Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007831

Physical Location & Seq

Genomic Coordinates Reverse (-)

15845227 .. 15854875

Gene Structure 9649 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1053700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3669 bp

ATGGATCTTGCGTGTACTTTTCCGCAGCCAAATGTGTTATATGCCATTGAAGGGGCTAGTTATAAGAGGTTGAATTGCTTCAGTTCACCCATTATCTTTGGGAGTAAAGATGTTAGTTGTAACTTTCTGGGAAATTCGAGAATTGTTGTGAAAGCATGTTCGGGTAAGAAGTTGAAGAGAACCGTTTGTGTAAGTGGTTGTAGGATTTCACGTTTGGCCTATAGAGAAAAAAGTGATGATCATTTGTGGAATTCGAATCCGAAAGTGCCTTTATCTTGTAACTTCAGCAGTGTTTTCAAAGGGTCAAGAGCAGTTTGGTGGTCGCGGTGTCAGAATAATGACTCATTAGCGTATGTTAATGGAAATGATCGGAATATAGAATACCTAGAAGGTCATGATGAGAGCACGGGAGTTGAGTCTGTTCATGATGCCGAGTTGACTGATTCCAGAGAAAAGGATGGACATGATGAACAAAAAGAAGAACCAGAGGCACCTACATTGGATGAATTGAAGGAATTGCTGCAAAATGCCATGAAAGAACTGGAAGCTGCACGGCTTAATAGTACCATGTTTGAAGAAAAGGCTCAGAGAATATCAGAAGCTGCGATATCCCTGCAGGATGAAGCAGCAAATGCTTGGAATAATGTCAATTTAACCCTTGATACTGTCCAAGAGATCGTTAATGAAGAGACTATTACCAAAGAAGGTGTTCAGAAAGCAACAATGGCACTTTCATTGGCAGAGGCAAGGCTTCAGGTGGCAGTAGAATCTTTAGGGGTTGCAAAAAGAGAGAGAAGTTCGTCTGAAATTTCACAAGAGAATGATGGGGAACATGATTGCAAAGAAGAAGAAAAAGCACTTTTGGTTACCCAGGAAGATTTTAAGGAATGCCAGGCAAATTTGGCCATCTGTGAGGCAGAGCTAATGCACTTGCAGAGTAAAAAAGAGGAGTTGCAGAAGGAAGTGGACAGGTTGAATGAGGTTGCAGAGAAAGCACAGCTCAGTGCTTTGAAAGCAGAAGAGGATGTAACCAACATAATGCTTCTAGCAGAGCAAGCTGTTGCTTTTGAACTTGAAGCTGCAAAGCGTGTCAACGATGCAGAAATTGCATTACAAAGAGCAGAGAAATCTAGTTCCAACTCAATTGTTGATACCACGGAAAAAGGACAGGTGTTGAATGATGATAATGCTGCTCTTGAGGAAGAGGAGACGGTAGTCCTAGGAAGTTCTGCTGATATCATTGTTGAAAGGGACAGAGATGTGCCAATTGATGGTGATTTGTTAGCTGTCAAGCCTTTGACAGACAGCCTTTCTGACAAAATCAGCCAGAGTTTTGAAGATGCGAATCATTCTGTTGATCTGAGTGATCATGAGAATGGAAAGTTAAGTTTGGATTCTTTAAAAGATGCTGAAGTAGAAGCTGAAAAGTCAAAGAATGTGGTTCAAGCTAAAAAGCAGGAAACTCAGAAGGACTTGAGTAGGGAGAGTTCACCGTTAAATTCTCCCAAGACATTATCGAAGAAATCTTCTCGTTTCTTTTCTGCATCTTTCTTCTCTTCTGCAGATGGAACGCCGACATCAGTTTTCCAGGGTCTGATGGAGTATGCAAGGAAGCAATGGCCAAAGCTTGTTGTTGGGATGTTCCTGTTTGGAGTAGGGCTCACCTTTTATGCTAATCGAGCAGAAAGGGCTACACAACTGTTACAACAACCAGATGTCATGACCACTAGTATTGAAGAAGTTTCCTCAAGTGCTAAGCCTCTAGTTCGAGAATTACAGAAACTTCCTAGAAGATTTAAAAAACTAATTGATATGATTCCTCATCAAGAGGTGAATGAGGAGGAAGCTTCTCTCTTTGACATGTTATGGTTATTGTTAGCAAGTGTGATATTTGTGCCTGTATTTCAAAGGATTCCTGGAGGCAGTCCTGTTCTTGGGTACTTAGCTGCCGGCATCTTGATTGGGCCTTATGGCCTCTCTATTATTCGTCATGTACATGGGACAAAGGCAATTGCTGAATTTGGAGTTGTTTTCTTGTTGTTCAACATTGGCCTCGAGCTTTCTGTTGAAAGGTTGAGTTCAATGAAGAAATATGTATTTGGATTAGGCTCTGCTCAGGTTTTGGTGACAGCTGTTGTGGTTGGTGTGGTTGCTCATTATGTTTGTGGGCTGCCTGGTCCTGCTGCAATAGTAATTGGCAATGGTCTGGCTTTATCATCCACTGCAGTTGTTTTGCAGGTCTTGCAAGAGCGGGGTGAGAGCACTTCACGACATGGCCGTGCTACGTTTTCTGTCCTACTTTTCCAGGATTTGGCTGTTGTTGTTTTGCTAATTCTTATACCTCTGATTTCACCAAATTCATCCAAAGGAGGGATTGGTTTTCAAGCCATTGCTGAAGCTCTTGGACTTGCTGCTGTAAAGGCAGCAGTTGCCATCACAGCCATAATTGCTGGTGGGCGCTTGCTGCTTCGACCAATTTATAGGCAAATTGCAGAAAATCAAAATGCAGAAATATTTTCTGCCAATACATTGCTTGTTATTCTAGGAACAAGTCTTCTCACTGCCAGGGCTGGACTATCCATGGCACTGGGAGCATTTTTGGCTGGTTTGCTGCTTGCAGAAACTGAATTTTCTTTACAGGTTGAGTCAGATATTGCTCCATACCGTGGTCTTCTATTGGGCCTCTTCTTTATGACGGTTGGAATGTCTATTGATCCAAAACTTCTTCTTTCAAACTTCCCGGTTATAGCTGGATCATTAGGACTCCTAATTGGTGGCAAATCCTTATTGGTTGCTTTGATTGGCAAACTGTTTGGCGTTTCAATAATATCTGCAATAAGAGTTGGTCTTCTTCTTGCTCCTGGTGGAGAGTTTGCTTTTGTTGCTTTTGGTGAAGCTGTTAATCAGGGAATAATGACTCCTCAATTATCATCATTGCTCTTCCTTCTTGTGGGAATTTCAATGGCCATCACACCATGGCTAGCTGCTGGAGGGCAGTTAATTGCATCTCGTTTTGAGGTGCATGACGTTCGAAGTTTACTACCTGTTGAGAGTGAGACAGATGATTTACAAGGTCATATAATCATCTGTGGATTTGGGCGAGTTGGCCAGATAATTGCACAGCTTCTTTCTGAGAGATTAATTCCGTTTGTAGCTCTTGATGTGAGGAGTGATAGAGTGGCAGTTGGACGCTCGCTGGACCTTCCAGTCTACTTTGGAGATGCTGGTAGCCGAGAGGTGCTCCACAAAATTGGTGCTGAAAGAGCATGTGCTGCAGCGATCACTTTAGATTCTCCTGGTGCAAACTATAGAACTGTTTGGGCTCTGAGCAAGTATTTCCCGAACGTCAAAACTTTTGTTCGTGCTCATGACGTCGACCATGGTCTTAATTTGGAGAAGGCTGGGGCTACAGCGGTTGTCCCAGAGACCTTGGAACCGAGTCTGCAGTTGGCAGCTGCTGTTCTCGCTCAGGCTAAACTACCCATGTCAGAGATCACATCAACAATCAACGAGTATAGGTCCCGTCACCTTGCGGAGCTAACTGAGTTATGTGAAACTAGCGGAAGTTCTCTTGGCTACGGATTTTCTCGAATGATGAGTAAACCCAAAACTCCGCCTCCAGATTCCACAGATGAGAACCAGTTCACTGAAGGAACACTGGCGATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008324 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010109 GO:0015075 GO:0015077 GO:0015078 GO:0015079 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015386 GO:0015491 GO:0015672 GO:0016020 GO:0016043 GO:0019222 GO:0022804 GO:0022821 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031323 GO:0031967 GO:0031969 GO:0031975 GO:0034220 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071804 GO:0071805 GO:0071840 GO:0098655 GO:0098660 GO:0098662 GO:0099516 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MutS_I MutS_II MutS_III MutS_V Na_H_Exchanger TrkA_N

Protein Analysis

1223

Amino Acids

132.39

Weight (kDa)

5.09

Isoelectric Point (pI)

41.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	628 - 994	2.6e-55	Sodium/hydrogen exchanger, transmembrane
TrkA_N	PF02254	1027 - 1140	6.8e-21	TrkA-N domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007831)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00630 AT4G00630
fragaria_vesca	FvH4_7g13660 FvH4_7g13660 FvH4_7g13660 FvH4_7g13660 FvH4_7g13660
malus_domestica	MD01G1053700.v1.1 MD07G1141400.v1.1
prunus_persica	Prupe.2G162700_v2.0.a1 Prupe.2G162700_v2.0.a1
pyrus_communis	pycom01g07930 pycom07g14030
rosa_chinensis	RchiOBHm_Chr1g0353741
rosa_laevigata	RLG00000028262
rosa_multiflora	Rmu_sc0005947.1_g000021
rosa_roxburghii	Rroxscaffold_4G00301700
rosa_rugosa	Rorug01G0237300 Rorug01G0237300
rosa_samantha	Rh1AG249500 Rh1BG219600 Rh1CG232200 Rh1DG246600
rosa_wichuraiana	Rw1G021770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	63
AasI	GACNNNNNNGTC	1 cut(s)	3206
AatII	GACGTC	1 cut(s)	3375
Acc36I	ACCTGC	1 cut(s)	2227
AccB1I	GGYRCC	1 cut(s)	490
AccB7I	CCANNNNNTGG	3 cut(s)	1271, 2311, 2666
AccBSI	CCGCTC	1 cut(s)	2251
AccI	GTMKAC	2 cut(s)	3210, 3375
AccII	CGCG	1 cut(s)	325
AciI	CCGC	7 cut(s)	23, 325, 2251, 3413, 3533, 3561, 3614
AclWI	GGATC	3 cut(s)	12, 2708, 2761
AcoI	YGGCCR	5 cut(s)	903, 1619, 2275, 2964, 3106
AcsI	RAATTY	9 cut(s)	133, 250, 807, 898, 1498, 2018, 2356, 2627, 2955
AcuI	CTGAAG	6 cut(s)	64, 268, 737, 1431, 2415, 3669
AcyI	GRCGYC	1 cut(s)	3372
AfaI	GTAC	4 cut(s)	16, 565, 1940, 1995
AfiI	CCNNNNNNNGG	7 cut(s)	51, 1271, 1934, 2311, 2370, 2666, 2950
AflIII	ACRYGT	1 cut(s)	1860
AhlI	ACTAGT	1 cut(s)	1727
AjnI	CCWGG	9 cut(s)	870, 891, 1587, 1915, 2173, 2304, 2564, 2860, 3295
Alw21I	GWGCWC	4 cut(s)	407, 2264, 3243, 3367
Alw26I	GTCTC	4 cut(s)	683, 1202, 3050, 3419
AlwI	GGATC	3 cut(s)	12, 2708, 2761
AlwNI	CAGNNNCTG	4 cut(s)	602, 1594, 2624, 3458
Ama87I	CYCGRG	1 cut(s)	2054
ApoI	RAATTY	9 cut(s)	133, 250, 807, 898, 1498, 2018, 2356, 2627, 2955
AseI	ATTAAT	1 cut(s)	3140
Asp700I	GAANNNNTTC	3 cut(s)	77, 708, 3641
AspA2I	CCTAGG	1 cut(s)	1219
AspLEI	GCGC	1 cut(s)	2460
AspS9I	GGNCC	5 cut(s)	1964, 2177, 2680, 3199, 3519
AsuC2I	CCSGG	1 cut(s)	2741
AsuII	TTCGAA	2 cut(s)	254, 3031
AsuNHI	GCTAGC	1 cut(s)	2980
AvaI	CYCGRG	1 cut(s)	2054
AvaII	GGWCC	3 cut(s)	2177, 3199, 3519
AvrII	CCTAGG	1 cut(s)	1219
BalI	TGGCCA	4 cut(s)	905, 1621, 2966, 3108
BanI	GGYRCC	1 cut(s)	490
BanII	GRGCYC	2 cut(s)	1662, 3325
BbsI	GAAGAC	3 cut(s)	2546, 2663, 2840
Bbv12I	GWGCWC	4 cut(s)	407, 2264, 3243, 3367
BccI	CCATC	8 cut(s)	452, 818, 914, 1265, 1559, 1591, 2441, 2975
BceAI	ACGGC	2 cut(s)	569, 2262
BcgI	CGANNNNNNTGC	4 cut(s)	595, 629, 3011, 3045
BciT130I	CCWGG	9 cut(s)	872, 893, 1589, 1917, 2175, 2306, 2566, 2862, 3297
BclI	TGATCA	2 cut(s)	238, 1366
BcnI	CCSGG	1 cut(s)	2741
BcoDI	GTCTC	4 cut(s)	683, 1202, 3050, 3419
BcuI	ACTAGT	1 cut(s)	1727
BfmI	CTRYAG	8 cut(s)	220, 614, 1560, 2223, 3273, 3307, 3408, 3443
BfoI	RGCGCY	1 cut(s)	2461
BfuAI	ACCTGC	1 cut(s)	2227
BlnI	CCTAGG	1 cut(s)	1219
BlpI	GCTNAGC	1 cut(s)	1755
Bme18I	GGWCC	3 cut(s)	2177, 3199, 3519
BmeT110I	CYCGRG	1 cut(s)	2054
BmgT120I	GGNCC	5 cut(s)	1964, 2177, 2680, 3199, 3519
BmiI	GGNNCC	3 cut(s)	492, 3435, 3521
BmrI	ACTGGG	1 cut(s)	2597
BmsI	GCATC	8 cut(s)	418, 1087, 1330, 1396, 1553, 1962, 3014, 3211
BmtI	GCTAGC	1 cut(s)	2984
BmuI	ACTGGG	1 cut(s)	2597
BoxI	GACNNNNGTC	2 cut(s)	1214, 3381
BpiI	GAAGAC	3 cut(s)	2546, 2663, 2840
BplI	GAGNNNNNCTC	4 cut(s)	1644, 1676, 3225, 3257
BpmI	CTGGAG	3 cut(s)	1938, 3009, 3603
Bpu10I	CCTNAGC	2 cut(s)	2115, 3468
Bpu1102I	GCTNAGC	1 cut(s)	1755
Bpu14I	TTCGAA	2 cut(s)	254, 3031
BpuEI	CTTGAG	3 cut(s)	1217, 1495, 1732
BpuMI	CCSGG	1 cut(s)	2741
BsaBI	GATNNNNATC	1 cut(s)	1344
BsaHI	GRCGYC	1 cut(s)	3372
BsaI	GGTCTC	1 cut(s)	3419
BsaXI	ACNNNNNCTCC	2 cut(s)	3601, 3631
Bsc4I	CCNNNNNNNGG	7 cut(s)	51, 1271, 1934, 2311, 2370, 2666, 2950
Bse118I	RCCGGY	1 cut(s)	1949
Bse1I	ACTGG	5 cut(s)	546, 2592, 3206, 3640, 3663
Bse3DI	GCAATG	5 cut(s)	1622, 2206, 2388, 2528, 2933
Bse8I	GATNNNNATC	1 cut(s)	1344
BseBI	CCWGG	9 cut(s)	872, 893, 1589, 1917, 2175, 2306, 2566, 2862, 3297
BseGI	GGATG	7 cut(s)	463, 508, 625, 1030, 1644, 2216, 2360
BseJI	GATNNNNATC	1 cut(s)	1344
BseLI	CCNNNNNNNGG	7 cut(s)	51, 1271, 1934, 2311, 2370, 2666, 2950
BseMI	GCAATG	5 cut(s)	1622, 2206, 2388, 2528, 2933
BseMII	CTCAG	9 cut(s)	599, 1015, 1352, 1478, 2129, 3123, 3317, 3482, 3534
BseNI	ACTGG	5 cut(s)	546, 2592, 3206, 3640, 3663
BseRI	GAGGAG	5 cut(s)	962, 1220, 1853, 2910, 3181
BseYI	CCCAGC	1 cut(s)	3401
BsgI	GTGCAG	1 cut(s)	534
Bsh1236I	CGCG	1 cut(s)	325
BshNI	GGYRCC	1 cut(s)	490
BsiHKAI	GWGCWC	4 cut(s)	407, 2264, 3243, 3367
BsiHKCI	CYCGRG	1 cut(s)	2054
BsiSI	CCGG	2 cut(s)	1950, 2741
BslFI	GGGAC	4 cut(s)	1265, 2014, 3404, 3505
BslI	CCNNNNNNNGG	7 cut(s)	51, 1271, 1934, 2311, 2370, 2666, 2950
BsmAI	GTCTC	4 cut(s)	683, 1202, 3050, 3419
BsmBI	CGTCTC	1 cut(s)	1202
BsmFI	GGGAC	4 cut(s)	1265, 2014, 3404, 3505
BsmI	GAATGC	1 cut(s)	893
Bso31I	GGTCTC	1 cut(s)	3419
BsoBI	CYCGRG	1 cut(s)	2054
Bsp119I	TTCGAA	2 cut(s)	254, 3031
Bsp1286I	GDGCHC	6 cut(s)	407, 1662, 2264, 3243, 3325, 3367
Bsp1407I	TGTACA	1 cut(s)	1993
Bsp1720I	GCTNAGC	1 cut(s)	1755
Bsp19I	CCATGG	3 cut(s)	2580, 2975, 3379
BspACI	CCGC	7 cut(s)	23, 325, 2251, 3413, 3533, 3561, 3614
BspCNI	CTCAG	9 cut(s)	598, 1014, 1353, 1477, 2128, 3124, 3318, 3481, 3535
BspFNI	CGCG	1 cut(s)	325
BspHI	TCATGA	5 cut(s)	394, 424, 1369, 1719, 3367
BspLI	GGNNCC	3 cut(s)	492, 3435, 3521
BspMAI	CTGCAG	5 cut(s)	618, 1564, 2227, 3277, 3447
BspMI	ACCTGC	1 cut(s)	2227
BspOI	GCTAGC	1 cut(s)	2984
BspPI	GGATC	3 cut(s)	12, 2708, 2761
BspQI	GCTCTTC	1 cut(s)	2945
BspT104I	TTCGAA	2 cut(s)	254, 3031
BspT107I	GGYRCC	1 cut(s)	490
BspTNI	GGTCTC	1 cut(s)	3419
BsrBI	CCGCTC	1 cut(s)	2251
BsrDI	GCAATG	5 cut(s)	1622, 2206, 2388, 2528, 2933
BsrFI	RCCGGY	1 cut(s)	1949
BsrGI	TGTACA	1 cut(s)	1993
BsrI	ACTGG	5 cut(s)	546, 2592, 3206, 3640, 3663
BssAI	RCCGGY	1 cut(s)	1949
BssNI	GRCGYC	1 cut(s)	3372
BssT1I	CCWWGG	5 cut(s)	1219, 2580, 2975, 3379, 3429
Bst2UI	CCWGG	9 cut(s)	872, 893, 1589, 1917, 2175, 2306, 2566, 2862, 3297
Bst4CI	ACNGT	9 cut(s)	184, 667, 1213, 1494, 1701, 2666, 2698, 2811, 3316
Bst6I	CTCTTC	7 cut(s)	170, 681, 1014, 1197, 1561, 2690, 2945
BstACI	GRCGYC	1 cut(s)	3372
BstAPI	GCANNNNNTGC	5 cut(s)	632, 1106, 2242, 2429, 3272
BstAUI	TGTACA	1 cut(s)	1993
BstBI	TTCGAA	2 cut(s)	254, 3031
BstC8I	GCNNGC	6 cut(s)	1056, 1951, 2462, 2616, 2982, 3194
BstDSI	CCRYGG	5 cut(s)	1155, 2580, 2665, 2975, 3379
BstEII	GGTNACC	1 cut(s)	865
BstF5I	GGATG	7 cut(s)	463, 508, 625, 1030, 1644, 2216, 2360
BstFNI	CGCG	1 cut(s)	325
BstH2I	RGCGCY	1 cut(s)	2461
BstHHI	GCGC	1 cut(s)	2460
BstMAI	GTCTC	4 cut(s)	683, 1202, 3050, 3419
BstNI	CCWGG	9 cut(s)	872, 893, 1589, 1917, 2175, 2306, 2566, 2862, 3297
BstNSI	RCATGY	3 cut(s)	159, 1864, 3270
BstPAI	GACNNNNGTC	2 cut(s)	1214, 3381
BstPI	GGTNACC	1 cut(s)	865
BstSFI	CTRYAG	8 cut(s)	220, 614, 1560, 2223, 3273, 3307, 3408, 3443
BstUI	CGCG	1 cut(s)	325
BstV2I	GAAGAC	3 cut(s)	2546, 2663, 2840
BstX2I	RGATCY	1 cut(s)	4
BstXI	CCANNNNNNTGG	2 cut(s)	2587, 3251
BstYI	RGATCY	1 cut(s)	4
BtgI	CCRYGG	5 cut(s)	1155, 2580, 2665, 2975, 3379
BtsCI	GGATG	7 cut(s)	463, 508, 625, 1030, 1644, 2216, 2360
BtsI	GCAGTG	3 cut(s)	295, 2220, 2559
BtsIMutI	CAGTG	7 cut(s)	295, 1009, 2220, 2559, 2585, 3645, 3656
BveI	ACCTGC	1 cut(s)	2227
Cac8I	GCNNGC	6 cut(s)	1056, 1951, 2462, 2616, 2982, 3194
CaiI	CAGNNNCTG	4 cut(s)	602, 1594, 2624, 3458
CciI	TCATGA	5 cut(s)	394, 424, 1369, 1719, 3367
CfoI	GCGC	1 cut(s)	2460
Cfr10I	RCCGGY	1 cut(s)	1949
Cfr13I	GGNCC	5 cut(s)	1964, 2177, 2680, 3199, 3519
CseI	GACGC	1 cut(s)	3198
Csp6I	GTAC	4 cut(s)	15, 564, 1939, 1994
CspCI	CAANNNNNGTGG	6 cut(s)	1419, 1454, 1714, 1749, 2209, 2244
CviQI	GTAC	4 cut(s)	15, 564, 1939, 1994
DraI	TTTAAA	2 cut(s)	1401, 1798
DrdI	GACNNNNNNGTC	1 cut(s)	3206
DseDI	GACNNNNNNGTC	1 cut(s)	3206
EaeI	YGGCCR	5 cut(s)	903, 1619, 2275, 2964, 3106
Eam1104I	CTCTTC	7 cut(s)	170, 681, 1014, 1197, 1561, 2690, 2945
EarI	CTCTTC	7 cut(s)	170, 681, 1014, 1197, 1561, 2690, 2945
EciI	GGCGGA	1 cut(s)	3603
Eco130I	CCWWGG	5 cut(s)	1219, 2580, 2975, 3379, 3429
Eco24I	GRGCYC	2 cut(s)	1662, 3325
Eco31I	GGTCTC	1 cut(s)	3419
Eco32I	GATATC	2 cut(s)	609, 1237
Eco47I	GGWCC	3 cut(s)	2177, 3199, 3519
Eco57I	CTGAAG	6 cut(s)	64, 268, 737, 1431, 2415, 3669
Eco88I	CYCGRG	1 cut(s)	2054
Eco91I	GGTNACC	1 cut(s)	865
EcoO109I	RGGNCCY	1 cut(s)	3519
EcoO65I	GGTNACC	1 cut(s)	865
EcoRI	GAATTC	1 cut(s)	250
EcoRII	CCWGG	9 cut(s)	870, 891, 1587, 1915, 2173, 2304, 2564, 2860, 3295
EcoRV	GATATC	2 cut(s)	609, 1237
EcoT14I	CCWWGG	5 cut(s)	1219, 2580, 2975, 3379, 3429
EcoT38I	GRGCYC	2 cut(s)	1662, 3325
ErhI	CCWWGG	5 cut(s)	1219, 2580, 2975, 3379, 3429
Esp3I	CGTCTC	1 cut(s)	1202
FaqI	GGGAC	4 cut(s)	1265, 2014, 3404, 3505
FauI	CCCGC	1 cut(s)	2244
FbaI	TGATCA	2 cut(s)	238, 1366
FblI	GTMKAC	2 cut(s)	3210, 3375
FokI	GGATG	7 cut(s)	470, 515, 632, 1037, 1651, 2203, 2347
FriOI	GRGCYC	2 cut(s)	1662, 3325
GlaI	GCGC	1 cut(s)	2459
GsaI	CCCAGC	1 cut(s)	3405
GsuI	CTGGAG	3 cut(s)	1938, 3009, 3603
HaeII	RGCGCY	1 cut(s)	2461
HapII	CCGG	2 cut(s)	1950, 2741
HgaI	GACGC	1 cut(s)	3198
HhaI	GCGC	1 cut(s)	2460
Hin1I	GRCGYC	1 cut(s)	3372
Hin6I	GCGC	1 cut(s)	2458
HinP1I	GCGC	1 cut(s)	2458
HincII	GTYRAC	3 cut(s)	438, 1093, 3376
HindII	GTYRAC	3 cut(s)	438, 1093, 3376
HindIII	AAGCTT	2 cut(s)	1625, 1845
HpaII	CCGG	2 cut(s)	1950, 2741
Hpy99I	CGWCG	1 cut(s)	3377
HpyCH4III	ACNGT	9 cut(s)	184, 667, 1213, 1494, 1701, 2666, 2698, 2811, 3316
HpyCH4IV	ACGT	5 cut(s)	211, 2285, 3027, 3345, 3372
HpySE526I	ACGT	5 cut(s)	211, 2285, 3027, 3345, 3372
Hsp92I	GRCGYC	1 cut(s)	3372
HspAI	GCGC	1 cut(s)	2458
KroI	GCCGGC	1 cut(s)	1949
KroNI	GCCGGC	1 cut(s)	1951
Ksp22I	TGATCA	2 cut(s)	238, 1366
LguI	GCTCTTC	1 cut(s)	2945
LmnI	GCTCC	5 cut(s)	2591, 2662, 2863, 3246, 3535
LweI	GCATC	8 cut(s)	418, 1087, 1330, 1396, 1553, 1962, 3014, 3211
MaeII	ACGT	5 cut(s)	211, 2285, 3027, 3345, 3372
MaeIII	GTNAC	7 cut(s)	119, 278, 865, 1027, 1701, 2125, 3524
MbiI	CCGCTC	1 cut(s)	2251
MfeI	CAATTG	3 cut(s)	1143, 1266, 2010
MflI	RGATCY	1 cut(s)	4
MhlI	GDGCHC	6 cut(s)	407, 1662, 2264, 3243, 3325, 3367
MlsI	TGGCCA	4 cut(s)	905, 1621, 2966, 3108
MluNI	TGGCCA	4 cut(s)	905, 1621, 2966, 3108
MlyI	GAGTC	6 cut(s)	335, 425, 2654, 2757, 2911, 3448
MmeI	TCCRAC	3 cut(s)	1161, 2680, 3166
Mox20I	TGGCCA	4 cut(s)	905, 1621, 2966, 3108
MroNI	GCCGGC	1 cut(s)	1949
MroXI	GAANNNNTTC	3 cut(s)	77, 708, 3641
MscI	TGGCCA	4 cut(s)	905, 1621, 2966, 3108
MslI	CAYNNNNRTG	3 cut(s)	1374, 1995, 2277
Msp20I	TGGCCA	4 cut(s)	905, 1621, 2966, 3108
MspA1I	CMGCKG	3 cut(s)	2132, 3413, 3455
MspI	CCGG	2 cut(s)	1950, 2741
MunI	CAATTG	3 cut(s)	1143, 1266, 2010
Mva1269I	GAATGC	1 cut(s)	893
MvaI	CCWGG	9 cut(s)	872, 893, 1589, 1917, 2175, 2306, 2566, 2862, 3297
MvnI	CGCG	1 cut(s)	325
NaeI	GCCGGC	1 cut(s)	1951
NciI	CCSGG	1 cut(s)	2741
NcoI	CCATGG	3 cut(s)	2580, 2975, 3379
NgoMIV	GCCGGC	1 cut(s)	1949
NheI	GCTAGC	1 cut(s)	2980
NlaIV	GGNNCC	3 cut(s)	492, 3435, 3521
NmeAIII	GCCGAG	2 cut(s)	457, 3257
NmuCI	GTSAC	2 cut(s)	2125, 3524
NspI	RCATGY	3 cut(s)	159, 1864, 3270
NspV	TTCGAA	2 cut(s)	254, 3031
PaeR7I	CTCGAG	1 cut(s)	2054
PagI	TCATGA	5 cut(s)	394, 424, 1369, 1719, 3367
PciI	ACATGT	1 cut(s)	1860
PciSI	GCTCTTC	1 cut(s)	2945
PcsI	WCGNNNNNNNCGW	1 cut(s)	2275
PctI	GAATGC	1 cut(s)	893
PdiI	GCCGGC	1 cut(s)	1951
PdmI	GAANNNNTTC	3 cut(s)	77, 708, 3641
PfeI	GAWTC	9 cut(s)	256, 443, 767, 1345, 1394, 1816, 1912, 3290, 3623
PflMI	CCANNNNNTGG	3 cut(s)	1271, 2311, 2666
PfoI	TCCNGGA	2 cut(s)	1915, 2304
PleI	GAGTC	6 cut(s)	335, 424, 2653, 2757, 2911, 3447
PpsI	GAGTC	6 cut(s)	335, 424, 2653, 2757, 2911, 3447
PpuMI	RGGWCCY	1 cut(s)	3519
PscI	ACATGT	1 cut(s)	1860
PshAI	GACNNNNGTC	2 cut(s)	1214, 3381
PshBI	ATTAAT	1 cut(s)	3140
PsiI	TTATAA	1 cut(s)	63
Psp5II	RGGWCCY	1 cut(s)	3519
Psp6I	CCWGG	9 cut(s)	870, 891, 1587, 1915, 2173, 2304, 2564, 2860, 3295
PspEI	GGTNACC	1 cut(s)	865
PspFI	CCCAGC	1 cut(s)	3401
PspGI	CCWGG	9 cut(s)	870, 891, 1587, 1915, 2173, 2304, 2564, 2860, 3295
PspN4I	GGNNCC	3 cut(s)	492, 3435, 3521
PspPI	GGNCC	5 cut(s)	1964, 2177, 2680, 3199, 3519
PspPPI	RGGWCCY	1 cut(s)	3519
PspXI	VCTCGAGB	1 cut(s)	2054
PsrI	GAACNNNNNNTAC	2 cut(s)	1471, 1503
PstI	CTGCAG	5 cut(s)	618, 1564, 2227, 3277, 3447
PstNI	CAGNNNCTG	4 cut(s)	602, 1594, 2624, 3458
PsuI	RGATCY	1 cut(s)	4
PvuII	CAGCTG	2 cut(s)	2132, 3455
RsaI	GTAC	4 cut(s)	16, 565, 1940, 1995
RsaNI	GTAC	4 cut(s)	15, 564, 1939, 1994
RseI	CAYNNNNRTG	3 cut(s)	1374, 1995, 2277
SalI	GTCGAC	1 cut(s)	3374
SapI	GCTCTTC	1 cut(s)	2945
Sau96I	GGNCC	5 cut(s)	1964, 2177, 2680, 3199, 3519
SbfI	CCTGCAGG	1 cut(s)	618
SchI	GAGTC	6 cut(s)	335, 425, 2654, 2757, 2911, 3448
SdaI	CCTGCAGG	1 cut(s)	618
SduI	GDGCHC	6 cut(s)	407, 1662, 2264, 3243, 3325, 3367
SfaNI	GCATC	8 cut(s)	418, 1087, 1330, 1396, 1553, 1962, 3014, 3211
SfcI	CTRYAG	8 cut(s)	220, 614, 1560, 2223, 3273, 3307, 3408, 3443
Sfr274I	CTCGAG	1 cut(s)	2054
SfuI	TTCGAA	2 cut(s)	254, 3031
SinI	GGWCC	3 cut(s)	2177, 3199, 3519
SlaI	CTCGAG	1 cut(s)	2054
SmiMI	CAYNNNNRTG	3 cut(s)	1374, 1995, 2277
SmlI	CTYRAG	4 cut(s)	1196, 1474, 1747, 2054
SmoI	CTYRAG	4 cut(s)	1196, 1474, 1747, 2054
SpeI	ACTAGT	1 cut(s)	1727
Sse8387I	CCTGCAGG	1 cut(s)	618
SsiI	CCGC	7 cut(s)	23, 325, 2251, 3413, 3533, 3561, 3614
SspI	AATATT	1 cut(s)	2514
StyI	CCWWGG	5 cut(s)	1219, 2580, 2975, 3379, 3429
TaaI	ACNGT	9 cut(s)	184, 667, 1213, 1494, 1701, 2666, 2698, 2811, 3316
TaiI	ACGT	5 cut(s)	214, 2288, 3030, 3348, 3375
TatI	WGTACW	2 cut(s)	14, 1993
TfiI	GAWTC	9 cut(s)	256, 443, 767, 1345, 1394, 1816, 1912, 3290, 3623
TscAI	CASTG	7 cut(s)	295, 1009, 2227, 2566, 2592, 3652, 3663
TseFI	GTSAC	2 cut(s)	2125, 3524
Tsp45I	GTSAC	2 cut(s)	2125, 3524
TspDTI	ATGAA	9 cut(s)	413, 483, 519, 548, 636, 699, 723, 2099, 2349
TspGWI	ACGGA	3 cut(s)	1172, 3135, 3594
TspRI	CASTG	7 cut(s)	295, 1009, 2227, 2566, 2592, 3652, 3663
Van91I	CCANNNNNTGG	3 cut(s)	1271, 2311, 2666
VpaK11BI	GGWCC	3 cut(s)	2177, 3199, 3519
VspI	ATTAAT	1 cut(s)	3140
XapI	RAATTY	9 cut(s)	133, 250, 807, 898, 1498, 2018, 2356, 2627, 2955
XceI	RCATGY	3 cut(s)	159, 1864, 3270
XcmI	CCANNNNNNNNNTGG	1 cut(s)	538
XhoI	CTCGAG	1 cut(s)	2054
XmaJI	CCTAGG	1 cut(s)	1219
XmiI	GTMKAC	2 cut(s)	3210, 3375
XmnI	GAANNNNTTC	3 cut(s)	77, 708, 3641
ZraI	GACGTC	1 cut(s)	3373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.