pycom01g12830

ATPase family associated with various cellular activities (AAA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008253
Physical Location & Seq
Reverse (-)
13482586 .. 13484427
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g12830.2

Sequence Viewer

Length: 1110 bp
ATGACGATAGACAACGTAATGAAAAATCCTTCTCTACAGATCCTTGTCGGTGGCATAGAGAGTGTGACACTTGGTGATGAAGAAGCAAGAAGAAGGAAGGTACAGAAGACAATTCTGGAAAGAAGGGGACCTCCTACATTTACATGTGCTGTGGAGATTATATCTAAAACAGAACTTCGTGTGCATCACAGATTAGATGCAACTGTTGATGCTATACTGGCTGGAAAACCTCCTTTGTTTGAAATCCGTCGTGTTGATGCAGATGCTGGTGGCTCTCGCAAGTCTATTTCAATACCTGAAAAGAGCTATCTACAAGTGTCTGATTTTACTGTTAAAAAGAACATTGATGCCGCTGATATAGAGTCTGACAAGGAAGATCTAGACCATTCCCCTACACGGTCCAAAAAATTGAGCAGCAAAAGATCTGTGAAGAAGAGGAGTTTGCCGGTTTGTGTATATGCATACAAGATCCTTGAAGCCGATCTTCTACAAGTAGCAAAGGTAATGGGGCTTGAAGATGAAATAGAAGTGACTGATGATATCGGAACAGCTGATGTAATAATAGCATCCAGTTATGAAATGAAACAGAACCCATGGATCCGCAGTGTAACAAAGTTCCACGACTTGCCTGTATTTGTCATGAAGTCAAATACCATGGCACAAATGGTCAAGGCAGTTCGGATGATTCTTGAAATGGAATCTGTTGGCTCTTTGCGAAAGCAGTTAGTCAATAGTTCTTTCGACATTGAAATTGAAGATGATGCCCCGAAAAGAAAACCATCTTTGGAAGAGATTGATGCATTGGAGGAGGTTCGACTTGCGATTGAGTACATCGTGATTCCTGGTGGAGAGCCAGTAGAGCTTCTCCCAAGATGCTCTGAAATAATCGCCCGACAGCTTGAGCTAGTGGAAAGTTATCAGCTGGCTGCTGAAAATTCAGGCACCGAGATGAATCCAAGGTTGCAGATTCTTCCGATGAGATTAAACAAAAAGAAAGCTTCAAAACCTGTTAAGTCCGGTTCAAGTTTTCTGGAGGTGATTAACTCTAAATCACTAACCGGCGGAGGTGGAGGAACCAGTGTTACTAGGCTGCCTATTATATCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

370

Amino Acids

40.93

Weight (kDa)

6.23

Isoelectric Point (pI)

59.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 941
AciI CCGC 3 cut(s) 351, 601, 1062
AclWI GGATC 4 cut(s) 34, 463, 592, 605
AcsI RAATTY 1 cut(s) 934
AdeI CACNNNGTG 1 cut(s) 74
AfaI GTAC 2 cut(s) 102, 830
AfiI CCNNNNNNNGG 1 cut(s) 396
AflIII ACRYGT 1 cut(s) 143
AgsI TTSAA 9 cut(s) 242, 291, 476, 515, 692, 749, 755, 1002, 1023
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 7 cut(s) 306, 551, 862, 898, 904, 922, 998
AluI AGCT 7 cut(s) 306, 551, 862, 898, 904, 922, 998
AlwI GGATC 4 cut(s) 34, 463, 592, 605
AlwNI CAGNNNCTG 1 cut(s) 266
ApeKI GCWGC 3 cut(s) 414, 926, 1090
ApoI RAATTY 1 cut(s) 934
AspS9I GGNCC 2 cut(s) 128, 399
AsuHPI GGTGA 2 cut(s) 86, 1048
AvaII GGWCC 2 cut(s) 128, 399
BamHI GGATCC 1 cut(s) 597
BanI GGYRCC 1 cut(s) 941
BbsI GAAGAC 1 cut(s) 113
BbvI GCAGC 3 cut(s) 426, 913, 1077
BccI CCATC 1 cut(s) 787
BciT130I CCWGG 1 cut(s) 843
BfaI CTAG 3 cut(s) 380, 905, 1086
BfmI CTRYAG 1 cut(s) 35
BglII AGATCT 2 cut(s) 376, 422
BisI GCNGC 4 cut(s) 351, 415, 927, 1091
BlsI GCNGC 4 cut(s) 352, 416, 928, 1092
Bme1390I CCNGG 1 cut(s) 843
Bme18I GGWCC 2 cut(s) 128, 399
BmgT120I GGNCC 2 cut(s) 128, 399
BmiI GGNNCC 4 cut(s) 129, 599, 943, 1075
BmrFI CCNGG 1 cut(s) 843
BpiI GAAGAC 1 cut(s) 113
BpmI CTGGAG 1 cut(s) 1052
BpuEI CTTGAG 1 cut(s) 920
BsaJI CCNNGG 3 cut(s) 593, 654, 956
BsaWI WCCGGW 1 cut(s) 1016
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse118I RCCGGY 2 cut(s) 445, 1058
Bse1I ACTGG 4 cut(s) 222, 570, 854, 1077
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 3 cut(s) 593, 654, 956
BseGI GGATG 2 cut(s) 566, 687
BseLI CCNNNNNNNGG 1 cut(s) 396
BseNI ACTGG 4 cut(s) 222, 570, 854, 1077
BseRI GAGGAG 2 cut(s) 451, 821
BseXI GCAGC 3 cut(s) 426, 913, 1077
BshNI GGYRCC 1 cut(s) 941
BsiSI CCGG 3 cut(s) 446, 1017, 1059
BslFI GGGAC 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 396
BsmFI GGGAC 1 cut(s) 141
Bsp143I GATC 6 cut(s) 39, 376, 422, 468, 481, 597
Bsp19I CCATGG 2 cut(s) 593, 654
BspACI CCGC 3 cut(s) 351, 601, 1062
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 4 cut(s) 129, 599, 943, 1075
BspPI GGATC 4 cut(s) 34, 463, 592, 605
BspT107I GGYRCC 1 cut(s) 941
BsrFI RCCGGY 2 cut(s) 445, 1058
BsrI ACTGG 4 cut(s) 222, 570, 854, 1077
BssAI RCCGGY 2 cut(s) 445, 1058
BssECI CCNNGG 3 cut(s) 593, 654, 956
BssMI GATC 6 cut(s) 39, 376, 422, 468, 481, 597
BssT1I CCWWGG 3 cut(s) 593, 654, 956
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 205, 331, 399
Bst6I CTCTTC 2 cut(s) 428, 783
BstC8I GCNNGC 1 cut(s) 924
BstDSI CCRYGG 2 cut(s) 593, 654
BstF5I GGATG 2 cut(s) 566, 687
BstKTI GATC 6 cut(s) 42, 379, 425, 471, 484, 600
BstMBI GATC 6 cut(s) 39, 376, 422, 468, 481, 597
BstMWI GCNNNNNNNGC 2 cut(s) 218, 859
BstNI CCWGG 1 cut(s) 843
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 841
BstSFI CTRYAG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 426, 913, 1077
BstV2I GAAGAC 1 cut(s) 113
BstX2I RGATCY 5 cut(s) 39, 376, 422, 468, 597
BstYI RGATCY 5 cut(s) 39, 376, 422, 468, 597
BtgI CCRYGG 2 cut(s) 593, 654
BtsCI GGATG 2 cut(s) 566, 687
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 2 cut(s) 610, 1084
Cac8I GCNNGC 1 cut(s) 924
CaiI CAGNNNCTG 1 cut(s) 266
CciI TCATGA 1 cut(s) 639
Cfr10I RCCGGY 2 cut(s) 445, 1058
Cfr13I GGNCC 2 cut(s) 128, 399
Csp6I GTAC 2 cut(s) 101, 829
CviAII CATG 4 cut(s) 144, 594, 640, 655
CviQI GTAC 2 cut(s) 101, 829
DpnI GATC 6 cut(s) 41, 378, 424, 470, 483, 599
DpnII GATC 6 cut(s) 39, 376, 422, 468, 481, 597
DraIII CACNNNGTG 1 cut(s) 74
Eam1104I CTCTTC 2 cut(s) 428, 783
EarI CTCTTC 2 cut(s) 428, 783
EciI GGCGGA 1 cut(s) 1077
Eco130I CCWWGG 3 cut(s) 593, 654, 956
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 2 cut(s) 128, 399
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 841
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 3 cut(s) 593, 654, 956
EcoT22I ATGCAT 2 cut(s) 463, 802
ErhI CCWWGG 3 cut(s) 593, 654, 956
FaeI CATG 4 cut(s) 147, 597, 643, 658
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FaqI GGGAC 1 cut(s) 141
FatI CATG 4 cut(s) 143, 593, 639, 654
Fnu4HI GCNGC 4 cut(s) 351, 415, 927, 1091
FokI GGATG 2 cut(s) 553, 694
Fsp4HI GCNGC 4 cut(s) 351, 415, 927, 1091
FspBI CTAG 3 cut(s) 380, 905, 1086
GluI GCNGC 4 cut(s) 351, 415, 927, 1091
GsuI CTGGAG 1 cut(s) 1052
HapII CCGG 3 cut(s) 446, 1017, 1059
Hin1II CATG 4 cut(s) 147, 597, 643, 658
HindIII AAGCTT 1 cut(s) 996
HinfI GANTC 6 cut(s) 362, 685, 698, 838, 952, 967
HpaII CCGG 3 cut(s) 446, 1017, 1059
HphI GGTGA 2 cut(s) 86, 1048
Hpy188I TCNGA 7 cut(s) 322, 367, 545, 681, 880, 975, 1105
Hpy188III TCNNGA 6 cut(s) 116, 380, 640, 689, 835, 1031
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 4 cut(s) 39, 87, 91, 117
HpyCH4III ACNGT 3 cut(s) 205, 331, 399
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 6 cut(s) 184, 200, 260, 461, 800, 964
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 859
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 4 cut(s) 147, 597, 643, 658
Kzo9I GATC 6 cut(s) 39, 376, 422, 468, 481, 597
Lsp1109I GCAGC 3 cut(s) 426, 913, 1077
MaeI CTAG 3 cut(s) 380, 905, 1086
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 4 cut(s) 64, 529, 607, 1081
MalI GATC 6 cut(s) 41, 378, 424, 470, 483, 599
MboI GATC 6 cut(s) 39, 376, 422, 468, 481, 597
MflI RGATCY 5 cut(s) 39, 376, 422, 468, 597
MluCI AATT 4 cut(s) 111, 407, 750, 934
MlyI GAGTC 1 cut(s) 371
MnlI CCTC 8 cut(s) 141, 240, 429, 799, 802, 1027, 1058, 1064
Mph1103I ATGCAT 2 cut(s) 463, 802
MseI TTAA 4 cut(s) 333, 983, 1011, 1041
MslI CAYNNNNRTG 2 cut(s) 142, 947
MspA1I CMGCKG 3 cut(s) 353, 551, 922
MspI CCGG 3 cut(s) 446, 1017, 1059
MspR9I CCNGG 1 cut(s) 843
MvaI CCWGG 1 cut(s) 843
MwoI GCNNNNNNNGC 2 cut(s) 218, 859
NcoI CCATGG 2 cut(s) 593, 654
NdeII GATC 6 cut(s) 39, 376, 422, 468, 481, 597
NlaIII CATG 4 cut(s) 147, 597, 643, 658
NlaIV GGNNCC 4 cut(s) 129, 599, 943, 1075
NmuCI GTSAC 2 cut(s) 64, 529
NsiI ATGCAT 2 cut(s) 463, 802
NspI RCATGY 1 cut(s) 147
PagI TCATGA 1 cut(s) 639
PciI ACATGT 1 cut(s) 143
PfeI GAWTC 5 cut(s) 685, 698, 838, 952, 967
PkrI GCNGC 4 cut(s) 352, 416, 928, 1092
PleI GAGTC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 370
PpuMI RGGWCCY 1 cut(s) 128
PscI ACATGT 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 4 cut(s) 129, 599, 943, 1075
PspPI GGNCC 2 cut(s) 128, 399
PspPPI RGGWCCY 1 cut(s) 128
PsrI GAACNNNNNNTAC 2 cut(s) 1066, 1098
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 5 cut(s) 39, 376, 422, 468, 597
PvuII CAGCTG 2 cut(s) 551, 922
RsaI GTAC 2 cut(s) 102, 830
RsaNI GTAC 2 cut(s) 101, 829
RseI CAYNNNNRTG 2 cut(s) 142, 947
SaqAI TTAA 4 cut(s) 333, 983, 1011, 1041
SatI GCNGC 4 cut(s) 351, 415, 927, 1091
Sau3AI GATC 6 cut(s) 39, 376, 422, 468, 481, 597
Sau96I GGNCC 2 cut(s) 128, 399
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 1 cut(s) 843
SfcI CTRYAG 1 cut(s) 35
SinI GGWCC 2 cut(s) 128, 399
SmiMI CAYNNNNRTG 2 cut(s) 142, 947
SmlI CTYRAG 1 cut(s) 899
SmoI CTYRAG 1 cut(s) 899
Sse9I AATT 4 cut(s) 111, 407, 750, 934
SsiI CCGC 3 cut(s) 351, 601, 1062
SspMI CTAG 3 cut(s) 380, 905, 1086
StyD4I CCNGG 1 cut(s) 841
StyI CCWWGG 3 cut(s) 593, 654, 956
TaaI ACNGT 3 cut(s) 205, 331, 399
TaiI ACGT 1 cut(s) 18
TaqI TCGA 2 cut(s) 741, 814
TasI AATT 4 cut(s) 111, 407, 750, 934
TatI WGTACW 1 cut(s) 828
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 5 cut(s) 685, 698, 838, 952, 967
Tru1I TTAA 4 cut(s) 333, 983, 1011, 1041
Tru9I TTAA 4 cut(s) 333, 983, 1011, 1041
TscAI CASTG 2 cut(s) 610, 1084
TseFI GTSAC 2 cut(s) 64, 529
TseI GCWGC 3 cut(s) 414, 926, 1090
Tsp45I GTSAC 2 cut(s) 64, 529
TspDTI ATGAA 7 cut(s) 35, 93, 534, 591, 596, 656, 965
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 2 cut(s) 610, 1084
VpaK11BI GGWCC 2 cut(s) 128, 399
XapI RAATTY 1 cut(s) 934
XbaI TCTAGA 1 cut(s) 379
XceI RCATGY 1 cut(s) 147
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
XspI CTAG 3 cut(s) 380, 905, 1086
Zsp2I ATGCAT 2 cut(s) 463, 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.