MD01G1036700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015522
Physical Location & Seq
Reverse (-)
12325961 .. 12326581
621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1036700.v1.1.491

Sequence Viewer

Length: 621 bp
ATGGCTGGGTGGATATCTAATAACACAAAGTCGAATCCTAAAGCTGTTGACGGGAACGAAATGTTTCTGGGTCTAACTGCTGTTTCTCACAAGACTGCTGGCTTACACCTCATACAGAACTGCGATCTTCCTCCACCCTCGAAACTCTTTACGGGTTCGGGTCAAACTGTTGTGTCATCCATGAAAGGGGTTTGCCGCGTGAACTCGGATCGCGATAATGATGATCACACGGATGATCAGTGTGACACGGATGGTAACCAAATTGACGGCGAGAATGAGAAACTGGAGCTGCTGAAGGCGTTGAGGCTGTCGCAGACGAGGGCGAGAGAGGCGGAGAACAAGTCTGAGAGGTTGGAGAAGGAAAAAGACCGTCTCTCTAATGCCTTGCTTGTGGAGGCTAAAGAGTTGTTTGCTTACAAGCAATGGGTGAGGTTGCTTGAGCTTCAAGTTTCAAGGTTGCAGTCACATTGGGCGGAGGAAGACAATAATAAGGCAAGTTGTGGTTGTGGCAGATCGAAAGGAGACGATGATGGGGATGGCATCACTTGGGTTGTAGCTTTGGCGCTTTGTTTCGGCATTGCCGGCGTAGGATTCGCATTCGGATGCAGATTCTTGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

22.7

Weight (kDa)

5.29

Isoelectric Point (pI)

25.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015522)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20210
malus_domestica MD01G1036700.v1.1 MD15G1317900.v1.1
prunus_persica Prupe.6G193900_v2.0.a1
pyrus_communis pycom15g28050
rosa_chinensis RchiOBHm_Chr2g0112271
rosa_laevigata RLG00000017967
rosa_multiflora Rmu_sc0004467.1_g000025 Rmu_sc0011951.1_g000003
rosa_roxburghii Rroxscaffold_2G00131570
rosa_rugosa Rorug02G0179800
rosa_samantha Rh2AG232300 Rh2BG245600 Rh2CG236500 Rh2DG240000
rosa_wichuraiana Rw2G018010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 198, 213
AciI CCGC 3 cut(s) 196, 332, 473
AclWI GGATC 1 cut(s) 216
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 2 cut(s) 446, 453
AluBI AGCT 4 cut(s) 44, 289, 442, 557
AluI AGCT 4 cut(s) 44, 289, 442, 557
Alw26I GTCTC 2 cut(s) 377, 516
AlwI GGATC 1 cut(s) 216
ApeKI GCWGC 1 cut(s) 289
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 565
AsuHPI GGTGA 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 276
BccI CCATC 3 cut(s) 245, 524, 530
BceAI ACGGC 1 cut(s) 283
BclI TGATCA 2 cut(s) 223, 235
BcoDI GTCTC 2 cut(s) 377, 516
BfoI RGCGCY 1 cut(s) 566
BisI GCNGC 2 cut(s) 196, 290
BlsI GCNGC 2 cut(s) 197, 291
BmsI GCATC 2 cut(s) 549, 593
BpiI GAAGAC 1 cut(s) 486
BpmI CTGGAG 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 2 cut(s) 428, 576
BseGI GGATG 5 cut(s) 176, 238, 256, 541, 608
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMI GCAATG 2 cut(s) 428, 576
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 276
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 2 cut(s) 198, 213
BsiSI CCGG 1 cut(s) 582
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 2 cut(s) 377, 516
BsmBI CGTCTC 2 cut(s) 377, 516
BsmI GAATGC 1 cut(s) 596
Bsp143I GATC 5 cut(s) 124, 208, 223, 235, 512
Bsp68I TCGCGA 1 cut(s) 213
BspACI CCGC 3 cut(s) 196, 332, 473
BspCNI CTCAG 1 cut(s) 337
BspFNI CGCG 2 cut(s) 198, 213
BspPI GGATC 1 cut(s) 216
BsrDI GCAATG 2 cut(s) 428, 576
BsrFI RCCGGY 1 cut(s) 581
BsrI ACTGG 1 cut(s) 288
BssAI RCCGGY 1 cut(s) 581
BssMI GATC 5 cut(s) 124, 208, 223, 235, 512
Bst4CI ACNGT 2 cut(s) 169, 371
BstC8I GCNNGC 2 cut(s) 100, 583
BstDEI CTNAG 1 cut(s) 345
BstEII GGTNACC 1 cut(s) 254
BstF5I GGATG 5 cut(s) 176, 238, 256, 541, 608
BstFNI CGCG 2 cut(s) 198, 213
BstH2I RGCGCY 1 cut(s) 566
BstHHI GCGC 1 cut(s) 565
BstKTI GATC 5 cut(s) 127, 211, 226, 238, 515
BstMAI GTCTC 2 cut(s) 377, 516
BstMBI GATC 5 cut(s) 124, 208, 223, 235, 512
BstMWI GCNNNNNNNGC 2 cut(s) 329, 582
BstPI GGTNACC 1 cut(s) 254
BstUI CGCG 2 cut(s) 198, 213
BstV1I GCAGC 1 cut(s) 276
BstV2I GAAGAC 1 cut(s) 486
BtsCI GGATG 5 cut(s) 176, 238, 256, 541, 608
BtsIMutI CAGTG 1 cut(s) 245
BtuMI TCGCGA 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 100, 583
CfoI GCGC 1 cut(s) 565
Cfr10I RCCGGY 1 cut(s) 581
Csp6I GTAC 1 cut(s) 616
CviAII CATG 1 cut(s) 181
CviJI RGCY 8 cut(s) 5, 44, 102, 289, 307, 398, 442, 557
CviKI_1 RGCY 8 cut(s) 5, 44, 102, 289, 307, 398, 442, 557
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 1 cut(s) 345
DpnI GATC 5 cut(s) 126, 210, 225, 237, 514
DpnII GATC 5 cut(s) 124, 208, 223, 235, 512
EciI GGCGGA 2 cut(s) 347, 488
Eco32I GATATC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 314
Eco91I GGTNACC 1 cut(s) 254
EcoO65I GGTNACC 1 cut(s) 254
EcoRV GATATC 1 cut(s) 15
Esp3I CGTCTC 2 cut(s) 377, 516
FaeI CATG 1 cut(s) 184
FaiI YATR 2 cut(s) 113, 182
FatI CATG 1 cut(s) 180
FbaI TGATCA 2 cut(s) 223, 235
Fnu4HI GCNGC 2 cut(s) 196, 290
FokI GGATG 5 cut(s) 163, 245, 263, 548, 615
Fsp4HI GCNGC 2 cut(s) 196, 290
GlaI GCGC 1 cut(s) 564
GluI GCNGC 2 cut(s) 196, 290
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 566
HapII CCGG 1 cut(s) 582
HhaI GCGC 1 cut(s) 565
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 1 cut(s) 563
HinP1I GCGC 1 cut(s) 563
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 3 cut(s) 34, 591, 609
HpaII CCGG 1 cut(s) 582
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 2 cut(s) 49, 202
Hpy188I TCNGA 3 cut(s) 208, 346, 602
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 2 cut(s) 49, 202
HpyAV CCTTC 2 cut(s) 289, 352
HpyCH4III ACNGT 2 cut(s) 169, 371
HpyCH4V TGCA 2 cut(s) 460, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 582
HpyF3I CTNAG 1 cut(s) 345
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 1 cut(s) 563
KroI GCCGGC 1 cut(s) 581
KroNI GCCGGC 1 cut(s) 583
Ksp22I TGATCA 2 cut(s) 223, 235
Kzo9I GATC 5 cut(s) 124, 208, 223, 235, 512
LmnI GCTCC 1 cut(s) 286
LpnPI CCDG 4 cut(s) 53, 84, 269, 595
Lsp1109I GCAGC 1 cut(s) 276
LweI GCATC 2 cut(s) 549, 593
MaeIII GTNAC 3 cut(s) 242, 254, 462
MalI GATC 5 cut(s) 126, 210, 225, 237, 514
MboI GATC 5 cut(s) 124, 208, 223, 235, 512
MboII GAAGA 2 cut(s) 119, 491
MluCI AATT 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 333
MroNI GCCGGC 1 cut(s) 581
MroXI GAANNNNTTC 1 cut(s) 63
MslI CAYNNNNRTG 2 cut(s) 231, 601
MspI CCGG 1 cut(s) 582
Mva1269I GAATGC 1 cut(s) 596
MvnI CGCG 2 cut(s) 198, 213
MwoI GCNNNNNNNGC 2 cut(s) 329, 582
NaeI GCCGGC 1 cut(s) 583
NdeII GATC 5 cut(s) 124, 208, 223, 235, 512
NgoMIV GCCGGC 1 cut(s) 581
NlaIII CATG 1 cut(s) 184
NmuCI GTSAC 2 cut(s) 242, 462
NruI TCGCGA 1 cut(s) 213
PctI GAATGC 1 cut(s) 596
PdiI GCCGGC 1 cut(s) 583
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 3 cut(s) 34, 591, 609
PkrI GCNGC 2 cut(s) 197, 291
PspEI GGTNACC 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 5
RruI TCGCGA 1 cut(s) 213
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
RseI CAYNNNNRTG 2 cut(s) 231, 601
SatI GCNGC 2 cut(s) 196, 290
Sau3AI GATC 5 cut(s) 124, 208, 223, 235, 512
SetI ASST 8 cut(s) 46, 111, 291, 353, 434, 444, 458, 559
SfaNI GCATC 2 cut(s) 549, 593
SmiMI CAYNNNNRTG 2 cut(s) 231, 601
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 1 cut(s) 261
SsiI CCGC 3 cut(s) 196, 332, 473
TaaI ACNGT 2 cut(s) 169, 371
TaqI TCGA 3 cut(s) 32, 140, 515
TasI AATT 1 cut(s) 261
TatI WGTACW 1 cut(s) 615
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 3 cut(s) 34, 591, 609
TscAI CASTG 1 cut(s) 245
TseFI GTSAC 2 cut(s) 242, 462
TseI GCWGC 1 cut(s) 289
Tsp45I GTSAC 2 cut(s) 242, 462
TspDTI ATGAA 1 cut(s) 197
TspGWI ACGGA 2 cut(s) 245, 263
TspRI CASTG 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.