MD01G1069700.v1.1

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007908
Physical Location & Seq
Forward (+)
17372294 .. 17373590
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1069700.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGGCGGCAACATGGATATAAGGTTTTGCATGGGCATGGGGACTGCTGCTTTCGGTGCTGTAAGATCGGATGAGAGTAATAGAAAGAGCACCTATGAAGTTTATGATCAGGAGGAGAGAAGAAGAAGTAGGATTTCATCAGATCCACCTCTTCAGCTTAATCTTTTGCCTTCTGCTCCAGTTCCTCGTAGCCACTTTTCTTCTCAGCTTCGCTTTCCATGGCTTACTAATAATCTGGATGTGTCTGATCCGGCCGGTTCATCAGATGGACCGGGAATAGGTTTGGATGTGAACCGTATGCCGGGAGTGTTGGCCTCGGCTAAGGAGGCCGAAGATGTGGCGGCTCAGCTGTCATCTTTGAATAGCACGGTTTCATCGTTTCAGATGGATTTTGGAATGAGAAATGGAGGAAGATTGAGCAAGAGAGATTTTGATGTTGACGTCGACAAAGCAAGTGATGACGAGGAGAACGGGTCAGCTCGGAAGAAACTCAGGCTCTCTAAAAGTCAATCGGCTTTTCTTGAAGAGAGCTTCAAAGAGCACAACACTCTTAATCCTAAGCAAAAACTTGTTTTGGCTAAACAGTTGAATCTTCGCCCACACCAAGTGGAAGTATGGTTTCAGAATCGAAAAGCAAGTTCTGCACTTAAAGAGAAATGGGCTACAACAAACTCCAGCCGTCGGACCCTGGAGGCTCTCCAGTCTCTACGTGGCGAAAACCCATCACTCCCACAAACTGCCACATCATCCTCATGTTCCAACAGCCGCAGCCCCCAGTCTCTACCTTTGAAGAAGAACTACCGATGCGTGCCATCTCTGCAGCAGTTCTACTCCGGCGGTCTCTTCGCTGTCTCATCCGACGGCTCATTCATCACCTGCAAATGCGATGAATCTAGTAAGATCGTAGATTCATCGAACGCCTCGATTAGGTCTACCATTGAAGGCGACTCAGATAAGATTACCGCTTTGGCCTTCAGCCCCGACAACCGGCTGTTCTTCTCCACCGGTCACAGTCGCCAAATTAAGATTGAGGACCTCTTCACCTTAAAGTGTGTGCGTTCTTGGAAGGGTCATGAGGGGCCAGTGATGGGAATGTCTTGTCACCCGTCTGGTGGATTGCTTGCGACTGCCGAAGCTGATAGGAAGGCCACAAAGGGGTTGTTTCTAGTGTTCTCTTCCATCCTGATCCTACTAAACAACTTCTTTTCTCTGCAAGCAATGATACAAATGTGCATGTTTGGGACCTAA

Protein Analysis

416

Amino Acids

45.63

Weight (kDa)

8.94

Isoelectric Point (pI)

58.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 161 - 212 7.1e-13 Homeodomain
Beta-prop_THOC3 PF25174 283 - 381 1.3e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 284 - 381 1.7e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 284 - 382 5.5e-08 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 286 - 381 2.3e-06 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 287 - 381 1.5e-10 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 290 - 382 2.2e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 884
AatII GACGTC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 884
AccI GTMKAC 2 cut(s) 444, 932
AciI CCGC 5 cut(s) 6, 341, 766, 837, 963
AclWI GGATC 3 cut(s) 137, 242, 1180
AcoI YGGCCR 1 cut(s) 252
AcuI CTGAAG 2 cut(s) 137, 958
AcyI GRCGYC 1 cut(s) 441
AdeI CACNNNGTG 1 cut(s) 607
AfiI CCNNNNNNNGG 8 cut(s) 278, 301, 681, 927, 987, 1088, 1112, 1155
AgeI ACCGGT 1 cut(s) 1004
AgsI TTSAA 6 cut(s) 361, 524, 535, 589, 790, 941
AjnI CCWGG 1 cut(s) 687
AluBI AGCT 6 cut(s) 157, 208, 349, 479, 531, 1136
AluI AGCT 6 cut(s) 157, 208, 349, 479, 531, 1136
Alw21I GWGCWC 2 cut(s) 92, 543
Alw26I GTCTC 4 cut(s) 708, 783, 845, 856
AlwI GGATC 3 cut(s) 137, 242, 1180
AoxI GGCC 6 cut(s) 252, 312, 327, 969, 1079, 1146
ApeKI GCWGC 3 cut(s) 47, 768, 820
AsiGI ACCGGT 1 cut(s) 1004
AspS9I GGNCC 5 cut(s) 269, 684, 1033, 1079, 1242
AsuC2I CCSGG 2 cut(s) 273, 303
AsuHPI GGTGA 3 cut(s) 865, 1033, 1094
AvaII GGWCC 4 cut(s) 269, 684, 1033, 1242
BaeI ACNNNNGTAYC 2 cut(s) 1214, 1247
BarI GAAGNNNNNNTAC 2 cut(s) 782, 814
Bbv12I GWGCWC 2 cut(s) 92, 543
BbvI GCAGC 3 cut(s) 34, 780, 832
BccI CCATC 6 cut(s) 260, 379, 730, 820, 1081, 1187
BceAI ACGGC 2 cut(s) 663, 877
BciT130I CCWGG 1 cut(s) 689
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 2 cut(s) 273, 303
BcoDI GTCTC 4 cut(s) 708, 783, 845, 856
BfaI CTAG 2 cut(s) 894, 1166
BfmI CTRYAG 1 cut(s) 818
BfuAI ACCTGC 1 cut(s) 884
BisI GCNGC 6 cut(s) 7, 48, 342, 766, 769, 821
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 6 cut(s) 8, 49, 343, 767, 770, 822
Bme1390I CCNGG 3 cut(s) 273, 303, 689
Bme18I GGWCC 4 cut(s) 269, 684, 1033, 1242
BmgT120I GGNCC 5 cut(s) 269, 684, 1033, 1079, 1242
BmiI GGNNCC 3 cut(s) 686, 1080, 1243
BmrFI CCNGG 3 cut(s) 273, 303, 689
BmrI ACTGGG 1 cut(s) 769
BmsI GCATC 1 cut(s) 794
BmuI ACTGGG 1 cut(s) 769
BpmI CTGGAG 4 cut(s) 162, 658, 683, 710
Bpu10I CCTNAGC 2 cut(s) 321, 558
Bpu1102I GCTNAGC 1 cut(s) 345
BpuMI CCSGG 2 cut(s) 273, 303
BsaAI YACGTR 1 cut(s) 710
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 845
BsaJI CCNNGG 3 cut(s) 218, 315, 687
BsaWI WCCGGW 1 cut(s) 1004
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 8 cut(s) 278, 301, 681, 927, 987, 1088, 1112, 1155
Bse118I RCCGGY 3 cut(s) 254, 987, 1004
Bse1I ACTGG 4 cut(s) 179, 700, 775, 1082
Bse3DI GCAATG 1 cut(s) 1224
BseBI CCWGG 1 cut(s) 689
BseDI CCNNGG 3 cut(s) 218, 315, 687
BseGI GGATG 6 cut(s) 76, 244, 292, 746, 854, 1179
BseLI CCNNNNNNNGG 8 cut(s) 278, 301, 681, 927, 987, 1088, 1112, 1155
BseMI GCAATG 1 cut(s) 1224
BseMII CTCAG 4 cut(s) 218, 359, 505, 963
BseNI ACTGG 4 cut(s) 179, 700, 775, 1082
BseRI GAGGAG 2 cut(s) 128, 479
BseX3I CGGCCG 1 cut(s) 252
BseXI GCAGC 3 cut(s) 34, 780, 832
BsgI GTGCAG 1 cut(s) 627
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 6 cut(s) 254, 314, 329, 971, 1081, 1148
BshTI ACCGGT 1 cut(s) 1004
BsiEI CGRYCG 1 cut(s) 255
BsiHKAI GWGCWC 2 cut(s) 92, 543
BsiSI CCGG 7 cut(s) 251, 255, 272, 302, 834, 988, 1005
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 8 cut(s) 278, 301, 681, 927, 987, 1088, 1112, 1155
BsmAI GTCTC 4 cut(s) 708, 783, 845, 856
BsmFI GGGAC 1 cut(s) 55
BsnI GGCC 6 cut(s) 254, 314, 329, 971, 1081, 1148
Bso31I GGTCTC 1 cut(s) 845
Bsp1286I GDGCHC 2 cut(s) 92, 543
Bsp143I GATC 6 cut(s) 65, 106, 142, 247, 900, 1185
Bsp1720I GCTNAGC 1 cut(s) 345
Bsp19I CCATGG 1 cut(s) 218
BspACI CCGC 5 cut(s) 6, 341, 766, 837, 963
BspANI GGCC 6 cut(s) 254, 314, 329, 971, 1081, 1148
BspCNI CTCAG 4 cut(s) 217, 358, 504, 962
BspHI TCATGA 1 cut(s) 1072
BspLI GGNNCC 3 cut(s) 686, 1080, 1243
BspMAI CTGCAG 1 cut(s) 822
BspMI ACCTGC 1 cut(s) 884
BspPI GGATC 3 cut(s) 137, 242, 1180
BspTNI GGTCTC 1 cut(s) 845
BsrDI GCAATG 1 cut(s) 1224
BsrFI RCCGGY 3 cut(s) 254, 987, 1004
BsrI ACTGG 4 cut(s) 179, 700, 775, 1082
BssAI RCCGGY 3 cut(s) 254, 987, 1004
BssECI CCNNGG 3 cut(s) 218, 315, 687
BssMI GATC 6 cut(s) 65, 106, 142, 247, 900, 1185
BssNI GRCGYC 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 4 cut(s) 296, 370, 585, 1013
Bst6I CTCTTC 5 cut(s) 156, 519, 848, 1043, 1180
BstACI GRCGYC 1 cut(s) 441
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 3 cut(s) 809, 1122, 1215
BstDEI CTNAG 6 cut(s) 204, 321, 345, 491, 558, 949
BstDSI CCRYGG 1 cut(s) 218
BstENI CCTNNNNNAGG 1 cut(s) 925
BstF5I GGATG 6 cut(s) 76, 244, 292, 746, 854, 1179
BstKTI GATC 6 cut(s) 68, 109, 145, 250, 903, 1188
BstMAI GTCTC 4 cut(s) 708, 783, 845, 856
BstMBI GATC 6 cut(s) 65, 106, 142, 247, 900, 1185
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 4 cut(s) 56, 326, 641, 817
BstNI CCWGG 1 cut(s) 689
BstNSI RCATGY 1 cut(s) 1237
BstSCI CCNGG 3 cut(s) 271, 301, 687
BstSFI CTRYAG 1 cut(s) 818
BstV1I GCAGC 3 cut(s) 34, 780, 832
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BstZI CGGCCG 1 cut(s) 252
BsuRI GGCC 6 cut(s) 254, 314, 329, 971, 1081, 1148
BtgI CCRYGG 1 cut(s) 218
BtgZI GCGATG 1 cut(s) 900
BtsCI GGATG 6 cut(s) 76, 244, 292, 746, 854, 1179
BtsIMutI CAGTG 1 cut(s) 1089
BveI ACCTGC 1 cut(s) 884
Cac8I GCNNGC 3 cut(s) 809, 1122, 1215
CciI TCATGA 1 cut(s) 1072
Cfr10I RCCGGY 3 cut(s) 254, 987, 1004
Cfr13I GGNCC 5 cut(s) 269, 684, 1033, 1079, 1242
CspAI ACCGGT 1 cut(s) 1004
CviAII CATG 7 cut(s) 13, 31, 37, 219, 753, 1073, 1234
DdeI CTNAG 6 cut(s) 204, 321, 345, 491, 558, 949
DpnI GATC 6 cut(s) 67, 108, 144, 249, 902, 1187
DpnII GATC 6 cut(s) 65, 106, 142, 247, 900, 1185
DraIII CACNNNGTG 1 cut(s) 607
EaeI YGGCCR 1 cut(s) 252
EagI CGGCCG 1 cut(s) 252
Eam1104I CTCTTC 5 cut(s) 156, 519, 848, 1043, 1180
EarI CTCTTC 5 cut(s) 156, 519, 848, 1043, 1180
EclXI CGGCCG 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 845
Eco47I GGWCC 4 cut(s) 269, 684, 1033, 1242
Eco52I CGGCCG 1 cut(s) 252
Eco57I CTGAAG 2 cut(s) 137, 958
EcoNI CCTNNNNNAGG 1 cut(s) 925
EcoO109I RGGNCCY 2 cut(s) 1033, 1242
EcoRII CCWGG 1 cut(s) 687
EcoT14I CCWWGG 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 218
FaeI CATG 7 cut(s) 16, 34, 40, 222, 756, 1076, 1237
FaqI GGGAC 1 cut(s) 55
FatI CATG 7 cut(s) 12, 30, 36, 218, 752, 1072, 1233
FbaI TGATCA 1 cut(s) 106
FblI GTMKAC 2 cut(s) 444, 932
Fnu4HI GCNGC 6 cut(s) 7, 48, 342, 766, 769, 821
FokI GGATG 6 cut(s) 83, 251, 299, 733, 841, 1166
Fsp4HI GCNGC 6 cut(s) 7, 48, 342, 766, 769, 821
FspBI CTAG 2 cut(s) 894, 1166
GluI GCNGC 6 cut(s) 7, 48, 342, 766, 769, 821
GsuI CTGGAG 4 cut(s) 162, 658, 683, 710
HaeIII GGCC 6 cut(s) 254, 314, 329, 971, 1081, 1148
HapII CCGG 7 cut(s) 251, 255, 272, 302, 834, 988, 1005
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 7 cut(s) 16, 34, 40, 222, 756, 1076, 1237
HincII GTYRAC 2 cut(s) 439, 445
HindII GTYRAC 2 cut(s) 439, 445
HinfI GANTC 5 cut(s) 589, 625, 890, 908, 947
HpaII CCGG 7 cut(s) 251, 255, 272, 302, 834, 988, 1005
HphI GGTGA 3 cut(s) 865, 1033, 1094
Hpy166II GTNNAC 4 cut(s) 292, 439, 445, 933
Hpy188III TCNNGA 5 cut(s) 110, 236, 521, 1073, 1183
Hpy8I GTNNAC 4 cut(s) 292, 439, 445, 933
Hpy99I CGWCG 3 cut(s) 446, 684, 863
HpyAV CCTTC 5 cut(s) 180, 935, 982, 1060, 1138
HpyCH4III ACNGT 4 cut(s) 296, 370, 585, 1013
HpyCH4IV ACGT 2 cut(s) 441, 709
HpyCH4V TGCA 6 cut(s) 30, 644, 820, 879, 1213, 1233
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 326, 641, 817
HpyF3I CTNAG 6 cut(s) 204, 321, 345, 491, 558, 949
HpySE526I ACGT 2 cut(s) 441, 709
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 7 cut(s) 16, 34, 40, 222, 756, 1076, 1237
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 6 cut(s) 65, 106, 142, 247, 900, 1185
LmnI GCTCC 1 cut(s) 181
Lsp1109I GCAGC 3 cut(s) 34, 780, 832
LweI GCATC 1 cut(s) 794
MaeI CTAG 2 cut(s) 894, 1166
MaeII ACGT 2 cut(s) 441, 709
MaeIII GTNAC 2 cut(s) 1007, 1100
MalI GATC 6 cut(s) 67, 108, 144, 249, 902, 1187
MboI GATC 6 cut(s) 65, 106, 142, 247, 900, 1185
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 2 cut(s) 92, 543
MluCI AATT 1 cut(s) 1020
MlyI GAGTC 1 cut(s) 941
MmeI TCCRAC 3 cut(s) 662, 783, 882
MseI TTAA 5 cut(s) 159, 552, 648, 1023, 1046
MslI CAYNNNNRTG 2 cut(s) 35, 751
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 7 cut(s) 251, 255, 272, 302, 834, 988, 1005
MspR9I CCNGG 3 cut(s) 273, 303, 689
MvaI CCWGG 1 cut(s) 689
MwoI GCNNNNNNNGC 4 cut(s) 56, 326, 641, 817
NciI CCSGG 2 cut(s) 273, 303
NcoI CCATGG 1 cut(s) 218
NdeII GATC 6 cut(s) 65, 106, 142, 247, 900, 1185
NlaIII CATG 7 cut(s) 16, 34, 40, 222, 756, 1076, 1237
NlaIV GGNNCC 3 cut(s) 686, 1080, 1243
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 2 cut(s) 1007, 1100
NspI RCATGY 1 cut(s) 1237
PagI TCATGA 1 cut(s) 1072
PaqCI CACCTGC 1 cut(s) 884
PcsI WCGNNNNNNNCGW 2 cut(s) 374, 920
PfeI GAWTC 4 cut(s) 589, 625, 890, 908
PinAI ACCGGT 1 cut(s) 1004
PkrI GCNGC 6 cut(s) 8, 49, 343, 767, 770, 822
PleI GAGTC 1 cut(s) 941
PpsI GAGTC 1 cut(s) 941
Ppu21I YACGTR 1 cut(s) 710
PpuMI RGGWCCY 2 cut(s) 1033, 1242
Psp5II RGGWCCY 2 cut(s) 1033, 1242
Psp6I CCWGG 1 cut(s) 687
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 3 cut(s) 686, 1080, 1243
PspPI GGNCC 5 cut(s) 269, 684, 1033, 1079, 1242
PspPPI RGGWCCY 2 cut(s) 1033, 1242
PstI CTGCAG 1 cut(s) 822
PsuI RGATCY 1 cut(s) 142
PvuII CAGCTG 1 cut(s) 349
RseI CAYNNNNRTG 2 cut(s) 35, 751
SalI GTCGAC 1 cut(s) 443
SaqAI TTAA 5 cut(s) 159, 552, 648, 1023, 1046
SatI GCNGC 6 cut(s) 7, 48, 342, 766, 769, 821
Sau3AI GATC 6 cut(s) 65, 106, 142, 247, 900, 1185
Sau96I GGNCC 5 cut(s) 269, 684, 1033, 1079, 1242
SchI GAGTC 1 cut(s) 941
ScrFI CCNGG 3 cut(s) 273, 303, 689
SduI GDGCHC 2 cut(s) 92, 543
SfaNI GCATC 1 cut(s) 794
SfcI CTRYAG 1 cut(s) 818
SinI GGWCC 4 cut(s) 269, 684, 1033, 1242
SmiMI CAYNNNNRTG 2 cut(s) 35, 751
Sse9I AATT 1 cut(s) 1020
SsiI CCGC 5 cut(s) 6, 341, 766, 837, 963
SspMI CTAG 2 cut(s) 894, 1166
StyD4I CCNGG 3 cut(s) 271, 301, 687
StyI CCWWGG 1 cut(s) 218
TaaI ACNGT 4 cut(s) 296, 370, 585, 1013
TaiI ACGT 2 cut(s) 444, 712
TaqI TCGA 4 cut(s) 444, 628, 914, 923
TasI AATT 1 cut(s) 1020
TauI GCSGC 3 cut(s) 9, 344, 768
TfiI GAWTC 4 cut(s) 589, 625, 890, 908
Tru1I TTAA 5 cut(s) 159, 552, 648, 1023, 1046
Tru9I TTAA 5 cut(s) 159, 552, 648, 1023, 1046
TscAI CASTG 1 cut(s) 1089
TseFI GTSAC 2 cut(s) 1007, 1100
TseI GCWGC 3 cut(s) 47, 768, 820
Tsp45I GTSAC 2 cut(s) 1007, 1100
TspDTI ATGAA 7 cut(s) 111, 126, 249, 363, 859, 900, 903
TspRI CASTG 1 cut(s) 1089
VpaK11BI GGWCC 4 cut(s) 269, 684, 1033, 1242
XagI CCTNNNNNAGG 1 cut(s) 925
XceI RCATGY 1 cut(s) 1237
XcmI CCANNNNNNNNNTGG 1 cut(s) 707
XmiI GTMKAC 2 cut(s) 444, 932
XspI CTAG 2 cut(s) 894, 1166
ZraI GACGTC 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.