Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008253

Physical Location & Seq

Genomic Coordinates Reverse (-)

21448235 .. 21452084

Gene Structure 3850 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1101700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGAGAGCTTTGAGCTCGCAATGCGTGCTCGTTGATTTGCAGAGCTCATGGCAATCGGCGAACCAGATCCCAATCTCGACCCTCTGCTACCTTCAGAACTCCAAATTCACCTCTGCAGTCTCTTCGGCATTTCGTCGAACTCGCGGTGGGCGTAAGCCGGTTGCTTCGTCCAAATCCTCCACCCCATGGATTCCGGCCCCGGAGATTCGGAGGCCGTCCTATCGGTTCTATTCCGGAAATGGGTTGGTTTCAAATTCTCCGAATTTGAATCCGGGCTCCACGTCGCAGCCGGAGGTGGCTTCGGAGCTGGGAATGTTACTTGAATTGTTGCCTTTGAGGATGAGGAGTGAGCTGTACAGGCACGAGGAGATTGGCGAGCTCGTTGAGGTTGTGATGGATTTGGGGAGGAGTCCGATTGCGAGGTTTCCTTCCGGTGATTGGGTGATGTCCGAAAAGCCGGTGAATCATGAAGATCTACGGCACGCTGTATCGAAGGTTAGTGAGTTTTCAGATGACAACCGTTCAGGTATTAACAATTCACTACACCGGATAAGTGCTATTCGAAACCGGAAAATGCAAATCATTGGCCTCACGTGCCGAGTGGGACGAGCTGTGTCAGGAAGCGCTGAGATCATACGTGACTTGGTTGAGGGCGGCGGTTCCATCTTGGTCATTGGACCTCCTGGAGTTGGGAAGACAACCTTAATCAGAGAAATTGCTAGAATGCTGGCAGATGATCATTTGAAGCGTGTTGTGATTGTGGATACATCTAATGAGATTGGTGGAGACGGAGATGTTCCTCACGCAGGAATAGGTCGTGCAAGGAGGATGCAAGTTCCTAATGTCAATATGCAGCATAATGTTATGATTGAAGCAGTGGAAAACCATATGCCCGAAACAATCATAATAGATGAAATTGGAACAGAACTTGAAGCATTGGCTGCCAGTACTATTGCTCAAAGAGGAGTCCAACTAGTTGGAACAGCACATGGAATGACGATAGACAACGTAATGAAAAATCCTTCTCTACAGATCCTTGTCGGTGGCATAGAGAGTGTGACACTTGGTGATGAAGAAGCAAGAAGAAGGAAGGTACAGAAGACAATTCTGGAAAGAAGGGGGCCTCCTACATTTACATGTGCTGTGGAGATTATATCTAAAACAGAACTTCGTGTGCATCACAGATTAGATGCAACTGTTGATGCTATACTGGCTGGAAAATCTCCTTTGTTTGAAATCCGTCATGTTGATGCAGATGCTAGTGGCTCTCGCAAGTCTATTTCAATACCTGAAAAGAGCTATCTACAAGTGTCTGATTTTACTGCTAAAAAGAACATTGATGCCGCTGATATAGAGTCTGACAAGGAAGATCTAGACCATTCTCCTACACGGTCCAAAAAATTGAGCAGCAAAAGATCTGTGAAGAAGAGGAGTTTGCCGGTTTGTGTATATGCATACAAGATCCTTGAAGCCGATCTTCTACAAGTAGCAAAGGTAATGGGGCTTGAAGATGAAATAGAAGTAACTGATGATATCGGAACAGCTGATGTAATAATAGCATCCAGTTATGAAATGAAACAGAGCCCATGGATCCGCAGTGTAACAAAGTTCCACGACTTGCCTGTATTTGTCATGAAGTCAAATACCATGGCACAAATGGTCAAGGCAGTTCGGATGATTCTTGAAATGGAATCTGTTGGCTCTTTGCGAAAGCAGTTAGTCAATAGTTCTTTCGACATTGAAATTGAAGATGATGCCCCGAAAAGAAAACCATCTTTGGAAGAGATTGATGCATTGGAGGAGGTTCGACTTGCGATTGAGTACATCGTGATTCCTGGTGGAGAGCCAGTCGAGCTTCTCCCAAGACGCACTGAAATAATCGCCCGACAGCTTGAGCTAGTGGAAAGTTATCAGCTGGCTGCCGAAAATTCGGGCACCGAGATGAATCCGAGGTTGCAGATTCTTCCGATGAGATTAAACAAAAAGAAAGCTTCAAAACCTGTTAAATCCGGTTCAAGTTTTCTGGAGGTGATTAACTCTAAATCACCAACCGGCGGAGGGGGAGGAACCAGTGTTGCTAGGCTGCCTCTTATATCTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA

Protein Analysis

701

Amino Acids

76.93

Weight (kDa)

6.95

Isoelectric Point (pI)

57.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spore_III_AA	PF19568	194 - 347	1.1e-12	Sporulation stage III, protein AA
PTPase	PF25516	481 - 561	8.1e-26	Phosphotyrosine protein phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008253)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10420 AT3G10420
fragaria_vesca	FvH4_7g18530 FvH4_7g18530 FvH4_7g18530
malus_domestica	MD01G1101700.v1.1 MD07G1168300.v1.1
prunus_persica	Prupe.2G206900_v2.0.a1
pyrus_communis	pycom01g12830 pycom07g16270
rosa_chinensis	RchiOBHm_Chr1g0362311
rosa_laevigata	RLG00000027652
rosa_multiflora	Rmu_sc0013178.1_g000001 Rmu_sc0037182.1_g000001
rosa_roxburghii	Rroxscaffold_4G00294260
rosa_rugosa	Rorug01G0297300.1 Rorug01G0297400 Rorug01G0297500
rosa_samantha	Rh1AG306200 Rh1BG269700 Rh1CG287200 Rh1DG299900
rosa_wichuraiana	Rw1G027180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1934
AccB7I	CCANNNNNTGG	1 cut(s)	186
AccII	CGCG	1 cut(s)	144
AccIII	TCCGGA	1 cut(s)	233
AciI	CCGC	6 cut(s)	144, 654, 657, 1344, 1594, 2055
AclWI	GGATC	5 cut(s)	61, 1027, 1456, 1585, 1598
AcsI	RAATTY	4 cut(s)	104, 253, 262, 1927
AcuI	CTGAAG	1 cut(s)	77
AcvI	CACGTG	1 cut(s)	594
AdeI	CACNNNGTG	1 cut(s)	1067
AfaI	GTAC	4 cut(s)	356, 949, 1095, 1823
AfeI	AGCGCT	1 cut(s)	625
AfiI	CCNNNNNNNGG	7 cut(s)	186, 240, 438, 689, 806, 2054, 2058
AflIII	ACRYGT	1 cut(s)	1136
AhlI	ACTAGT	1 cut(s)	973
AjiI	CACGTC	1 cut(s)	282
AjnI	CCWGG	2 cut(s)	682, 1834
Alw21I	GWGCWC	4 cut(s)	17, 30, 47, 381
Alw26I	GTCTC	2 cut(s)	124, 780
AlwI	GGATC	5 cut(s)	61, 1027, 1456, 1585, 1598
Aor13HI	TCCGGA	1 cut(s)	233
Aor51HI	AGCGCT	1 cut(s)	625
AoxI	GGCC	4 cut(s)	195, 212, 586, 1121
ApeKI	GCWGC	6 cut(s)	286, 853, 941, 1407, 1919, 2083
ApoI	RAATTY	4 cut(s)	104, 253, 262, 1927
AspLEI	GCGC	1 cut(s)	626
AspS9I	GGNCC	4 cut(s)	196, 677, 1121, 1392
AsuC2I	CCSGG	2 cut(s)	200, 273
AsuHPI	GGTGA	7 cut(s)	100, 446, 454, 472, 1079, 2037, 2041
AsuII	TTCGAA	1 cut(s)	562
AvaII	GGWCC	2 cut(s)	677, 1392
BaeGI	GKGCMC	1 cut(s)	1937
BamHI	GGATCC	1 cut(s)	1590
BanI	GGYRCC	1 cut(s)	1934
BanII	GRGCYC	5 cut(s)	17, 47, 278, 381, 1586
BauI	CACGAG	1 cut(s)	362
BbrPI	CACGTG	1 cut(s)	594
BbsI	GAAGAC	2 cut(s)	701, 1106
Bbv12I	GWGCWC	4 cut(s)	17, 30, 47, 381
BbvI	GCAGC	6 cut(s)	298, 865, 928, 1419, 1906, 2070
BccI	CCATC	3 cut(s)	388, 671, 1780
BceAI	ACGGC	2 cut(s)	199, 494
BcgI	CGANNNNNNTGC	2 cut(s)	105, 139
BciT130I	CCWGG	2 cut(s)	684, 1836
BciVI	GTATCC	1 cut(s)	757
BclI	TGATCA	1 cut(s)	736
BcnI	CCSGG	2 cut(s)	200, 273
BcoDI	GTCTC	2 cut(s)	124, 780
BcuI	ACTAGT	1 cut(s)	973
BfaI	CTAG	6 cut(s)	720, 974, 1260, 1373, 1898, 2079
BfmI	CTRYAG	2 cut(s)	114, 1028
BfoI	RGCGCY	1 cut(s)	627
BfuI	GTATCC	1 cut(s)	757
BglII	AGATCT	3 cut(s)	472, 1369, 1415
BisI	GCNGC	8 cut(s)	287, 655, 854, 942, 1344, 1408, 1920, 2084
BlsI	GCNGC	8 cut(s)	288, 656, 855, 943, 1345, 1409, 1921, 2085
BmcAI	AGTACT	1 cut(s)	949
Bme1390I	CCNGG	4 cut(s)	200, 273, 684, 1836
Bme18I	GGWCC	2 cut(s)	677, 1392
BmgBI	CACGTC	1 cut(s)	282
BmgT120I	GGNCC	4 cut(s)	196, 677, 1121, 1392
BmiI	GGNNCC	7 cut(s)	198, 277, 661, 1122, 1592, 1936, 2068
BmrFI	CCNGG	4 cut(s)	200, 273, 684, 1836
BpiI	GAAGAC	2 cut(s)	701, 1106
BpmI	CTGGAG	2 cut(s)	705, 2045
Bpu14I	TTCGAA	1 cut(s)	562
BpuEI	CTTGAG	1 cut(s)	1913
BpuMI	CCSGG	2 cut(s)	200, 273
BsaAI	YACGTR	2 cut(s)	594, 638
BsaBI	GATNNNNATC	1 cut(s)	71
BsaJI	CCNNGG	5 cut(s)	185, 198, 1586, 1647, 1949
BsaWI	WCCGGW	5 cut(s)	233, 431, 546, 567, 2009
BsaXI	ACNNNNNCTCC	2 cut(s)	1833, 1863
Bsc4I	CCNNNNNNNGG	7 cut(s)	186, 240, 438, 689, 806, 2054, 2058
Bse118I	RCCGGY	4 cut(s)	157, 457, 1438, 2051
Bse1I	ACTGG	5 cut(s)	945, 1215, 1563, 1847, 2070
Bse3DI	GCAATG	1 cut(s)	26
Bse8I	GATNNNNATC	1 cut(s)	71
BseAI	TCCGGA	1 cut(s)	233
BseBI	CCWGG	2 cut(s)	684, 1836
BseDI	CCNNGG	5 cut(s)	185, 198, 1586, 1647, 1949
BseGI	GGATG	4 cut(s)	345, 834, 1559, 1680
BseJI	GATNNNNATC	1 cut(s)	71
BseLI	CCNNNNNNNGG	7 cut(s)	186, 240, 438, 689, 806, 2054, 2058
BseMI	GCAATG	1 cut(s)	26
BseMII	CTCAG	1 cut(s)	618
BseNI	ACTGG	5 cut(s)	945, 1215, 1563, 1847, 2070
BseRI	GAGGAG	6 cut(s)	358, 380, 421, 978, 1444, 1814
BseSI	GKGCMC	1 cut(s)	1937
BseXI	GCAGC	6 cut(s)	298, 865, 928, 1419, 1906, 2070
BseYI	CCCAGC	1 cut(s)	307
Bsh1236I	CGCG	1 cut(s)	144
BshFI	GGCC	4 cut(s)	197, 214, 588, 1123
BshNI	GGYRCC	1 cut(s)	1934
BsiHKAI	GWGCWC	4 cut(s)	17, 30, 47, 381
BslFI	GGGAC	1 cut(s)	618
BslI	CCNNNNNNNGG	7 cut(s)	186, 240, 438, 689, 806, 2054, 2058
BsmAI	GTCTC	2 cut(s)	124, 780
BsmBI	CGTCTC	1 cut(s)	780
BsmFI	GGGAC	1 cut(s)	618
BsmI	GAATGC	1 cut(s)	729
BsnI	GGCC	4 cut(s)	197, 214, 588, 1123
Bsp119I	TTCGAA	1 cut(s)	562
Bsp1286I	GDGCHC	7 cut(s)	17, 30, 47, 278, 381, 1586, 1937
Bsp13I	TCCGGA	1 cut(s)	233
Bsp1407I	TGTACA	1 cut(s)	354
Bsp19I	CCATGG	3 cut(s)	185, 1586, 1647
BspACI	CCGC	6 cut(s)	144, 654, 657, 1344, 1594, 2055
BspANI	GGCC	4 cut(s)	197, 214, 588, 1123
BspCNI	CTCAG	1 cut(s)	619
BspEI	TCCGGA	1 cut(s)	233
BspFNI	CGCG	1 cut(s)	144
BspHI	TCATGA	2 cut(s)	466, 1632
BspLI	GGNNCC	7 cut(s)	198, 277, 661, 1122, 1592, 1936, 2068
BspMAI	CTGCAG	1 cut(s)	118
BspPI	GGATC	5 cut(s)	61, 1027, 1456, 1585, 1598
BspT104I	TTCGAA	1 cut(s)	562
BspT107I	GGYRCC	1 cut(s)	1934
BsrDI	GCAATG	1 cut(s)	26
BsrFI	RCCGGY	4 cut(s)	157, 457, 1438, 2051
BsrGI	TGTACA	1 cut(s)	354
BsrI	ACTGG	5 cut(s)	945, 1215, 1563, 1847, 2070
BssAI	RCCGGY	4 cut(s)	157, 457, 1438, 2051
BssECI	CCNNGG	5 cut(s)	185, 198, 1586, 1647, 1949
BssSI	CACGAG	1 cut(s)	362
BssT1I	CCWWGG	3 cut(s)	185, 1586, 1647
Bst2BI	CACGAG	1 cut(s)	362
Bst2UI	CCWGG	2 cut(s)	684, 1836
Bst4CI	ACNGT	3 cut(s)	521, 1198, 1392
Bst6I	CTCTTC	3 cut(s)	127, 1421, 1776
BstAPI	GCANNNNNTGC	2 cut(s)	25, 941
BstAUI	TGTACA	1 cut(s)	354
BstBAI	YACGTR	2 cut(s)	594, 638
BstBI	TTCGAA	1 cut(s)	562
BstC8I	GCNNGC	6 cut(s)	17, 26, 377, 483, 729, 1917
BstDEI	CTNAG	1 cut(s)	627
BstDSI	CCRYGG	3 cut(s)	185, 1586, 1647
BstENI	CCTNNNNNAGG	1 cut(s)	804
BstF5I	GGATG	4 cut(s)	345, 834, 1559, 1680
BstFNI	CGCG	1 cut(s)	144
BstH2I	RGCGCY	1 cut(s)	627
BstHHI	GCGC	1 cut(s)	626
BstMAI	GTCTC	2 cut(s)	124, 780
BstMWI	GCNNNNNNNGC	7 cut(s)	21, 25, 358, 594, 941, 1211, 1852
BstNI	CCWGG	2 cut(s)	684, 1836
BstNSI	RCATGY	1 cut(s)	1140
BstSCI	CCNGG	4 cut(s)	198, 271, 682, 1834
BstSFI	CTRYAG	2 cut(s)	114, 1028
BstSLI	GKGCMC	1 cut(s)	1937
BstUI	CGCG	1 cut(s)	144
BstV1I	GCAGC	6 cut(s)	298, 865, 928, 1419, 1906, 2070
BstV2I	GAAGAC	2 cut(s)	701, 1106
BstX2I	RGATCY	7 cut(s)	66, 472, 1032, 1369, 1415, 1461, 1590
BstXI	CCANNNNNNTGG	1 cut(s)	977
BstYI	RGATCY	7 cut(s)	66, 472, 1032, 1369, 1415, 1461, 1590
BsuI	GTATCC	1 cut(s)	757
BsuRI	GGCC	4 cut(s)	197, 214, 588, 1123
BtgI	CCRYGG	3 cut(s)	185, 1586, 1647
BtrI	CACGTC	1 cut(s)	282
BtsCI	GGATG	4 cut(s)	345, 834, 1559, 1680
BtsI	GCAGTG	2 cut(s)	882, 1603
BtsIMutI	CAGTG	4 cut(s)	882, 1603, 1869, 2077
Cac8I	GCNNGC	6 cut(s)	17, 26, 377, 483, 729, 1917
CciI	TCATGA	2 cut(s)	466, 1632
CfoI	GCGC	1 cut(s)	626
Cfr10I	RCCGGY	4 cut(s)	157, 457, 1438, 2051
Cfr13I	GGNCC	4 cut(s)	196, 677, 1121, 1392
CseI	GACGC	1 cut(s)	1875
Csp6I	GTAC	4 cut(s)	355, 948, 1094, 1822
CviAII	CATG	9 cut(s)	48, 186, 467, 989, 1137, 1244, 1587, 1633, 1648
CviQI	GTAC	4 cut(s)	355, 948, 1094, 1822
DdeI	CTNAG	1 cut(s)	627
DraIII	CACNNNGTG	1 cut(s)	1067
Eam1104I	CTCTTC	3 cut(s)	127, 1421, 1776
EarI	CTCTTC	3 cut(s)	127, 1421, 1776
EciI	GGCGGA	1 cut(s)	2070
Ecl136II	GAGCTC	3 cut(s)	15, 45, 379
Eco130I	CCWWGG	3 cut(s)	185, 1586, 1647
Eco24I	GRGCYC	5 cut(s)	17, 47, 278, 381, 1586
Eco32I	GATATC	1 cut(s)	1534
Eco47I	GGWCC	2 cut(s)	677, 1392
Eco47III	AGCGCT	1 cut(s)	625
Eco53kI	GAGCTC	3 cut(s)	15, 45, 379
Eco57I	CTGAAG	1 cut(s)	77
Eco72I	CACGTG	1 cut(s)	594
EcoICRI	GAGCTC	3 cut(s)	15, 45, 379
EcoNI	CCTNNNNNAGG	1 cut(s)	804
EcoO109I	RGGNCCY	1 cut(s)	1121
EcoRII	CCWGG	2 cut(s)	682, 1834
EcoRV	GATATC	1 cut(s)	1534
EcoT14I	CCWWGG	3 cut(s)	185, 1586, 1647
EcoT22I	ATGCAT	2 cut(s)	1456, 1795
EcoT38I	GRGCYC	5 cut(s)	17, 47, 278, 381, 1586
ErhI	CCWWGG	3 cut(s)	185, 1586, 1647
Esp3I	CGTCTC	1 cut(s)	780
FaeI	CATG	9 cut(s)	51, 189, 470, 992, 1140, 1247, 1590, 1636, 1651
FalI	AAGNNNNNCTT	4 cut(s)	686, 718, 1461, 1493
FaqI	GGGAC	1 cut(s)	618
FatI	CATG	9 cut(s)	47, 185, 466, 988, 1136, 1243, 1586, 1632, 1647
FauNDI	CATATG	1 cut(s)	888
FbaI	TGATCA	1 cut(s)	736
Fnu4HI	GCNGC	8 cut(s)	287, 655, 854, 942, 1344, 1408, 1920, 2084
FokI	GGATG	4 cut(s)	352, 841, 1546, 1687
FriOI	GRGCYC	5 cut(s)	17, 47, 278, 381, 1586
Fsp4HI	GCNGC	8 cut(s)	287, 655, 854, 942, 1344, 1408, 1920, 2084
FspBI	CTAG	6 cut(s)	720, 974, 1260, 1373, 1898, 2079
GlaI	GCGC	1 cut(s)	625
GluI	GCNGC	8 cut(s)	287, 655, 854, 942, 1344, 1408, 1920, 2084
GsaI	CCCAGC	1 cut(s)	311
GsuI	CTGGAG	2 cut(s)	705, 2045
HaeII	RGCGCY	1 cut(s)	627
HaeIII	GGCC	4 cut(s)	197, 214, 588, 1123
HgaI	GACGC	1 cut(s)	1875
HhaI	GCGC	1 cut(s)	626
Hin1II	CATG	9 cut(s)	51, 189, 470, 992, 1140, 1247, 1590, 1636, 1651
Hin6I	GCGC	1 cut(s)	624
HinP1I	GCGC	1 cut(s)	624
HindIII	AAGCTT	1 cut(s)	1989
HphI	GGTGA	7 cut(s)	100, 446, 454, 472, 1079, 2037, 2041
Hpy99I	CGWCG	2 cut(s)	138, 286
HpyAV	CCTTC	7 cut(s)	101, 438, 487, 1032, 1080, 1084, 1110
HpyCH4III	ACNGT	3 cut(s)	521, 1198, 1392
HpyCH4IV	ACGT	4 cut(s)	281, 593, 637, 1008
HpyF10VI	GCNNNNNNNGC	7 cut(s)	21, 25, 358, 594, 941, 1211, 1852
HpyF3I	CTNAG	1 cut(s)	627
HpySE526I	ACGT	4 cut(s)	281, 593, 637, 1008
Hsp92II	CATG	9 cut(s)	51, 189, 470, 992, 1140, 1247, 1590, 1636, 1651
HspAI	GCGC	1 cut(s)	624
Kpn2I	TCCGGA	1 cut(s)	233
Ksp22I	TGATCA	1 cut(s)	736
LmnI	GCTCC	2 cut(s)	281, 304
Lsp1109I	GCAGC	6 cut(s)	298, 865, 928, 1419, 1906, 2070
MaeI	CTAG	6 cut(s)	720, 974, 1260, 1373, 1898, 2079
MaeII	ACGT	4 cut(s)	281, 593, 637, 1008
MaeIII	GTNAC	5 cut(s)	315, 638, 1057, 1522, 1600
MflI	RGATCY	7 cut(s)	66, 472, 1032, 1369, 1415, 1461, 1590
MhlI	GDGCHC	7 cut(s)	17, 30, 47, 278, 381, 1586, 1937
MlyI	GAGTC	3 cut(s)	418, 975, 1364
MmeI	TCCRAC	2 cut(s)	958, 994
Mph1103I	ATGCAT	2 cut(s)	1456, 1795
MroI	TCCGGA	1 cut(s)	233
MseI	TTAA	5 cut(s)	531, 704, 1976, 2004, 2034
MslI	CAYNNNNRTG	3 cut(s)	1135, 1248, 1940
MspA1I	CMGCKG	3 cut(s)	1346, 1544, 1915
MspR9I	CCNGG	4 cut(s)	200, 273, 684, 1836
Mva1269I	GAATGC	1 cut(s)	729
MvaI	CCWGG	2 cut(s)	684, 1836
MvnI	CGCG	1 cut(s)	144
MwoI	GCNNNNNNNGC	7 cut(s)	21, 25, 358, 594, 941, 1211, 1852
NciI	CCSGG	2 cut(s)	200, 273
NcoI	CCATGG	3 cut(s)	185, 1586, 1647
NdeI	CATATG	1 cut(s)	888
NlaIII	CATG	9 cut(s)	51, 189, 470, 992, 1140, 1247, 1590, 1636, 1651
NlaIV	GGNNCC	7 cut(s)	198, 277, 661, 1122, 1592, 1936, 2068
NmeAIII	GCCGAG	1 cut(s)	623
NmuCI	GTSAC	2 cut(s)	638, 1057
NsiI	ATGCAT	2 cut(s)	1456, 1795
NspI	RCATGY	1 cut(s)	1140
NspV	TTCGAA	1 cut(s)	562
PagI	TCATGA	2 cut(s)	466, 1632
PciI	ACATGT	1 cut(s)	1136
PcsI	WCGNNNNNNNCGW	1 cut(s)	148
PctI	GAATGC	1 cut(s)	729
PfeI	GAWTC	9 cut(s)	190, 205, 268, 463, 1678, 1691, 1831, 1945, 1960
PflMI	CCANNNNNTGG	1 cut(s)	186
PfoI	TCCNGGA	1 cut(s)	682
PkrI	GCNGC	8 cut(s)	288, 656, 855, 943, 1345, 1409, 1921, 2085
PleI	GAGTC	3 cut(s)	417, 974, 1363
PmaCI	CACGTG	1 cut(s)	594
PmlI	CACGTG	1 cut(s)	594
PpsI	GAGTC	3 cut(s)	417, 974, 1363
Ppu21I	YACGTR	2 cut(s)	594, 638
PscI	ACATGT	1 cut(s)	1136
Psp124BI	GAGCTC	3 cut(s)	17, 47, 381
Psp6I	CCWGG	2 cut(s)	682, 1834
PspCI	CACGTG	1 cut(s)	594
PspFI	CCCAGC	1 cut(s)	307
PspGI	CCWGG	2 cut(s)	682, 1834
PspN4I	GGNNCC	7 cut(s)	198, 277, 661, 1122, 1592, 1936, 2068
PspPI	GGNCC	4 cut(s)	196, 677, 1121, 1392
PstI	CTGCAG	1 cut(s)	118
PsuI	RGATCY	7 cut(s)	66, 472, 1032, 1369, 1415, 1461, 1590
PvuII	CAGCTG	2 cut(s)	1544, 1915
RsaI	GTAC	4 cut(s)	356, 949, 1095, 1823
RsaNI	GTAC	4 cut(s)	355, 948, 1094, 1822
RseI	CAYNNNNRTG	3 cut(s)	1135, 1248, 1940
SacI	GAGCTC	3 cut(s)	17, 47, 381
SaqAI	TTAA	5 cut(s)	531, 704, 1976, 2004, 2034
SatI	GCNGC	8 cut(s)	287, 655, 854, 942, 1344, 1408, 1920, 2084
Sau96I	GGNCC	4 cut(s)	196, 677, 1121, 1392
ScaI	AGTACT	1 cut(s)	949
SchI	GAGTC	3 cut(s)	418, 975, 1364
ScrFI	CCNGG	4 cut(s)	200, 273, 684, 1836
SduI	GDGCHC	7 cut(s)	17, 30, 47, 278, 381, 1586, 1937
SfcI	CTRYAG	2 cut(s)	114, 1028
SfuI	TTCGAA	1 cut(s)	562
SinI	GGWCC	2 cut(s)	677, 1392
SmiMI	CAYNNNNRTG	3 cut(s)	1135, 1248, 1940
SmlI	CTYRAG	1 cut(s)	1892
SmoI	CTYRAG	1 cut(s)	1892
SpeI	ACTAGT	1 cut(s)	973
SsiI	CCGC	6 cut(s)	144, 654, 657, 1344, 1594, 2055
SspMI	CTAG	6 cut(s)	720, 974, 1260, 1373, 1898, 2079
SstI	GAGCTC	3 cut(s)	17, 47, 381
StyD4I	CCNGG	4 cut(s)	198, 271, 682, 1834
StyI	CCWWGG	3 cut(s)	185, 1586, 1647
TaaI	ACNGT	3 cut(s)	521, 1198, 1392
TaiI	ACGT	4 cut(s)	284, 596, 640, 1011
TaqI	TCGA	7 cut(s)	77, 136, 491, 562, 1734, 1807, 1851
TatI	WGTACW	3 cut(s)	354, 947, 1821
TauI	GCSGC	2 cut(s)	657, 1346
TfiI	GAWTC	9 cut(s)	190, 205, 268, 463, 1678, 1691, 1831, 1945, 1960
Tru1I	TTAA	5 cut(s)	531, 704, 1976, 2004, 2034
Tru9I	TTAA	5 cut(s)	531, 704, 1976, 2004, 2034
TscAI	CASTG	4 cut(s)	882, 1603, 1876, 2077
TseFI	GTSAC	2 cut(s)	638, 1057
TseI	GCWGC	6 cut(s)	286, 853, 941, 1407, 1919, 2083
Tsp45I	GTSAC	2 cut(s)	638, 1057
TspDTI	ATGAA	9 cut(s)	483, 927, 1028, 1086, 1527, 1584, 1589, 1649, 1958
TspGWI	ACGGA	2 cut(s)	804, 1229
TspRI	CASTG	4 cut(s)	882, 1603, 1876, 2077
Van91I	CCANNNNNTGG	1 cut(s)	186
VpaK11BI	GGWCC	2 cut(s)	677, 1392
XagI	CCTNNNNNAGG	1 cut(s)	804
XapI	RAATTY	4 cut(s)	104, 253, 262, 1927
XbaI	TCTAGA	1 cut(s)	1372
XceI	RCATGY	1 cut(s)	1140
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1654
XspI	CTAG	6 cut(s)	720, 974, 1260, 1373, 1898, 2079
ZrmI	AGTACT	1 cut(s)	949
Zsp2I	ATGCAT	2 cut(s)	1456, 1795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.