pycom01g07110

Serine hydroxymethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007608
Physical Location & Seq
Forward (+)
7746232 .. 7750178
3947 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07110.7

Sequence Viewer

Length: 1512 bp
ATGGCGATGGCATTGGCGCTTCGCAGGCTTTCGGCTTCTGGGGATAAGTCCATTCGTCCCCTCTTCAATGCCACCTCTCACTATTACATGTCATCTTTGCCTAATGAAGCTGTCTATGAGAAGCCTGGTGTCAGTTGGCCTAAGCAATTGAATGCTCCACTGGAGGCTGTGGATCCCGAAATCGCGGATATCATTGAGCACGAGAAAACCAGGCAATGGAAGGGTTTAGAGCTGATACCCTCAGAGAACTTCACATCGGTCTCTGTCATGCAAGCAGTTGGGTCAGTCATGACCAACAAATACAGCGAAGGATATCCTGGTGCTAGATACTATGGAGGAAATGAGTTCATTGACATGGCAGAATCCTTATGTCAGAAGCGTGCTTTGGAAGCTTTTCGGTTGGCGGCGATCCAGCAAAATGGGGAGGTAATTCATATGGCATGTCTCATGAGCAGCCTCCTGCTATTTGTTACAATGTCTAGACCTTTATCAGGATCTCCAGCTAATTTCCAGGTTTACACTGCGTTGTTAAAACCTCATGACAGAATCATGGCACTTGATCTTCCTCATGGTGGTCATCTTTCTCACGGTTATCAGACTGACACTAAAAAGATATCTGCTGTGTCAACATTTTTCGAAACCATGCCATACAGACTGAATGAGAGCACTGGCTATATTGACTACGCCCAGTTGGAGAAAAGTGCCACGCTATACCGGCCAAAATTAATAGTTGCTGGTGCTAGTGCTTATGCACGTCTATATGATTATGACCGCATTCCTGGTGTTATTCCATCACCTTTCGATTATGCAGATGTAGTGACCACCACAACGCACAAGTCACTGCGTGGCCCTCGTGGAGCCATGATTTTTTTTAGAAAGGGGGTAAAAGAGACTAACAAACAAGGGCAAGAGGTGTTGTACGACTACGAAGACAAAATCAATCAAGCTGTCTTTCCTGGACTTCAAGGTGGCCCACACAACCACACAATTACTGGTTTGGCAGTTGCCTTGAAACAGGCTACTACTCCAGAGTACAAAGCCTATCAGGAGCAAGTTCTCACCAATTGCTCAAGATTTGCCCAGTCTTTGGCTCAGAAAGGATATGAACTTGTTTCTGGCGGAACCGAGAACCATTTAGTTTTGGGAATTGACGGTTCCAGGGTCGAAAAGGTGTTGGAGGCTGTTCACATTGCGGCCAACAAAAACACTGTTCCTGGAGATGTGTCAGCTATGGTTCCTGGTGGCATCAGGATGGGAACCCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTCGCTAAGGTTGCAGACTTTTTTGATGCTGCTGTGAATTTGGCATTGAAGATCAAGGGGGAAGCCAAAGGAACGAAGTTGAAGGACTTCGTGGCCGCACTTTCTGCCCCTCACTTCCAATCTGAGACTGCAAAGCTCCGCCACGATGTTGAGGAGTATGCTAAGCAATTTCCTACAATTGGGTTTGAGAAAGAAACCTTGAAGTACAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001505 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004372 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0005886 GO:0006082 GO:0006520 GO:0006544 GO:0006563 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008150 GO:0008152 GO:0008187 GO:0008219 GO:0008266 GO:0009069 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009626 GO:0009628 GO:0009853 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0012501 GO:0016020 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019752 GO:0022626 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0033554 GO:0034050 GO:0042133 GO:0042221 GO:0043094 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045087 GO:0046686 GO:0048046 GO:0048511 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901605 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523
Pfam Domains
Protein Families
SHMT

Protein Analysis

504

Amino Acids

55.45

Weight (kDa)

7.24

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 216, 1087
AccII CGCG 1 cut(s) 185
AciI CCGC 7 cut(s) 185, 404, 772, 1119, 1193, 1392, 1435
AclWI GGATC 4 cut(s) 167, 180, 403, 502
AcoI YGGCCR 3 cut(s) 716, 1194, 1389
AcsI RAATTY 1 cut(s) 1333
AdeI CACNNNGTG 1 cut(s) 845
AfaI GTAC 3 cut(s) 920, 1034, 1502
AfiI CCNNNNNNNGG 5 cut(s) 216, 491, 572, 1087, 1475
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 7 cut(s) 67, 151, 965, 1012, 1345, 1378, 1498
AjiI CACGTC 1 cut(s) 755
AjnI CCWGG 9 cut(s) 124, 209, 316, 510, 778, 955, 1157, 1213, 1237
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 7 cut(s) 110, 232, 392, 503, 947, 1229, 1432
AluI AGCT 7 cut(s) 110, 232, 392, 503, 947, 1229, 1432
Alw21I GWGCWC 2 cut(s) 201, 668
Alw26I GTCTC 4 cut(s) 265, 449, 884, 1415
AlwI GGATC 4 cut(s) 167, 180, 403, 502
AoxI GGCC 6 cut(s) 137, 716, 847, 970, 1194, 1389
ApeKI GCWGC 2 cut(s) 453, 1325
ApoI RAATTY 1 cut(s) 1333
AseI ATTAAT 1 cut(s) 725
Asp700I GAANNNNTTC 2 cut(s) 393, 1382
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 848, 971
AsuC2I CCSGG 1 cut(s) 1262
AsuHPI GGTGA 2 cut(s) 786, 1051
AsuII TTCGAA 1 cut(s) 636
BamHI GGATCC 1 cut(s) 172
BauI CACGAG 2 cut(s) 200, 852
BbsI GAAGAC 1 cut(s) 936
Bbv12I GWGCWC 2 cut(s) 201, 668
BbvI GCAGC 2 cut(s) 465, 1312
BccI CCATC 2 cut(s) 799, 1246
BciT130I CCWGG 9 cut(s) 126, 211, 318, 512, 780, 957, 1159, 1215, 1239
BcnI CCSGG 1 cut(s) 1262
BcoDI GTCTC 4 cut(s) 265, 449, 884, 1415
BfaI CTAG 4 cut(s) 324, 480, 741, 1275
BfoI RGCGCY 1 cut(s) 20
BisI GCNGC 5 cut(s) 405, 454, 1194, 1326, 1392
BlpI GCTNAGC 1 cut(s) 1458
BlsI GCNGC 5 cut(s) 406, 455, 1195, 1327, 1393
BmgBI CACGTC 1 cut(s) 755
BmgT120I GGNCC 2 cut(s) 848, 971
BmiI GGNNCC 6 cut(s) 174, 859, 1123, 1156, 1236, 1258
BmrI ACTGGG 2 cut(s) 682, 1075
BmsI GCATC 2 cut(s) 1254, 1312
BmuI ACTGGG 2 cut(s) 682, 1075
BpiI GAAGAC 1 cut(s) 936
BpmI CTGGAG 4 cut(s) 182, 483, 1011, 1236
Bpu10I CCTNAGC 2 cut(s) 141, 1302
Bpu1102I GCTNAGC 1 cut(s) 1458
Bpu14I TTCGAA 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 1054
BpuMI CCSGG 1 cut(s) 1262
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 1158, 1260
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 491, 572, 1087, 1475
Bse118I RCCGGY 1 cut(s) 714
Bse1I ACTGG 5 cut(s) 165, 673, 688, 997, 1081
Bse3DI GCAATG 2 cut(s) 221, 1188
BseBI CCWGG 9 cut(s) 126, 211, 318, 512, 780, 957, 1159, 1215, 1239
BseDI CCNNGG 2 cut(s) 1158, 1260
BseGI GGATG 1 cut(s) 1257
BseLI CCNNNNNNNGG 5 cut(s) 216, 491, 572, 1087, 1475
BseMI GCAATG 2 cut(s) 221, 1188
BseMII CTCAG 3 cut(s) 255, 1106, 1410
BseNI ACTGG 5 cut(s) 165, 673, 688, 997, 1081
BseRI GAGGAG 2 cut(s) 1304, 1463
BseXI GCAGC 2 cut(s) 465, 1312
Bsh1236I CGCG 1 cut(s) 185
BshFI GGCC 6 cut(s) 139, 718, 849, 972, 1196, 1391
BsiHKAI GWGCWC 2 cut(s) 201, 668
BsiSI CCGG 2 cut(s) 715, 1262
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 5 cut(s) 216, 491, 572, 1087, 1475
BsmAI GTCTC 4 cut(s) 265, 449, 884, 1415
BsmFI GGGAC 1 cut(s) 42
BsmI GAATGC 2 cut(s) 157, 774
BsnI GGCC 6 cut(s) 139, 718, 849, 972, 1196, 1391
Bso31I GGTCTC 1 cut(s) 265
Bsp119I TTCGAA 1 cut(s) 636
Bsp1286I GDGCHC 2 cut(s) 201, 668
Bsp143I GATC 5 cut(s) 172, 408, 494, 559, 1347
Bsp1720I GCTNAGC 1 cut(s) 1458
BspACI CCGC 7 cut(s) 185, 404, 772, 1119, 1193, 1392, 1435
BspANI GGCC 6 cut(s) 139, 718, 849, 972, 1196, 1391
BspCNI CTCAG 3 cut(s) 254, 1105, 1411
BspFNI CGCG 1 cut(s) 185
BspHI TCATGA 3 cut(s) 288, 447, 538
BspLI GGNNCC 6 cut(s) 174, 859, 1123, 1156, 1236, 1258
BspPI GGATC 4 cut(s) 167, 180, 403, 502
BspT104I TTCGAA 1 cut(s) 636
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 2 cut(s) 221, 1188
BsrFI RCCGGY 1 cut(s) 714
BsrI ACTGG 5 cut(s) 165, 673, 688, 997, 1081
BssAI RCCGGY 1 cut(s) 714
BssECI CCNNGG 2 cut(s) 1158, 1260
BssMI GATC 5 cut(s) 172, 408, 494, 559, 1347
BssSI CACGAG 2 cut(s) 200, 852
Bst2BI CACGAG 2 cut(s) 200, 852
Bst2UI CCWGG 9 cut(s) 126, 211, 318, 512, 780, 957, 1159, 1215, 1239
Bst4CI ACNGT 3 cut(s) 590, 1154, 1210
Bst6I CTCTTC 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 1400
BstBI TTCGAA 1 cut(s) 636
BstC8I GCNNGC 3 cut(s) 26, 273, 381
BstDEI CTNAG 6 cut(s) 141, 241, 1092, 1302, 1419, 1458
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 1 cut(s) 1257
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 5 cut(s) 175, 411, 497, 562, 1350
BstMAI GTCTC 4 cut(s) 265, 449, 884, 1415
BstMBI GATC 5 cut(s) 172, 408, 494, 559, 1347
BstMWI GCNNNNNNNGC 5 cut(s) 25, 389, 715, 1307, 1400
BstNI CCWGG 9 cut(s) 126, 211, 318, 512, 780, 957, 1159, 1215, 1239
BstNSI RCATGY 2 cut(s) 91, 444
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 2 cut(s) 465, 1312
BstV2I GAAGAC 1 cut(s) 936
BstX2I RGATCY 2 cut(s) 172, 494
BstXI CCANNNNNNTGG 1 cut(s) 419
BstYI RGATCY 2 cut(s) 172, 494
BsuRI GGCC 6 cut(s) 139, 718, 849, 972, 1196, 1391
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 1 cut(s) 755
BtsCI GGATG 1 cut(s) 1257
BtsI GCAGTG 2 cut(s) 519, 839
BtsIMutI CAGTG 5 cut(s) 158, 519, 666, 839, 1206
Cac8I GCNNGC 3 cut(s) 26, 273, 381
CciI TCATGA 3 cut(s) 288, 447, 538
CfoI GCGC 1 cut(s) 19
Cfr10I RCCGGY 1 cut(s) 714
Cfr13I GGNCC 2 cut(s) 848, 971
Csp6I GTAC 3 cut(s) 919, 1033, 1501
CviQI GTAC 3 cut(s) 919, 1033, 1501
DdeI CTNAG 6 cut(s) 141, 241, 1092, 1302, 1419, 1458
DpnI GATC 5 cut(s) 174, 410, 496, 561, 1349
DpnII GATC 5 cut(s) 172, 408, 494, 559, 1347
DraIII CACNNNGTG 1 cut(s) 845
EaeI YGGCCR 3 cut(s) 716, 1194, 1389
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 2 cut(s) 1134, 1424
Eco31I GGTCTC 1 cut(s) 265
Eco32I GATATC 3 cut(s) 190, 314, 615
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoRII CCWGG 9 cut(s) 124, 209, 316, 510, 778, 955, 1157, 1213, 1237
EcoRV GATATC 3 cut(s) 190, 314, 615
FaqI GGGAC 1 cut(s) 42
FauNDI CATATG 1 cut(s) 435
Fnu4HI GCNGC 5 cut(s) 405, 454, 1194, 1326, 1392
FokI GGATG 1 cut(s) 1264
Fsp4HI GCNGC 5 cut(s) 405, 454, 1194, 1326, 1392
FspBI CTAG 4 cut(s) 324, 480, 741, 1275
GlaI GCGC 1 cut(s) 18
GluI GCNGC 5 cut(s) 405, 454, 1194, 1326, 1392
GsuI CTGGAG 4 cut(s) 182, 483, 1011, 1236
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 6 cut(s) 139, 718, 849, 972, 1196, 1391
HapII CCGG 2 cut(s) 715, 1262
HhaI GCGC 1 cut(s) 19
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HincII GTYRAC 1 cut(s) 627
HindII GTYRAC 1 cut(s) 627
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 2 cut(s) 362, 546
HpaII CCGG 2 cut(s) 715, 1262
HphI GGTGA 2 cut(s) 786, 1051
Hpy166II GTNNAC 3 cut(s) 517, 627, 1186
Hpy188I TCNGA 5 cut(s) 244, 375, 597, 1095, 1420
Hpy8I GTNNAC 3 cut(s) 517, 627, 1186
HpyAV CCTTC 3 cut(s) 214, 302, 1372
HpyCH4III ACNGT 3 cut(s) 590, 1154, 1210
HpyCH4IV ACGT 1 cut(s) 754
HpyCH4V TGCA 5 cut(s) 271, 752, 809, 1310, 1427
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 389, 715, 1307, 1400
HpyF3I CTNAG 6 cut(s) 141, 241, 1092, 1302, 1419, 1458
HpySE526I ACGT 1 cut(s) 754
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 5 cut(s) 172, 408, 494, 559, 1347
LmnI GCTCC 4 cut(s) 160, 857, 1048, 1437
Lsp1109I GCAGC 2 cut(s) 465, 1312
LweI GCATC 2 cut(s) 1254, 1312
MaeI CTAG 4 cut(s) 324, 480, 741, 1275
MaeII ACGT 1 cut(s) 754
MaeIII GTNAC 3 cut(s) 469, 817, 837
MalI GATC 5 cut(s) 174, 410, 496, 561, 1349
MboI GATC 5 cut(s) 172, 408, 494, 559, 1347
MboII GAAGA 4 cut(s) 55, 554, 941, 1357
MfeI CAATTG 3 cut(s) 146, 1063, 1473
MflI RGATCY 2 cut(s) 172, 494
MhlI GDGCHC 2 cut(s) 201, 668
MmeI TCCRAC 2 cut(s) 672, 1155
MroXI GAANNNNTTC 2 cut(s) 393, 1382
MseI TTAA 2 cut(s) 530, 725
MslI CAYNNNNRTG 2 cut(s) 353, 1250
MspI CCGG 2 cut(s) 715, 1262
MunI CAATTG 3 cut(s) 146, 1063, 1473
Mva1269I GAATGC 2 cut(s) 157, 774
MvaI CCWGG 9 cut(s) 126, 211, 318, 512, 780, 957, 1159, 1215, 1239
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 5 cut(s) 25, 389, 715, 1307, 1400
NciI CCSGG 1 cut(s) 1262
NdeI CATATG 1 cut(s) 435
NdeII GATC 5 cut(s) 172, 408, 494, 559, 1347
NlaIV GGNNCC 6 cut(s) 174, 859, 1123, 1156, 1236, 1258
NmuCI GTSAC 2 cut(s) 817, 837
NspI RCATGY 2 cut(s) 91, 444
NspV TTCGAA 1 cut(s) 636
PagI TCATGA 3 cut(s) 288, 447, 538
PciI ACATGT 1 cut(s) 87
PctI GAATGC 2 cut(s) 157, 774
PdmI GAANNNNTTC 2 cut(s) 393, 1382
PfeI GAWTC 2 cut(s) 362, 546
PflMI CCANNNNNTGG 2 cut(s) 216, 1087
PfoI TCCNGGA 2 cut(s) 955, 1213
PkrI GCNGC 5 cut(s) 406, 455, 1195, 1327, 1393
PscI ACATGT 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 725
Psp6I CCWGG 9 cut(s) 124, 209, 316, 510, 778, 955, 1157, 1213, 1237
PspGI CCWGG 9 cut(s) 124, 209, 316, 510, 778, 955, 1157, 1213, 1237
PspN4I GGNNCC 6 cut(s) 174, 859, 1123, 1156, 1236, 1258
PspPI GGNCC 2 cut(s) 848, 971
PsuI RGATCY 2 cut(s) 172, 494
RsaI GTAC 3 cut(s) 920, 1034, 1502
RsaNI GTAC 3 cut(s) 919, 1033, 1501
RseI CAYNNNNRTG 2 cut(s) 353, 1250
SaqAI TTAA 2 cut(s) 530, 725
SatI GCNGC 5 cut(s) 405, 454, 1194, 1326, 1392
Sau3AI GATC 5 cut(s) 172, 408, 494, 559, 1347
Sau96I GGNCC 2 cut(s) 848, 971
SduI GDGCHC 2 cut(s) 201, 668
SfaNI GCATC 2 cut(s) 1254, 1312
SfuI TTCGAA 1 cut(s) 636
SmiMI CAYNNNNRTG 2 cut(s) 353, 1250
SmlI CTYRAG 1 cut(s) 1069
SmoI CTYRAG 1 cut(s) 1069
SsiI CCGC 7 cut(s) 185, 404, 772, 1119, 1193, 1392, 1435
SspMI CTAG 4 cut(s) 324, 480, 741, 1275
TaaI ACNGT 3 cut(s) 590, 1154, 1210
TaiI ACGT 1 cut(s) 757
TaqI TCGA 3 cut(s) 636, 801, 1164
TaqII GACCGA 1 cut(s) 247
TatI WGTACW 2 cut(s) 1032, 1500
TauI GCSGC 3 cut(s) 407, 1196, 1394
TfiI GAWTC 2 cut(s) 362, 546
Tru1I TTAA 2 cut(s) 530, 725
Tru9I TTAA 2 cut(s) 530, 725
TscAI CASTG 5 cut(s) 165, 526, 673, 846, 1213
TseFI GTSAC 2 cut(s) 817, 837
TseI GCWGC 2 cut(s) 453, 1325
Tsp45I GTSAC 2 cut(s) 817, 837
TspDTI ATGAA 4 cut(s) 120, 337, 422, 1119
TspRI CASTG 5 cut(s) 165, 526, 673, 846, 1213
Van91I CCANNNNNTGG 2 cut(s) 216, 1087
VspI ATTAAT 1 cut(s) 725
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 1 cut(s) 1333
XbaI TCTAGA 1 cut(s) 479
XceI RCATGY 2 cut(s) 91, 444
XcmI CCANNNNNNNNNTGG 1 cut(s) 989
XmnI GAANNNNTTC 2 cut(s) 393, 1382
XspI CTAG 4 cut(s) 324, 480, 741, 1275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.