Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007594

Physical Location & Seq

Genomic Coordinates Forward (+)

16168146 .. 16172978

Gene Structure 4833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1056600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGGGGGGTCAATGCTTCAAATTCATACCATGTTGCTGGAATTCACAAATGAAGGCAGCGGTTCTCGAAACTCCTGACATTGATAATGAGGAGAAGAGCGAGGTTGATAACTTACCTGCATTTCGTGAATTCACATTTGAGCAACTTAAAAAGGCAACATCTGGTTTTGCCGTGGAGTATATTGTGTCTGAGCAAGGAGAGAAGGCTCCAAATATTGTTTATAAAGGGAAACTTGAAAATCAGAAGAGAATTGCTGTTAAGCGCTTCAATAGGATGGCCTGGCCCGATGCTCAGCAATTTTTGGAGGAAGCAAGATTGGTTGGTCACCTCCGCAACCATAGATTGGTCAATTTGCTTGGTTGTTGTTGCGAAGGTGATGAAAGGTTGCTTGTGGCGGAATATATGCCCAATGAAACACTTGCAAAGCATCTTTTTCATTGGGATGCACAGCCTATGAAATGGGTAATGCGATTAAGGGTTGTGTTACATCTTGCACAGGCTCTAGAATACTGCACAAGTAAAGGGCGTGCGCTTTATCACGACCTTAATGCTTACAGAATTTTATTCGATGAGGATGGCAATCCTAGAATATCCACCTTCGGACTGATGAAAAATAGTAGGGATGGGAAGAGTTATAGTACAAATCTGGCATTTACTCCACCTGAATATATTAGGAATGGGAGAGTAACACCAGAAAGTGCAATATATAGCTTTGGGACTCTCTTGATTGATCTTCTCAGCGGAAAGCATATCCCCCCTAGCCATGCCCTAGACCTGATTCGGGACAGAAATCTTCAGATGCTGACAGATTCTTGCTTGGAAGTACAGCTTTCTAATGATGATGGTACTGAGTTGGCACGCTTGGCTTCACGATGTTTACAATCTGAACCCCGAGAGCGACCAAATCCAAAGTCATTAGTTGCTTCTTTGACTCCTCTTCAGAAAGAAGATGAGGTTCCTTCTTCTGTTTTGATGGGTATCCCACACGGTACCTCCGGGTCTTCTCTTTCACCGCTTGGCGAAGCCTGCACAAGAAGGGATTTGACTGCTATACATGAGATCCTAGAGAGTATCGGCTATAAAGATGATGGAATGACAAATGAGCTCTCGTTCCAAATGTGGACTGACCAAATGCAGGAATCATTAGATTCAAAGAAAAAGGGGGATTCTGCTTTTAAGCACAAGGACTTCAGAACCACGATTGAGTGTTATTCACAGTTCATTGATGTTGGAACGATGGTTTCTCCAACAATTTTTGCCCGGCGTAGTTTGTCTTATCTCATGAGTGACAAGCCTCAGAATGCTATAAACGATGCAATGCAAGCACAAGTAGTTTCCCCAGTTTGGCACATTGCATGTTATCTTCAGGCTGTTGCCCTTTCAGCCCTTGGAATGGACAGTGAAGCTCAAGCAGCACTTAAGGAGGGTACTACGCTTGAATCCAAAAGGAGTAAAGCAGTTGGACAAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048545 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

491

Amino Acids

55.09

Weight (kDa)

6.11

Isoelectric Point (pI)

46.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	73 - 310	7e-30	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	73 - 308	3.7e-26	Protein kinase domain
TPR_BSK1_C	PF25575	341 - 457	2.3e-61	Serine/threonine-protein kinase BSK1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007594)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01740 AT1G01740 AT4G00710 AT4G00710
fragaria_vesca	FvH4_7g14010 FvH4_7g14010 FvH4_7g14010 FvH4_7g14010 FvH4_7g14010
malus_domestica	MD01G1056600.v1.1
prunus_persica	Prupe.2G167300_v2.0.a1
pyrus_communis	pycom01g08210 pycom01g08290 pycom01g08460
rosa_chinensis	RchiOBHm_Chr1g0354661
rosa_laevigata	RLG00000028189
rosa_multiflora	Rmu_sc0003940.1_g000008 Rmu_sc0006632.1_g000013
rosa_roxburghii	Rroxscaffold_4G00300860
rosa_rugosa	Rorug01G0243700
rosa_samantha	Rh1AG255000 Rh1AG255800 Rh1DG253600
rosa_wichuraiana	Rw1G022310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	222
Acc36I	ACCTGC	1 cut(s)	124
Acc65I	GGTACC	1 cut(s)	989
AccB1I	GGYRCC	1 cut(s)	989
AccB7I	CCANNNNNTGG	1 cut(s)	343
AciI	CCGC	5 cut(s)	59, 331, 395, 741, 1013
AclWI	GGATC	1 cut(s)	1054
AcsI	RAATTY	4 cut(s)	20, 40, 128, 558
AcuI	CTGAAG	4 cut(s)	779, 923, 1174, 1349
AfaI	GTAC	5 cut(s)	640, 825, 847, 991, 1429
AfeI	AGCGCT	1 cut(s)	263
AfiI	CCNNNNNNNGG	5 cut(s)	343, 781, 1135, 1344, 1393
AflII	CTTAAG	1 cut(s)	1418
AgsI	TTSAA	5 cut(s)	19, 236, 268, 1152, 1439
AjnI	CCWGG	1 cut(s)	278
AluBI	AGCT	4 cut(s)	711, 829, 1105, 1406
AluI	AGCT	4 cut(s)	711, 829, 1105, 1406
Alw21I	GWGCWC	1 cut(s)	1107
AlwI	GGATC	1 cut(s)	1054
AlwNI	CAGNNNCTG	1 cut(s)	802
Ama87I	CYCGRG	1 cut(s)	891
Aor51HI	AGCGCT	1 cut(s)	263
AoxI	GGCC	2 cut(s)	276, 281
ApeKI	GCWGC	2 cut(s)	56, 1412
ApoI	RAATTY	4 cut(s)	20, 40, 128, 558
ArsI	GACNNNNNNTTYG	2 cut(s)	896, 928
Asp718I	GGTACC	1 cut(s)	989
AspLEI	GCGC	2 cut(s)	264, 532
AspS9I	GGNCC	1 cut(s)	282
AsuC2I	CCSGG	2 cut(s)	997, 1261
AsuHPI	GGTGA	3 cut(s)	317, 386, 1002
AvaI	CYCGRG	1 cut(s)	891
BanI	GGYRCC	1 cut(s)	989
BanII	GRGCYC	1 cut(s)	1107
BbsI	GAAGAC	1 cut(s)	993
Bbv12I	GWGCWC	1 cut(s)	1107
BbvI	GCAGC	2 cut(s)	68, 1424
BccI	CCATC	7 cut(s)	268, 569, 617, 836, 967, 1082, 1231
BceAI	ACGGC	1 cut(s)	155
BcgI	CGANNNNNNTGC	2 cut(s)	530, 564
BciT130I	CCWGG	1 cut(s)	280
BciVI	GTATCC	1 cut(s)	989
BcnI	CCSGG	2 cut(s)	997, 1261
BfaI	CTAG	5 cut(s)	503, 585, 759, 770, 1064
BfoI	RGCGCY	1 cut(s)	265
BfrI	CTTAAG	1 cut(s)	1418
BfuAI	ACCTGC	1 cut(s)	124
BfuI	GTATCC	1 cut(s)	989
BisI	GCNGC	2 cut(s)	57, 1413
BlpI	GCTNAGC	1 cut(s)	291
BlsI	GCNGC	2 cut(s)	58, 1414
Bme1390I	CCNGG	3 cut(s)	280, 997, 1261
BmeT110I	CYCGRG	1 cut(s)	891
BmgT120I	GGNCC	1 cut(s)	282
BmiI	GGNNCC	3 cut(s)	207, 957, 991
BmrFI	CCNGG	3 cut(s)	280, 997, 1261
BmrI	ACTGGG	1 cut(s)	1334
BmsI	GCATC	5 cut(s)	277, 433, 436, 789, 1303
BmuI	ACTGGG	1 cut(s)	1334
BpiI	GAAGAC	1 cut(s)	993
Bpu1102I	GCTNAGC	1 cut(s)	291
BpuEI	CTTGAG	1 cut(s)	1392
BpuMI	CCSGG	2 cut(s)	997, 1261
BsaBI	GATNNNNATC	2 cut(s)	579, 977
BsaJI	CCNNGG	2 cut(s)	171, 1387
BsaXI	ACNNNNNCTCC	2 cut(s)	977, 1007
Bsc4I	CCNNNNNNNGG	5 cut(s)	343, 781, 1135, 1344, 1393
Bse1I	ACTGG	1 cut(s)	1340
Bse3DI	GCAATG	2 cut(s)	1323, 1350
Bse8I	GATNNNNATC	2 cut(s)	579, 977
BseBI	CCWGG	1 cut(s)	280
BseDI	CCNNGG	2 cut(s)	171, 1387
BseGI	GGATG	4 cut(s)	279, 448, 580, 628
BseJI	GATNNNNATC	2 cut(s)	579, 977
BseLI	CCNNNNNNNGG	5 cut(s)	343, 781, 1135, 1344, 1393
BseMI	GCAATG	2 cut(s)	1323, 1350
BseMII	CTCAG	5 cut(s)	180, 305, 751, 840, 1310
BseNI	ACTGG	1 cut(s)	1340
BseRI	GAGGAG	2 cut(s)	104, 924
BseXI	GCAGC	2 cut(s)	68, 1424
BsgI	GTGCAG	2 cut(s)	496, 1012
BshFI	GGCC	2 cut(s)	278, 283
BshNI	GGYRCC	1 cut(s)	989
BsiHKAI	GWGCWC	1 cut(s)	1107
BsiHKCI	CYCGRG	1 cut(s)	891
BsiSI	CCGG	2 cut(s)	996, 1261
BslFI	GGGAC	2 cut(s)	730, 797
BslI	CCNNNNNNNGG	5 cut(s)	343, 781, 1135, 1344, 1393
BsmFI	GGGAC	2 cut(s)	730, 797
BsmI	GAATGC	1 cut(s)	1306
BsnI	GGCC	2 cut(s)	278, 283
BsoBI	CYCGRG	1 cut(s)	891
Bsp1286I	GDGCHC	1 cut(s)	1107
Bsp143I	GATC	2 cut(s)	730, 1059
Bsp1720I	GCTNAGC	1 cut(s)	291
BspACI	CCGC	5 cut(s)	59, 331, 395, 741, 1013
BspANI	GGCC	2 cut(s)	278, 283
BspCNI	CTCAG	5 cut(s)	181, 304, 750, 841, 1309
BspHI	TCATGA	1 cut(s)	1281
BspLI	GGNNCC	3 cut(s)	207, 957, 991
BspMI	ACCTGC	1 cut(s)	124
BspPI	GGATC	1 cut(s)	1054
BspQI	GCTCTTC	1 cut(s)	89
BspT107I	GGYRCC	1 cut(s)	989
BspTI	CTTAAG	1 cut(s)	1418
BsrDI	GCAATG	2 cut(s)	1323, 1350
BsrI	ACTGG	1 cut(s)	1340
BssECI	CCNNGG	2 cut(s)	171, 1387
BssMI	GATC	2 cut(s)	730, 1059
BssT1I	CCWWGG	1 cut(s)	1387
Bst2UI	CCWGG	1 cut(s)	280
Bst4CI	ACNGT	3 cut(s)	989, 1218, 1400
Bst6I	CTCTTC	4 cut(s)	89, 239, 623, 942
BstAFI	CTTAAG	1 cut(s)	1418
BstC8I	GCNNGC	4 cut(s)	528, 859, 1027, 1323
BstDEI	CTNAG	5 cut(s)	189, 291, 737, 849, 1296
BstDSI	CCRYGG	1 cut(s)	171
BstEII	GGTNACC	1 cut(s)	323
BstF5I	GGATG	4 cut(s)	279, 448, 580, 628
BstH2I	RGCGCY	1 cut(s)	265
BstHHI	GCGC	2 cut(s)	264, 532
BstKTI	GATC	2 cut(s)	733, 1062
BstMBI	GATC	2 cut(s)	730, 1059
BstMWI	GCNNNNNNNGC	5 cut(s)	863, 1026, 1322, 1382, 1412
BstNI	CCWGG	1 cut(s)	280
BstNSI	RCATGY	1 cut(s)	1359
BstPI	GGTNACC	1 cut(s)	323
BstSCI	CCNGG	3 cut(s)	278, 995, 1259
BstV1I	GCAGC	2 cut(s)	68, 1424
BstV2I	GAAGAC	1 cut(s)	993
BstX2I	RGATCY	1 cut(s)	1059
BstXI	CCANNNNNNTGG	1 cut(s)	36
BstYI	RGATCY	1 cut(s)	1059
BsuI	GTATCC	1 cut(s)	989
BsuRI	GGCC	2 cut(s)	278, 283
BtgI	CCRYGG	1 cut(s)	171
BtsCI	GGATG	4 cut(s)	279, 448, 580, 628
BtsIMutI	CAGTG	1 cut(s)	1405
BveI	ACCTGC	1 cut(s)	124
Cac8I	GCNNGC	4 cut(s)	528, 859, 1027, 1323
CaiI	CAGNNNCTG	1 cut(s)	802
CciI	TCATGA	1 cut(s)	1281
CfoI	GCGC	2 cut(s)	264, 532
Cfr13I	GGNCC	1 cut(s)	282
Csp6I	GTAC	5 cut(s)	639, 824, 846, 990, 1428
CviAII	CATG	5 cut(s)	30, 764, 1055, 1282, 1356
CviQI	GTAC	5 cut(s)	639, 824, 846, 990, 1428
DdeI	CTNAG	5 cut(s)	189, 291, 737, 849, 1296
DpnI	GATC	2 cut(s)	732, 1061
DpnII	GATC	2 cut(s)	730, 1059
Eam1104I	CTCTTC	4 cut(s)	89, 239, 623, 942
EarI	CTCTTC	4 cut(s)	89, 239, 623, 942
EciI	GGCGGA	1 cut(s)	410
Ecl136II	GAGCTC	1 cut(s)	1105
Eco130I	CCWWGG	1 cut(s)	1387
Eco24I	GRGCYC	1 cut(s)	1107
Eco47III	AGCGCT	1 cut(s)	263
Eco53kI	GAGCTC	1 cut(s)	1105
Eco57I	CTGAAG	4 cut(s)	779, 923, 1174, 1349
Eco88I	CYCGRG	1 cut(s)	891
Eco91I	GGTNACC	1 cut(s)	323
EcoICRI	GAGCTC	1 cut(s)	1105
EcoO65I	GGTNACC	1 cut(s)	323
EcoRI	GAATTC	2 cut(s)	40, 128
EcoRII	CCWGG	1 cut(s)	278
EcoT14I	CCWWGG	1 cut(s)	1387
EcoT38I	GRGCYC	1 cut(s)	1107
ErhI	CCWWGG	1 cut(s)	1387
FaeI	CATG	5 cut(s)	33, 767, 1058, 1285, 1359
FalI	AAGNNNNNCTT	6 cut(s)	216, 248, 813, 845, 1401, 1433
FaqI	GGGAC	2 cut(s)	730, 797
FatI	CATG	5 cut(s)	29, 763, 1054, 1281, 1355
Fnu4HI	GCNGC	2 cut(s)	57, 1413
FokI	GGATG	4 cut(s)	286, 455, 587, 635
FriOI	GRGCYC	1 cut(s)	1107
Fsp4HI	GCNGC	2 cut(s)	57, 1413
FspBI	CTAG	5 cut(s)	503, 585, 759, 770, 1064
GlaI	GCGC	2 cut(s)	263, 531
GluI	GCNGC	2 cut(s)	57, 1413
HaeII	RGCGCY	1 cut(s)	265
HaeIII	GGCC	2 cut(s)	278, 283
HapII	CCGG	2 cut(s)	996, 1261
HhaI	GCGC	2 cut(s)	264, 532
Hin1II	CATG	5 cut(s)	33, 767, 1058, 1285, 1359
Hin6I	GCGC	2 cut(s)	262, 530
HinP1I	GCGC	2 cut(s)	262, 530
HinfI	GANTC	8 cut(s)	718, 778, 809, 931, 1139, 1148, 1166, 1439
HpaII	CCGG	2 cut(s)	996, 1261
HphI	GGTGA	3 cut(s)	317, 386, 1002
Hpy166II	GTNNAC	2 cut(s)	878, 1122
Hpy188I	TCNGA	8 cut(s)	190, 243, 602, 798, 886, 942, 1193, 1299
Hpy188III	TCNNGA	9 cut(s)	65, 74, 125, 503, 539, 724, 782, 870, 1282
Hpy8I	GTNNAC	2 cut(s)	878, 1122
HpyAV	CCTTC	6 cut(s)	46, 196, 365, 607, 969, 1029
HpyCH4III	ACNGT	3 cut(s)	989, 1218, 1400
HpyF10VI	GCNNNNNNNGC	5 cut(s)	863, 1026, 1322, 1382, 1412
HpyF3I	CTNAG	5 cut(s)	189, 291, 737, 849, 1296
Hsp92II	CATG	5 cut(s)	33, 767, 1058, 1285, 1359
HspAI	GCGC	2 cut(s)	262, 530
KpnI	GGTACC	1 cut(s)	993
Kzo9I	GATC	2 cut(s)	730, 1059
LguI	GCTCTTC	1 cut(s)	89
LmnI	GCTCC	1 cut(s)	211
Lsp1109I	GCAGC	2 cut(s)	68, 1424
LweI	GCATC	5 cut(s)	277, 433, 436, 789, 1303
MaeI	CTAG	5 cut(s)	503, 585, 759, 770, 1064
MaeIII	GTNAC	4 cut(s)	323, 483, 685, 1286
MalI	GATC	2 cut(s)	732, 1061
MboI	GATC	2 cut(s)	730, 1059
MflI	RGATCY	1 cut(s)	1059
MhlI	GDGCHC	1 cut(s)	1107
MluCI	AATT	8 cut(s)	20, 40, 128, 249, 296, 349, 558, 1251
MlyI	GAGTC	2 cut(s)	712, 925
MmeI	TCCRAC	3 cut(s)	1210, 1271, 1441
MseI	TTAA	6 cut(s)	147, 258, 473, 546, 1176, 1419
MslI	CAYNNNNRTG	1 cut(s)	441
MspA1I	CMGCKG	2 cut(s)	59, 741
MspCI	CTTAAG	1 cut(s)	1418
MspI	CCGG	2 cut(s)	996, 1261
MspR9I	CCNGG	3 cut(s)	280, 997, 1261
Mva1269I	GAATGC	1 cut(s)	1306
MvaI	CCWGG	1 cut(s)	280
MwoI	GCNNNNNNNGC	5 cut(s)	863, 1026, 1322, 1382, 1412
NciI	CCSGG	2 cut(s)	997, 1261
NdeII	GATC	2 cut(s)	730, 1059
NlaIII	CATG	5 cut(s)	33, 767, 1058, 1285, 1359
NlaIV	GGNNCC	3 cut(s)	207, 957, 991
NmuCI	GTSAC	2 cut(s)	323, 1286
NspI	RCATGY	1 cut(s)	1359
PagI	TCATGA	1 cut(s)	1281
PciSI	GCTCTTC	1 cut(s)	89
PctI	GAATGC	1 cut(s)	1306
PfeI	GAWTC	6 cut(s)	778, 809, 1139, 1148, 1166, 1439
PflMI	CCANNNNNTGG	1 cut(s)	343
PkrI	GCNGC	2 cut(s)	58, 1414
PleI	GAGTC	2 cut(s)	712, 925
PpsI	GAGTC	2 cut(s)	712, 925
PsiI	TTATAA	1 cut(s)	222
Psp124BI	GAGCTC	1 cut(s)	1107
Psp6I	CCWGG	1 cut(s)	278
PspEI	GGTNACC	1 cut(s)	323
PspGI	CCWGG	1 cut(s)	278
PspN4I	GGNNCC	3 cut(s)	207, 957, 991
PspPI	GGNCC	1 cut(s)	282
PstNI	CAGNNNCTG	1 cut(s)	802
PsuI	RGATCY	1 cut(s)	1059
RsaI	GTAC	5 cut(s)	640, 825, 847, 991, 1429
RsaNI	GTAC	5 cut(s)	639, 824, 846, 990, 1428
RseI	CAYNNNNRTG	1 cut(s)	441
SacI	GAGCTC	1 cut(s)	1107
SapI	GCTCTTC	1 cut(s)	89
SaqAI	TTAA	6 cut(s)	147, 258, 473, 546, 1176, 1419
SatI	GCNGC	2 cut(s)	57, 1413
Sau3AI	GATC	2 cut(s)	730, 1059
Sau96I	GGNCC	1 cut(s)	282
SchI	GAGTC	2 cut(s)	712, 925
ScrFI	CCNGG	3 cut(s)	280, 997, 1261
SduI	GDGCHC	1 cut(s)	1107
SfaNI	GCATC	5 cut(s)	277, 433, 436, 789, 1303
SmiMI	CAYNNNNRTG	1 cut(s)	441
SmlI	CTYRAG	2 cut(s)	1407, 1418
SmoI	CTYRAG	2 cut(s)	1407, 1418
Sse9I	AATT	8 cut(s)	20, 40, 128, 249, 296, 349, 558, 1251
SsiI	CCGC	5 cut(s)	59, 331, 395, 741, 1013
SspI	AATATT	1 cut(s)	214
SspMI	CTAG	5 cut(s)	503, 585, 759, 770, 1064
SstI	GAGCTC	1 cut(s)	1107
StyD4I	CCNGG	3 cut(s)	278, 995, 1259
StyI	CCWWGG	1 cut(s)	1387
TaaI	ACNGT	3 cut(s)	989, 1218, 1400
TaqI	TCGA	2 cut(s)	66, 567
TasI	AATT	8 cut(s)	20, 40, 128, 249, 296, 349, 558, 1251
TatI	WGTACW	2 cut(s)	638, 823
TfiI	GAWTC	6 cut(s)	778, 809, 1139, 1148, 1166, 1439
Tru1I	TTAA	6 cut(s)	147, 258, 473, 546, 1176, 1419
Tru9I	TTAA	6 cut(s)	147, 258, 473, 546, 1176, 1419
TscAI	CASTG	1 cut(s)	1405
TseFI	GTSAC	2 cut(s)	323, 1286
TseI	GCWGC	2 cut(s)	56, 1412
Tsp45I	GTSAC	2 cut(s)	323, 1286
TspDTI	ATGAA	8 cut(s)	13, 65, 393, 425, 426, 470, 623, 1210
TspRI	CASTG	1 cut(s)	1405
Van91I	CCANNNNNTGG	1 cut(s)	343
Vha464I	CTTAAG	1 cut(s)	1418
XapI	RAATTY	4 cut(s)	20, 40, 128, 558
XbaI	TCTAGA	1 cut(s)	502
XceI	RCATGY	1 cut(s)	1359
XspI	CTAG	5 cut(s)	503, 585, 759, 770, 1064

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.