pycom01g05640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014549
Physical Location & Seq
Forward (+)
5492332 .. 5493750
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05640.2

Sequence Viewer

Length: 1296 bp
ATGGCTCCACTCATGTACTTGAGTCTCGGAAAGGTACTCCTGCTTTCCTCTGCTGTTTTCTTCCTCTTTCCTACTCTCCTCCTTTCTGCCCGCTCCGAGTCAGTTGAGACCTCACAGTTTGTTGCGAGTACAAGGAAGATCTTGTTAGAGTTAGAAATCGACCCAGACCAAGACCTGCCCAAAAAGATCAATGATCAAACCAACCTTGTCATCCTCAAAAAACAAGACCAAATTGATCAAACCAACCTCTTCATCCTCAAAAAACCAGACCAAATTGATCAAACCAACCTTGTCATCCTCAAAAAACCAGACCAAACTCCTCAAACCGATTTGAAATCCACCAAGAATGGCGAGTTGAAGAAGCTCAATCCCACATCAAAACCCTCAAATTCCACCACACCCACATCAAATTCCTCGAAGAAAACCTCAGATCTAACTAAAATAAGCTTACCCAAGAACCAAACCACCAAACCCACCACCCCGAAACAATCCCAAAACCTCGTCGACAAGAAAAAAACCACCGACGCTAAAAAACCCGCCCAACAGAAACCCAAAAAACCAGTCGAACCCAGCTGGATCGACCAAGAAGACGACACCGATTTCGTCTCCGACTTCACCGACTTACCCGGCAAATTCCAGCAAACCCTAATCCCAGATTTGGAGAGAATTCGGACCACCTCGAAGATTTACCTCACCGAAGCCAACAAACAAATGACGAACCAGTTCAAGCCCGTTGTCGGTAAGAAGTACGCCGCCACCATCGCCTCCACCGTGTCGTGCGTCTTCCTGATTATCCCTCTGCTGTTAGTCTCTCTCCTCTTCAACCGCATCAAAGCCTACTTCTCTCTCCAAAAGCTCTTGATCTTCATTCAAGTCTACCTCTCCATCTACTTCTCCATCCTCTGCCTCTCAGCTCTCGTCACCGGCCTCGAACCTCTCAAGTTTTTCTACGCCACTTCGGACTCCACCTACGTCTGCCTGCAGGTGCTGCAGACGCTCGGCTACGTTCTGTACCTCCTGCTGCTGCTCATGTACTTGATTCTGGTTTTCTCGACCGATTCCGGGTTGGGCTCGAAGGTGTTGGGCTTTGCCCAGACCTTCGTCGGCTTCTCCGTTGGGCTCCATTACTACATGTCGGTGTTTCACAGAGCTGTGCTGAGGCAGCCGCCCAAGACCAACTGGAAGGTGCATGGACTTTACGCCACGTGTTTCCTTGTGATTTGTCTGTTTGCCACCGCCGACAGAAGGAAGAAGGCTTACTTGGAAGAGGGCGGTGAAGAGGGCAAGAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

432

Amino Acids

48.5

Weight (kDa)

9.41

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39840
malus_domestica MD01G1028100.v1.1 MD15G1325300.v1.1
prunus_persica Prupe.6G184700_v2.0.a1
pyrus_communis pycom01g05640
rosa_chinensis RchiOBHm_Chr2g0114541
rosa_laevigata RLG00000018113
rosa_multiflora Rmu_sc0000370.1_g000001 Rmu_sc0000371.1_g000011 Rmu_sc0027184.1_g000005
rosa_roxburghii Rroxscaffold_2G00130280
rosa_rugosa Rorug02G0190700
rosa_samantha Rh2AG248600 Rh2BG258200 Rh2CG252000 Rh2DG256700
rosa_wichuraiana Rw2G019330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 973
Acc36I ACCTGC 2 cut(s) 183, 973
AccBSI CCGCTC 1 cut(s) 93
AccI GTMKAC 2 cut(s) 504, 876
AciI CCGC 7 cut(s) 91, 537, 753, 826, 1166, 1236, 1272
AclWI GGATC 1 cut(s) 584
AcsI RAATTY 4 cut(s) 388, 409, 632, 666
AcvI CACGTG 1 cut(s) 1206
AfaI GTAC 6 cut(s) 17, 36, 130, 749, 1013, 1034
AfiI CCNNNNNNNGG 4 cut(s) 658, 737, 1062, 1245
AflIII ACRYGT 2 cut(s) 1131, 1205
AgsI TTSAA 5 cut(s) 334, 358, 727, 823, 872
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
AluBI AGCT 6 cut(s) 364, 447, 573, 856, 914, 1151
AluI AGCT 6 cut(s) 364, 447, 573, 856, 914, 1151
Alw26I GTCTC 4 cut(s) 29, 101, 610, 814
AlwI GGATC 1 cut(s) 584
AlwNI CAGNNNCTG 1 cut(s) 988
AoxI GGCC 1 cut(s) 925
ApeKI GCWGC 4 cut(s) 988, 1021, 1024, 1162
ApoI RAATTY 4 cut(s) 388, 409, 632, 666
ArsI GACNNNNNNTTYG 4 cut(s) 546, 578, 584, 616
Asp700I GAANNNNTTC 1 cut(s) 722
AspS9I GGNCC 1 cut(s) 672
AsuC2I CCSGG 2 cut(s) 627, 1063
AsuHPI GGTGA 4 cut(s) 607, 685, 913, 1286
AvaII GGWCC 1 cut(s) 672
BanII GRGCYC 2 cut(s) 1073, 1122
BarI GAAGNNNNNNTAC 2 cut(s) 1241, 1273
BbrPI CACGTG 1 cut(s) 1206
BbsI GAAGAC 2 cut(s) 594, 775
BbvCI CCTCAGC 1 cut(s) 1157
BbvI GCAGC 4 cut(s) 975, 1008, 1011, 1174
BccI CCATC 3 cut(s) 767, 893, 905
BclI TGATCA 3 cut(s) 193, 235, 277
BcnI CCSGG 2 cut(s) 627, 1063
BcoDI GTCTC 4 cut(s) 29, 101, 610, 814
BfmI CTRYAG 2 cut(s) 980, 989
BfuAI ACCTGC 2 cut(s) 183, 973
BglII AGATCT 2 cut(s) 138, 430
BisI GCNGC 6 cut(s) 753, 989, 1022, 1025, 1163, 1166
BlsI GCNGC 6 cut(s) 754, 990, 1023, 1026, 1164, 1167
Bme1390I CCNGG 2 cut(s) 627, 1063
Bme18I GGWCC 1 cut(s) 672
BmgT120I GGNCC 1 cut(s) 672
BmiI GGNNCC 2 cut(s) 6, 1121
BmrFI CCNGG 2 cut(s) 627, 1063
BmsI GCATC 1 cut(s) 837
BoxI GACNNNNGTC 1 cut(s) 1100
BpiI GAAGAC 2 cut(s) 594, 775
Bpu10I CCTNAGC 1 cut(s) 1157
BpuEI CTTGAG 2 cut(s) 40, 923
BpuMI CCSGG 2 cut(s) 627, 1063
BsaAI YACGTR 1 cut(s) 1206
BsaI GGTCTC 1 cut(s) 101
Bsc4I CCNNNNNNNGG 4 cut(s) 658, 737, 1062, 1245
Bse118I RCCGGY 1 cut(s) 923
Bse1I ACTGG 3 cut(s) 560, 721, 1184
BseGI GGATG 4 cut(s) 210, 252, 294, 897
BseLI CCNNNNNNNGG 4 cut(s) 658, 737, 1062, 1245
BseMII CTCAG 3 cut(s) 441, 924, 1148
BseNI ACTGG 3 cut(s) 560, 721, 1184
BseRI GAGGAG 3 cut(s) 68, 309, 806
BseXI GCAGC 4 cut(s) 975, 1008, 1011, 1174
BseYI CCCAGC 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 1056
BshFI GGCC 1 cut(s) 927
BsiEI CGRYCG 1 cut(s) 1056
BsiSI CCGG 3 cut(s) 627, 924, 1062
BslI CCNNNNNNNGG 4 cut(s) 658, 737, 1062, 1245
BsmAI GTCTC 4 cut(s) 29, 101, 610, 814
BsmBI CGTCTC 1 cut(s) 610
BsnI GGCC 1 cut(s) 927
Bso31I GGTCTC 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 1073, 1122
Bsp143I GATC 8 cut(s) 138, 186, 193, 235, 277, 430, 576, 861
BspACI CCGC 7 cut(s) 91, 537, 753, 826, 1166, 1236, 1272
BspANI GGCC 1 cut(s) 927
BspCNI CTCAG 3 cut(s) 440, 923, 1149
BspLI GGNNCC 2 cut(s) 6, 1121
BspMAI CTGCAG 2 cut(s) 984, 993
BspMI ACCTGC 2 cut(s) 183, 973
BspPI GGATC 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 923
BsrI ACTGG 3 cut(s) 560, 721, 1184
BssAI RCCGGY 1 cut(s) 923
BssMI GATC 8 cut(s) 138, 186, 193, 235, 277, 430, 576, 861
Bst4CI ACNGT 2 cut(s) 117, 772
Bst6I CTCTTC 5 cut(s) 254, 824, 1260, 1272, 1283
BstAPI GCANNNNNTGC 1 cut(s) 988
BstBAI YACGTR 1 cut(s) 1206
BstC8I GCNNGC 2 cut(s) 91, 980
BstDEI CTNAG 3 cut(s) 427, 910, 1157
BstF5I GGATG 4 cut(s) 210, 252, 294, 897
BstKTI GATC 8 cut(s) 141, 189, 196, 238, 280, 433, 579, 864
BstMAI GTCTC 4 cut(s) 29, 101, 610, 814
BstMBI GATC 8 cut(s) 138, 186, 193, 235, 277, 430, 576, 861
BstMCI CGRYCG 1 cut(s) 1056
BstMWI GCNNNNNNNGC 4 cut(s) 761, 988, 994, 1162
BstNSI RCATGY 1 cut(s) 1135
BstPAI GACNNNNGTC 1 cut(s) 1100
BstSCI CCNGG 2 cut(s) 625, 1061
BstSFI CTRYAG 2 cut(s) 980, 989
BstV1I GCAGC 4 cut(s) 975, 1008, 1011, 1174
BstV2I GAAGAC 2 cut(s) 594, 775
BstX2I RGATCY 2 cut(s) 138, 430
BstYI RGATCY 2 cut(s) 138, 430
BsuRI GGCC 1 cut(s) 927
BtgZI GCGATG 1 cut(s) 745
BtsCI GGATG 4 cut(s) 210, 252, 294, 897
BveI ACCTGC 2 cut(s) 183, 973
Cac8I GCNNGC 2 cut(s) 91, 980
CaiI CAGNNNCTG 1 cut(s) 988
Cfr10I RCCGGY 1 cut(s) 923
Cfr13I GGNCC 1 cut(s) 672
CseI GACGC 3 cut(s) 533, 769, 1003
Csp6I GTAC 6 cut(s) 16, 35, 129, 748, 1012, 1033
CviAII CATG 4 cut(s) 13, 1030, 1132, 1190
CviQI GTAC 6 cut(s) 16, 35, 129, 748, 1012, 1033
DdeI CTNAG 3 cut(s) 427, 910, 1157
DpnI GATC 8 cut(s) 140, 188, 195, 237, 279, 432, 578, 863
DpnII GATC 8 cut(s) 138, 186, 193, 235, 277, 430, 576, 861
Eam1104I CTCTTC 5 cut(s) 254, 824, 1260, 1272, 1283
EarI CTCTTC 5 cut(s) 254, 824, 1260, 1272, 1283
Eco24I GRGCYC 2 cut(s) 1073, 1122
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 672
Eco72I CACGTG 1 cut(s) 1206
EcoRI GAATTC 1 cut(s) 666
EcoT38I GRGCYC 2 cut(s) 1073, 1122
Esp3I CGTCTC 1 cut(s) 610
FaeI CATG 4 cut(s) 16, 1033, 1135, 1193
FaiI YATR 4 cut(s) 14, 1031, 1133, 1191
FalI AAGNNNNNCTT 2 cut(s) 1244, 1276
FatI CATG 4 cut(s) 12, 1029, 1131, 1189
FauI CCCGC 2 cut(s) 98, 544
FbaI TGATCA 3 cut(s) 193, 235, 277
FblI GTMKAC 2 cut(s) 504, 876
Fnu4HI GCNGC 6 cut(s) 753, 989, 1022, 1025, 1163, 1166
FokI GGATG 4 cut(s) 197, 239, 281, 884
FriOI GRGCYC 2 cut(s) 1073, 1122
Fsp4HI GCNGC 6 cut(s) 753, 989, 1022, 1025, 1163, 1166
GluI GCNGC 6 cut(s) 753, 989, 1022, 1025, 1163, 1166
GsaI CCCAGC 1 cut(s) 573
HaeIII GGCC 1 cut(s) 927
HapII CCGG 3 cut(s) 627, 924, 1062
HgaI GACGC 3 cut(s) 533, 769, 1003
Hin1II CATG 4 cut(s) 16, 1033, 1135, 1193
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 445
HinfI GANTC 5 cut(s) 22, 98, 962, 1039, 1058
HpaII CCGG 3 cut(s) 627, 924, 1062
HphI GGTGA 4 cut(s) 607, 685, 913, 1286
Hpy166II GTNNAC 2 cut(s) 505, 877
Hpy188I TCNGA 6 cut(s) 29, 97, 430, 610, 672, 961
Hpy188III TCNNGA 3 cut(s) 787, 859, 1051
Hpy8I GTNNAC 2 cut(s) 505, 877
Hpy99I CGWCG 3 cut(s) 506, 527, 1106
HpyAV CCTTC 5 cut(s) 1069, 1108, 1177, 1239, 1246
HpyCH4III ACNGT 2 cut(s) 117, 772
HpyCH4IV ACGT 3 cut(s) 972, 1005, 1205
HpyCH4V TGCA 3 cut(s) 982, 991, 1189
HpyF10VI GCNNNNNNNGC 4 cut(s) 761, 988, 994, 1162
HpyF3I CTNAG 3 cut(s) 427, 910, 1157
HpySE526I ACGT 3 cut(s) 972, 1005, 1205
Hsp92II CATG 4 cut(s) 16, 1033, 1135, 1193
Ksp22I TGATCA 3 cut(s) 193, 235, 277
Kzo9I GATC 8 cut(s) 138, 186, 193, 235, 277, 430, 576, 861
LmnI GCTCC 3 cut(s) 10, 98, 1125
Lsp1109I GCAGC 4 cut(s) 975, 1008, 1011, 1174
LweI GCATC 1 cut(s) 837
MaeII ACGT 3 cut(s) 972, 1005, 1205
MaeIII GTNAC 1 cut(s) 919
MalI GATC 8 cut(s) 140, 188, 195, 237, 279, 432, 578, 863
MbiI CCGCTC 1 cut(s) 93
MboI GATC 8 cut(s) 138, 186, 193, 235, 277, 430, 576, 861
MflI RGATCY 2 cut(s) 138, 430
MhlI GDGCHC 2 cut(s) 1073, 1122
MluCI AATT 6 cut(s) 231, 273, 388, 409, 632, 666
MlyI GAGTC 3 cut(s) 31, 107, 956
MmeI TCCRAC 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 1 cut(s) 1294
MslI CAYNNNNRTG 1 cut(s) 1136
MspA1I CMGCKG 1 cut(s) 573
MspI CCGG 3 cut(s) 627, 924, 1062
MspR9I CCNGG 2 cut(s) 627, 1063
MwoI GCNNNNNNNGC 4 cut(s) 761, 988, 994, 1162
NciI CCSGG 2 cut(s) 627, 1063
NdeII GATC 8 cut(s) 138, 186, 193, 235, 277, 430, 576, 861
NlaIII CATG 4 cut(s) 16, 1033, 1135, 1193
NlaIV GGNNCC 2 cut(s) 6, 1121
NmeAIII GCCGAG 1 cut(s) 978
NmuCI GTSAC 1 cut(s) 919
NspI RCATGY 1 cut(s) 1135
PaqCI CACCTGC 1 cut(s) 973
PciI ACATGT 1 cut(s) 1131
PcsI WCGNNNNNNNCGW 2 cut(s) 768, 1110
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 2 cut(s) 1039, 1058
PkrI GCNGC 6 cut(s) 754, 990, 1023, 1026, 1164, 1167
PleI GAGTC 3 cut(s) 30, 106, 956
PmaCI CACGTG 1 cut(s) 1206
PmlI CACGTG 1 cut(s) 1206
PpsI GAGTC 3 cut(s) 30, 106, 956
Ppu21I YACGTR 1 cut(s) 1206
PscI ACATGT 1 cut(s) 1131
PshAI GACNNNNGTC 1 cut(s) 1100
PspCI CACGTG 1 cut(s) 1206
PspFI CCCAGC 1 cut(s) 569
PspN4I GGNNCC 2 cut(s) 6, 1121
PspPI GGNCC 1 cut(s) 672
PstI CTGCAG 2 cut(s) 984, 993
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 2 cut(s) 138, 430
PvuII CAGCTG 1 cut(s) 573
RsaI GTAC 6 cut(s) 17, 36, 130, 749, 1013, 1034
RsaNI GTAC 6 cut(s) 16, 35, 129, 748, 1012, 1033
RseI CAYNNNNRTG 1 cut(s) 1136
SalI GTCGAC 1 cut(s) 503
SaqAI TTAA 1 cut(s) 1294
SatI GCNGC 6 cut(s) 753, 989, 1022, 1025, 1163, 1166
Sau3AI GATC 8 cut(s) 138, 186, 193, 235, 277, 430, 576, 861
Sau96I GGNCC 1 cut(s) 672
SbfI CCTGCAGG 1 cut(s) 984
SchI GAGTC 3 cut(s) 31, 107, 956
ScrFI CCNGG 2 cut(s) 627, 1063
SdaI CCTGCAGG 1 cut(s) 984
SduI GDGCHC 2 cut(s) 1073, 1122
SfaNI GCATC 1 cut(s) 837
SfcI CTRYAG 2 cut(s) 980, 989
SinI GGWCC 1 cut(s) 672
SmiMI CAYNNNNRTG 1 cut(s) 1136
SmlI CTYRAG 2 cut(s) 19, 938
SmoI CTYRAG 2 cut(s) 19, 938
Sse8387I CCTGCAGG 1 cut(s) 984
Sse9I AATT 6 cut(s) 231, 273, 388, 409, 632, 666
SsiI CCGC 7 cut(s) 91, 537, 753, 826, 1166, 1236, 1272
StyD4I CCNGG 2 cut(s) 625, 1061
TaaI ACNGT 2 cut(s) 117, 772
TaiI ACGT 3 cut(s) 975, 1008, 1208
TaqI TCGA 9 cut(s) 159, 416, 504, 564, 579, 680, 930, 1052, 1073
TaqII GACCGA 1 cut(s) 1070
TasI AATT 6 cut(s) 231, 273, 388, 409, 632, 666
TatI WGTACW 3 cut(s) 15, 128, 1032
TauI GCSGC 2 cut(s) 755, 1168
TfiI GAWTC 2 cut(s) 1039, 1058
Tru1I TTAA 1 cut(s) 1294
Tru9I TTAA 1 cut(s) 1294
TseFI GTSAC 1 cut(s) 919
TseI GCWGC 4 cut(s) 988, 1021, 1024, 1162
Tsp45I GTSAC 1 cut(s) 919
TspDTI ATGAA 2 cut(s) 241, 856
TspGWI ACGGA 1 cut(s) 1102
VpaK11BI GGWCC 1 cut(s) 672
XapI RAATTY 4 cut(s) 388, 409, 632, 666
XceI RCATGY 1 cut(s) 1135
XmiI GTMKAC 2 cut(s) 504, 876
XmnI GAANNNNTTC 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.