MD15G1317900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015522
Physical Location & Seq
Forward (+)
32799017 .. 32799634
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1317900.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGCTGGGCGGATATCTAATAACACAAGGTTGAATTCTAAAGCAGTTGATGGGAATGAAATGTTTCTGGGTCTAACTGCTGTTTCTCACAAGACTGCTGGCTTAGACCGCATACAGAACTGCGATCTTCCTCCGCCGGCAAAACTATTTACGGGTTCGGTTCAAACTATTGTGTCATCCATGAAAAGGATTTGCAGCGTGAACTCGGATCGCGACGCTGATCACAGGGACGATCAGTTTGACATGGATGGAAACGGAATTGACGGAGAGAATGAGAAGCTGGAGCTTCTGAAGGCGCTGAGGCTGTCGCAAACGAGAGCGAGGGAGGCGGAGAAGAAGGCTGAGAGGTTGGGGAAGGAGAAAGACTGTCTCGCTAATGCGTTGCTTGCGGAGGCTAAAGAGTTGTTTGCTTACAGGCAATGGGTGATGTTGCTTGAGCTTCAAGTTTCAAAGTTGCAGTCGCATTGGGCGGAGCAAGGGAGTGATCAGCCGAGTTGTGGTTGTGGCAGATCAAAAGGAGGTGACGGTGGGGATGGCATCACTTGGGTTGTAGCTTTGGCACTTTGTTTTGGCATCGCCGGCGTAGGCTTGGCATTCGGTTGCAGATACTTGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

206

Amino Acids

22.33

Weight (kDa)

6.32

Isoelectric Point (pI)

22.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015522)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20210
malus_domestica MD01G1036700.v1.1 MD15G1317900.v1.1
prunus_persica Prupe.6G193900_v2.0.a1
pyrus_communis pycom15g28050
rosa_chinensis RchiOBHm_Chr2g0112271
rosa_laevigata RLG00000017967
rosa_multiflora Rmu_sc0004467.1_g000025 Rmu_sc0011951.1_g000003
rosa_roxburghii Rroxscaffold_2G00131570
rosa_rugosa Rorug02G0179800
rosa_samantha Rh2AG232300 Rh2BG245600 Rh2CG236500 Rh2DG240000
rosa_wichuraiana Rw2G018010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 213
AciI CCGC 6 cut(s) 10, 109, 134, 329, 389, 470
AclWI GGATC 1 cut(s) 216
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 311
AfaI GTAC 1 cut(s) 614
AfiI CCNNNNNNNGG 2 cut(s) 186, 497
AgsI TTSAA 4 cut(s) 34, 164, 443, 450
AluBI AGCT 4 cut(s) 280, 286, 439, 554
AluI AGCT 4 cut(s) 280, 286, 439, 554
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 1 cut(s) 216
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 34
ArsI GACNNNNNNTTYG 4 cut(s) 221, 253, 443, 475
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 298
AsuHPI GGTGA 2 cut(s) 436, 533
BbvCI CCTCAGC 1 cut(s) 299
BbvI GCAGC 1 cut(s) 207
BccI CCATC 3 cut(s) 44, 242, 527
BclI TGATCA 2 cut(s) 220, 484
BcoDI GTCTC 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 299
BisI GCNGC 1 cut(s) 196
BlsI GCNGC 1 cut(s) 197
BmsI GCATC 2 cut(s) 546, 582
BpmI CTGGAG 1 cut(s) 302
Bpu10I CCTNAGC 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 455
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 497
Bse118I RCCGGY 2 cut(s) 136, 578
Bse3DI GCAATG 1 cut(s) 425
BseGI GGATG 3 cut(s) 176, 253, 538
BseLI CCNNNNNNNGG 2 cut(s) 186, 497
BseMI GCAATG 1 cut(s) 425
BseMII CTCAG 2 cut(s) 290, 333
BseXI GCAGC 1 cut(s) 207
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 213
BsiSI CCGG 2 cut(s) 137, 579
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 2 cut(s) 186, 497
BsmAI GTCTC 1 cut(s) 374
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 593
Bsp143I GATC 6 cut(s) 124, 208, 220, 232, 484, 509
Bsp68I TCGCGA 1 cut(s) 213
BspACI CCGC 6 cut(s) 10, 109, 134, 329, 389, 470
BspCNI CTCAG 2 cut(s) 291, 334
BspFNI CGCG 1 cut(s) 213
BspPI GGATC 1 cut(s) 216
BsrDI GCAATG 1 cut(s) 425
BsrFI RCCGGY 2 cut(s) 136, 578
BssAI RCCGGY 2 cut(s) 136, 578
BssMI GATC 6 cut(s) 124, 208, 220, 232, 484, 509
Bst4CI ACNGT 2 cut(s) 368, 527
BstC8I GCNNGC 4 cut(s) 100, 138, 387, 580
BstDEI CTNAG 3 cut(s) 103, 299, 342
BstF5I GGATG 3 cut(s) 176, 253, 538
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 6 cut(s) 127, 211, 223, 235, 487, 512
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 6 cut(s) 124, 208, 220, 232, 484, 509
BstMWI GCNNNNNNNGC 4 cut(s) 108, 326, 386, 579
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 559
BtsCI GGATG 3 cut(s) 176, 253, 538
BtuMI TCGCGA 1 cut(s) 213
Cac8I GCNNGC 4 cut(s) 100, 138, 387, 580
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 2 cut(s) 136, 578
CseI GACGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 613
CviAII CATG 2 cut(s) 181, 244
CviQI GTAC 1 cut(s) 613
DdeI CTNAG 3 cut(s) 103, 299, 342
DpnI GATC 6 cut(s) 126, 210, 222, 234, 486, 511
DpnII GATC 6 cut(s) 124, 208, 220, 232, 484, 509
EciI GGCGGA 4 cut(s) 25, 123, 344, 485
Eco32I GATATC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 311
EcoRI GAATTC 1 cut(s) 34
EcoRV GATATC 1 cut(s) 15
FaeI CATG 2 cut(s) 184, 247
FaiI YATR 3 cut(s) 113, 182, 245
FaqI GGGAC 1 cut(s) 242
FatI CATG 2 cut(s) 180, 243
FbaI TGATCA 2 cut(s) 220, 484
Fnu4HI GCNGC 1 cut(s) 196
FokI GGATG 3 cut(s) 163, 260, 545
Fsp4HI GCNGC 1 cut(s) 196
GlaI GCGC 1 cut(s) 297
GluI GCNGC 1 cut(s) 196
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 299
HapII CCGG 2 cut(s) 137, 579
HgaI GACGC 1 cut(s) 224
HhaI GCGC 1 cut(s) 298
Hin1II CATG 2 cut(s) 184, 247
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HpaII CCGG 2 cut(s) 137, 579
HphI GGTGA 2 cut(s) 436, 533
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 2 cut(s) 208, 291
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 3 cut(s) 286, 331, 349
HpyCH4III ACNGT 2 cut(s) 368, 527
HpyCH4V TGCA 3 cut(s) 195, 457, 603
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 326, 386, 579
HpyF3I CTNAG 3 cut(s) 103, 299, 342
Hsp92II CATG 2 cut(s) 184, 247
HspAI GCGC 1 cut(s) 296
KroI GCCGGC 2 cut(s) 136, 578
KroNI GCCGGC 2 cut(s) 138, 580
Ksp22I TGATCA 2 cut(s) 220, 484
Kzo9I GATC 6 cut(s) 124, 208, 220, 232, 484, 509
LmnI GCTCC 2 cut(s) 283, 472
LpnPI CCDG 7 cut(s) 53, 84, 150, 211, 266, 400, 592
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 2 cut(s) 546, 582
MaeIII GTNAC 1 cut(s) 521
MalI GATC 6 cut(s) 126, 210, 222, 234, 486, 511
MboI GATC 6 cut(s) 124, 208, 220, 232, 484, 509
MboII GAAGA 2 cut(s) 119, 346
MluCI AATT 2 cut(s) 34, 258
MnlI CCTC 7 cut(s) 141, 294, 315, 319, 339, 385, 512
MreI CGCCGGCG 1 cut(s) 578
MroNI GCCGGC 2 cut(s) 136, 578
MroXI GAANNNNTTC 1 cut(s) 63
MspI CCGG 2 cut(s) 137, 579
Mva1269I GAATGC 1 cut(s) 593
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 4 cut(s) 108, 326, 386, 579
NaeI GCCGGC 2 cut(s) 138, 580
NdeII GATC 6 cut(s) 124, 208, 220, 232, 484, 509
NgoMIV GCCGGC 2 cut(s) 136, 578
NlaIII CATG 2 cut(s) 184, 247
NmeAIII GCCGAG 1 cut(s) 516
NmuCI GTSAC 1 cut(s) 521
NruI TCGCGA 1 cut(s) 213
PctI GAATGC 1 cut(s) 593
PdiI GCCGGC 2 cut(s) 138, 580
PdmI GAANNNNTTC 1 cut(s) 63
PkrI GCNGC 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 5
RruI TCGCGA 1 cut(s) 213
RsaI GTAC 1 cut(s) 614
RsaNI GTAC 1 cut(s) 613
SatI GCNGC 1 cut(s) 196
Sau3AI GATC 6 cut(s) 124, 208, 220, 232, 484, 509
SetI ASST 7 cut(s) 32, 282, 288, 350, 441, 523, 556
SfaNI GCATC 2 cut(s) 546, 582
SgrAI CRCCGGYG 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 2 cut(s) 34, 258
SsiI CCGC 6 cut(s) 10, 109, 134, 329, 389, 470
TaaI ACNGT 2 cut(s) 368, 527
TasI AATT 2 cut(s) 34, 258
TatI WGTACW 1 cut(s) 612
TseFI GTSAC 1 cut(s) 521
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 1 cut(s) 521
TspDTI ATGAA 2 cut(s) 72, 197
TspGWI ACGGA 2 cut(s) 270, 279
XapI RAATTY 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.