Rroxscaffold_4G00294260

ATPase family associated with various cellular activities (AAA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008253
Physical Location & Seq
Forward (+)
14391698 .. 14395439
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294260.1

Sequence Viewer

Length: 1566 bp
ATGAAGAGCGAGCTGTACAGGCATGAGGAGATCGGCAGGCTCATTGAGGTCGTCATGGATTTGGGGCGGCCTCCGATTGCCAGGTTTCCCACCGGCGATTGGGTCATCTCGGAATATCCCGTCAGCCAAGAAGATCTCCGGCTCGCTGTATCCAAGGTTAGTGAGTTTTCAGATGACAACCGTTCTGGTATTAATAACTCATTACACCGGATAAGTGCTATCCGAAACCGAAAGCTGCAAATCATTGGACTTACTTGCCGGGTGGGGAGAGCTGTGTCGGGAAGTGCTGAGATCATCCGCGACTTGGTTGAGGGTGGTGGTTCAATCTTGGTTATAGGACCTCCTGGAGTTGGCAAGACAACCTTGATCAGAGAAATTGCTAGAATGTTGGCGGATGAGCATATGAAGCGTGTTGTCATTGTGGATACATCGAATGAGATTGGGGGAGATGGAGATGTACCTCATGCGGGAATAGGCCGTGCAAGGAGGATGCAAGTTCCTAACGTGAACATGCAGCATAATGTTATGATTGAAGCAGTTGAAAATCATATGCCTCAAACCATTATAATAGATGAAATTGGGACAGAGCTTGAAGCATTGGCTTCCAGTACCATTGCTCAAAGAGGAGTTCAGCTAGTTGGAACAGCACATGGAATGACGATAGACAACATAATAAAGAATCCTTCTTTACAAATCCTTGTTGGTGGCATAGAGAGTGTGACCCTGGGAGATGAAGAAGCAAGAAAGAGAAAAGTTCAAAAGACAATTCTTGAAAGAAAGGGGCCTCCTACATTTACATGTGCTGTTGAGATTATATCTAAAACAGAGTTTCGTGTTCATCACAGATTAGATGCAACCGTGGATGCTATACTAGCTGGAAAATCTGCTCTGTTTGAAGTTCGTCGTACTGATGCGGATGCTGATGACTCTTGCATGTCTACTCAAATATCTGAACCGAACTATCAAAAAGTATCAGATTTTGGTGTTACTAAAGACATTATTGCTAGTGTTTTGGAGTCTGACGAGGAAGATCTAGATCCTTCACCCAATGTGTCTAGAAGATTGAGCAGTGATAAATCTGTGAAGAAGAGAAGTTCACCAGTGTGTATATATACATACAAGATCCTTGAAGCTGACCTACTGCAAGTAGCACAGGTGATGGGGCTTGAGGATGAAATTGAGGTAACTGATGATATTGGAACAGCAGATGCAATTATGGCATCCAGTTCTGAGATGAAGCAAAACCCATGGATTCGTGGTGTAGCAAAATTCCACCAGTTGCCTGTATTTGTCATGAAGTCAAATACCATGGCACAAATCGTGAAGGCAGTTCGTACGATTCTTGAAATGGAATCAGTTGGTTCTCTGCCAAAGCAGCTTGTCAATAATTCCTTTGACATTGAAATTGAAGATGATGCACCCAAAAGAAAACCATCTTTAGAAGAGATCGATGCATTGGAGGTGCATTTCCTTTCCCATCCCACCTATTTTCTATGGATACCTCTACCGGCAGTTTCTGAGAAATTGTCCACTCTGTTGAGGTTCGACTTGCAATCGAGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

521

Amino Acids

57.51

Weight (kDa)

5.29

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spore_III_AA PF19568 81 - 234 2.8e-13 Sporulation stage III, protein AA
AAA_16 PF13191 102 - 137 8.3e-06 AAA ATPase domain
AAA PF00004 109 - 194 5.2e-06 ATPase family associated with various cellular activities (AAA)
PTPase PF25516 368 - 448 1.6e-28 Phosphotyrosine protein phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AccI GTMKAC 1 cut(s) 938
AccII CGCG 1 cut(s) 300
AciI CCGC 5 cut(s) 67, 298, 392, 467, 914
AclWI GGATC 2 cut(s) 1031, 1117
AcsI RAATTY 1 cut(s) 1268
AfaI GTAC 5 cut(s) 17, 459, 610, 907, 1336
AfiI CCNNNNNNNGG 4 cut(s) 99, 304, 350, 467
AflIII ACRYGT 1 cut(s) 797
AjnI CCWGG 3 cut(s) 80, 343, 723
AleI CACNNNNGTG 1 cut(s) 1102
AluBI AGCT 8 cut(s) 13, 235, 272, 589, 634, 875, 1133, 1378
AluI AGCT 8 cut(s) 13, 235, 272, 589, 634, 875, 1133, 1378
AlwI GGATC 2 cut(s) 1031, 1117
AlwNI CAGNNNCTG 1 cut(s) 1517
AoxI GGCC 3 cut(s) 68, 475, 782
ApeKI GCWGC 3 cut(s) 235, 514, 1375
ApoI RAATTY 1 cut(s) 1268
AseI ATTAAT 1 cut(s) 192
AspS9I GGNCC 2 cut(s) 338, 782
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 3 cut(s) 1035, 1089, 1168
AvaII GGWCC 1 cut(s) 338
BarI GAAGNNNNNNTAC 2 cut(s) 1122, 1154
BbvI GCAGC 3 cut(s) 222, 526, 1387
BccI CCATC 4 cut(s) 443, 1153, 1441, 1485
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 3 cut(s) 82, 345, 725
BciVI GTATCC 3 cut(s) 160, 418, 1491
BclI TGATCA 1 cut(s) 366
BcnI CCSGG 1 cut(s) 260
BfaI CTAG 6 cut(s) 381, 635, 872, 1005, 1034, 1056
BfuI GTATCC 3 cut(s) 160, 418, 1491
BglII AGATCT 2 cut(s) 133, 1030
BisI GCNGC 4 cut(s) 68, 236, 515, 1376
BlsI GCNGC 4 cut(s) 69, 237, 516, 1377
Bme1390I CCNGG 4 cut(s) 82, 260, 345, 725
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 338, 782
BmiI GGNNCC 1 cut(s) 783
BmrFI CCNGG 4 cut(s) 82, 260, 345, 725
BmsI GCATC 9 cut(s) 480, 841, 853, 901, 907, 1198, 1229, 1405, 1441
BpmI CTGGAG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 1187
BpuMI CCSGG 1 cut(s) 260
Bsa29I ATCGAT 1 cut(s) 1449
BsaBI GATNNNNATC 1 cut(s) 1035
BsaJI CCNNGG 6 cut(s) 153, 723, 724, 858, 1247, 1308
BsaWI WCCGGW 1 cut(s) 207
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 304, 350, 467
Bse118I RCCGGY 2 cut(s) 92, 1507
Bse1I ACTGG 4 cut(s) 606, 1100, 1224, 1276
Bse3DI GCAATG 1 cut(s) 612
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 3 cut(s) 82, 345, 725
BseCI ATCGAT 1 cut(s) 1449
BseDI CCNNGG 6 cut(s) 153, 723, 724, 858, 1247, 1308
BseGI GGATG 8 cut(s) 294, 400, 495, 868, 922, 1177, 1220, 1477
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 4 cut(s) 99, 304, 350, 467
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 3 cut(s) 279, 1221, 1509
BseNI ACTGG 4 cut(s) 606, 1100, 1224, 1276
BseRI GAGGAG 2 cut(s) 41, 639
BseXI GCAGC 3 cut(s) 222, 526, 1387
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 3 cut(s) 70, 477, 784
BshVI ATCGAT 1 cut(s) 1449
BsiSI CCGG 5 cut(s) 93, 139, 208, 259, 1508
BsiWI CGTACG 1 cut(s) 1334
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 4 cut(s) 99, 304, 350, 467
BsmFI GGGAC 1 cut(s) 595
BsnI GGCC 3 cut(s) 70, 477, 784
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 8 cut(s) 30, 133, 291, 366, 1030, 1036, 1122, 1446
Bsp19I CCATGG 2 cut(s) 1247, 1308
BspACI CCGC 5 cut(s) 67, 298, 392, 467, 914
BspANI GGCC 3 cut(s) 70, 477, 784
BspCNI CTCAG 3 cut(s) 280, 1222, 1510
BspDI ATCGAT 1 cut(s) 1449
BspFNI CGCG 1 cut(s) 300
BspHI TCATGA 1 cut(s) 1293
BspLI GGNNCC 1 cut(s) 783
BspPI GGATC 2 cut(s) 1031, 1117
BsrDI GCAATG 1 cut(s) 612
BsrFI RCCGGY 2 cut(s) 92, 1507
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 4 cut(s) 606, 1100, 1224, 1276
BssAI RCCGGY 2 cut(s) 92, 1507
BssECI CCNNGG 6 cut(s) 153, 723, 724, 858, 1247, 1308
BssMI GATC 8 cut(s) 30, 133, 291, 366, 1030, 1036, 1122, 1446
BssT1I CCWWGG 3 cut(s) 153, 1247, 1308
Bst2UI CCWGG 3 cut(s) 82, 345, 725
Bst4CI ACNGT 2 cut(s) 182, 859
Bst6I CTCTTC 2 cut(s) 1082, 1437
BstAUI TGTACA 1 cut(s) 15
BstC8I GCNNGC 3 cut(s) 11, 38, 144
BstDEI CTNAG 3 cut(s) 288, 1230, 1518
BstDSI CCRYGG 3 cut(s) 858, 1247, 1308
BstF5I GGATG 8 cut(s) 294, 400, 495, 868, 922, 1177, 1220, 1477
BstFNI CGCG 1 cut(s) 300
BstKTI GATC 8 cut(s) 33, 136, 294, 369, 1033, 1039, 1125, 1449
BstMBI GATC 8 cut(s) 30, 133, 291, 366, 1030, 1036, 1122, 1446
BstMWI GCNNNNNNNGC 5 cut(s) 19, 406, 872, 1217, 1375
BstNI CCWGG 3 cut(s) 82, 345, 725
BstNSI RCATGY 3 cut(s) 514, 801, 937
BstSCI CCNGG 4 cut(s) 80, 258, 343, 723
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 3 cut(s) 222, 526, 1387
BstX2I RGATCY 4 cut(s) 133, 1030, 1036, 1122
BstYI RGATCY 4 cut(s) 133, 1030, 1036, 1122
Bsu15I ATCGAT 1 cut(s) 1449
BsuI GTATCC 3 cut(s) 160, 418, 1491
BsuRI GGCC 3 cut(s) 70, 477, 784
BsuTUI ATCGAT 1 cut(s) 1449
BtgI CCRYGG 3 cut(s) 858, 1247, 1308
BtsCI GGATG 8 cut(s) 294, 400, 495, 868, 922, 1177, 1220, 1477
BtsI GCAGTG 1 cut(s) 1075
BtsIMutI CAGTG 2 cut(s) 1075, 1107
Cac8I GCNNGC 3 cut(s) 11, 38, 144
CaiI CAGNNNCTG 1 cut(s) 1517
CciI TCATGA 1 cut(s) 1293
Cfr10I RCCGGY 2 cut(s) 92, 1507
Cfr13I GGNCC 2 cut(s) 338, 782
ClaI ATCGAT 1 cut(s) 1449
Csp6I GTAC 5 cut(s) 16, 458, 609, 906, 1335
CviQI GTAC 5 cut(s) 16, 458, 609, 906, 1335
DdeI CTNAG 3 cut(s) 288, 1230, 1518
DpnI GATC 8 cut(s) 32, 135, 293, 368, 1032, 1038, 1124, 1448
DpnII GATC 8 cut(s) 30, 133, 291, 366, 1030, 1036, 1122, 1446
Eam1104I CTCTTC 2 cut(s) 1082, 1437
EarI CTCTTC 2 cut(s) 1082, 1437
EciI GGCGGA 1 cut(s) 407
Eco130I CCWWGG 3 cut(s) 153, 1247, 1308
Eco47I GGWCC 1 cut(s) 338
EcoO109I RGGNCCY 2 cut(s) 338, 782
EcoRII CCWGG 3 cut(s) 80, 343, 723
EcoT14I CCWWGG 3 cut(s) 153, 1247, 1308
EcoT22I ATGCAT 1 cut(s) 1456
ErhI CCWWGG 3 cut(s) 153, 1247, 1308
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 1 cut(s) 595
FauI CCCGC 1 cut(s) 460
FauNDI CATATG 2 cut(s) 402, 549
FbaI TGATCA 1 cut(s) 366
FblI GTMKAC 1 cut(s) 938
Fnu4HI GCNGC 4 cut(s) 68, 236, 515, 1376
FokI GGATG 8 cut(s) 281, 407, 502, 875, 929, 1184, 1207, 1464
Fsp4HI GCNGC 4 cut(s) 68, 236, 515, 1376
FspBI CTAG 6 cut(s) 381, 635, 872, 1005, 1034, 1056
GluI GCNGC 4 cut(s) 68, 236, 515, 1376
GsuI CTGGAG 1 cut(s) 366
HaeIII GGCC 3 cut(s) 70, 477, 784
HapII CCGG 5 cut(s) 93, 139, 208, 259, 1508
HinfI GANTC 6 cut(s) 679, 926, 1016, 1252, 1339, 1352
HpaII CCGG 5 cut(s) 93, 139, 208, 259, 1508
HphI GGTGA 3 cut(s) 1035, 1089, 1168
Hpy166II GTNNAC 4 cut(s) 508, 939, 1097, 1530
Hpy188III TCNNGA 7 cut(s) 279, 770, 1034, 1056, 1294, 1321, 1343
Hpy8I GTNNAC 4 cut(s) 508, 939, 1097, 1530
Hpy99I CGWCG 1 cut(s) 906
HpyAV CCTTC 3 cut(s) 693, 1050, 1318
HpyCH4III ACNGT 2 cut(s) 182, 859
HpyCH4IV ACGT 1 cut(s) 504
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 406, 872, 1217, 1375
HpyF3I CTNAG 3 cut(s) 288, 1230, 1518
HpySE526I ACGT 1 cut(s) 504
Ksp22I TGATCA 1 cut(s) 366
Kzo9I GATC 8 cut(s) 30, 133, 291, 366, 1030, 1036, 1122, 1446
Lsp1109I GCAGC 3 cut(s) 222, 526, 1387
LweI GCATC 9 cut(s) 480, 841, 853, 901, 907, 1198, 1229, 1405, 1441
MaeI CTAG 6 cut(s) 381, 635, 872, 1005, 1034, 1056
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 3 cut(s) 718, 985, 1183
MalI GATC 8 cut(s) 32, 135, 293, 368, 1032, 1038, 1124, 1448
MboI GATC 8 cut(s) 30, 133, 291, 366, 1030, 1036, 1122, 1446
MboII GAAGA 9 cut(s) 16, 143, 746, 1040, 1071, 1096, 1099, 1421, 1454
MflI RGATCY 4 cut(s) 133, 1030, 1036, 1122
MluCI AATT 9 cut(s) 375, 576, 765, 1176, 1212, 1268, 1387, 1404, 1523
MlyI GAGTC 2 cut(s) 920, 1025
MmeI TCCRAC 1 cut(s) 619
Mph1103I ATGCAT 1 cut(s) 1456
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 2 cut(s) 796, 1102
MspI CCGG 5 cut(s) 93, 139, 208, 259, 1508
MspR9I CCNGG 4 cut(s) 82, 260, 345, 725
MvaI CCWGG 3 cut(s) 82, 345, 725
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 5 cut(s) 19, 406, 872, 1217, 1375
NciI CCSGG 1 cut(s) 260
NcoI CCATGG 2 cut(s) 1247, 1308
NdeI CATATG 2 cut(s) 402, 549
NdeII GATC 8 cut(s) 30, 133, 291, 366, 1030, 1036, 1122, 1446
NlaIV GGNNCC 1 cut(s) 783
NmuCI GTSAC 1 cut(s) 718
NsiI ATGCAT 1 cut(s) 1456
NspI RCATGY 3 cut(s) 514, 801, 937
OliI CACNNNNGTG 1 cut(s) 1102
PagI TCATGA 1 cut(s) 1293
PasI CCCWGGG 1 cut(s) 724
PciI ACATGT 1 cut(s) 797
PfeI GAWTC 4 cut(s) 679, 1252, 1339, 1352
Pfl23II CGTACG 1 cut(s) 1334
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 4 cut(s) 69, 237, 516, 1377
PleI GAGTC 2 cut(s) 920, 1024
PpsI GAGTC 2 cut(s) 920, 1024
PpuMI RGGWCCY 1 cut(s) 338
PscI ACATGT 1 cut(s) 797
PshBI ATTAAT 1 cut(s) 192
PsiI TTATAA 1 cut(s) 566
Psp5II RGGWCCY 1 cut(s) 338
Psp6I CCWGG 3 cut(s) 80, 343, 723
PspGI CCWGG 3 cut(s) 80, 343, 723
PspLI CGTACG 1 cut(s) 1334
PspN4I GGNNCC 1 cut(s) 783
PspPI GGNCC 2 cut(s) 338, 782
PspPPI RGGWCCY 1 cut(s) 338
PstNI CAGNNNCTG 1 cut(s) 1517
PsuI RGATCY 4 cut(s) 133, 1030, 1036, 1122
RsaI GTAC 5 cut(s) 17, 459, 610, 907, 1336
RsaNI GTAC 5 cut(s) 16, 458, 609, 906, 1335
RseI CAYNNNNRTG 2 cut(s) 796, 1102
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 4 cut(s) 68, 236, 515, 1376
Sau3AI GATC 8 cut(s) 30, 133, 291, 366, 1030, 1036, 1122, 1446
Sau96I GGNCC 2 cut(s) 338, 782
SchI GAGTC 2 cut(s) 920, 1025
ScrFI CCNGG 4 cut(s) 82, 260, 345, 725
SfaNI GCATC 9 cut(s) 480, 841, 853, 901, 907, 1198, 1229, 1405, 1441
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 2 cut(s) 796, 1102
SmlI CTYRAG 1 cut(s) 1166
SmoI CTYRAG 1 cut(s) 1166
Sse9I AATT 9 cut(s) 375, 576, 765, 1176, 1212, 1268, 1387, 1404, 1523
SsiI CCGC 5 cut(s) 67, 298, 392, 467, 914
SspMI CTAG 6 cut(s) 381, 635, 872, 1005, 1034, 1056
StyD4I CCNGG 4 cut(s) 80, 258, 343, 723
StyI CCWWGG 3 cut(s) 153, 1247, 1308
TaaI ACNGT 2 cut(s) 182, 859
TaiI ACGT 1 cut(s) 507
TaqI TCGA 4 cut(s) 431, 1449, 1545, 1556
TasI AATT 9 cut(s) 375, 576, 765, 1176, 1212, 1268, 1387, 1404, 1523
TatI WGTACW 1 cut(s) 15
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 4 cut(s) 679, 1252, 1339, 1352
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 2 cut(s) 1075, 1107
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 3 cut(s) 235, 514, 1375
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 8 cut(s) 17, 419, 588, 747, 827, 1188, 1250, 1310
TspRI CASTG 2 cut(s) 1075, 1107
VpaK11BI GGWCC 1 cut(s) 338
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 1 cut(s) 1268
XbaI TCTAGA 2 cut(s) 1033, 1055
XceI RCATGY 3 cut(s) 514, 801, 937
XmiI GTMKAC 1 cut(s) 938
XspI CTAG 6 cut(s) 381, 635, 872, 1005, 1034, 1056
Zsp2I ATGCAT 1 cut(s) 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.