Rh2DG256700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014549
Physical Location & Seq
Reverse (-)
27272942 .. 27275740
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG256700.1

Sequence Viewer

Length: 1311 bp
ATGGCTCCACTCATGTACTTCAAGTTCAGAAAGGTACTTTTGCTAGTTTCTCTTGTTGTGTTCCTCTTTTATTCTACTCTGCTCCTTTGTGCCGCAGAAGAAGATGAGAAACTGCCTCGAATTCAGAGCTCATCACAGTTTTTGGCTTCAAGGAAAATGTTGGAATTAGATGAAGAAGATGAAGAAGTCCAGCCTAAGAAGATCAAGACCAAAACCACCACCTCAACTCCCCAATCCTCCAAAAACCAAACCAAGCTGCTCAAGCCACTTAAACCCAACCTATCATCCAAGAACCAAACTAAGCTCATCAAACCAAGCACCAATCCAACCAAGTCATCATCATCATCATCACCACCCAGTATCAAGATTGGCCTCAAGAAGCTCAATTCCACTTCCAAACCCTCAAACTCCACCAAACCCACTTCACTGTCATCCAAGAAGACCTCAGATCTAGCCAAACTAACCTCCCCCAAGAACAAAACAACCAAACCCACCACCACAAAACAACCTCAAACTCCCAAACCAATCCAAAAAACACAGAAAACCCCCACCCCACAAAAACCCACCAAGAAACCAATCCAACCCAGCTGGATCGAACAAGAAGACGACACCGACTTCGTCTCCGACTTCACAGACTTACCCGGCCAATTCCAACAGAGTCTGATCCCAGACCTGGAGAAAATCTCAACCACATCAAAAATCTACCTCACCCAAGCCAACAAGCAAATGACCACCAGGTTCAAACCACTCGTCGGAAATAAATACGCCCCCACCATCGCTTCCATTGTCTCCTGCGCATTCCTACTTATCCCATTGCTCCTAGTCTCTCTCCTCTTCAACCGCATCAAAGCCTACTTTTCCCTCCAAAAGCTTCTCATCTTCATCCAAGTCTACCTCTCCATCTACTTCACAATCCTCTGCCTCTCTTCCTTGGTCACTGGTCTCGAGCCCCTCAAGTTCTTCTACGCTACTTCGCAGTCCACCTACATCTGCCTCCAGGTGCTGCAGACTCTCGGCTACGTGCTCTATCTCCTCCTCCTCATCATGTACTTGATCCTGGTTTTCTCGACCGACTCCGGGCTGGGCTCGAAGATGTTGGGCCTGGCCCAGACTTTTCTCGGGTTCGCGGTCGGGCTGCATTACTACATGTCGGTGTTTCACAGGGCGGTGCTGCGGCAGCCGCCGAAGACTAATTGGAAGGTGCACGGGATATACGCCATGTGTTACCTTGTGATTTGTCTGTTTGCCACGGCGGAGAGGAGGAAGAAGGCTTACTTGGAAGAAGGTGGTGAAGAGGGCAAGAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

436

Amino Acids

49.03

Weight (kDa)

9.75

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39840
malus_domestica MD01G1028100.v1.1 MD15G1325300.v1.1
prunus_persica Prupe.6G184700_v2.0.a1
pyrus_communis pycom01g05640
rosa_chinensis RchiOBHm_Chr2g0114541
rosa_laevigata RLG00000018113
rosa_multiflora Rmu_sc0000370.1_g000001 Rmu_sc0000371.1_g000011 Rmu_sc0027184.1_g000005
rosa_roxburghii Rroxscaffold_2G00130280
rosa_rugosa Rorug02G0190700
rosa_samantha Rh2AG248600 Rh2BG258200 Rh2CG252000 Rh2DG256700
rosa_wichuraiana Rw2G019330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 796
AccI GTMKAC 1 cut(s) 891
AccII CGCG 1 cut(s) 1127
AciI CCGC 7 cut(s) 93, 841, 1127, 1166, 1174, 1181, 1253
AclWI GGATC 3 cut(s) 599, 658, 1048
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 1 cut(s) 120
AfaI GTAC 3 cut(s) 17, 36, 1049
AfiI CCNNNNNNNGG 3 cut(s) 673, 752, 1077
AflIII ACRYGT 1 cut(s) 1146
AgsI TTSAA 4 cut(s) 22, 150, 742, 838
AjnI CCWGG 5 cut(s) 672, 734, 996, 1056, 1101
AjuI GAANNNNNNNTTGG 2 cut(s) 1259, 1291
AluBI AGCT 6 cut(s) 129, 256, 304, 382, 588, 871
AluI AGCT 6 cut(s) 129, 256, 304, 382, 588, 871
Alw21I GWGCWC 3 cut(s) 131, 1026, 1206
Alw26I GTCTC 4 cut(s) 625, 793, 829, 947
Alw44I GTGCAC 1 cut(s) 1202
AlwI GGATC 3 cut(s) 599, 658, 1048
AlwNI CAGNNNCTG 2 cut(s) 661, 1003
Ama87I CYCGRG 2 cut(s) 944, 1118
AoxI GGCC 4 cut(s) 370, 643, 1099, 1104
ApaLI GTGCAC 1 cut(s) 1202
ApeKI GCWGC 5 cut(s) 256, 1003, 1135, 1171, 1177
ApoI RAATTY 1 cut(s) 120
ArsI GACNNNNNNTTYG 4 cut(s) 599, 631, 735, 767
AspLEI GCGC 1 cut(s) 797
AspS9I GGNCC 2 cut(s) 1099, 1105
AsuC2I CCSGG 2 cut(s) 642, 1078
AsuHPI GGTGA 3 cut(s) 342, 700, 1301
AvaI CYCGRG 2 cut(s) 944, 1118
BaeGI GKGCMC 1 cut(s) 1206
BanII GRGCYC 3 cut(s) 131, 951, 1088
BarI GAAGNNNNNNTAC 2 cut(s) 1256, 1288
BbsI GAAGAC 3 cut(s) 446, 609, 1193
Bbv12I GWGCWC 3 cut(s) 131, 1026, 1206
BbvI GCAGC 5 cut(s) 243, 990, 1122, 1158, 1189
BccI CCATC 2 cut(s) 782, 908
BceAI ACGGC 1 cut(s) 1266
BciT130I CCWGG 5 cut(s) 674, 736, 998, 1058, 1103
BcnI CCSGG 2 cut(s) 642, 1078
BcoDI GTCTC 4 cut(s) 625, 793, 829, 947
BfaI CTAG 3 cut(s) 44, 452, 821
BfmI CTRYAG 1 cut(s) 1004
BglII AGATCT 1 cut(s) 448
BisI GCNGC 8 cut(s) 93, 257, 1004, 1136, 1172, 1175, 1178, 1181
BlsI GCNGC 8 cut(s) 94, 258, 1005, 1137, 1173, 1176, 1179, 1182
Bme1390I CCNGG 7 cut(s) 642, 674, 736, 998, 1058, 1078, 1103
BmeT110I CYCGRG 2 cut(s) 944, 1118
BmgT120I GGNCC 2 cut(s) 1099, 1105
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 7 cut(s) 642, 674, 736, 998, 1058, 1078, 1103
BmrI ACTGGG 1 cut(s) 351
BmsI GCATC 1 cut(s) 852
BmuI ACTGGG 1 cut(s) 351
BpiI GAAGAC 3 cut(s) 446, 609, 1193
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 2 cut(s) 695, 980
BpuEI CTTGAG 3 cut(s) 245, 359, 938
BpuMI CCSGG 2 cut(s) 642, 1078
BsaAI YACGTR 1 cut(s) 1021
BsaI GGTCTC 1 cut(s) 947
BsaJI CCNNGG 2 cut(s) 930, 1248
BsaXI ACNNNNNCTCC 4 cut(s) 211, 241, 605, 635
Bsc4I CCNNNNNNNGG 3 cut(s) 673, 752, 1077
Bse1I ACTGG 2 cut(s) 357, 943
Bse3DI GCAATG 1 cut(s) 812
BseBI CCWGG 5 cut(s) 674, 736, 998, 1058, 1103
BseDI CCNNGG 2 cut(s) 930, 1248
BseGI GGATG 3 cut(s) 284, 431, 882
BseLI CCNNNNNNNGG 3 cut(s) 673, 752, 1077
BseMI GCAATG 1 cut(s) 812
BseMII CTCAG 1 cut(s) 459
BseNI ACTGG 2 cut(s) 357, 943
BseRI GAGGAG 5 cut(s) 821, 1022, 1025, 1028, 1273
BseSI GKGCMC 1 cut(s) 1206
BseXI GCAGC 5 cut(s) 243, 990, 1122, 1158, 1189
BseYI CCCAGC 2 cut(s) 584, 1081
Bsh1236I CGCG 1 cut(s) 1127
Bsh1285I CGRYCG 2 cut(s) 1071, 1131
BshFI GGCC 4 cut(s) 372, 645, 1101, 1106
BsiEI CGRYCG 2 cut(s) 1071, 1131
BsiHKAI GWGCWC 3 cut(s) 131, 1026, 1206
BsiHKCI CYCGRG 2 cut(s) 944, 1118
BsiSI CCGG 2 cut(s) 642, 1077
BslI CCNNNNNNNGG 3 cut(s) 673, 752, 1077
BsmAI GTCTC 4 cut(s) 625, 793, 829, 947
BsmBI CGTCTC 1 cut(s) 625
BsmI GAATGC 1 cut(s) 797
BsnI GGCC 4 cut(s) 372, 645, 1101, 1106
Bso31I GGTCTC 1 cut(s) 947
BsoBI CYCGRG 2 cut(s) 944, 1118
Bsp1286I GDGCHC 5 cut(s) 131, 951, 1026, 1088, 1206
Bsp143I GATC 5 cut(s) 201, 448, 591, 663, 1053
BspACI CCGC 7 cut(s) 93, 841, 1127, 1166, 1174, 1181, 1253
BspANI GGCC 4 cut(s) 372, 645, 1101, 1106
BspCNI CTCAG 1 cut(s) 458
BspFNI CGCG 1 cut(s) 1127
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 1008
BspPI GGATC 3 cut(s) 599, 658, 1048
BspTNI GGTCTC 1 cut(s) 947
BsrDI GCAATG 1 cut(s) 812
BsrI ACTGG 2 cut(s) 357, 943
BssECI CCNNGG 2 cut(s) 930, 1248
BssMI GATC 5 cut(s) 201, 448, 591, 663, 1053
BssT1I CCWWGG 1 cut(s) 930
Bst2UI CCWGG 5 cut(s) 674, 736, 998, 1058, 1103
Bst4CI ACNGT 2 cut(s) 138, 429
Bst6I CTCTTC 3 cut(s) 839, 931, 1287
BstBAI YACGTR 1 cut(s) 1021
BstDEI CTNAG 3 cut(s) 195, 300, 445
BstDSI CCRYGG 1 cut(s) 1248
BstF5I GGATG 3 cut(s) 284, 431, 882
BstFNI CGCG 1 cut(s) 1127
BstHHI GCGC 1 cut(s) 797
BstKTI GATC 5 cut(s) 204, 451, 594, 666, 1056
BstMAI GTCTC 4 cut(s) 625, 793, 829, 947
BstMBI GATC 5 cut(s) 201, 448, 591, 663, 1053
BstMCI CGRYCG 2 cut(s) 1071, 1131
BstMWI GCNNNNNNNGC 3 cut(s) 262, 1177, 1180
BstNI CCWGG 5 cut(s) 674, 736, 998, 1058, 1103
BstNSI RCATGY 1 cut(s) 1150
BstSCI CCNGG 7 cut(s) 640, 672, 734, 996, 1056, 1076, 1101
BstSFI CTRYAG 1 cut(s) 1004
BstSLI GKGCMC 1 cut(s) 1206
BstUI CGCG 1 cut(s) 1127
BstV1I GCAGC 5 cut(s) 243, 990, 1122, 1158, 1189
BstV2I GAAGAC 3 cut(s) 446, 609, 1193
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 4 cut(s) 372, 645, 1101, 1106
BtgI CCRYGG 1 cut(s) 1248
BtgZI GCGATG 1 cut(s) 760
BtsCI GGATG 3 cut(s) 284, 431, 882
BtsIMutI CAGTG 2 cut(s) 425, 936
CaiI CAGNNNCTG 2 cut(s) 661, 1003
CfoI GCGC 1 cut(s) 797
Cfr13I GGNCC 2 cut(s) 1099, 1105
CsiI ACCWGGT 1 cut(s) 734
Csp6I GTAC 3 cut(s) 16, 35, 1048
CviAII CATG 4 cut(s) 13, 1045, 1147, 1219
CviQI GTAC 3 cut(s) 16, 35, 1048
DdeI CTNAG 3 cut(s) 195, 300, 445
DpnI GATC 5 cut(s) 203, 450, 593, 665, 1055
DpnII GATC 5 cut(s) 201, 448, 591, 663, 1053
EaeI YGGCCR 1 cut(s) 643
Eam1104I CTCTTC 3 cut(s) 839, 931, 1287
EarI CTCTTC 3 cut(s) 839, 931, 1287
EciI GGCGGA 1 cut(s) 1268
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 930
Eco24I GRGCYC 3 cut(s) 131, 951, 1088
Eco31I GGTCTC 1 cut(s) 947
Eco53kI GAGCTC 1 cut(s) 129
Eco88I CYCGRG 2 cut(s) 944, 1118
EcoICRI GAGCTC 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 5 cut(s) 672, 734, 996, 1056, 1101
EcoT14I CCWWGG 1 cut(s) 930
EcoT38I GRGCYC 3 cut(s) 131, 951, 1088
ErhI CCWWGG 1 cut(s) 930
Esp3I CGTCTC 1 cut(s) 625
FaeI CATG 4 cut(s) 16, 1048, 1150, 1222
FaiI YATR 5 cut(s) 14, 1046, 1148, 1213, 1220
FalI AAGNNNNNCTT 2 cut(s) 1259, 1291
FatI CATG 4 cut(s) 12, 1044, 1146, 1218
FblI GTMKAC 1 cut(s) 891
Fnu4HI GCNGC 8 cut(s) 93, 257, 1004, 1136, 1172, 1175, 1178, 1181
FokI GGATG 3 cut(s) 271, 418, 869
FriOI GRGCYC 3 cut(s) 131, 951, 1088
Fsp4HI GCNGC 8 cut(s) 93, 257, 1004, 1136, 1172, 1175, 1178, 1181
FspBI CTAG 3 cut(s) 44, 452, 821
FspI TGCGCA 1 cut(s) 796
GlaI GCGC 1 cut(s) 796
GluI GCNGC 8 cut(s) 93, 257, 1004, 1136, 1172, 1175, 1178, 1181
GsaI CCCAGC 2 cut(s) 588, 1085
GsuI CTGGAG 2 cut(s) 695, 980
HaeIII GGCC 4 cut(s) 372, 645, 1101, 1106
HapII CCGG 2 cut(s) 642, 1077
HhaI GCGC 1 cut(s) 797
Hin1II CATG 4 cut(s) 16, 1048, 1150, 1222
Hin6I GCGC 1 cut(s) 795
HinP1I GCGC 1 cut(s) 795
HindIII AAGCTT 1 cut(s) 869
HinfI GANTC 3 cut(s) 658, 1009, 1073
HpaII CCGG 2 cut(s) 642, 1077
HphI GGTGA 3 cut(s) 342, 700, 1301
Hpy166II GTNNAC 3 cut(s) 892, 981, 1204
Hpy188I TCNGA 6 cut(s) 29, 126, 448, 625, 663, 755
Hpy188III TCNNGA 5 cut(s) 205, 364, 376, 944, 1066
Hpy8I GTNNAC 3 cut(s) 892, 981, 1204
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 3 cut(s) 1192, 1261, 1277
HpyCH4III ACNGT 2 cut(s) 138, 429
HpyCH4IV ACGT 1 cut(s) 1020
HpyCH4V TGCA 3 cut(s) 1006, 1138, 1204
HpyF10VI GCNNNNNNNGC 3 cut(s) 262, 1177, 1180
HpyF3I CTNAG 3 cut(s) 195, 300, 445
HpySE526I ACGT 1 cut(s) 1020
Hsp92II CATG 4 cut(s) 16, 1048, 1150, 1222
HspAI GCGC 1 cut(s) 795
Kzo9I GATC 5 cut(s) 201, 448, 591, 663, 1053
LmnI GCTCC 3 cut(s) 10, 87, 822
Lsp1109I GCAGC 5 cut(s) 243, 990, 1122, 1158, 1189
LweI GCATC 1 cut(s) 852
MabI ACCWGGT 1 cut(s) 734
MaeI CTAG 3 cut(s) 44, 452, 821
MaeII ACGT 1 cut(s) 1020
MaeIII GTNAC 2 cut(s) 934, 1223
MalI GATC 5 cut(s) 203, 450, 593, 665, 1055
MboI GATC 5 cut(s) 201, 448, 591, 663, 1053
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 5 cut(s) 131, 951, 1026, 1088, 1206
MluCI AATT 5 cut(s) 120, 164, 385, 647, 1192
MlyI GAGTC 3 cut(s) 667, 1003, 1067
MmeI TCCRAC 6 cut(s) 141, 350, 604, 648, 676, 733
MseI TTAA 1 cut(s) 270
MslI CAYNNNNRTG 1 cut(s) 1151
MspA1I CMGCKG 1 cut(s) 588
MspI CCGG 2 cut(s) 642, 1077
MspR9I CCNGG 7 cut(s) 642, 674, 736, 998, 1058, 1078, 1103
Mva1269I GAATGC 1 cut(s) 797
MvaI CCWGG 5 cut(s) 674, 736, 998, 1058, 1103
MvnI CGCG 1 cut(s) 1127
MwoI GCNNNNNNNGC 3 cut(s) 262, 1177, 1180
NciI CCSGG 2 cut(s) 642, 1078
NdeII GATC 5 cut(s) 201, 448, 591, 663, 1053
NlaIII CATG 4 cut(s) 16, 1048, 1150, 1222
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 993
NmuCI GTSAC 1 cut(s) 934
NsbI TGCGCA 1 cut(s) 796
NspI RCATGY 1 cut(s) 1150
PaeR7I CTCGAG 1 cut(s) 944
PciI ACATGT 1 cut(s) 1146
PctI GAATGC 1 cut(s) 797
PflFI GACNNNGTC 1 cut(s) 617
PkrI GCNGC 8 cut(s) 94, 258, 1005, 1137, 1173, 1176, 1179, 1182
PleI GAGTC 3 cut(s) 666, 1003, 1067
PpsI GAGTC 3 cut(s) 666, 1003, 1067
Ppu21I YACGTR 1 cut(s) 1021
PscI ACATGT 1 cut(s) 1146
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 5 cut(s) 672, 734, 996, 1056, 1101
PspFI CCCAGC 2 cut(s) 584, 1081
PspGI CCWGG 5 cut(s) 672, 734, 996, 1056, 1101
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 1099, 1105
PsrI GAACNNNNNNTAC 2 cut(s) 8, 40
PstI CTGCAG 1 cut(s) 1008
PstNI CAGNNNCTG 2 cut(s) 661, 1003
PsuI RGATCY 1 cut(s) 448
PsyI GACNNNGTC 1 cut(s) 617
PvuII CAGCTG 1 cut(s) 588
RsaI GTAC 3 cut(s) 17, 36, 1049
RsaNI GTAC 3 cut(s) 16, 35, 1048
RseI CAYNNNNRTG 1 cut(s) 1151
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 8 cut(s) 93, 257, 1004, 1136, 1172, 1175, 1178, 1181
Sau3AI GATC 5 cut(s) 201, 448, 591, 663, 1053
Sau96I GGNCC 2 cut(s) 1099, 1105
SchI GAGTC 3 cut(s) 667, 1003, 1067
ScrFI CCNGG 7 cut(s) 642, 674, 736, 998, 1058, 1078, 1103
SduI GDGCHC 5 cut(s) 131, 951, 1026, 1088, 1206
SexAI ACCWGGT 1 cut(s) 734
SfaNI GCATC 1 cut(s) 852
SfcI CTRYAG 1 cut(s) 1004
Sfr274I CTCGAG 1 cut(s) 944
SlaI CTCGAG 1 cut(s) 944
SmiMI CAYNNNNRTG 1 cut(s) 1151
SmlI CTYRAG 4 cut(s) 260, 374, 944, 953
SmoI CTYRAG 4 cut(s) 260, 374, 944, 953
Sse9I AATT 5 cut(s) 120, 164, 385, 647, 1192
SsiI CCGC 7 cut(s) 93, 841, 1127, 1166, 1174, 1181, 1253
SspMI CTAG 3 cut(s) 44, 452, 821
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 7 cut(s) 640, 672, 734, 996, 1056, 1076, 1101
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 2 cut(s) 138, 429
TaiI ACGT 1 cut(s) 1023
TaqI TCGA 5 cut(s) 118, 594, 945, 1067, 1088
TaqII GACCGA 1 cut(s) 1085
TasI AATT 5 cut(s) 120, 164, 385, 647, 1192
TatI WGTACW 2 cut(s) 15, 1047
TauI GCSGC 3 cut(s) 95, 1177, 1183
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 2 cut(s) 432, 943
TseFI GTSAC 1 cut(s) 934
TseI GCWGC 5 cut(s) 256, 1003, 1135, 1171, 1177
Tsp45I GTSAC 1 cut(s) 934
TspDTI ATGAA 3 cut(s) 186, 195, 871
TspRI CASTG 2 cut(s) 432, 943
Tth111I GACNNNGTC 1 cut(s) 617
VneI GTGCAC 1 cut(s) 1202
XapI RAATTY 1 cut(s) 120
XceI RCATGY 1 cut(s) 1150
XhoI CTCGAG 1 cut(s) 944
XmiI GTMKAC 1 cut(s) 891
XspI CTAG 3 cut(s) 44, 452, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.