RLG00000028227

O-glucosyltransferase rumi homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004904
Physical Location & Seq
Reverse (-)
20322404 .. 20325145
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028227

Sequence Viewer

Length: 1575 bp
ATGAAAAGGAACAATGCGAAGCATTTGTATGATTTCTTGGACGGGTCAGGGTTGCATAGGCATTTTGCAGGATCAAGTTGGCAGCCATCGAAGAAGAGAACCGCGACATTTACAACTCTAGCCTTCTTGTTCTTGTTGTTCTTCTTTGTTGCTGCTCTGGCATTTGCCGGGTGGAGTGTTGATATTGACTCTTTTATATTTTCAGGTGCTTCTTCTAACAAATCAATCCCAACACCAACAACAATACCCAAAACCATTGAATTCCCACTGCAATGTGCCACAGGAATTAATGTGACACAAACTTGTCCAAGAATCTACCCTAGCAAACACAACCCTACAAGTCCTAACCTTCCATCAAATGTCACATGCCCATCATACTTCCGATGGATCCACGAAGATCTACGGCCATGGAGAGAGACAGGAATCACAAGGGACATGGTGGAGCAGGCGCGCAGGACTGCACATTTTAGGCTCATGATCGTGGATGGCAAGGCCTACCTGGAGAAGTATAGGCAGTCCATACAAACTAGGGACATGTTTACATTATGGGGCATTTTGCAGCTTCTAAGGGTATACCCTGGTAGATTGCCTGACTTGGAGATCATGTTTGACTGTGATGATCGGCCTGTCGTCAGGTCAAGAGACTTCCGGGAACCAAATGCTGGCCCTCCGCCGGTTTTCCGATACTGTGCCGATGAAGGCAGCTTGGACATTGTGTTCCCAGATTGGTCTTTTTGGGGCTGGGCTGAGACCAATATAAAGCCATGGAAAAACTTATTGCCAAGCATAAAGGAAGGCAATAAAAGAACGAAGTGGAAGGATAGGGTGCCCTATGCTTACTGGAAAGGTAACCCAAATGTGGCTCCAACCAGGAAAGACCTTCTCAAATGCAATGTTTCAGACCAAAATGACTGGAATACACTTCTATACATACAGAATTGGGAGAAAGAATCCAAAGAAGGGTACAAGGAGTCCAGCTTAGAAAATCAATGCAAGCACAGATACAAAATTTATGTAGAAGGATGGGCATGGTCTGTGAGTGAAAAGTACATATTGGCATGTGATTCTATGACCTTATATGTAAACCCCCGTTACTACGATTTCTACATGAGAGGCATGCTACCATTGGAGCACTATTGGCCCATTAGGGACAACAGCAAGTGTACTTCTCTCAAATTTGCTGTGGAATGGGGCAATAATTACACGAAAAAGGCAGAGGCAATTGGGGAGGCAGCAAGCAACTTCATACAAGAAGATCTGAAGATGGACTATGTGTATGACTACATGTTTCATGTACTGAATGAATATGCAAAGCTCTTGAAATTCAAACCGACGATACATCCCAATGCATCGGAACTATGTTCTGAAACAATGGCATGCCCTGCAACTGGCACATGGAAGAAATTCATGGAGGAGTCCATGGTGATGTCTCCTAGTGATGAGATCCCATGTTCTTTGCCTCCTCCATATGATCCTCAAGCTCTTCGTGATTTTCTTGAGAGAAAAGCCAACTCAACTAGGCAAGTGGAAGCTTGGGAAAATGATTATTGGCAGAGTTCTAATAAAAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
Glyco_transf_90

Protein Analysis

525

Amino Acids

61.24

Weight (kDa)

8.52

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_90 PF05686 120 - 513 2.1e-194 Glycosyl transferase family 90
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 826
AccB7I CCANNNNNTGG 1 cut(s) 662
AccI GTMKAC 1 cut(s) 573
AccII CGCG 2 cut(s) 104, 451
AciI CCGC 2 cut(s) 102, 671
AclWI GGATC 5 cut(s) 79, 382, 395, 1438, 1466
AcoI YGGCCR 1 cut(s) 404
AcsI RAATTY 5 cut(s) 260, 1008, 1175, 1322, 1403
AcuI CTGAAG 1 cut(s) 1280
AfaI GTAC 4 cut(s) 965, 1049, 1165, 1296
AfiI CCNNNNNNNGG 5 cut(s) 662, 673, 859, 960, 1388
AflIII ACRYGT 2 cut(s) 534, 1284
AgsI TTSAA 3 cut(s) 260, 1321, 1327
AjnI CCWGG 3 cut(s) 498, 577, 869
AloI GAACNNNNNNTCC 2 cut(s) 701, 733
AluBI AGCT 6 cut(s) 562, 705, 978, 1315, 1481, 1532
AluI AGCT 6 cut(s) 562, 705, 978, 1315, 1481, 1532
Alw21I GWGCWC 1 cut(s) 1134
Alw26I GTCTC 4 cut(s) 410, 636, 743, 1434
AlwI GGATC 5 cut(s) 79, 382, 395, 1438, 1466
AoxI GGCC 5 cut(s) 404, 492, 623, 664, 1139
ApeKI GCWGC 5 cut(s) 82, 152, 559, 702, 1232
ApoI RAATTY 5 cut(s) 260, 1008, 1175, 1322, 1403
AseI ATTAAT 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 1403
AspLEI GCGC 2 cut(s) 451, 453
AspS9I GGNCC 2 cut(s) 665, 1140
AsuC2I CCSGG 2 cut(s) 169, 650
AsuHPI GGTGA 1 cut(s) 1435
BaeGI GKGCMC 1 cut(s) 831
BamHI GGATCC 1 cut(s) 387
BanI GGYRCC 1 cut(s) 826
Bbv12I GWGCWC 1 cut(s) 1134
BbvI GCAGC 5 cut(s) 94, 139, 571, 714, 1244
BccI CCATC 7 cut(s) 94, 361, 378, 379, 479, 1017, 1258
BceAI ACGGC 1 cut(s) 419
BciT130I CCWGG 3 cut(s) 500, 579, 871
BcnI CCSGG 2 cut(s) 169, 650
BcoDI GTCTC 4 cut(s) 410, 636, 743, 1434
BfaI CTAG 5 cut(s) 119, 321, 528, 1434, 1518
BglII AGATCT 2 cut(s) 397, 1255
BisI GCNGC 5 cut(s) 83, 153, 560, 703, 1233
BlsI GCNGC 5 cut(s) 84, 154, 561, 704, 1234
Bme1390I CCNGG 5 cut(s) 169, 500, 579, 650, 871
BmgT120I GGNCC 2 cut(s) 665, 1140
BmiI GGNNCC 4 cut(s) 389, 654, 828, 864
BmrFI CCNGG 5 cut(s) 169, 500, 579, 650, 871
BmsI GCATC 1 cut(s) 1358
BpmI CTGGAG 1 cut(s) 521
BpuEI CTTGAG 2 cut(s) 1461, 1517
BpuMI CCSGG 2 cut(s) 169, 650
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 4 cut(s) 407, 577, 764, 1419
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 5 cut(s) 662, 673, 859, 960, 1388
Bse118I RCCGGY 1 cut(s) 673
Bse1I ACTGG 3 cut(s) 845, 917, 1393
Bse3DI GCAATG 2 cut(s) 278, 898
BseBI CCWGG 3 cut(s) 500, 579, 871
BseDI CCNNGG 4 cut(s) 407, 577, 764, 1419
BseGI GGATG 3 cut(s) 490, 1028, 1339
BseLI CCNNNNNNNGG 5 cut(s) 662, 673, 859, 960, 1388
BseMI GCAATG 2 cut(s) 278, 898
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 3 cut(s) 845, 917, 1393
BsePI GCGCGC 1 cut(s) 449
BseRI GAGGAG 2 cut(s) 1427, 1452
BseSI GKGCMC 1 cut(s) 831
BseXI GCAGC 5 cut(s) 94, 139, 571, 714, 1244
BseYI CCCAGC 1 cut(s) 741
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 2 cut(s) 104, 451
BshFI GGCC 5 cut(s) 406, 494, 625, 666, 1141
BshNI GGYRCC 1 cut(s) 826
BsiHKAI GWGCWC 1 cut(s) 1134
BsiSI CCGG 3 cut(s) 168, 649, 674
BslFI GGGAC 3 cut(s) 446, 545, 1163
BslI CCNNNNNNNGG 5 cut(s) 662, 673, 859, 960, 1388
BsmAI GTCTC 4 cut(s) 410, 636, 743, 1434
BsmFI GGGAC 3 cut(s) 446, 545, 1163
BsnI GGCC 5 cut(s) 406, 494, 625, 666, 1141
Bso31I GGTCTC 1 cut(s) 743
Bsp1286I GDGCHC 2 cut(s) 831, 1134
Bsp143I GATC 9 cut(s) 71, 387, 397, 477, 600, 619, 1255, 1443, 1471
Bsp19I CCATGG 3 cut(s) 407, 764, 1419
BspACI CCGC 2 cut(s) 102, 671
BspANI GGCC 5 cut(s) 406, 494, 625, 666, 1141
BspCNI CTCAG 1 cut(s) 739
BspFNI CGCG 2 cut(s) 104, 451
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 4 cut(s) 389, 654, 828, 864
BspPI GGATC 5 cut(s) 79, 382, 395, 1438, 1466
BspQI GCTCTTC 1 cut(s) 1488
BspT107I GGYRCC 1 cut(s) 826
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 2 cut(s) 278, 898
BsrFI RCCGGY 1 cut(s) 673
BsrI ACTGG 3 cut(s) 845, 917, 1393
BssAI RCCGGY 1 cut(s) 673
BssECI CCNNGG 4 cut(s) 407, 577, 764, 1419
BssHII GCGCGC 1 cut(s) 449
BssMI GATC 9 cut(s) 71, 387, 397, 477, 600, 619, 1255, 1443, 1471
BssNAI GTATAC 1 cut(s) 574
BssT1I CCWWGG 3 cut(s) 407, 764, 1419
Bst1107I GTATAC 1 cut(s) 574
Bst2UI CCWGG 3 cut(s) 500, 579, 871
Bst4CI ACNGT 2 cut(s) 614, 689
Bst6I CTCTTC 2 cut(s) 89, 1488
BstAPI GCANNNNNTGC 1 cut(s) 1382
BstC8I GCNNGC 7 cut(s) 447, 451, 664, 995, 1118, 1237, 1378
BstDEI CTNAG 3 cut(s) 566, 747, 979
BstDSI CCRYGG 3 cut(s) 407, 764, 1419
BstEII GGTNACC 1 cut(s) 848
BstF5I GGATG 3 cut(s) 490, 1028, 1339
BstFNI CGCG 2 cut(s) 104, 451
BstHHI GCGC 2 cut(s) 451, 453
BstKTI GATC 9 cut(s) 74, 390, 400, 480, 603, 622, 1258, 1446, 1474
BstMAI GTCTC 4 cut(s) 410, 636, 743, 1434
BstMBI GATC 9 cut(s) 71, 387, 397, 477, 600, 619, 1255, 1443, 1471
BstMWI GCNNNNNNNGC 3 cut(s) 158, 1138, 1382
BstNI CCWGG 3 cut(s) 500, 579, 871
BstNSI RCATGY 6 cut(s) 369, 538, 1062, 1120, 1288, 1380
BstPI GGTNACC 1 cut(s) 848
BstSCI CCNGG 5 cut(s) 167, 498, 577, 648, 869
BstSLI GKGCMC 1 cut(s) 831
BstUI CGCG 2 cut(s) 104, 451
BstV1I GCAGC 5 cut(s) 94, 139, 571, 714, 1244
BstX2I RGATCY 4 cut(s) 387, 397, 1255, 1443
BstYI RGATCY 4 cut(s) 387, 397, 1255, 1443
BstZ17I GTATAC 1 cut(s) 574
BsuRI GGCC 5 cut(s) 406, 494, 625, 666, 1141
BtgI CCRYGG 3 cut(s) 407, 764, 1419
BtsCI GGATG 3 cut(s) 490, 1028, 1339
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 1 cut(s) 266
Cac8I GCNNGC 7 cut(s) 447, 451, 664, 995, 1118, 1237, 1378
CciI TCATGA 1 cut(s) 474
CfoI GCGC 2 cut(s) 451, 453
Cfr10I RCCGGY 1 cut(s) 673
Cfr13I GGNCC 2 cut(s) 665, 1140
Csp6I GTAC 4 cut(s) 964, 1048, 1164, 1295
CviQI GTAC 4 cut(s) 964, 1048, 1164, 1295
DdeI CTNAG 3 cut(s) 566, 747, 979
DpnI GATC 9 cut(s) 73, 389, 399, 479, 602, 621, 1257, 1445, 1473
DpnII GATC 9 cut(s) 71, 387, 397, 477, 600, 619, 1255, 1443, 1471
EaeI YGGCCR 1 cut(s) 404
Eam1104I CTCTTC 2 cut(s) 89, 1488
EarI CTCTTC 2 cut(s) 89, 1488
EciI GGCGGA 1 cut(s) 660
Eco130I CCWWGG 3 cut(s) 407, 764, 1419
Eco147I AGGCCT 1 cut(s) 494
Eco31I GGTCTC 1 cut(s) 743
Eco57I CTGAAG 1 cut(s) 1280
Eco91I GGTNACC 1 cut(s) 848
EcoO65I GGTNACC 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 3 cut(s) 498, 577, 869
EcoT14I CCWWGG 3 cut(s) 407, 764, 1419
EcoT22I ATGCAT 1 cut(s) 1351
ErhI CCWWGG 3 cut(s) 407, 764, 1419
FaqI GGGAC 3 cut(s) 446, 545, 1163
FauNDI CATATG 1 cut(s) 1468
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 5 cut(s) 83, 153, 560, 703, 1233
FokI GGATG 3 cut(s) 497, 1035, 1326
Fsp4HI GCNGC 5 cut(s) 83, 153, 560, 703, 1233
FspBI CTAG 5 cut(s) 119, 321, 528, 1434, 1518
GlaI GCGC 2 cut(s) 450, 452
GluI GCNGC 5 cut(s) 83, 153, 560, 703, 1233
GsaI CCCAGC 1 cut(s) 745
GsuI CTGGAG 1 cut(s) 521
HaeIII GGCC 5 cut(s) 406, 494, 625, 666, 1141
HapII CCGG 3 cut(s) 168, 649, 674
HhaI GCGC 2 cut(s) 451, 453
Hin6I GCGC 2 cut(s) 449, 451
HinP1I GCGC 2 cut(s) 449, 451
HindIII AAGCTT 1 cut(s) 1530
HinfI GANTC 7 cut(s) 188, 312, 423, 950, 971, 1064, 1415
HpaII CCGG 3 cut(s) 168, 649, 674
HphI GGTGA 1 cut(s) 1435
Hpy166II GTNNAC 4 cut(s) 540, 574, 1084, 1164
Hpy188I TCNGA 6 cut(s) 383, 683, 901, 1260, 1354, 1366
Hpy188III TCNNGA 5 cut(s) 475, 639, 1318, 1487, 1496
Hpy8I GTNNAC 4 cut(s) 540, 574, 1084, 1164
Hpy99I CGWCG 1 cut(s) 1336
HpyAV CCTTC 8 cut(s) 133, 359, 692, 788, 811, 890, 953, 1013
HpyCH4III ACNGT 2 cut(s) 614, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 1138, 1382
HpyF3I CTNAG 3 cut(s) 566, 747, 979
HspAI GCGC 2 cut(s) 449, 451
Kzo9I GATC 9 cut(s) 71, 387, 397, 477, 600, 619, 1255, 1443, 1471
LguI GCTCTTC 1 cut(s) 1488
LmnI GCTCC 3 cut(s) 442, 868, 1129
Lsp1109I GCAGC 5 cut(s) 94, 139, 571, 714, 1244
LweI GCATC 1 cut(s) 1358
MaeI CTAG 5 cut(s) 119, 321, 528, 1434, 1518
MaeIII GTNAC 4 cut(s) 292, 361, 848, 1091
MalI GATC 9 cut(s) 73, 389, 399, 479, 602, 621, 1257, 1445, 1473
MboI GATC 9 cut(s) 71, 387, 397, 477, 600, 619, 1255, 1443, 1471
MboII GAAGA 9 cut(s) 103, 106, 133, 204, 407, 1265, 1273, 1411, 1475
MfeI CAATTG 1 cut(s) 1221
MflI RGATCY 4 cut(s) 387, 397, 1255, 1443
MhlI GDGCHC 2 cut(s) 831, 1134
MluCI AATT 9 cut(s) 260, 285, 937, 1008, 1175, 1198, 1221, 1322, 1403
MlyI GAGTC 3 cut(s) 182, 980, 1424
MmeI TCCRAC 1 cut(s) 890
MnlI CCTC 8 cut(s) 678, 1106, 1210, 1222, 1405, 1470, 1473, 1485
Mph1103I ATGCAT 1 cut(s) 1351
MroXI GAANNNNTTC 1 cut(s) 1403
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 5 cut(s) 27, 271, 479, 1344, 1424
MspI CCGG 3 cut(s) 168, 649, 674
MspR9I CCNGG 5 cut(s) 169, 500, 579, 650, 871
MunI CAATTG 1 cut(s) 1221
MvaI CCWGG 3 cut(s) 500, 579, 871
MvnI CGCG 2 cut(s) 104, 451
MwoI GCNNNNNNNGC 3 cut(s) 158, 1138, 1382
NciI CCSGG 2 cut(s) 169, 650
NcoI CCATGG 3 cut(s) 407, 764, 1419
NdeI CATATG 1 cut(s) 1468
NdeII GATC 9 cut(s) 71, 387, 397, 477, 600, 619, 1255, 1443, 1471
NlaIV GGNNCC 4 cut(s) 389, 654, 828, 864
NmuCI GTSAC 2 cut(s) 292, 361
NsiI ATGCAT 1 cut(s) 1351
NspI RCATGY 6 cut(s) 369, 538, 1062, 1120, 1288, 1380
PaeI GCATGC 2 cut(s) 1120, 1380
PagI TCATGA 1 cut(s) 474
PauI GCGCGC 1 cut(s) 449
PceI AGGCCT 1 cut(s) 494
PciI ACATGT 2 cut(s) 534, 1284
PciSI GCTCTTC 1 cut(s) 1488
PdmI GAANNNNTTC 1 cut(s) 1403
PfeI GAWTC 4 cut(s) 312, 423, 950, 1064
PflMI CCANNNNNTGG 1 cut(s) 662
PfoI TCCNGGA 1 cut(s) 648
PkrI GCNGC 5 cut(s) 84, 154, 561, 704, 1234
PleI GAGTC 3 cut(s) 182, 979, 1423
PpsI GAGTC 3 cut(s) 182, 979, 1423
PscI ACATGT 2 cut(s) 534, 1284
PshBI ATTAAT 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 498, 577, 869
PspEI GGTNACC 1 cut(s) 848
PspFI CCCAGC 1 cut(s) 741
PspGI CCWGG 3 cut(s) 498, 577, 869
PspN4I GGNNCC 4 cut(s) 389, 654, 828, 864
PspPI GGNCC 2 cut(s) 665, 1140
PsuI RGATCY 4 cut(s) 387, 397, 1255, 1443
PteI GCGCGC 1 cut(s) 449
RsaI GTAC 4 cut(s) 965, 1049, 1165, 1296
RsaNI GTAC 4 cut(s) 964, 1048, 1164, 1295
RseI CAYNNNNRTG 5 cut(s) 27, 271, 479, 1344, 1424
SapI GCTCTTC 1 cut(s) 1488
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 5 cut(s) 83, 153, 560, 703, 1233
Sau3AI GATC 9 cut(s) 71, 387, 397, 477, 600, 619, 1255, 1443, 1471
Sau96I GGNCC 2 cut(s) 665, 1140
SchI GAGTC 3 cut(s) 182, 980, 1424
ScrFI CCNGG 5 cut(s) 169, 500, 579, 650, 871
SduI GDGCHC 2 cut(s) 831, 1134
SfaNI GCATC 1 cut(s) 1358
SmiMI CAYNNNNRTG 5 cut(s) 27, 271, 479, 1344, 1424
SmlI CTYRAG 2 cut(s) 1476, 1496
SmoI CTYRAG 2 cut(s) 1476, 1496
SphI GCATGC 2 cut(s) 1120, 1380
Sse9I AATT 9 cut(s) 260, 285, 937, 1008, 1175, 1198, 1221, 1322, 1403
SseBI AGGCCT 1 cut(s) 494
SsiI CCGC 2 cut(s) 102, 671
SspMI CTAG 5 cut(s) 119, 321, 528, 1434, 1518
StuI AGGCCT 1 cut(s) 494
StyD4I CCNGG 5 cut(s) 167, 498, 577, 648, 869
StyI CCWWGG 3 cut(s) 407, 764, 1419
TaaI ACNGT 2 cut(s) 614, 689
TaqI TCGA 1 cut(s) 89
TasI AATT 9 cut(s) 260, 285, 937, 1008, 1175, 1198, 1221, 1322, 1403
TatI WGTACW 3 cut(s) 1047, 1163, 1294
TfiI GAWTC 4 cut(s) 312, 423, 950, 1064
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 2 cut(s) 292, 361
TseI GCWGC 5 cut(s) 82, 152, 559, 702, 1232
Tsp45I GTSAC 2 cut(s) 292, 361
TspDTI ATGAA 6 cut(s) 17, 711, 1234, 1280, 1317, 1396
TspRI CASTG 1 cut(s) 273
Van91I CCANNNNNTGG 1 cut(s) 662
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 5 cut(s) 260, 1008, 1175, 1322, 1403
XceI RCATGY 6 cut(s) 369, 538, 1062, 1120, 1288, 1380
XmiI GTMKAC 1 cut(s) 573
XmnI GAANNNNTTC 1 cut(s) 1403
XspI CTAG 5 cut(s) 119, 321, 528, 1434, 1518
Zsp2I ATGCAT 1 cut(s) 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.