(s) 252, 294, 654, 870, 939
BstMAI GTCTC 1 cut(s) 889
BstMBI GATC 5 cut(s) 249, 291, 651, 867, 936
BstMWI GCNNNNNNNGC 4 cut(s) 836, 1063, 1099, 1237
BstNI CCWGG 2 cut(s) 170, 1163
BstNSI RCATGY 1 cut(s) 1210
BstSCI CCNGG 4 cut(s) 168, 700, 701, 1161
BstSFI CTRYAG 2 cut(s) 1055, 1064
BstV1I GCAGC 4 cut(s) 1080, 1083, 1086, 1249
BstV2I GAAGAC 5 cut(s) 52, 258, 300, 342, 669
BsuRI GGCC 3 cut(s) 1002, 1161, 1166
BtgZI GCGATG 1 cut(s) 820
BtsCI GGATG 1 cut(s) 539
BveI ACCTGC 3 cut(s) 183, 1048, 1098
Cac8I GCNNGC 1 cut(s) 1055
CaiI CAGNNNCTG 2 cut(s) 175, 1063
CfoI GCGC 1 cut(s) 857
Cfr10I RCCGGY 2 cut(s) 692, 998
Cfr13I GGNCC 3 cut(s) 172, 1159, 1165
Cfr9I CCCGGG 1 cut(s) 701
Csp6I GTAC 7 cut(s) 16, 35, 129, 823, 1061, 1087, 1108
CviAII CATG 4 cut(s) 13, 1105, 1207, 1265
CviQI GTAC 7 cut(s) 16, 35, 129, 823, 1061, 1087, 1108
DdeI CTNAG 3 cut(s) 96, 460, 502
DpnI GATC 5 cut(s) 251, 293, 653, 869, 938
DpnII GATC 5 cut(s) 249, 291, 651, 867, 936
Eam1104I CTCTTC 1 cut(s) 899
EarI CTCTTC 1 cut(s) 899
EciI GGCGGA 1 cut(s) 832
Ecl136II GAGCTC 1 cut(s) 111
Eco24I GRGCYC 2 cut(s) 113, 1148
Eco47I GGWCC 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 111
Eco72I CACGTG 1 cut(s) 1281
Eco88I CYCGRG 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 172
EcoRII CCWGG 2 cut(s) 168, 1161
EcoT38I GRGCYC 2 cut(s) 113, 1148
FaeI CATG 4 cut(s) 16, 1108, 1210, 1268
FaiI YATR 6 cut(s) 14, 974, 1106, 1208, 1221, 1266
FalI AAGNNNNNCTT 2 cut(s) 1319, 1351
FatI CATG 4 cut(s) 12, 1104, 1206, 1264
FbaI TGATCA 2 cut(s) 249, 291
FblI GTMKAC 2 cut(s) 579, 951
Fnu4HI GCNGC 6 cut(s) 828, 1094, 1097, 1100, 1238, 1241
FokI GGATG 1 cut(s) 526
FriOI GRGCYC 2 cut(s) 113, 1148
Fsp4HI GCNGC 6 cut(s) 828, 1094, 1097, 1100, 1238, 1241
GlaI GCGC 1 cut(s) 856
GluI GCNGC 6 cut(s) 828, 1094, 1097, 1100, 1238, 1241
GsaI CCCAGC 2 cut(s) 648, 1145
HaeIII GGCC 3 cut(s) 1002, 1161, 1166
HapII CCGG 3 cut(s) 693, 702, 999
HhaI GCGC 1 cut(s) 857
Hin1II CATG 4 cut(s) 16, 1108, 1210, 1268
Hin6I GCGC 1 cut(s) 855
HinP1I GCGC 1 cut(s) 855
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 1 cut(s) 1364
HinfI GANTC 6 cut(s) 98, 464, 741, 1069, 1133, 1269
HpaII CCGG 3 cut(s) 693, 702, 999
HphI GGTGA 4 cut(s) 516, 682, 988, 1361
Hpy166II GTNNAC 3 cut(s) 580, 952, 1041
Hpy188I TCNGA 5 cut(s) 29, 97, 463, 505, 685
Hpy188III TCNNGA 1 cut(s) 745
Hpy8I GTNNAC 3 cut(s) 580, 952, 1041
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 3 cut(s) 868, 1252, 1321
HpyCH4IV ACGT 3 cut(s) 1047, 1080, 1280
HpyCH4V TGCA 3 cut(s) 1057, 1066, 1339
HpyF10VI GCNNNNNNNGC 4 cut(s) 836, 1063, 1099, 1237
HpyF3I CTNAG 3 cut(s) 96, 460, 502
HpySE526I ACGT 3 cut(s) 1047, 1080, 1280
Hsp92II CATG 4 cut(s) 16, 1108, 1210, 1268
HspAI GCGC 1 cut(s) 855
Ksp22I TGATCA 2 cut(s) 249, 291
Kzo9I GATC 5 cut(s) 249, 291, 651, 867, 936
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 4 cut(s) 1080, 1083, 1086, 1249
LweI GCATC 1 cut(s) 912
MaeII ACGT 3 cut(s) 1047, 1080, 1280
MaeIII GTNAC 2 cut(s) 994, 1331
MalI GATC 5 cut(s) 251, 293, 653, 869, 938
MboI GATC 5 cut(s) 249, 291, 651, 867, 936
MhlI GDGCHC 3 cut(s) 113, 1148, 1233
MluCI AATT 9 cut(s) 149, 245, 287, 397, 403, 409, 484, 707, 760
MlyI GAGTC 2 cut(s) 107, 1063
MmeI TCCRAC 1 cut(s) 663
MroXI GAANNNNTTC 1 cut(s) 797
MseI TTAA 1 cut(s) 1369
MslI CAYNNNNRTG 1 cut(s) 1211
MspA1I CMGCKG 1 cut(s) 648
MspI CCGG 3 cut(s) 693, 702, 999
MspR9I CCNGG 4 cut(s) 170, 702, 703, 1163
MvaI CCWGG 2 cut(s) 170, 1163
MwoI GCNNNNNNNGC 4 cut(s) 836, 1063, 1099, 1237
NciI CCSGG 2 cut(s) 702, 703
NdeII GATC 5 cut(s) 249, 291, 651, 867, 936
NlaIII CATG 4 cut(s) 16, 1108, 1210, 1268
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 2 cut(s) 1053, 1339
NmuCI GTSAC 1 cut(s) 994
NspI RCATGY 1 cut(s) 1210
PciI ACATGT 1 cut(s) 1206
PcsI WCGNNNNNNNCGW 2 cut(s) 752, 843
PdmI GAANNNNTTC 1 cut(s) 797
PfeI GAWTC 4 cut(s) 464, 741, 1133, 1269
PflFI GACNNNGTC 1 cut(s) 190
PkrI GCNGC 6 cut(s) 829, 1095, 1098, 1101, 1239, 1242
PleI GAGTC 2 cut(s) 106, 1063
PmaCI CACGTG 1 cut(s) 1281
PmlI CACGTG 1 cut(s) 1281
PpsI GAGTC 2 cut(s) 106, 1063
Ppu21I YACGTR 1 cut(s) 1281
PpuMI RGGWCCY 1 cut(s) 172
PscI ACATGT 1 cut(s) 1206
Psp124BI GAGCTC 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 2 cut(s) 168, 1161
PspCI CACGTG 1 cut(s) 1281
PspFI CCCAGC 2 cut(s) 644, 1141
PspGI CCWGG 2 cut(s) 168, 1161
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 172, 1159, 1165
PspPPI RGGWCCY 1 cut(s) 172
PstI CTGCAG 2 cut(s) 1059, 1068
PstNI CAGNNNCTG 2 cut(s) 175, 1063
PsyI GACNNNGTC 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 648
RsaI GTAC 7 cut(s) 17, 36, 130, 824, 1062, 1088, 1109
RsaNI GTAC 7 cut(s) 16, 35, 129, 823, 1061, 1087, 1108
RseI CAYNNNNRTG 1 cut(s) 1211
SacI GAGCTC 1 cut(s) 113
SalI GTCGAC 1 cut(s) 578
SaqAI TTAA 1 cut(s) 1369
SatI GCNGC 6 cut(s) 828, 1094, 1097, 1100, 1238, 1241
Sau3AI GATC 5 cut(s) 249, 291, 651, 867, 936
Sau96I GGNCC 3 cut(s) 172, 1159, 1165
SbfI CCTGCAGG 1 cut(s) 1059
SchI GAGTC 2 cut(s) 107, 1063
ScrFI CCNGG 4 cut(s) 170, 702, 703, 1163
SdaI CCTGCAGG 1 cut(s) 1059
SduI GDGCHC 3 cut(s) 113, 1148, 1233
SfaNI GCATC 1 cut(s) 912
SfcI CTRYAG 2 cut(s) 1055, 1064
SinI GGWCC 1 cut(s) 172
SmaI CCCGGG 1 cut(s) 703
SmiMI CAYNNNNRTG 1 cut(s) 1211
SmlI CTYRAG 3 cut(s) 19, 1013, 1232
SmoI CTYRAG 3 cut(s) 19, 1013, 1232
Sse8387I CCTGCAGG 1 cut(s) 1059
Sse9I AATT 9 cut(s) 149, 245, 287, 397, 403, 409, 484, 707, 760
SsiI CCGC 5 cut(s) 828, 843, 901, 1241, 1311
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 4 cut(s) 168, 700, 701, 1161
TaiI ACGT 3 cut(s) 1050, 1083, 1283
TasI AATT 9 cut(s) 149, 245, 287, 397, 403, 409, 484, 707, 760
TatI WGTACW 3 cut(s) 15, 128, 1107
TauI GCSGC 2 cut(s) 830, 1243
TfiI GAWTC 4 cut(s) 464, 741, 1133, 1269
Tru1I TTAA 1 cut(s) 1369
Tru9I TTAA 1 cut(s) 1369
TseFI GTSAC 1 cut(s) 994
TseI GCWGC 4 cut(s) 1093, 1096, 1099, 1237
Tsp45I GTSAC 1 cut(s) 994
TspDTI ATGAA 4 cut(s) 499, 1187, 1208, 1253
TspGWI ACGGA 1 cut(s) 91
TspMI CCCGGG 1 cut(s) 701
Tth111I GACNNNGTC 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 172
XapI RAATTY 2 cut(s) 484, 707
XceI RCATGY 1 cut(s) 1210
XmaI CCCGGG 1 cut(s) 701
XmiI GTMKAC 2 cut(s) 579, 951
XmnI GAANNNNTTC 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.