Rw2G019330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014549
Physical Location & Seq
Reverse (-)
25307166 .. 25308505
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G019330.1

Sequence Viewer

Length: 1158 bp
ATGGCTCCACTCATGTACTTCAAGTTCAGAAAGGTACTTTTGCTAGTTTCTCTTGTTGTGTTCCTCTTTTCTTCTACTCTGCTCCTTTGTGCCGCAGAAGAAGATGAGAAACTGCCTCGAATTCAGAGCTCATCACAGTTTTTGGCTTCAAGGAAAATGTTGGAATTAGATGAAGAAGTCCAGCCTAAGAAGATCAAGACCAAAACCACCACCTCAACTCCCCAATCCTCCAAAAACCAAACCAAGCTTATCAAACCCACTTCATCATCCTCCAAAAACCAAACCAAGCTGCTCAAGCCACTTAAACCCAACCTATCATCCAAGAACCAGACCAAGCTCATCAAACCAAGCACCAATCCAACCAAGTCATCATCGTCATCACCACCCAAAAACCCCCACCCACAAAAACCCACCAAGAAACCAATCCAACCCAGCTGGATCGAACAAGAAGACGACACCGACTTCGTCTCCGACTTCACAGACTTACCCGGCCAATTCCAACAGACTCTGATCCCAGACCTGGAGAAAATCTCAACCACATCAAAAATCTACCTCACCCAAGCCAACAAGCAAATGACCACCAGGTTCAAACCACTCGTCGGAAACAAATACGCCCCCACCATCGCTTCCATAGTCTCCTGCGCATTCCTACTTATCCCATTGCTCCTAGTCTCTCTCCTCTTCAACAGAATCAAAGCCTACTTTTCCCTCCAAAAGCTCCTCATCTTCATCCAAGTCTACCTCTCCATCTACTTCTCAATCCTCTGCCTCTCTTCCTTGGTCACTGGCCTCGAGCCCCTCAAGTTTTTCTACGCTACTTCGCAGTCCACCTACATCTGCCTCCAGGTGCTGCAGACTCTCGGCTACGTGCTCTATCTCCTCCTCCTCATCATGTACTTGATCCTGGTTTTCTCGACCGACTCCGGGCTGGGCTCGAAGATGTTGGGCCTGGCCCAGACTTTTCTCGGGTTCGCGGTCGGGCTGCATTACTACATGTCGGTGTTTCACAGGGCGGTGCTGCGGCAGCCGCCGAAGACTAATTGGAAGGTGCACGGGATATACGCCGTGTGTTACCTTGTGATTTGTCTGTTTGCCACGGCGGAGAGGAGGAAGAAGGCTTACTTGGAAGAAGGTGGTGAAGAGGGCAAGAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

385

Amino Acids

43.45

Weight (kDa)

9.66

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39840
malus_domestica MD01G1028100.v1.1 MD15G1325300.v1.1
prunus_persica Prupe.6G184700_v2.0.a1
pyrus_communis pycom01g05640
rosa_chinensis RchiOBHm_Chr2g0114541
rosa_laevigata RLG00000018113
rosa_multiflora Rmu_sc0000370.1_g000001 Rmu_sc0000371.1_g000011 Rmu_sc0027184.1_g000005
rosa_roxburghii Rroxscaffold_2G00130280
rosa_rugosa Rorug02G0190700
rosa_samantha Rh2AG248600 Rh2BG258200 Rh2CG252000 Rh2DG256700
rosa_wichuraiana Rw2G019330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 643
AccI GTMKAC 1 cut(s) 738
AccII CGCG 1 cut(s) 974
AciI CCGC 6 cut(s) 93, 974, 1013, 1021, 1028, 1100
AclWI GGATC 3 cut(s) 446, 505, 895
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 1 cut(s) 120
AfaI GTAC 3 cut(s) 17, 36, 896
AfiI CCNNNNNNNGG 3 cut(s) 520, 599, 924
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 4 cut(s) 22, 150, 589, 685
AjnI CCWGG 5 cut(s) 519, 581, 843, 903, 948
AjuI GAANNNNNNNTTGG 2 cut(s) 1106, 1138
AluBI AGCT 6 cut(s) 129, 247, 289, 337, 435, 718
AluI AGCT 6 cut(s) 129, 247, 289, 337, 435, 718
Alw21I GWGCWC 3 cut(s) 131, 873, 1053
Alw26I GTCTC 3 cut(s) 472, 640, 676
Alw44I GTGCAC 1 cut(s) 1049
AlwI GGATC 3 cut(s) 446, 505, 895
AlwNI CAGNNNCTG 2 cut(s) 508, 850
Ama87I CYCGRG 2 cut(s) 791, 965
AoxI GGCC 4 cut(s) 490, 787, 946, 951
ApaLI GTGCAC 1 cut(s) 1049
ApeKI GCWGC 5 cut(s) 289, 850, 982, 1018, 1024
ApoI RAATTY 1 cut(s) 120
ArsI GACNNNNNNTTYG 4 cut(s) 446, 478, 582, 614
AspLEI GCGC 1 cut(s) 644
AspS9I GGNCC 2 cut(s) 946, 952
AsuC2I CCSGG 2 cut(s) 489, 925
AsuHPI GGTGA 3 cut(s) 372, 547, 1148
AvaI CYCGRG 2 cut(s) 791, 965
BaeGI GKGCMC 1 cut(s) 1053
BanII GRGCYC 3 cut(s) 131, 798, 935
BarI GAAGNNNNNNTAC 2 cut(s) 1103, 1135
BbsI GAAGAC 2 cut(s) 456, 1040
Bbv12I GWGCWC 3 cut(s) 131, 873, 1053
BbvI GCAGC 5 cut(s) 276, 837, 969, 1005, 1036
BccI CCATC 2 cut(s) 629, 755
BceAI ACGGC 2 cut(s) 1049, 1113
BciT130I CCWGG 5 cut(s) 521, 583, 845, 905, 950
BcnI CCSGG 2 cut(s) 489, 925
BcoDI GTCTC 3 cut(s) 472, 640, 676
BfaI CTAG 2 cut(s) 44, 668
BfmI CTRYAG 1 cut(s) 851
BisI GCNGC 8 cut(s) 93, 290, 851, 983, 1019, 1022, 1025, 1028
BlsI GCNGC 8 cut(s) 94, 291, 852, 984, 1020, 1023, 1026, 1029
Bme1390I CCNGG 7 cut(s) 489, 521, 583, 845, 905, 925, 950
BmeT110I CYCGRG 2 cut(s) 791, 965
BmgT120I GGNCC 2 cut(s) 946, 952
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 7 cut(s) 489, 521, 583, 845, 905, 925, 950
BpiI GAAGAC 2 cut(s) 456, 1040
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpmI CTGGAG 2 cut(s) 542, 827
BpuEI CTTGAG 2 cut(s) 278, 785
BpuMI CCSGG 2 cut(s) 489, 925
BsaAI YACGTR 1 cut(s) 868
BsaJI CCNNGG 2 cut(s) 777, 1095
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 452, 482
Bsc4I CCNNNNNNNGG 3 cut(s) 520, 599, 924
Bse1I ACTGG 1 cut(s) 790
Bse3DI GCAATG 1 cut(s) 659
BseBI CCWGG 5 cut(s) 521, 583, 845, 905, 950
BseDI CCNNGG 2 cut(s) 777, 1095
BseGI GGATG 3 cut(s) 266, 317, 729
BseLI CCNNNNNNNGG 3 cut(s) 520, 599, 924
BseMI GCAATG 1 cut(s) 659
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 6 cut(s) 668, 710, 869, 872, 875, 1120
BseSI GKGCMC 1 cut(s) 1053
BseXI GCAGC 5 cut(s) 276, 837, 969, 1005, 1036
BseYI CCCAGC 2 cut(s) 431, 928
Bsh1236I CGCG 1 cut(s) 974
Bsh1285I CGRYCG 2 cut(s) 918, 978
BshFI GGCC 4 cut(s) 492, 789, 948, 953
BsiEI CGRYCG 2 cut(s) 918, 978
BsiHKAI GWGCWC 3 cut(s) 131, 873, 1053
BsiHKCI CYCGRG 2 cut(s) 791, 965
BsiSI CCGG 2 cut(s) 489, 924
BslI CCNNNNNNNGG 3 cut(s) 520, 599, 924
BsmAI GTCTC 3 cut(s) 472, 640, 676
BsmBI CGTCTC 1 cut(s) 472
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 4 cut(s) 492, 789, 948, 953
BsoBI CYCGRG 2 cut(s) 791, 965
Bsp1286I GDGCHC 5 cut(s) 131, 798, 873, 935, 1053
Bsp143I GATC 4 cut(s) 192, 438, 510, 900
BspACI CCGC 6 cut(s) 93, 974, 1013, 1021, 1028, 1100
BspANI GGCC 4 cut(s) 492, 789, 948, 953
BspFNI CGCG 1 cut(s) 974
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 855
BspPI GGATC 3 cut(s) 446, 505, 895
BsrDI GCAATG 1 cut(s) 659
BsrI ACTGG 1 cut(s) 790
BssECI CCNNGG 2 cut(s) 777, 1095
BssMI GATC 4 cut(s) 192, 438, 510, 900
BssT1I CCWWGG 1 cut(s) 777
Bst2UI CCWGG 5 cut(s) 521, 583, 845, 905, 950
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 3 cut(s) 686, 778, 1134
BstBAI YACGTR 1 cut(s) 868
BstDEI CTNAG 1 cut(s) 186
BstDSI CCRYGG 1 cut(s) 1095
BstF5I GGATG 3 cut(s) 266, 317, 729
BstFNI CGCG 1 cut(s) 974
BstHHI GCGC 1 cut(s) 644
BstKTI GATC 4 cut(s) 195, 441, 513, 903
BstMAI GTCTC 3 cut(s) 472, 640, 676
BstMBI GATC 4 cut(s) 192, 438, 510, 900
BstMCI CGRYCG 2 cut(s) 918, 978
BstMWI GCNNNNNNNGC 3 cut(s) 295, 1024, 1027
BstNI CCWGG 5 cut(s) 521, 583, 845, 905, 950
BstNSI RCATGY 1 cut(s) 997
BstSCI CCNGG 7 cut(s) 487, 519, 581, 843, 903, 923, 948
BstSFI CTRYAG 1 cut(s) 851
BstSLI GKGCMC 1 cut(s) 1053
BstUI CGCG 1 cut(s) 974
BstV1I GCAGC 5 cut(s) 276, 837, 969, 1005, 1036
BstV2I GAAGAC 2 cut(s) 456, 1040
BsuRI GGCC 4 cut(s) 492, 789, 948, 953
BtgI CCRYGG 1 cut(s) 1095
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 3 cut(s) 266, 317, 729
BtsIMutI CAGTG 1 cut(s) 783
CaiI CAGNNNCTG 2 cut(s) 508, 850
CfoI GCGC 1 cut(s) 644
Cfr13I GGNCC 2 cut(s) 946, 952
CsiI ACCWGGT 1 cut(s) 581
Csp6I GTAC 3 cut(s) 16, 35, 895
CviAII CATG 3 cut(s) 13, 892, 994
CviQI GTAC 3 cut(s) 16, 35, 895
DdeI CTNAG 1 cut(s) 186
DpnI GATC 4 cut(s) 194, 440, 512, 902
DpnII GATC 4 cut(s) 192, 438, 510, 900
EaeI YGGCCR 1 cut(s) 490
Eam1104I CTCTTC 3 cut(s) 686, 778, 1134
EarI CTCTTC 3 cut(s) 686, 778, 1134
EciI GGCGGA 1 cut(s) 1115
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 777
Eco24I GRGCYC 3 cut(s) 131, 798, 935
Eco53kI GAGCTC 1 cut(s) 129
Eco88I CYCGRG 2 cut(s) 791, 965
EcoICRI GAGCTC 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 5 cut(s) 519, 581, 843, 903, 948
EcoT14I CCWWGG 1 cut(s) 777
EcoT38I GRGCYC 3 cut(s) 131, 798, 935
ErhI CCWWGG 1 cut(s) 777
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 3 cut(s) 16, 895, 997
FaiI YATR 5 cut(s) 14, 632, 893, 995, 1060
FalI AAGNNNNNCTT 2 cut(s) 1106, 1138
FatI CATG 3 cut(s) 12, 891, 993
FblI GTMKAC 1 cut(s) 738
Fnu4HI GCNGC 8 cut(s) 93, 290, 851, 983, 1019, 1022, 1025, 1028
FokI GGATG 3 cut(s) 253, 304, 716
FriOI GRGCYC 3 cut(s) 131, 798, 935
Fsp4HI GCNGC 8 cut(s) 93, 290, 851, 983, 1019, 1022, 1025, 1028
FspBI CTAG 2 cut(s) 44, 668
FspI TGCGCA 1 cut(s) 643
GlaI GCGC 1 cut(s) 643
GluI GCNGC 8 cut(s) 93, 290, 851, 983, 1019, 1022, 1025, 1028
GsaI CCCAGC 2 cut(s) 435, 932
GsuI CTGGAG 2 cut(s) 542, 827
HaeIII GGCC 4 cut(s) 492, 789, 948, 953
HapII CCGG 2 cut(s) 489, 924
HhaI GCGC 1 cut(s) 644
Hin1II CATG 3 cut(s) 16, 895, 997
Hin6I GCGC 1 cut(s) 642
HinP1I GCGC 1 cut(s) 642
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 4 cut(s) 505, 690, 856, 920
HpaII CCGG 2 cut(s) 489, 924
HphI GGTGA 3 cut(s) 372, 547, 1148
Hpy166II GTNNAC 3 cut(s) 739, 828, 1051
Hpy188I TCNGA 5 cut(s) 29, 126, 472, 510, 602
Hpy188III TCNNGA 2 cut(s) 196, 913
Hpy8I GTNNAC 3 cut(s) 739, 828, 1051
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 3 cut(s) 1039, 1108, 1124
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4IV ACGT 1 cut(s) 867
HpyCH4V TGCA 3 cut(s) 853, 985, 1051
HpyF10VI GCNNNNNNNGC 3 cut(s) 295, 1024, 1027
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 867
Hsp92II CATG 3 cut(s) 16, 895, 997
HspAI GCGC 1 cut(s) 642
Kzo9I GATC 4 cut(s) 192, 438, 510, 900
LmnI GCTCC 4 cut(s) 10, 87, 669, 723
Lsp1109I GCAGC 5 cut(s) 276, 837, 969, 1005, 1036
MabI ACCWGGT 1 cut(s) 581
MaeI CTAG 2 cut(s) 44, 668
MaeII ACGT 1 cut(s) 867
MaeIII GTNAC 2 cut(s) 781, 1070
MalI GATC 4 cut(s) 194, 440, 512, 902
MboI GATC 4 cut(s) 192, 438, 510, 900
MhlI GDGCHC 5 cut(s) 131, 798, 873, 935, 1053
MluCI AATT 4 cut(s) 120, 164, 494, 1039
MlyI GAGTC 3 cut(s) 499, 850, 914
MmeI TCCRAC 6 cut(s) 141, 383, 451, 495, 523, 580
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 998
MspA1I CMGCKG 1 cut(s) 435
MspI CCGG 2 cut(s) 489, 924
MspR9I CCNGG 7 cut(s) 489, 521, 583, 845, 905, 925, 950
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 5 cut(s) 521, 583, 845, 905, 950
MvnI CGCG 1 cut(s) 974
MwoI GCNNNNNNNGC 3 cut(s) 295, 1024, 1027
NciI CCSGG 2 cut(s) 489, 925
NdeII GATC 4 cut(s) 192, 438, 510, 900
NlaIII CATG 3 cut(s) 16, 895, 997
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 840
NmuCI GTSAC 1 cut(s) 781
NsbI TGCGCA 1 cut(s) 643
NspI RCATGY 1 cut(s) 997
PaeR7I CTCGAG 1 cut(s) 791
PciI ACATGT 1 cut(s) 993
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 1 cut(s) 690
PflFI GACNNNGTC 1 cut(s) 464
PkrI GCNGC 8 cut(s) 94, 291, 852, 984, 1020, 1023, 1026, 1029
PleI GAGTC 3 cut(s) 499, 850, 914
PpsI GAGTC 3 cut(s) 499, 850, 914
Ppu21I YACGTR 1 cut(s) 868
PscI ACATGT 1 cut(s) 993
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 5 cut(s) 519, 581, 843, 903, 948
PspFI CCCAGC 2 cut(s) 431, 928
PspGI CCWGG 5 cut(s) 519, 581, 843, 903, 948
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 946, 952
PspXI VCTCGAGB 1 cut(s) 791
PsrI GAACNNNNNNTAC 2 cut(s) 8, 40
PstI CTGCAG 1 cut(s) 855
PstNI CAGNNNCTG 2 cut(s) 508, 850
PsyI GACNNNGTC 1 cut(s) 464
PvuII CAGCTG 1 cut(s) 435
RsaI GTAC 3 cut(s) 17, 36, 896
RsaNI GTAC 3 cut(s) 16, 35, 895
RseI CAYNNNNRTG 1 cut(s) 998
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 8 cut(s) 93, 290, 851, 983, 1019, 1022, 1025, 1028
Sau3AI GATC 4 cut(s) 192, 438, 510, 900
Sau96I GGNCC 2 cut(s) 946, 952
SchI GAGTC 3 cut(s) 499, 850, 914
ScrFI CCNGG 7 cut(s) 489, 521, 583, 845, 905, 925, 950
SduI GDGCHC 5 cut(s) 131, 798, 873, 935, 1053
SexAI ACCWGGT 1 cut(s) 581
SfcI CTRYAG 1 cut(s) 851
Sfr274I CTCGAG 1 cut(s) 791
SlaI CTCGAG 1 cut(s) 791
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 3 cut(s) 293, 791, 800
SmoI CTYRAG 3 cut(s) 293, 791, 800
Sse9I AATT 4 cut(s) 120, 164, 494, 1039
SsiI CCGC 6 cut(s) 93, 974, 1013, 1021, 1028, 1100
SspMI CTAG 2 cut(s) 44, 668
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 7 cut(s) 487, 519, 581, 843, 903, 923, 948
StyI CCWWGG 1 cut(s) 777
TaaI ACNGT 1 cut(s) 138
TaiI ACGT 1 cut(s) 870
TaqI TCGA 5 cut(s) 118, 441, 792, 914, 935
TaqII GACCGA 1 cut(s) 932
TasI AATT 4 cut(s) 120, 164, 494, 1039
TatI WGTACW 2 cut(s) 15, 894
TauI GCSGC 3 cut(s) 95, 1024, 1030
TfiI GAWTC 1 cut(s) 690
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 1 cut(s) 790
TseFI GTSAC 1 cut(s) 781
TseI GCWGC 5 cut(s) 289, 850, 982, 1018, 1024
Tsp45I GTSAC 1 cut(s) 781
TspDTI ATGAA 3 cut(s) 186, 252, 718
TspRI CASTG 1 cut(s) 790
Tth111I GACNNNGTC 1 cut(s) 464
VneI GTGCAC 1 cut(s) 1049
XapI RAATTY 1 cut(s) 120
XceI RCATGY 1 cut(s) 997
XhoI CTCGAG 1 cut(s) 791
XmiI GTMKAC 1 cut(s) 738
XspI CTAG 2 cut(s) 44, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.