MD01G1044100.v1.1

Serine hydroxymethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007608
Physical Location & Seq
Forward (+)
14341801 .. 14346792
4992 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1044100.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGGCGATGGCATTGGCGCTTCGCAGGCTTTCAGCTTCTGGGGATAAGTCCATTCGTCCCCTCTTCAATGCCACCTCTCACTATTACATGTCATCTTTGCCCAATGAAGCTGTCTATGAGAAGCCTGGTGTCACTTGGCCTAAGCAATTGAATGCTCCACTGGAGGCTGTGGATCCCGAAATTGCGGATATCATTGAGCACGAGAAAACCAGGCAATGGAAGGGTTTAGAGCTGATACCCTCGGAGAACTTCACTTCGGTCTCCGTCATGCAAGCAGTTGGGTCAGTCATGACCAACAAATACAGCGAAGGATATCCCGGTGCTAGATACTATGGAGGAAATGAGTTCATTGACATGGCAGAATCCTTATGTCAGAAGCGTGCTTTGGAAGCTTTTCGGTTGGATCCAGCAAAATGGGGAGTGAACGTGCAGCCTTTATCAGGATCTCCAGCTAATTTCCAGGTTTACACTGCATTGTTAAAACCTCATGACAGAATCATGGCACTTGATCTTCCTCACGGTGGTCATCTTTCTCACGGTTATCAGACTGACACTAAAAAGATATCTGCTGTGTCAATATTTTTCGAAACCATGCCATACAGACTGAATGAGAGCACCGGCTATATTGACTACGACCAGTTGGAGAAAAGTGCCACGCTATACCGGCCAAAATTAATAGTTGCTGGTGCTAGTGCTTATGCACGTCTGTATGATTATGACCGCATTCGTAAGGTATGCAACAAACAGAAAGCAATATTGTTGGCAGACATGGCACACATCAGTGGGTTGGTTGCAGCTGGTGTTATTCCATCACCTTTCGACTATGCAGATGTAGTGACCACCACAACGCACAAGTCACTGCGTGGCCCTCGTGGAGCCATGATTTTTTTTAGAAAGGGGGTAAAAGAGACTAACAAACAAGGGCAAGAGGTGTTGTACGACTACGAAGACAAAATCAATCAAGCTGTCTTTCCTGGACTTCAAGGTGGCCCACACAACCACACAATTACCGGTTTGGCAGTTGCCTTGAAACAGGCTACTACTCCAGAGTACAAAGCCTATCAGGAGCAAGTTCTCACCAATTGCTCAAGATTTGCCCAGTCTTTGGCTCAGAAAGGATATGAACTTGTTTCTGGCGGAACCGAGAACCATTTAGTTTTGGTGAATTTGAAGAACAAGGGAATTGACGGTTCCAGGGTCGAAAAGGTGTTGGAGGCCGTTCACATTGCAGCCAACAAAAACACTGTTCCTGGAGATGTGTCTGCTATGGTTCCTGGTGGCATCAGGATGGGAACCCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTCGCTAAGGTTGCAGACTTTTTTGATGCTGCTGTGAATTTGGCATTGAAGATCAAGGAGGAAGCCAAAGGTACAAAGTTGAAGGACTTTGTGGCCGCACTTTCTGCCCCTCACTTCCAATCTGAGACTGCAAAGCTCCGCCATGATGTTGAGGAGTATGCTAAGCAATTTCCTACAATTGGGTTTGAGAAAGAAACCTTGAAGTACAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001505 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004372 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0005886 GO:0006082 GO:0006520 GO:0006544 GO:0006563 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008150 GO:0008152 GO:0008187 GO:0008219 GO:0008266 GO:0009069 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009626 GO:0009628 GO:0009853 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0012501 GO:0016020 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019752 GO:0022626 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0033554 GO:0034050 GO:0042133 GO:0042221 GO:0043094 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045087 GO:0046686 GO:0048046 GO:0048511 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901605 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523
Pfam Domains
Protein Families
SHMT

Protein Analysis

516

Amino Acids

56.87

Weight (kDa)

8.39

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 54 - 451 5.5e-214 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 216, 1105
AciI CCGC 5 cut(s) 185, 721, 1137, 1428, 1471
AclWI GGATC 5 cut(s) 167, 180, 398, 411, 451
AcoI YGGCCR 2 cut(s) 665, 1425
AcsI RAATTY 2 cut(s) 1165, 1369
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 4 cut(s) 938, 1052, 1405, 1538
AfiI CCNNNNNNNGG 5 cut(s) 216, 440, 521, 1105, 1511
AflIII ACRYGT 1 cut(s) 87
AgeI ACCGGT 1 cut(s) 1010
AgsI TTSAA 8 cut(s) 67, 151, 983, 1030, 1171, 1381, 1414, 1534
AjiI CACGTC 1 cut(s) 704
AjnI CCWGG 7 cut(s) 124, 209, 459, 973, 1193, 1249, 1273
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AleI CACNNNNGTG 1 cut(s) 780
AluBI AGCT 8 cut(s) 35, 110, 232, 392, 452, 797, 965, 1468
AluI AGCT 8 cut(s) 35, 110, 232, 392, 452, 797, 965, 1468
Alw21I GWGCWC 2 cut(s) 201, 617
Alw26I GTCTC 3 cut(s) 265, 902, 1451
AlwI GGATC 5 cut(s) 167, 180, 398, 411, 451
AlwNI CAGNNNCTG 1 cut(s) 38
AoxI GGCC 6 cut(s) 137, 665, 865, 988, 1215, 1425
ApeKI GCWGC 4 cut(s) 430, 794, 1229, 1361
ApoI RAATTY 2 cut(s) 1165, 1369
AseI ATTAAT 1 cut(s) 674
AsiGI ACCGGT 1 cut(s) 1010
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 866, 989
AsuC2I CCSGG 2 cut(s) 318, 1298
AsuHPI GGTGA 3 cut(s) 804, 1069, 1174
AsuII TTCGAA 1 cut(s) 585
BamHI GGATCC 2 cut(s) 172, 403
BauI CACGAG 2 cut(s) 200, 870
BbsI GAAGAC 1 cut(s) 954
Bbv12I GWGCWC 2 cut(s) 201, 617
BbvI GCAGC 4 cut(s) 442, 806, 1241, 1348
BccI CCATC 2 cut(s) 817, 1282
BceAI ACGGC 1 cut(s) 1202
BciT130I CCWGG 7 cut(s) 126, 211, 461, 975, 1195, 1251, 1275
BcnI CCSGG 2 cut(s) 318, 1298
BcoDI GTCTC 3 cut(s) 265, 902, 1451
BfaI CTAG 3 cut(s) 324, 690, 1311
BfoI RGCGCY 1 cut(s) 20
BisI GCNGC 5 cut(s) 431, 795, 1230, 1362, 1428
BlpI GCTNAGC 1 cut(s) 1494
BlsI GCNGC 5 cut(s) 432, 796, 1231, 1363, 1429
Bme1390I CCNGG 9 cut(s) 126, 211, 318, 461, 975, 1195, 1251, 1275, 1298
BmgBI CACGTC 1 cut(s) 704
BmgT120I GGNCC 2 cut(s) 866, 989
BmiI GGNNCC 7 cut(s) 174, 405, 877, 1141, 1192, 1272, 1294
BmrFI CCNGG 9 cut(s) 126, 211, 318, 461, 975, 1195, 1251, 1275, 1298
BmrI ACTGGG 1 cut(s) 1093
BmsI GCATC 2 cut(s) 1290, 1348
BmuI ACTGGG 1 cut(s) 1093
BpiI GAAGAC 1 cut(s) 954
BpmI CTGGAG 4 cut(s) 182, 432, 1029, 1272
Bpu10I CCTNAGC 2 cut(s) 141, 1338
Bpu1102I GCTNAGC 1 cut(s) 1494
Bpu14I TTCGAA 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 1072
BpuMI CCSGG 2 cut(s) 318, 1298
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 3 cut(s) 240, 1194, 1296
BsaWI WCCGGW 1 cut(s) 1010
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 440, 521, 1105, 1511
Bse118I RCCGGY 3 cut(s) 617, 663, 1010
Bse1I ACTGG 3 cut(s) 165, 637, 1099
Bse3DI GCAATG 2 cut(s) 221, 1224
BseBI CCWGG 7 cut(s) 126, 211, 461, 975, 1195, 1251, 1275
BseDI CCNNGG 3 cut(s) 240, 1194, 1296
BseGI GGATG 1 cut(s) 1293
BseLI CCNNNNNNNGG 5 cut(s) 216, 440, 521, 1105, 1511
BseMI GCAATG 2 cut(s) 221, 1224
BseMII CTCAG 2 cut(s) 1124, 1446
BseNI ACTGG 3 cut(s) 165, 637, 1099
BseRI GAGGAG 2 cut(s) 1340, 1499
BseXI GCAGC 4 cut(s) 442, 806, 1241, 1348
BsgI GTGCAG 1 cut(s) 449
BshFI GGCC 6 cut(s) 139, 667, 867, 990, 1217, 1427
BshTI ACCGGT 1 cut(s) 1010
BsiHKAI GWGCWC 2 cut(s) 201, 617
BsiSI CCGG 5 cut(s) 318, 618, 664, 1011, 1298
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 5 cut(s) 216, 440, 521, 1105, 1511
BsmAI GTCTC 3 cut(s) 265, 902, 1451
BsmFI GGGAC 1 cut(s) 42
BsmI GAATGC 2 cut(s) 157, 723
BsnI GGCC 6 cut(s) 139, 667, 867, 990, 1217, 1427
Bso31I GGTCTC 1 cut(s) 265
Bsp119I TTCGAA 1 cut(s) 585
Bsp1286I GDGCHC 2 cut(s) 201, 617
Bsp143I GATC 5 cut(s) 172, 403, 443, 508, 1383
Bsp1720I GCTNAGC 1 cut(s) 1494
BspACI CCGC 5 cut(s) 185, 721, 1137, 1428, 1471
BspANI GGCC 6 cut(s) 139, 667, 867, 990, 1217, 1427
BspCNI CTCAG 2 cut(s) 1123, 1447
BspHI TCATGA 2 cut(s) 288, 487
BspLI GGNNCC 7 cut(s) 174, 405, 877, 1141, 1192, 1272, 1294
BspPI GGATC 5 cut(s) 167, 180, 398, 411, 451
BspT104I TTCGAA 1 cut(s) 585
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 2 cut(s) 221, 1224
BsrFI RCCGGY 3 cut(s) 617, 663, 1010
BsrI ACTGG 3 cut(s) 165, 637, 1099
BssAI RCCGGY 3 cut(s) 617, 663, 1010
BssECI CCNNGG 3 cut(s) 240, 1194, 1296
BssMI GATC 5 cut(s) 172, 403, 443, 508, 1383
BssSI CACGAG 2 cut(s) 200, 870
Bst2BI CACGAG 2 cut(s) 200, 870
Bst2UI CCWGG 7 cut(s) 126, 211, 461, 975, 1195, 1251, 1275
Bst4CI ACNGT 4 cut(s) 521, 539, 1190, 1246
Bst6I CTCTTC 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 1436
BstBI TTCGAA 1 cut(s) 585
BstC8I GCNNGC 3 cut(s) 26, 273, 381
BstDEI CTNAG 5 cut(s) 141, 1110, 1338, 1455, 1494
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 1293
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 5 cut(s) 175, 406, 446, 511, 1386
BstMAI GTCTC 3 cut(s) 265, 902, 1451
BstMBI GATC 5 cut(s) 172, 403, 443, 508, 1383
BstMWI GCNNNNNNNGC 6 cut(s) 25, 389, 664, 770, 1343, 1436
BstNI CCWGG 7 cut(s) 126, 211, 461, 975, 1195, 1251, 1275
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 9 cut(s) 124, 209, 316, 459, 973, 1193, 1249, 1273, 1296
BstV1I GCAGC 4 cut(s) 442, 806, 1241, 1348
BstV2I GAAGAC 1 cut(s) 954
BstX2I RGATCY 3 cut(s) 172, 403, 443
BstXI CCANNNNNNTGG 1 cut(s) 414
BstYI RGATCY 3 cut(s) 172, 403, 443
BsuRI GGCC 6 cut(s) 139, 667, 867, 990, 1217, 1427
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 1 cut(s) 704
BtsCI GGATG 1 cut(s) 1293
BtsI GCAGTG 2 cut(s) 468, 857
BtsIMutI CAGTG 5 cut(s) 158, 468, 787, 857, 1242
Cac8I GCNNGC 3 cut(s) 26, 273, 381
CaiI CAGNNNCTG 1 cut(s) 38
CciI TCATGA 2 cut(s) 288, 487
CfoI GCGC 1 cut(s) 19
Cfr10I RCCGGY 3 cut(s) 617, 663, 1010
Cfr13I GGNCC 2 cut(s) 866, 989
Csp6I GTAC 4 cut(s) 937, 1051, 1404, 1537
CspAI ACCGGT 1 cut(s) 1010
CviQI GTAC 4 cut(s) 937, 1051, 1404, 1537
DdeI CTNAG 5 cut(s) 141, 1110, 1338, 1455, 1494
DpnI GATC 5 cut(s) 174, 405, 445, 510, 1385
DpnII GATC 5 cut(s) 172, 403, 443, 508, 1383
DraIII CACNNNGTG 1 cut(s) 863
EaeI YGGCCR 2 cut(s) 665, 1425
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 2 cut(s) 1152, 1460
Eco31I GGTCTC 1 cut(s) 265
Eco32I GATATC 3 cut(s) 190, 314, 564
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoRII CCWGG 7 cut(s) 124, 209, 459, 973, 1193, 1249, 1273
EcoRV GATATC 3 cut(s) 190, 314, 564
FaqI GGGAC 1 cut(s) 42
Fnu4HI GCNGC 5 cut(s) 431, 795, 1230, 1362, 1428
FokI GGATG 1 cut(s) 1300
Fsp4HI GCNGC 5 cut(s) 431, 795, 1230, 1362, 1428
FspBI CTAG 3 cut(s) 324, 690, 1311
GlaI GCGC 1 cut(s) 18
GluI GCNGC 5 cut(s) 431, 795, 1230, 1362, 1428
GsuI CTGGAG 4 cut(s) 182, 432, 1029, 1272
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 6 cut(s) 139, 667, 867, 990, 1217, 1427
HapII CCGG 5 cut(s) 318, 618, 664, 1011, 1298
HhaI GCGC 1 cut(s) 19
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 2 cut(s) 362, 495
HpaII CCGG 5 cut(s) 318, 618, 664, 1011, 1298
HphI GGTGA 3 cut(s) 804, 1069, 1174
Hpy166II GTNNAC 3 cut(s) 424, 466, 1222
Hpy188I TCNGA 5 cut(s) 244, 375, 546, 1113, 1456
Hpy188III TCNNGA 8 cut(s) 176, 289, 441, 488, 1046, 1064, 1089, 1285
Hpy8I GTNNAC 3 cut(s) 424, 466, 1222
HpyAV CCTTC 3 cut(s) 214, 302, 1408
HpyCH4III ACNGT 4 cut(s) 521, 539, 1190, 1246
HpyCH4IV ACGT 2 cut(s) 426, 703
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 389, 664, 770, 1343, 1436
HpyF3I CTNAG 5 cut(s) 141, 1110, 1338, 1455, 1494
HpySE526I ACGT 2 cut(s) 426, 703
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 5 cut(s) 172, 403, 443, 508, 1383
LmnI GCTCC 4 cut(s) 160, 875, 1066, 1473
Lsp1109I GCAGC 4 cut(s) 442, 806, 1241, 1348
LweI GCATC 2 cut(s) 1290, 1348
MaeI CTAG 3 cut(s) 324, 690, 1311
MaeII ACGT 2 cut(s) 426, 703
MaeIII GTNAC 3 cut(s) 130, 835, 855
MalI GATC 5 cut(s) 174, 405, 445, 510, 1385
MboI GATC 5 cut(s) 172, 403, 443, 508, 1383
MboII GAAGA 5 cut(s) 55, 503, 959, 1183, 1393
MfeI CAATTG 3 cut(s) 146, 1081, 1509
MflI RGATCY 3 cut(s) 172, 403, 443
MhlI GDGCHC 2 cut(s) 201, 617
MmeI TCCRAC 3 cut(s) 381, 621, 1191
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 2 cut(s) 479, 674
MslI CAYNNNNRTG 3 cut(s) 353, 780, 1286
MspA1I CMGCKG 1 cut(s) 797
MspI CCGG 5 cut(s) 318, 618, 664, 1011, 1298
MspR9I CCNGG 9 cut(s) 126, 211, 318, 461, 975, 1195, 1251, 1275, 1298
MunI CAATTG 3 cut(s) 146, 1081, 1509
Mva1269I GAATGC 2 cut(s) 157, 723
MvaI CCWGG 7 cut(s) 126, 211, 461, 975, 1195, 1251, 1275
MwoI GCNNNNNNNGC 6 cut(s) 25, 389, 664, 770, 1343, 1436
NciI CCSGG 2 cut(s) 318, 1298
NdeII GATC 5 cut(s) 172, 403, 443, 508, 1383
NlaIV GGNNCC 7 cut(s) 174, 405, 877, 1141, 1192, 1272, 1294
NmuCI GTSAC 3 cut(s) 130, 835, 855
NspI RCATGY 1 cut(s) 91
NspV TTCGAA 1 cut(s) 585
OliI CACNNNNGTG 1 cut(s) 780
PagI TCATGA 2 cut(s) 288, 487
PciI ACATGT 1 cut(s) 87
PctI GAATGC 2 cut(s) 157, 723
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 2 cut(s) 362, 495
PflMI CCANNNNNTGG 2 cut(s) 216, 1105
PfoI TCCNGGA 2 cut(s) 973, 1249
PinAI ACCGGT 1 cut(s) 1010
PkrI GCNGC 5 cut(s) 432, 796, 1231, 1363, 1429
PscI ACATGT 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 674
Psp6I CCWGG 7 cut(s) 124, 209, 459, 973, 1193, 1249, 1273
PspGI CCWGG 7 cut(s) 124, 209, 459, 973, 1193, 1249, 1273
PspN4I GGNNCC 7 cut(s) 174, 405, 877, 1141, 1192, 1272, 1294
PspPI GGNCC 2 cut(s) 866, 989
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 3 cut(s) 172, 403, 443
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 4 cut(s) 938, 1052, 1405, 1538
RsaNI GTAC 4 cut(s) 937, 1051, 1404, 1537
RseI CAYNNNNRTG 3 cut(s) 353, 780, 1286
SaqAI TTAA 2 cut(s) 479, 674
SatI GCNGC 5 cut(s) 431, 795, 1230, 1362, 1428
Sau3AI GATC 5 cut(s) 172, 403, 443, 508, 1383
Sau96I GGNCC 2 cut(s) 866, 989
ScrFI CCNGG 9 cut(s) 126, 211, 318, 461, 975, 1195, 1251, 1275, 1298
SduI GDGCHC 2 cut(s) 201, 617
SfaNI GCATC 2 cut(s) 1290, 1348
SfuI TTCGAA 1 cut(s) 585
SmiMI CAYNNNNRTG 3 cut(s) 353, 780, 1286
SmlI CTYRAG 1 cut(s) 1087
SmoI CTYRAG 1 cut(s) 1087
SsiI CCGC 5 cut(s) 185, 721, 1137, 1428, 1471
SspI AATATT 2 cut(s) 579, 756
SspMI CTAG 3 cut(s) 324, 690, 1311
StyD4I CCNGG 9 cut(s) 124, 209, 316, 459, 973, 1193, 1249, 1273, 1296
TaaI ACNGT 4 cut(s) 521, 539, 1190, 1246
TaiI ACGT 2 cut(s) 429, 706
TaqI TCGA 3 cut(s) 585, 819, 1200
TaqII GACCGA 1 cut(s) 247
TatI WGTACW 2 cut(s) 1050, 1536
TauI GCSGC 1 cut(s) 1430
TfiI GAWTC 2 cut(s) 362, 495
Tru1I TTAA 2 cut(s) 479, 674
Tru9I TTAA 2 cut(s) 479, 674
TscAI CASTG 5 cut(s) 165, 475, 787, 864, 1249
TseFI GTSAC 3 cut(s) 130, 835, 855
TseI GCWGC 4 cut(s) 430, 794, 1229, 1361
Tsp45I GTSAC 3 cut(s) 130, 835, 855
TspDTI ATGAA 3 cut(s) 120, 337, 1137
TspGWI ACGGA 1 cut(s) 253
TspRI CASTG 5 cut(s) 165, 475, 787, 864, 1249
Van91I CCANNNNNTGG 2 cut(s) 216, 1105
VspI ATTAAT 1 cut(s) 674
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 2 cut(s) 1165, 1369
XceI RCATGY 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 3 cut(s) 324, 690, 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.