Rorug02G0165500

Serine hydroxymethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007608
Physical Location & Seq
Forward (+)
14756735 .. 14758334
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0165500.1

Sequence Viewer

Length: 978 bp
ATGATCCAAGAACTGTTCGGAGGCGCAGGCTTTAATACAGGAGGAGTAGGAGGAGGAGGAGAGAGGAAAATCTCCATTTCTTCTTCTTCTACTTCTCTTTCGCCTTCGCCGTCTCCTTCTTCTTCCACTACTACTACCACCACCGCCGCGGCCGCTGCAACAGCGAATTCAGAGAACTTGAGATGCCCGAGATGCGATTCTGCCAACACCAAGTTCTGTTACTACAACAACTACAACCTCACACAGCCCCGCCACTTCTGCAAGACTTGCCGCCGCTACTGGACCAAGGGAGGAGCCCTAAGGAATGTTCCGATTGGGGGTGGATGCCGGAAAAACAAGAATGGTACCATGTCAACATCGATAAGCAAGACAGCTGTGGCCGGGAAGTTGAAAACAATGGCGTCGGAGATCGGGAGGTCCGGGTTCGGACTCGGGTTTGATCACGAAGTGCAAGCAAGTCCGATACTGTGGGGTTCACCTCAGAATTCCCATATCCTAGCTCTACTAAGATCCAGCACTCCAAACCCTAACCCTAACCCTAATGTTCAACTGTGTACTAATTCGGTGAAGGAAGAGGGGGGCATGAATATGATTGGGTCCCACATGATGAGTGAGTCTTCTGGAGCTGGAAATGATGCAATGACAGCTCGGACCTTGGGGTTGGATCCGCTGAGTTCTTTCTGGAGAAACAACCAGAACGTTCAACCTCCACACCACCACCACCAACAGAATGGTAATTTCTTACTTGGGCATGAGGTTCAAAGCAACCAAGGAATTCAGGAACTATTTCAGAGGCTTAGATCATCCTCTTCATCAGGTAGTTATTATTCGCATGATCTTAATAATGGGGTTTCTTCATCTTCTACATCATCGATTTTGGAGGCGGCTCCGGCTGTTGGTGGTGAATTGGGTTATAACTGGAACCCAGCGTTTACTTGGTCCGATCAGCTCCCTACAACAAATGGTGCATACCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001505 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004372 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0005886 GO:0006082 GO:0006520 GO:0006544 GO:0006563 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008150 GO:0008152 GO:0008187 GO:0008219 GO:0008266 GO:0009069 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009626 GO:0009628 GO:0009853 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0012501 GO:0016020 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019752 GO:0022626 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0033554 GO:0034050 GO:0042133 GO:0042221 GO:0043094 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045087 GO:0046686 GO:0048046 GO:0048511 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901605 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523
Pfam Domains
Protein Families
SHMT

Protein Analysis

325

Amino Acids

34.51

Weight (kDa)

8.98

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 59 - 114 5.8e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
Acc65I GGTACC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 344
AccII CGCG 1 cut(s) 149
AciI CCGC 9 cut(s) 144, 147, 149, 153, 250, 271, 274, 668, 884
AclI AACGTT 1 cut(s) 699
AclWI GGATC 3 cut(s) 504, 659, 672
AcoI YGGCCR 2 cut(s) 150, 378
AcsI RAATTY 3 cut(s) 166, 484, 774
AcyI GRCGYC 1 cut(s) 401
AdeI CACNNNGTG 1 cut(s) 448
AfaI GTAC 2 cut(s) 346, 556
AfiI CCNNNNNNNGG 2 cut(s) 317, 896
AgsI TTSAA 4 cut(s) 391, 548, 704, 761
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 5 cut(s) 374, 500, 626, 647, 949
AluI AGCT 5 cut(s) 374, 500, 626, 647, 949
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 3 cut(s) 504, 659, 672
Ama87I CYCGRG 2 cut(s) 187, 431
AoxI GGCC 2 cut(s) 150, 378
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 3 cut(s) 166, 484, 774
Asp700I GAANNNNTTC 1 cut(s) 786
Asp718I GGTACC 1 cut(s) 344
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 5 cut(s) 282, 417, 597, 651, 939
AsuC2I CCSGG 2 cut(s) 382, 421
AsuHPI GGTGA 3 cut(s) 468, 577, 914
AvaI CYCGRG 2 cut(s) 187, 431
AvaII GGWCC 5 cut(s) 282, 417, 597, 651, 939
AxyI CCTNAGG 1 cut(s) 299
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 609
BbvI GCAGC 1 cut(s) 142
BceAI ACGGC 1 cut(s) 94
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 2 cut(s) 382, 421
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 1 cut(s) 497
BisI GCNGC 7 cut(s) 147, 150, 153, 156, 271, 274, 885
BlsI GCNGC 7 cut(s) 148, 151, 154, 157, 272, 275, 886
Bme1390I CCNGG 2 cut(s) 382, 421
Bme18I GGWCC 5 cut(s) 282, 417, 597, 651, 939
BmeT110I CYCGRG 2 cut(s) 187, 431
BmgT120I GGNCC 5 cut(s) 282, 417, 597, 651, 939
BmiI GGNNCC 7 cut(s) 295, 346, 598, 599, 666, 888, 923
BmrFI CCNGG 2 cut(s) 382, 421
BmsI GCATC 4 cut(s) 173, 182, 314, 625
BpiI GAAGAC 1 cut(s) 609
BpmI CTGGAG 2 cut(s) 642, 703
BpuEI CTTGAG 1 cut(s) 199
BpuMI CCSGG 2 cut(s) 382, 421
Bsa29I ATCGAT 2 cut(s) 359, 872
BsaHI GRCGYC 1 cut(s) 401
BsaJI CCNNGG 4 cut(s) 147, 285, 654, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 896
Bse1I ACTGG 2 cut(s) 284, 923
Bse21I CCTNAGG 1 cut(s) 299
Bse3DI GCAATG 1 cut(s) 645
BseCI ATCGAT 2 cut(s) 359, 872
BseDI CCNNGG 4 cut(s) 147, 285, 654, 769
BseGI GGATG 2 cut(s) 329, 803
BseLI CCNNNNNNNGG 2 cut(s) 317, 896
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 2 cut(s) 494, 662
BseNI ACTGG 2 cut(s) 284, 923
BseRI GAGGAG 5 cut(s) 57, 66, 69, 72, 306
BseX3I CGGCCG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 142
BseYI CCCAGC 1 cut(s) 925
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 152, 380
BshNI GGYRCC 1 cut(s) 344
BshVI ATCGAT 2 cut(s) 359, 872
BsiEI CGRYCG 1 cut(s) 153
BsiHKCI CYCGRG 2 cut(s) 187, 431
BsiSI CCGG 4 cut(s) 328, 381, 420, 890
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 2 cut(s) 317, 896
BsmAI GTCTC 1 cut(s) 117
BsmBI CGTCTC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 583
BsnI GGCC 2 cut(s) 152, 380
BsoBI CYCGRG 2 cut(s) 187, 431
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 8 cut(s) 3, 408, 439, 509, 664, 800, 835, 943
BspACI CCGC 9 cut(s) 144, 147, 149, 153, 250, 271, 274, 668, 884
BspANI GGCC 2 cut(s) 152, 380
BspCNI CTCAG 2 cut(s) 493, 663
BspDI ATCGAT 2 cut(s) 359, 872
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 7 cut(s) 295, 346, 598, 599, 666, 888, 923
BspPI GGATC 3 cut(s) 504, 659, 672
BspT107I GGYRCC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 645
BsrI ACTGG 2 cut(s) 284, 923
BssECI CCNNGG 4 cut(s) 147, 285, 654, 769
BssMI GATC 8 cut(s) 3, 408, 439, 509, 664, 800, 835, 943
BssNI GRCGYC 1 cut(s) 401
BssT1I CCWWGG 3 cut(s) 285, 654, 769
Bst4CI ACNGT 3 cut(s) 15, 468, 552
Bst6I CTCTTC 2 cut(s) 567, 814
BstACI GRCGYC 1 cut(s) 401
BstAPI GCANNNNNTGC 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 28, 453
BstDEI CTNAG 5 cut(s) 299, 480, 506, 671, 797
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 2 cut(s) 329, 803
BstFNI CGCG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 8 cut(s) 6, 411, 442, 512, 667, 803, 838, 946
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 8 cut(s) 3, 408, 439, 509, 664, 800, 835, 943
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 8 cut(s) 152, 155, 161, 192, 258, 267, 644, 890
BstSCI CCNGG 2 cut(s) 380, 419
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 1 cut(s) 609
BstX2I RGATCY 2 cut(s) 509, 664
BstXI CCANNNNNNTGG 1 cut(s) 731
BstYI RGATCY 2 cut(s) 509, 664
BstZI CGGCCG 1 cut(s) 150
Bsu15I ATCGAT 2 cut(s) 359, 872
Bsu36I CCTNAGG 1 cut(s) 299
BsuRI GGCC 2 cut(s) 152, 380
BsuTUI ATCGAT 2 cut(s) 359, 872
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 2 cut(s) 329, 803
Cac8I GCNNGC 2 cut(s) 28, 453
CciNI GCGGCCGC 1 cut(s) 150
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 5 cut(s) 282, 417, 597, 651, 939
Cfr42I CCGCGG 1 cut(s) 150
ClaI ATCGAT 2 cut(s) 359, 872
CseI GACGC 1 cut(s) 390
Csp6I GTAC 2 cut(s) 345, 555
CviAII CATG 5 cut(s) 349, 583, 604, 752, 833
CviQI GTAC 2 cut(s) 345, 555
DdeI CTNAG 5 cut(s) 299, 480, 506, 671, 797
DpnI GATC 8 cut(s) 5, 410, 441, 511, 666, 802, 837, 945
DpnII GATC 8 cut(s) 3, 408, 439, 509, 664, 800, 835, 943
DraIII CACNNNGTG 1 cut(s) 448
EaeI YGGCCR 2 cut(s) 150, 378
EagI CGGCCG 1 cut(s) 150
Eam1104I CTCTTC 2 cut(s) 567, 814
EarI CTCTTC 2 cut(s) 567, 814
EclXI CGGCCG 1 cut(s) 150
Eco130I CCWWGG 3 cut(s) 285, 654, 769
Eco24I GRGCYC 1 cut(s) 298
Eco47I GGWCC 5 cut(s) 282, 417, 597, 651, 939
Eco52I CGGCCG 1 cut(s) 150
Eco81I CCTNAGG 1 cut(s) 299
Eco88I CYCGRG 2 cut(s) 187, 431
EcoO109I RGGNCCY 1 cut(s) 597
EcoRI GAATTC 3 cut(s) 166, 484, 774
EcoT14I CCWWGG 3 cut(s) 285, 654, 769
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 3 cut(s) 285, 654, 769
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 5 cut(s) 352, 586, 607, 755, 836
FaiI YATR 9 cut(s) 350, 492, 584, 590, 605, 753, 834, 915, 970
FaqI GGGAC 1 cut(s) 583
FatI CATG 5 cut(s) 348, 582, 603, 751, 832
FauI CCCGC 1 cut(s) 257
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 7 cut(s) 147, 150, 153, 156, 271, 274, 885
FokI GGATG 2 cut(s) 336, 790
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 7 cut(s) 147, 150, 153, 156, 271, 274, 885
FspBI CTAG 1 cut(s) 497
GlaI GCGC 1 cut(s) 25
GluI GCNGC 7 cut(s) 147, 150, 153, 156, 271, 274, 885
GsaI CCCAGC 1 cut(s) 929
GsuI CTGGAG 2 cut(s) 642, 703
HaeIII GGCC 2 cut(s) 152, 380
HapII CCGG 4 cut(s) 328, 381, 420, 890
HgaI GACGC 1 cut(s) 390
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 5 cut(s) 352, 586, 607, 755, 836
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 3 cut(s) 197, 429, 614
HpaII CCGG 4 cut(s) 328, 381, 420, 890
HphI GGTGA 3 cut(s) 468, 577, 914
Hpy166II GTNNAC 4 cut(s) 354, 476, 555, 933
Hpy188III TCNNGA 5 cut(s) 412, 443, 621, 682, 779
Hpy8I GTNNAC 4 cut(s) 354, 476, 555, 933
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 3 cut(s) 114, 126, 562
HpyCH4III ACNGT 3 cut(s) 15, 468, 552
HpyCH4IV ACGT 1 cut(s) 699
HpyCH4V TGCA 5 cut(s) 158, 261, 451, 638, 968
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 155, 161, 192, 258, 267, 644, 890
HpyF3I CTNAG 5 cut(s) 299, 480, 506, 671, 797
HpySE526I ACGT 1 cut(s) 699
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 5 cut(s) 352, 586, 607, 755, 836
HspAI GCGC 1 cut(s) 24
KflI GGGWCCC 1 cut(s) 597
KpnI GGTACC 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 439
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 8 cut(s) 3, 408, 439, 509, 664, 800, 835, 943
LmnI GCTCC 4 cut(s) 293, 623, 892, 954
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 4 cut(s) 173, 182, 314, 625
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 1 cut(s) 218
MalI GATC 8 cut(s) 5, 410, 441, 511, 666, 802, 837, 945
MboI GATC 8 cut(s) 3, 408, 439, 509, 664, 800, 835, 943
MflI RGATCY 2 cut(s) 509, 664
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 6 cut(s) 166, 484, 559, 736, 774, 905
MlyI GAGTC 2 cut(s) 423, 623
MmeI TCCRAC 2 cut(s) 384, 642
MroXI GAANNNNTTC 1 cut(s) 786
MseI TTAA 3 cut(s) 33, 840, 976
MslI CAYNNNNRTG 1 cut(s) 587
MspA1I CMGCKG 4 cut(s) 149, 155, 374, 670
MspI CCGG 4 cut(s) 328, 381, 420, 890
MspR9I CCNGG 2 cut(s) 382, 421
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 8 cut(s) 152, 155, 161, 192, 258, 267, 644, 890
NciI CCSGG 2 cut(s) 382, 421
NdeII GATC 8 cut(s) 3, 408, 439, 509, 664, 800, 835, 943
NlaIII CATG 5 cut(s) 352, 586, 607, 755, 836
NlaIV GGNNCC 7 cut(s) 295, 346, 598, 599, 666, 888, 923
NotI GCGGCCGC 1 cut(s) 150
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 7 cut(s) 148, 151, 154, 157, 272, 275, 886
PleI GAGTC 2 cut(s) 423, 622
PpsI GAGTC 2 cut(s) 423, 622
PpuMI RGGWCCY 1 cut(s) 597
PsiI TTATAA 1 cut(s) 915
Psp1406I AACGTT 1 cut(s) 699
Psp5II RGGWCCY 1 cut(s) 597
PspFI CCCAGC 1 cut(s) 925
PspN4I GGNNCC 7 cut(s) 295, 346, 598, 599, 666, 888, 923
PspPI GGNCC 5 cut(s) 282, 417, 597, 651, 939
PspPPI RGGWCCY 1 cut(s) 597
PsuI RGATCY 2 cut(s) 509, 664
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 2 cut(s) 346, 556
RsaNI GTAC 2 cut(s) 345, 555
RseI CAYNNNNRTG 1 cut(s) 587
SacII CCGCGG 1 cut(s) 150
SaqAI TTAA 3 cut(s) 33, 840, 976
SatI GCNGC 7 cut(s) 147, 150, 153, 156, 271, 274, 885
Sau3AI GATC 8 cut(s) 3, 408, 439, 509, 664, 800, 835, 943
Sau96I GGNCC 5 cut(s) 282, 417, 597, 651, 939
SchI GAGTC 2 cut(s) 423, 623
ScrFI CCNGG 2 cut(s) 382, 421
SduI GDGCHC 1 cut(s) 298
SfaNI GCATC 4 cut(s) 173, 182, 314, 625
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 5 cut(s) 282, 417, 597, 651, 939
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 6 cut(s) 166, 484, 559, 736, 774, 905
SsiI CCGC 9 cut(s) 144, 147, 149, 153, 250, 271, 274, 668, 884
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 2 cut(s) 380, 419
StyI CCWWGG 3 cut(s) 285, 654, 769
TaaI ACNGT 3 cut(s) 15, 468, 552
TaiI ACGT 1 cut(s) 702
TaqI TCGA 2 cut(s) 359, 872
TasI AATT 6 cut(s) 166, 484, 559, 736, 774, 905
TatI WGTACW 1 cut(s) 554
TauI GCSGC 6 cut(s) 149, 152, 155, 273, 276, 887
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 3 cut(s) 33, 840, 976
Tru9I TTAA 3 cut(s) 33, 840, 976
TseI GCWGC 1 cut(s) 155
TspDTI ATGAA 3 cut(s) 599, 801, 846
VpaK11BI GGWCC 5 cut(s) 282, 417, 597, 651, 939
XapI RAATTY 3 cut(s) 166, 484, 774
XcmI CCANNNNNNNNNTGG 2 cut(s) 728, 933
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.