pycom14g04600

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007908
Physical Location & Seq
Forward (+)
4280443 .. 4281721
1279 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04600.1

Sequence Viewer

Length: 624 bp
ATGTGTGCTTTATTTTGGTCCGAGCCGTCCGACCCCTCTCTTTTCTCTAGTCTCGTGCATGAACCGTTGACTTGTTCGCTTTGGCTTCTTTTTAAACTCCTCTCATCGCTTCCCTTCCCACCAAAAATTATTCAACAGCAAAAAATGGAGTTAGCTCTGAGCTTGGGGGATGCTTCAAAGCCGTTTCTGTTCCTTGACAAAGCTCCAAAGATTGGTAGCAACAAGGATCTAGGGTTTTGCATGGGCTTGGGGAGTGCTGCAAGATCGGATGAGAGTAGCAGAGAGAGCACCCATGAAGTTTACGATCAAGAGGAAGGAAGAAGAAGAAGTAGGGTTTCATCAGATCCACCTCTTCAGCTTAATCTCTTACCTTCTGCTCCAGTTCCTCGTAGCCATGCTTCTTCTCAGCTTCGCTTCCCATGGCTTACTGATAATCTTGCTGTGTCTGAACCGGCCGGTTCATCAGATGGACCGGGAATAGGGTTCGATGTGAACCGGATGCCTGGAGTGGTGGCCTCGGCTGAGGAGGCAGAAGATACGGCGGCGCCGCTATCATCTCCGAACAGCACAGTTTCATCGTTTCAGATGGATTTTGGAATAAGAAGTGGAGGAAGATCGAGTTAA

Protein Analysis

208

Amino Acids

22.3

Weight (kDa)

5.08

Isoelectric Point (pI)

76.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD-ZIP_N PF04618 75 - 194 9.5e-07 HD-ZIP protein N terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 544
AccB7I CCANNNNNTGG 1 cut(s) 212
AciI CCGC 2 cut(s) 542, 548
AclWI GGATC 2 cut(s) 234, 338
AcoI YGGCCR 1 cut(s) 453
AcuI CTGAAG 1 cut(s) 338
AcyI GRCGYC 1 cut(s) 545
AfiI CCNNNNNNNGG 2 cut(s) 212, 479
AgsI TTSAA 2 cut(s) 134, 177
AjnI CCWGG 1 cut(s) 502
AluBI AGCT 5 cut(s) 155, 162, 203, 358, 409
AluI AGCT 5 cut(s) 155, 162, 203, 358, 409
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 2 cut(s) 234, 338
AoxI GGCC 2 cut(s) 453, 513
ApeKI GCWGC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 2 cut(s) 18, 470
AsuC2I CCSGG 1 cut(s) 474
AvaII GGWCC 2 cut(s) 18, 470
BanI GGYRCC 1 cut(s) 544
BarI GAAGNNNNNNTAC 2 cut(s) 382, 414
BauI CACGAG 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 290
BbvCI CCTCAGC 1 cut(s) 522
BbvI GCAGC 1 cut(s) 244
BccI CCATC 2 cut(s) 461, 580
BceAI ACGGC 3 cut(s) 10, 166, 555
BciT130I CCWGG 1 cut(s) 504
BcnI CCSGG 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 2 cut(s) 48, 230
BfoI RGCGCY 1 cut(s) 548
BisI GCNGC 3 cut(s) 258, 543, 548
BlsI GCNGC 3 cut(s) 259, 544, 549
Bme1390I CCNGG 2 cut(s) 474, 504
Bme18I GGWCC 2 cut(s) 18, 470
BmgT120I GGNCC 2 cut(s) 18, 470
BmiI GGNNCC 1 cut(s) 546
BmrFI CCNGG 2 cut(s) 474, 504
BmsI GCATC 2 cut(s) 160, 489
BpmI CTGGAG 2 cut(s) 363, 525
Bpu10I CCTNAGC 1 cut(s) 522
BpuMI CCSGG 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 545
BsaJI CCNNGG 2 cut(s) 419, 516
BsaWI WCCGGW 1 cut(s) 495
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 479
Bse118I RCCGGY 2 cut(s) 451, 455
Bse1I ACTGG 1 cut(s) 380
BseBI CCWGG 1 cut(s) 504
BseDI CCNNGG 2 cut(s) 419, 516
BseGI GGATG 3 cut(s) 175, 274, 504
BseLI CCNNNNNNNGG 2 cut(s) 212, 479
BseMII CTCAG 3 cut(s) 149, 419, 513
BseNI ACTGG 1 cut(s) 380
BseRI GAGGAG 2 cut(s) 89, 539
BseX3I CGGCCG 1 cut(s) 453
BseXI GCAGC 1 cut(s) 244
Bsh1285I CGRYCG 1 cut(s) 456
BshFI GGCC 2 cut(s) 455, 515
BshNI GGYRCC 1 cut(s) 544
BsiEI CGRYCG 1 cut(s) 456
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 4 cut(s) 452, 456, 473, 496
BslI CCNNNNNNNGG 2 cut(s) 212, 479
BsmAI GTCTC 1 cut(s) 56
BsnI GGCC 2 cut(s) 455, 515
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 5 cut(s) 226, 263, 304, 343, 614
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 2 cut(s) 542, 548
BspANI GGCC 2 cut(s) 455, 515
BspCNI CTCAG 3 cut(s) 150, 418, 514
BspLI GGNNCC 1 cut(s) 546
BspPI GGATC 2 cut(s) 234, 338
BspT107I GGYRCC 1 cut(s) 544
BsrFI RCCGGY 2 cut(s) 451, 455
BsrI ACTGG 1 cut(s) 380
BssAI RCCGGY 2 cut(s) 451, 455
BssECI CCNNGG 2 cut(s) 419, 516
BssMI GATC 5 cut(s) 226, 263, 304, 343, 614
BssNI GRCGYC 1 cut(s) 545
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 2 cut(s) 66, 571
Bst6I CTCTTC 1 cut(s) 357
BstACI GRCGYC 1 cut(s) 545
BstDEI CTNAG 3 cut(s) 158, 405, 522
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 3 cut(s) 175, 274, 504
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 5 cut(s) 229, 266, 307, 346, 617
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 5 cut(s) 226, 263, 304, 343, 614
BstMCI CGRYCG 1 cut(s) 456
BstMWI GCNNNNNNNGC 2 cut(s) 285, 527
BstNI CCWGG 1 cut(s) 504
BstSCI CCNGG 2 cut(s) 472, 502
BstV1I GCAGC 1 cut(s) 244
BstX2I RGATCY 2 cut(s) 226, 343
BstYI RGATCY 2 cut(s) 226, 343
BstZI CGGCCG 1 cut(s) 453
BsuRI GGCC 2 cut(s) 455, 515
BtgI CCRYGG 1 cut(s) 419
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 3 cut(s) 175, 274, 504
CfoI GCGC 1 cut(s) 547
Cfr10I RCCGGY 2 cut(s) 451, 455
Cfr13I GGNCC 2 cut(s) 18, 470
CviAII CATG 5 cut(s) 59, 241, 293, 395, 420
DdeI CTNAG 3 cut(s) 158, 405, 522
DinI GGCGCC 1 cut(s) 546
DpnI GATC 5 cut(s) 228, 265, 306, 345, 616
DpnII GATC 5 cut(s) 226, 263, 304, 343, 614
DraI TTTAAA 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 453
EagI CGGCCG 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
EclXI CGGCCG 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 18, 470
Eco52I CGGCCG 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 419
EgeI GGCGCC 1 cut(s) 546
EheI GGCGCC 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 5 cut(s) 62, 244, 296, 398, 423
FaiI YATR 5 cut(s) 60, 242, 294, 396, 421
FatI CATG 5 cut(s) 58, 240, 292, 394, 419
Fnu4HI GCNGC 3 cut(s) 258, 543, 548
FokI GGATG 3 cut(s) 182, 281, 511
Fsp4HI GCNGC 3 cut(s) 258, 543, 548
FspBI CTAG 2 cut(s) 48, 230
GlaI GCGC 1 cut(s) 546
GluI GCNGC 3 cut(s) 258, 543, 548
GsuI CTGGAG 2 cut(s) 363, 525
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 2 cut(s) 455, 515
HapII CCGG 4 cut(s) 452, 456, 473, 496
HhaI GCGC 1 cut(s) 547
Hin1I GRCGYC 1 cut(s) 545
Hin1II CATG 5 cut(s) 62, 244, 296, 398, 423
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HpaII CCGG 4 cut(s) 452, 456, 473, 496
Hpy166II GTNNAC 3 cut(s) 69, 301, 493
Hpy188I TCNGA 9 cut(s) 22, 31, 159, 268, 343, 448, 466, 561, 585
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 3 cut(s) 69, 301, 493
HpyAV CCTTC 3 cut(s) 124, 308, 381
HpyCH4III ACNGT 2 cut(s) 66, 571
HpyCH4V TGCA 3 cut(s) 58, 240, 260
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 527
HpyF3I CTNAG 3 cut(s) 158, 405, 522
Hsp92I GRCGYC 1 cut(s) 545
Hsp92II CATG 5 cut(s) 62, 244, 296, 398, 423
HspAI GCGC 1 cut(s) 545
KasI GGCGCC 1 cut(s) 544
Kzo9I GATC 5 cut(s) 226, 263, 304, 343, 614
LmnI GCTCC 2 cut(s) 208, 382
LpnPI CCDG 7 cut(s) 393, 465, 469, 486, 489, 509, 516
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 2 cut(s) 160, 489
MaeI CTAG 2 cut(s) 48, 230
MalI GATC 5 cut(s) 228, 265, 306, 345, 616
MboI GATC 5 cut(s) 226, 263, 304, 343, 614
MboII GAAGA 7 cut(s) 330, 333, 336, 344, 393, 545, 624
MflI RGATCY 2 cut(s) 226, 343
MhlI GDGCHC 1 cut(s) 290
MluCI AATT 1 cut(s) 126
Mly113I GGCGCC 1 cut(s) 545
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 9 cut(s) 46, 110, 304, 360, 396, 517, 520, 526, 602
MseI TTAA 3 cut(s) 93, 360, 622
MspI CCGG 4 cut(s) 452, 456, 473, 496
MspR9I CCNGG 2 cut(s) 474, 504
MvaI CCWGG 1 cut(s) 504
MwoI GCNNNNNNNGC 2 cut(s) 285, 527
NarI GGCGCC 1 cut(s) 545
NciI CCSGG 1 cut(s) 474
NcoI CCATGG 1 cut(s) 419
NdeII GATC 5 cut(s) 226, 263, 304, 343, 614
NlaIII CATG 5 cut(s) 62, 244, 296, 398, 423
NlaIV GGNNCC 1 cut(s) 546
NmeAIII GCCGAG 1 cut(s) 497
PflMI CCANNNNNTGG 1 cut(s) 212
PkrI GCNGC 3 cut(s) 259, 544, 549
PluTI GGCGCC 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 502
PspGI CCWGG 1 cut(s) 502
PspN4I GGNNCC 1 cut(s) 546
PspPI GGNCC 2 cut(s) 18, 470
PsuI RGATCY 2 cut(s) 226, 343
SaqAI TTAA 3 cut(s) 93, 360, 622
SatI GCNGC 3 cut(s) 258, 543, 548
Sau3AI GATC 5 cut(s) 226, 263, 304, 343, 614
Sau96I GGNCC 2 cut(s) 18, 470
ScrFI CCNGG 2 cut(s) 474, 504
SduI GDGCHC 1 cut(s) 290
SetI ASST 7 cut(s) 157, 164, 205, 352, 360, 373, 411
SfaNI GCATC 2 cut(s) 160, 489
SfoI GGCGCC 1 cut(s) 546
SinI GGWCC 2 cut(s) 18, 470
Sse9I AATT 1 cut(s) 126
SsiI CCGC 2 cut(s) 542, 548
SspDI GGCGCC 1 cut(s) 544
SspMI CTAG 2 cut(s) 48, 230
StyD4I CCNGG 2 cut(s) 472, 502
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 2 cut(s) 66, 571
TaqI TCGA 2 cut(s) 486, 617
TasI AATT 1 cut(s) 126
TauI GCSGC 2 cut(s) 545, 550
Tru1I TTAA 3 cut(s) 93, 360, 622
Tru9I TTAA 3 cut(s) 93, 360, 622
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 5 cut(s) 75, 309, 327, 450, 564
Van91I CCANNNNNTGG 1 cut(s) 212
VpaK11BI GGWCC 2 cut(s) 18, 470
XspI CTAG 2 cut(s) 48, 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.