Rw1G028290

MIZ/SP-RING zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000955
Physical Location & Seq
Reverse (-)
55957383 .. 55966680
9298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028290.1

Sequence Viewer

Length: 2583 bp
ATGGATTTGGTAACTCAATGCAAGACCAAGTTGGTGTCTTTTCGAATTAAGGAGCTCAAGGATGTTCTGACCCAGCTGGGTTTTTCAAAGCAAGGGAGGAAGCAGGATCTAATTGACAGAATCTTAGCATCAGTTTCGGACGAAGAAACATCCAATACACGCAGTTTGTCAAAGAAAAAACACATCGAGAAGAATGGAGTAGTGCAAGTAATTGATGAAGCTTACAGAAAGATGCAGAGTACACCGTCAACGGATTCTCCGACAAAGGAACAAAGTGGTTCAGATGGCTGCAGTGTGAAGCCCTCTAGAAAGGAAGTTAAAAATTTGAATGTGACAGACGTGAAGATTTCTTGCCCCTGTAAAAGTTCATTGTCAGCTGATTCAATGATACAGTGTGTAAATCCAATATGTCAAGTTCAGCAACATATTGGTTGCGTGATTATTCCTGAAAAAACAAAGGATTGCAGTCCACCAGTTCCGCCTCTTTTCTACTGTGAAATGTGTCGTCTCAAACGAGCAGATCCTTACTGGGTGACTGTGGTGGATCTAGTATCTGCAGTGAAGTTAACTGCTTCAAATATTTCAATTGATGGTGCAAATCCAACTCAGAATGTGGAGAAAAATTTTCAACTTTCCAGAGCCAATAGAGATCTACTTCATCATAATGAATATGATGTTCAGGCTTGGTGTATACTTCTCAATGATAGTGTTTCATTTAGGTTGCAATGGCCTCAGTTTGCAGAATTACAAGTTAATGGTACGATTGTTAGGGCAGTTAATAGACCTGGACATCAATTGTTAGGTGCTAATGGCCGTGATGATGGAGCATTAATCAAATTTTGCATTGGGGAGGGAATCAATAAGATTTCATTATCAGGATGTGATAATCGTGCTTTCTGTTTTGGCGTCAGACTGGTAAAACGACGAACATTTCAGCAGGTTCTCAACCTCATTCCTAAAGAAGAAGATGGTGAGCTTTTTGAGGATGCACTTGCGCGGGTTCGTCGTTGCATTGGTGGTGGGGCTGCTATGGCACATGAAGATAGTGATAGTGATTTAGAAGTGATTTCAGATTCAGTTTCCATGAGTGGTTCTCGAATGAAGGTAGCTGGCAGATTCAAACCTTGTGCTCACATGGGTTGCTTTGATCTCGAAACTTTCGTGGAGCTAAACCAGCGATCTAGGAAGTGGCAATGCCCCATATGCCTCAAGAACTACTCGTTGGAGGACATCATCATTGACCGTTATTTCAATCGCATCACAACTATGATGCAAATTTGTGGGGAAGACATAACAGAGATCAATGTGAAGCCAGATGGTTCTTGGAGTGCAAAGACTAAAGGTGAGTTTAGCGACCTTGCGCAATGGCACTTTCCTGATGGATCTCCATATGTTGGTATGGATTTGGGAACCACAGGCCAGTCCAACCCAGAATCATGTTCAAACCAACACCATAGGTTTATGTCTAATCATGGTGGGGTCAATGAAGTTGGTAAACATCTGGGTGATCCATTAGACAAAGAATTTGAGGCTTTCGAGCAAAAGGTTATAACAATGAGCAGCAGTGACACTTCCTGTGGCAGAGAGGATGAAGACTTTATTATGAATCAAAAAGGCAGTGTTCATATAGATGTCTCCGCATATGATGACAACGAAGTCAATTCACTTTCTCCTAATTTTGCCTCAACATTTGAAATTGGGAATGGTACTTCTGCTGAAGCTGTTAATACTGGTATTATTATTCTCAGTGACTCGGAAGATGAAACAGTCAACTTGGTTTCTCCAAGAGTTATCAACAATACTTGCCCAGTAAGCAGCAGTGCATGCTCCCTGTCTGCAGCTCCTGGCATTTCAGACCCATATGCGTCTTGTTTGGGTCTTTTAAATGGAAATGGTAATGACACTGGGATGTCACAATATGCATATCCTACTGGTACTCACGCCGATCCTGGGTTCCAATTATTTGGTACTGATTCAGATCTCTCCGATGCTTTTATTGATTTGGAGCAGACATCAGTTGATGGTTCAGCTCCAATGAATGGTAACACATTGGTTTCAGATAAAAATCTAGAACCAAGCGGACATGTCGTAAACTCTTCTGTTTCCCATACCAATGCTAACTTGGCTAGCTTGGTTGATAATCCTTTGGGCTTTGGTAGTGAGGACCCCTCAATGCAAAGTTTTCTTCCAAGTCATCCCTCTTGTTTGTTGGAGCAGTCTCATTTTGGACAACATCAACCAGTCCCAAATGGCATTACTAGTGATTGGGTTGCTCTTAGCCTTGGTATAGGTGAAAGTGTTCCCAATGACGTTGATGCAAATGCTGAACCTGCAGACACAAATGGACTATATTCAAGAAACCAATGTGGATCAAATAAGGTGGCTTTAATCACGGATAAAAATGAGGGATCTAAGTCGAAATCTAACAGAGCAAATATAAGGAAACTTTCAGATGGACCTTTCTCCTTTCCCCGTCAGCCACGGTCTATATCTTTGATATGCAGCATCTTTATTGCTCCCCACCGACCATTGGTATTCTTTTGGCTGTGTTCTTTCCCAATTGTTTCTTTCTTCATCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04630 ko05160 map04120 map04630 map05160
Pfam Domains
Protein Families
SAP zf-MIZ

Protein Analysis

860

Amino Acids

94.05

Weight (kDa)

5.27

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP PF02037 11 - 42 3.7e-07 SAP domain
zf-MIZ PF02891 362 - 404 1.5e-15 MIZ/SP-RING zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000955)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970
malus_domestica MD01G1111200.v1.1 MD07G1177400.v1.1
prunus_persica Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1
pyrus_communis pycom01g13860 pycom07g17140
rosa_chinensis RchiOBHm_Chr1g0363931
rosa_laevigata RLG00000027534
rosa_multiflora Rmu_sc0001607.1_g000006
rosa_roxburghii Rroxscaffold_4G00292830
rosa_rugosa Rorug01G0311200 Rorug01G0311300
rosa_samantha Rh1AG319200 Rh1BG282200 Rh1CG298700 Rh1DG313600
rosa_wichuraiana Rw1G028290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1550
AasI GACNNNNNNGTC 1 cut(s) 1655
Acc16I TGCGCA 1 cut(s) 1362
Acc36I ACCTGC 2 cut(s) 928, 2338
AccB7I CCANNNNNTGG 2 cut(s) 1394, 2039
AccI GTMKAC 1 cut(s) 691
AccII CGCG 1 cut(s) 997
AciI CCGC 4 cut(s) 479, 997, 1638, 2079
AclWI GGATC 8 cut(s) 114, 515, 552, 1390, 1502, 1940, 2377, 2416
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 5 cut(s) 322, 622, 836, 1275, 1523
AcuI CTGAAG 1 cut(s) 1737
AcyI GRCGYC 1 cut(s) 906
AfaI GTAC 5 cut(s) 241, 760, 1708, 1936, 1969
AfiI CCNNNNNNNGG 4 cut(s) 1394, 1950, 2039, 2530
AflIII ACRYGT 1 cut(s) 2083
AhlI ACTAGT 1 cut(s) 2258
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 3 cut(s) 784, 1843, 1948
AjuI GAANNNNNNNTTGG 2 cut(s) 1205, 1237
Alw21I GWGCWC 2 cut(s) 57, 1132
Alw26I GTCTC 3 cut(s) 512, 1639, 2223
AlwI GGATC 8 cut(s) 114, 515, 552, 1390, 1502, 1940, 2377, 2416
AlwNI CAGNNNCTG 1 cut(s) 1844
AoxI GGCC 3 cut(s) 728, 811, 1417
ApeKI GCWGC 6 cut(s) 288, 1025, 1560, 1815, 1838, 2502
ApoI RAATTY 5 cut(s) 322, 622, 836, 1275, 1523
ArsI GACNNNNNNTTYG 2 cut(s) 1508, 1540
AseI ATTAAT 1 cut(s) 830
Asp700I GAANNNNTTC 1 cut(s) 2298
AspLEI GCGC 2 cut(s) 997, 1363
AspS9I GGNCC 2 cut(s) 2164, 2456
AsuHPI GGTGA 5 cut(s) 544, 983, 1355, 1517, 2303
AsuII TTCGAA 1 cut(s) 43
AsuNHI GCTAGC 1 cut(s) 2126
AvaII GGWCC 2 cut(s) 2164, 2456
BaeI ACNNNNGTAYC 2 cut(s) 750, 783
BanII GRGCYC 1 cut(s) 57
BbsI GAAGAC 2 cut(s) 1293, 1599
Bbv12I GWGCWC 2 cut(s) 57, 1132
BbvI GCAGC 6 cut(s) 275, 1012, 1572, 1827, 1850, 2514
BccI CCATC 8 cut(s) 278, 584, 815, 962, 1310, 1373, 2015, 2447
BceAI ACGGC 1 cut(s) 798
BcgI CGANNNNNNTGC 2 cut(s) 117, 151
BciT130I CCWGG 3 cut(s) 786, 1845, 1950
BcoDI GTCTC 3 cut(s) 512, 1639, 2223
BcuI ACTAGT 1 cut(s) 2258
BfaI CTAG 6 cut(s) 306, 548, 1182, 2069, 2127, 2259
BfmI CTRYAG 4 cut(s) 289, 555, 1836, 2331
BfuAI ACCTGC 2 cut(s) 928, 2338
BglII AGATCT 2 cut(s) 649, 1978
BisI GCNGC 6 cut(s) 289, 1026, 1561, 1816, 1839, 2503
BlsI GCNGC 6 cut(s) 290, 1027, 1562, 1817, 1840, 2504
Bme1390I CCNGG 3 cut(s) 786, 1845, 1950
Bme18I GGWCC 2 cut(s) 2164, 2456
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 2164, 2456
BmiI GGNNCC 3 cut(s) 1411, 1955, 2166
BmrFI CCNGG 3 cut(s) 786, 1845, 1950
BmrI ACTGGG 3 cut(s) 538, 1802, 1914
BmsI GCATC 8 cut(s) 137, 222, 976, 1260, 1266, 1978, 2305, 2514
BmtI GCTAGC 1 cut(s) 2130
BmuI ACTGGG 3 cut(s) 538, 1802, 1914
BpiI GAAGAC 2 cut(s) 1293, 1599
BplI GAGNNNNNCTC 4 cut(s) 1078, 1110, 2153, 2185
Bpu14I TTCGAA 1 cut(s) 43
BpuEI CTTGAG 2 cut(s) 41, 1193
BsaBI GATNNNNATC 2 cut(s) 1977, 2064
BsaHI GRCGYC 1 cut(s) 906
BsaJI CCNNGG 3 cut(s) 1949, 2281, 2480
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 4 cut(s) 1394, 1950, 2039, 2530
Bse1I ACTGG 9 cut(s) 473, 533, 918, 1420, 1735, 1808, 1909, 1936, 2240
Bse3DI GCAATG 3 cut(s) 731, 1199, 1370
Bse8I GATNNNNATC 2 cut(s) 1977, 2064
BseBI CCWGG 3 cut(s) 786, 1845, 1950
BseDI CCNNGG 3 cut(s) 1949, 2281, 2480
BseGI GGATG 7 cut(s) 67, 149, 884, 991, 1594, 1914, 2194
BseJI GATNNNNATC 2 cut(s) 1977, 2064
BseLI CCNNNNNNNGG 4 cut(s) 1394, 1950, 2039, 2530
BseMI GCAATG 3 cut(s) 731, 1199, 1370
BseMII CTCAG 3 cut(s) 620, 746, 1759
BseNI ACTGG 9 cut(s) 473, 533, 918, 1420, 1735, 1808, 1909, 1936, 2240
BseXI GCAGC 6 cut(s) 275, 1012, 1572, 1827, 1850, 2514
BseYI CCCAGC 2 cut(s) 72, 76
Bsh1236I CGCG 1 cut(s) 997
BshFI GGCC 3 cut(s) 730, 813, 1419
BsiHKAI GWGCWC 2 cut(s) 57, 1132
BslFI GGGAC 1 cut(s) 2228
BslI CCNNNNNNNGG 4 cut(s) 1394, 1950, 2039, 2530
BsmAI GTCTC 3 cut(s) 512, 1639, 2223
BsmBI CGTCTC 1 cut(s) 512
BsmFI GGGAC 1 cut(s) 2228
BsnI GGCC 3 cut(s) 730, 813, 1419
Bsp119I TTCGAA 1 cut(s) 43
Bsp1286I GDGCHC 2 cut(s) 57, 1132
BspACI CCGC 4 cut(s) 479, 997, 1638, 2079
BspANI GGCC 3 cut(s) 730, 813, 1419
BspCNI CTCAG 3 cut(s) 619, 745, 1758
BspFNI CGCG 1 cut(s) 997
BspLI GGNNCC 3 cut(s) 1411, 1955, 2166
BspMAI CTGCAG 4 cut(s) 293, 559, 1840, 2335
BspMI ACCTGC 2 cut(s) 928, 2338
BspOI GCTAGC 1 cut(s) 2130
BspPI GGATC 8 cut(s) 114, 515, 552, 1390, 1502, 1940, 2377, 2416
BspT104I TTCGAA 1 cut(s) 43
BsrDI GCAATG 3 cut(s) 731, 1199, 1370
BsrI ACTGG 9 cut(s) 473, 533, 918, 1420, 1735, 1808, 1909, 1936, 2240
BssECI CCNNGG 3 cut(s) 1949, 2281, 2480
BssNAI GTATAC 1 cut(s) 692
BssNI GRCGYC 1 cut(s) 906
BssT1I CCWWGG 1 cut(s) 2281
Bst1107I GTATAC 1 cut(s) 692
Bst2UI CCWGG 3 cut(s) 786, 1845, 1950
Bst4CI ACNGT 7 cut(s) 246, 393, 494, 538, 1244, 1768, 2484
Bst6I CTCTTC 1 cut(s) 2101
BstACI GRCGYC 1 cut(s) 906
BstAPI GCANNNNNTGC 1 cut(s) 1824
BstBI TTCGAA 1 cut(s) 43
BstC8I GCNNGC 3 cut(s) 1111, 1825, 2128
BstDEI CTNAG 6 cut(s) 124, 606, 732, 1745, 2276, 2412
BstDSI CCRYGG 1 cut(s) 2480
BstF5I GGATG 7 cut(s) 67, 149, 884, 991, 1594, 1914, 2194
BstFNI CGCG 1 cut(s) 997
BstHHI GCGC 2 cut(s) 997, 1363
BstMAI GTCTC 3 cut(s) 512, 1639, 2223
BstMWI GCNNNNNNNGC 7 cut(s) 1031, 1174, 1203, 1812, 1824, 2123, 2330
BstNI CCWGG 3 cut(s) 786, 1845, 1950
BstNSI RCATGY 2 cut(s) 1827, 2087
BstSCI CCNGG 3 cut(s) 784, 1843, 1948
BstSFI CTRYAG 4 cut(s) 289, 555, 1836, 2331
BstUI CGCG 1 cut(s) 997
BstV1I GCAGC 6 cut(s) 275, 1012, 1572, 1827, 1850, 2514
BstV2I GAAGAC 2 cut(s) 1293, 1599
BstX2I RGATCY 7 cut(s) 106, 520, 544, 649, 1382, 1978, 2408
BstXI CCANNNNNNTGG 1 cut(s) 1964
BstYI RGATCY 7 cut(s) 106, 520, 544, 649, 1382, 1978, 2408
BstZ17I GTATAC 1 cut(s) 692
BsuRI GGCC 3 cut(s) 730, 813, 1419
BtgI CCRYGG 1 cut(s) 2480
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 7 cut(s) 67, 149, 884, 991, 1594, 1914, 2194
BtsI GCAGTG 5 cut(s) 298, 564, 1570, 1624, 1825
BtsIMutI CAGTG 8 cut(s) 298, 398, 564, 1570, 1624, 1753, 1825, 1902
BveI ACCTGC 2 cut(s) 928, 2338
Cac8I GCNNGC 3 cut(s) 1111, 1825, 2128
CaiI CAGNNNCTG 1 cut(s) 1844
CfoI GCGC 2 cut(s) 997, 1363
Cfr13I GGNCC 2 cut(s) 2164, 2456
CseI GACGC 2 cut(s) 895, 1854
Csp6I GTAC 5 cut(s) 240, 759, 1707, 1935, 1968
CspCI CAANNNNNGTGG 2 cut(s) 2361, 2396
CviAII CATG 7 cut(s) 1037, 1084, 1135, 1437, 1472, 1824, 2084
CviQI GTAC 5 cut(s) 240, 759, 1707, 1935, 1968
DdeI CTNAG 6 cut(s) 124, 606, 732, 1745, 2276, 2412
DraI TTTAAA 1 cut(s) 1884
DrdI GACNNNNNNGTC 1 cut(s) 1655
DseDI GACNNNNNNGTC 1 cut(s) 1655
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 2101
EarI CTCTTC 1 cut(s) 2101
EciI GGCGGA 1 cut(s) 468
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 2281
Eco24I GRGCYC 1 cut(s) 57
Eco47I GGWCC 2 cut(s) 2164, 2456
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 1737
EcoICRI GAGCTC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 2164
EcoRII CCWGG 3 cut(s) 784, 1843, 1948
EcoT14I CCWWGG 1 cut(s) 2281
EcoT22I ATGCAT 1 cut(s) 1924
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 2281
Esp3I CGTCTC 1 cut(s) 512
FaeI CATG 7 cut(s) 1040, 1087, 1138, 1440, 1475, 1827, 2087
FaqI GGGAC 1 cut(s) 2228
FatI CATG 7 cut(s) 1036, 1083, 1134, 1436, 1471, 1823, 2083
FauI CCCGC 1 cut(s) 990
FauNDI CATATG 4 cut(s) 1202, 1390, 1642, 1861
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 6 cut(s) 289, 1026, 1561, 1816, 1839, 2503
FokI GGATG 7 cut(s) 74, 136, 891, 998, 1601, 1921, 2181
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 6 cut(s) 289, 1026, 1561, 1816, 1839, 2503
FspBI CTAG 6 cut(s) 306, 548, 1182, 2069, 2127, 2259
FspI TGCGCA 1 cut(s) 1362
GlaI GCGC 2 cut(s) 996, 1362
GluI GCNGC 6 cut(s) 289, 1026, 1561, 1816, 1839, 2503
GsaI CCCAGC 2 cut(s) 76, 80
HaeIII GGCC 3 cut(s) 730, 813, 1419
HgaI GACGC 2 cut(s) 895, 1854
HhaI GCGC 2 cut(s) 997, 1363
Hin1I GRCGYC 1 cut(s) 906
Hin1II CATG 7 cut(s) 1040, 1087, 1138, 1440, 1475, 1827, 2087
Hin6I GCGC 2 cut(s) 995, 1361
HinP1I GCGC 2 cut(s) 995, 1361
HincII GTYRAC 3 cut(s) 249, 567, 1771
HindII GTYRAC 3 cut(s) 249, 567, 1771
HindIII AAGCTT 1 cut(s) 219
HpaI GTTAAC 1 cut(s) 567
HphI GGTGA 5 cut(s) 544, 983, 1355, 1517, 2303
Hpy166II GTNNAC 8 cut(s) 242, 249, 470, 567, 692, 1496, 1771, 2092
Hpy8I GTNNAC 8 cut(s) 242, 249, 470, 567, 692, 1496, 1771, 2092
Hpy99I CGWCG 2 cut(s) 927, 1008
HpyAV CCTTC 1 cut(s) 1096
HpyCH4III ACNGT 7 cut(s) 246, 393, 494, 538, 1244, 1768, 2484
HpyCH4IV ACGT 2 cut(s) 339, 2310
HpyF10VI GCNNNNNNNGC 7 cut(s) 1031, 1174, 1203, 1812, 1824, 2123, 2330
HpyF3I CTNAG 6 cut(s) 124, 606, 732, 1745, 2276, 2412
HpySE526I ACGT 2 cut(s) 339, 2310
Hsp92I GRCGYC 1 cut(s) 906
Hsp92II CATG 7 cut(s) 1040, 1087, 1138, 1440, 1475, 1827, 2087
HspAI GCGC 2 cut(s) 995, 1361
KspAI GTTAAC 1 cut(s) 567
LmnI GCTCC 9 cut(s) 52, 824, 1165, 1832, 1846, 2005, 2035, 2212, 2521
Lsp1109I GCAGC 6 cut(s) 275, 1012, 1572, 1827, 1850, 2514
LweI GCATC 8 cut(s) 137, 222, 976, 1260, 1266, 1978, 2305, 2514
MaeI CTAG 6 cut(s) 306, 548, 1182, 2069, 2127, 2259
MaeII ACGT 2 cut(s) 339, 2310
MaeIII GTNAC 7 cut(s) 10, 331, 532, 1565, 1748, 1911, 2042
MfeI CAATTG 3 cut(s) 585, 794, 2559
MflI RGATCY 7 cut(s) 106, 520, 544, 649, 1382, 1978, 2408
MhlI GDGCHC 2 cut(s) 57, 1132
MlyI GAGTC 1 cut(s) 1745
MmeI TCCRAC 5 cut(s) 284, 626, 1203, 1449, 2190
Mph1103I ATGCAT 1 cut(s) 1924
MroXI GAANNNNTTC 1 cut(s) 2298
MseI TTAA 9 cut(s) 48, 318, 566, 753, 777, 830, 1725, 1883, 2387
MslI CAYNNNNRTG 6 cut(s) 663, 1265, 1503, 1629, 1907, 2112
MspA1I CMGCKG 2 cut(s) 76, 377
MspR9I CCNGG 3 cut(s) 786, 1845, 1950
MunI CAATTG 3 cut(s) 585, 794, 2559
MvaI CCWGG 3 cut(s) 786, 1845, 1950
MvnI CGCG 1 cut(s) 997
MwoI GCNNNNNNNGC 7 cut(s) 1031, 1174, 1203, 1812, 1824, 2123, 2330
NdeI CATATG 4 cut(s) 1202, 1390, 1642, 1861
NheI GCTAGC 1 cut(s) 2126
NlaIII CATG 7 cut(s) 1040, 1087, 1138, 1440, 1475, 1827, 2087
NlaIV GGNNCC 3 cut(s) 1411, 1955, 2166
NmuCI GTSAC 5 cut(s) 331, 532, 1565, 1748, 1911
NsbI TGCGCA 1 cut(s) 1362
NsiI ATGCAT 1 cut(s) 1924
NspI RCATGY 2 cut(s) 1827, 2087
NspV TTCGAA 1 cut(s) 43
PaeI GCATGC 1 cut(s) 1827
PciI ACATGT 1 cut(s) 2083
PcsI WCGNNNNNNNCGW 3 cut(s) 257, 511, 1158
PdmI GAANNNNTTC 1 cut(s) 2298
PfeI GAWTC 9 cut(s) 120, 254, 380, 855, 1073, 1116, 1433, 1606, 1973
PflMI CCANNNNNTGG 2 cut(s) 1394, 2039
PkrI GCNGC 6 cut(s) 290, 1027, 1562, 1817, 1840, 2504
PleI GAGTC 1 cut(s) 1745
PpsI GAGTC 1 cut(s) 1745
PpuMI RGGWCCY 1 cut(s) 2164
PscI ACATGT 1 cut(s) 2083
PshBI ATTAAT 1 cut(s) 830
PsiI TTATAA 1 cut(s) 1550
Psp124BI GAGCTC 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 2164
Psp6I CCWGG 3 cut(s) 784, 1843, 1948
PspFI CCCAGC 2 cut(s) 72, 76
PspGI CCWGG 3 cut(s) 784, 1843, 1948
PspN4I GGNNCC 3 cut(s) 1411, 1955, 2166
PspPI GGNCC 2 cut(s) 2164, 2456
PspPPI RGGWCCY 1 cut(s) 2164
PstI CTGCAG 4 cut(s) 293, 559, 1840, 2335
PstNI CAGNNNCTG 1 cut(s) 1844
PsuI RGATCY 7 cut(s) 106, 520, 544, 649, 1382, 1978, 2408
PvuII CAGCTG 2 cut(s) 76, 377
RsaI GTAC 5 cut(s) 241, 760, 1708, 1936, 1969
RsaNI GTAC 5 cut(s) 240, 759, 1707, 1935, 1968
RseI CAYNNNNRTG 6 cut(s) 663, 1265, 1503, 1629, 1907, 2112
SacI GAGCTC 1 cut(s) 57
SaqAI TTAA 9 cut(s) 48, 318, 566, 753, 777, 830, 1725, 1883, 2387
SatI GCNGC 6 cut(s) 289, 1026, 1561, 1816, 1839, 2503
Sau96I GGNCC 2 cut(s) 2164, 2456
SchI GAGTC 1 cut(s) 1745
ScrFI CCNGG 3 cut(s) 786, 1845, 1950
SduI GDGCHC 2 cut(s) 57, 1132
SfaNI GCATC 8 cut(s) 137, 222, 976, 1260, 1266, 1978, 2305, 2514
SfcI CTRYAG 4 cut(s) 289, 555, 1836, 2331
SfuI TTCGAA 1 cut(s) 43
SinI GGWCC 2 cut(s) 2164, 2456
SmiMI CAYNNNNRTG 6 cut(s) 663, 1265, 1503, 1629, 1907, 2112
SmlI CTYRAG 2 cut(s) 56, 1208
SmoI CTYRAG 2 cut(s) 56, 1208
SpeI ACTAGT 1 cut(s) 2258
SphI GCATGC 1 cut(s) 1827
SsiI CCGC 4 cut(s) 479, 997, 1638, 2079
SspI AATATT 1 cut(s) 580
SspMI CTAG 6 cut(s) 306, 548, 1182, 2069, 2127, 2259
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 3 cut(s) 784, 1843, 1948
StyI CCWWGG 1 cut(s) 2281
TaaI ACNGT 7 cut(s) 246, 393, 494, 538, 1244, 1768, 2484
TaiI ACGT 2 cut(s) 342, 2313
TaqI TCGA 6 cut(s) 43, 186, 1096, 1152, 1536, 2417
TatI WGTACW 1 cut(s) 239
TfiI GAWTC 9 cut(s) 120, 254, 380, 855, 1073, 1116, 1433, 1606, 1973
Tru1I TTAA 9 cut(s) 48, 318, 566, 753, 777, 830, 1725, 1883, 2387
Tru9I TTAA 9 cut(s) 48, 318, 566, 753, 777, 830, 1725, 1883, 2387
TscAI CASTG 8 cut(s) 298, 398, 564, 1570, 1624, 1753, 1825, 1909
TseFI GTSAC 5 cut(s) 331, 532, 1565, 1748, 1911
TseI GCWGC 6 cut(s) 288, 1025, 1560, 1815, 1838, 2502
Tsp45I GTSAC 5 cut(s) 331, 532, 1565, 1748, 1911
TspGWI ACGGA 2 cut(s) 266, 2408
TspRI CASTG 8 cut(s) 298, 398, 564, 1570, 1624, 1753, 1825, 1909
Van91I CCANNNNNTGG 2 cut(s) 1394, 2039
VpaK11BI GGWCC 2 cut(s) 2164, 2456
VspI ATTAAT 1 cut(s) 830
XapI RAATTY 5 cut(s) 322, 622, 836, 1275, 1523
XbaI TCTAGA 2 cut(s) 305, 2068
XceI RCATGY 2 cut(s) 1827, 2087
XcmI CCANNNNNNNNNTGG 2 cut(s) 1320, 2119
XmiI GTMKAC 1 cut(s) 691
XmnI GAANNNNTTC 1 cut(s) 2298
XspI CTAG 6 cut(s) 306, 548, 1182, 2069, 2127, 2259
Zsp2I ATGCAT 1 cut(s) 1924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.