MD01G1055200.v1.1

O-glucosyltransferase rumi homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004904
Physical Location & Seq
Forward (+)
15978768 .. 15981585
2818 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1055200.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGAAGAGGAACAATGGGAAGCATTTGTATGAGTTGTTGGATGGGACAGGATTGCATCGGCATTTTATAGGAACAAGTTGGAGGCCATTGCAGAAGAGAAGTGCAACAGCTTCTTTAATCTTCTTCCTGTTGTTGCTGCTTTTGTGTGTTGCTGCGTTGTTATCTGCTGGCTGGATTGATACTAGAAAGTTTATATTTTCAGGTACTTATTCTAACGAATCAATCATACCCACAAAAGAAACCCCAAAAAGAATTGAATTCCCTCTGCAGTGTACCAAAGGAATTAATGTAACACAAACTTGTCCAAGAACCTACCCTACCACACACAACCCCACAAATCCTAACCGTCCATCAAACCTCACATGCCCGTCACACTTCCGATGGATCCATGAAGATCTAAGACCATGGAAAGGGACGGGAATCACAAGGGACATGATTGAGCGGGCCCGAAAGACTGCACATTTTAGGCTTGTGATCGTGGACGGAAAGGCCTACATAGAGAAGTACAGACAATCCATACAAACTAGGGACATGTTCACGTTATGGGGCATTTTACAGCTTCTAAGGGTTTACCCTGGTAGATTGCCTGACTTGGAGCTCATGTTTGACTGCGATGATCGGCCTGTCGTCCGATCAAAGGACTTTCGGGGGCCTAATGTTGGCCCGCCACCGGTGTTTCGATATTGTGCAGACGAGGAGAGCTTGGACATTGTGTTTCCAGATTGGTCCTTTTGGGGCTGGGTTGAGATCAACATAAAACCATGGAGAAGTTTATTGCCAAGCATAAAAGAAGGCAATAAAAGAACAAAGTGGAAGGACAGGATCCCCTATGCTTACTGGAAAGGTAACCCTCACGTCGCTCCAACCAGAAAGGACCTTCTCAAATGCAATGTCTCAGAAAAAAATGACTGGAATACACGCCTATATATACAGGATTGGGAGAAAGAATCTAAACAAGGGTACAAGCAATCCAACTTGGAAGACCAGTGCAAGCATAGGTATAAAATTTATGTAGAAGGATGGGCGTGGTCTGTAAGTGAAAAATACATCATGGCATGCAATTCTATGACGCTGTATGTAAAGCCTCGGTACTACGATTTCTTTATGAGAAGCATGACGCCATTGCAGCATTACTGGCCTATTAGGGACAACAGCAAGTGCACTTCTCTTAAGTTCGCGGTGGAATGGGGCAATAATCACACAGACAAGGTGCAGGAAATTGGGGAGGCAGCTAGCAAATTCATACAAGAAGATCTAAAGATGGACTATGTGTACGATTACATGTTTCATGTGCTAAATGAATATGCAAAGCTCTTGCAATTCAAAGCGACTATACCTCCCAGTGCAGTGGAGCTATGCTCAGAAACAATGGCATGTGCTGCAAATGGTACATGGAAGAACTTCATGGTGGAGTCCATGGTGAAGTATCCTAGTGATGAACTGCCATGCACTCTGCCTCCTCCATACGAATCTCCGGCTCTTCGTAATTTTCTTGAGAGAAAGGCTAATTCAACTAGGCAAGTAGAAGTTTGGGAAGATGAATACTGGAAAAGTAGAAATAAAACGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
Glyco_transf_90

Protein Analysis

524

Amino Acids

61.6

Weight (kDa)

9.12

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_90 PF05686 119 - 512 1.4e-195 Glycosyl transferase family 90
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 442
AccII CGCG 1 cut(s) 1178
AciI CCGC 3 cut(s) 442, 665, 1178
AclWI GGATC 4 cut(s) 379, 392, 817, 830
AcsI RAATTY 3 cut(s) 257, 1005, 1238
AcyI GRCGYC 1 cut(s) 1118
AfaI GTAC 7 cut(s) 205, 274, 506, 962, 1091, 1274, 1390
AfiI CCNNNNNNNGG 4 cut(s) 410, 637, 659, 670
AflII CTTAAG 1 cut(s) 1169
AflIII ACRYGT 2 cut(s) 531, 1281
AgeI ACCGGT 1 cut(s) 670
AgsI TTSAA 3 cut(s) 257, 1324, 1512
AjiI CACGTC 1 cut(s) 856
AjnI CCWGG 1 cut(s) 574
AluBI AGCT 7 cut(s) 110, 559, 598, 702, 1232, 1312, 1354
AluI AGCT 7 cut(s) 110, 559, 598, 702, 1232, 1312, 1354
Alw21I GWGCWC 2 cut(s) 600, 1163
Alw26I GTCTC 1 cut(s) 898
Alw44I GTGCAC 1 cut(s) 1159
AlwI GGATC 4 cut(s) 379, 392, 817, 830
AoxI GGCC 7 cut(s) 83, 444, 489, 620, 650, 661, 1136
ApaI GGGCCC 1 cut(s) 448
ApaLI GTGCAC 1 cut(s) 1159
ApeKI GCWGC 5 cut(s) 136, 152, 1126, 1229, 1379
ApoI RAATTY 3 cut(s) 257, 1005, 1238
ArsI GACNNNNNNTTYG 2 cut(s) 876, 908
AseI ATTAAT 1 cut(s) 285
AsiGI ACCGGT 1 cut(s) 670
Asp700I GAANNNNTTC 1 cut(s) 1400
AspS9I GGNCC 6 cut(s) 444, 445, 650, 662, 726, 874
AsuHPI GGTGA 1 cut(s) 1432
AsuNHI GCTAGC 1 cut(s) 1232
AvaII GGWCC 2 cut(s) 726, 874
BaeGI GKGCMC 2 cut(s) 448, 1163
BamHI GGATCC 2 cut(s) 384, 822
BanII GRGCYC 2 cut(s) 448, 600
BbsI GAAGAC 1 cut(s) 987
Bbv12I GWGCWC 2 cut(s) 600, 1163
BbvI GCAGC 5 cut(s) 123, 139, 1138, 1241, 1366
BccI CCATC 5 cut(s) 35, 358, 375, 1014, 1255
BciT130I CCWGG 1 cut(s) 576
BciVI GTATCC 1 cut(s) 1437
BcoDI GTCTC 1 cut(s) 898
BfaI CTAG 5 cut(s) 183, 525, 1233, 1431, 1515
BfmI CTRYAG 1 cut(s) 266
BfrI CTTAAG 1 cut(s) 1169
BfuI GTATCC 1 cut(s) 1437
BglII AGATCT 2 cut(s) 394, 1252
BisI GCNGC 5 cut(s) 137, 153, 1127, 1230, 1380
BlsI GCNGC 5 cut(s) 138, 154, 1128, 1231, 1381
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 726, 874
BmgBI CACGTC 1 cut(s) 856
BmgT120I GGNCC 6 cut(s) 444, 445, 650, 662, 726, 874
BmiI GGNNCC 4 cut(s) 386, 446, 651, 824
BmrFI CCNGG 1 cut(s) 576
BmrI ACTGGG 1 cut(s) 1335
BmsI GCATC 1 cut(s) 64
BmtI GCTAGC 1 cut(s) 1236
BmuI ACTGGG 1 cut(s) 1335
BpiI GAAGAC 1 cut(s) 987
BplI GAGNNNNNCTC 2 cut(s) 1343, 1375
BpuEI CTTGAG 1 cut(s) 1514
BsaHI GRCGYC 1 cut(s) 1118
BsaJI CCNNGG 5 cut(s) 404, 574, 761, 1085, 1416
BsaWI WCCGGW 1 cut(s) 670
BsaXI ACNNNNNCTCC 6 cut(s) 587, 617, 1321, 1351, 1441, 1471
Bsc4I CCNNNNNNNGG 4 cut(s) 410, 637, 659, 670
Bse118I RCCGGY 1 cut(s) 670
Bse1I ACTGG 6 cut(s) 842, 914, 985, 1139, 1341, 1550
Bse3DI GCAATG 3 cut(s) 86, 895, 1121
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 5 cut(s) 404, 574, 761, 1085, 1416
BseGI GGATG 2 cut(s) 46, 1025
BseLI CCNNNNNNNGG 4 cut(s) 410, 637, 659, 670
BseMI GCAATG 3 cut(s) 86, 895, 1121
BseMII CTCAG 2 cut(s) 909, 1374
BseNI ACTGG 6 cut(s) 842, 914, 985, 1139, 1341, 1550
BseRI GAGGAG 2 cut(s) 710, 1449
BseSI GKGCMC 2 cut(s) 448, 1163
BseXI GCAGC 5 cut(s) 123, 139, 1138, 1241, 1366
BseYI CCCAGC 1 cut(s) 738
BsgI GTGCAG 4 cut(s) 441, 708, 1232, 1365
Bsh1236I CGCG 1 cut(s) 1178
BshFI GGCC 7 cut(s) 85, 446, 491, 622, 652, 663, 1138
BshTI ACCGGT 1 cut(s) 670
BsiHKAI GWGCWC 2 cut(s) 600, 1163
BsiSI CCGG 2 cut(s) 671, 1475
BslFI GGGAC 5 cut(s) 58, 427, 443, 542, 1160
BslI CCNNNNNNNGG 4 cut(s) 410, 637, 659, 670
BsmAI GTCTC 1 cut(s) 898
BsmFI GGGAC 5 cut(s) 58, 427, 443, 542, 1160
BsnI GGCC 7 cut(s) 85, 446, 491, 622, 652, 663, 1138
Bsp120I GGGCCC 1 cut(s) 444
Bsp1286I GDGCHC 3 cut(s) 448, 600, 1163
Bsp143I GATC 8 cut(s) 384, 394, 474, 616, 632, 747, 822, 1252
Bsp19I CCATGG 3 cut(s) 404, 761, 1416
BspACI CCGC 3 cut(s) 442, 665, 1178
BspANI GGCC 7 cut(s) 85, 446, 491, 622, 652, 663, 1138
BspCNI CTCAG 2 cut(s) 908, 1373
BspFNI CGCG 1 cut(s) 1178
BspLI GGNNCC 4 cut(s) 386, 446, 651, 824
BspMAI CTGCAG 1 cut(s) 270
BspOI GCTAGC 1 cut(s) 1236
BspPI GGATC 4 cut(s) 379, 392, 817, 830
BspQI GCTCTTC 1 cut(s) 1485
BspTI CTTAAG 1 cut(s) 1169
BsrBI CCGCTC 1 cut(s) 442
BsrDI GCAATG 3 cut(s) 86, 895, 1121
BsrFI RCCGGY 1 cut(s) 670
BsrI ACTGG 6 cut(s) 842, 914, 985, 1139, 1341, 1550
BssAI RCCGGY 1 cut(s) 670
BssECI CCNNGG 5 cut(s) 404, 574, 761, 1085, 1416
BssMI GATC 8 cut(s) 384, 394, 474, 616, 632, 747, 822, 1252
BssNI GRCGYC 1 cut(s) 1118
BssT1I CCWWGG 3 cut(s) 404, 761, 1416
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 1 cut(s) 347
Bst6I CTCTTC 2 cut(s) 89, 1485
BstACI GRCGYC 1 cut(s) 1118
BstAFI CTTAAG 1 cut(s) 1169
BstAPI GCANNNNNTGC 1 cut(s) 1379
BstC8I GCNNGC 6 cut(s) 169, 444, 665, 992, 1057, 1234
BstDEI CTNAG 4 cut(s) 398, 563, 895, 1360
BstDSI CCRYGG 3 cut(s) 404, 761, 1416
BstEII GGTNACC 1 cut(s) 845
BstF5I GGATG 2 cut(s) 46, 1025
BstFNI CGCG 1 cut(s) 1178
BstKTI GATC 8 cut(s) 387, 397, 477, 619, 635, 750, 825, 1255
BstMAI GTCTC 1 cut(s) 898
BstMBI GATC 8 cut(s) 384, 394, 474, 616, 632, 747, 822, 1252
BstMWI GCNNNNNNNGC 3 cut(s) 1126, 1135, 1379
BstNI CCWGG 1 cut(s) 576
BstNSI RCATGY 5 cut(s) 366, 535, 1059, 1285, 1377
BstPI GGTNACC 1 cut(s) 845
BstSCI CCNGG 1 cut(s) 574
BstSFI CTRYAG 1 cut(s) 266
BstSLI GKGCMC 2 cut(s) 448, 1163
BstUI CGCG 1 cut(s) 1178
BstV1I GCAGC 5 cut(s) 123, 139, 1138, 1241, 1366
BstV2I GAAGAC 1 cut(s) 987
BstX2I RGATCY 4 cut(s) 384, 394, 822, 1252
BstXI CCANNNNNNTGG 1 cut(s) 1348
BstYI RGATCY 4 cut(s) 384, 394, 822, 1252
BsuI GTATCC 1 cut(s) 1437
BsuRI GGCC 7 cut(s) 85, 446, 491, 622, 652, 663, 1138
BtgI CCRYGG 3 cut(s) 404, 761, 1416
BtgZI GCGATG 1 cut(s) 627
BtrI CACGTC 1 cut(s) 856
BtsCI GGATG 2 cut(s) 46, 1025
BtsI GCAGTG 2 cut(s) 275, 1353
BtsIMutI CAGTG 4 cut(s) 275, 992, 1348, 1353
Cac8I GCNNGC 6 cut(s) 169, 444, 665, 992, 1057, 1234
Cfr10I RCCGGY 1 cut(s) 670
Cfr13I GGNCC 6 cut(s) 444, 445, 650, 662, 726, 874
CseI GACGC 2 cut(s) 1078, 1126
Csp6I GTAC 7 cut(s) 204, 273, 505, 961, 1090, 1273, 1389
CspAI ACCGGT 1 cut(s) 670
CviQI GTAC 7 cut(s) 204, 273, 505, 961, 1090, 1273, 1389
DdeI CTNAG 4 cut(s) 398, 563, 895, 1360
DpnI GATC 8 cut(s) 386, 396, 476, 618, 634, 749, 824, 1254
DpnII GATC 8 cut(s) 384, 394, 474, 616, 632, 747, 822, 1252
Eam1104I CTCTTC 2 cut(s) 89, 1485
EarI CTCTTC 2 cut(s) 89, 1485
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 3 cut(s) 404, 761, 1416
Eco147I AGGCCT 1 cut(s) 491
Eco24I GRGCYC 2 cut(s) 448, 600
Eco47I GGWCC 2 cut(s) 726, 874
Eco53kI GAGCTC 1 cut(s) 598
Eco91I GGTNACC 1 cut(s) 845
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 2 cut(s) 650, 874
EcoO65I GGTNACC 1 cut(s) 845
EcoRI GAATTC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 574
EcoT14I CCWWGG 3 cut(s) 404, 761, 1416
EcoT38I GRGCYC 2 cut(s) 448, 600
ErhI CCWWGG 3 cut(s) 404, 761, 1416
FaqI GGGAC 5 cut(s) 58, 427, 443, 542, 1160
FauI CCCGC 2 cut(s) 435, 672
Fnu4HI GCNGC 5 cut(s) 137, 153, 1127, 1230, 1380
FokI GGATG 2 cut(s) 53, 1032
FriOI GRGCYC 2 cut(s) 448, 600
Fsp4HI GCNGC 5 cut(s) 137, 153, 1127, 1230, 1380
FspBI CTAG 5 cut(s) 183, 525, 1233, 1431, 1515
GluI GCNGC 5 cut(s) 137, 153, 1127, 1230, 1380
GsaI CCCAGC 1 cut(s) 742
HaeIII GGCC 7 cut(s) 85, 446, 491, 622, 652, 663, 1138
HapII CCGG 2 cut(s) 671, 1475
HgaI GACGC 2 cut(s) 1078, 1126
Hin1I GRCGYC 1 cut(s) 1118
HinfI GANTC 5 cut(s) 218, 420, 947, 1412, 1469
HpaII CCGG 2 cut(s) 671, 1475
HphI GGTGA 1 cut(s) 1432
Hpy166II GTNNAC 6 cut(s) 273, 481, 537, 571, 1161, 1273
Hpy188I TCNGA 4 cut(s) 380, 632, 898, 1363
Hpy188III TCNNGA 2 cut(s) 719, 1493
Hpy8I GTNNAC 6 cut(s) 273, 481, 537, 571, 1161, 1273
Hpy99I CGWCG 1 cut(s) 860
HpyAV CCTTC 4 cut(s) 785, 808, 887, 1010
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4IV ACGT 2 cut(s) 539, 855
HpyF10VI GCNNNNNNNGC 3 cut(s) 1126, 1135, 1379
HpyF3I CTNAG 4 cut(s) 398, 563, 895, 1360
HpySE526I ACGT 2 cut(s) 539, 855
Hsp92I GRCGYC 1 cut(s) 1118
Kzo9I GATC 8 cut(s) 384, 394, 474, 616, 632, 747, 822, 1252
LguI GCTCTTC 1 cut(s) 1485
LmnI GCTCC 3 cut(s) 595, 865, 1351
Lsp1109I GCAGC 5 cut(s) 123, 139, 1138, 1241, 1366
LweI GCATC 1 cut(s) 64
MaeI CTAG 5 cut(s) 183, 525, 1233, 1431, 1515
MaeII ACGT 2 cut(s) 539, 855
MaeIII GTNAC 3 cut(s) 289, 369, 845
MalI GATC 8 cut(s) 386, 396, 476, 618, 634, 749, 824, 1254
MbiI CCGCTC 1 cut(s) 442
MboI GATC 8 cut(s) 384, 394, 474, 616, 632, 747, 822, 1252
MflI RGATCY 4 cut(s) 384, 394, 822, 1252
MhlI GDGCHC 3 cut(s) 448, 600, 1163
MlyI GAGTC 1 cut(s) 1421
MmeI TCCRAC 4 cut(s) 18, 59, 887, 996
MroXI GAANNNNTTC 1 cut(s) 1400
MseI TTAA 3 cut(s) 116, 285, 1170
MslI CAYNNNNRTG 1 cut(s) 27
MspCI CTTAAG 1 cut(s) 1169
MspI CCGG 2 cut(s) 671, 1475
MspR9I CCNGG 1 cut(s) 576
MvaI CCWGG 1 cut(s) 576
MvnI CGCG 1 cut(s) 1178
MwoI GCNNNNNNNGC 3 cut(s) 1126, 1135, 1379
NcoI CCATGG 3 cut(s) 404, 761, 1416
NdeII GATC 8 cut(s) 384, 394, 474, 616, 632, 747, 822, 1252
NheI GCTAGC 1 cut(s) 1232
NlaIV GGNNCC 4 cut(s) 386, 446, 651, 824
NmuCI GTSAC 1 cut(s) 369
NspI RCATGY 5 cut(s) 366, 535, 1059, 1285, 1377
PaeI GCATGC 1 cut(s) 1059
PceI AGGCCT 1 cut(s) 491
PciI ACATGT 2 cut(s) 531, 1281
PciSI GCTCTTC 1 cut(s) 1485
PdmI GAANNNNTTC 1 cut(s) 1400
PfeI GAWTC 4 cut(s) 218, 420, 947, 1469
PinAI ACCGGT 1 cut(s) 670
PkrI GCNGC 5 cut(s) 138, 154, 1128, 1231, 1381
PleI GAGTC 1 cut(s) 1420
PpsI GAGTC 1 cut(s) 1420
PpuMI RGGWCCY 1 cut(s) 874
PscI ACATGT 2 cut(s) 531, 1281
PshBI ATTAAT 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 874
Psp6I CCWGG 1 cut(s) 574
PspEI GGTNACC 1 cut(s) 845
PspFI CCCAGC 1 cut(s) 738
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 4 cut(s) 386, 446, 651, 824
PspOMI GGGCCC 1 cut(s) 444
PspPI GGNCC 6 cut(s) 444, 445, 650, 662, 726, 874
PspPPI RGGWCCY 1 cut(s) 874
PsrI GAACNNNNNNTAC 2 cut(s) 301, 333
PstI CTGCAG 1 cut(s) 270
PsuI RGATCY 4 cut(s) 384, 394, 822, 1252
RsaI GTAC 7 cut(s) 205, 274, 506, 962, 1091, 1274, 1390
RsaNI GTAC 7 cut(s) 204, 273, 505, 961, 1090, 1273, 1389
RseI CAYNNNNRTG 1 cut(s) 27
SacI GAGCTC 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 1485
SaqAI TTAA 3 cut(s) 116, 285, 1170
SatI GCNGC 5 cut(s) 137, 153, 1127, 1230, 1380
Sau3AI GATC 8 cut(s) 384, 394, 474, 616, 632, 747, 822, 1252
Sau96I GGNCC 6 cut(s) 444, 445, 650, 662, 726, 874
SchI GAGTC 1 cut(s) 1421
ScrFI CCNGG 1 cut(s) 576
SduI GDGCHC 3 cut(s) 448, 600, 1163
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 1 cut(s) 266
SgrAI CRCCGGYG 1 cut(s) 670
SinI GGWCC 2 cut(s) 726, 874
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 1169, 1493
SmoI CTYRAG 2 cut(s) 1169, 1493
SphI GCATGC 1 cut(s) 1059
SseBI AGGCCT 1 cut(s) 491
SsiI CCGC 3 cut(s) 442, 665, 1178
SspMI CTAG 5 cut(s) 183, 525, 1233, 1431, 1515
SstI GAGCTC 1 cut(s) 600
StuI AGGCCT 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 574
StyI CCWWGG 3 cut(s) 404, 761, 1416
TaaI ACNGT 1 cut(s) 347
TaiI ACGT 2 cut(s) 542, 858
TaqI TCGA 1 cut(s) 679
TatI WGTACW 1 cut(s) 504
TfiI GAWTC 4 cut(s) 218, 420, 947, 1469
Tru1I TTAA 3 cut(s) 116, 285, 1170
Tru9I TTAA 3 cut(s) 116, 285, 1170
TscAI CASTG 4 cut(s) 275, 992, 1348, 1353
TseFI GTSAC 1 cut(s) 369
TseI GCWGC 5 cut(s) 136, 152, 1126, 1229, 1379
Tsp45I GTSAC 1 cut(s) 369
TspDTI ATGAA 8 cut(s) 17, 405, 1231, 1277, 1314, 1393, 1452, 1554
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 4 cut(s) 275, 992, 1348, 1353
Vha464I CTTAAG 1 cut(s) 1169
VneI GTGCAC 1 cut(s) 1159
VpaK11BI GGWCC 2 cut(s) 726, 874
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 3 cut(s) 257, 1005, 1238
XceI RCATGY 5 cut(s) 366, 535, 1059, 1285, 1377
XmnI GAANNNNTTC 1 cut(s) 1400
XspI CTAG 5 cut(s) 183, 525, 1233, 1431, 1515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.