MD07G1148400.v1.1

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004897
Physical Location & Seq
Forward (+)
21708909 .. 21711646
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1148400.v1.1.491

Sequence Viewer

Length: 2073 bp
ATGCAGTTTTGTGGAGTATTCGGAGCGGACTGGAGAGAGGTTCTGACAGGTCACCGGCTAACAAATGAGTCAGGCTCCAGGAGGAGAATTCTTCACCCTCAGGGGCCATTTCTTCAGAATTGGAATAGGATATTTGTGCTCTCTTGTGTCATAGCTGTGTCCTTGGACCCGCTGTTTTTTTATACCGCGGTGGTTGATGGCAAAAAGAAATGCCTCAATTTGCACAAGACATTGCAGATCATTGTTTGTGTTCTTCGATCATGTACCGACATCTTTCATGTATTTCACATCATCCTTCAGTTCCGCACGGGGTTTATTGCCCCATCTTCTCGCGTATTTGGGAGGGGTGAGCTAATTGATGATCCCGTGGCCATTGCGAAAAGATACTTGTCCTCATACTTCATCATTGACATTCTAGCCATTCTTCCCCTGCCACAGGTTGTAGTTTTTGTTATAATCCCTACAATCTCGGGTCCGGTTCCACTGGTTGCAAAGGACTTGTTGAAGTTTATAATCGTTGCGCAATATGTGCCGAGATTTTGGAGGATCTATCCACTCTACCAAGAAGTAACGAGAACTTCTGGTTTCATGACTGAAACAGCGTGGGCCGGGGCTGCTGTCAATCTTCTGCTCTTCATGCTTGCCAGTCATATAGTTGGAGCCTTTTGGTACTTGCTCTCCGTGGAACGTGAGTACAGGTGCTGGCGCGATCAGTGCAATGGGAACGTTGATTGTAAGAAATTCCTGTACTGTGTTCCTAACCATGACAATGATGCAAGCTTTGTTGCAAAGATGAACGCTTCTTGCCCTTTCAGTGACCCGGGCAACATTAAAAATTCATCAGCGTTCAACTTTGGAATATATAATGATGCTCTTGCGTCTGGTGTGACGGAGACCAAAGATTTTCCAAAGAAATTCTTGTTCTGCTTCTGGTGGGGTCTGCGCAACCTTAGTTCACTTGGCCAAAACCTCCAAACAAGCACATATGTTTGGGAGATAATATTTGCAGTCGCCATATCAATCTTCGGATTAGTTCTGTTTTCGTTGCTCATTGGAAATATGCAGAAGTATCTGCAGTCCACAACTGTGAGGGTTGAAGAAATGAGAGTGAAAAGGCGCGATGCAGAACAGTGGATGTCACACCGAATGCTCCCAGAGAAATTGAGGGAAAGGATTCGACGGTATGATCAGTACAAATGGCAAGAAACAAGAGGTGTTGAGGAAGACAATCTTGTTCGTAACCTTCCTAAAGACCTACGAAAGGACATAAAGCGCCATCTTTGCTTGGCTCTTCTCAAGAGAGTGCCAATGTTCGAAAAAATGGATGAACAACTGATGGATGCCATGTGCGATCGCCTCAAGCCAGTTCTGTATACGGACAAGAGCTTCATAACCCGAGAAGGTGATCCAGTAGATGAGATGCTCTTTATTATGCGCGGAAATTTAGCAACTATGACTACGAATGGTGGAAGAACAGGCTTCTTTAACACAGCTGATCTTAAGGCTGGTGACTTCTGTGGAGAAGAGCTTCTTACATGGGCCTTGGTTCCAAACTCCTCCACCAATCTCCCCACCTCAACTAGGACAGTGGAAGCTAAAACTGAGGTTGAAGCGTTTGCGCTCATGGCTGATGATTTGAAGTTTGTCGCCTCCCAGTTTCGGCGCCTGCACAGCAAGCAGCTCCGACATACTTTCAGGCAAGGCTCTAATTATAGTAATTCTGATGACTTGAAGGAAATTTGTGGTATGAAAAAGCAAAGAATTAAACCGATATATGGACTACGGTTCTACTCCCTGCAGTGGAGAACACGGTCAGCCTGTTTCATACAAGCAGCTTGGCGCCGGCACTGGAAGAGAAAGCTCGAAAAGTCCTTACGCGAAGAAGAAGACAGGCTGCAAGATGCAGGAACCTTACCAAGCCTCCTTGCCGCTGTATATGCATCAAAGTTTGCAGCCAACGCGCTGCGAGGGTTGCGACAAAAGGTTAAGCGCGACACTAGGCCTTCACCGACATTGCTGCCATTGCTGCATCAGAAGCCCGCTGAGCCCGATTTTACTGCAGAACATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

691

Amino Acids

79.57

Weight (kDa)

9.42

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 42 - 364 8e-17 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 455, 512
Acc16I TGCGCA 2 cut(s) 522, 944
AccB1I GGYRCC 2 cut(s) 1662, 1839
AccBSI CCGCTC 1 cut(s) 26
AccI GTMKAC 1 cut(s) 1373
AccII CGCG 8 cut(s) 188, 333, 708, 1119, 1437, 1878, 1961, 1992
AciI CCGC 8 cut(s) 26, 170, 186, 188, 304, 1437, 1929, 2040
AclI AACGTT 1 cut(s) 726
AclWI GGATC 3 cut(s) 356, 554, 1400
AcoI YGGCCR 2 cut(s) 369, 961
AcsI RAATTY 6 cut(s) 87, 740, 835, 914, 1441, 1737
AcuI CTGAAG 2 cut(s) 98, 281
AcyI GRCGYC 2 cut(s) 1663, 1840
AfaI GTAC 5 cut(s) 265, 671, 695, 749, 1193
AfiI CCNNNNNNNGG 6 cut(s) 436, 1261, 1581, 1659, 1775, 1800
AflII CTTAAG 1 cut(s) 1499
AgsI TTSAA 6 cut(s) 505, 850, 1097, 1610, 1639, 1732
AjnI CCWGG 1 cut(s) 77
AleI CACNNNNGTG 1 cut(s) 1085
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 887
AlwI GGATC 3 cut(s) 356, 554, 1400
AlwNI CAGNNNCTG 1 cut(s) 702
Ama87I CYCGRG 3 cut(s) 469, 820, 1395
AoxI GGCC 6 cut(s) 104, 369, 606, 961, 1539, 2000
ApeKI GCWGC 8 cut(s) 614, 1678, 1832, 1894, 1952, 1963, 2017, 2026
ApoI RAATTY 6 cut(s) 87, 740, 835, 914, 1441, 1737
AsiSI GCGATCGC 1 cut(s) 1354
Asp700I GAANNNNTTC 2 cut(s) 1173, 1527
AspS9I GGNCC 5 cut(s) 104, 166, 473, 606, 1539
AsuC2I CCSGG 3 cut(s) 610, 821, 822
AsuHPI GGTGA 6 cut(s) 44, 86, 359, 1415, 1520, 1998
AsuII TTCGAA 1 cut(s) 1314
AvaI CYCGRG 3 cut(s) 469, 820, 1395
AvaII GGWCC 2 cut(s) 166, 473
AxyI CCTNAGG 1 cut(s) 99
BalI TGGCCA 2 cut(s) 371, 963
BanI GGYRCC 2 cut(s) 1662, 1839
BanII GRGCYC 1 cut(s) 2049
BbsI GAAGAC 2 cut(s) 1230, 1893
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 8 cut(s) 601, 1690, 1844, 1881, 1950, 1964, 2004, 2013
BccI CCATC 4 cut(s) 191, 331, 1284, 1330
BcgI CGANNNNNNTGC 4 cut(s) 1999, 2033, 2039, 2073
BciT130I CCWGG 1 cut(s) 79
BclI TGATCA 1 cut(s) 1186
BcnI CCSGG 3 cut(s) 610, 821, 822
BcoDI GTCTC 1 cut(s) 887
BfaI CTAG 4 cut(s) 416, 1581, 1998, 2071
BfmI CTRYAG 3 cut(s) 1073, 1796, 2058
BfoI RGCGCY 3 cut(s) 1276, 1666, 1843
BfrI CTTAAG 1 cut(s) 1499
BisI GCNGC 9 cut(s) 615, 1679, 1833, 1895, 1929, 1953, 1964, 2018, 2027
BlpI GCTNAGC 1 cut(s) 2043
BlsI GCNGC 9 cut(s) 616, 1680, 1834, 1896, 1930, 1954, 1965, 2019, 2028
Bme1390I CCNGG 4 cut(s) 79, 610, 821, 822
Bme18I GGWCC 2 cut(s) 166, 473
BmeT110I CYCGRG 3 cut(s) 469, 820, 1395
BmgT120I GGNCC 5 cut(s) 104, 166, 473, 606, 1539
BmrFI CCNGG 4 cut(s) 79, 610, 821, 822
BmrI ACTGGG 1 cut(s) 1648
BmsI GCATC 8 cut(s) 763, 859, 1111, 1330, 1410, 1891, 1949, 2038
BmuI ACTGGG 1 cut(s) 1648
BpiI GAAGAC 2 cut(s) 1230, 1893
BplI GAGNNNNNCTC 2 cut(s) 59, 91
BpmI CTGGAG 2 cut(s) 52, 61
Bpu1102I GCTNAGC 1 cut(s) 2043
Bpu14I TTCGAA 1 cut(s) 1314
BpuEI CTTGAG 2 cut(s) 1280, 1343
BpuMI CCSGG 3 cut(s) 610, 821, 822
BsaHI GRCGYC 2 cut(s) 1663, 1840
BsaI GGTCTC 1 cut(s) 887
BsaJI CCNNGG 7 cut(s) 162, 186, 366, 609, 681, 820, 1542
BsaWI WCCGGW 1 cut(s) 475
BsaXI ACNNNNNCTCC 4 cut(s) 662, 692, 1905, 1935
Bsc4I CCNNNNNNNGG 6 cut(s) 436, 1261, 1581, 1659, 1775, 1800
Bse118I RCCGGY 2 cut(s) 54, 1842
Bse1I ACTGG 7 cut(s) 35, 489, 645, 1364, 1409, 1654, 1853
Bse21I CCTNAGG 1 cut(s) 99
Bse3DI GCAATG 5 cut(s) 230, 372, 724, 2012, 2021
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 7 cut(s) 162, 186, 366, 609, 681, 820, 1542
BseGI GGATG 4 cut(s) 291, 1140, 1330, 1345
BseLI CCNNNNNNNGG 6 cut(s) 436, 1261, 1581, 1659, 1775, 1800
BseMI GCAATG 5 cut(s) 230, 372, 724, 2012, 2021
BseMII CTCAG 3 cut(s) 113, 1593, 2034
BseNI ACTGG 7 cut(s) 35, 489, 645, 1364, 1409, 1654, 1853
BseRI GAGGAG 2 cut(s) 97, 1546
BseXI GCAGC 8 cut(s) 601, 1690, 1844, 1881, 1950, 1964, 2004, 2013
BsgI GTGCAG 1 cut(s) 1652
Bsh1236I CGCG 8 cut(s) 188, 333, 708, 1119, 1437, 1878, 1961, 1992
Bsh1285I CGRYCG 1 cut(s) 1354
BshFI GGCC 6 cut(s) 106, 371, 608, 963, 1541, 2002
BshNI GGYRCC 2 cut(s) 1662, 1839
BsiEI CGRYCG 1 cut(s) 1354
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 3 cut(s) 469, 820, 1395
BsiSI CCGG 5 cut(s) 55, 476, 609, 821, 1843
BslI CCNNNNNNNGG 6 cut(s) 436, 1261, 1581, 1659, 1775, 1800
BsmAI GTCTC 1 cut(s) 887
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 6 cut(s) 106, 371, 608, 963, 1541, 2002
Bso31I GGTCTC 1 cut(s) 887
BsoBI CYCGRG 3 cut(s) 469, 820, 1395
Bsp119I TTCGAA 1 cut(s) 1314
Bsp1286I GDGCHC 2 cut(s) 141, 2049
Bsp143I GATC 9 cut(s) 237, 257, 361, 546, 709, 1186, 1351, 1405, 1495
Bsp1720I GCTNAGC 1 cut(s) 2043
BspACI CCGC 8 cut(s) 26, 170, 186, 188, 304, 1437, 1929, 2040
BspANI GGCC 6 cut(s) 106, 371, 608, 963, 1541, 2002
BspCNI CTCAG 3 cut(s) 112, 1594, 2035
BspFNI CGCG 8 cut(s) 188, 333, 708, 1119, 1437, 1878, 1961, 1992
BspHI TCATGA 1 cut(s) 588
BspMAI CTGCAG 3 cut(s) 1077, 1800, 2062
BspPI GGATC 3 cut(s) 356, 554, 1400
BspQI GCTCTTC 3 cut(s) 638, 1296, 1518
BspT104I TTCGAA 1 cut(s) 1314
BspT107I GGYRCC 2 cut(s) 1662, 1839
BspTI CTTAAG 1 cut(s) 1499
BspTNI GGTCTC 1 cut(s) 887
BsrBI CCGCTC 1 cut(s) 26
BsrDI GCAATG 5 cut(s) 230, 372, 724, 2012, 2021
BsrFI RCCGGY 2 cut(s) 54, 1842
BsrI ACTGG 7 cut(s) 35, 489, 645, 1364, 1409, 1654, 1853
BssAI RCCGGY 2 cut(s) 54, 1842
BssECI CCNNGG 7 cut(s) 162, 186, 366, 609, 681, 820, 1542
BssMI GATC 9 cut(s) 237, 257, 361, 546, 709, 1186, 1351, 1405, 1495
BssNAI GTATAC 1 cut(s) 1374
BssNI GRCGYC 2 cut(s) 1663, 1840
BssT1I CCWWGG 2 cut(s) 162, 1542
Bst1107I GTATAC 1 cut(s) 1374
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 7 cut(s) 752, 1087, 1131, 1182, 1588, 1785, 1812
Bst6I CTCTTC 4 cut(s) 638, 1296, 1518, 1847
BstACI GRCGYC 2 cut(s) 1663, 1840
BstAFI CTTAAG 1 cut(s) 1499
BstAPI GCANNNNNTGC 1 cut(s) 529
BstBI TTCGAA 1 cut(s) 1314
BstC8I GCNNGC 7 cut(s) 642, 704, 778, 1667, 1676, 1844, 2040
BstDEI CTNAG 4 cut(s) 99, 950, 1602, 2043
BstDSI CCRYGG 3 cut(s) 186, 366, 681
BstEII GGTNACC 1 cut(s) 50
BstENI CCTNNNNNAGG 3 cut(s) 434, 1259, 1579
BstF5I GGATG 4 cut(s) 291, 1140, 1330, 1345
BstFNI CGCG 8 cut(s) 188, 333, 708, 1119, 1437, 1878, 1961, 1992
BstH2I RGCGCY 3 cut(s) 1276, 1666, 1843
BstKTI GATC 9 cut(s) 240, 260, 364, 549, 712, 1189, 1354, 1408, 1498
BstMAI GTCTC 1 cut(s) 887
BstMBI GATC 9 cut(s) 237, 257, 361, 546, 709, 1186, 1351, 1405, 1495
BstMCI CGRYCG 1 cut(s) 1354
BstNI CCWGG 1 cut(s) 79
BstPI GGTNACC 1 cut(s) 50
BstSCI CCNGG 4 cut(s) 77, 608, 819, 820
BstSFI CTRYAG 3 cut(s) 1073, 1796, 2058
BstUI CGCG 8 cut(s) 188, 333, 708, 1119, 1437, 1878, 1961, 1992
BstV1I GCAGC 8 cut(s) 601, 1690, 1844, 1881, 1950, 1964, 2004, 2013
BstV2I GAAGAC 2 cut(s) 1230, 1893
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
BstZ17I GTATAC 1 cut(s) 1374
Bsu36I CCTNAGG 1 cut(s) 99
BsuRI GGCC 6 cut(s) 106, 371, 608, 963, 1541, 2002
BtgI CCRYGG 3 cut(s) 186, 366, 681
BtgZI GCGATG 1 cut(s) 1134
BtsCI GGATG 4 cut(s) 291, 1140, 1330, 1345
BtsI GCAGTG 1 cut(s) 1805
BtsIMutI CAGTG 7 cut(s) 482, 719, 820, 1136, 1593, 1805, 1846
Cac8I GCNNGC 7 cut(s) 642, 704, 778, 1667, 1676, 1844, 2040
CaiI CAGNNNCTG 1 cut(s) 702
CciI TCATGA 1 cut(s) 588
Cfr10I RCCGGY 2 cut(s) 54, 1842
Cfr13I GGNCC 5 cut(s) 104, 166, 473, 606, 1539
Cfr42I CCGCGG 1 cut(s) 189
Cfr9I CCCGGG 1 cut(s) 820
CseI GACGC 1 cut(s) 867
Csp6I GTAC 5 cut(s) 264, 670, 694, 748, 1192
CviAII CATG 8 cut(s) 261, 278, 589, 637, 764, 1345, 1536, 1624
CviQI GTAC 5 cut(s) 264, 670, 694, 748, 1192
DdeI CTNAG 4 cut(s) 99, 950, 1602, 2043
DinI GGCGCC 2 cut(s) 1664, 1841
DpnI GATC 9 cut(s) 239, 259, 363, 548, 711, 1188, 1353, 1407, 1497
DpnII GATC 9 cut(s) 237, 257, 361, 546, 709, 1186, 1351, 1405, 1495
EaeI YGGCCR 2 cut(s) 369, 961
Eam1104I CTCTTC 4 cut(s) 638, 1296, 1518, 1847
EarI CTCTTC 4 cut(s) 638, 1296, 1518, 1847
Eco130I CCWWGG 2 cut(s) 162, 1542
Eco147I AGGCCT 1 cut(s) 2002
Eco24I GRGCYC 1 cut(s) 2049
Eco31I GGTCTC 1 cut(s) 887
Eco47I GGWCC 2 cut(s) 166, 473
Eco57I CTGAAG 2 cut(s) 98, 281
Eco81I CCTNAGG 1 cut(s) 99
Eco88I CYCGRG 3 cut(s) 469, 820, 1395
Eco91I GGTNACC 1 cut(s) 50
EcoNI CCTNNNNNAGG 3 cut(s) 434, 1259, 1579
EcoO65I GGTNACC 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 77
EcoT14I CCWWGG 2 cut(s) 162, 1542
EcoT22I ATGCAT 1 cut(s) 1942
EcoT38I GRGCYC 1 cut(s) 2049
EgeI GGCGCC 2 cut(s) 1664, 1841
EheI GGCGCC 2 cut(s) 1664, 1841
ErhI CCWWGG 2 cut(s) 162, 1542
FaeI CATG 8 cut(s) 264, 281, 592, 640, 767, 1348, 1539, 1627
FalI AAGNNNNNCTT 6 cut(s) 902, 934, 1215, 1247, 1515, 1547
FatI CATG 8 cut(s) 260, 277, 588, 636, 763, 1344, 1535, 1623
FauI CCCGC 2 cut(s) 177, 2047
FauNDI CATATG 1 cut(s) 985
FbaI TGATCA 1 cut(s) 1186
FblI GTMKAC 1 cut(s) 1373
Fnu4HI GCNGC 9 cut(s) 615, 1679, 1833, 1895, 1929, 1953, 1964, 2018, 2027
FokI GGATG 4 cut(s) 278, 1147, 1337, 1352
FriOI GRGCYC 1 cut(s) 2049
Fsp4HI GCNGC 9 cut(s) 615, 1679, 1833, 1895, 1929, 1953, 1964, 2018, 2027
FspBI CTAG 4 cut(s) 416, 1581, 1998, 2071
FspI TGCGCA 2 cut(s) 522, 944
GluI GCNGC 9 cut(s) 615, 1679, 1833, 1895, 1929, 1953, 1964, 2018, 2027
GsuI CTGGAG 2 cut(s) 52, 61
HaeII RGCGCY 3 cut(s) 1276, 1666, 1843
HaeIII GGCC 6 cut(s) 106, 371, 608, 963, 1541, 2002
HapII CCGG 5 cut(s) 55, 476, 609, 821, 1843
HgaI GACGC 1 cut(s) 867
Hin1I GRCGYC 2 cut(s) 1663, 1840
Hin1II CATG 8 cut(s) 264, 281, 592, 640, 767, 1348, 1539, 1627
HindIII AAGCTT 1 cut(s) 778
HinfI GANTC 2 cut(s) 68, 1174
HpaII CCGG 5 cut(s) 55, 476, 609, 821, 1843
HphI GGTGA 6 cut(s) 44, 86, 359, 1415, 1520, 1998
Hpy166II GTNNAC 3 cut(s) 956, 1080, 1374
Hpy188I TCNGA 7 cut(s) 23, 45, 117, 1028, 1685, 1723, 2034
Hpy188III TCNNGA 2 cut(s) 589, 1297
Hpy8I GTNNAC 3 cut(s) 956, 1080, 1374
Hpy99I CGWCG 1 cut(s) 1182
HpyAV CCTTC 5 cut(s) 305, 1253, 1394, 1726, 2013
HpyCH4III ACNGT 7 cut(s) 752, 1087, 1131, 1182, 1588, 1785, 1812
HpyCH4IV ACGT 2 cut(s) 688, 726
HpyF3I CTNAG 4 cut(s) 99, 950, 1602, 2043
HpySE526I ACGT 2 cut(s) 688, 726
Hsp92I GRCGYC 2 cut(s) 1663, 1840
Hsp92II CATG 8 cut(s) 264, 281, 592, 640, 767, 1348, 1539, 1627
KasI GGCGCC 2 cut(s) 1662, 1839
KroI GCCGGC 1 cut(s) 1842
KroNI GCCGGC 1 cut(s) 1844
Ksp22I TGATCA 1 cut(s) 1186
KspI CCGCGG 1 cut(s) 189
Kzo9I GATC 9 cut(s) 237, 257, 361, 546, 709, 1186, 1351, 1405, 1495
LguI GCTCTTC 3 cut(s) 638, 1296, 1518
LmnI GCTCC 5 cut(s) 23, 80, 659, 1155, 1686
Lsp1109I GCAGC 8 cut(s) 601, 1690, 1844, 1881, 1950, 1964, 2004, 2013
LweI GCATC 8 cut(s) 763, 859, 1111, 1330, 1410, 1891, 1949, 2038
MaeI CTAG 4 cut(s) 416, 1581, 1998, 2071
MaeII ACGT 2 cut(s) 688, 726
MaeIII GTNAC 7 cut(s) 50, 568, 815, 886, 1137, 1238, 1508
MalI GATC 9 cut(s) 239, 259, 363, 548, 711, 1188, 1353, 1407, 1497
MbiI CCGCTC 1 cut(s) 26
MboI GATC 9 cut(s) 237, 257, 361, 546, 709, 1186, 1351, 1405, 1495
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 2 cut(s) 141, 2049
MlsI TGGCCA 2 cut(s) 371, 963
MluNI TGGCCA 2 cut(s) 371, 963
Mly113I GGCGCC 2 cut(s) 1663, 1840
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 2 cut(s) 637, 1708
Mox20I TGGCCA 2 cut(s) 371, 963
Mph1103I ATGCAT 1 cut(s) 1942
MroNI GCCGGC 1 cut(s) 1842
MroXI GAANNNNTTC 2 cut(s) 1173, 1527
MscI TGGCCA 2 cut(s) 371, 963
MseI TTAA 5 cut(s) 831, 1485, 1500, 1764, 1986
MslI CAYNNNNRTG 3 cut(s) 155, 768, 1085
Msp20I TGGCCA 2 cut(s) 371, 963
MspA1I CMGCKG 5 cut(s) 172, 188, 1493, 1931, 2042
MspCI CTTAAG 1 cut(s) 1499
MspI CCGG 5 cut(s) 55, 476, 609, 821, 1843
MspR9I CCNGG 4 cut(s) 79, 610, 821, 822
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 1 cut(s) 79
MvnI CGCG 8 cut(s) 188, 333, 708, 1119, 1437, 1878, 1961, 1992
NaeI GCCGGC 1 cut(s) 1844
NarI GGCGCC 2 cut(s) 1663, 1840
NciI CCSGG 3 cut(s) 610, 821, 822
NdeI CATATG 1 cut(s) 985
NdeII GATC 9 cut(s) 237, 257, 361, 546, 709, 1186, 1351, 1405, 1495
NgoMIV GCCGGC 1 cut(s) 1842
NlaIII CATG 8 cut(s) 264, 281, 592, 640, 767, 1348, 1539, 1627
NmeAIII GCCGAG 1 cut(s) 558
NmuCI GTSAC 5 cut(s) 50, 815, 886, 1137, 1508
NsbI TGCGCA 2 cut(s) 522, 944
NsiI ATGCAT 1 cut(s) 1942
NspV TTCGAA 1 cut(s) 1314
OliI CACNNNNGTG 1 cut(s) 1085
PagI TCATGA 1 cut(s) 588
PceI AGGCCT 1 cut(s) 2002
PciSI GCTCTTC 3 cut(s) 638, 1296, 1518
PctI GAATGC 1 cut(s) 1152
PdiI GCCGGC 1 cut(s) 1844
PdmI GAANNNNTTC 2 cut(s) 1173, 1527
PfeI GAWTC 1 cut(s) 1174
PfoI TCCNGGA 1 cut(s) 77
PkrI GCNGC 9 cut(s) 616, 1680, 1834, 1896, 1930, 1954, 1965, 2019, 2028
Ple19I CGATCG 1 cut(s) 1354
PleI GAGTC 1 cut(s) 76
PluTI GGCGCC 2 cut(s) 1666, 1843
PpsI GAGTC 1 cut(s) 76
PsiI TTATAA 2 cut(s) 455, 512
Psp1406I AACGTT 1 cut(s) 726
Psp6I CCWGG 1 cut(s) 77
PspEI GGTNACC 1 cut(s) 50
PspGI CCWGG 1 cut(s) 77
PspPI GGNCC 5 cut(s) 104, 166, 473, 606, 1539
PstI CTGCAG 3 cut(s) 1077, 1800, 2062
PstNI CAGNNNCTG 1 cut(s) 702
PsuI RGATCY 1 cut(s) 546
PvuI CGATCG 1 cut(s) 1354
PvuII CAGCTG 1 cut(s) 1493
RgaI GCGATCGC 1 cut(s) 1354
RsaI GTAC 5 cut(s) 265, 671, 695, 749, 1193
RsaNI GTAC 5 cut(s) 264, 670, 694, 748, 1192
RseI CAYNNNNRTG 3 cut(s) 155, 768, 1085
SacII CCGCGG 1 cut(s) 189
SapI GCTCTTC 3 cut(s) 638, 1296, 1518
SaqAI TTAA 5 cut(s) 831, 1485, 1500, 1764, 1986
SatI GCNGC 9 cut(s) 615, 1679, 1833, 1895, 1929, 1953, 1964, 2018, 2027
Sau3AI GATC 9 cut(s) 237, 257, 361, 546, 709, 1186, 1351, 1405, 1495
Sau96I GGNCC 5 cut(s) 104, 166, 473, 606, 1539
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 4 cut(s) 79, 610, 821, 822
SduI GDGCHC 2 cut(s) 141, 2049
SfaAI GCGATCGC 1 cut(s) 1354
SfaNI GCATC 8 cut(s) 763, 859, 1111, 1330, 1410, 1891, 1949, 2038
SfcI CTRYAG 3 cut(s) 1073, 1796, 2058
SfoI GGCGCC 2 cut(s) 1664, 1841
Sfr303I CCGCGG 1 cut(s) 189
SfuI TTCGAA 1 cut(s) 1314
SgfI GCGATCGC 1 cut(s) 1354
SgrBI CCGCGG 1 cut(s) 189
SinI GGWCC 2 cut(s) 166, 473
SmaI CCCGGG 1 cut(s) 822
SmiMI CAYNNNNRTG 3 cut(s) 155, 768, 1085
SmlI CTYRAG 3 cut(s) 1295, 1358, 1499
SmoI CTYRAG 3 cut(s) 1295, 1358, 1499
SseBI AGGCCT 1 cut(s) 2002
SsiI CCGC 8 cut(s) 26, 170, 186, 188, 304, 1437, 1929, 2040
SspDI GGCGCC 2 cut(s) 1662, 1839
SspI AATATT 1 cut(s) 1002
SspMI CTAG 4 cut(s) 416, 1581, 1998, 2071
StuI AGGCCT 1 cut(s) 2002
StyD4I CCNGG 4 cut(s) 77, 608, 819, 820
StyI CCWWGG 2 cut(s) 162, 1542
TaaI ACNGT 7 cut(s) 752, 1087, 1131, 1182, 1588, 1785, 1812
TaiI ACGT 2 cut(s) 691, 729
TaqI TCGA 4 cut(s) 256, 1177, 1314, 1863
TatI WGTACW 3 cut(s) 693, 747, 1191
TauI GCSGC 1 cut(s) 1931
TfiI GAWTC 1 cut(s) 1174
Tru1I TTAA 5 cut(s) 831, 1485, 1500, 1764, 1986
Tru9I TTAA 5 cut(s) 831, 1485, 1500, 1764, 1986
TscAI CASTG 7 cut(s) 489, 719, 820, 1136, 1593, 1805, 1853
TseFI GTSAC 5 cut(s) 50, 815, 886, 1137, 1508
TseI GCWGC 8 cut(s) 614, 1678, 1832, 1894, 1952, 1963, 2017, 2026
Tsp45I GTSAC 5 cut(s) 50, 815, 886, 1137, 1508
TspGWI ACGGA 3 cut(s) 670, 905, 1391
TspMI CCCGGG 1 cut(s) 820
TspRI CASTG 7 cut(s) 489, 719, 820, 1136, 1593, 1805, 1853
Vha464I CTTAAG 1 cut(s) 1499
VpaK11BI GGWCC 2 cut(s) 166, 473
XagI CCTNNNNNAGG 3 cut(s) 434, 1259, 1579
XapI RAATTY 6 cut(s) 87, 740, 835, 914, 1441, 1737
XmaI CCCGGG 1 cut(s) 820
XmiI GTMKAC 1 cut(s) 1373
XmnI GAANNNNTTC 2 cut(s) 1173, 1527
XspI CTAG 4 cut(s) 416, 1581, 1998, 2071
Zsp2I ATGCAT 1 cut(s) 1942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.