MD01G1111200.v1.1

MIZ/SP-RING zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000955
Physical Location & Seq
Reverse (-)
22373564 .. 22382370
8807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1111200.v1.1.491

Sequence Viewer

Length: 2307 bp
ATGGATGTGGTGGCTGAGTGCAAGAGAAAATTGGCATCCTTTCGAGTCAAAGAGCTTAAGGACGTTCTGACTCAGCTGGGCATGTCAAAGCAAGGGAGGAAACAGGATCTGGTTGATAGAATCTTAGCAGAAGTTTCAGATCAACATAGGTTCTTGGCAGCATCCAATGCACTCCATTCATCGAAGAAAAAAATAATTGAGAAGAAAGGAGTTGCAGAACTGATTGATGAAGCTTACAGAAAATTGCCGAATACATCGTCAGCTGATTCTGCTTCAAAGGAACAGAGTGGTTCAGACACCTGCAGTGTGAAACGTAAAAAGGAAGTTAAAACTTTTAAGGATCCAGATGTGAAGATTTGCTGCCCCTGTGGAAGTGCATTGTCTAATGGGTTGATGATCCAGTGTGTGGACTCAAGTTGTCAAGTGCAGCAACATCTTGGTTGTGTTATTATTCCAGAGGAAACAAGGGATTTCAATATACCAGTTCGGCCTCTATTCTACTGTGAACTGTGTCGTCTCAAACGAGCAGACCCTTTTTGGGCAACTGTGGTAGATCTACTATCTCCGGTGAAACTAGTTACTTCAAATATTCCAATTGATGGTGCAAATCCTACTCAGAGTGTAGAGAAGACATTTCTACTTTCTAGAGCTGATAGAGATCTACTAAAGGATAATCAATATGATGTTCAGGCTTGGTGTATACTTCTTAATGATAGTGTTTCATTTAGGATGCAATGGCCACAGTTTGCAGATTTACAGGTCAATGGTGTGAATGTTAGGACAGTGAATAGACCTGCACATCAATTGTTAGGTGCCAATGGTCGTGATGATGGGGCGTTAATCACAATATACACTGGGGAGGGAATCAATAAGATTTCGCTATCGGCATGTGATAATCGTGCTTTCTGTTTAGGTGTCAGACTAGTAAAGCGACGAACAGTTCAGCAGGTTCTCAACTTGATTCCTAAAGAAGAAGATGGTGAGCTTTTTGAAGATGCACTAGCACGAATTCGTCGTTGCATTGGCGGTGGGCCTGCTGTGGCAGCTGAAGATAGTGATAGTGACCTGGAAGTGATTTCAGACTCAATATCAGTAAATCTCCGCTGCCCTATGAGTGGCAGTCGAATGAAGGTAGCTGGCAGATTCAAACCTTGTGCCCACATGGGTTGCTTTGATCTTGAAACTTTTGTGGAGCTAAACCAACGCTCTAGGAAGTGGCAATGTCCTGTATGCCTGAAGAACTACTCTGTAGAGGACGTAATCATTGACCGTTATTTCAATCGCATTACAACTATGATGCGAAATTGTGGAGAAGACATAGCAGAGATCAATGTAAAGCCAGATGGTTCTTGGAGTGCAAAGACCAAAGGTGAGTTTGGTGATCTTGCGCAGTGGCATTTGCCCGATGGATCTCCATGTGGTGATAGTGGTTTAGAACCCTCGAGGAAGTTGAAGCTGGAATCTGGTTTAAACGAAGATAATGGAGTGAAGTATAATTGGGTTACTGAAGTTCGTGGGCATCAGCCTGACACCTTAGAGGAAGTATTTGAGGGCTGTAGTCACAATGTGATAACAATGAGCAGCAGTGACTCCAGCGGGAACCTTGATTTCTCTGTAAATGAGGACAATGAAATCAATTCTGATCCGACATTTTCAAATAAGAATGGTGATTCTGCTGAAGCTGGGAATGCTGACGTCATCATTCTTAGTGACTCCGAAGAAGAAGATGCCAATTTAGTTTCTTCTGGAACCACCTACAGTACATTTCCAGTAAGTGGAACAGATCCTGGGTTCCAATATTTTGGTAATGTCTCTGGTTCTTTCATTGATAGCACATTGGTTCCAGCGGATCTAAATCCAAGCAGCCAAGTTATGAACTCTTCTATGTTCCATGCTGATGCTGGATTGGACTTGATTGAAAATACCTTGCCCTTTGCTAGTGAGGACCCCTCACTGCAAAATTTTCTTCCAACTCAGCCCTCGGCTTTGTTGGAAGAGTGTGATTCTGGCCAACATCCTCCAGAATCAAACGGCATTAGTGGGGACGACTGGATCTCTCTTCGACTTGGCAGGGTTGATGAAAATGTTCCCAATGCCTGCCCTGAACATGTATCCACACATGAAGTACAGTTGAGTAACGAATGTGGATCAGATGAAGCTACTCCTCTAATTAAGGATGGAACTGATGAAGTTAGGTCTAACAAAGCAAAGTCACGGAAATTTTCAGACGGACCTTTCTCGTTTCCTCGTCAGCCAAGGACTTTGAGGCAACGAGTGTATCTACCAGTAGAATCCAACTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04630 ko05160 map04120 map04630 map05160
Pfam Domains
Protein Families
SAP zf-MIZ

Protein Analysis

769

Amino Acids

84.61

Weight (kDa)

4.96

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP PF02037 11 - 42 5.8e-08 SAP domain
zf-MIZ PF02891 365 - 413 2.2e-19 MIZ/SP-RING zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000955)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970
malus_domestica MD01G1111200.v1.1 MD07G1177400.v1.1
prunus_persica Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1
pyrus_communis pycom01g13860 pycom07g17140
rosa_chinensis RchiOBHm_Chr1g0363931
rosa_laevigata RLG00000027534
rosa_multiflora Rmu_sc0001607.1_g000006
rosa_roxburghii Rroxscaffold_4G00292830
rosa_rugosa Rorug01G0311200 Rorug01G0311300
rosa_samantha Rh1AG319200 Rh1BG282200 Rh1CG298700 Rh1DG313600
rosa_wichuraiana Rw1G028290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 308
AatII GACGTC 1 cut(s) 1698
AbsI CCTCGAGG 1 cut(s) 1441
Acc16I TGCGCA 1 cut(s) 1389
Acc36I ACCTGC 3 cut(s) 308, 802, 937
AccB1I GGYRCC 1 cut(s) 812
AccB7I CCANNNNNTGG 3 cut(s) 406, 599, 1775
AccI GTMKAC 1 cut(s) 700
AciI CCGC 4 cut(s) 1026, 1102, 1596, 1847
AcoI YGGCCR 2 cut(s) 737, 2008
AcsI RAATTY 3 cut(s) 1008, 1960, 2219
AcuI CTGAAG 4 cut(s) 1068, 1256, 1527, 1698
AcyI GRCGYC 1 cut(s) 1695
AdeI CACNNNGTG 1 cut(s) 1567
AfaI GTAC 2 cut(s) 1762, 2127
AfiI CCNNNNNNNGG 5 cut(s) 406, 538, 599, 1115, 1775
AflII CTTAAG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 2107
AhlI ACTAGT 2 cut(s) 574, 922
AjnI CCWGG 2 cut(s) 1065, 1786
Alw26I GTCTC 2 cut(s) 521, 1816
AlwNI CAGNNNCTG 2 cut(s) 109, 1787
Ama87I CYCGRG 1 cut(s) 1441
AoxI GGCC 4 cut(s) 488, 737, 1031, 2008
ApeKI GCWGC 7 cut(s) 158, 360, 427, 1043, 1104, 1581, 1863
ApoI RAATTY 3 cut(s) 1008, 1960, 2219
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
Asp700I GAANNNNTTC 1 cut(s) 2085
AspLEI GCGC 1 cut(s) 1390
AspS9I GGNCC 3 cut(s) 1031, 1945, 2231
AsuHPI GGTGA 6 cut(s) 580, 992, 1382, 1391, 1433, 1679
AvaI CYCGRG 1 cut(s) 1441
AvaII GGWCC 2 cut(s) 1945, 2231
BaeGI GKGCMC 1 cut(s) 1159
BaeI ACNNNNGTAYC 2 cut(s) 2261, 2294
BalI TGGCCA 2 cut(s) 739, 2010
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 812
BbsI GAAGAC 2 cut(s) 635, 1320
BbvI GCAGC 7 cut(s) 170, 347, 439, 1055, 1091, 1593, 1875
BccI CCATC 6 cut(s) 593, 824, 971, 1337, 1400, 2171
BceAI ACGGC 1 cut(s) 2047
BciT130I CCWGG 2 cut(s) 1067, 1788
BciVI GTATCC 1 cut(s) 2122
BcoDI GTCTC 2 cut(s) 521, 1816
BcuI ACTAGT 2 cut(s) 574, 922
BfaI CTAG 6 cut(s) 575, 645, 923, 1001, 1209, 1938
BfmI CTRYAG 4 cut(s) 301, 1248, 1555, 1756
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 3 cut(s) 308, 802, 937
BfuI GTATCC 1 cut(s) 2122
BglII AGATCT 2 cut(s) 553, 658
BisI GCNGC 7 cut(s) 159, 361, 428, 1044, 1105, 1582, 1864
BlsI GCNGC 7 cut(s) 160, 362, 429, 1045, 1106, 1583, 1865
Bme1390I CCNGG 2 cut(s) 1067, 1788
Bme18I GGWCC 2 cut(s) 1945, 2231
BmeT110I CYCGRG 1 cut(s) 1441
BmgT120I GGNCC 3 cut(s) 1031, 1945, 2231
BmiI GGNNCC 7 cut(s) 342, 814, 1601, 1750, 1793, 1842, 1947
BmrFI CCNGG 2 cut(s) 1067, 1788
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 8 cut(s) 44, 170, 720, 985, 1287, 1528, 1717, 1888
BmuI ACTGGG 1 cut(s) 864
BpiI GAAGAC 2 cut(s) 635, 1320
BplI GAGNNNNNCTC 2 cut(s) 1934, 1966
BpmI CTGGAG 2 cut(s) 1576, 2004
BpuEI CTTGAG 1 cut(s) 397
BsaBI GATNNNNATC 2 cut(s) 657, 1854
BsaHI GRCGYC 1 cut(s) 1695
BsaJI CCNNGG 3 cut(s) 1787, 1980, 2255
BsaWI WCCGGW 1 cut(s) 565
Bsc4I CCNNNNNNNGG 5 cut(s) 406, 538, 599, 1115, 1775
Bse1I ACTGG 6 cut(s) 400, 482, 859, 1769, 2054, 2285
Bse3DI GCAATG 2 cut(s) 740, 1226
Bse8I GATNNNNATC 2 cut(s) 657, 1854
BseBI CCWGG 2 cut(s) 1067, 1788
BseDI CCNNGG 3 cut(s) 1787, 1980, 2255
BseGI GGATG 6 cut(s) 10, 35, 161, 735, 2014, 2182
BseJI GATNNNNATC 2 cut(s) 657, 1854
BseLI CCNNNNNNNGG 5 cut(s) 406, 538, 599, 1115, 1775
BseMI GCAATG 2 cut(s) 740, 1226
BseMII CTCAG 4 cut(s) 6, 86, 629, 1988
BseNI ACTGG 6 cut(s) 400, 482, 859, 1769, 2054, 2285
BseRI GAGGAG 1 cut(s) 2154
BseSI GKGCMC 1 cut(s) 1159
BseXI GCAGC 7 cut(s) 170, 347, 439, 1055, 1091, 1593, 1875
BseYI CCCAGC 2 cut(s) 76, 1682
BsgI GTGCAG 2 cut(s) 446, 780
BshFI GGCC 4 cut(s) 490, 739, 1033, 2010
BshNI GGYRCC 1 cut(s) 812
BsiHKCI CYCGRG 1 cut(s) 1441
BsiSI CCGG 1 cut(s) 566
BslFI GGGAC 1 cut(s) 2057
BslI CCNNNNNNNGG 5 cut(s) 406, 538, 599, 1115, 1775
BsmAI GTCTC 2 cut(s) 521, 1816
BsmBI CGTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 2057
BsmI GAATGC 1 cut(s) 1693
BsnI GGCC 4 cut(s) 490, 739, 1033, 2010
BsoBI CYCGRG 1 cut(s) 1441
Bsp1286I GDGCHC 1 cut(s) 1159
BspACI CCGC 4 cut(s) 1026, 1102, 1596, 1847
BspANI GGCC 4 cut(s) 490, 739, 1033, 2010
BspCNI CTCAG 4 cut(s) 7, 85, 628, 1987
BspLI GGNNCC 7 cut(s) 342, 814, 1601, 1750, 1793, 1842, 1947
BspMAI CTGCAG 1 cut(s) 305
BspMI ACCTGC 3 cut(s) 308, 802, 937
BspT107I GGYRCC 1 cut(s) 812
BspTI CTTAAG 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 740, 1226
BsrI ACTGG 6 cut(s) 400, 482, 859, 1769, 2054, 2285
BssECI CCNNGG 3 cut(s) 1787, 1980, 2255
BssNAI GTATAC 1 cut(s) 701
BssNI GRCGYC 1 cut(s) 1695
BssT1I CCWWGG 1 cut(s) 2255
Bst1107I GTATAC 1 cut(s) 701
Bst2UI CCWGG 2 cut(s) 1067, 1788
Bst4CI ACNGT 9 cut(s) 503, 510, 547, 744, 784, 940, 1271, 1760, 2130
Bst6I CTCTTC 3 cut(s) 1885, 1989, 2064
BstACI GRCGYC 1 cut(s) 1695
BstAFI CTTAAG 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 1035, 1138, 2098
BstDEI CTNAG 7 cut(s) 15, 72, 124, 615, 1534, 1706, 1974
BstF5I GGATG 6 cut(s) 10, 35, 161, 735, 2014, 2182
BstHHI GCGC 1 cut(s) 1390
BstMAI GTCTC 2 cut(s) 521, 1816
BstMWI GCNNNNNNNGC 4 cut(s) 167, 269, 1043, 1688
BstNI CCWGG 2 cut(s) 1067, 1788
BstNSI RCATGY 3 cut(s) 85, 891, 2111
BstSCI CCNGG 2 cut(s) 1065, 1786
BstSFI CTRYAG 4 cut(s) 301, 1248, 1555, 1756
BstSLI GKGCMC 1 cut(s) 1159
BstV1I GCAGC 7 cut(s) 170, 347, 439, 1055, 1091, 1593, 1875
BstV2I GAAGAC 2 cut(s) 635, 1320
BstX2I RGATCY 8 cut(s) 106, 340, 553, 658, 1409, 1783, 1849, 2052
BstXI CCANNNNNNTGG 1 cut(s) 1802
BstYI RGATCY 8 cut(s) 106, 340, 553, 658, 1409, 1783, 1849, 2052
BstZ17I GTATAC 1 cut(s) 701
BsuI GTATCC 1 cut(s) 2122
BsuRI GGCC 4 cut(s) 490, 739, 1033, 2010
BtsCI GGATG 6 cut(s) 10, 35, 161, 735, 2014, 2182
BtsI GCAGTG 4 cut(s) 310, 1397, 1591, 1952
BtsIMutI CAGTG 7 cut(s) 310, 407, 789, 852, 1397, 1591, 1952
BveI ACCTGC 3 cut(s) 308, 802, 937
Cac8I GCNNGC 3 cut(s) 1035, 1138, 2098
CaiI CAGNNNCTG 2 cut(s) 109, 1787
CfoI GCGC 1 cut(s) 1390
Cfr13I GGNCC 3 cut(s) 1031, 1945, 2231
Csp6I GTAC 2 cut(s) 1761, 2126
CspCI CAANNNNNGTGG 2 cut(s) 1148, 1183
CviAII CATG 7 cut(s) 82, 888, 1162, 1416, 1892, 2108, 2120
CviQI GTAC 2 cut(s) 1761, 2126
DdeI CTNAG 7 cut(s) 15, 72, 124, 615, 1534, 1706, 1974
DraI TTTAAA 1 cut(s) 1470
DraIII CACNNNGTG 1 cut(s) 1567
EaeI YGGCCR 2 cut(s) 737, 2008
Eam1104I CTCTTC 3 cut(s) 1885, 1989, 2064
EarI CTCTTC 3 cut(s) 1885, 1989, 2064
Eco130I CCWWGG 1 cut(s) 2255
Eco47I GGWCC 2 cut(s) 1945, 2231
Eco57I CTGAAG 4 cut(s) 1068, 1256, 1527, 1698
Eco88I CYCGRG 1 cut(s) 1441
EcoO109I RGGNCCY 1 cut(s) 1945
EcoRI GAATTC 1 cut(s) 1008
EcoRII CCWGG 2 cut(s) 1065, 1786
EcoT14I CCWWGG 1 cut(s) 2255
ErhI CCWWGG 1 cut(s) 2255
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 7 cut(s) 85, 891, 1165, 1419, 1895, 2111, 2123
FaqI GGGAC 1 cut(s) 2057
FatI CATG 7 cut(s) 81, 887, 1161, 1415, 1891, 2107, 2119
FauI CCCGC 1 cut(s) 1589
FblI GTMKAC 1 cut(s) 700
Fnu4HI GCNGC 7 cut(s) 159, 361, 428, 1044, 1105, 1582, 1864
FokI GGATG 6 cut(s) 17, 22, 148, 742, 2001, 2189
Fsp4HI GCNGC 7 cut(s) 159, 361, 428, 1044, 1105, 1582, 1864
FspBI CTAG 6 cut(s) 575, 645, 923, 1001, 1209, 1938
FspI TGCGCA 1 cut(s) 1389
GlaI GCGC 1 cut(s) 1389
GluI GCNGC 7 cut(s) 159, 361, 428, 1044, 1105, 1582, 1864
GsaI CCCAGC 2 cut(s) 80, 1686
GsuI CTGGAG 2 cut(s) 1576, 2004
HaeIII GGCC 4 cut(s) 490, 739, 1033, 2010
HapII CCGG 1 cut(s) 566
HhaI GCGC 1 cut(s) 1390
Hin1I GRCGYC 1 cut(s) 1695
Hin1II CATG 7 cut(s) 85, 891, 1165, 1419, 1895, 2111, 2123
Hin6I GCGC 1 cut(s) 1388
HinP1I GCGC 1 cut(s) 1388
HindIII AAGCTT 1 cut(s) 231
HpaII CCGG 1 cut(s) 566
HphI GGTGA 6 cut(s) 580, 992, 1382, 1391, 1433, 1679
Hpy166II GTNNAC 3 cut(s) 409, 506, 701
Hpy188III TCNNGA 7 cut(s) 344, 455, 645, 824, 1178, 1746, 2021
Hpy8I GTNNAC 3 cut(s) 409, 506, 701
Hpy99I CGWCG 2 cut(s) 936, 1017
HpyAV CCTTC 1 cut(s) 1123
HpyCH4III ACNGT 9 cut(s) 503, 510, 547, 744, 784, 940, 1271, 1760, 2130
HpyCH4IV ACGT 4 cut(s) 63, 313, 1257, 1695
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 269, 1043, 1688
HpyF3I CTNAG 7 cut(s) 15, 72, 124, 615, 1534, 1706, 1974
HpySE526I ACGT 4 cut(s) 63, 313, 1257, 1695
Hsp92I GRCGYC 1 cut(s) 1695
Hsp92II CATG 7 cut(s) 85, 891, 1165, 1419, 1895, 2111, 2123
HspAI GCGC 1 cut(s) 1388
LmnI GCTCC 1 cut(s) 1192
Lsp1109I GCAGC 7 cut(s) 170, 347, 439, 1055, 1091, 1593, 1875
LweI GCATC 8 cut(s) 44, 170, 720, 985, 1287, 1528, 1717, 1888
MaeI CTAG 6 cut(s) 575, 645, 923, 1001, 1209, 1938
MaeII ACGT 4 cut(s) 63, 313, 1257, 1695
MaeIII GTNAC 8 cut(s) 577, 1061, 1501, 1559, 1586, 1709, 2135, 2211
MfeI CAATTG 2 cut(s) 594, 803
MflI RGATCY 8 cut(s) 106, 340, 553, 658, 1409, 1783, 1849, 2052
MhlI GDGCHC 1 cut(s) 1159
MlsI TGGCCA 2 cut(s) 739, 2010
MluNI TGGCCA 2 cut(s) 739, 2010
MlyI GAGTC 6 cut(s) 54, 64, 404, 1076, 1583, 1706
MmeI TCCRAC 3 cut(s) 1670, 1971, 1994
Mox20I TGGCCA 2 cut(s) 739, 2010
MroXI GAANNNNTTC 1 cut(s) 2085
MscI TGGCCA 2 cut(s) 739, 2010
MseI TTAA 7 cut(s) 57, 327, 336, 708, 839, 1469, 2172
MslI CAYNNNNRTG 1 cut(s) 1896
Msp20I TGGCCA 2 cut(s) 739, 2010
MspA1I CMGCKG 6 cut(s) 76, 263, 1046, 1104, 1596, 1847
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 1 cut(s) 566
MspR9I CCNGG 2 cut(s) 1067, 1788
MssI GTTTAAAC 1 cut(s) 1470
MunI CAATTG 2 cut(s) 594, 803
Mva1269I GAATGC 1 cut(s) 1693
MvaI CCWGG 2 cut(s) 1067, 1788
MwoI GCNNNNNNNGC 4 cut(s) 167, 269, 1043, 1688
NlaIII CATG 7 cut(s) 85, 891, 1165, 1419, 1895, 2111, 2123
NlaIV GGNNCC 7 cut(s) 342, 814, 1601, 1750, 1793, 1842, 1947
NmeAIII GCCGAG 1 cut(s) 1961
NmuCI GTSAC 5 cut(s) 1061, 1559, 1586, 1709, 2211
NsbI TGCGCA 1 cut(s) 1389
NspI RCATGY 3 cut(s) 85, 891, 2111
PaeR7I CTCGAG 1 cut(s) 1441
PaqCI CACCTGC 1 cut(s) 308
PciI ACATGT 1 cut(s) 2107
PcsI WCGNNNNNNNCGW 2 cut(s) 520, 1012
PctI GAATGC 1 cut(s) 1693
PdmI GAANNNNTTC 1 cut(s) 2085
PflMI CCANNNNNTGG 3 cut(s) 406, 599, 1775
PkrI GCNGC 7 cut(s) 160, 362, 429, 1045, 1106, 1583, 1865
PleI GAGTC 6 cut(s) 53, 64, 404, 1076, 1583, 1706
PmeI GTTTAAAC 1 cut(s) 1470
PpsI GAGTC 6 cut(s) 53, 64, 404, 1076, 1583, 1706
PpuMI RGGWCCY 1 cut(s) 1945
PscI ACATGT 1 cut(s) 2107
Psp5II RGGWCCY 1 cut(s) 1945
Psp6I CCWGG 2 cut(s) 1065, 1786
PspFI CCCAGC 2 cut(s) 76, 1682
PspGI CCWGG 2 cut(s) 1065, 1786
PspN4I GGNNCC 7 cut(s) 342, 814, 1601, 1750, 1793, 1842, 1947
PspPI GGNCC 3 cut(s) 1031, 1945, 2231
PspPPI RGGWCCY 1 cut(s) 1945
PspXI VCTCGAGB 1 cut(s) 1441
PstI CTGCAG 1 cut(s) 305
PstNI CAGNNNCTG 2 cut(s) 109, 1787
PsuI RGATCY 8 cut(s) 106, 340, 553, 658, 1409, 1783, 1849, 2052
PvuII CAGCTG 3 cut(s) 76, 263, 1046
RsaI GTAC 2 cut(s) 1762, 2127
RsaNI GTAC 2 cut(s) 1761, 2126
RseI CAYNNNNRTG 1 cut(s) 1896
SaqAI TTAA 7 cut(s) 57, 327, 336, 708, 839, 1469, 2172
SatI GCNGC 7 cut(s) 159, 361, 428, 1044, 1105, 1582, 1864
Sau96I GGNCC 3 cut(s) 1031, 1945, 2231
SchI GAGTC 6 cut(s) 54, 64, 404, 1076, 1583, 1706
ScrFI CCNGG 2 cut(s) 1067, 1788
SduI GDGCHC 1 cut(s) 1159
SfaNI GCATC 8 cut(s) 44, 170, 720, 985, 1287, 1528, 1717, 1888
SfcI CTRYAG 4 cut(s) 301, 1248, 1555, 1756
Sfr274I CTCGAG 1 cut(s) 1441
SinI GGWCC 2 cut(s) 1945, 2231
SlaI CTCGAG 1 cut(s) 1441
SmiMI CAYNNNNRTG 1 cut(s) 1896
SmlI CTYRAG 3 cut(s) 56, 412, 1441
SmoI CTYRAG 3 cut(s) 56, 412, 1441
SpeI ACTAGT 2 cut(s) 574, 922
SsiI CCGC 4 cut(s) 1026, 1102, 1596, 1847
SspI AATATT 2 cut(s) 589, 1799
SspMI CTAG 6 cut(s) 575, 645, 923, 1001, 1209, 1938
StyD4I CCNGG 2 cut(s) 1065, 1786
StyI CCWWGG 1 cut(s) 2255
TaaI ACNGT 9 cut(s) 503, 510, 547, 744, 784, 940, 1271, 1760, 2130
TaiI ACGT 4 cut(s) 66, 316, 1260, 1698
TaqI TCGA 5 cut(s) 43, 182, 1123, 1442, 2062
TatI WGTACW 2 cut(s) 1760, 2125
Tru1I TTAA 7 cut(s) 57, 327, 336, 708, 839, 1469, 2172
Tru9I TTAA 7 cut(s) 57, 327, 336, 708, 839, 1469, 2172
TscAI CASTG 7 cut(s) 310, 407, 789, 859, 1397, 1591, 1959
TseFI GTSAC 5 cut(s) 1061, 1559, 1586, 1709, 2211
TseI GCWGC 7 cut(s) 158, 360, 427, 1043, 1104, 1581, 1863
Tsp45I GTSAC 5 cut(s) 1061, 1559, 1586, 1709, 2211
TspGWI ACGGA 2 cut(s) 2230, 2244
TspRI CASTG 7 cut(s) 310, 407, 789, 859, 1397, 1591, 1959
Van91I CCANNNNNTGG 3 cut(s) 406, 599, 1775
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 2 cut(s) 1945, 2231
XapI RAATTY 3 cut(s) 1008, 1960, 2219
XbaI TCTAGA 1 cut(s) 644
XceI RCATGY 3 cut(s) 85, 891, 2111
XcmI CCANNNNNNNNNTGG 2 cut(s) 1347, 1898
XhoI CTCGAG 1 cut(s) 1441
XmiI GTMKAC 1 cut(s) 700
XmnI GAANNNNTTC 1 cut(s) 2085
XspI CTAG 6 cut(s) 575, 645, 923, 1001, 1209, 1938
ZraI GACGTC 1 cut(s) 1696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.