Rmu_sc0027184.1_g000005

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027184.1
OG0014549
Physical Location & Seq
Forward (+)
14410 .. 15750
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027184.1_g000005.1.cds

Sequence Viewer

Length: 1341 bp
atggctccactcatatacttcaagttcagaaaggtacttttgctagtttctcttgttgtgttcctcttttcttctactctgctcctttgtgccgcagaagaagatgagaaactgcctcgaattcagagctcatcacagtttttggcttcaaggaaaatgttggaattagatgaagaagtccagcctaagaagatcaagaccaaaaccaccacctcaactccccaatcctccaaaaaccaaaccaagcttatcaaacccacttcatcatcctccaaaaaccaaaccaagctgctcaagccacttaaacccaacctatcatccaagaaccagaccaagctcatcaaaccaagcaccaatcaaaccaagtcatcaccgtcatcaccacccagtatcaagattggcctcaagaagctcaattccacttccaaaccctcaaactcccccaaaccgacttcactatcatccaagaagacctcagatctagccaaactaacctcccccaagaacaaaacaaccaaacccaccaccacaaaacaacctcaaactcccaaaccaatcccaaaaacacagaaaacccccaccccacaaaaacccaccaagaaaccaatccaacccagctggatcgaacaagaagacgacaccgacttcgtctccgacttcacagacttacccggccaattccaacagacactgatcccagacctggagaaaatctcaaccacatcaaaaatctacctcacccaagccaacaagcaaatgaccaccaggttcaaaccactcgtcggaaacaaatacgcccccaccattgcttccatagtctcctgcgcattcctacttatcccattgctcctagtctctctcctcttcaaccgcatcaaagcctacttttccctccaaaagctcctcatcttcatccaagtctacctctccatctacttctcaatcctctgcctctcttccttggtcactggcctcgagcccctcaagtttttctacgctacttcgcagtccacctacatctgcctccaggtgctgcagactctcggctacgtgctctatctcctcctcctcatcatgtacttgatcctggttttctcgaccgactccgggctgggctcgaagatgttgggcctggcccagacttttctcgggttcgcggtcgggctgcattactacatgtcggtgtttcacagggcggtgctgcggcagccgccgaagactaattggaaggtgcacgggatatacgccgtgtgttaccttgtgatttgtctgtttgccacggcggagaggaggaagaaggcttacttggaagaaggtggtgaagagggcaagaagaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

446

Amino Acids

49.95

Weight (kDa)

9.89

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39840
malus_domestica MD01G1028100.v1.1 MD15G1325300.v1.1
prunus_persica Prupe.6G184700_v2.0.a1
pyrus_communis pycom01g05640
rosa_chinensis RchiOBHm_Chr2g0114541
rosa_laevigata RLG00000018113
rosa_multiflora Rmu_sc0000370.1_g000001 Rmu_sc0000371.1_g000011 Rmu_sc0027184.1_g000005
rosa_roxburghii Rroxscaffold_2G00130280
rosa_rugosa Rorug02G0190700
rosa_samantha Rh2AG248600 Rh2BG258200 Rh2CG252000 Rh2DG256700
rosa_wichuraiana Rw2G019330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 826
AccI GTMKAC 1 cut(s) 921
AccII CGCG 1 cut(s) 1157
AciI CCGC 7 cut(s) 93, 871, 1157, 1196, 1204, 1211, 1283
AclWI GGATC 3 cut(s) 629, 688, 1078
AcoI YGGCCR 1 cut(s) 673
AcsI RAATTY 1 cut(s) 120
AfaI GTAC 2 cut(s) 36, 1079
AfiI CCNNNNNNNGG 3 cut(s) 703, 782, 1107
AflIII ACRYGT 1 cut(s) 1176
AgsI TTSAA 4 cut(s) 22, 150, 772, 868
AjnI CCWGG 5 cut(s) 702, 764, 1026, 1086, 1131
AjuI GAANNNNNNNTTGG 2 cut(s) 1289, 1321
AluBI AGCT 7 cut(s) 129, 247, 289, 337, 412, 618, 901
AluI AGCT 7 cut(s) 129, 247, 289, 337, 412, 618, 901
Alw21I GWGCWC 3 cut(s) 131, 1056, 1236
Alw26I GTCTC 3 cut(s) 655, 823, 859
Alw44I GTGCAC 1 cut(s) 1232
AlwI GGATC 3 cut(s) 629, 688, 1078
AlwNI CAGNNNCTG 2 cut(s) 691, 1033
Ama87I CYCGRG 2 cut(s) 974, 1148
AoxI GGCC 5 cut(s) 400, 673, 970, 1129, 1134
ApaLI GTGCAC 1 cut(s) 1232
ApeKI GCWGC 5 cut(s) 289, 1033, 1165, 1201, 1207
ApoI RAATTY 1 cut(s) 120
ArsI GACNNNNNNTTYG 4 cut(s) 629, 661, 765, 797
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 2 cut(s) 1129, 1135
AsuC2I CCSGG 2 cut(s) 672, 1108
AsuHPI GGTGA 4 cut(s) 363, 372, 730, 1331
AvaI CYCGRG 2 cut(s) 974, 1148
BaeGI GKGCMC 1 cut(s) 1236
BanII GRGCYC 3 cut(s) 131, 981, 1118
BarI GAAGNNNNNNTAC 2 cut(s) 1286, 1318
BbsI GAAGAC 3 cut(s) 476, 639, 1223
Bbv12I GWGCWC 3 cut(s) 131, 1056, 1236
BbvI GCAGC 5 cut(s) 276, 1020, 1152, 1188, 1219
BccI CCATC 1 cut(s) 938
BceAI ACGGC 2 cut(s) 1232, 1296
BciT130I CCWGG 5 cut(s) 704, 766, 1028, 1088, 1133
BcnI CCSGG 2 cut(s) 672, 1108
BcoDI GTCTC 3 cut(s) 655, 823, 859
BfaI CTAG 3 cut(s) 44, 482, 851
BfmI CTRYAG 1 cut(s) 1034
BglII AGATCT 1 cut(s) 478
BisI GCNGC 8 cut(s) 93, 290, 1034, 1166, 1202, 1205, 1208, 1211
BlsI GCNGC 8 cut(s) 94, 291, 1035, 1167, 1203, 1206, 1209, 1212
Bme1390I CCNGG 7 cut(s) 672, 704, 766, 1028, 1088, 1108, 1133
BmeT110I CYCGRG 2 cut(s) 974, 1148
BmgT120I GGNCC 2 cut(s) 1129, 1135
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 7 cut(s) 672, 704, 766, 1028, 1088, 1108, 1133
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 882
BmuI ACTGGG 1 cut(s) 381
BpiI GAAGAC 3 cut(s) 476, 639, 1223
BplI GAGNNNNNCTC 2 cut(s) 698, 730
BpmI CTGGAG 2 cut(s) 725, 1010
BpuEI CTTGAG 3 cut(s) 278, 389, 968
BpuMI CCSGG 2 cut(s) 672, 1108
BsaAI YACGTR 1 cut(s) 1051
BsaJI CCNNGG 2 cut(s) 960, 1278
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 635, 665
Bsc4I CCNNNNNNNGG 3 cut(s) 703, 782, 1107
Bse1I ACTGG 2 cut(s) 387, 973
Bse3DI GCAATG 2 cut(s) 804, 842
BseBI CCWGG 5 cut(s) 704, 766, 1028, 1088, 1133
BseDI CCNNGG 2 cut(s) 960, 1278
BseGI GGATG 4 cut(s) 266, 317, 461, 912
BseLI CCNNNNNNNGG 3 cut(s) 703, 782, 1107
BseMI GCAATG 2 cut(s) 804, 842
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 2 cut(s) 387, 973
BseRI GAGGAG 6 cut(s) 851, 893, 1052, 1055, 1058, 1303
BseSI GKGCMC 1 cut(s) 1236
BseXI GCAGC 5 cut(s) 276, 1020, 1152, 1188, 1219
BseYI CCCAGC 2 cut(s) 614, 1111
Bsh1236I CGCG 1 cut(s) 1157
Bsh1285I CGRYCG 2 cut(s) 1101, 1161
BshFI GGCC 5 cut(s) 402, 675, 972, 1131, 1136
BsiEI CGRYCG 2 cut(s) 1101, 1161
BsiHKAI GWGCWC 3 cut(s) 131, 1056, 1236
BsiHKCI CYCGRG 2 cut(s) 974, 1148
BsiSI CCGG 2 cut(s) 672, 1107
BslI CCNNNNNNNGG 3 cut(s) 703, 782, 1107
BsmAI GTCTC 3 cut(s) 655, 823, 859
BsmBI CGTCTC 1 cut(s) 655
BsmI GAATGC 1 cut(s) 827
BsnI GGCC 5 cut(s) 402, 675, 972, 1131, 1136
BsoBI CYCGRG 2 cut(s) 974, 1148
Bsp1286I GDGCHC 5 cut(s) 131, 981, 1056, 1118, 1236
Bsp143I GATC 5 cut(s) 192, 478, 621, 693, 1083
BspACI CCGC 7 cut(s) 93, 871, 1157, 1196, 1204, 1211, 1283
BspANI GGCC 5 cut(s) 402, 675, 972, 1131, 1136
BspCNI CTCAG 1 cut(s) 488
BspFNI CGCG 1 cut(s) 1157
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 1038
BspPI GGATC 3 cut(s) 629, 688, 1078
BsrDI GCAATG 2 cut(s) 804, 842
BsrI ACTGG 2 cut(s) 387, 973
BssECI CCNNGG 2 cut(s) 960, 1278
BssMI GATC 5 cut(s) 192, 478, 621, 693, 1083
BssT1I CCWWGG 1 cut(s) 960
Bst2UI CCWGG 5 cut(s) 704, 766, 1028, 1088, 1133
Bst4CI ACNGT 2 cut(s) 138, 375
Bst6I CTCTTC 3 cut(s) 869, 961, 1317
BstBAI YACGTR 1 cut(s) 1051
BstDEI CTNAG 2 cut(s) 186, 475
BstDSI CCRYGG 1 cut(s) 1278
BstF5I GGATG 4 cut(s) 266, 317, 461, 912
BstFNI CGCG 1 cut(s) 1157
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 5 cut(s) 195, 481, 624, 696, 1086
BstMAI GTCTC 3 cut(s) 655, 823, 859
BstMBI GATC 5 cut(s) 192, 478, 621, 693, 1083
BstMCI CGRYCG 2 cut(s) 1101, 1161
BstMWI GCNNNNNNNGC 3 cut(s) 295, 1207, 1210
BstNI CCWGG 5 cut(s) 704, 766, 1028, 1088, 1133
BstNSI RCATGY 1 cut(s) 1180
BstSCI CCNGG 7 cut(s) 670, 702, 764, 1026, 1086, 1106, 1131
BstSFI CTRYAG 1 cut(s) 1034
BstSLI GKGCMC 1 cut(s) 1236
BstUI CGCG 1 cut(s) 1157
BstV1I GCAGC 5 cut(s) 276, 1020, 1152, 1188, 1219
BstV2I GAAGAC 3 cut(s) 476, 639, 1223
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 5 cut(s) 402, 675, 972, 1131, 1136
BtgI CCRYGG 1 cut(s) 1278
BtsCI GGATG 4 cut(s) 266, 317, 461, 912
BtsIMutI CAGTG 2 cut(s) 689, 966
CaiI CAGNNNCTG 2 cut(s) 691, 1033
CfoI GCGC 1 cut(s) 827
Cfr13I GGNCC 2 cut(s) 1129, 1135
CsiI ACCWGGT 1 cut(s) 764
Csp6I GTAC 2 cut(s) 35, 1078
CviAII CATG 2 cut(s) 1075, 1177
CviQI GTAC 2 cut(s) 35, 1078
DdeI CTNAG 2 cut(s) 186, 475
DpnI GATC 5 cut(s) 194, 480, 623, 695, 1085
DpnII GATC 5 cut(s) 192, 478, 621, 693, 1083
EaeI YGGCCR 1 cut(s) 673
Eam1104I CTCTTC 3 cut(s) 869, 961, 1317
EarI CTCTTC 3 cut(s) 869, 961, 1317
EciI GGCGGA 1 cut(s) 1298
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 960
Eco24I GRGCYC 3 cut(s) 131, 981, 1118
Eco53kI GAGCTC 1 cut(s) 129
Eco88I CYCGRG 2 cut(s) 974, 1148
EcoICRI GAGCTC 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 5 cut(s) 702, 764, 1026, 1086, 1131
EcoT14I CCWWGG 1 cut(s) 960
EcoT38I GRGCYC 3 cut(s) 131, 981, 1118
ErhI CCWWGG 1 cut(s) 960
Esp3I CGTCTC 1 cut(s) 655
FaeI CATG 2 cut(s) 1078, 1180
FaiI YATR 6 cut(s) 14, 16, 815, 1076, 1178, 1243
FalI AAGNNNNNCTT 2 cut(s) 1289, 1321
FatI CATG 2 cut(s) 1074, 1176
FblI GTMKAC 1 cut(s) 921
Fnu4HI GCNGC 8 cut(s) 93, 290, 1034, 1166, 1202, 1205, 1208, 1211
FokI GGATG 4 cut(s) 253, 304, 448, 899
FriOI GRGCYC 3 cut(s) 131, 981, 1118
Fsp4HI GCNGC 8 cut(s) 93, 290, 1034, 1166, 1202, 1205, 1208, 1211
FspBI CTAG 3 cut(s) 44, 482, 851
FspI TGCGCA 1 cut(s) 826
GlaI GCGC 1 cut(s) 826
GluI GCNGC 8 cut(s) 93, 290, 1034, 1166, 1202, 1205, 1208, 1211
GsaI CCCAGC 2 cut(s) 618, 1115
GsuI CTGGAG 2 cut(s) 725, 1010
HaeIII GGCC 5 cut(s) 402, 675, 972, 1131, 1136
HapII CCGG 2 cut(s) 672, 1107
HhaI GCGC 1 cut(s) 827
Hin1II CATG 2 cut(s) 1078, 1180
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 2 cut(s) 1039, 1103
HpaII CCGG 2 cut(s) 672, 1107
HphI GGTGA 4 cut(s) 363, 372, 730, 1331
Hpy166II GTNNAC 3 cut(s) 922, 1011, 1234
Hpy188I TCNGA 5 cut(s) 29, 126, 478, 655, 785
Hpy188III TCNNGA 4 cut(s) 196, 394, 406, 1096
Hpy8I GTNNAC 3 cut(s) 922, 1011, 1234
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 3 cut(s) 1222, 1291, 1307
HpyCH4III ACNGT 2 cut(s) 138, 375
HpyCH4IV ACGT 1 cut(s) 1050
HpyCH4V TGCA 3 cut(s) 1036, 1168, 1234
HpyF10VI GCNNNNNNNGC 3 cut(s) 295, 1207, 1210
HpyF3I CTNAG 2 cut(s) 186, 475
HpySE526I ACGT 1 cut(s) 1050
Hsp92II CATG 2 cut(s) 1078, 1180
HspAI GCGC 1 cut(s) 825
Kzo9I GATC 5 cut(s) 192, 478, 621, 693, 1083
LmnI GCTCC 4 cut(s) 10, 87, 852, 906
Lsp1109I GCAGC 5 cut(s) 276, 1020, 1152, 1188, 1219
LweI GCATC 1 cut(s) 882
MabI ACCWGGT 1 cut(s) 764
MaeI CTAG 3 cut(s) 44, 482, 851
MaeII ACGT 1 cut(s) 1050
MaeIII GTNAC 2 cut(s) 964, 1253
MalI GATC 5 cut(s) 194, 480, 623, 695, 1085
MboI GATC 5 cut(s) 192, 478, 621, 693, 1083
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 5 cut(s) 131, 981, 1056, 1118, 1236
MluCI AATT 5 cut(s) 120, 164, 415, 677, 1222
MlyI GAGTC 2 cut(s) 1033, 1097
MmeI TCCRAC 5 cut(s) 141, 634, 678, 706, 763
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 1181
MspA1I CMGCKG 1 cut(s) 618
MspI CCGG 2 cut(s) 672, 1107
MspR9I CCNGG 7 cut(s) 672, 704, 766, 1028, 1088, 1108, 1133
Mva1269I GAATGC 1 cut(s) 827
MvaI CCWGG 5 cut(s) 704, 766, 1028, 1088, 1133
MvnI CGCG 1 cut(s) 1157
MwoI GCNNNNNNNGC 3 cut(s) 295, 1207, 1210
NciI CCSGG 2 cut(s) 672, 1108
NdeII GATC 5 cut(s) 192, 478, 621, 693, 1083
NlaIII CATG 2 cut(s) 1078, 1180
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 1023
NmuCI GTSAC 1 cut(s) 964
NsbI TGCGCA 1 cut(s) 826
NspI RCATGY 1 cut(s) 1180
PaeR7I CTCGAG 1 cut(s) 974
PciI ACATGT 1 cut(s) 1176
PctI GAATGC 1 cut(s) 827
PflFI GACNNNGTC 1 cut(s) 647
PkrI GCNGC 8 cut(s) 94, 291, 1035, 1167, 1203, 1206, 1209, 1212
PleI GAGTC 2 cut(s) 1033, 1097
PpsI GAGTC 2 cut(s) 1033, 1097
Ppu21I YACGTR 1 cut(s) 1051
PscI ACATGT 1 cut(s) 1176
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 5 cut(s) 702, 764, 1026, 1086, 1131
PspFI CCCAGC 2 cut(s) 614, 1111
PspGI CCWGG 5 cut(s) 702, 764, 1026, 1086, 1131
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 1129, 1135
PspXI VCTCGAGB 1 cut(s) 974
PstI CTGCAG 1 cut(s) 1038
PstNI CAGNNNCTG 2 cut(s) 691, 1033
PsuI RGATCY 1 cut(s) 478
PsyI GACNNNGTC 1 cut(s) 647
PvuII CAGCTG 1 cut(s) 618
RsaI GTAC 2 cut(s) 36, 1079
RsaNI GTAC 2 cut(s) 35, 1078
RseI CAYNNNNRTG 1 cut(s) 1181
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 8 cut(s) 93, 290, 1034, 1166, 1202, 1205, 1208, 1211
Sau3AI GATC 5 cut(s) 192, 478, 621, 693, 1083
Sau96I GGNCC 2 cut(s) 1129, 1135
SchI GAGTC 2 cut(s) 1033, 1097
ScrFI CCNGG 7 cut(s) 672, 704, 766, 1028, 1088, 1108, 1133
SduI GDGCHC 5 cut(s) 131, 981, 1056, 1118, 1236
SexAI ACCWGGT 1 cut(s) 764
SfaNI GCATC 1 cut(s) 882
SfcI CTRYAG 1 cut(s) 1034
Sfr274I CTCGAG 1 cut(s) 974
SlaI CTCGAG 1 cut(s) 974
SmiMI CAYNNNNRTG 1 cut(s) 1181
SmlI CTYRAG 4 cut(s) 293, 404, 974, 983
SmoI CTYRAG 4 cut(s) 293, 404, 974, 983
Sse9I AATT 5 cut(s) 120, 164, 415, 677, 1222
SsiI CCGC 7 cut(s) 93, 871, 1157, 1196, 1204, 1211, 1283
SspMI CTAG 3 cut(s) 44, 482, 851
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 7 cut(s) 670, 702, 764, 1026, 1086, 1106, 1131
StyI CCWWGG 1 cut(s) 960
TaaI ACNGT 2 cut(s) 138, 375
TaiI ACGT 1 cut(s) 1053
TaqI TCGA 5 cut(s) 118, 624, 975, 1097, 1118
TaqII GACCGA 1 cut(s) 1115
TasI AATT 5 cut(s) 120, 164, 415, 677, 1222
TatI WGTACW 1 cut(s) 1077
TauI GCSGC 3 cut(s) 95, 1207, 1213
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 2 cut(s) 696, 973
TseFI GTSAC 1 cut(s) 964
TseI GCWGC 5 cut(s) 289, 1033, 1165, 1201, 1207
Tsp45I GTSAC 1 cut(s) 964
TspDTI ATGAA 3 cut(s) 186, 252, 901
TspRI CASTG 2 cut(s) 696, 973
Tth111I GACNNNGTC 1 cut(s) 647
VneI GTGCAC 1 cut(s) 1232
XapI RAATTY 1 cut(s) 120
XceI RCATGY 1 cut(s) 1180
XhoI CTCGAG 1 cut(s) 974
XmiI GTMKAC 1 cut(s) 921
XspI CTAG 3 cut(s) 44, 482, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.