RLG00000027534

MIZ/SP-RING zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000955
Physical Location & Seq
Forward (+)
12468649 .. 12480165
11517 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027534

Sequence Viewer

Length: 3519 bp
ATGGATCAAGTGGAGGCACCAATTGTAGATTTCACCCATGTTGGAGCTCTAGCTTTTACTGGCAATGGTGTAGAAGCCGAGTCTTTCAAGCACCGCGTTGACGAAATTTTCGTTCAGGTTGATAAGCTTGAGCTGAGAGTGAATGAGATAGAGAAGTTTTACTCGAGCGCGAGTAAAAAGCAGGGGAATGCAAGTAAGAGTGGCTCAGCTGTGAAGGATAAAGAGAAGCATGTTCCAAGTATGAAGAAGCAGCAGCAAGAGGCTGCATTGAGGGAGGCAGCCGCCGCAAAAAGAATGCAAGAACTTATGCGCCAATTTGGCACTATATTGCGTCAAATTACACAACACAAGTGGGCATGGCCCTTTTTGCAACCTGTAGATGTAGAAGGTCTTGGTTTACATGATTATTATGAGGTCATTGACAAGCCAATGGATTTCAGTACAATTAAAAATCAAATGGAGGCCAAGGATGGTACTGGGTATAAAAACGTAAGGGAGATATGTGCTGATGTGCGGTTAGTTTTCAAAAATGCGATGAGGTATAATGAGGAGAGAAGTGATGTTCATGTTATGGCCAAAACTTTGATGGCGAAATTCGAGGAAAAGTGGCTGCAGCTTCTGCCTAAAGTTACTGAAGAGGAAAAGAGGCTAGAAGAGGAAGAGGCAGAAGCACAGTTAAATATGCAGCTTGCTGAAGAGGCTGCTCATGCTAAAATGGCTAGAGACTTAAGCAATGAGCTTTATGAGGTTGATATGCATTTGGAAGAGCTCCGAGATATGGTTGTTCAAAAGTGCAGAAAAATGTCAACTGAAGAGAAGAGAAAGCTTGGGGTAGCCCTCAGCAGACTGTCCCCAGAGGATCTCAGCACAGCACTAGAGATTGTTGCACAAAGTAATCCAGGTTTTCAAGCTACCGCTGAAGAGGTGGACCTTGATATCGACGCTCAGACTGAATCTACCTTGTGGAGGTTGAAGTTTTTTGTGAAGGACACTTTAGAAGTTCAGGCCAAGAGCTCAGCAAGCATAGGCGCCACCAACAATGACAACACTAACAACCATAACAGCGTAAAACCCACCTCTCAATTCTCCGACTTGTCGTATTGGGTTTTGGGTGGGTTTATTGGTTTTGGAGCTGGAATGGGTTCAGATGCTAAGGAAATGGATTTGGTAACTCAATGCAAGACCAAGTTGGTGTCTTTTCGAGTTAAGGAGCTCAAGGATGTTCTGACCCAGCTGGGTTTTTCAAAGCAAGGGAGGAAGCAGGATCTAATTGACAGAATCTTAGCGTCAGTTTCAGACGAAGAAACATCTAATACACGCAGTTTGTCAAAGAAAAAACACATTGAGAAGAATGGAGTAGTGCAAGTAATTGATGAAGCTTACAGAAAGATGCAGAGTACACCGTCAACGGATTCTGCGACAAAGGAACAAAGTGGTTCAGATAGCTGCAGTGTGAAGCCCTCTAGAAAGGAAGTTAAAAATGTGAATGTGACAGACGTGAAGATTTCTTGCCCCTGTAAAAATCCATTGTCAACTGATTCAACGATACAGTGTGTAGATCCAACATGTCAAGTCCAGCAACATATTGGTTGCGTGATTATTCCTGAGAAAACAAAGGATTGCAGTCCACCAGTTCCGCCTCTTTTCTACTGTGAAATGTGTCGTCTCAAACGAGCAGATCCTGCAAATCCAACTCAGAATGTGGAGAAAAATTTTCAACTTTCCAGAGCCAATAGAGATCTACTTCAGCATAATGAATATGATGTTCAGGCTTGGTGTATACTTCTCAATGACAGTGTTTCATTTAGGTTGCAATGGCCTCAGTTTGCAGAATTACAAGTTAATGGTATGAGTGTTAGGGCAGTTAATAGACCTGGACATCAATTGTTAGGTGCTAATGGCCGTGATGATGGAGCATTAATCACATTATACATTGGGGAGGGAATCAATAAGATTTCATTATCAGGATGTGATAATCGTGCTTTCTGTTTTGGTGTCAGACTGGTAAAACGACGAACATTTCAGCAGGTTCTCAACCTGATTCCTAAAGAAGAAGATGGTGAGCTTTTTGAGGATGCACTTGCGCGGGTTCGTCGTTGCATTGGTGGTGGGGCTGCTATGGCACATGAAGATAGTGATAGTGATTTAGAAGTGATTTCAGATTCAGTTTCTGTTAATCTCCGGTGCCCTATGAGTGGTTCTCGAATGAAGGTAGCTGGCAGATTCAAACCTTGTGCTCACATGGGTTGCTTTGATCTCGAAACTTTCGTGGAGCTAAACCAGCGATCTAGGAAGTGGCAATGCCCCATATGCCTCAAGAACTACTCGTTGGAGGACATCATCGTTGACCGTTATTTCAATCGCATCACAACTATGATGCGAATTTGTGGGGAAGACATAACAGAGATCAATGTGAAGCCAGATGGTTCTTGGAGTGCAAAGACTAAAGGTGAGTTTAGCAACCTTGCGCAATGGCACTTTCCTGATGGATCTCCATATGTTGGTGTGGATTTGGGAACCACAGGCCAGTCCAACCCAGAATCATGTTCAAACCAACACCATAGGCTTATGTCTAATCATGGTGGGGTCAATGAAGTTGGTAAACATCTGGGTGATCCGTTAGACAAAGAATTTGAGGCTTTTGAGCAAAAGGTTATAACAATGAGCAGCAGTGACACTTCCTGTGGCAGAGAGGATGAAGACTTTATTATGAATCAAAAAAGCAGTGTTCATATAGATGTCTCGGCATATGATGACAACGAAGTCAATTCACTTTCTCCTAATTTTGCCTCAACATTTGAAATTGGGAATGGTACTTCTGCTGAAGCTGTTAATGCTGGTATTATTATTCTCAGTGACTCAGAAGATGAAACAGTCAACTTGGTTTCTCCAAGAGTTATCAACAATACTTGCCCAGTAAGCAGCAGTGCATGCTCCCTGTCTGCAGCTCCTGGCATTTCAGACCCATATGCATCTTGTTTGGGTCTTCTAAATGGAAATGGTAATGACACTGGGATGTCACAATATGCATATCCTACTGGTACTCACGCCGATCCTGGGTTCCAATTATTTGGTACTGATTCAGATCTGTCCGATGCTTTTATTGATTTGGAGCAGACATCAGTTGATGGTTCAGCTCCAATGAATGGTAACACACTGGTTTCAGATAAAAATCTAGAACCAAGCGGACATGTCTTAAACTCTTCTGTTTCCCATACCAATGCTAACTTGGCTAGCTTGGTTGATAATCCTTTGGGCTTTGGTAGTGAGGACCCCTCAATGCAAAGTTTTCTTCCAAGTCATCCCTCTTGTTTGTTGGAGCAGTCTGATTTTGGACAACATCAACCAGTCTCAAATGGCATTACTAGTGATTGGGTTGCTCTTAGCCTTGGTATAGGTGAAAGTGTTTCCAATGACGTTGATGTAAACCCTGAACCTGCAGACACAAATGGACTACATTCAAGAAACCAATGTGGATCAACTAAGGGTAAAAATCTTCAATTATTTAATAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04630 ko05160 map04120 map04630 map05160
Pfam Domains
Protein Families
SAP zf-MIZ

Protein Analysis

1173

Amino Acids

129.43

Weight (kDa)

5.12

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 108 - 193 5.6e-17 Bromodomain
BET PF17035 267 - 330 8.3e-18 Bromodomain extra-terminal - transcription regulation
SAP PF02037 397 - 428 7.1e-08 SAP domain
zf-MIZ PF02891 725 - 773 3.8e-19 MIZ/SP-RING zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000955)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970
malus_domestica MD01G1111200.v1.1 MD07G1177400.v1.1
prunus_persica Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1
pyrus_communis pycom01g13860 pycom07g17140
rosa_chinensis RchiOBHm_Chr1g0363931
rosa_laevigata RLG00000027534
rosa_multiflora Rmu_sc0001607.1_g000006
rosa_roxburghii Rroxscaffold_4G00292830
rosa_rugosa Rorug01G0311200 Rorug01G0311300
rosa_samantha Rh1AG319200 Rh1BG282200 Rh1CG298700 Rh1DG313600
rosa_wichuraiana Rw1G028290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2657
AasI GACNNNNNNGTC 1 cut(s) 2762
Acc16I TGCGCA 1 cut(s) 2469
Acc36I ACCTGC 2 cut(s) 2017, 3445
AccB1I GGYRCC 3 cut(s) 16, 1028, 2184
AccB7I CCANNNNNTGG 2 cut(s) 2501, 3146
AccI GTMKAC 1 cut(s) 1780
AccII CGCG 3 cut(s) 96, 170, 2086
AciI CCGC 8 cut(s) 94, 282, 285, 514, 915, 1637, 2086, 3186
AclWI GGATC 9 cut(s) 12, 867, 1272, 1553, 1673, 2497, 2609, 3047, 3484
AcoI YGGCCR 2 cut(s) 573, 1900
AcsI RAATTY 5 cut(s) 105, 593, 1711, 2382, 2630
AcuI CTGAAG 6 cut(s) 654, 714, 831, 939, 1730, 2844
AcyI GRCGYC 1 cut(s) 1029
AfaI GTAC 6 cut(s) 442, 475, 1399, 2815, 3043, 3076
AfiI CCNNNNNNNGG 6 cut(s) 778, 966, 2195, 2501, 3057, 3146
AflII CTTAAG 1 cut(s) 727
AflIII ACRYGT 2 cut(s) 1565, 3190
AhlI ACTAGT 1 cut(s) 3365
AjiI CACGTC 1 cut(s) 1498
AjnI CCWGG 4 cut(s) 898, 1873, 2950, 3055
AjuI GAANNNNNNNTTGG 2 cut(s) 2312, 2344
Alw21I GWGCWC 5 cut(s) 49, 771, 1016, 1215, 2239
Alw26I GTCTC 4 cut(s) 717, 1670, 2746, 3355
AlwI GGATC 9 cut(s) 12, 867, 1272, 1553, 1673, 2497, 2609, 3047, 3484
AlwNI CAGNNNCTG 4 cut(s) 619, 1682, 2171, 2951
Ama87I CYCGRG 1 cut(s) 163
AoxI GGCC 7 cut(s) 359, 462, 573, 1005, 1817, 1900, 2524
ApoI RAATTY 5 cut(s) 105, 593, 1711, 2382, 2630
ArsI GACNNNNNNTTYG 4 cut(s) 92, 124, 2615, 2647
AseI ATTAAT 1 cut(s) 1919
Asp700I GAANNNNTTC 1 cut(s) 1141
AspLEI GCGC 5 cut(s) 170, 312, 1031, 2086, 2470
AspS9I GGNCC 3 cut(s) 360, 928, 3271
AsuHPI GGTGA 5 cut(s) 25, 2072, 2462, 2624, 3410
AsuNHI GCTAGC 1 cut(s) 3233
AvaI CYCGRG 1 cut(s) 163
AvaII GGWCC 2 cut(s) 928, 3271
BaeGI GKGCMC 1 cut(s) 2189
BalI TGGCCA 1 cut(s) 575
BanI GGYRCC 3 cut(s) 16, 1028, 2184
BanII GRGCYC 4 cut(s) 49, 771, 1016, 1215
BbsI GAAGAC 3 cut(s) 2400, 2706, 2978
Bbv12I GWGCWC 5 cut(s) 49, 771, 1016, 1215, 2239
BbvCI CCTCAGC 1 cut(s) 839
BccI CCATC 7 cut(s) 464, 580, 1904, 2051, 2417, 2480, 3122
BceAI ACGGC 1 cut(s) 1887
BciT130I CCWGG 4 cut(s) 900, 1875, 2952, 3057
BcoDI GTCTC 4 cut(s) 717, 1670, 2746, 3355
BcuI ACTAGT 1 cut(s) 3365
BfaI CTAG 9 cut(s) 50, 650, 720, 875, 1464, 2289, 3176, 3234, 3366
BfmI CTRYAG 5 cut(s) 375, 611, 1447, 2943, 3438
BfoI RGCGCY 1 cut(s) 1032
BfrI CTTAAG 1 cut(s) 727
BfuAI ACCTGC 2 cut(s) 2017, 3445
BglI GCCNNNNNGGC 1 cut(s) 318
BglII AGATCT 2 cut(s) 1738, 3085
BlpI GCTNAGC 2 cut(s) 205, 1015
Bme1390I CCNGG 4 cut(s) 900, 1875, 2952, 3057
Bme18I GGWCC 2 cut(s) 928, 3271
BmeT110I CYCGRG 1 cut(s) 163
BmgBI CACGTC 1 cut(s) 1498
BmgT120I GGNCC 3 cut(s) 360, 928, 3271
BmiI GGNNCC 6 cut(s) 18, 1030, 2186, 2518, 3062, 3273
BmrFI CCNGG 4 cut(s) 900, 1875, 2952, 3057
BmrI ACTGGG 3 cut(s) 486, 2909, 3021
BmsI GCATC 7 cut(s) 1138, 1380, 2065, 2367, 2373, 2981, 3085
BmtI GCTAGC 1 cut(s) 3237
BmuI ACTGGG 3 cut(s) 486, 2909, 3021
BpiI GAAGAC 3 cut(s) 2400, 2706, 2978
BplI GAGNNNNNCTC 4 cut(s) 2185, 2217, 3260, 3292
Bpu10I CCTNAGC 2 cut(s) 839, 1152
Bpu1102I GCTNAGC 2 cut(s) 205, 1015
BpuEI CTTGAG 3 cut(s) 149, 1199, 2300
BsaBI GATNNNNATC 2 cut(s) 3084, 3171
BsaHI GRCGYC 1 cut(s) 1029
BsaJI CCNNGG 3 cut(s) 465, 3056, 3388
BsaWI WCCGGW 1 cut(s) 2181
Bsc4I CCNNNNNNNGG 6 cut(s) 778, 966, 2195, 2501, 3057, 3146
Bse3DI GCAATG 5 cut(s) 70, 739, 1820, 2306, 2477
Bse8I GATNNNNATC 2 cut(s) 3084, 3171
BseBI CCWGG 4 cut(s) 900, 1875, 2952, 3057
BseDI CCNNGG 3 cut(s) 465, 3056, 3388
BseGI GGATG 7 cut(s) 475, 1225, 1973, 2080, 2701, 3021, 3301
BseJI GATNNNNATC 2 cut(s) 3084, 3171
BseLI CCNNNNNNNGG 6 cut(s) 778, 966, 2195, 2501, 3057, 3146
BseMI GCAATG 5 cut(s) 70, 739, 1820, 2306, 2477
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 2189
BseYI CCCAGC 2 cut(s) 1230, 1234
BsgI GTGCAG 1 cut(s) 814
Bsh1236I CGCG 3 cut(s) 96, 170, 2086
BshFI GGCC 7 cut(s) 361, 464, 575, 1007, 1819, 1902, 2526
BshNI GGYRCC 3 cut(s) 16, 1028, 2184
BsiHKAI GWGCWC 5 cut(s) 49, 771, 1016, 1215, 2239
BsiHKCI CYCGRG 1 cut(s) 163
BsiSI CCGG 1 cut(s) 2182
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 6 cut(s) 778, 966, 2195, 2501, 3057, 3146
BsmAI GTCTC 4 cut(s) 717, 1670, 2746, 3355
BsmBI CGTCTC 1 cut(s) 1670
BsmFI GGGAC 1 cut(s) 835
BsmI GAATGC 2 cut(s) 193, 300
BsnI GGCC 7 cut(s) 361, 464, 575, 1007, 1819, 1902, 2526
BsoBI CYCGRG 1 cut(s) 163
Bsp1286I GDGCHC 6 cut(s) 49, 771, 1016, 1215, 2189, 2239
Bsp1720I GCTNAGC 2 cut(s) 205, 1015
BspACI CCGC 8 cut(s) 94, 282, 285, 514, 915, 1637, 2086, 3186
BspANI GGCC 7 cut(s) 361, 464, 575, 1007, 1819, 1902, 2526
BspFNI CGCG 3 cut(s) 96, 170, 2086
BspLI GGNNCC 6 cut(s) 18, 1030, 2186, 2518, 3062, 3273
BspMAI CTGCAG 4 cut(s) 615, 1451, 2947, 3442
BspMI ACCTGC 2 cut(s) 2017, 3445
BspOI GCTAGC 1 cut(s) 3237
BspPI GGATC 9 cut(s) 12, 867, 1272, 1553, 1673, 2497, 2609, 3047, 3484
BspQI GCTCTTC 1 cut(s) 759
BspT107I GGYRCC 3 cut(s) 16, 1028, 2184
BspTI CTTAAG 1 cut(s) 727
BsrDI GCAATG 5 cut(s) 70, 739, 1820, 2306, 2477
BssECI CCNNGG 3 cut(s) 465, 3056, 3388
BssNAI GTATAC 1 cut(s) 1781
BssNI GRCGYC 1 cut(s) 1029
BssT1I CCWWGG 2 cut(s) 465, 3388
Bst1107I GTATAC 1 cut(s) 1781
Bst2UI CCWGG 4 cut(s) 900, 1875, 2952, 3057
Bst4CI ACNGT 8 cut(s) 675, 849, 1404, 1551, 1652, 1796, 2351, 2875
Bst6I CTCTTC 9 cut(s) 630, 648, 654, 690, 759, 807, 812, 915, 3208
BstACI GRCGYC 1 cut(s) 1029
BstAFI CTTAAG 1 cut(s) 727
BstAPI GCANNNNNTGC 3 cut(s) 619, 1682, 2931
BstC8I GCNNGC 5 cut(s) 690, 1021, 2218, 2932, 3235
BstENI CCTNNNNNAGG 1 cut(s) 964
BstF5I GGATG 7 cut(s) 475, 1225, 1973, 2080, 2701, 3021, 3301
BstFNI CGCG 3 cut(s) 96, 170, 2086
BstH2I RGCGCY 1 cut(s) 1032
BstHHI GCGC 5 cut(s) 170, 312, 1031, 2086, 2470
BstMAI GTCTC 4 cut(s) 717, 1670, 2746, 3355
BstNI CCWGG 4 cut(s) 900, 1875, 2952, 3057
BstNSI RCATGY 4 cut(s) 233, 1569, 2934, 3194
BstSCI CCNGG 4 cut(s) 898, 1873, 2950, 3055
BstSFI CTRYAG 5 cut(s) 375, 611, 1447, 2943, 3438
BstSLI GKGCMC 1 cut(s) 2189
BstUI CGCG 3 cut(s) 96, 170, 2086
BstV2I GAAGAC 3 cut(s) 2400, 2706, 2978
BstX2I RGATCY 7 cut(s) 859, 1264, 1558, 1678, 1738, 2489, 3085
BstXI CCANNNNNNTGG 1 cut(s) 3071
BstYI RGATCY 7 cut(s) 859, 1264, 1558, 1678, 1738, 2489, 3085
BstZ17I GTATAC 1 cut(s) 1781
BsuRI GGCC 7 cut(s) 361, 464, 575, 1007, 1819, 1902, 2526
BtgZI GCGATG 1 cut(s) 548
BtrI CACGTC 1 cut(s) 1498
BtsCI GGATG 7 cut(s) 475, 1225, 1973, 2080, 2701, 3021, 3301
BtsI GCAGTG 4 cut(s) 1456, 2677, 2731, 2932
BtsIMutI CAGTG 9 cut(s) 1456, 1556, 1801, 2677, 2731, 2860, 2932, 3009, 3155
BveI ACCTGC 2 cut(s) 2017, 3445
Cac8I GCNNGC 5 cut(s) 690, 1021, 2218, 2932, 3235
CaiI CAGNNNCTG 4 cut(s) 619, 1682, 2171, 2951
CfoI GCGC 5 cut(s) 170, 312, 1031, 2086, 2470
Cfr13I GGNCC 3 cut(s) 360, 928, 3271
CseI GACGC 3 cut(s) 320, 950, 1275
Csp6I GTAC 6 cut(s) 441, 474, 1398, 2814, 3042, 3075
CspCI CAANNNNNGTGG 2 cut(s) 332, 367
CviQI GTAC 6 cut(s) 441, 474, 1398, 2814, 3042, 3075
DinI GGCGCC 1 cut(s) 1030
DrdI GACNNNNNNGTC 1 cut(s) 2762
DseDI GACNNNNNNGTC 1 cut(s) 2762
EaeI YGGCCR 2 cut(s) 573, 1900
Eam1104I CTCTTC 9 cut(s) 630, 648, 654, 690, 759, 807, 812, 915, 3208
EarI CTCTTC 9 cut(s) 630, 648, 654, 690, 759, 807, 812, 915, 3208
EciI GGCGGA 1 cut(s) 1626
Ecl136II GAGCTC 4 cut(s) 47, 769, 1014, 1213
Eco130I CCWWGG 2 cut(s) 465, 3388
Eco24I GRGCYC 4 cut(s) 49, 771, 1016, 1215
Eco32I GATATC 1 cut(s) 937
Eco47I GGWCC 2 cut(s) 928, 3271
Eco53kI GAGCTC 4 cut(s) 47, 769, 1014, 1213
Eco57I CTGAAG 6 cut(s) 654, 714, 831, 939, 1730, 2844
Eco88I CYCGRG 1 cut(s) 163
EcoICRI GAGCTC 4 cut(s) 47, 769, 1014, 1213
EcoNI CCTNNNNNAGG 1 cut(s) 964
EcoO109I RGGNCCY 1 cut(s) 3271
EcoRII CCWGG 4 cut(s) 898, 1873, 2950, 3055
EcoRV GATATC 1 cut(s) 937
EcoT14I CCWWGG 2 cut(s) 465, 3388
EcoT22I ATGCAT 3 cut(s) 759, 2974, 3031
EcoT38I GRGCYC 4 cut(s) 49, 771, 1016, 1215
EgeI GGCGCC 1 cut(s) 1030
EheI GGCGCC 1 cut(s) 1030
ErhI CCWWGG 2 cut(s) 465, 3388
Esp3I CGTCTC 1 cut(s) 1670
FaqI GGGAC 1 cut(s) 835
FauI CCCGC 1 cut(s) 2079
FauNDI CATATG 4 cut(s) 2309, 2497, 2749, 2968
FblI GTMKAC 1 cut(s) 1780
FokI GGATG 7 cut(s) 482, 1232, 1980, 2087, 2708, 3028, 3288
FriOI GRGCYC 4 cut(s) 49, 771, 1016, 1215
FspBI CTAG 9 cut(s) 50, 650, 720, 875, 1464, 2289, 3176, 3234, 3366
FspI TGCGCA 1 cut(s) 2469
GlaI GCGC 5 cut(s) 169, 311, 1030, 2085, 2469
GsaI CCCAGC 2 cut(s) 1234, 1238
HaeII RGCGCY 1 cut(s) 1032
HaeIII GGCC 7 cut(s) 361, 464, 575, 1007, 1819, 1902, 2526
HapII CCGG 1 cut(s) 2182
HgaI GACGC 3 cut(s) 320, 950, 1275
HhaI GCGC 5 cut(s) 170, 312, 1031, 2086, 2470
Hin1I GRCGYC 1 cut(s) 1029
Hin6I GCGC 5 cut(s) 168, 310, 1029, 2084, 2468
HinP1I GCGC 5 cut(s) 168, 310, 1029, 2084, 2468
HincII GTYRAC 6 cut(s) 100, 807, 1407, 1533, 2347, 2878
HindII GTYRAC 6 cut(s) 100, 807, 1407, 1533, 2347, 2878
HindIII AAGCTT 3 cut(s) 125, 824, 1377
HpaII CCGG 1 cut(s) 2182
HphI GGTGA 5 cut(s) 25, 2072, 2462, 2624, 3410
Hpy99I CGWCG 3 cut(s) 944, 2016, 2097
HpyAV CCTTC 4 cut(s) 208, 380, 979, 2203
HpyCH4III ACNGT 8 cut(s) 675, 849, 1404, 1551, 1652, 1796, 2351, 2875
HpyCH4IV ACGT 3 cut(s) 489, 1497, 3417
HpySE526I ACGT 3 cut(s) 489, 1497, 3417
Hsp92I GRCGYC 1 cut(s) 1029
HspAI GCGC 5 cut(s) 168, 310, 1029, 2084, 2468
KasI GGCGCC 1 cut(s) 1028
LguI GCTCTTC 1 cut(s) 759
LweI GCATC 7 cut(s) 1138, 1380, 2065, 2367, 2373, 2981, 3085
MaeI CTAG 9 cut(s) 50, 650, 720, 875, 1464, 2289, 3176, 3234, 3366
MaeII ACGT 3 cut(s) 489, 1497, 3417
MaeIII GTNAC 7 cut(s) 628, 1168, 1489, 2672, 2855, 3018, 3149
MfeI CAATTG 2 cut(s) 21, 1883
MflI RGATCY 7 cut(s) 859, 1264, 1558, 1678, 1738, 2489, 3085
MhlI GDGCHC 6 cut(s) 49, 771, 1016, 1215, 2189, 2239
MlsI TGGCCA 1 cut(s) 575
MluNI TGGCCA 1 cut(s) 575
Mly113I GGCGCC 1 cut(s) 1029
MlyI GAGTC 2 cut(s) 89, 2852
MmeI TCCRAC 7 cut(s) 22, 1113, 1586, 1715, 2310, 2556, 3297
Mox20I TGGCCA 1 cut(s) 575
Mph1103I ATGCAT 3 cut(s) 759, 2974, 3031
MroXI GAANNNNTTC 1 cut(s) 1141
MscI TGGCCA 1 cut(s) 575
MslI CAYNNNNRTG 5 cut(s) 2372, 2610, 2736, 3014, 3219
Msp20I TGGCCA 1 cut(s) 575
MspA1I CMGCKG 3 cut(s) 209, 917, 1234
MspCI CTTAAG 1 cut(s) 727
MspI CCGG 1 cut(s) 2182
MspR9I CCNGG 4 cut(s) 900, 1875, 2952, 3057
MunI CAATTG 2 cut(s) 21, 1883
Mva1269I GAATGC 2 cut(s) 193, 300
MvaI CCWGG 4 cut(s) 900, 1875, 2952, 3057
MvnI CGCG 3 cut(s) 96, 170, 2086
NarI GGCGCC 1 cut(s) 1029
NdeI CATATG 4 cut(s) 2309, 2497, 2749, 2968
NheI GCTAGC 1 cut(s) 3233
NlaIV GGNNCC 6 cut(s) 18, 1030, 2186, 2518, 3062, 3273
NmeAIII GCCGAG 2 cut(s) 103, 2723
NmuCI GTSAC 4 cut(s) 1489, 2672, 2855, 3018
NsbI TGCGCA 1 cut(s) 2469
NsiI ATGCAT 3 cut(s) 759, 2974, 3031
NspI RCATGY 4 cut(s) 233, 1569, 2934, 3194
PaeI GCATGC 1 cut(s) 2934
PaeR7I CTCGAG 1 cut(s) 163
PciI ACATGT 2 cut(s) 1565, 3190
PciSI GCTCTTC 1 cut(s) 759
PcsI WCGNNNNNNNCGW 4 cut(s) 108, 1415, 1669, 2265
PctI GAATGC 2 cut(s) 193, 300
PdmI GAANNNNTTC 1 cut(s) 1141
PflMI CCANNNNNTGG 2 cut(s) 2501, 3146
PleI GAGTC 2 cut(s) 88, 2852
PluTI GGCGCC 1 cut(s) 1032
PpsI GAGTC 2 cut(s) 88, 2852
PpuMI RGGWCCY 1 cut(s) 3271
PscI ACATGT 2 cut(s) 1565, 3190
PshBI ATTAAT 1 cut(s) 1919
PsiI TTATAA 1 cut(s) 2657
Psp124BI GAGCTC 4 cut(s) 49, 771, 1016, 1215
Psp5II RGGWCCY 1 cut(s) 3271
Psp6I CCWGG 4 cut(s) 898, 1873, 2950, 3055
PspFI CCCAGC 2 cut(s) 1230, 1234
PspGI CCWGG 4 cut(s) 898, 1873, 2950, 3055
PspN4I GGNNCC 6 cut(s) 18, 1030, 2186, 2518, 3062, 3273
PspPI GGNCC 3 cut(s) 360, 928, 3271
PspPPI RGGWCCY 1 cut(s) 3271
PspXI VCTCGAGB 1 cut(s) 163
PstI CTGCAG 4 cut(s) 615, 1451, 2947, 3442
PstNI CAGNNNCTG 4 cut(s) 619, 1682, 2171, 2951
PsuI RGATCY 7 cut(s) 859, 1264, 1558, 1678, 1738, 2489, 3085
PvuII CAGCTG 2 cut(s) 209, 1234
RsaI GTAC 6 cut(s) 442, 475, 1399, 2815, 3043, 3076
RsaNI GTAC 6 cut(s) 441, 474, 1398, 2814, 3042, 3075
RseI CAYNNNNRTG 5 cut(s) 2372, 2610, 2736, 3014, 3219
SacI GAGCTC 4 cut(s) 49, 771, 1016, 1215
SapI GCTCTTC 1 cut(s) 759
Sau96I GGNCC 3 cut(s) 360, 928, 3271
SchI GAGTC 2 cut(s) 89, 2852
ScrFI CCNGG 4 cut(s) 900, 1875, 2952, 3057
SduI GDGCHC 6 cut(s) 49, 771, 1016, 1215, 2189, 2239
SfaNI GCATC 7 cut(s) 1138, 1380, 2065, 2367, 2373, 2981, 3085
SfcI CTRYAG 5 cut(s) 375, 611, 1447, 2943, 3438
SfoI GGCGCC 1 cut(s) 1030
Sfr274I CTCGAG 1 cut(s) 163
SinI GGWCC 2 cut(s) 928, 3271
SlaI CTCGAG 1 cut(s) 163
SmiMI CAYNNNNRTG 5 cut(s) 2372, 2610, 2736, 3014, 3219
SmlI CTYRAG 5 cut(s) 128, 163, 727, 1214, 2315
SmoI CTYRAG 5 cut(s) 128, 163, 727, 1214, 2315
SpeI ACTAGT 1 cut(s) 3365
SphI GCATGC 1 cut(s) 2934
SsiI CCGC 8 cut(s) 94, 282, 285, 514, 915, 1637, 2086, 3186
SspDI GGCGCC 1 cut(s) 1028
SspMI CTAG 9 cut(s) 50, 650, 720, 875, 1464, 2289, 3176, 3234, 3366
SstI GAGCTC 4 cut(s) 49, 771, 1016, 1215
StyD4I CCNGG 4 cut(s) 898, 1873, 2950, 3055
StyI CCWWGG 2 cut(s) 465, 3388
TaaI ACNGT 8 cut(s) 675, 849, 1404, 1551, 1652, 1796, 2351, 2875
TaiI ACGT 3 cut(s) 492, 1500, 3420
TaqI TCGA 6 cut(s) 164, 597, 939, 1201, 2203, 2259
TatI WGTACW 2 cut(s) 440, 1397
TauI GCSGC 2 cut(s) 284, 287
TscAI CASTG 9 cut(s) 1456, 1556, 1801, 2677, 2731, 2860, 2932, 3016, 3162
TseFI GTSAC 4 cut(s) 1489, 2672, 2855, 3018
Tsp45I GTSAC 4 cut(s) 1489, 2672, 2855, 3018
TspGWI ACGGA 2 cut(s) 1424, 2607
TspRI CASTG 9 cut(s) 1456, 1556, 1801, 2677, 2731, 2860, 2932, 3016, 3162
Van91I CCANNNNNTGG 2 cut(s) 2501, 3146
Vha464I CTTAAG 1 cut(s) 727
VpaK11BI GGWCC 2 cut(s) 928, 3271
VspI ATTAAT 1 cut(s) 1919
XagI CCTNNNNNAGG 1 cut(s) 964
XapI RAATTY 5 cut(s) 105, 593, 1711, 2382, 2630
XbaI TCTAGA 2 cut(s) 1463, 3175
XceI RCATGY 4 cut(s) 233, 1569, 2934, 3194
XcmI CCANNNNNNNNNTGG 4 cut(s) 583, 1583, 2427, 3226
XhoI CTCGAG 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 1780
XmnI GAANNNNTTC 1 cut(s) 1141
XspI CTAG 9 cut(s) 50, 650, 720, 875, 1464, 2289, 3176, 3234, 3366
Zsp2I ATGCAT 3 cut(s) 759, 2974, 3031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.