Rorug01G0297400

ATPase family associated with various cellular activities (AAA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008253
Physical Location & Seq
Forward (+)
41269453 .. 41275247
5795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0297400.1

Sequence Viewer

Length: 5073 bp
ATGTCTGAGATGGAGATGGCTTCACCGGAACCGGCGCCCATCCCGGTAGCTCTGAACTTCAAAGCCTCACTGATTGGGACTGAGGTTTGTGATCCCTCTGTGAGGGGGTTGGATGCTATCTCTCCAATCACAGCGGCTGATTATACGGTTTGTGAATTGGAGCGAGGTCCATCAATTCAATTTTCTGATAAGGTGGAAGAGGATTTATGCAAGCCTTGGCGTTATACTATCGTGCTCAAACCGCTGGGTAGGCCCATGACCTACAATTTTCTCCTGAATCGCCTCAAATCAAGATGGAATTTGAAAGGTCCTTGGGTTCTCATTGATATCCCCAATGATTTCTTTCTTGTCAAATTTGTGTTGAAGGAAGATATGGAATTTGTGCTAACAGGTGGACCACTGATGATTGCGGGACAATATGTGGCGCTGCAAAAATGGAAGTCTGATTTTGATTCAATGAACCATCGTGTTACCCATCTAGCTGTTTGGGTGCGGATTGCTGGACTGGATATCCGTTATTTCAAGCTTGATTCTCTTACTCAGATTGGTAATCTCCTTGGTGAGACTGTTAAAGTGGACCTGCATACTGCTGCTCAAGCAAGAGAAAGCAAAGAGAAGGATAGTGCCACTGTTATATCAACCAAAACTGCTACCGATGAGAATGTTGTGTCCAGGGAACCGACAGCTCCTGTCTCCAATTCTCCGGTGGATCAGAGTATTGCTGAAATGGTAAAAGATGTAGATGGTACCAATGTGGTAGGCCTTGGGCCATGGAATGATGTGAGCTATAAGAAAAAGAAGAATAATTTGAAGAATGAATCCTCTGGTAGCAGGAAGGTAGACAATCAAGGGTCTCGGTTCCAAGTGCTTGCAAATGACATTGAAGGATCATCTGTTCCTTCAGCCGGACTAGTGGCACCGACAAGCAGCCCTGCCATTCCAAAGACATGGAAACCAATCCAAGGGAAAACTAGTATGAAGAAAAGATCAGTTGAGATTGAGCAAAGTAATCAGCCAATGCTGAATGTGAAACTATCAGTCAAATCCAGAGGAAAAAATAAAAGCAAAGCTGCTACGGCTGAACAAGCTGCTCAGGTTACCTTTCCCCCTATTACTGAGCGAGCAACAAGTGATGCTGATCCAAGTCAGGCTCTTAACTCATCTGCTCCTTCTCCCTCAAAGGCCCCTGACCCAAATGATGTTGTGAGTACTCTACTGGGGAAGGATGAGAATGTTTTCATGGGCGGTACAATGTCATTGGTGTGTGCTAGCTCATCTGGGGTAAGTAAAGATATTATTTCTGAAGATGGTAGTTCTAATGCAGATATGGTAGGTGCTGCAGACTCCAAACTGCCTGATATTATTCAGGATTTGAGAAGAATATATAGATTTAATGTTTTGGCGATTTCTGAGCCTCGAATTAGTGGAGACAAAGCTAAAAATGTTGCAAAATCTTTGGGCTTTTCGTCTTTTGCTATTGAGGATGCTAATGGATTTTCTGGTGGTCTTTGGCTTTTATGGGATGAACGACATGTGAAAATTGAAGTGGTGGATAGTAATTTTCAGTCTATTACCTCTCTTGTCACTGAATCTGCAGATAAGCAATGGTTCTTCACTGCTGTTTATGCGAGTCCTGATCCTGTTTTACGTGAAGAGTTATGGTCCTATTTGGATAATTTTAACTCCCATTGTTCTATCTCTTGGCTTATTGCTGGTGATTTTAATGAGTTAATTTCAGATGCGGATAAACAAGGGGGACGAGCTGTTCTTGGTGGGGGTAGTTTTGCAACTTGGGTTGATAGAAACTTCATGATTGATATGGGCTACATTGGTGCTGATTTTACTTGGAAGGCTACCAGAAATGGGGAAGAGATTTTGGAGAGATTGGATAGAGGCATTTGTAATATTTCGTGGAGACATGAGTTCCCAGATGCTTTTGTTCAGCACGTTGCAAGACTTAAATCTGATCATTGTCCTATTTTTCTCAGTTTGAGTAGAGCTCAAACTCCTTCTCTAACTCTCAAACCTTTCCGTTTTGAGGCAATGTGGCTGAATCACTCGGGGTTTGGAGAATTTGCAAAGCAAAATTGGTCTATGGAGGAAGTTTCAATCTCCAACAAAATGGTCTTGTTTACAGATGCAGTGAAGAGATGGAATGTGGAAGTTTTTGGGAATATCTTTAGGAAAAAAAGAAGATTGATTGCTCGCCTTGCGGGTATTCAAAAGGTCCTCTGCCAAAGGAAGTCTCCTTTTCTTGTGAATCTGGAGAAGGAATTGACGAAAGAATATCATGAGATTTTGTCCAGTGAAGAGATTTTCTGGAAACAAAAATCGAGAAATACATGGCTGAGAGAGGGGGATAAGAACACCAAATTCTTTCATCTGTCCACCATTATTCGTAGAAGAAGAAACAGAATTGAAGGGCTTGAAGATGAGTATGGCACTTGGAGGACTGACAGAAATGATCTGCATTCTATTGCTGTTAGCTATTTCAAGGGATTATTCAGAGAAAAGAATACTTTAGGAGTAAGTGAAGCTATTCCTAATCTGTTCCCAAGAATTGATCCAAGCGATCTTGGTGACCTCAGTCGTGATGTTTCTGAATTAGAAATAAAGAGTAGTATGTTCTCCATTGGTGGGCTCAAAGCTCCCGGCCCTGATGGAATTCCTGCAAAGTTCTTCCAGGCTCATTGGTCAACATGCAAAGATGACATTATTAAGATGGTTAAAGAGTGCTTTGATAAATGTGAGCTCCCTGAGAACCTGAATAACACGCTCATTGCTTTAATTCCCAAAGTGGCAAGCCCTCTCTCCATGAAGCAGATGCGACCGATTAGCCTTTGCAACACTTTGTATAAAGTGGTCTCAAAGATCATTGTGCAAAGATTAAGGCCCATGATGAGTACGCTAGTCTCTCCTACTCAGGTTAGTTTTATTCCTGGCCGAAATATTACTGACAATATTGTTGTAGCTCAAGAGCTTTTGCATAAGTTTCGAAACTCCCGAGGGAACCTTGGGTTTATGGCTTGGAAGATTGATCTTGCTAAAGCTTATGACAAGCTACAGTGGCATTTTATTAAAAGTGTGCTATGGGAAGTGGGTATTCGGGGTAAATTATTACAGCTGATGATTCAGTGTTTTACCTCTGTCAATTCCAGAGTCATAATTAATGGAGAAGCCACTGAAAATATCAGTCCAGAGAGTGGGCTAAGACAGGGTGATCCTTTGTCCCCTTACCTGTTTGTCTTATGTATGGAGAAGCTTTCGCATTTAATTAATTTTCAAGTAGAACAAGGTACTTGGAAACCGGTGAAAATCTCTAGAAATGGCCCTGCTGTCTCCCATCTTTTCTTTGCAGATGACTTGATCTTATTTGCAGAGGCTTCTCTTTCTCAAGCTGAGGTAATGCGTTCTTGTATTGATTGCTTCTGCTTAAATTCAGGCCAAGAAGTAAGTTTTGAAAAATCCTCCCTTTTCTGTTCTCCAAATACTGAGGAAGGGCTTGCTGCTCAATTATCAGACATTTGTGGTTCTCCATTAACTGATGACTTAGGGAAGTATCTTGGAGTGCCTCTGTTACACTCAAGAGTTACCCCTGAGACTTATAAAGGCCTAGTGGAGAAGGTCCAACTCAGACTCACTTCTTGGAAAAGTCACACCCTCTCGATGGCTGGGAGACACACCCTAATTCAATCGGTTACAGCTGGACTACCAATTTATACTATGCAATCAGTAAGACTTCCTATGTCCATTTGTAATACTCTTGATCGTTTGAACAGGAACTTTCTTTGGGGGCACACTGAAGAACAGAAGAAAATTCATCTTGTGAAGTGGGATACAGTCTGTAAACCTAAATGTTATGGTGGCTTGGGTTTGAAGAAGACATGTATGATGAATCAGGCTGTGCTAGCTAAAACAGGATGGAGGCTTCTGCAACAGGAACAAGGGTTGTGGGCCCAGGCTTTGAATAAAAAATATCTCAAAGATGATTGCATTTTGCGAAAGCTAACATCACCTTTTCCTTCTAGTTCTAGTACTTGGAAAGGCATCCTCTATGGAGCTCAACTGCTCCCTCAGGGGTCTAAATGGAGGATTGGATCTGGGACGCAAATTTCCTTCTGGAATGATAATTGGACAGGTCTTGGTGTTTTGGCTGCTATAGCTTTGAAGCCTATTCCTCCCAATCTCGAAGGGCTAAGAGTGTGTGATGTTTATTGTGGTGGATGGGATTTACACCGGGTGCAAGAATTTTTATCTGAGGATGTGAAGTTAAAAATTTTGAGTATGCATGCAGGAGGTATTCGCAGTGGTGATGACACGATCATTTGGAGGTTATCACAGAATGGTAACTTTTCTGTTAAAACTGCTTATCACTCTCTGTTTTTGTACTTGGAACACCAACCATGGCTATGGAACTTTATTTGGAAGCTTCAAGTCCCTCATAAGGTGAAAACCTTTTTATGGACCCTTGTGCATGATAGATTGCTAACGAATGACGGCAGAGTTCGTAGAGGTTTGGCTACTGATACTCGTTGTCCAAGATGCGGCTTCCCACTGGAATCCTTGAACCATCTTTTCAAAGACTGCAATATTTCTATGAGGATTTGGCCTGCCCTCCATCCTACAAGGATTATTTGTGCTGGAGGAGCCCTTCGAAATGATAAGGGGGAATGGATGAATGGATTTGCTGTCAAGTTGGGCATTGGTCAAGTTGTTGAGGCTGAACTTTGGGGGCTTCTTAAAGGAATGGAAATGGCTTGGCAGAATGAGTGTAGACAATTGAAAATTGAAATGGATTCACTTGTTGCGGTTAAGTTGGTTCTCTCTCCCATTGCTCATCTCCATCCTCTTTATAGTATTGTGGCAAGTTGTCAGGAACTCTTGAGTAGGGATTGGAATGTGTCCCTTATCCATGTTTACAGAGAATGTAATTCAGTGGCTGATTTGTTAGCAAATATTGGGCATGGGTTTGAGCCTGGATGCAGATTCTTTAGCTCTCCTCCTACTGATGTTGTGCCTCTTCTGGAAGCTGATCTATTGGGTTTGTCTAAACCAAGACTAATTGTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

1690

Amino Acids

189.5

Weight (kDa)

8.67

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 73 - 152 2.3e-12 Domain of unknown function (DUF4283)
RVT_1 PF00078 931 - 1191 1.7e-31 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 1451 - 1536 4.2e-20 zinc-binding in reverse transcriptase
RVT_3 PF13456 1547 - 1652 4.3e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3606
Acc36I ACCTGC 1 cut(s) 588
Acc65I GGTACC 1 cut(s) 746
AccB1I GGYRCC 3 cut(s) 34, 746, 916
AccB7I CCANNNNNTGG 1 cut(s) 3203
AccI GTMKAC 2 cut(s) 840, 4774
AciI CCGC 9 cut(s) 134, 242, 410, 493, 1245, 1742, 2213, 4545, 4808
AclWI GGATC 8 cut(s) 86, 717, 895, 1133, 1631, 2558, 3215, 4105
AcoI YGGCCR 1 cut(s) 2941
AcuI CTGAAG 3 cut(s) 885, 1323, 3822
AcyI GRCGYC 1 cut(s) 35
AdeI CACNNNGTG 1 cut(s) 4240
AfaI GTAC 7 cut(s) 748, 1210, 1249, 2905, 3298, 4036, 4388
AflIII ACRYGT 2 cut(s) 1531, 3884
AgeI ACCGGT 1 cut(s) 3307
AhlI ACTAGT 2 cut(s) 910, 971
AjnI CCWGG 5 cut(s) 671, 2682, 2938, 3957, 4975
AloI GAACNNNNNNTCC 4 cut(s) 879, 911, 1749, 1781
Alw21I GWGCWC 4 cut(s) 237, 2002, 2754, 4063
AlwI GGATC 8 cut(s) 86, 717, 895, 1133, 1631, 2558, 3215, 4105
AlwNI CAGNNNCTG 3 cut(s) 137, 689, 4940
Ama87I CYCGRG 2 cut(s) 2059, 3003
ApaI GGGCCC 1 cut(s) 3958
ApeKI GCWGC 8 cut(s) 427, 590, 927, 1070, 1088, 1337, 3506, 4154
AseI ATTAAT 2 cut(s) 3168, 3276
AsiGI ACCGGT 1 cut(s) 3307
Asp700I GAANNNNTTC 2 cut(s) 1235, 2538
Asp718I GGTACC 1 cut(s) 746
AspLEI GCGC 2 cut(s) 37, 427
AsuC2I CCSGG 3 cut(s) 44, 2652, 4238
AsuHPI GGTGA 9 cut(s) 15, 572, 1727, 2591, 3230, 3322, 4005, 4322, 4459
AsuII TTCGAA 2 cut(s) 2995, 4654
AsuNHI GCTAGC 2 cut(s) 1268, 3907
AvaI CYCGRG 2 cut(s) 2059, 3003
AvaII GGWCC 8 cut(s) 167, 308, 395, 577, 1662, 2227, 3625, 4464
AxyI CCTNAGG 1 cut(s) 4074
BaeGI GKGCMC 2 cut(s) 3798, 3958
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BanI GGYRCC 3 cut(s) 34, 746, 916
BanII GRGCYC 6 cut(s) 2002, 2643, 2754, 3958, 4063, 4651
BbsI GAAGAC 1 cut(s) 3887
Bbv12I GWGCWC 4 cut(s) 237, 2002, 2754, 4063
BbvCI CCTCAGC 1 cut(s) 3399
BbvI GCAGC 8 cut(s) 414, 577, 939, 1057, 1075, 1324, 3493, 4141
BceAI ACGGC 2 cut(s) 1092, 4513
BcgI CGANNNNNNTGC 2 cut(s) 2975, 3009
BciT130I CCWGG 5 cut(s) 673, 2684, 2940, 3959, 4977
BciVI GTATCC 1 cut(s) 3829
BclI TGATCA 1 cut(s) 1966
BcnI CCSGG 3 cut(s) 44, 2652, 4238
BcuI ACTAGT 2 cut(s) 910, 971
BfmI CTRYAG 4 cut(s) 1338, 1593, 3062, 4158
BfoI RGCGCY 2 cut(s) 38, 428
BfuAI ACCTGC 1 cut(s) 588
BfuI GTATCC 1 cut(s) 3829
BmcAI AGTACT 2 cut(s) 1210, 4036
Bme1390I CCNGG 8 cut(s) 44, 673, 2652, 2684, 2940, 3959, 4238, 4977
Bme18I GGWCC 8 cut(s) 167, 308, 395, 577, 1662, 2227, 3625, 4464
BmeT110I CYCGRG 2 cut(s) 2059, 3003
BmrFI CCNGG 8 cut(s) 44, 673, 2652, 2684, 2940, 3959, 4238, 4977
BmrI ACTGGG 1 cut(s) 1226
BmtI GCTAGC 2 cut(s) 1272, 3911
BmuI ACTGGG 1 cut(s) 1226
BoxI GACNNNNGTC 1 cut(s) 2586
BpiI GAAGAC 1 cut(s) 3887
BplI GAGNNNNNCTC 2 cut(s) 1984, 2016
BpmI CTGGAG 2 cut(s) 2285, 4662
Bpu10I CCTNAGC 2 cut(s) 1092, 3399
Bpu14I TTCGAA 2 cut(s) 2995, 4654
BpuEI CTTGAG 5 cut(s) 579, 2958, 3378, 3568, 4903
BpuMI CCSGG 3 cut(s) 44, 2652, 4238
BsaAI YACGTR 1 cut(s) 1649
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 2 cut(s) 858, 2869
BsaWI WCCGGW 3 cut(s) 25, 703, 3307
Bse118I RCCGGY 2 cut(s) 31, 3307
Bse1I ACTGG 4 cut(s) 510, 1221, 2304, 4560
Bse21I CCTNAGG 1 cut(s) 4074
Bse3DI GCAATG 4 cut(s) 1610, 2049, 2778, 4830
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 5 cut(s) 673, 2684, 2940, 3959, 4977
BseJI GATNNNNATC 1 cut(s) 1137
BseMI GCAATG 4 cut(s) 1610, 2049, 2778, 4830
BseNI ACTGG 4 cut(s) 510, 1221, 2304, 4560
BseRI GAGGAG 2 cut(s) 4659, 4989
BseSI GKGCMC 2 cut(s) 3798, 3958
BseXI GCAGC 8 cut(s) 414, 577, 939, 1057, 1075, 1324, 3493, 4141
BseYI CCCAGC 2 cut(s) 244, 3671
Bsh1285I CGRYCG 1 cut(s) 2831
BshNI GGYRCC 3 cut(s) 34, 746, 916
BshTI ACCGGT 1 cut(s) 3307
BsiEI CGRYCG 1 cut(s) 2831
BsiHKAI GWGCWC 4 cut(s) 237, 2002, 2754, 4063
BsiHKCI CYCGRG 2 cut(s) 2059, 3003
BsiSI CCGG 8 cut(s) 26, 32, 44, 704, 906, 2652, 3308, 4237
BslFI GGGAC 7 cut(s) 91, 426, 1770, 3214, 4117, 4421, 4888
BsmFI GGGAC 7 cut(s) 91, 426, 1770, 3214, 4117, 4421, 4888
BsmI GAATGC 1 cut(s) 2470
Bso31I GGTCTC 2 cut(s) 858, 2869
BsoBI CYCGRG 2 cut(s) 2059, 3003
Bsp119I TTCGAA 2 cut(s) 2995, 4654
Bsp120I GGGCCC 1 cut(s) 3954
Bsp1286I GDGCHC 8 cut(s) 237, 2002, 2643, 2754, 3798, 3958, 4063, 4651
Bsp19I CCATGG 2 cut(s) 770, 4403
BspACI CCGC 9 cut(s) 134, 242, 410, 493, 1245, 1742, 2213, 4545, 4808
BspHI TCATGA 2 cut(s) 1809, 2290
BspMAI CTGCAG 2 cut(s) 1342, 1597
BspMI ACCTGC 1 cut(s) 588
BspOI GCTAGC 2 cut(s) 1272, 3911
BspPI GGATC 8 cut(s) 86, 717, 895, 1133, 1631, 2558, 3215, 4105
BspT104I TTCGAA 2 cut(s) 2995, 4654
BspT107I GGYRCC 3 cut(s) 34, 746, 916
BspTNI GGTCTC 2 cut(s) 858, 2869
BsrDI GCAATG 4 cut(s) 1610, 2049, 2778, 4830
BsrFI RCCGGY 2 cut(s) 31, 3307
BsrI ACTGG 4 cut(s) 510, 1221, 2304, 4560
BssAI RCCGGY 2 cut(s) 31, 3307
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 8 cut(s) 215, 311, 556, 763, 770, 961, 3013, 4403
Bst2UI CCWGG 5 cut(s) 673, 2684, 2940, 3959, 4977
Bst4CI ACNGT 5 cut(s) 148, 568, 631, 3066, 3841
Bst6I CTCTTC 6 cut(s) 192, 1647, 1863, 2141, 2304, 5025
BstACI GRCGYC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 1649
BstBI TTCGAA 2 cut(s) 2995, 4654
BstDSI CCRYGG 2 cut(s) 770, 4403
BstEII GGTNACC 2 cut(s) 1096, 2579
BstENI CCTNNNNNAGG 1 cut(s) 100
BstH2I RGCGCY 2 cut(s) 38, 428
BstHHI GCGC 2 cut(s) 37, 427
BstMCI CGRYCG 1 cut(s) 2831
BstNI CCWGG 5 cut(s) 673, 2684, 2940, 3959, 4977
BstNSI RCATGY 4 cut(s) 1535, 2703, 3888, 4292
BstPAI GACNNNNGTC 1 cut(s) 2586
BstPI GGTNACC 2 cut(s) 1096, 2579
BstSCI CCNGG 8 cut(s) 42, 671, 2650, 2682, 2938, 3957, 4236, 4975
BstSFI CTRYAG 4 cut(s) 1338, 1593, 3062, 4158
BstSLI GKGCMC 2 cut(s) 3798, 3958
BstV1I GCAGC 8 cut(s) 414, 577, 939, 1057, 1075, 1324, 3493, 4141
BstV2I GAAGAC 1 cut(s) 3887
BstX2I RGATCY 1 cut(s) 4097
BstXI CCANNNNNNTGG 3 cut(s) 948, 2122, 4410
BstYI RGATCY 1 cut(s) 4097
Bsu36I CCTNAGG 1 cut(s) 4074
BsuI GTATCC 1 cut(s) 3829
BtgI CCRYGG 2 cut(s) 770, 4403
BtsI GCAGTG 3 cut(s) 1614, 2148, 4312
BveI ACCTGC 1 cut(s) 588
CaiI CAGNNNCTG 3 cut(s) 137, 689, 4940
CciI TCATGA 2 cut(s) 1809, 2290
CfoI GCGC 2 cut(s) 37, 427
Cfr10I RCCGGY 2 cut(s) 31, 3307
CseI GACGC 1 cut(s) 4114
Csp6I GTAC 7 cut(s) 747, 1209, 1248, 2904, 3297, 4035, 4387
CspAI ACCGGT 1 cut(s) 3307
CspCI CAANNNNNGTGG 2 cut(s) 3932, 3967
CviQI GTAC 7 cut(s) 747, 1209, 1248, 2904, 3297, 4035, 4387
DinI GGCGCC 1 cut(s) 36
DraIII CACNNNGTG 1 cut(s) 4240
EaeI YGGCCR 1 cut(s) 2941
Eam1104I CTCTTC 6 cut(s) 192, 1647, 1863, 2141, 2304, 5025
EarI CTCTTC 6 cut(s) 192, 1647, 1863, 2141, 2304, 5025
Ecl136II GAGCTC 3 cut(s) 2000, 2752, 4061
Eco130I CCWWGG 8 cut(s) 215, 311, 556, 763, 770, 961, 3013, 4403
Eco147I AGGCCT 2 cut(s) 762, 3612
Eco24I GRGCYC 6 cut(s) 2002, 2643, 2754, 3958, 4063, 4651
Eco31I GGTCTC 2 cut(s) 858, 2869
Eco32I GATATC 2 cut(s) 328, 511
Eco47I GGWCC 8 cut(s) 167, 308, 395, 577, 1662, 2227, 3625, 4464
Eco53kI GAGCTC 3 cut(s) 2000, 2752, 4061
Eco57I CTGAAG 3 cut(s) 885, 1323, 3822
Eco81I CCTNAGG 1 cut(s) 4074
Eco88I CYCGRG 2 cut(s) 2059, 3003
Eco91I GGTNACC 2 cut(s) 1096, 2579
EcoICRI GAGCTC 3 cut(s) 2000, 2752, 4061
EcoNI CCTNNNNNAGG 1 cut(s) 100
EcoO109I RGGNCCY 3 cut(s) 308, 1183, 2227
EcoO65I GGTNACC 2 cut(s) 1096, 2579
EcoRI GAATTC 1 cut(s) 2664
EcoRII CCWGG 5 cut(s) 671, 2682, 2938, 3957, 4975
EcoRV GATATC 2 cut(s) 328, 511
EcoT14I CCWWGG 8 cut(s) 215, 311, 556, 763, 770, 961, 3013, 4403
EcoT22I ATGCAT 1 cut(s) 4290
EcoT38I GRGCYC 6 cut(s) 2002, 2643, 2754, 3958, 4063, 4651
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
ErhI CCWWGG 8 cut(s) 215, 311, 556, 763, 770, 961, 3013, 4403
FaqI GGGAC 7 cut(s) 91, 426, 1770, 3214, 4117, 4421, 4888
FauI CCCGC 2 cut(s) 403, 2206
FbaI TGATCA 1 cut(s) 1966
FblI GTMKAC 2 cut(s) 840, 4774
FriOI GRGCYC 6 cut(s) 2002, 2643, 2754, 3958, 4063, 4651
GlaI GCGC 2 cut(s) 36, 426
GsaI CCCAGC 2 cut(s) 248, 3675
GsuI CTGGAG 2 cut(s) 2285, 4662
HaeII RGCGCY 2 cut(s) 38, 428
HapII CCGG 8 cut(s) 26, 32, 44, 704, 906, 2652, 3308, 4237
HgaI GACGC 1 cut(s) 4114
HhaI GCGC 2 cut(s) 37, 427
Hin1I GRCGYC 1 cut(s) 35
Hin6I GCGC 2 cut(s) 35, 425
HinP1I GCGC 2 cut(s) 35, 425
HincII GTYRAC 1 cut(s) 2697
HindII GTYRAC 1 cut(s) 2697
HindIII AAGCTT 4 cut(s) 524, 3048, 3260, 4427
HpaII CCGG 8 cut(s) 26, 32, 44, 704, 906, 2652, 3308, 4237
HphI GGTGA 9 cut(s) 15, 572, 1727, 2591, 3230, 3322, 4005, 4322, 4459
Hpy166II GTNNAC 9 cut(s) 395, 577, 841, 2133, 2388, 2697, 3848, 4775, 4918
Hpy8I GTNNAC 9 cut(s) 395, 577, 841, 2133, 2388, 2697, 3848, 4775, 4918
HpyCH4III ACNGT 5 cut(s) 148, 568, 631, 3066, 3841
HpyCH4IV ACGT 2 cut(s) 1648, 1947
HpySE526I ACGT 2 cut(s) 1648, 1947
Hsp92I GRCGYC 1 cut(s) 35
HspAI GCGC 2 cut(s) 35, 425
KasI GGCGCC 1 cut(s) 34
KpnI GGTACC 1 cut(s) 750
Ksp22I TGATCA 1 cut(s) 1966
LmnI GCTCC 8 cut(s) 160, 691, 1171, 2653, 2757, 4058, 4074, 4646
Lsp1109I GCAGC 8 cut(s) 414, 577, 939, 1057, 1075, 1324, 3493, 4141
MaeII ACGT 2 cut(s) 1648, 1947
MaeIII GTNAC 9 cut(s) 469, 1096, 1582, 2579, 3576, 3589, 3653, 3697, 4346
MfeI CAATTG 1 cut(s) 4778
MflI RGATCY 1 cut(s) 4097
MhlI GDGCHC 8 cut(s) 237, 2002, 2643, 2754, 3798, 3958, 4063, 4651
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 4 cut(s) 1337, 1639, 3168, 3630
MmeI TCCRAC 3 cut(s) 90, 2139, 3652
Mph1103I ATGCAT 1 cut(s) 4290
MroXI GAANNNNTTC 2 cut(s) 1235, 2538
MslI CAYNNNNRTG 2 cut(s) 1261, 4408
MspA1I CMGCKG 4 cut(s) 134, 244, 3124, 3704
MspI CCGG 8 cut(s) 26, 32, 44, 704, 906, 2652, 3308, 4237
MspR9I CCNGG 8 cut(s) 44, 673, 2652, 2684, 2940, 3959, 4238, 4977
MunI CAATTG 1 cut(s) 4778
Mva1269I GAATGC 1 cut(s) 2470
MvaI CCWGG 5 cut(s) 673, 2684, 2940, 3959, 4977
NarI GGCGCC 1 cut(s) 35
NciI CCSGG 3 cut(s) 44, 2652, 4238
NcoI CCATGG 2 cut(s) 770, 4403
NheI GCTAGC 2 cut(s) 1268, 3907
NmuCI GTSAC 3 cut(s) 1582, 2579, 3653
NsiI ATGCAT 1 cut(s) 4290
NspI RCATGY 4 cut(s) 1535, 2703, 3888, 4292
NspV TTCGAA 2 cut(s) 2995, 4654
PacI TTAATTAA 1 cut(s) 3276
PaeI GCATGC 1 cut(s) 4292
PagI TCATGA 2 cut(s) 1809, 2290
PceI AGGCCT 2 cut(s) 762, 3612
PciI ACATGT 2 cut(s) 1531, 3884
PcsI WCGNNNNNNNCGW 1 cut(s) 3001
PctI GAATGC 1 cut(s) 2470
PdmI GAANNNNTTC 2 cut(s) 1235, 2538
PflMI CCANNNNNTGG 1 cut(s) 3203
PinAI ACCGGT 1 cut(s) 3307
PleI GAGTC 4 cut(s) 1337, 1638, 3167, 3630
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 4 cut(s) 1337, 1638, 3167, 3630
Ppu21I YACGTR 1 cut(s) 1649
PpuMI RGGWCCY 2 cut(s) 308, 2227
PscI ACATGT 2 cut(s) 1531, 3884
PshAI GACNNNNGTC 1 cut(s) 2586
PshBI ATTAAT 2 cut(s) 3168, 3276
PsiI TTATAA 1 cut(s) 3606
Psp124BI GAGCTC 3 cut(s) 2002, 2754, 4063
Psp5II RGGWCCY 2 cut(s) 308, 2227
Psp6I CCWGG 5 cut(s) 671, 2682, 2938, 3957, 4975
PspEI GGTNACC 2 cut(s) 1096, 2579
PspFI CCCAGC 2 cut(s) 244, 3671
PspGI CCWGG 5 cut(s) 671, 2682, 2938, 3957, 4975
PspOMI GGGCCC 1 cut(s) 3954
PspPPI RGGWCCY 2 cut(s) 308, 2227
PstI CTGCAG 2 cut(s) 1342, 1597
PstNI CAGNNNCTG 3 cut(s) 137, 689, 4940
PsuI RGATCY 1 cut(s) 4097
PvuII CAGCTG 2 cut(s) 3124, 3704
RsaI GTAC 7 cut(s) 748, 1210, 1249, 2905, 3298, 4036, 4388
RsaNI GTAC 7 cut(s) 747, 1209, 1248, 2904, 3297, 4035, 4387
RseI CAYNNNNRTG 2 cut(s) 1261, 4408
SacI GAGCTC 3 cut(s) 2002, 2754, 4063
ScaI AGTACT 2 cut(s) 1210, 4036
SchI GAGTC 4 cut(s) 1337, 1639, 3168, 3630
ScrFI CCNGG 8 cut(s) 44, 673, 2652, 2684, 2940, 3959, 4238, 4977
SduI GDGCHC 8 cut(s) 237, 2002, 2643, 2754, 3798, 3958, 4063, 4651
SfcI CTRYAG 4 cut(s) 1338, 1593, 3062, 4158
SfoI GGCGCC 1 cut(s) 36
SfuI TTCGAA 2 cut(s) 2995, 4654
SinI GGWCC 8 cut(s) 167, 308, 395, 577, 1662, 2227, 3625, 4464
SmiMI CAYNNNNRTG 2 cut(s) 1261, 4408
SmlI CTYRAG 5 cut(s) 594, 2973, 3393, 3583, 4882
SmoI CTYRAG 5 cut(s) 594, 2973, 3393, 3583, 4882
SpeI ACTAGT 2 cut(s) 910, 971
SphI GCATGC 1 cut(s) 4292
SseBI AGGCCT 2 cut(s) 762, 3612
SsiI CCGC 9 cut(s) 134, 242, 410, 493, 1245, 1742, 2213, 4545, 4808
SspDI GGCGCC 1 cut(s) 34
SspI AATATT 5 cut(s) 1906, 2950, 2962, 4591, 4957
SstI GAGCTC 3 cut(s) 2002, 2754, 4063
StuI AGGCCT 2 cut(s) 762, 3612
StyD4I CCNGG 8 cut(s) 42, 671, 2650, 2682, 2938, 3957, 4236, 4975
StyI CCWWGG 8 cut(s) 215, 311, 556, 763, 770, 961, 3013, 4403
TaaI ACNGT 5 cut(s) 148, 568, 631, 3066, 3841
TaiI ACGT 2 cut(s) 1651, 1950
TaqI TCGA 6 cut(s) 1417, 2333, 2995, 3665, 4188, 4654
TaqII GACCGA 1 cut(s) 2845
TatI WGTACW 3 cut(s) 1208, 4034, 4386
TauI GCSGC 2 cut(s) 137, 4548
TseFI GTSAC 3 cut(s) 1582, 2579, 3653
TseI GCWGC 8 cut(s) 427, 590, 927, 1070, 1088, 1337, 3506, 4154
Tsp45I GTSAC 3 cut(s) 1582, 2579, 3653
TspGWI ACGGA 2 cut(s) 503, 2021
Van91I CCANNNNNTGG 1 cut(s) 3203
VpaK11BI GGWCC 8 cut(s) 167, 308, 395, 577, 1662, 2227, 3625, 4464
VspI ATTAAT 2 cut(s) 3168, 3276
XagI CCTNNNNNAGG 1 cut(s) 100
XbaI TCTAGA 1 cut(s) 3320
XceI RCATGY 4 cut(s) 1535, 2703, 3888, 4292
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmiI GTMKAC 2 cut(s) 840, 4774
XmnI GAANNNNTTC 2 cut(s) 1235, 2538
ZrmI AGTACT 2 cut(s) 1210, 4036
Zsp2I ATGCAT 1 cut(s) 4290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.