MD01G1102700.v1.1

Domain of unknown function (DUF3783)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010336
Physical Location & Seq
Forward (+)
21490035 .. 21493641
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1102700.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGCAGCTTCTGCTCTTGCTTTCTCTCTGAAAACTTCACTCAACAATTCTCCTCTGCTTTTTATCCCTTCAATTTCCTCAAATCCTCTGCTGCCTCCAAAGATTTCAACCCTTTCTTCTTCTTCGTCGTCGGAAAATTACAGAAGCCATCAGTTGAAGCTCCTCAAGAGCACCCCAAGAGCCTCTTCTGAAAGAGAAGGAGTTCCTGGTGAGTTGATTGAAGATTCAAAATTCGTTCCTCTAAATGCAGATGAAATGATATTTGGTCCACCTGCTTTGTTATTGTTGGGGTTTGAAGCGGAGGAAGCTGAGAAGATCCAACAGTTTTTGAAACAGCTAGATGGAGAATTCCTCAAGGTTATTTATTGTACTGAAGACATGATTACTCGTTCACTTTGGGACGCGATGAATACAAGCCAGCCAAATTTGGAAGCATTAAAGATTGCTAAGCCGCTGCCACGAATCTGTTTCTTCTCTGGTCTCAGTGGGGAGGAGATGATGGTGTTCATTGATTCTTTCCCGGAATCAGGACTCGAACCAGCCATTTTTGCAGCTCTTGTTCCCAACAGTGCCGATAAACCACTGGCGGAGTTGATAGAAGAGATCGTGGGGGACCATGAAATGCTAACTGCGAAAGAAAAGGAAAATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3783

Protein Analysis

218

Amino Acids

23.76

Weight (kDa)

4.62

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3783 PF12646 154 - 209 2.4e-17 Domain of unknown function (DUF3783)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 280
Acc36I ACCTGC 1 cut(s) 280
AccII CGCG 1 cut(s) 404
AciI CCGC 3 cut(s) 299, 452, 587
AclWI GGATC 1 cut(s) 310
AcsI RAATTY 3 cut(s) 230, 347, 424
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 1 cut(s) 370
AfiI CCNNNNNNNGG 1 cut(s) 527
AgsI TTSAA 7 cut(s) 72, 108, 157, 221, 228, 296, 331
AjnI CCWGG 1 cut(s) 205
AjuI GAANNNNNNNTTGG 4 cut(s) 169, 201, 246, 278
AluBI AGCT 5 cut(s) 8, 160, 308, 337, 554
AluI AGCT 5 cut(s) 8, 160, 308, 337, 554
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 1 cut(s) 310
AlwNI CAGNNNCTG 1 cut(s) 11
ApeKI GCWGC 4 cut(s) 5, 91, 454, 551
ApoI RAATTY 3 cut(s) 230, 347, 424
Asp700I GAANNNNTTC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 266, 613
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 1 cut(s) 221
AvaII GGWCC 2 cut(s) 266, 613
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 4 cut(s) 17, 78, 441, 563
BccI CCATC 3 cut(s) 156, 335, 493
BciT130I CCWGG 1 cut(s) 207
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 280
BisI GCNGC 5 cut(s) 6, 92, 452, 455, 552
BlpI GCTNAGC 1 cut(s) 447
BlsI GCNGC 5 cut(s) 7, 93, 453, 456, 553
Bme1390I CCNGG 2 cut(s) 207, 521
Bme18I GGWCC 2 cut(s) 266, 613
BmgT120I GGNCC 2 cut(s) 266, 613
BmiI GGNNCC 1 cut(s) 614
BmrFI CCNGG 2 cut(s) 207, 521
BpiI GAAGAC 1 cut(s) 381
BplI GAGNNNNNCTC 2 cut(s) 336, 368
Bpu1102I GCTNAGC 1 cut(s) 447
BpuEI CTTGAG 2 cut(s) 149, 338
BpuMI CCSGG 1 cut(s) 521
BsaI GGTCTC 1 cut(s) 485
Bsc4I CCNNNNNNNGG 1 cut(s) 527
Bse1I ACTGG 1 cut(s) 588
BseBI CCWGG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 527
BseMII CTCAG 2 cut(s) 300, 496
BseNI ACTGG 1 cut(s) 588
BseRI GAGGAG 3 cut(s) 42, 152, 506
BseXI GCAGC 4 cut(s) 17, 78, 441, 563
Bsh1236I CGCG 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 173
BsiSI CCGG 1 cut(s) 521
BslFI GGGAC 2 cut(s) 413, 626
BslI CCNNNNNNNGG 1 cut(s) 527
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 2 cut(s) 413, 626
Bso31I GGTCTC 1 cut(s) 485
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 2 cut(s) 315, 603
Bsp1720I GCTNAGC 1 cut(s) 447
BspACI CCGC 3 cut(s) 299, 452, 587
BspCNI CTCAG 2 cut(s) 301, 495
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 1 cut(s) 614
BspMI ACCTGC 1 cut(s) 280
BspPI GGATC 1 cut(s) 310
BspTNI GGTCTC 1 cut(s) 485
BsrI ACTGG 1 cut(s) 588
BssMI GATC 2 cut(s) 315, 603
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 2 cut(s) 324, 569
Bst6I CTCTTC 2 cut(s) 190, 594
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 419
BstDEI CTNAG 3 cut(s) 309, 447, 482
BstFNI CGCG 1 cut(s) 404
BstKTI GATC 2 cut(s) 318, 606
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 2 cut(s) 315, 603
BstMWI GCNNNNNNNGC 3 cut(s) 11, 305, 548
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 2 cut(s) 205, 519
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 4 cut(s) 17, 78, 441, 563
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BtgZI GCGATG 1 cut(s) 419
BtsIMutI CAGTG 3 cut(s) 490, 574, 581
BveI ACCTGC 1 cut(s) 280
Cac8I GCNNGC 1 cut(s) 419
CaiI CAGNNNCTG 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 266, 613
CseI GACGC 1 cut(s) 410
Csp6I GTAC 1 cut(s) 369
CspCI CAANNNNNGTGG 2 cut(s) 258, 293
CviAII CATG 2 cut(s) 379, 617
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 3 cut(s) 309, 447, 482
DpnI GATC 2 cut(s) 317, 605
DpnII GATC 2 cut(s) 315, 603
Eam1104I CTCTTC 2 cut(s) 190, 594
EarI CTCTTC 2 cut(s) 190, 594
EciI GGCGGA 1 cut(s) 602
Eco31I GGTCTC 1 cut(s) 485
Eco47I GGWCC 2 cut(s) 266, 613
Eco57I CTGAAG 1 cut(s) 393
EcoRI GAATTC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 652
FaeI CATG 2 cut(s) 382, 620
FaiI YATR 3 cut(s) 380, 618, 652
FalI AAGNNNNNCTT 2 cut(s) 169, 201
FaqI GGGAC 2 cut(s) 413, 626
FatI CATG 2 cut(s) 378, 616
Fnu4HI GCNGC 5 cut(s) 6, 92, 452, 455, 552
Fsp4HI GCNGC 5 cut(s) 6, 92, 452, 455, 552
FspBI CTAG 1 cut(s) 338
GluI GCNGC 5 cut(s) 6, 92, 452, 455, 552
HapII CCGG 1 cut(s) 521
HgaI GACGC 1 cut(s) 410
Hin1II CATG 2 cut(s) 382, 620
HinfI GANTC 5 cut(s) 224, 462, 512, 524, 531
HpaII CCGG 1 cut(s) 521
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 2 cut(s) 269, 392
Hpy188I TCNGA 3 cut(s) 30, 133, 190
Hpy188III TCNNGA 2 cut(s) 166, 528
Hpy8I GTNNAC 2 cut(s) 269, 392
Hpy99I CGWCG 2 cut(s) 130, 133
HpyAV CCTTC 2 cut(s) 78, 191
HpyCH4III ACNGT 2 cut(s) 324, 569
HpyCH4V TGCA 3 cut(s) 248, 551, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 305, 548
HpyF3I CTNAG 3 cut(s) 309, 447, 482
Hsp92II CATG 2 cut(s) 382, 620
Kzo9I GATC 2 cut(s) 315, 603
LmnI GCTCC 1 cut(s) 165
LpnPI CCDG 9 cut(s) 192, 219, 285, 431, 462, 513, 534, 552, 569
Lsp1109I GCAGC 4 cut(s) 17, 78, 441, 563
MaeI CTAG 1 cut(s) 338
MalI GATC 2 cut(s) 317, 605
MboI GATC 2 cut(s) 315, 603
MboII GAAGA 9 cut(s) 108, 111, 114, 177, 233, 325, 386, 463, 611
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 6 cut(s) 46, 72, 136, 230, 347, 424
MlyI GAGTC 1 cut(s) 525
MmeI TCCRAC 2 cut(s) 111, 343
Mph1103I ATGCAT 1 cut(s) 652
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 1 cut(s) 437
MspA1I CMGCKG 1 cut(s) 454
MspI CCGG 1 cut(s) 521
MspR9I CCNGG 2 cut(s) 207, 521
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 3 cut(s) 11, 305, 548
NciI CCSGG 1 cut(s) 521
NdeII GATC 2 cut(s) 315, 603
NlaIII CATG 2 cut(s) 382, 620
NlaIV GGNNCC 1 cut(s) 614
NsiI ATGCAT 1 cut(s) 652
PaqCI CACCTGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 4 cut(s) 224, 462, 512, 524
PfoI TCCNGGA 1 cut(s) 519
PkrI GCNGC 5 cut(s) 7, 93, 453, 456, 553
PleI GAGTC 1 cut(s) 525
PpsI GAGTC 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 614
PspPI GGNCC 2 cut(s) 266, 613
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 315
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 5 cut(s) 6, 92, 452, 455, 552
Sau3AI GATC 2 cut(s) 315, 603
Sau96I GGNCC 2 cut(s) 266, 613
SchI GAGTC 1 cut(s) 525
ScrFI CCNGG 2 cut(s) 207, 521
SduI GDGCHC 1 cut(s) 173
SetI ASST 7 cut(s) 10, 162, 274, 310, 339, 360, 556
SinI GGWCC 2 cut(s) 266, 613
SmlI CTYRAG 2 cut(s) 164, 353
SmoI CTYRAG 2 cut(s) 164, 353
Sse9I AATT 6 cut(s) 46, 72, 136, 230, 347, 424
SsiI CCGC 3 cut(s) 299, 452, 587
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 2 cut(s) 205, 519
TaaI ACNGT 2 cut(s) 324, 569
TaqI TCGA 1 cut(s) 534
TasI AATT 6 cut(s) 46, 72, 136, 230, 347, 424
TatI WGTACW 1 cut(s) 368
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 4 cut(s) 224, 462, 512, 524
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 3 cut(s) 490, 574, 588
TseI GCWGC 4 cut(s) 5, 91, 454, 551
TspDTI ATGAA 4 cut(s) 267, 422, 496, 633
TspRI CASTG 3 cut(s) 490, 574, 588
VpaK11BI GGWCC 2 cut(s) 266, 613
XapI RAATTY 3 cut(s) 230, 347, 424
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.