MD01G1052300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011874
Physical Location & Seq
Forward (+)
15713731 .. 15716185
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1052300.v1.1.491

Sequence Viewer

Length: 258 bp
ATGGGAGGAGGTGAGCACGCACACGGAGATGGAGCCCACGGCGGCGATTTCAGGGCTAAGGTGTGGAGTATGAGTGGTGGGCCGTACTGTAGGCCCAAGCACTGGAAGCGCAACACCGCCATCGCCATGGCCGGCATCTTCCTTGTCTGTATTCCTATCGCCATGAAATCTGCCGAGCTCGAGCAACGGCCACATAATCCTGTTCGCCCGATTCCTTCACAACTCTGGTGCAAGAACTTTGGAAAGAAAGACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

86

Amino Acids

9.4

Weight (kDa)

9.6

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 102
AciI CCGC 2 cut(s) 42, 117
AcoI YGGCCR 2 cut(s) 129, 188
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 102
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw21I GWGCWC 2 cut(s) 18, 180
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 4 cut(s) 80, 92, 129, 188
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 80, 93
AsuHPI GGTGA 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 179
BanII GRGCYC 2 cut(s) 37, 180
Bbv12I GWGCWC 2 cut(s) 18, 180
BccI CCATC 2 cut(s) 23, 128
BceAI ACGGC 3 cut(s) 55, 67, 203
BfmI CTRYAG 1 cut(s) 88
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 2 cut(s) 80, 93
BmiI GGNNCC 1 cut(s) 34
BmsI GCATC 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 37, 126
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 107
BseDI CCNNGG 2 cut(s) 37, 126
BseLI CCNNNNNNNGG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 107
BseRI GAGGAG 1 cut(s) 21
BshFI GGCC 4 cut(s) 82, 94, 131, 190
BsiHKAI GWGCWC 2 cut(s) 18, 180
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 102
BsnI GGCC 4 cut(s) 82, 94, 131, 190
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 18, 37, 180
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 2 cut(s) 42, 117
BspANI GGCC 4 cut(s) 82, 94, 131, 190
BspLI GGNNCC 1 cut(s) 34
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 2 cut(s) 37, 126
BssT1I CCWWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 18, 133
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 2 cut(s) 37, 126
BstHHI GCGC 1 cut(s) 111
BstMWI GCNNNNNNNGC 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 88
BstXI CCANNNNNNTGG 1 cut(s) 127
BsuRI GGCC 4 cut(s) 82, 94, 131, 190
BtgI CCRYGG 2 cut(s) 37, 126
BtgZI GCGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 2 cut(s) 18, 133
CfoI GCGC 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 80, 93
Csp6I GTAC 1 cut(s) 85
CviAII CATG 2 cut(s) 127, 163
CviJI RGCY 7 cut(s) 35, 56, 82, 94, 131, 178, 190
CviKI_1 RGCY 7 cut(s) 35, 56, 82, 94, 131, 178, 190
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 57
EaeI YGGCCR 2 cut(s) 129, 188
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 126
Eco24I GRGCYC 2 cut(s) 37, 180
Eco53kI GAGCTC 1 cut(s) 178
Eco88I CYCGRG 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 178
EcoT14I CCWWGG 1 cut(s) 126
EcoT38I GRGCYC 2 cut(s) 37, 180
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 2 cut(s) 130, 166
FaiI YATR 4 cut(s) 71, 128, 164, 195
FatI CATG 2 cut(s) 126, 162
Fnu4HI GCNGC 1 cut(s) 43
FriOI GRGCYC 2 cut(s) 37, 180
Fsp4HI GCNGC 1 cut(s) 43
GlaI GCGC 1 cut(s) 110
GluI GCNGC 1 cut(s) 43
HaeIII GGCC 4 cut(s) 82, 94, 131, 190
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 111
Hin1II CATG 2 cut(s) 130, 166
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HinfI GANTC 1 cut(s) 211
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 225
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4V TGCA 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 106
HpyF3I CTNAG 1 cut(s) 57
Hsp92II CATG 2 cut(s) 130, 166
HspAI GCGC 1 cut(s) 109
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
LmnI GCTCC 1 cut(s) 32
LpnPI CCDG 5 cut(s) 37, 88, 145, 211, 213
LweI GCATC 1 cut(s) 144
MboII GAAGA 1 cut(s) 130
MhlI GDGCHC 3 cut(s) 18, 37, 180
MroNI GCCGGC 1 cut(s) 131
MslI CAYNNNNRTG 2 cut(s) 27, 125
MspI CCGG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 106
NaeI GCCGGC 1 cut(s) 133
NcoI CCATGG 1 cut(s) 126
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 2 cut(s) 130, 166
NlaIV GGNNCC 1 cut(s) 34
NmeAIII GCCGAG 1 cut(s) 199
PaeR7I CTCGAG 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 102
PkrI GCNGC 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 34
PspPI GGNCC 2 cut(s) 80, 93
PspXI VCTCGAGB 1 cut(s) 179
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 2 cut(s) 27, 125
SacI GAGCTC 1 cut(s) 180
SatI GCNGC 1 cut(s) 43
Sau96I GGNCC 2 cut(s) 80, 93
SduI GDGCHC 3 cut(s) 18, 37, 180
SetI ASST 3 cut(s) 13, 63, 180
SfaNI GCATC 1 cut(s) 144
SfcI CTRYAG 1 cut(s) 88
Sfr274I CTCGAG 1 cut(s) 179
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 2 cut(s) 27, 125
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SsiI CCGC 2 cut(s) 42, 117
SstI GAGCTC 1 cut(s) 180
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 89
TaqI TCGA 1 cut(s) 180
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 1 cut(s) 211
TscAI CASTG 1 cut(s) 107
TspDTI ATGAA 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 1 cut(s) 107
Van91I CCANNNNNTGG 1 cut(s) 102
XhoI CTCGAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.