Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004897

Physical Location & Seq

Genomic Coordinates Forward (+)

18687282 .. 18691211

Gene Structure 3930 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1079600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2196 bp

ATGAATTCCAAGGGGCTTAAATTTGTAAGGTTTGAAGATTGGCAATCGGAGAGTTCTCTAAGTTCTGAAAGAGACGCTTCTGCTGATGATGGACGAAATCCAAGAAGAGCGATACCATCTGTGAATGCAGTTTTGAGGAGTATTCGGAGAGGACTAGATAGAGGTTCCGACAGGTTTATAAGCTCGAAAAAACCATTAAGTTCCACTTCCGTAGGCAACCGGCTAGCAAATGAGTCAGGCTCGAGGAGGAAAATTCTTGACCCTCAGGGGTCATTTCTTCAGAATTGGAATAAGATATTTGTGCTCTCTTGTGTCATTGCTGTGTCCTTGGACCCGCTGTTTCTTTATACCCCGGTGGTTGATGGCAAATACAAATGCGTTGATTTGGACAGGAGATTGCAGATCATTGCTTGTGTTCTTCGATCATTTACCGACATCTTTCATGTACTTCACATCATCTTTCAGTTCCGCACGGGGTTTATTGCCCCATCTTCTCGCGTATTTGGGAGGGGTGAGCTAATTGATGACCCCGTGGCCATTGCGAAAAGATACTTGTCCTCATACTTCATCATTGACATTCTAGCCATTCTTCCATTGCCACAGGCTGTAGTTTTTATTGTAATTCCTACAATATCACTTCCGGTTTCACTGGTCGCAAAGGACATGATGAAGTTTGTAATCGTTGCGCAATATGTGCCGAGATTTTGGAGGATCTATCCACTCTACAAAGAAGTAACAAGAACTTCTGGCTTCCTGACCGAAACAGCATGGGCCGGAGCTGCTTTCAATCTTCTGCTCTACATGCTAGCCAGTCATATAATTGGAGCCTTTTGGTACTTGTTCTCCGTGGAACGTGAGTATAGGTGCTGGCGCGATCAGTGCAATGGAGACCGTCGTTGTAAGAAGTTCCTGTACTGTAATTCTAAGAATGACACTGATGCAAGCTTTGTGGCAAATAAAAACGCTTCTTGCCCTTTCATTGACCCGGGCGACATTAAAAATTCATCGGTGTTCAACTTTGGGATATATTATGATGCTCTGGCGTCTGGTGTGGTGGAGACAAGAGATTTTCCAAAGAAATTGTCGTTCTGCTTCTGGTGGGGACTGCGCAACCTTAGTTCACTTGGCCAAAACCTCAAAACAAGTACATTTGTTTGGGAGATAGTCTTTGCAGTTACCATATCGATTTTGGGATTAGTTCTGTTTTCGTTGCTCATTGGCAATATGCAGAAATATCTGCAGTCCACAACTGTGAGAGTAGAAGAAATGAGAGTGAAAAGGCGCGATGCAGAACAGTGGATGGCACACCGAATGCTCCCGGAGAACTTGAGGGAAAGGATTAGACGGTATGATCAGTACAAATGGCAAGAAACAAGAGGTGTTGACGAAGATACTCTAGTTCGTAACCTTCCCAAGGACCTACGAAAGGACATAAAGCGCCATCTTTGCTTGGCTCTTCTCCAGAGAGTGCCAATTTTCGAAAAAATGGATGAACAGCTGATGGACGCCATGTGCGATCGCCTCAAGCCAGTACTATACACGGACAAGAGCTATATAACCCGAGAAGGTGATCCAGTAGATGAGATGCTGTTTATCATGCGCGGAAATTTAGCAACCATGACTACAAATGGTGGAAGAACTGGCTTCTTTAACACTGCTGATCTTAAGGCTGGTGACTTCTGTGGAGAAGAGCTTCTTACATGGGCCTTGGATCCAAACTCCTCCACCAATCTCCCCACCTCAACTAGGACAGTGGAAGCTAAAACCGAGGTTGAAGCGTTTGCGCTCATGGCTGATGATTTGAAGTTTGTCGCCTCCCAGTTTCGGCGCATGCACAGCAAGCAGCTCCAACACACTTTCAGGCAAGCTTCAGATTACAGTGATTCTAATGACTTGAAGGAAACTTGTTGGAGAACATGGTCAGCCTGTTTCATACAAGCAGCATGGCGCCGGCACTGGAAGAGAAAGCTCGAGAAGTCGTTACGCGAAGAAGAGGACAGATTGCAAGATGCTTTGACAAGAGATGCAGGAACCTCACCTAGCCTCCTTGCCGCTGTATATGCATCAAAGTTTGCAGCCAACGCGCTGCGAGGGTTGCGACAAAAGGGTAACCGCGACACTAGACCGCCACCAACATTGCTGCCTCTGCTGCCTCAGAAGCCCGCTGAGCCCGATTTTACTGCAGAACGTCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

732

Amino Acids

83.94

Weight (kDa)

9.32

Isoelectric Point (pI)

43.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	99 - 419	2.1e-13	Ion transport protein
cNMP_binding	PF00027	513 - 603	6.8e-06	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004897)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01340 AT1G01340 AT2G46430 AT2G46430 AT4G01010 AT4G01010
fragaria_vesca	FvH4_7g16300 FvH4_7g16300
malus_domestica	MD01G1079600.v1.1 MD07G1148400.v1.1
prunus_persica	Prupe.2G186700_v2.0.a1 Prupe.2G186700_v2.0.a1 Prupe.2G186700_v2.0.a1 Prupe.2G186900_v2.0.a1 Prupe.2G186900_v2.0.a1 Prupe.2G186900_v2.0.a1
pyrus_communis	pycom01g10790 pycom07g14740
rosa_chinensis	RchiOBHm_Chr1g0359311
rosa_laevigata	RLG00000027881
rosa_multiflora	Rmu_sc0005090.1_g000001 Rmu_sc0017222.1_g000004
rosa_roxburghii	Rroxscaffold_4G00297110
rosa_rugosa	Rorug01G0269500 Rorug01G0269600
rosa_samantha	Rh1AG283400 Rh1BG249600 Rh1CG267500 Rh1DG278500
rosa_wichuraiana	Rw1G025160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	179
Acc16I	TGCGCA	2 cut(s)	687, 1109
AccB1I	GGYRCC	1 cut(s)	1947
AccII	CGCG	7 cut(s)	498, 873, 1284, 1602, 1986, 2084, 2115
AciI	CCGC	7 cut(s)	335, 469, 1602, 2052, 2113, 2126, 2163
AclWI	GGATC	4 cut(s)	719, 1565, 1706, 1719
AcoI	YGGCCR	2 cut(s)	534, 1126
AcsI	RAATTY	5 cut(s)	4, 20, 252, 1000, 1606
AcuI	CTGAAG	2 cut(s)	263, 1854
AcyI	GRCGYC	3 cut(s)	1043, 1506, 1948
AfaI	GTAC	6 cut(s)	447, 836, 914, 1147, 1358, 1533
AfiI	CCNNNNNNNGG	4 cut(s)	1414, 1426, 1746, 1824
AflII	CTTAAG	1 cut(s)	1664
AgsI	TTSAA	6 cut(s)	35, 787, 1015, 1775, 1804, 1897
AleI	CACNNNNGTG	1 cut(s)	1250
Alw21I	GWGCWC	1 cut(s)	306
Alw26I	GTCTC	3 cut(s)	66, 882, 1052
AlwI	GGATC	4 cut(s)	719, 1565, 1706, 1719
Ama87I	CYCGRG	4 cut(s)	241, 985, 1560, 1970
AoxI	GGCC	4 cut(s)	534, 771, 1126, 1704
ApeKI	GCWGC	7 cut(s)	779, 1843, 1940, 2075, 2086, 2140, 2149
ApoI	RAATTY	5 cut(s)	4, 20, 252, 1000, 1606
ArsI	GACNNNNNNTTYG	2 cut(s)	1067, 1099
AsiSI	GCGATCGC	1 cut(s)	1519
Asp700I	GAANNNNTTC	1 cut(s)	1692
AspS9I	GGNCC	4 cut(s)	331, 771, 1417, 1704
AsuC2I	CCSGG	4 cut(s)	353, 986, 987, 1319
AsuHPI	GGTGA	4 cut(s)	524, 1580, 1685, 2028
AsuII	TTCGAA	1 cut(s)	1479
AsuNHI	GCTAGC	2 cut(s)	223, 805
AvaI	CYCGRG	4 cut(s)	241, 985, 1560, 1970
AvaII	GGWCC	2 cut(s)	331, 1417
AxyI	CCTNAGG	1 cut(s)	264
BaeI	ACNNNNGTAYC	2 cut(s)	1383, 1416
BalI	TGGCCA	2 cut(s)	536, 1128
BamHI	GGATCC	1 cut(s)	1711
BanI	GGYRCC	1 cut(s)	1947
BanII	GRGCYC	1 cut(s)	2172
BarI	GAAGNNNNNNTAC	2 cut(s)	896, 928
Bbv12I	GWGCWC	1 cut(s)	306
BbvI	GCAGC	7 cut(s)	766, 1855, 1952, 2073, 2087, 2127, 2136
BccI	CCATC	7 cut(s)	83, 124, 356, 496, 1294, 1449, 1495
BcgI	CGANNNNNNTGC	2 cut(s)	2162, 2196
BclI	TGATCA	1 cut(s)	1351
BcnI	CCSGG	4 cut(s)	353, 986, 987, 1319
BcoDI	GTCTC	3 cut(s)	66, 882, 1052
BfaI	CTAG	9 cut(s)	155, 224, 581, 806, 1397, 1746, 2040, 2121, 2194
BfmI	CTRYAG	3 cut(s)	606, 1238, 2181
BfoI	RGCGCY	2 cut(s)	1441, 1951
BfrI	CTTAAG	1 cut(s)	1664
BisI	GCNGC	8 cut(s)	780, 1844, 1941, 2052, 2076, 2087, 2141, 2150
BlpI	GCTNAGC	1 cut(s)	2166
BlsI	GCNGC	8 cut(s)	781, 1845, 1942, 2053, 2077, 2088, 2142, 2151
BmcAI	AGTACT	1 cut(s)	1533
Bme1390I	CCNGG	4 cut(s)	353, 986, 987, 1319
Bme18I	GGWCC	2 cut(s)	331, 1417
BmeT110I	CYCGRG	4 cut(s)	241, 985, 1560, 1970
BmgT120I	GGNCC	4 cut(s)	331, 771, 1417, 1704
BmiI	GGNNCC	6 cut(s)	166, 333, 826, 1713, 1949, 2032
BmrFI	CCNGG	4 cut(s)	353, 986, 987, 1319
BmrI	ACTGGG	1 cut(s)	1813
BmsI	GCATC	7 cut(s)	928, 1024, 1276, 1575, 1999, 2014, 2072
BmtI	GCTAGC	2 cut(s)	227, 809
BmuI	ACTGGG	1 cut(s)	1813
BplI	GAGNNNNNCTC	2 cut(s)	224, 256
BpmI	CTGGAG	1 cut(s)	1445
Bpu1102I	GCTNAGC	1 cut(s)	2166
Bpu14I	TTCGAA	1 cut(s)	1479
BpuEI	CTTGAG	2 cut(s)	1348, 1508
BpuMI	CCSGG	4 cut(s)	353, 986, 987, 1319
Bsa29I	ATCGAT	1 cut(s)	1184
BsaHI	GRCGYC	3 cut(s)	1043, 1506, 1948
BsaI	GGTCTC	1 cut(s)	882
BsaJI	CCNNGG	9 cut(s)	9, 327, 351, 531, 846, 985, 1413, 1707, 1767
BsaWI	WCCGGW	1 cut(s)	640
BsaXI	ACNNNNNCTCC	4 cut(s)	827, 857, 2028, 2058
Bsc4I	CCNNNNNNNGG	4 cut(s)	1414, 1426, 1746, 1824
Bse118I	RCCGGY	2 cut(s)	219, 1950
Bse1I	ACTGG	7 cut(s)	654, 810, 1529, 1574, 1645, 1819, 1961
Bse21I	CCTNAGG	1 cut(s)	264
Bse3DI	GCAATG	6 cut(s)	315, 405, 537, 593, 889, 2135
BseCI	ATCGAT	1 cut(s)	1184
BseDI	CCNNGG	9 cut(s)	9, 327, 351, 531, 846, 985, 1413, 1707, 1767
BseGI	GGATG	2 cut(s)	1305, 1495
BseLI	CCNNNNNNNGG	4 cut(s)	1414, 1426, 1746, 1824
BseMI	GCAATG	6 cut(s)	315, 405, 537, 593, 889, 2135
BseMII	CTCAG	3 cut(s)	278, 2157, 2168
BseNI	ACTGG	7 cut(s)	654, 810, 1529, 1574, 1645, 1819, 1961
BseRI	GAGGAG	3 cut(s)	151, 259, 1711
BseXI	GCAGC	7 cut(s)	766, 1855, 1952, 2073, 2087, 2127, 2136
Bsh1236I	CGCG	7 cut(s)	498, 873, 1284, 1602, 1986, 2084, 2115
Bsh1285I	CGRYCG	1 cut(s)	1519
BshFI	GGCC	4 cut(s)	536, 773, 1128, 1706
BshNI	GGYRCC	1 cut(s)	1947
BshVI	ATCGAT	1 cut(s)	1184
BsiEI	CGRYCG	1 cut(s)	1519
BsiHKAI	GWGCWC	1 cut(s)	306
BsiHKCI	CYCGRG	4 cut(s)	241, 985, 1560, 1970
BsiSI	CCGG	7 cut(s)	220, 353, 641, 774, 986, 1319, 1951
BslFI	GGGAC	1 cut(s)	1116
BslI	CCNNNNNNNGG	4 cut(s)	1414, 1426, 1746, 1824
BsmAI	GTCTC	3 cut(s)	66, 882, 1052
BsmBI	CGTCTC	1 cut(s)	66
BsmFI	GGGAC	1 cut(s)	1116
BsmI	GAATGC	2 cut(s)	130, 1317
BsnI	GGCC	4 cut(s)	536, 773, 1128, 1706
Bso31I	GGTCTC	1 cut(s)	882
BsoBI	CYCGRG	4 cut(s)	241, 985, 1560, 1970
Bsp119I	TTCGAA	1 cut(s)	1479
Bsp1286I	GDGCHC	2 cut(s)	306, 2172
Bsp143I	GATC	9 cut(s)	402, 422, 711, 874, 1351, 1516, 1570, 1660, 1711
Bsp1720I	GCTNAGC	1 cut(s)	2166
BspACI	CCGC	7 cut(s)	335, 469, 1602, 2052, 2113, 2126, 2163
BspANI	GGCC	4 cut(s)	536, 773, 1128, 1706
BspCNI	CTCAG	3 cut(s)	277, 2158, 2167
BspDI	ATCGAT	1 cut(s)	1184
BspFNI	CGCG	7 cut(s)	498, 873, 1284, 1602, 1986, 2084, 2115
BspLI	GGNNCC	6 cut(s)	166, 333, 826, 1713, 1949, 2032
BspMAI	CTGCAG	2 cut(s)	1242, 2185
BspOI	GCTAGC	2 cut(s)	227, 809
BspPI	GGATC	4 cut(s)	719, 1565, 1706, 1719
BspQI	GCTCTTC	3 cut(s)	100, 1461, 1683
BspT104I	TTCGAA	1 cut(s)	1479
BspT107I	GGYRCC	1 cut(s)	1947
BspTI	CTTAAG	1 cut(s)	1664
BspTNI	GGTCTC	1 cut(s)	882
BsrDI	GCAATG	6 cut(s)	315, 405, 537, 593, 889, 2135
BsrFI	RCCGGY	2 cut(s)	219, 1950
BsrI	ACTGG	7 cut(s)	654, 810, 1529, 1574, 1645, 1819, 1961
BssAI	RCCGGY	2 cut(s)	219, 1950
BssECI	CCNNGG	9 cut(s)	9, 327, 351, 531, 846, 985, 1413, 1707, 1767
BssMI	GATC	9 cut(s)	402, 422, 711, 874, 1351, 1516, 1570, 1660, 1711
BssNI	GRCGYC	3 cut(s)	1043, 1506, 1948
BssT1I	CCWWGG	4 cut(s)	9, 327, 1413, 1707
Bst4CI	ACNGT	7 cut(s)	893, 917, 1252, 1296, 1347, 1753, 1880
Bst6I	CTCTTC	5 cut(s)	100, 1461, 1683, 1955, 1986
BstACI	GRCGYC	3 cut(s)	1043, 1506, 1948
BstAFI	CTTAAG	1 cut(s)	1664
BstAPI	GCANNNNNTGC	1 cut(s)	694
BstBI	TTCGAA	1 cut(s)	1479
BstC8I	GCNNGC	9 cut(s)	225, 807, 869, 943, 1832, 1841, 1866, 1952, 2163
BstDEI	CTNAG	6 cut(s)	59, 264, 924, 1115, 2154, 2166
BstDSI	CCRYGG	2 cut(s)	531, 846
BstEII	GGTNACC	1 cut(s)	2108
BstENI	CCTNNNNNAGG	3 cut(s)	1412, 1424, 1744
BstF5I	GGATG	2 cut(s)	1305, 1495
BstFNI	CGCG	7 cut(s)	498, 873, 1284, 1602, 1986, 2084, 2115
BstH2I	RGCGCY	2 cut(s)	1441, 1951
BstKTI	GATC	9 cut(s)	405, 425, 714, 877, 1354, 1519, 1573, 1663, 1714
BstMAI	GTCTC	3 cut(s)	66, 882, 1052
BstMBI	GATC	9 cut(s)	402, 422, 711, 874, 1351, 1516, 1570, 1660, 1711
BstMCI	CGRYCG	1 cut(s)	1519
BstNSI	RCATGY	2 cut(s)	805, 1834
BstPI	GGTNACC	1 cut(s)	2108
BstSCI	CCNGG	4 cut(s)	351, 984, 985, 1317
BstSFI	CTRYAG	3 cut(s)	606, 1238, 2181
BstUI	CGCG	7 cut(s)	498, 873, 1284, 1602, 1986, 2084, 2115
BstV1I	GCAGC	7 cut(s)	766, 1855, 1952, 2073, 2087, 2127, 2136
BstX2I	RGATCY	2 cut(s)	711, 1711
BstYI	RGATCY	2 cut(s)	711, 1711
Bsu15I	ATCGAT	1 cut(s)	1184
Bsu36I	CCTNAGG	1 cut(s)	264
BsuRI	GGCC	4 cut(s)	536, 773, 1128, 1706
BsuTUI	ATCGAT	1 cut(s)	1184
BtgI	CCRYGG	2 cut(s)	531, 846
BtgZI	GCGATG	1 cut(s)	1299
BtsCI	GGATG	2 cut(s)	1305, 1495
BtsI	GCAGTG	1 cut(s)	1653
BtsIMutI	CAGTG	8 cut(s)	647, 884, 933, 1301, 1653, 1758, 1885, 1954
Cac8I	GCNNGC	9 cut(s)	225, 807, 869, 943, 1832, 1841, 1866, 1952, 2163
Cfr10I	RCCGGY	2 cut(s)	219, 1950
Cfr13I	GGNCC	4 cut(s)	331, 771, 1417, 1704
Cfr9I	CCCGGG	1 cut(s)	985
ClaI	ATCGAT	1 cut(s)	1184
CseI	GACGC	3 cut(s)	83, 1032, 1514
Csp6I	GTAC	6 cut(s)	446, 835, 913, 1146, 1357, 1532
CspCI	CAANNNNNGTGG	4 cut(s)	930, 965, 2118, 2153
CviQI	GTAC	6 cut(s)	446, 835, 913, 1146, 1357, 1532
DdeI	CTNAG	6 cut(s)	59, 264, 924, 1115, 2154, 2166
DinI	GGCGCC	1 cut(s)	1949
DpnI	GATC	9 cut(s)	404, 424, 713, 876, 1353, 1518, 1572, 1662, 1713
DpnII	GATC	9 cut(s)	402, 422, 711, 874, 1351, 1516, 1570, 1660, 1711
EaeI	YGGCCR	2 cut(s)	534, 1126
Eam1104I	CTCTTC	5 cut(s)	100, 1461, 1683, 1955, 1986
EarI	CTCTTC	5 cut(s)	100, 1461, 1683, 1955, 1986
Eco130I	CCWWGG	4 cut(s)	9, 327, 1413, 1707
Eco24I	GRGCYC	1 cut(s)	2172
Eco31I	GGTCTC	1 cut(s)	882
Eco47I	GGWCC	2 cut(s)	331, 1417
Eco57I	CTGAAG	2 cut(s)	263, 1854
Eco81I	CCTNAGG	1 cut(s)	264
Eco88I	CYCGRG	4 cut(s)	241, 985, 1560, 1970
Eco91I	GGTNACC	1 cut(s)	2108
EcoNI	CCTNNNNNAGG	3 cut(s)	1412, 1424, 1744
EcoO109I	RGGNCCY	1 cut(s)	1417
EcoO65I	GGTNACC	1 cut(s)	2108
EcoRI	GAATTC	1 cut(s)	4
EcoT14I	CCWWGG	4 cut(s)	9, 327, 1413, 1707
EcoT22I	ATGCAT	1 cut(s)	2065
EcoT38I	GRGCYC	1 cut(s)	2172
EgeI	GGCGCC	1 cut(s)	1949
EheI	GGCGCC	1 cut(s)	1949
ErhI	CCWWGG	4 cut(s)	9, 327, 1413, 1707
Esp3I	CGTCTC	1 cut(s)	66
FalI	AAGNNNNNCTT	6 cut(s)	61, 93, 190, 222, 1680, 1712
FaqI	GGGAC	1 cut(s)	1116
FauI	CCCGC	2 cut(s)	342, 2170
FbaI	TGATCA	1 cut(s)	1351
Fnu4HI	GCNGC	8 cut(s)	780, 1844, 1941, 2052, 2076, 2087, 2141, 2150
FokI	GGATG	2 cut(s)	1312, 1502
FriOI	GRGCYC	1 cut(s)	2172
Fsp4HI	GCNGC	8 cut(s)	780, 1844, 1941, 2052, 2076, 2087, 2141, 2150
FspBI	CTAG	9 cut(s)	155, 224, 581, 806, 1397, 1746, 2040, 2121, 2194
FspI	TGCGCA	2 cut(s)	687, 1109
GluI	GCNGC	8 cut(s)	780, 1844, 1941, 2052, 2076, 2087, 2141, 2150
GsuI	CTGGAG	1 cut(s)	1445
HaeII	RGCGCY	2 cut(s)	1441, 1951
HaeIII	GGCC	4 cut(s)	536, 773, 1128, 1706
HapII	CCGG	7 cut(s)	220, 353, 641, 774, 986, 1319, 1951
HgaI	GACGC	3 cut(s)	83, 1032, 1514
Hin1I	GRCGYC	3 cut(s)	1043, 1506, 1948
HincII	GTYRAC	1 cut(s)	1384
HindII	GTYRAC	1 cut(s)	1384
HindIII	AAGCTT	2 cut(s)	943, 1866
HinfI	GANTC	2 cut(s)	233, 1883
HpaII	CCGG	7 cut(s)	220, 353, 641, 774, 986, 1319, 1951
HphI	GGTGA	4 cut(s)	524, 1580, 1685, 2028
Hpy166II	GTNNAC	3 cut(s)	1121, 1245, 1384
Hpy188I	TCNGA	7 cut(s)	49, 67, 147, 169, 282, 1873, 2157
Hpy188III	TCNNGA	4 cut(s)	257, 754, 1462, 1972
Hpy8I	GTNNAC	3 cut(s)	1121, 1245, 1384
Hpy99I	CGWCG	1 cut(s)	897
HpyAV	CCTTC	3 cut(s)	1418, 1559, 1891
HpyCH4III	ACNGT	7 cut(s)	893, 917, 1252, 1296, 1347, 1753, 1880
HpyCH4IV	ACGT	2 cut(s)	853, 2188
HpyF3I	CTNAG	6 cut(s)	59, 264, 924, 1115, 2154, 2166
HpySE526I	ACGT	2 cut(s)	853, 2188
Hsp92I	GRCGYC	3 cut(s)	1043, 1506, 1948
KasI	GGCGCC	1 cut(s)	1947
KroI	GCCGGC	1 cut(s)	1950
KroNI	GCCGGC	1 cut(s)	1952
Ksp22I	TGATCA	1 cut(s)	1351
Kzo9I	GATC	9 cut(s)	402, 422, 711, 874, 1351, 1516, 1570, 1660, 1711
LguI	GCTCTTC	3 cut(s)	100, 1461, 1683
LmnI	GCTCC	4 cut(s)	776, 824, 1320, 1851
Lsp1109I	GCAGC	7 cut(s)	766, 1855, 1952, 2073, 2087, 2127, 2136
LweI	GCATC	7 cut(s)	928, 1024, 1276, 1575, 1999, 2014, 2072
MaeI	CTAG	9 cut(s)	155, 224, 581, 806, 1397, 1746, 2040, 2121, 2194
MaeII	ACGT	2 cut(s)	853, 2188
MaeIII	GTNAC	7 cut(s)	733, 1174, 1403, 1673, 1980, 2108, 2189
MalI	GATC	9 cut(s)	404, 424, 713, 876, 1353, 1518, 1572, 1662, 1713
MboI	GATC	9 cut(s)	402, 422, 711, 874, 1351, 1516, 1570, 1660, 1711
MflI	RGATCY	2 cut(s)	711, 1711
MhlI	GDGCHC	2 cut(s)	306, 2172
MlsI	TGGCCA	2 cut(s)	536, 1128
MluNI	TGGCCA	2 cut(s)	536, 1128
Mly113I	GGCGCC	1 cut(s)	1948
MlyI	GAGTC	1 cut(s)	242
MmeI	TCCRAC	3 cut(s)	192, 1873, 1889
Mox20I	TGGCCA	2 cut(s)	536, 1128
Mph1103I	ATGCAT	1 cut(s)	2065
MroNI	GCCGGC	1 cut(s)	1950
MroXI	GAANNNNTTC	1 cut(s)	1692
MscI	TGGCCA	2 cut(s)	536, 1128
MseI	TTAA	5 cut(s)	18, 197, 996, 1650, 1665
MslI	CAYNNNNRTG	2 cut(s)	320, 1250
Msp20I	TGGCCA	2 cut(s)	536, 1128
MspA1I	CMGCKG	4 cut(s)	337, 1498, 2054, 2165
MspCI	CTTAAG	1 cut(s)	1664
MspI	CCGG	7 cut(s)	220, 353, 641, 774, 986, 1319, 1951
MspR9I	CCNGG	4 cut(s)	353, 986, 987, 1319
Mva1269I	GAATGC	2 cut(s)	130, 1317
MvnI	CGCG	7 cut(s)	498, 873, 1284, 1602, 1986, 2084, 2115
NaeI	GCCGGC	1 cut(s)	1952
NarI	GGCGCC	1 cut(s)	1948
NciI	CCSGG	4 cut(s)	353, 986, 987, 1319
NdeII	GATC	9 cut(s)	402, 422, 711, 874, 1351, 1516, 1570, 1660, 1711
NgoMIV	GCCGGC	1 cut(s)	1950
NheI	GCTAGC	2 cut(s)	223, 805
NlaIV	GGNNCC	6 cut(s)	166, 333, 826, 1713, 1949, 2032
NmeAIII	GCCGAG	1 cut(s)	723
NmuCI	GTSAC	2 cut(s)	1673, 2189
NsbI	TGCGCA	2 cut(s)	687, 1109
NsiI	ATGCAT	1 cut(s)	2065
NspI	RCATGY	2 cut(s)	805, 1834
NspV	TTCGAA	1 cut(s)	1479
OliI	CACNNNNGTG	1 cut(s)	1250
PaeI	GCATGC	1 cut(s)	1834
PaeR7I	CTCGAG	2 cut(s)	241, 1970
PciSI	GCTCTTC	3 cut(s)	100, 1461, 1683
PcsI	WCGNNNNNNNCGW	1 cut(s)	1512
PctI	GAATGC	2 cut(s)	130, 1317
PdiI	GCCGGC	1 cut(s)	1952
PdmI	GAANNNNTTC	1 cut(s)	1692
PfeI	GAWTC	1 cut(s)	1883
PfoI	TCCNGGA	1 cut(s)	1317
PkrI	GCNGC	8 cut(s)	781, 1845, 1942, 2053, 2077, 2088, 2142, 2151
Ple19I	CGATCG	1 cut(s)	1519
PleI	GAGTC	1 cut(s)	241
PluTI	GGCGCC	1 cut(s)	1951
PpsI	GAGTC	1 cut(s)	241
PpuMI	RGGWCCY	1 cut(s)	1417
PsiI	TTATAA	1 cut(s)	179
Psp5II	RGGWCCY	1 cut(s)	1417
PspEI	GGTNACC	1 cut(s)	2108
PspN4I	GGNNCC	6 cut(s)	166, 333, 826, 1713, 1949, 2032
PspPI	GGNCC	4 cut(s)	331, 771, 1417, 1704
PspPPI	RGGWCCY	1 cut(s)	1417
PspXI	VCTCGAGB	1 cut(s)	241
PstI	CTGCAG	2 cut(s)	1242, 2185
PsuI	RGATCY	2 cut(s)	711, 1711
PvuI	CGATCG	1 cut(s)	1519
PvuII	CAGCTG	1 cut(s)	1498
RgaI	GCGATCGC	1 cut(s)	1519
RsaI	GTAC	6 cut(s)	447, 836, 914, 1147, 1358, 1533
RsaNI	GTAC	6 cut(s)	446, 835, 913, 1146, 1357, 1532
RseI	CAYNNNNRTG	2 cut(s)	320, 1250
SapI	GCTCTTC	3 cut(s)	100, 1461, 1683
SaqAI	TTAA	5 cut(s)	18, 197, 996, 1650, 1665
SatI	GCNGC	8 cut(s)	780, 1844, 1941, 2052, 2076, 2087, 2141, 2150
Sau3AI	GATC	9 cut(s)	402, 422, 711, 874, 1351, 1516, 1570, 1660, 1711
Sau96I	GGNCC	4 cut(s)	331, 771, 1417, 1704
ScaI	AGTACT	1 cut(s)	1533
SchI	GAGTC	1 cut(s)	242
ScrFI	CCNGG	4 cut(s)	353, 986, 987, 1319
SduI	GDGCHC	2 cut(s)	306, 2172
SfaAI	GCGATCGC	1 cut(s)	1519
SfaNI	GCATC	7 cut(s)	928, 1024, 1276, 1575, 1999, 2014, 2072
SfcI	CTRYAG	3 cut(s)	606, 1238, 2181
SfoI	GGCGCC	1 cut(s)	1949
Sfr274I	CTCGAG	2 cut(s)	241, 1970
SfuI	TTCGAA	1 cut(s)	1479
SgfI	GCGATCGC	1 cut(s)	1519
SinI	GGWCC	2 cut(s)	331, 1417
SlaI	CTCGAG	2 cut(s)	241, 1970
SmaI	CCCGGG	1 cut(s)	987
SmiMI	CAYNNNNRTG	2 cut(s)	320, 1250
SmlI	CTYRAG	5 cut(s)	241, 1327, 1523, 1664, 1970
SmoI	CTYRAG	5 cut(s)	241, 1327, 1523, 1664, 1970
SphI	GCATGC	1 cut(s)	1834
SsiI	CCGC	7 cut(s)	335, 469, 1602, 2052, 2113, 2126, 2163
SspDI	GGCGCC	1 cut(s)	1947
SspMI	CTAG	9 cut(s)	155, 224, 581, 806, 1397, 1746, 2040, 2121, 2194
StyD4I	CCNGG	4 cut(s)	351, 984, 985, 1317
StyI	CCWWGG	4 cut(s)	9, 327, 1413, 1707
TaaI	ACNGT	7 cut(s)	893, 917, 1252, 1296, 1347, 1753, 1880
TaiI	ACGT	2 cut(s)	856, 2191
TaqI	TCGA	6 cut(s)	185, 242, 421, 1184, 1479, 1971
TaqII	GACCGA	1 cut(s)	773
TatI	WGTACW	5 cut(s)	445, 912, 1145, 1356, 1531
TauI	GCSGC	1 cut(s)	2054
TfiI	GAWTC	1 cut(s)	1883
Tru1I	TTAA	5 cut(s)	18, 197, 996, 1650, 1665
Tru9I	TTAA	5 cut(s)	18, 197, 996, 1650, 1665
TscAI	CASTG	8 cut(s)	654, 884, 940, 1301, 1660, 1758, 1885, 1961
TseFI	GTSAC	2 cut(s)	1673, 2189
TseI	GCWGC	7 cut(s)	779, 1843, 1940, 2075, 2086, 2140, 2149
Tsp45I	GTSAC	2 cut(s)	1673, 2189
TspDTI	ATGAA	8 cut(s)	17, 431, 556, 683, 967, 993, 1506, 1921
TspGWI	ACGGA	3 cut(s)	199, 835, 1556
TspMI	CCCGGG	1 cut(s)	985
TspRI	CASTG	8 cut(s)	654, 884, 940, 1301, 1660, 1758, 1885, 1961
Vha464I	CTTAAG	1 cut(s)	1664
VpaK11BI	GGWCC	2 cut(s)	331, 1417
XagI	CCTNNNNNAGG	3 cut(s)	1412, 1424, 1744
XapI	RAATTY	5 cut(s)	4, 20, 252, 1000, 1606
XceI	RCATGY	2 cut(s)	805, 1834
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1186
XhoI	CTCGAG	2 cut(s)	241, 1970
XmaI	CCCGGG	1 cut(s)	985
XmnI	GAANNNNTTC	1 cut(s)	1692
XspI	CTAG	9 cut(s)	155, 224, 581, 806, 1397, 1746, 2040, 2121, 2194
ZrmI	AGTACT	1 cut(s)	1533
Zsp2I	ATGCAT	1 cut(s)	2065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.