RchiOBHm_Chr2g0110071

Serine hydroxymethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007608
Physical Location & Seq
Reverse (-)
21435436 .. 21441482
6047 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48381

Sequence Viewer

Length: 1554 bp
ATGGCACTGGCATTGGCCCTTCGCAGGCTTTCCTCTTCTGTGGACAAGCCCATTCGTCCTCTCTTCAATGCCACGTCTCACTATTACATGTCATCTTTGCCCAATGAAGCTGTCTATGAGAAGGAGAAGAACGGTGTCAGTTGGCCTAAGCAACTGAATGCTCCATTGGAGGCTGTGGATCCTGAGATTGCTGACATCATTGAGCACGAGAAAGCCAGGCAATGGAAGGGCCTAGAACTGATACCTTCAGAGAACTTCACATCAGTCTCTGTGATGCAAGCAGTTGGGTCAGTCATGACCAACAAATACAGTGAAGGGTATCCTGGGGCAAGATACTATGGAGGAAATGAGTTCATTGACATGGCAGAAACCTTGTGCCAGAAACGTGCTTTGGAAGCTTTTCGGTTGGACCCAGCAAAATGGGGAGTCAACGTGCAGCCTTTGTCAGGATCTCCAGCTAATTTTCATGTTTACACTGCATTGTTAAAACCTCATGATAGAATCATGGCTCTTGATCTTCCTCATGGTGGTCATCTTTCTCATGGTTATCAGACTGACACCAAAAAGATATCAGCTGTGTCAATCTTTTTTGAGACAATGCCATACAGATTGGATGAGAGCAGTGGTTACATTGACTACGACCAGTTGGAGAAAAGTGCCACTCTCTTCAGGCCAAAATTAATAGTTGCTGGTGCTAGTGCTTATGCACGTTTGTATGATTACGCACGCATTCGCAAGGTATGCGACAAACAAAAAGCTATCCTGTTGGCAGATATGGCACATATCAGTGGGTTGGTTGCAGCTGGTGTCATCCCATCTCCTTTTGAGTATGCAGATGTAGTGACCACCACAACACACAAGTCACTTCGTGGCCCTCGTGGTGCCATGATTTTCTTCAGGAAGGGTCTAAAAGAGACTAACAAACAAGGGAAAGAGGTGTTTTATGACTACGAAGACAAAATCAATCAAGCTGTCTTTCCTGGGCTTCAAGGTGGTCCACACAACCACACCATCACTGGTTTGGCAGTTGCACTGAAACAGGCTACTACTCCAGAGTACAAAGCCTATCAAGAGCAAGTTCTCAGTAATTGCTCAAGATTTGCACAGTCATTGAATGAGAAGGGCTATGATCTTGTTTCTGGCGGAACTGAGAACCATTTAGTTTTGGTGAATTTGAAGAACAAGGGCATTGACGGCTCCAGGGTTGAAAAGGTGTTGGAAGCTGTTCACATTGCAGCCAACAAAAACACTGTTCCTGGAGATGTGTCTGCCATGGTTCCTGGTGGCATCAGGATGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTCGCTAAAGTTGCAGATTTTTTTGATGCTGCTGTGAAGTTGGCAGTGAAGGTCAAGGGGGAAGCCAAAGGAACGAAGTTGAAGGACTTCTTGGCAGCACTGCCTGCCCCTCACTTTCAGTCTGAAATTGCAAAGCTCCGCCATGATGTCGAGGAGTATGCCAAGCAATTTCCTACAATTGGATTTGAGAAGGAAACGATGAAGTACAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001505 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004372 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0005886 GO:0006082 GO:0006520 GO:0006544 GO:0006563 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008150 GO:0008152 GO:0008187 GO:0008219 GO:0008266 GO:0009069 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009626 GO:0009628 GO:0009853 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0012501 GO:0016020 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019752 GO:0022626 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0033554 GO:0034050 GO:0042133 GO:0042221 GO:0043094 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045087 GO:0046686 GO:0048046 GO:0048511 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901605 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523
Pfam Domains
Protein Families
SHMT

Protein Analysis

517

Amino Acids

57.15

Weight (kDa)

8.56

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 56 - 453 3.7e-214 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 881
AccB7I CCANNNNNTGG 1 cut(s) 222
AciI CCGC 2 cut(s) 1143, 1477
AclWI GGATC 3 cut(s) 173, 186, 457
AcsI RAATTY 1 cut(s) 1171
AcuI CTGAAG 3 cut(s) 231, 652, 880
AdeI CACNNNGTG 1 cut(s) 869
AfaI GTAC 2 cut(s) 1058, 1544
AfiI CCNNNNNNNGG 5 cut(s) 24, 222, 446, 527, 1517
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 6 cut(s) 67, 989, 1114, 1177, 1208, 1420
AjiI CACGTC 1 cut(s) 75
AjnI CCWGG 6 cut(s) 215, 322, 979, 1199, 1255, 1279
AjuI GAANNNNNNNTTGG 6 cut(s) 149, 181, 374, 406, 1412, 1444
AluBI AGCT 9 cut(s) 110, 398, 458, 575, 758, 803, 971, 1223, 1474
AluI AGCT 9 cut(s) 110, 398, 458, 575, 758, 803, 971, 1223, 1474
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 4 cut(s) 81, 271, 587, 908
AlwI GGATC 3 cut(s) 173, 186, 457
AlwNI CAGNNNCTG 1 cut(s) 269
AoxI GGCC 5 cut(s) 15, 143, 229, 671, 871
ApeKI GCWGC 5 cut(s) 436, 800, 1235, 1367, 1433
ApoI RAATTY 1 cut(s) 1171
AseI ATTAAT 1 cut(s) 680
Asp700I GAANNNNTTC 3 cut(s) 399, 1224, 1424
AspS9I GGNCC 5 cut(s) 16, 229, 409, 872, 995
AsuHPI GGTGA 1 cut(s) 1180
AvaII GGWCC 2 cut(s) 409, 995
BamHI GGATCC 1 cut(s) 178
BanI GGYRCC 1 cut(s) 881
BauI CACGAG 2 cut(s) 206, 876
BbsI GAAGAC 1 cut(s) 960
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 5 cut(s) 448, 812, 1247, 1354, 1445
BccI CCATC 3 cut(s) 823, 1019, 1288
BceAI ACGGC 1 cut(s) 1210
BcgI CGANNNNNNTGC 2 cut(s) 1478, 1512
BciT130I CCWGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
BciVI GTATCC 1 cut(s) 330
BcoDI GTCTC 4 cut(s) 81, 271, 587, 908
BfaI CTAG 3 cut(s) 233, 696, 1317
BfuI GTATCC 1 cut(s) 330
BisI GCNGC 5 cut(s) 437, 801, 1236, 1368, 1434
BlsI GCNGC 5 cut(s) 438, 802, 1237, 1369, 1435
Bme1390I CCNGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
Bme18I GGWCC 2 cut(s) 409, 995
BmgBI CACGTC 1 cut(s) 75
BmgT120I GGNCC 5 cut(s) 16, 229, 409, 872, 995
BmiI GGNNCC 5 cut(s) 180, 411, 883, 1198, 1278
BmrFI CCNGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
BmsI GCATC 3 cut(s) 264, 1296, 1354
BpiI GAAGAC 1 cut(s) 960
BpmI CTGGAG 4 cut(s) 438, 1035, 1183, 1278
Bpu10I CCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 1078
BsaJI CCNNGG 4 cut(s) 323, 980, 1200, 1272
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 5 cut(s) 24, 222, 446, 527, 1517
Bse1I ACTGG 3 cut(s) 12, 643, 1021
Bse3DI GCAATG 2 cut(s) 227, 1230
BseBI CCWGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
BseDI CCNNGG 4 cut(s) 323, 980, 1200, 1272
BseGI GGATG 3 cut(s) 619, 810, 1299
BseLI CCNNNNNNNGG 5 cut(s) 24, 222, 446, 527, 1517
BseMI GCAATG 2 cut(s) 227, 1230
BseMII CTCAG 3 cut(s) 174, 1096, 1140
BseNI ACTGG 3 cut(s) 12, 643, 1021
BseRI GAGGAG 2 cut(s) 1346, 1505
BseXI GCAGC 5 cut(s) 448, 812, 1247, 1354, 1445
BseYI CCCAGC 1 cut(s) 412
BsgI GTGCAG 1 cut(s) 455
BshFI GGCC 5 cut(s) 17, 145, 231, 673, 873
BshNI GGYRCC 1 cut(s) 881
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 1304
BslI CCNNNNNNNGG 5 cut(s) 24, 222, 446, 527, 1517
BsmAI GTCTC 4 cut(s) 81, 271, 587, 908
BsmBI CGTCTC 1 cut(s) 81
BsmI GAATGC 2 cut(s) 163, 729
BsnI GGCC 5 cut(s) 17, 145, 231, 673, 873
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 4 cut(s) 178, 449, 514, 1129
Bsp19I CCATGG 1 cut(s) 1272
BspACI CCGC 2 cut(s) 1143, 1477
BspANI GGCC 5 cut(s) 17, 145, 231, 673, 873
BspCNI CTCAG 3 cut(s) 175, 1095, 1141
BspHI TCATGA 2 cut(s) 294, 493
BspLI GGNNCC 5 cut(s) 180, 411, 883, 1198, 1278
BspPI GGATC 3 cut(s) 173, 186, 457
BspT107I GGYRCC 1 cut(s) 881
BsrDI GCAATG 2 cut(s) 227, 1230
BsrI ACTGG 3 cut(s) 12, 643, 1021
BssECI CCNNGG 4 cut(s) 323, 980, 1200, 1272
BssMI GATC 4 cut(s) 178, 449, 514, 1129
BssSI CACGAG 2 cut(s) 206, 876
BssT1I CCWWGG 1 cut(s) 1272
Bst2BI CACGAG 2 cut(s) 206, 876
Bst2UI CCWGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
Bst4CI ACNGT 4 cut(s) 134, 311, 1107, 1252
Bst6I CTCTTC 3 cut(s) 40, 68, 671
BstAPI GCANNNNNTGC 2 cut(s) 741, 1442
BstC8I GCNNGC 4 cut(s) 26, 279, 727, 1443
BstDEI CTNAG 4 cut(s) 147, 183, 1082, 1149
BstDSI CCRYGG 1 cut(s) 1272
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 3 cut(s) 619, 810, 1299
BstKTI GATC 4 cut(s) 181, 452, 517, 1132
BstMAI GTCTC 4 cut(s) 81, 271, 587, 908
BstMBI GATC 4 cut(s) 178, 449, 514, 1129
BstMWI GCNNNNNNNGC 6 cut(s) 395, 741, 776, 1194, 1349, 1442
BstNI CCWGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 6 cut(s) 215, 322, 979, 1199, 1255, 1279
BstV1I GCAGC 5 cut(s) 448, 812, 1247, 1354, 1445
BstV2I GAAGAC 1 cut(s) 960
BstX2I RGATCY 2 cut(s) 178, 449
BstXI CCANNNNNNTGG 1 cut(s) 420
BstYI RGATCY 2 cut(s) 178, 449
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 5 cut(s) 17, 145, 231, 673, 873
BtgI CCRYGG 1 cut(s) 1272
BtrI CACGTC 1 cut(s) 75
BtsCI GGATG 3 cut(s) 619, 810, 1299
BtsI GCAGTG 4 cut(s) 474, 628, 1389, 1436
Cac8I GCNNGC 4 cut(s) 26, 279, 727, 1443
CaiI CAGNNNCTG 1 cut(s) 269
CciI TCATGA 2 cut(s) 294, 493
Cfr13I GGNCC 5 cut(s) 16, 229, 409, 872, 995
Csp6I GTAC 2 cut(s) 1057, 1543
CviQI GTAC 2 cut(s) 1057, 1543
DdeI CTNAG 4 cut(s) 147, 183, 1082, 1149
DpnI GATC 4 cut(s) 180, 451, 516, 1131
DpnII GATC 4 cut(s) 178, 449, 514, 1129
DraIII CACNNNGTG 1 cut(s) 869
Eam1104I CTCTTC 3 cut(s) 40, 68, 671
EarI CTCTTC 3 cut(s) 40, 68, 671
EciI GGCGGA 2 cut(s) 1158, 1466
Eco130I CCWWGG 1 cut(s) 1272
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 2 cut(s) 409, 995
Eco57I CTGAAG 3 cut(s) 231, 652, 880
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 6 cut(s) 215, 322, 979, 1199, 1255, 1279
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 1 cut(s) 1272
ErhI CCWWGG 1 cut(s) 1272
Esp3I CGTCTC 1 cut(s) 81
FalI AAGNNNNNCTT 2 cut(s) 1412, 1444
Fnu4HI GCNGC 5 cut(s) 437, 801, 1236, 1368, 1434
FokI GGATG 3 cut(s) 626, 797, 1306
Fsp4HI GCNGC 5 cut(s) 437, 801, 1236, 1368, 1434
FspBI CTAG 3 cut(s) 233, 696, 1317
GluI GCNGC 5 cut(s) 437, 801, 1236, 1368, 1434
GsaI CCCAGC 1 cut(s) 416
GsuI CTGGAG 4 cut(s) 438, 1035, 1183, 1278
HaeIII GGCC 5 cut(s) 17, 145, 231, 673, 873
HapII CCGG 1 cut(s) 1304
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 2 cut(s) 426, 501
HpaII CCGG 1 cut(s) 1304
HphI GGTGA 1 cut(s) 1180
Hpy166II GTNNAC 5 cut(s) 43, 430, 472, 998, 1228
Hpy188I TCNGA 3 cut(s) 250, 552, 1462
Hpy8I GTNNAC 5 cut(s) 43, 430, 472, 998, 1228
HpyCH4III ACNGT 4 cut(s) 134, 311, 1107, 1252
HpyCH4IV ACGT 4 cut(s) 74, 385, 432, 709
HpyF10VI GCNNNNNNNGC 6 cut(s) 395, 741, 776, 1194, 1349, 1442
HpyF3I CTNAG 4 cut(s) 147, 183, 1082, 1149
HpySE526I ACGT 4 cut(s) 74, 385, 432, 709
Kzo9I GATC 4 cut(s) 178, 449, 514, 1129
LmnI GCTCC 3 cut(s) 166, 1202, 1479
Lsp1109I GCAGC 5 cut(s) 448, 812, 1247, 1354, 1445
LweI GCATC 3 cut(s) 264, 1296, 1354
MaeI CTAG 3 cut(s) 233, 696, 1317
MaeII ACGT 4 cut(s) 74, 385, 432, 709
MaeIII GTNAC 3 cut(s) 626, 841, 861
MalI GATC 4 cut(s) 180, 451, 516, 1131
MboI GATC 4 cut(s) 178, 449, 514, 1129
MboII GAAGA 8 cut(s) 27, 55, 139, 509, 658, 886, 965, 1189
MfeI CAATTG 1 cut(s) 1515
MflI RGATCY 2 cut(s) 178, 449
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 7 cut(s) 460, 677, 1087, 1171, 1464, 1505, 1515
MlyI GAGTC 1 cut(s) 435
MmeI TCCRAC 3 cut(s) 387, 627, 1197
MroXI GAANNNNTTC 3 cut(s) 399, 1224, 1424
MseI TTAA 2 cut(s) 485, 680
MslI CAYNNNNRTG 3 cut(s) 359, 786, 1292
MspA1I CMGCKG 2 cut(s) 575, 803
MspI CCGG 1 cut(s) 1304
MspR9I CCNGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
MunI CAATTG 1 cut(s) 1515
Mva1269I GAATGC 2 cut(s) 163, 729
MvaI CCWGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
MwoI GCNNNNNNNGC 6 cut(s) 395, 741, 776, 1194, 1349, 1442
NcoI CCATGG 1 cut(s) 1272
NdeII GATC 4 cut(s) 178, 449, 514, 1129
NlaIV GGNNCC 5 cut(s) 180, 411, 883, 1198, 1278
NmuCI GTSAC 2 cut(s) 841, 861
NspI RCATGY 1 cut(s) 91
PagI TCATGA 2 cut(s) 294, 493
PciI ACATGT 1 cut(s) 87
PcsI WCGNNNNNNNCGW 1 cut(s) 874
PctI GAATGC 2 cut(s) 163, 729
PdmI GAANNNNTTC 3 cut(s) 399, 1224, 1424
PfeI GAWTC 1 cut(s) 501
PflMI CCANNNNNTGG 1 cut(s) 222
PfoI TCCNGGA 1 cut(s) 1255
PkrI GCNGC 5 cut(s) 438, 802, 1237, 1369, 1435
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
PscI ACATGT 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 680
Psp6I CCWGG 6 cut(s) 215, 322, 979, 1199, 1255, 1279
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 6 cut(s) 215, 322, 979, 1199, 1255, 1279
PspN4I GGNNCC 5 cut(s) 180, 411, 883, 1198, 1278
PspPI GGNCC 5 cut(s) 16, 229, 409, 872, 995
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 2 cut(s) 178, 449
PvuII CAGCTG 2 cut(s) 575, 803
RsaI GTAC 2 cut(s) 1058, 1544
RsaNI GTAC 2 cut(s) 1057, 1543
RseI CAYNNNNRTG 3 cut(s) 359, 786, 1292
SaqAI TTAA 2 cut(s) 485, 680
SatI GCNGC 5 cut(s) 437, 801, 1236, 1368, 1434
Sau3AI GATC 4 cut(s) 178, 449, 514, 1129
Sau96I GGNCC 5 cut(s) 16, 229, 409, 872, 995
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 6 cut(s) 217, 324, 981, 1201, 1257, 1281
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 3 cut(s) 264, 1296, 1354
SinI GGWCC 2 cut(s) 409, 995
SmiMI CAYNNNNRTG 3 cut(s) 359, 786, 1292
SmlI CTYRAG 1 cut(s) 1093
SmoI CTYRAG 1 cut(s) 1093
Sse9I AATT 7 cut(s) 460, 677, 1087, 1171, 1464, 1505, 1515
SsiI CCGC 2 cut(s) 1143, 1477
SspMI CTAG 3 cut(s) 233, 696, 1317
StyD4I CCNGG 6 cut(s) 215, 322, 979, 1199, 1255, 1279
StyI CCWWGG 1 cut(s) 1272
TaaI ACNGT 4 cut(s) 134, 311, 1107, 1252
TaiI ACGT 4 cut(s) 77, 388, 435, 712
TaqI TCGA 1 cut(s) 1488
TasI AATT 7 cut(s) 460, 677, 1087, 1171, 1464, 1505, 1515
TatI WGTACW 2 cut(s) 1056, 1542
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 2 cut(s) 485, 680
Tru9I TTAA 2 cut(s) 485, 680
TseFI GTSAC 2 cut(s) 841, 861
TseI GCWGC 5 cut(s) 436, 800, 1235, 1367, 1433
Tsp45I GTSAC 2 cut(s) 841, 861
TspDTI ATGAA 4 cut(s) 120, 343, 455, 1553
Van91I CCANNNNNTGG 1 cut(s) 222
VpaK11BI GGWCC 2 cut(s) 409, 995
VspI ATTAAT 1 cut(s) 680
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 1 cut(s) 1171
XceI RCATGY 1 cut(s) 91
XcmI CCANNNNNNNNNTGG 2 cut(s) 1013, 1018
XmnI GAANNNNTTC 3 cut(s) 399, 1224, 1424
XspI CTAG 3 cut(s) 233, 696, 1317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.