Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006016

Physical Location & Seq

Genomic Coordinates Forward (+)

18986922 .. 18991504

Gene Structure 4583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1083400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2082 bp

ATGGCACACCTCGAGTGTGACTCCTCGATTTCTCGTGCGGAAACAGGTTTGGGAGGTGATGATCTGTATACAGAGCTATGGAAGTTGTGTGCAGGGCCTCTGGTGGATGTACCAAGGCCTGGAGATAAGGTCTTCTACTTTCCTCAAGGTCATATGGAACAACTGGAAGCATCAACAAATCAGGAACTGAACCAGCAAATCCCTCTCTTTAATCTTCCCTCAAAGATCCTTTGTAGTGTGGTCAATATTCGATTACTGGCAGAACAAGAAACAGATGAGGTTTATGCTCAAATCACTTTGCATCCAGAAGCAGATCAAAGTGAACCTACAAGTCCTGATCCATGCAAACCCGAAGCTCCAAAGCCAACGGTTCACTGGTTTTGCAAGATTTTGACAGCTTCCGATACAAGCACCCATGGAGGGTTCTCAGTCCTTCGAAAGCATGCCACCGAGTGCTTACCTCCGTTGGACATGAACCAAGCAACTCCAACTCAGGAGTTAATCGCCAAAGATCTTCATGGGTATGAGTGGAAATTTAAGCACATCTTTAGAGGTCAACCGCGGAGGCATTTACTTACCACAGGATGGAGTACATTCGTCACTTCAAAAAGATTGGTTGCTGGTGACGCCTTTGTGTTTTTGAGGGGTGACAATGGGGAATTACGGGTTGGGGTTCGGCGTCTAGCTCGTCAACAGAGTCAGATGCCATTATCTGTGATATCAAGCCAGAGCATGCATCTTGGAGTGCTTGCAACTGCTTCGCATGCTCTTATGACCAAAACACTGTTTGTTGTGTATTCCAAGCCAAGGACTAGCCAGTTCATTGTTTGCTTAAACAAATATCTGGAGGCCATCAACAATAAATTCTCGCTTGGGATGCGCTTCAGGATGCGATTTGAGGGAGAAGAATCTCCTGAAAGAAGGTTCACAGGCACAATAGTTGAGGTTGGGGATTTATCTCCTCAGTGGTTGGAGTCTAAATGGCGATCGTTGAAGGTTCAATGGGATGAGCATGCTGCAGTTCAAAGGCCAGAGAGGGTTTCTCCTTGGGATATTGAACTTTTTGTAGCTTCTGCTCCTTTAAATCTTGCTCAACCAGTGGTAAAGAGTAAAAGGCCTCGGCCTGTAGAAATTGCTTCATCTGAAGTTACCACCAATTCAGCTGCTTCAGCATTTTGGTATCATAGCTCACCCCAGACCACAGAGTTAAACCAATTAGGTGGTGTTCCGGAAGTCCAAAGCTCTAGTAGTCAGATTGTCTGGCCTATGAGGCAGAAAGAAAGCAATAGCAGCAGTTACTCTAGCGCAAGGGTATGCTCAGATGGCATCTGGCCTTCTTCCCCGAACGTGACTGTTTCTTTGTGCCTTTTCCCAGACTCAAAGGAAAGCAACAAGAATGTAACTATAGGGTCTGTGCTCTCTAGCTTTGCCTCCCCAATTACCTCAAAGCCAAATAATGTCCCGATGCATGACCAGGTTGAAAACGGGAAAAAATCTGACACTTCAGGTTTTAGGTTGTTTGGTTGCAATTTGACAAACAACACTAAAACAACTTGTCCTCAAGAGCTAGTGTTTAAAACAATGCCGTCTGGTGCCAAAGGACCTATTCCAGCTGATGCATTTGAATCCGATCAGATTCTGGATGTTTCGAAGCTGTCAAAAGAGCAGAAACAAGTAATCTTGGAGGCCTCACCAAAAGAAACACAGGGCAAGCAAGGATTGACTCTTTCAATGAGAACTCGCACTAAGGTGCAAATGCAAGGGGTTGCTGTTGGTCGTGCTTTGGACTTGACTGCATTGAAAGGCTACGATCATCTAATTGATGAGCTAGAGAAAATGTTTGAGATCAAAGGGGAGCTTCGTCCACAGAACAAATGGGCAGTTGTTTTCACGGATGATGAAAATGACATGATGCTTATGGGCGATGATCAATGGCCAGACTTCTGTAAGCTGGTGAAGAAGATATTTATATATTCAAGCGACGAGGTACAGAAGATGAACAGATTCAAGCTTCAGAGTTCATCTTTAGATTGTGAAGGGACGGTAAGCATGGATTCTGAGCGTAGATTGGAACATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006355 GO:0008150 GO:0009506 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030054 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0055044 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

694

Amino Acids

77.57

Weight (kDa)

6.29

Isoelectric Point (pI)

49.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	127 - 227	2.2e-22	B3 DNA binding domain
BRWD_AD	PF25313	260 - 353	2.2e-09	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	274 - 355	2.9e-34	Auxin response factor ancillary domain
AUX_IAA	PF02309	568 - 662	3.7e-06	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006016)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46530 AT2G46530 AT2G46530 AT3G61830 AT3G61830
fragaria_vesca	FvH4_7g16840 FvH4_7g16840 FvH4_7g16840 FvH4_7g16840 FvH4_7g16840 FvH4_7g16840
malus_domestica	MD01G1083400.v1.1 MD07G1152100.v1.1
prunus_persica	Prupe.2G190400_v2.0.a1 Prupe.2G190400_v2.0.a1 Prupe.2G190400_v2.0.a1
pyrus_communis	pycom07g15010
rosa_chinensis	RchiOBHm_Chr1g0360041
rosa_laevigata	RLG00000027836
rosa_multiflora	Rmu_ssc0000368.1_g000035
rosa_roxburghii	Rroxscaffold_4G00296430
rosa_rugosa	Rorug01G0274600
rosa_samantha	Rh1AG288600 Rh1BG254100 Rh1CG271600 Rh1DG283200
rosa_wichuraiana	Rw1G025590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1592
AccB7I	CCANNNNNTGG	2 cut(s)	119, 585
AccI	GTMKAC	1 cut(s)	68
AccII	CGCG	1 cut(s)	562
AccIII	TCCGGA	1 cut(s)	1228
AciI	CCGC	3 cut(s)	38, 560, 562
AclWI	GGATC	2 cut(s)	220, 332
AcoI	YGGCCR	1 cut(s)	1934
AcsI	RAATTY	2 cut(s)	533, 863
AcuI	CTGAAG	5 cut(s)	868, 1152, 1164, 1488, 1997
AcyI	GRCGYC	2 cut(s)	627, 679
AdeI	CACNNNGTG	1 cut(s)	453
AfaI	GTAC	3 cut(s)	111, 592, 1989
AfiI	CCNNNNNNNGG	4 cut(s)	119, 420, 585, 807
AjnI	CCWGG	2 cut(s)	118, 1473
AjuI	GAANNNNNNNTTGG	2 cut(s)	651, 683
AleI	CACNNNNGTG	1 cut(s)	1748
Alw21I	GWGCWC	1 cut(s)	1419
AlwI	GGATC	2 cut(s)	220, 332
AlwNI	CAGNNNCTG	2 cut(s)	187, 1639
Ama87I	CYCGRG	1 cut(s)	11
Aor13HI	TCCGGA	1 cut(s)	1228
ApeKI	GCWGC	3 cut(s)	1016, 1163, 1290
ApoI	RAATTY	2 cut(s)	533, 863
ArsI	GACNNNNNNTTYG	2 cut(s)	1342, 1374
Asp700I	GAANNNNTTC	1 cut(s)	2003
AspLEI	GCGC	2 cut(s)	882, 1307
AspS9I	GGNCC	2 cut(s)	95, 1601
AsuHPI	GGTGA	6 cut(s)	68, 635, 659, 1182, 1683, 1966
AsuII	TTCGAA	2 cut(s)	436, 1649
AvaI	CYCGRG	1 cut(s)	11
AvaII	GGWCC	1 cut(s)	1601
BalI	TGGCCA	1 cut(s)	1936
BanI	GGYRCC	1 cut(s)	1592
BauI	CACGAG	1 cut(s)	33
BbsI	GAAGAC	1 cut(s)	124
Bbv12I	GWGCWC	1 cut(s)	1419
BbvI	GCAGC	3 cut(s)	1003, 1150, 1302
BccI	CCATC	3 cut(s)	579, 860, 1316
BceAI	ACGGC	1 cut(s)	1570
BcgI	CGANNNNNNTGC	2 cut(s)	741, 775
BciT130I	CCWGG	2 cut(s)	120, 1475
BclI	TGATCA	1 cut(s)	1927
BfaI	CTAG	7 cut(s)	683, 813, 1245, 1302, 1422, 1568, 1829
BfmI	CTRYAG	3 cut(s)	1017, 1125, 1404
BglI	GCCNNNNNGGC	1 cut(s)	1270
BglII	AGATCT	1 cut(s)	511
BisI	GCNGC	3 cut(s)	1017, 1164, 1291
BlsI	GCNGC	3 cut(s)	1018, 1165, 1292
Bme1390I	CCNGG	2 cut(s)	120, 1475
Bme18I	GGWCC	1 cut(s)	1601
BmeT110I	CYCGRG	1 cut(s)	11
BmgT120I	GGNCC	2 cut(s)	95, 1601
BmiI	GGNNCC	1 cut(s)	1594
BmrFI	CCNGG	2 cut(s)	120, 1475
BpiI	GAAGAC	1 cut(s)	124
BplI	GAGNNNNNCTC	3 cut(s)	37, 1027, 1059
BpmI	CTGGAG	2 cut(s)	141, 866
Bpu14I	TTCGAA	2 cut(s)	436, 1649
BpuEI	CTTGAG	2 cut(s)	129, 1545
BsaHI	GRCGYC	2 cut(s)	627, 679
BsaJI	CCNNGG	6 cut(s)	113, 415, 560, 806, 1046, 1118
BsaWI	WCCGGW	1 cut(s)	1228
BsaXI	ACNNNNNCTCC	2 cut(s)	114, 144
Bsc4I	CCNNNNNNNGG	4 cut(s)	119, 420, 585, 807
Bse1I	ACTGG	5 cut(s)	168, 261, 380, 817, 1097
BseAI	TCCGGA	1 cut(s)	1228
BseBI	CCWGG	2 cut(s)	120, 1475
BseDI	CCNNGG	6 cut(s)	113, 415, 560, 806, 1046, 1118
BseGI	GGATG	8 cut(s)	112, 301, 590, 882, 894, 1012, 1648, 1900
BseLI	CCNNNNNNNGG	4 cut(s)	119, 420, 585, 807
BseMII	CTCAG	5 cut(s)	441, 506, 977, 1332, 2049
BseNI	ACTGG	5 cut(s)	168, 261, 380, 817, 1097
BseRI	GAGGAG	2 cut(s)	13, 951
BseXI	GCAGC	3 cut(s)	1003, 1150, 1302
BsgI	GTGCAG	1 cut(s)	111
Bsh1236I	CGCG	1 cut(s)	562
Bsh1285I	CGRYCG	1 cut(s)	989
BshNI	GGYRCC	1 cut(s)	1592
BsiEI	CGRYCG	1 cut(s)	989
BsiHKAI	GWGCWC	1 cut(s)	1419
BsiHKCI	CYCGRG	1 cut(s)	11
BsiSI	CCGG	1 cut(s)	1229
BslFI	GGGAC	2 cut(s)	1445, 2053
BslI	CCNNNNNNNGG	4 cut(s)	119, 420, 585, 807
BsmFI	GGGAC	2 cut(s)	1445, 2053
BsoBI	CYCGRG	1 cut(s)	11
Bsp119I	TTCGAA	2 cut(s)	436, 1649
Bsp1286I	GDGCHC	1 cut(s)	1419
Bsp13I	TCCGGA	1 cut(s)	1228
Bsp19I	CCATGG	1 cut(s)	415
BspACI	CCGC	3 cut(s)	38, 560, 562
BspCNI	CTCAG	5 cut(s)	440, 505, 976, 1331, 2050
BspEI	TCCGGA	1 cut(s)	1228
BspFNI	CGCG	1 cut(s)	562
BspLI	GGNNCC	1 cut(s)	1594
BspMAI	CTGCAG	1 cut(s)	1021
BspPI	GGATC	2 cut(s)	220, 332
BspT104I	TTCGAA	2 cut(s)	436, 1649
BspT107I	GGYRCC	1 cut(s)	1592
BsrI	ACTGG	5 cut(s)	168, 261, 380, 817, 1097
BssECI	CCNNGG	6 cut(s)	113, 415, 560, 806, 1046, 1118
BssNAI	GTATAC	1 cut(s)	69
BssNI	GRCGYC	2 cut(s)	627, 679
BssSI	CACGAG	1 cut(s)	33
BssT1I	CCWWGG	4 cut(s)	113, 415, 806, 1046
Bst1107I	GTATAC	1 cut(s)	69
Bst2BI	CACGAG	1 cut(s)	33
Bst2UI	CCWGG	2 cut(s)	120, 1475
Bst4CI	ACNGT	4 cut(s)	370, 786, 1354, 2044
BstACI	GRCGYC	2 cut(s)	627, 679
BstBI	TTCGAA	2 cut(s)	436, 1649
BstC8I	GCNNGC	6 cut(s)	444, 734, 750, 765, 1014, 1712
BstDEI	CTNAG	6 cut(s)	427, 492, 963, 1318, 1746, 2058
BstDSI	CCRYGG	2 cut(s)	415, 560
BstF5I	GGATG	8 cut(s)	112, 301, 590, 882, 894, 1012, 1648, 1900
BstFNI	CGCG	1 cut(s)	562
BstHHI	GCGC	2 cut(s)	882, 1307
BstMCI	CGRYCG	1 cut(s)	989
BstMWI	GCNNNNNNNGC	7 cut(s)	626, 764, 877, 1169, 1270, 1290, 1323
BstNI	CCWGG	2 cut(s)	120, 1475
BstNSI	RCATGY	4 cut(s)	446, 736, 767, 1016
BstSCI	CCNGG	2 cut(s)	118, 1473
BstSFI	CTRYAG	3 cut(s)	1017, 1125, 1404
BstUI	CGCG	1 cut(s)	562
BstV1I	GCAGC	3 cut(s)	1003, 1150, 1302
BstV2I	GAAGAC	1 cut(s)	124
BstX2I	RGATCY	2 cut(s)	225, 511
BstXI	CCANNNNNNTGG	1 cut(s)	1220
BstYI	RGATCY	2 cut(s)	225, 511
BstZ17I	GTATAC	1 cut(s)	69
BtgI	CCRYGG	2 cut(s)	415, 560
BtgZI	GCGATG	1 cut(s)	1938
BtsCI	GGATG	8 cut(s)	112, 301, 590, 882, 894, 1012, 1648, 1900
BtsIMutI	CAGTG	4 cut(s)	373, 782, 971, 1104
Cac8I	GCNNGC	6 cut(s)	444, 734, 750, 765, 1014, 1712
CaiI	CAGNNNCTG	2 cut(s)	187, 1639
CfoI	GCGC	2 cut(s)	882, 1307
Cfr13I	GGNCC	2 cut(s)	95, 1601
Cfr42I	CCGCGG	1 cut(s)	563
CseI	GACGC	2 cut(s)	635, 668
CsiI	ACCWGGT	1 cut(s)	1473
Csp6I	GTAC	3 cut(s)	110, 591, 1988
CviQI	GTAC	3 cut(s)	110, 591, 1988
DdeI	CTNAG	6 cut(s)	427, 492, 963, 1318, 1746, 2058
DraI	TTTAAA	2 cut(s)	1083, 1576
DraIII	CACNNNGTG	1 cut(s)	453
EaeI	YGGCCR	1 cut(s)	1934
Eco130I	CCWWGG	4 cut(s)	113, 415, 806, 1046
Eco147I	AGGCCT	3 cut(s)	118, 1117, 1688
Eco32I	GATATC	1 cut(s)	720
Eco47I	GGWCC	1 cut(s)	1601
Eco57I	CTGAAG	5 cut(s)	868, 1152, 1164, 1488, 1997
Eco88I	CYCGRG	1 cut(s)	11
EcoO109I	RGGNCCY	2 cut(s)	95, 1601
EcoRII	CCWGG	2 cut(s)	118, 1473
EcoRV	GATATC	1 cut(s)	720
EcoT14I	CCWWGG	4 cut(s)	113, 415, 806, 1046
EcoT22I	ATGCAT	3 cut(s)	738, 1470, 1621
ErhI	CCWWGG	4 cut(s)	113, 415, 806, 1046
FalI	AAGNNNNNCTT	4 cut(s)	530, 562, 1842, 1874
FaqI	GGGAC	2 cut(s)	1445, 2053
FauNDI	CATATG	1 cut(s)	153
FbaI	TGATCA	1 cut(s)	1927
FblI	GTMKAC	1 cut(s)	68
Fnu4HI	GCNGC	3 cut(s)	1017, 1164, 1291
FokI	GGATG	8 cut(s)	119, 288, 597, 889, 901, 1019, 1655, 1907
Fsp4HI	GCNGC	3 cut(s)	1017, 1164, 1291
FspBI	CTAG	7 cut(s)	683, 813, 1245, 1302, 1422, 1568, 1829
GlaI	GCGC	2 cut(s)	881, 1306
GluI	GCNGC	3 cut(s)	1017, 1164, 1291
GsuI	CTGGAG	2 cut(s)	141, 866
HapII	CCGG	1 cut(s)	1229
HgaI	GACGC	2 cut(s)	635, 668
HhaI	GCGC	2 cut(s)	882, 1307
Hin1I	GRCGYC	2 cut(s)	627, 679
Hin6I	GCGC	2 cut(s)	880, 1305
HinP1I	GCGC	2 cut(s)	880, 1305
HincII	GTYRAC	2 cut(s)	557, 692
HindII	GTYRAC	2 cut(s)	557, 692
HindIII	AAGCTT	1 cut(s)	2009
HpaII	CCGG	1 cut(s)	1229
HphI	GGTGA	6 cut(s)	68, 635, 659, 1182, 1683, 1966
Hpy166II	GTNNAC	7 cut(s)	69, 323, 373, 557, 692, 927, 1865
Hpy8I	GTNNAC	7 cut(s)	69, 323, 373, 557, 692, 927, 1865
Hpy99I	CGWCG	1 cut(s)	1985
HpyAV	CCTTC	5 cut(s)	443, 915, 988, 1344, 2030
HpyCH4III	ACNGT	4 cut(s)	370, 786, 1354, 2044
HpyCH4IV	ACGT	1 cut(s)	1347
HpyF10VI	GCNNNNNNNGC	7 cut(s)	626, 764, 877, 1169, 1270, 1290, 1323
HpyF3I	CTNAG	6 cut(s)	427, 492, 963, 1318, 1746, 2058
HpySE526I	ACGT	1 cut(s)	1347
Hsp92I	GRCGYC	2 cut(s)	627, 679
HspAI	GCGC	2 cut(s)	880, 1305
Kpn2I	TCCGGA	1 cut(s)	1228
Ksp22I	TGATCA	1 cut(s)	1927
KspI	CCGCGG	1 cut(s)	563
LmnI	GCTCC	3 cut(s)	361, 1081, 1855
Lsp1109I	GCAGC	3 cut(s)	1003, 1150, 1302
MabI	ACCWGGT	1 cut(s)	1473
MaeI	CTAG	7 cut(s)	683, 813, 1245, 1302, 1422, 1568, 1829
MaeII	ACGT	1 cut(s)	1347
MaeIII	GTNAC	8 cut(s)	17, 598, 623, 647, 1147, 1295, 1348, 1399
MboII	GAAGA	8 cut(s)	124, 206, 506, 917, 1329, 1969, 1972, 2005
MflI	RGATCY	2 cut(s)	225, 511
MhlI	GDGCHC	1 cut(s)	1419
MlsI	TGGCCA	1 cut(s)	1936
MluCI	AATT	9 cut(s)	533, 659, 863, 1131, 1156, 1214, 1437, 1528, 1818
MluNI	TGGCCA	1 cut(s)	1936
MlyI	GAGTC	5 cut(s)	14, 706, 983, 1370, 1717
MmeI	TCCRAC	3 cut(s)	447, 512, 951
Mox20I	TGGCCA	1 cut(s)	1936
Mph1103I	ATGCAT	3 cut(s)	738, 1470, 1621
MroI	TCCGGA	1 cut(s)	1228
MroXI	GAANNNNTTC	1 cut(s)	2003
MscI	TGGCCA	1 cut(s)	1936
MseI	TTAA	7 cut(s)	210, 500, 537, 833, 1082, 1208, 1575
MslI	CAYNNNNRTG	2 cut(s)	522, 1748
Msp20I	TGGCCA	1 cut(s)	1936
MspA1I	CMGCKG	3 cut(s)	562, 1163, 1613
MspI	CCGG	1 cut(s)	1229
MspR9I	CCNGG	2 cut(s)	120, 1475
MvaI	CCWGG	2 cut(s)	120, 1475
MvnI	CGCG	1 cut(s)	562
MwoI	GCNNNNNNNGC	7 cut(s)	626, 764, 877, 1169, 1270, 1290, 1323
NcoI	CCATGG	1 cut(s)	415
NdeI	CATATG	1 cut(s)	153
NlaIV	GGNNCC	1 cut(s)	1594
NmeAIII	GCCGAG	1 cut(s)	1099
NmuCI	GTSAC	5 cut(s)	17, 598, 623, 647, 1348
NsiI	ATGCAT	3 cut(s)	738, 1470, 1621
NspI	RCATGY	4 cut(s)	446, 736, 767, 1016
NspV	TTCGAA	2 cut(s)	436, 1649
OliI	CACNNNNGTG	1 cut(s)	1748
PaeI	GCATGC	4 cut(s)	446, 736, 767, 1016
PaeR7I	CTCGAG	1 cut(s)	11
PceI	AGGCCT	3 cut(s)	118, 1117, 1688
PdmI	GAANNNNTTC	1 cut(s)	2003
PfeI	GAWTC	5 cut(s)	908, 1625, 1636, 2004, 2054
PflMI	CCANNNNNTGG	2 cut(s)	119, 585
PkrI	GCNGC	3 cut(s)	1018, 1165, 1292
Ple19I	CGATCG	1 cut(s)	989
PleI	GAGTC	5 cut(s)	14, 705, 982, 1370, 1717
PpsI	GAGTC	5 cut(s)	14, 705, 982, 1370, 1717
PpuMI	RGGWCCY	1 cut(s)	1601
Psp5II	RGGWCCY	1 cut(s)	1601
Psp6I	CCWGG	2 cut(s)	118, 1473
PspGI	CCWGG	2 cut(s)	118, 1473
PspN4I	GGNNCC	1 cut(s)	1594
PspPI	GGNCC	2 cut(s)	95, 1601
PspPPI	RGGWCCY	1 cut(s)	1601
PspXI	VCTCGAGB	1 cut(s)	11
PstI	CTGCAG	1 cut(s)	1021
PstNI	CAGNNNCTG	2 cut(s)	187, 1639
PsuI	RGATCY	2 cut(s)	225, 511
PvuI	CGATCG	1 cut(s)	989
PvuII	CAGCTG	2 cut(s)	1163, 1613
RsaI	GTAC	3 cut(s)	111, 592, 1989
RsaNI	GTAC	3 cut(s)	110, 591, 1988
RseI	CAYNNNNRTG	2 cut(s)	522, 1748
SacII	CCGCGG	1 cut(s)	563
SaqAI	TTAA	7 cut(s)	210, 500, 537, 833, 1082, 1208, 1575
SatI	GCNGC	3 cut(s)	1017, 1164, 1291
Sau96I	GGNCC	2 cut(s)	95, 1601
SchI	GAGTC	5 cut(s)	14, 706, 983, 1370, 1717
ScrFI	CCNGG	2 cut(s)	120, 1475
SduI	GDGCHC	1 cut(s)	1419
SexAI	ACCWGGT	1 cut(s)	1473
SfcI	CTRYAG	3 cut(s)	1017, 1125, 1404
Sfr274I	CTCGAG	1 cut(s)	11
Sfr303I	CCGCGG	1 cut(s)	563
SfuI	TTCGAA	2 cut(s)	436, 1649
SgrBI	CCGCGG	1 cut(s)	563
SinI	GGWCC	1 cut(s)	1601
SlaI	CTCGAG	1 cut(s)	11
SmiMI	CAYNNNNRTG	2 cut(s)	522, 1748
SmlI	CTYRAG	3 cut(s)	11, 144, 1560
SmoI	CTYRAG	3 cut(s)	11, 144, 1560
SphI	GCATGC	4 cut(s)	446, 736, 767, 1016
Sse9I	AATT	9 cut(s)	533, 659, 863, 1131, 1156, 1214, 1437, 1528, 1818
SseBI	AGGCCT	3 cut(s)	118, 1117, 1688
SsiI	CCGC	3 cut(s)	38, 560, 562
SspI	AATATT	1 cut(s)	247
SspMI	CTAG	7 cut(s)	683, 813, 1245, 1302, 1422, 1568, 1829
StuI	AGGCCT	3 cut(s)	118, 1117, 1688
StyD4I	CCNGG	2 cut(s)	118, 1473
StyI	CCWWGG	4 cut(s)	113, 415, 806, 1046
TaaI	ACNGT	4 cut(s)	370, 786, 1354, 2044
TaiI	ACGT	1 cut(s)	1350
TaqI	TCGA	5 cut(s)	12, 26, 250, 436, 1649
TasI	AATT	9 cut(s)	533, 659, 863, 1131, 1156, 1214, 1437, 1528, 1818
TatI	WGTACW	1 cut(s)	590
TfiI	GAWTC	5 cut(s)	908, 1625, 1636, 2004, 2054
Tru1I	TTAA	7 cut(s)	210, 500, 537, 833, 1082, 1208, 1575
Tru9I	TTAA	7 cut(s)	210, 500, 537, 833, 1082, 1208, 1575
TscAI	CASTG	4 cut(s)	380, 789, 971, 1104
TseFI	GTSAC	5 cut(s)	17, 598, 623, 647, 1348
TseI	GCWGC	3 cut(s)	1016, 1163, 1290
Tsp45I	GTSAC	5 cut(s)	17, 598, 623, 647, 1348
TspDTI	ATGAA	7 cut(s)	488, 506, 811, 1128, 1914, 2010, 2012
TspGWI	ACGGA	2 cut(s)	453, 1907
TspRI	CASTG	4 cut(s)	380, 789, 971, 1104
Van91I	CCANNNNNTGG	2 cut(s)	119, 585
VpaK11BI	GGWCC	1 cut(s)	1601
XapI	RAATTY	2 cut(s)	533, 863
XceI	RCATGY	4 cut(s)	446, 736, 767, 1016
XcmI	CCANNNNNNNNNTGG	2 cut(s)	372, 1872
XhoI	CTCGAG	1 cut(s)	11
XmiI	GTMKAC	1 cut(s)	68
XmnI	GAANNNNTTC	1 cut(s)	2003
XspI	CTAG	7 cut(s)	683, 813, 1245, 1302, 1422, 1568, 1829
Zsp2I	ATGCAT	3 cut(s)	738, 1470, 1621

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.