Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007831

Physical Location & Seq

Genomic Coordinates Forward (+)

20509705 .. 20521490

Gene Structure 11786 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1141400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3678 bp

ATGGATTTTCTGTGTAGTTTTCAGCAGCCAAATGTGTTATATGGCAGTGAAGGGGCTAGTTATAAGAGGTTGAATTGCTTTAGTAGTTCACCGATTATCTTTGGGAGTAAAGATGTTAGTTGTAACTTTCTGGGTAATTCGAGAATTGTTGTGAAAGCATGGTCAGGTAAGAAGTTGAAGAGAACTGTCTGTGTAAGTGGTTGTAGGATTTCACGTTTGACCTATAGAGAAAAAGCTGATGATCGTTTGTGGAATTCGAATCCGAAAGTGCCTTTATCTTGTAACTTTGGTAGTGTTTTCAAAGGGTCAAGAACAGTTTGGTGGTCGCGGTGTCAGAGTAATGACTCATTAGCGTATGTTAATGGAAATGGTCAGAATGTGGAATACCTAGAAGGTCATGATGAGAGCTCAGGAGTTGGGTCTGTTCATGATGCCGAGTTGAGTGATTCCAGAGAAGAAGATGGATATGAAGAACAAAAAGAAGAACCAGAGGCACCTACATTGGATGAATTGAACGAATTGCTGCAAAATGCCATGAAAGAGCTTGAAGCTGCACGACTTAATAGTACCATGTTTGAAGAAAAGGCTCAGAGAATATCAGAAGCCGCGATATGCCTACGGGATGAAGCAGCAAATGCTTGGAATAATGTTAACTCAACCCTTGATACTGTCCAAGAGATCGTTCATGAAGAGACTATTGCCAAAGAAGGCGTTCAGAAAGCAAAAATGGCACTTTCATTAGCAGAGGCAAGGCTTCGGGTGGCAGTGGAATCTTTACAGGTTGCAAAAAGAGAGACCAATTCTTCTGAAATTTCACAAGAGAATGATGGGGAACATGATTGCAAAGAAGAAGAAAAAGCACTTTTGGTTGCCCAGGAAGATATTAAGGAATGCCAGGCGAATTTGGCCAACTGTGAGGCAGAGCTAATGCACTTGCATAGTAAAAAAGAGGAGTTGCAGAAGGAAATGGACAGGTTGAATGAGGTTGCAGAGAAAGCACAGCTCAGTGCTTTGAAAGCAGAAGAGGATGTAACCAACATAATGCTTTTAGCAGAGCAAGCTGTTGCTTTTGAACTTGAAGCTGCAAAGCATGTCAACGATGCAGAAATTGCATTACAAAGAGCACAGAAATCTATCTCCAACTCAATGGTTGACACCACAGAAAACAACCAAGGACAGGTGTTGAGTGATGATAATCCTGCTCTTGAGGAAGGGGAGACGGTAGTCCTAGGAAGTTCTGCTGATATCATTGTTGAAAGGGACAGAGATGTGGTAATTGATGGTGATTTGTTGGCTGTCAAGCCTTTGGCAGACAGCCCTTCTGACAAAATCAGCCAGAGTTTTGAAGACGCAAGTCAATTTGTTGATTTGAGTGATCATGAGAATGGAAAGTTAAGTTTGGATTCTCTAAAAGACGCTGACGTAGAAGCTGAAAAGTCAAAGAATGTGGTTCAAGCTAAAAAGCAGGAAACTCAGAAGGACTTGAGTAGGGAGAGTTCACCGTTAAATTCTCCAAAGACATTATTGAAGAAATCTTCTCGTTTCTTTTCTGCATCTTTCTTCTCTTCTGCTGATGGGACTCCGACATCAGTTTTCCAAGGTCTGATGGAGTATGCAAGGAAGCAATGGCCAAAGCTGGTTGTCGGGATGTTCCTATTTGGAGTAGGGCTCACCTTTTATGCTAATCGAGTGGAAAGGGCCACACAACTGTTACAACAGCCAGACGTCATCACCACTAGTATTGAAGAAGTTTCCTCAAGTGCGAAGCCTCTAGTTCGAGAATTACAGAAACTTCCTAGAAGAATTAAAAAACTAATTGATATGATTCCTCATCAAGAGGTGAATGAGGAGGAAGCTTCTCTTTTTGACATGTTATGGTTATTGTTAGCAAGTGTGATATTTGTGCCTGTATTTCAAAGAATTCCTGGAGGCAGTCCTGTTCTTGGGTATTTAGCTGCTGGCATCTTGATTGGGCCTTATGGCCTCTCTATTATTCGTCATGTACATGGGACAAAGGCAATTGCTGAATTTGGGGTTGTTTTCTTGCTGTTCAACATTGGCCTCGAGCTTTCTGTTGAAAGGTTGAGTTCAATGAAGAAATATGTATTTGGATTAGGCTCTGCTCAGGTTTTGGTGACAGCTGTTGTGGTTGGTGTGGTTGCTCATTATGTTTGTGGGCTGCCTGGACCTGCTGCAATAGTAATTGGCAATGGTCTGGCTTTATCATCCACTGCAGTTGTTCTGCAGGTCTTGCAAGAGCGGGGTGAGAGCACTTCACGTCATGGCCGTGCTACGTTTTCTGTCCTACTTTTCCAGGATTTGGCTGTTGTGGTTTTGCTAATTCTTATACCTCTGATTTCACCAAATTCATCCAAAGGAGGGATTGGTTTTCAAGCCATTGCTGAAGCTCTTGGACTTGCTGCTGTAAAGGCAGCAGTTGCCATCACGGCCATAATTGCTGGTGGACGCTTGCTGCTTCGACCAATTTATAGGCAAATTGCAGAAAATCAAAATGCAGAAATATTTTCTGCCAATACTTTGCTTGTTATTCTAGGAACAAGTCTTCTCACTGCCAGGGCTGGACTATCTATGGCATTGGGAGCATTTTTGGCTGGTTTGCTTCTTGCAGAAACTGAATTTTCTTTACAGGTTGAGTCCGATATTGCTCCATATCGTGGTCTTCTACTGGGCCTCTTCTTTATGACGGTTGGAATGTCTATTGATCCGAAACTTCTTCTTTCAAACTTCCCGGTTATAGCTGGATCATTAGGACTTCTGATTGGTGGGAAATCCTTATTGGTTGCTTTGATTGGCAAACTATTTGGCGTTTCAATAATATCTGCAATAAGAGTTGGTCTTCTTCTTGCTCCTGGTGGAGAGTTTGCATTTGTTGCATTTGGTGAAGCTGTTAATCAGGGAATAATGACTCCTCGGTTATCATCTTTGCTTTTCCTTCTTGTTGGAATTTCAATGGCCATCACACCATGGCTAGCTGCTGGAGGGCAGTTAATTGCATCTCGTTTTGAGGTGCATGACGTTCGAAGTTTACTACCAGTCGAGAGTGAGACGGATGATTTACAAGGTCATATAATCATCTGTGGATTTGGGCGAGTTGGCCAGATAATTGCACAGCTTCTTTCTGAGAGTCTAATTCCATTTGTAGCTCTTGATGTGAGGAGTGATAGAGTGGCAGTCGGACGCTCGCTGGACCTTCCAGTCTACTTTGGAGATGCTGGTAGCCGAGAGGTGCTCCACAAAGTTGGTGCTGAAAGAGCATGTGCTGCAGCAATCACTTTAGATTCTCCTGGTGCAAACTATAGAACTGTTTGGGCTCTGAGCAAGTATTTCCCTAACGTGAAAACTTTTGTCCGTGCTCATGATGTTGATCATGGTCTTAATTTGGAGAAGGCTGGGGCTACAGCGGTTGTCCCAGAGACCTTGGAACCTAGTCTACAGTTGGCAGCTGCTGTCTTAGCTCAGGCTAAGCTACCCATGTCAGAGATCGCATCAACAATCAATGAGTATAGGTCCCGTCACCTTGGTGAGCTAACTGAGTTATGTGAAACTAGCGGAAGTTCTCTTGGCTACGGGTTTTCTCGAATGATGAGTAAACCCAAAACTCCGACTCCGGATTCCACAGATGAGAACCAGTTCACTGAAGGAACGCTGGCCATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008324 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010109 GO:0015075 GO:0015077 GO:0015078 GO:0015079 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015386 GO:0015491 GO:0015672 GO:0016020 GO:0016043 GO:0019222 GO:0022804 GO:0022821 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031323 GO:0031967 GO:0031969 GO:0031975 GO:0034220 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071804 GO:0071805 GO:0071840 GO:0098655 GO:0098660 GO:0098662 GO:0099516 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MutS_I MutS_II MutS_III MutS_V Na_H_Exchanger TrkA_N

Protein Analysis

1226

Amino Acids

132.59

Weight (kDa)

5.1

Isoelectric Point (pI)

37.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	631 - 997	7.3e-55	Sodium/hydrogen exchanger, transmembrane
TrkA_N	PF02254	1030 - 1143	1.4e-20	TrkA-N domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007831)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00630 AT4G00630
fragaria_vesca	FvH4_7g13660 FvH4_7g13660 FvH4_7g13660 FvH4_7g13660 FvH4_7g13660
malus_domestica	MD01G1053700.v1.1 MD07G1141400.v1.1
prunus_persica	Prupe.2G162700_v2.0.a1 Prupe.2G162700_v2.0.a1
pyrus_communis	pycom01g07930 pycom07g14030
rosa_chinensis	RchiOBHm_Chr1g0353741
rosa_laevigata	RLG00000028262
rosa_multiflora	Rmu_sc0005947.1_g000021
rosa_roxburghii	Rroxscaffold_4G00301700
rosa_rugosa	Rorug01G0237300 Rorug01G0237300
rosa_samantha	Rh1AG249500 Rh1BG219600 Rh1CG232200 Rh1DG246600
rosa_wichuraiana	Rw1G021770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	63
AasI	GACNNNNNNGTC	1 cut(s)	3215
AatII	GACGTC	1 cut(s)	1728
Acc36I	ACCTGC	2 cut(s)	2197, 2236
AccB1I	GGYRCC	1 cut(s)	493
AccB7I	CCANNNNNTGG	2 cut(s)	2320, 2675
AccBSI	CCGCTC	1 cut(s)	2260
AccI	GTMKAC	2 cut(s)	3219, 3451
AccII	CGCG	2 cut(s)	328, 608
AccIII	TCCGGA	1 cut(s)	3628
AciI	CCGC	5 cut(s)	328, 606, 2260, 3422, 3570
AclWI	GGATC	2 cut(s)	2717, 2770
AcoI	YGGCCR	7 cut(s)	906, 1628, 2284, 2448, 2973, 3115, 3669
AcsI	RAATTY	9 cut(s)	253, 810, 901, 1507, 1920, 2027, 2365, 2636, 2964
AcuI	CTGAAG	2 cut(s)	2424, 3678
AcyI	GRCGYC	1 cut(s)	1725
AfaI	GTAC	2 cut(s)	568, 2004
AfiI	CCNNNNNNNGG	5 cut(s)	1177, 1943, 2320, 2379, 2675
AflIII	ACRYGT	1 cut(s)	1869
AhlI	ACTAGT	1 cut(s)	1736
AjiI	CACGTC	1 cut(s)	2279
AjnI	CCWGG	8 cut(s)	873, 894, 1924, 2182, 2313, 2573, 2869, 3304
AjuI	GAANNNNNNNTTGG	4 cut(s)	666, 698, 2780, 2812
AleI	CACNNNNGTG	1 cut(s)	3540
Alw21I	GWGCWC	5 cut(s)	410, 1126, 2273, 3252, 3376
Alw26I	GTCTC	5 cut(s)	686, 788, 1211, 3059, 3428
AlwI	GGATC	2 cut(s)	2717, 2770
AlwNI	CAGNNNCTG	2 cut(s)	2633, 3467
Ama87I	CYCGRG	1 cut(s)	2063
Aor13HI	TCCGGA	1 cut(s)	3628
ApoI	RAATTY	9 cut(s)	253, 810, 901, 1507, 1920, 2027, 2365, 2636, 2964
Asp700I	GAANNNNTTC	2 cut(s)	711, 3650
AspA2I	CCTAGG	1 cut(s)	1228
AspS9I	GGNCC	6 cut(s)	1698, 1973, 2186, 2689, 3208, 3528
AsuC2I	CCSGG	1 cut(s)	2750
AsuII	TTCGAA	2 cut(s)	257, 3040
AsuNHI	GCTAGC	1 cut(s)	2989
AvaI	CYCGRG	1 cut(s)	2063
AvaII	GGWCC	3 cut(s)	2186, 3208, 3528
AvrII	CCTAGG	1 cut(s)	1228
BalI	TGGCCA	5 cut(s)	908, 1630, 2975, 3117, 3671
BanI	GGYRCC	1 cut(s)	493
BanII	GRGCYC	3 cut(s)	410, 1671, 3334
BbsI	GAAGAC	4 cut(s)	1353, 2555, 2672, 2849
Bbv12I	GWGCWC	5 cut(s)	410, 1126, 2273, 3252, 3376
BccI	CCATC	7 cut(s)	455, 821, 1274, 1568, 1600, 2450, 2984
BceAI	ACGGC	2 cut(s)	2271, 2463
BcgI	CGANNNNNNTGC	2 cut(s)	3020, 3054
BciT130I	CCWGG	8 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2871, 3306
BclI	TGATCA	2 cut(s)	1375, 3385
BcnI	CCSGG	1 cut(s)	2750
BcoDI	GTCTC	5 cut(s)	686, 788, 1211, 3059, 3428
BcuI	ACTAGT	1 cut(s)	1736
BfmI	CTRYAG	7 cut(s)	223, 2232, 2243, 3282, 3316, 3417, 3452
BfuAI	ACCTGC	2 cut(s)	2197, 2236
BglI	GCCNNNNNGGC	1 cut(s)	2447
BlnI	CCTAGG	1 cut(s)	1228
BlpI	GCTNAGC	1 cut(s)	3483
Bme1390I	CCNGG	9 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2750, 2871, 3306
Bme18I	GGWCC	3 cut(s)	2186, 3208, 3528
BmeT110I	CYCGRG	1 cut(s)	2063
BmgBI	CACGTC	1 cut(s)	2279
BmgT120I	GGNCC	6 cut(s)	1698, 1973, 2186, 2689, 3208, 3528
BmiI	GGNNCC	3 cut(s)	495, 3444, 3530
BmrFI	CCNGG	9 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2750, 2871, 3306
BmrI	ACTGGG	1 cut(s)	2696
BmsI	GCATC	7 cut(s)	421, 1090, 1562, 1971, 3023, 3220, 3515
BmtI	GCTAGC	1 cut(s)	2993
BmuI	ACTGGG	1 cut(s)	2696
BoxI	GACNNNNGTC	2 cut(s)	1223, 1353
BpiI	GAAGAC	4 cut(s)	1353, 2555, 2672, 2849
BplI	GAGNNNNNCTC	4 cut(s)	1653, 1685, 3234, 3266
BpmI	CTGGAG	2 cut(s)	1947, 3018
Bpu10I	CCTNAGC	3 cut(s)	409, 2124, 3477
Bpu1102I	GCTNAGC	1 cut(s)	3483
Bpu14I	TTCGAA	2 cut(s)	257, 3040
BpuEI	CTTGAG	3 cut(s)	1226, 1504, 1741
BpuMI	CCSGG	1 cut(s)	2750
BsaBI	GATNNNNATC	2 cut(s)	1194, 3384
BsaHI	GRCGYC	1 cut(s)	1725
BsaI	GGTCTC	2 cut(s)	788, 3428
BsaJI	CCNNGG	9 cut(s)	873, 1171, 1228, 1597, 2574, 2930, 2984, 3438, 3538
BsaWI	WCCGGW	1 cut(s)	3628
BsaXI	ACNNNNNCTCC	2 cut(s)	3610, 3640
Bsc4I	CCNNNNNNNGG	5 cut(s)	1177, 1943, 2320, 2379, 2675
Bse1I	ACTGG	4 cut(s)	2691, 3053, 3215, 3649
Bse3DI	GCAATG	3 cut(s)	1631, 2215, 2397
Bse8I	GATNNNNATC	2 cut(s)	1194, 3384
BseAI	TCCGGA	1 cut(s)	3628
BseBI	CCWGG	8 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2871, 3306
BseDI	CCNNGG	9 cut(s)	873, 1171, 1228, 1597, 2574, 2930, 2984, 3438, 3538
BseGI	GGATG	7 cut(s)	511, 628, 1033, 1653, 2225, 2369, 3076
BseJI	GATNNNNATC	2 cut(s)	1194, 3384
BseLI	CCNNNNNNNGG	5 cut(s)	1177, 1943, 2320, 2379, 2675
BseMI	GCAATG	3 cut(s)	1631, 2215, 2397
BseMII	CTCAG	9 cut(s)	423, 602, 1018, 1487, 2138, 3132, 3326, 3491, 3543
BseNI	ACTGG	4 cut(s)	2691, 3053, 3215, 3649
BseRI	GAGGAG	4 cut(s)	965, 1862, 2919, 3190
BseYI	CCCAGC	1 cut(s)	3410
BsgI	GTGCAG	1 cut(s)	537
Bsh1236I	CGCG	2 cut(s)	328, 608
BshNI	GGYRCC	1 cut(s)	493
BsiHKAI	GWGCWC	5 cut(s)	410, 1126, 2273, 3252, 3376
BsiHKCI	CYCGRG	1 cut(s)	2063
BsiSI	CCGG	2 cut(s)	2750, 3629
BslFI	GGGAC	5 cut(s)	1274, 1591, 2023, 3413, 3514
BslI	CCNNNNNNNGG	5 cut(s)	1177, 1943, 2320, 2379, 2675
BsmAI	GTCTC	5 cut(s)	686, 788, 1211, 3059, 3428
BsmBI	CGTCTC	2 cut(s)	1211, 3059
BsmFI	GGGAC	5 cut(s)	1274, 1591, 2023, 3413, 3514
BsmI	GAATGC	1 cut(s)	896
Bso31I	GGTCTC	2 cut(s)	788, 3428
BsoBI	CYCGRG	1 cut(s)	2063
Bsp119I	TTCGAA	2 cut(s)	257, 3040
Bsp1286I	GDGCHC	7 cut(s)	410, 1126, 1671, 2273, 3252, 3334, 3376
Bsp13I	TCCGGA	1 cut(s)	3628
Bsp1407I	TGTACA	1 cut(s)	2002
Bsp143I	GATC	7 cut(s)	241, 678, 1375, 2722, 2762, 3385, 3501
Bsp1720I	GCTNAGC	1 cut(s)	3483
Bsp19I	CCATGG	1 cut(s)	2984
BspACI	CCGC	5 cut(s)	328, 606, 2260, 3422, 3570
BspCNI	CTCAG	9 cut(s)	422, 601, 1017, 1486, 2137, 3133, 3327, 3490, 3544
BspEI	TCCGGA	1 cut(s)	3628
BspFNI	CGCG	2 cut(s)	328, 608
BspHI	TCATGA	5 cut(s)	397, 427, 685, 1378, 3376
BspLI	GGNNCC	3 cut(s)	495, 3444, 3530
BspMAI	CTGCAG	3 cut(s)	2236, 2247, 3286
BspMI	ACCTGC	2 cut(s)	2197, 2236
BspOI	GCTAGC	1 cut(s)	2993
BspPI	GGATC	2 cut(s)	2717, 2770
BspT104I	TTCGAA	2 cut(s)	257, 3040
BspT107I	GGYRCC	1 cut(s)	493
BspTNI	GGTCTC	2 cut(s)	788, 3428
BsrBI	CCGCTC	1 cut(s)	2260
BsrDI	GCAATG	3 cut(s)	1631, 2215, 2397
BsrGI	TGTACA	1 cut(s)	2002
BsrI	ACTGG	4 cut(s)	2691, 3053, 3215, 3649
BssECI	CCNNGG	9 cut(s)	873, 1171, 1228, 1597, 2574, 2930, 2984, 3438, 3538
BssMI	GATC	7 cut(s)	241, 678, 1375, 2722, 2762, 3385, 3501
BssNI	GRCGYC	1 cut(s)	1725
BssT1I	CCWWGG	6 cut(s)	1171, 1228, 1597, 2984, 3438, 3538
Bst2UI	CCWGG	8 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2871, 3306
Bst6I	CTCTTC	5 cut(s)	173, 684, 1017, 1570, 2699
BstACI	GRCGYC	1 cut(s)	1725
BstAPI	GCANNNNNTGC	6 cut(s)	635, 1109, 2251, 2438, 2891, 3281
BstAUI	TGTACA	1 cut(s)	2002
BstBI	TTCGAA	2 cut(s)	257, 3040
BstC8I	GCNNGC	6 cut(s)	1059, 1960, 2471, 2991, 3203, 3669
BstDSI	CCRYGG	1 cut(s)	2984
BstF5I	GGATG	7 cut(s)	511, 628, 1033, 1653, 2225, 2369, 3076
BstFNI	CGCG	2 cut(s)	328, 608
BstKTI	GATC	7 cut(s)	244, 681, 1378, 2725, 2765, 3388, 3504
BstMAI	GTCTC	5 cut(s)	686, 788, 1211, 3059, 3428
BstMBI	GATC	7 cut(s)	241, 678, 1375, 2722, 2762, 3385, 3501
BstNI	CCWGG	8 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2871, 3306
BstNSI	RCATGY	3 cut(s)	1094, 1873, 3279
BstPAI	GACNNNNGTC	2 cut(s)	1223, 1353
BstSCI	CCNGG	9 cut(s)	873, 894, 1924, 2182, 2313, 2573, 2748, 2869, 3304
BstSFI	CTRYAG	7 cut(s)	223, 2232, 2243, 3282, 3316, 3417, 3452
BstUI	CGCG	2 cut(s)	328, 608
BstV2I	GAAGAC	4 cut(s)	1353, 2555, 2672, 2849
BstXI	CCANNNNNNTGG	2 cut(s)	1147, 3260
BtgI	CCRYGG	1 cut(s)	2984
BtrI	CACGTC	1 cut(s)	2279
BtsCI	GGATG	7 cut(s)	511, 628, 1033, 1653, 2225, 2369, 3076
BtsI	GCAGTG	4 cut(s)	52, 771, 2229, 2568
BtsIMutI	CAGTG	6 cut(s)	52, 771, 1012, 2229, 2568, 3654
BveI	ACCTGC	2 cut(s)	2197, 2236
Cac8I	GCNNGC	6 cut(s)	1059, 1960, 2471, 2991, 3203, 3669
CaiI	CAGNNNCTG	2 cut(s)	2633, 3467
CciI	TCATGA	5 cut(s)	397, 427, 685, 1378, 3376
Cfr13I	GGNCC	6 cut(s)	1698, 1973, 2186, 2689, 3208, 3528
CseI	GACGC	4 cut(s)	1358, 1424, 2475, 3207
Csp6I	GTAC	2 cut(s)	567, 2003
CspCI	CAANNNNNGTGG	6 cut(s)	1428, 1463, 1723, 1758, 2218, 2253
CviQI	GTAC	2 cut(s)	567, 2003
DpnI	GATC	7 cut(s)	243, 680, 1377, 2724, 2764, 3387, 3503
DpnII	GATC	7 cut(s)	241, 678, 1375, 2722, 2762, 3385, 3501
DrdI	GACNNNNNNGTC	1 cut(s)	3215
DseDI	GACNNNNNNGTC	1 cut(s)	3215
EaeI	YGGCCR	7 cut(s)	906, 1628, 2284, 2448, 2973, 3115, 3669
Eam1104I	CTCTTC	5 cut(s)	173, 684, 1017, 1570, 2699
EarI	CTCTTC	5 cut(s)	173, 684, 1017, 1570, 2699
Ecl136II	GAGCTC	1 cut(s)	408
Eco130I	CCWWGG	6 cut(s)	1171, 1228, 1597, 2984, 3438, 3538
Eco24I	GRGCYC	3 cut(s)	410, 1671, 3334
Eco31I	GGTCTC	2 cut(s)	788, 3428
Eco32I	GATATC	1 cut(s)	1246
Eco47I	GGWCC	3 cut(s)	2186, 3208, 3528
Eco53kI	GAGCTC	1 cut(s)	408
Eco57I	CTGAAG	2 cut(s)	2424, 3678
Eco88I	CYCGRG	1 cut(s)	2063
EcoICRI	GAGCTC	1 cut(s)	408
EcoO109I	RGGNCCY	1 cut(s)	3528
EcoRI	GAATTC	2 cut(s)	253, 1920
EcoRII	CCWGG	8 cut(s)	873, 894, 1924, 2182, 2313, 2573, 2869, 3304
EcoRV	GATATC	1 cut(s)	1246
EcoT14I	CCWWGG	6 cut(s)	1171, 1228, 1597, 2984, 3438, 3538
EcoT38I	GRGCYC	3 cut(s)	410, 1671, 3334
ErhI	CCWWGG	6 cut(s)	1171, 1228, 1597, 2984, 3438, 3538
Esp3I	CGTCTC	2 cut(s)	1211, 3059
FalI	AAGNNNNNCTT	2 cut(s)	1845, 1877
FaqI	GGGAC	5 cut(s)	1274, 1591, 2023, 3413, 3514
FauI	CCCGC	1 cut(s)	2253
FbaI	TGATCA	2 cut(s)	1375, 3385
FblI	GTMKAC	2 cut(s)	3219, 3451
FokI	GGATG	7 cut(s)	518, 635, 1040, 1660, 2212, 2356, 3083
FriOI	GRGCYC	3 cut(s)	410, 1671, 3334
GsaI	CCCAGC	1 cut(s)	3414
GsuI	CTGGAG	2 cut(s)	1947, 3018
HapII	CCGG	2 cut(s)	2750, 3629
HgaI	GACGC	4 cut(s)	1358, 1424, 2475, 3207
Hin1I	GRCGYC	1 cut(s)	1725
HincII	GTYRAC	3 cut(s)	652, 1096, 1153
HindII	GTYRAC	3 cut(s)	652, 1096, 1153
HindIII	AAGCTT	1 cut(s)	1854
HpaI	GTTAAC	1 cut(s)	652
HpaII	CCGG	2 cut(s)	2750, 3629
HpyCH4IV	ACGT	7 cut(s)	214, 1422, 1725, 2278, 2294, 3036, 3354
HpySE526I	ACGT	7 cut(s)	214, 1422, 1725, 2278, 2294, 3036, 3354
Hsp92I	GRCGYC	1 cut(s)	1725
Kpn2I	TCCGGA	1 cut(s)	3628
Ksp22I	TGATCA	2 cut(s)	1375, 3385
KspAI	GTTAAC	1 cut(s)	652
Kzo9I	GATC	7 cut(s)	241, 678, 1375, 2722, 2762, 3385, 3501
LmnI	GCTCC	4 cut(s)	2600, 2671, 2872, 3255
LweI	GCATC	7 cut(s)	421, 1090, 1562, 1971, 3023, 3220, 3515
MaeII	ACGT	7 cut(s)	214, 1422, 1725, 2278, 2294, 3036, 3354
MaeIII	GTNAC	6 cut(s)	122, 281, 1030, 1710, 2134, 3533
MalI	GATC	7 cut(s)	243, 680, 1377, 2724, 2764, 3387, 3503
MbiI	CCGCTC	1 cut(s)	2260
MboI	GATC	7 cut(s)	241, 678, 1375, 2722, 2762, 3385, 3501
MfeI	CAATTG	1 cut(s)	2019
MhlI	GDGCHC	7 cut(s)	410, 1126, 1671, 2273, 3252, 3334, 3376
MlsI	TGGCCA	5 cut(s)	908, 1630, 2975, 3117, 3671
MluNI	TGGCCA	5 cut(s)	908, 1630, 2975, 3117, 3671
MlyI	GAGTC	6 cut(s)	338, 1573, 2663, 2920, 3154, 3619
MmeI	TCCRAC	6 cut(s)	1164, 1607, 2689, 2941, 3175, 3647
Mox20I	TGGCCA	5 cut(s)	908, 1630, 2975, 3117, 3671
MroI	TCCGGA	1 cut(s)	3628
MroXI	GAANNNNTTC	2 cut(s)	711, 3650
MscI	TGGCCA	5 cut(s)	908, 1630, 2975, 3117, 3671
MslI	CAYNNNNRTG	4 cut(s)	1383, 2004, 2286, 3540
Msp20I	TGGCCA	5 cut(s)	908, 1630, 2975, 3117, 3671
MspA1I	CMGCKG	3 cut(s)	2141, 3422, 3464
MspI	CCGG	2 cut(s)	2750, 3629
MspR9I	CCNGG	9 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2750, 2871, 3306
MunI	CAATTG	1 cut(s)	2019
Mva1269I	GAATGC	1 cut(s)	896
MvaI	CCWGG	8 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2871, 3306
MvnI	CGCG	2 cut(s)	328, 608
NciI	CCSGG	1 cut(s)	2750
NcoI	CCATGG	1 cut(s)	2984
NdeII	GATC	7 cut(s)	241, 678, 1375, 2722, 2762, 3385, 3501
NheI	GCTAGC	1 cut(s)	2989
NlaIV	GGNNCC	3 cut(s)	495, 3444, 3530
NmeAIII	GCCGAG	2 cut(s)	460, 3266
NmuCI	GTSAC	2 cut(s)	2134, 3533
NspI	RCATGY	3 cut(s)	1094, 1873, 3279
NspV	TTCGAA	2 cut(s)	257, 3040
OliI	CACNNNNGTG	1 cut(s)	3540
PaeR7I	CTCGAG	1 cut(s)	2063
PagI	TCATGA	5 cut(s)	397, 427, 685, 1378, 3376
PciI	ACATGT	1 cut(s)	1869
PcsI	WCGNNNNNNNCGW	1 cut(s)	2284
PctI	GAATGC	1 cut(s)	896
PdmI	GAANNNNTTC	2 cut(s)	711, 3650
PfeI	GAWTC	7 cut(s)	259, 446, 770, 1403, 1825, 3299, 3632
PflMI	CCANNNNNTGG	2 cut(s)	2320, 2675
PfoI	TCCNGGA	2 cut(s)	1924, 2313
PleI	GAGTC	6 cut(s)	338, 1573, 2662, 2920, 3153, 3619
PpsI	GAGTC	6 cut(s)	338, 1573, 2662, 2920, 3153, 3619
PpuMI	RGGWCCY	1 cut(s)	3528
PscI	ACATGT	1 cut(s)	1869
PshAI	GACNNNNGTC	2 cut(s)	1223, 1353
PsiI	TTATAA	1 cut(s)	63
Psp124BI	GAGCTC	1 cut(s)	410
Psp5II	RGGWCCY	1 cut(s)	3528
Psp6I	CCWGG	8 cut(s)	873, 894, 1924, 2182, 2313, 2573, 2869, 3304
PspFI	CCCAGC	1 cut(s)	3410
PspGI	CCWGG	8 cut(s)	873, 894, 1924, 2182, 2313, 2573, 2869, 3304
PspN4I	GGNNCC	3 cut(s)	495, 3444, 3530
PspPI	GGNCC	6 cut(s)	1698, 1973, 2186, 2689, 3208, 3528
PspPPI	RGGWCCY	1 cut(s)	3528
PspXI	VCTCGAGB	1 cut(s)	2063
PsrI	GAACNNNNNNTAC	4 cut(s)	1480, 1512, 3435, 3467
PstI	CTGCAG	3 cut(s)	2236, 2247, 3286
PstNI	CAGNNNCTG	2 cut(s)	2633, 3467
PvuII	CAGCTG	2 cut(s)	2141, 3464
RsaI	GTAC	2 cut(s)	568, 2004
RsaNI	GTAC	2 cut(s)	567, 2003
RseI	CAYNNNNRTG	4 cut(s)	1383, 2004, 2286, 3540
SacI	GAGCTC	1 cut(s)	410
Sau3AI	GATC	7 cut(s)	241, 678, 1375, 2722, 2762, 3385, 3501
Sau96I	GGNCC	6 cut(s)	1698, 1973, 2186, 2689, 3208, 3528
SchI	GAGTC	6 cut(s)	338, 1573, 2663, 2920, 3154, 3619
ScrFI	CCNGG	9 cut(s)	875, 896, 1926, 2184, 2315, 2575, 2750, 2871, 3306
SduI	GDGCHC	7 cut(s)	410, 1126, 1671, 2273, 3252, 3334, 3376
SfaNI	GCATC	7 cut(s)	421, 1090, 1562, 1971, 3023, 3220, 3515
SfcI	CTRYAG	7 cut(s)	223, 2232, 2243, 3282, 3316, 3417, 3452
Sfr274I	CTCGAG	1 cut(s)	2063
SfuI	TTCGAA	2 cut(s)	257, 3040
SinI	GGWCC	3 cut(s)	2186, 3208, 3528
SlaI	CTCGAG	1 cut(s)	2063
SmiMI	CAYNNNNRTG	4 cut(s)	1383, 2004, 2286, 3540
SmlI	CTYRAG	4 cut(s)	1205, 1483, 1756, 2063
SmoI	CTYRAG	4 cut(s)	1205, 1483, 1756, 2063
SpeI	ACTAGT	1 cut(s)	1736
SsiI	CCGC	5 cut(s)	328, 606, 2260, 3422, 3570
SspI	AATATT	1 cut(s)	2523
SstI	GAGCTC	1 cut(s)	410
StyD4I	CCNGG	9 cut(s)	873, 894, 1924, 2182, 2313, 2573, 2748, 2869, 3304
StyI	CCWWGG	6 cut(s)	1171, 1228, 1597, 2984, 3438, 3538
TaiI	ACGT	7 cut(s)	217, 1425, 1728, 2281, 2297, 3039, 3357
TaqI	TCGA	9 cut(s)	140, 257, 1687, 1777, 2064, 2479, 3040, 3057, 3598
TatI	WGTACW	1 cut(s)	2002
TauI	GCSGC	1 cut(s)	608
TfiI	GAWTC	7 cut(s)	259, 446, 770, 1403, 1825, 3299, 3632
TscAI	CASTG	6 cut(s)	52, 771, 1012, 2236, 2575, 3661
TseFI	GTSAC	2 cut(s)	2134, 3533
Tsp45I	GTSAC	2 cut(s)	2134, 3533
TspGWI	ACGGA	2 cut(s)	3083, 3359
TspRI	CASTG	6 cut(s)	52, 771, 1012, 2236, 2575, 3661
Van91I	CCANNNNNTGG	2 cut(s)	2320, 2675
VpaK11BI	GGWCC	3 cut(s)	2186, 3208, 3528
XapI	RAATTY	9 cut(s)	253, 810, 901, 1507, 1920, 2027, 2365, 2636, 2964
XceI	RCATGY	3 cut(s)	1094, 1873, 3279
XhoI	CTCGAG	1 cut(s)	2063
XmaJI	CCTAGG	1 cut(s)	1228
XmiI	GTMKAC	2 cut(s)	3219, 3451
XmnI	GAANNNNTTC	2 cut(s)	711, 3650
ZraI	GACGTC	1 cut(s)	1726

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.