Rh1CG287200

ATPase family associated with various cellular activities (AAA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0008253
Physical Location & Seq
Reverse (-)
55931150 .. 55935320
4171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG287200.1

Sequence Viewer

Length: 1659 bp
ATGTGTTTACTTATTGACATTGTGGCTGCAACTTTTTTTTTTTTTGGTTTGCAGGTTAGTGAGTTTTCAGATGACAACCGTTCTGGTATTAATAACTCATTACACCGGATCAGTGCTATCCGAAACCGAAAGCTGCAAATCATTGGACTTACTTGCCGGGTGGGGAGAGCTGTGTCAGGAAGTGCGGAGATTATCCGTGACTTGGTTGAGGGTGGTGGTTCAATCTTGGTCATAGGACCTCCTGGAGTTGGCAAGACAACCTTGATCAGAGAAATTGCTAGAATGTTGGCGGATGAGCATATGAAGCGTGTTGTCATTGTGGATACATCGAATGAGATTGGGGGAGATGGAGATGTACCTCATGCGGGAATAGGGCGTGCAAGGAGGATGCAAGTTCCTAATGTGAACATGCAGCATAATGTTATGATTGAAGCAGTTGAAAATCATATGCCTCAAACCATTATAATAGATGAAATTGGGACAGAGCTTGAAGCATTGGCTTCCAGTACTATTGCTCAAAGAGGAGTTCAGCTAGTTGGAACAGCACATGGAATGACGATAGACAACATAATAAAGAATCCTTCTTTACAAATCCTTGTTGGTGGCATAGAGAGTGTGACCCTGGGAGATGAAGAAGCAAGAAAGAGAAAAGTTCAAAAGACAATTCTTGAAAGAAAGGGGCCTCCTACATTTACATGTGCTGTTGAGATTATATCTAAAACAGAGTTTCGTGTTCATCACAGATTAGATGCAACCGTGGATGCTATACTAGCTGGAAAATCTCCTCTGTTTGAAGTTCGTCGTACTGATGCGGATGCTGATGACTCTTGCATGTCTACTCAAATATCTGAACTGAACTATCAAAAAGTATCAGATTTTGGTGTTACTAAAGACATTATTGCTAGTGTTTTGGAGTCTGACGAGGAAGATCTAGATCCTTCACCCAATGTGTCTAGAAGATTGAGCAGTGATAAATCTGTGAAGAAGAGAAGTTCACCAGTGTGTATATATACATACAAGATCCTTGAAGCTGACCTACTGCAAGTAGCACAGGTGATGGGGCTTGAGGATGAAATTGAGGTAACTGATGATATTGGAACAGCAGTTGCAATTATAGCATCCAGTTCTGAGATGAAGCAAAACCCATGGATTCGTGGTGTAGCAAAATTCCACCAGTTGCCTGTATTTGTCATGAAGTCAAATACCATGGCACAAATCGTGAAGGCAGTTCGTACGATTCTTGAAATGGAATCAGTTGGTTCTTTGCCAAAGCAGCTTGTCAATAATTCCTTTGACATTGAAATTGAAGATGATGCACCCAAAAGAAAACCATCTTTAGAAGAGATCGATGCATTGGAGGAGGTTCGACTTGCAATCGAGTATATAGTGATTCCTGGTGGAGAGCCCGTAGAGCTCCTTCCAAGACGCTCCGAAATAATTGCCAGACAGCTGGAGCTGGTGGAAAGTTATCAGTTGGCTGCTGAAAATTCAGGTACTAAGCGCAATCCGAGGTTGCAGATTCTTCCCATGAGGATAAACAAGAAGAGTTCTTCGAAACCTGTTAAATCCAGTTCTATGAAACCGACTAACTCTAAATCACTAACCGGTGGTGGTGGAGGTAGCAGTGTTACTAGGCTCCCCCTTTTATCAGAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

552

Amino Acids

60.35

Weight (kDa)

5.68

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spore_III_AA PF19568 47 - 200 3.2e-13 Sporulation stage III, protein AA
AAA_16 PF13191 68 - 103 8.9e-06 AAA ATPase domain
AAA PF00004 75 - 160 5.7e-06 ATPase family associated with various cellular activities (AAA)
PTPase PF25516 334 - 414 3.2e-26 Phosphotyrosine protein phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 836
AciI CCGC 4 cut(s) 185, 290, 365, 812
AclWI GGATC 3 cut(s) 116, 929, 1015
AcsI RAATTY 2 cut(s) 1166, 1486
AfaI GTAC 5 cut(s) 357, 508, 805, 1234, 1495
AfiI CCNNNNNNNGG 3 cut(s) 202, 248, 365
AflIII ACRYGT 1 cut(s) 695
AgeI ACCGGT 1 cut(s) 1604
AjnI CCWGG 3 cut(s) 241, 621, 1393
AleI CACNNNNGTG 1 cut(s) 1000
Alw21I GWGCWC 1 cut(s) 1416
AlwI GGATC 3 cut(s) 116, 929, 1015
AoxI GGCC 1 cut(s) 680
ApeKI GCWGC 5 cut(s) 26, 133, 412, 1273, 1478
ApoI RAATTY 2 cut(s) 1166, 1486
AseI ATTAAT 1 cut(s) 90
AsiGI ACCGGT 1 cut(s) 1604
AspLEI GCGC 1 cut(s) 1503
AspS9I GGNCC 2 cut(s) 236, 680
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 933, 987, 1066
AsuII TTCGAA 1 cut(s) 1553
AvaII GGWCC 1 cut(s) 236
BanII GRGCYC 2 cut(s) 1407, 1416
BarI GAAGNNNNNNTAC 4 cut(s) 1020, 1052, 1401, 1433
Bbv12I GWGCWC 1 cut(s) 1416
BbvI GCAGC 5 cut(s) 13, 120, 424, 1285, 1465
BccI CCATC 3 cut(s) 341, 1051, 1339
BcgI CGANNNNNNTGC 2 cut(s) 1421, 1455
BciT130I CCWGG 3 cut(s) 243, 623, 1395
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 264
BcnI CCSGG 1 cut(s) 158
BfaI CTAG 7 cut(s) 279, 533, 770, 903, 932, 954, 1632
BfuAI ACCTGC 1 cut(s) 43
BfuI GTATCC 1 cut(s) 316
BglII AGATCT 1 cut(s) 928
BisI GCNGC 5 cut(s) 27, 134, 413, 1274, 1479
BlsI GCNGC 5 cut(s) 28, 135, 414, 1275, 1480
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 4 cut(s) 158, 243, 623, 1395
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 236, 680
BmiI GGNNCC 2 cut(s) 681, 1637
BmrFI CCNGG 4 cut(s) 158, 243, 623, 1395
BmsI GCATC 8 cut(s) 378, 739, 751, 799, 805, 1127, 1303, 1339
BpmI CTGGAG 2 cut(s) 264, 1472
Bpu14I TTCGAA 1 cut(s) 1553
BpuEI CTTGAG 1 cut(s) 1085
BpuMI CCSGG 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 1347
BsaBI GATNNNNATC 1 cut(s) 933
BsaJI CCNNGG 6 cut(s) 621, 622, 756, 1145, 1206, 1508
BsaWI WCCGGW 2 cut(s) 105, 1604
BsaXI ACNNNNNCTCC 2 cut(s) 1392, 1422
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 248, 365
Bse118I RCCGGY 1 cut(s) 1604
Bse1I ACTGG 5 cut(s) 504, 998, 1122, 1174, 1569
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 3 cut(s) 243, 623, 1395
BseCI ATCGAT 1 cut(s) 1347
BseDI CCNNGG 6 cut(s) 621, 622, 756, 1145, 1206, 1508
BseGI GGATG 6 cut(s) 298, 393, 766, 820, 1075, 1118
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 3 cut(s) 202, 248, 365
BseMII CTCAG 1 cut(s) 1119
BseNI ACTGG 5 cut(s) 504, 998, 1122, 1174, 1569
BseRI GAGGAG 3 cut(s) 537, 774, 1373
BseXI GCAGC 5 cut(s) 13, 120, 424, 1285, 1465
BshFI GGCC 1 cut(s) 682
BshTI ACCGGT 1 cut(s) 1604
BshVI ATCGAT 1 cut(s) 1347
BsiHKAI GWGCWC 1 cut(s) 1416
BsiSI CCGG 3 cut(s) 106, 157, 1605
BsiWI CGTACG 1 cut(s) 1232
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 202, 248, 365
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 1 cut(s) 682
Bsp119I TTCGAA 1 cut(s) 1553
Bsp1286I GDGCHC 2 cut(s) 1407, 1416
Bsp143I GATC 6 cut(s) 108, 264, 928, 934, 1020, 1344
Bsp19I CCATGG 2 cut(s) 1145, 1206
BspACI CCGC 4 cut(s) 185, 290, 365, 812
BspANI GGCC 1 cut(s) 682
BspCNI CTCAG 1 cut(s) 1120
BspDI ATCGAT 1 cut(s) 1347
BspHI TCATGA 1 cut(s) 1191
BspLI GGNNCC 2 cut(s) 681, 1637
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 3 cut(s) 116, 929, 1015
BspT104I TTCGAA 1 cut(s) 1553
BsrFI RCCGGY 1 cut(s) 1604
BsrI ACTGG 5 cut(s) 504, 998, 1122, 1174, 1569
BssAI RCCGGY 1 cut(s) 1604
BssECI CCNNGG 6 cut(s) 621, 622, 756, 1145, 1206, 1508
BssMI GATC 6 cut(s) 108, 264, 928, 934, 1020, 1344
BssT1I CCWWGG 2 cut(s) 1145, 1206
Bst2UI CCWGG 3 cut(s) 243, 623, 1395
Bst4CI ACNGT 2 cut(s) 80, 757
Bst6I CTCTTC 3 cut(s) 980, 1335, 1538
BstBI TTCGAA 1 cut(s) 1553
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 2 cut(s) 1128, 1497
BstDSI CCRYGG 3 cut(s) 756, 1145, 1206
BstF5I GGATG 6 cut(s) 298, 393, 766, 820, 1075, 1118
BstHHI GCGC 1 cut(s) 1503
BstKTI GATC 6 cut(s) 111, 267, 931, 937, 1023, 1347
BstMBI GATC 6 cut(s) 108, 264, 928, 934, 1020, 1344
BstMWI GCNNNNNNNGC 5 cut(s) 304, 770, 1115, 1273, 1411
BstNI CCWGG 3 cut(s) 243, 623, 1395
BstNSI RCATGY 3 cut(s) 412, 699, 835
BstSCI CCNGG 4 cut(s) 156, 241, 621, 1393
BstV1I GCAGC 5 cut(s) 13, 120, 424, 1285, 1465
BstX2I RGATCY 3 cut(s) 928, 934, 1020
BstXI CCANNNNNNTGG 1 cut(s) 1450
BstYI RGATCY 3 cut(s) 928, 934, 1020
Bsu15I ATCGAT 1 cut(s) 1347
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 1 cut(s) 682
BsuTUI ATCGAT 1 cut(s) 1347
BtgI CCRYGG 3 cut(s) 756, 1145, 1206
BtsCI GGATG 6 cut(s) 298, 393, 766, 820, 1075, 1118
BtsI GCAGTG 2 cut(s) 973, 1630
BtsIMutI CAGTG 4 cut(s) 118, 973, 1005, 1630
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 378
CciI TCATGA 1 cut(s) 1191
CfoI GCGC 1 cut(s) 1503
Cfr10I RCCGGY 1 cut(s) 1604
Cfr13I GGNCC 2 cut(s) 236, 680
ClaI ATCGAT 1 cut(s) 1347
CseI GACGC 1 cut(s) 1434
Csp6I GTAC 5 cut(s) 356, 507, 804, 1233, 1494
CspAI ACCGGT 1 cut(s) 1604
CviAII CATG 9 cut(s) 362, 409, 548, 696, 832, 1146, 1192, 1207, 1528
CviQI GTAC 5 cut(s) 356, 507, 804, 1233, 1494
DdeI CTNAG 2 cut(s) 1128, 1497
DpnI GATC 6 cut(s) 110, 266, 930, 936, 1022, 1346
DpnII GATC 6 cut(s) 108, 264, 928, 934, 1020, 1344
Eam1104I CTCTTC 3 cut(s) 980, 1335, 1538
EarI CTCTTC 3 cut(s) 980, 1335, 1538
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 1414
Eco130I CCWWGG 2 cut(s) 1145, 1206
Eco24I GRGCYC 2 cut(s) 1407, 1416
Eco47I GGWCC 1 cut(s) 236
Eco53kI GAGCTC 1 cut(s) 1414
EcoICRI GAGCTC 1 cut(s) 1414
EcoO109I RGGNCCY 2 cut(s) 236, 680
EcoRII CCWGG 3 cut(s) 241, 621, 1393
EcoT14I CCWWGG 2 cut(s) 1145, 1206
EcoT22I ATGCAT 1 cut(s) 1354
EcoT38I GRGCYC 2 cut(s) 1407, 1416
ErhI CCWWGG 2 cut(s) 1145, 1206
FaeI CATG 9 cut(s) 365, 412, 551, 699, 835, 1149, 1195, 1210, 1531
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 493
FatI CATG 9 cut(s) 361, 408, 547, 695, 831, 1145, 1191, 1206, 1527
FauI CCCGC 1 cut(s) 358
FauNDI CATATG 2 cut(s) 300, 447
FbaI TGATCA 1 cut(s) 264
FblI GTMKAC 1 cut(s) 836
Fnu4HI GCNGC 5 cut(s) 27, 134, 413, 1274, 1479
FokI GGATG 6 cut(s) 305, 400, 773, 827, 1082, 1105
FriOI GRGCYC 2 cut(s) 1407, 1416
Fsp4HI GCNGC 5 cut(s) 27, 134, 413, 1274, 1479
FspBI CTAG 7 cut(s) 279, 533, 770, 903, 932, 954, 1632
GlaI GCGC 1 cut(s) 1502
GluI GCNGC 5 cut(s) 27, 134, 413, 1274, 1479
GsuI CTGGAG 2 cut(s) 264, 1472
HaeIII GGCC 1 cut(s) 682
HapII CCGG 3 cut(s) 106, 157, 1605
HgaI GACGC 1 cut(s) 1434
HhaI GCGC 1 cut(s) 1503
Hin1II CATG 9 cut(s) 365, 412, 551, 699, 835, 1149, 1195, 1210, 1531
Hin6I GCGC 1 cut(s) 1501
HinP1I GCGC 1 cut(s) 1501
HinfI GANTC 8 cut(s) 577, 824, 914, 1150, 1237, 1250, 1390, 1519
HpaII CCGG 3 cut(s) 106, 157, 1605
HphI GGTGA 3 cut(s) 933, 987, 1066
Hpy166II GTNNAC 4 cut(s) 8, 406, 837, 995
Hpy188III TCNNGA 7 cut(s) 177, 668, 932, 954, 1192, 1219, 1241
Hpy8I GTNNAC 4 cut(s) 8, 406, 837, 995
Hpy99I CGWCG 1 cut(s) 804
HpyAV CCTTC 4 cut(s) 591, 948, 1216, 1427
HpyCH4III ACNGT 2 cut(s) 80, 757
HpyF10VI GCNNNNNNNGC 5 cut(s) 304, 770, 1115, 1273, 1411
HpyF3I CTNAG 2 cut(s) 1128, 1497
Hsp92II CATG 9 cut(s) 365, 412, 551, 699, 835, 1149, 1195, 1210, 1531
HspAI GCGC 1 cut(s) 1501
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 6 cut(s) 108, 264, 928, 934, 1020, 1344
LmnI GCTCC 4 cut(s) 1419, 1433, 1453, 1641
Lsp1109I GCAGC 5 cut(s) 13, 120, 424, 1285, 1465
LweI GCATC 8 cut(s) 378, 739, 751, 799, 805, 1127, 1303, 1339
MaeI CTAG 7 cut(s) 279, 533, 770, 903, 932, 954, 1632
MaeIII GTNAC 5 cut(s) 197, 616, 883, 1081, 1627
MalI GATC 6 cut(s) 110, 266, 930, 936, 1022, 1346
MboI GATC 6 cut(s) 108, 264, 928, 934, 1020, 1344
MflI RGATCY 3 cut(s) 928, 934, 1020
MhlI GDGCHC 2 cut(s) 1407, 1416
MlyI GAGTC 2 cut(s) 818, 923
MmeI TCCRAC 1 cut(s) 517
Mph1103I ATGCAT 1 cut(s) 1354
MseI TTAA 2 cut(s) 90, 1563
MslI CAYNNNNRTG 2 cut(s) 694, 1000
MspA1I CMGCKG 1 cut(s) 1450
MspI CCGG 3 cut(s) 106, 157, 1605
MspR9I CCNGG 4 cut(s) 158, 243, 623, 1395
MvaI CCWGG 3 cut(s) 243, 623, 1395
MwoI GCNNNNNNNGC 5 cut(s) 304, 770, 1115, 1273, 1411
NciI CCSGG 1 cut(s) 158
NcoI CCATGG 2 cut(s) 1145, 1206
NdeI CATATG 2 cut(s) 300, 447
NdeII GATC 6 cut(s) 108, 264, 928, 934, 1020, 1344
NlaIII CATG 9 cut(s) 365, 412, 551, 699, 835, 1149, 1195, 1210, 1531
NlaIV GGNNCC 2 cut(s) 681, 1637
NmuCI GTSAC 2 cut(s) 197, 616
NsiI ATGCAT 1 cut(s) 1354
NspI RCATGY 3 cut(s) 412, 699, 835
NspV TTCGAA 1 cut(s) 1553
OliI CACNNNNGTG 1 cut(s) 1000
PagI TCATGA 1 cut(s) 1191
PasI CCCWGGG 1 cut(s) 622
PciI ACATGT 1 cut(s) 695
PfeI GAWTC 6 cut(s) 577, 1150, 1237, 1250, 1390, 1519
Pfl23II CGTACG 1 cut(s) 1232
PfoI TCCNGGA 1 cut(s) 241
PinAI ACCGGT 1 cut(s) 1604
PkrI GCNGC 5 cut(s) 28, 135, 414, 1275, 1480
PleI GAGTC 2 cut(s) 818, 922
PpsI GAGTC 2 cut(s) 818, 922
PpuMI RGGWCCY 1 cut(s) 236
PscI ACATGT 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 90
PsiI TTATAA 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 1416
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 3 cut(s) 241, 621, 1393
PspGI CCWGG 3 cut(s) 241, 621, 1393
PspLI CGTACG 1 cut(s) 1232
PspN4I GGNNCC 2 cut(s) 681, 1637
PspPI GGNCC 2 cut(s) 236, 680
PspPPI RGGWCCY 1 cut(s) 236
PsuI RGATCY 3 cut(s) 928, 934, 1020
PvuII CAGCTG 1 cut(s) 1450
RsaI GTAC 5 cut(s) 357, 508, 805, 1234, 1495
RsaNI GTAC 5 cut(s) 356, 507, 804, 1233, 1494
RseI CAYNNNNRTG 2 cut(s) 694, 1000
SacI GAGCTC 1 cut(s) 1416
SaqAI TTAA 2 cut(s) 90, 1563
SatI GCNGC 5 cut(s) 27, 134, 413, 1274, 1479
Sau3AI GATC 6 cut(s) 108, 264, 928, 934, 1020, 1344
Sau96I GGNCC 2 cut(s) 236, 680
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 2 cut(s) 818, 923
ScrFI CCNGG 4 cut(s) 158, 243, 623, 1395
SduI GDGCHC 2 cut(s) 1407, 1416
SfaNI GCATC 8 cut(s) 378, 739, 751, 799, 805, 1127, 1303, 1339
SfuI TTCGAA 1 cut(s) 1553
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 2 cut(s) 694, 1000
SmlI CTYRAG 1 cut(s) 1064
SmoI CTYRAG 1 cut(s) 1064
SsiI CCGC 4 cut(s) 185, 290, 365, 812
SspMI CTAG 7 cut(s) 279, 533, 770, 903, 932, 954, 1632
SstI GAGCTC 1 cut(s) 1416
StyD4I CCNGG 4 cut(s) 156, 241, 621, 1393
StyI CCWWGG 2 cut(s) 1145, 1206
TaaI ACNGT 2 cut(s) 80, 757
TaqI TCGA 5 cut(s) 329, 1347, 1366, 1377, 1553
TatI WGTACW 1 cut(s) 506
TfiI GAWTC 6 cut(s) 577, 1150, 1237, 1250, 1390, 1519
Tru1I TTAA 2 cut(s) 90, 1563
Tru9I TTAA 2 cut(s) 90, 1563
TscAI CASTG 4 cut(s) 118, 973, 1005, 1630
TseFI GTSAC 2 cut(s) 197, 616
TseI GCWGC 5 cut(s) 26, 133, 412, 1273, 1478
Tsp45I GTSAC 2 cut(s) 197, 616
TspDTI ATGAA 8 cut(s) 317, 486, 645, 725, 1086, 1148, 1208, 1592
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 4 cut(s) 118, 973, 1005, 1630
VpaK11BI GGWCC 1 cut(s) 236
VspI ATTAAT 1 cut(s) 90
XapI RAATTY 2 cut(s) 1166, 1486
XbaI TCTAGA 2 cut(s) 931, 953
XceI RCATGY 3 cut(s) 412, 699, 835
XmiI GTMKAC 1 cut(s) 836
XspI CTAG 7 cut(s) 279, 533, 770, 903, 932, 954, 1632
ZrmI AGTACT 1 cut(s) 508
Zsp2I ATGCAT 1 cut(s) 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.