Rh1BG282200

MIZ/SP-RING zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000955
Physical Location & Seq
Reverse (-)
41884358 .. 41894062
9705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG282200.1

Sequence Viewer

Length: 2565 bp
ATGGGTTCAGATGCTAAGGAAATGGATTTGGTAACTCAATGCAAGACCAAGTTGGTGTCTTTTCGAGTTAAGGAGCTCAAGGATGTTCTGACCCAGCTGGGTTTTTCAAAGCAAGGGAGGAAGCAGGATCTAATTGACAGAATCTTAGCATCAGTTTCGGATGAAGAAACATCCAATACACGCAGTTTGTCAAAGAAAAAACACATTGAGAAGAATGGAGTAGTGCAAGTAATTGATGAAGCTTACAGAAAGATGCAGAGTACACCGTCAACGGATTCTCCGACAAAGGAACAAAGTGGTTCAGATGGCTGCAGTGTGAAGCTCTCTAGAAAGGAAGTTAAAAATTTGAATGTGACAGACGTGAAGATTTCTTGCCCCTGTAAAAGTTCCTTGTCAACTGATTCAATGATACAGTGTGTAGATCCAATATGTCAAGTTCAGCAACATATTGGTTGCGTGATTATTCCTGAGAAAACAAAGGATTGCAGTCCACCAGTTCCACCTCTTTTCTACTGTGAAATGTGTCGTCTCAAACGAGCAGATCCTTACTGGGTGACTGTGGTGGATCTAGTATCTGCAGTGAAGTTAAGTGCTTCAAATATTTCAATTGATGGTGCAAATCCTACTCAGAATGTGGAGAAAAATTTTCAACTTTCCAGAGCCAATAGAGATCTACTTCAGCATAATGAATATGATGTTCAGGCTTGGTGTATACTTCTCAATGATAGTGTTTCATTTAGGTTGCAATGGCCTCAGTTTGCAGATTTACAAGTTAATGGTACGAGTGTTAGGGCAGTTAATAGACCTGGACATCAATTGTTAGGTGCTAATGGCCGTGATGATGGAGCATTAATCACATTTTGCATTGGGGAGGGAATCAATAAGATTTCATTATCAGGATGTGATAATCGTGCTTTCTGTTTTGGCGTCAGACTGGTAAAACGACGAACATTTCAGCAGGTTCTCAACCTGATTCCTAAAGAAGAAGATGGTGAGCTTTTTGAGGATGCACTTGCGCGGGTTCGTCGTTGCATTGGTGGTGGGGCTGCTATGGCACATGAAGATAGTGATAGTGATTTAGAAGTGATTTCAGATTCAGTTTCCGTTAATCTCCGGTGCCCTATGAGTGGTTCTCGAATGAAGGTAGCTGGCAGATTCAAACCTTGTGCTCACATGGGTTGCTTTGATCTCGAAACTTTCGTGGAGCTAAACCAGCGATCTAGGAAGTGGCAATGCCCCATATGCCTCAAGAACTACTCGTTGGAGGACATCATCATTGACCGTTATTTCAATCGCATCACAACTATGATGCGAATTTGTGGGGAAGACATAACAGAGATCAATGTGAAGCCAGATGGTTCTTGGAGTGCAAAGACTAAAGGTGAGTTTAGCGACCTTGCGCAATGGCACTTTCCTGATGGATCTCCATATGTTGGTATGGATTTGGGAACCACAGGCCAGTCCAACCCAGAATCATGTTCAAACCAACACCATAGGTTTATGTCTAATCATGGTGGGGTCAGTGAAGTTGGTAAACATCCGGGTGATCCATTAGACAAAGAATTTGAGGCTTTCGAGCAAAAGGTTATAACAATGAGCAGCAGTGACACTTCCTGTGGCAGAGAGGATGAAGACTTTATTATGAATCAAAAAAGCAGTGTTCATATAGATGTCTCCGCTTATGATGACAACGAAGTCAATTCACTTTCTCCTAATTTTGCCTCAACATTTGAAATTGGGAATGGTGCTGAAGCTGTTAATGCTGGTATTATTATTCTCAGTGACTCAGAAGATGAAACAGTCAACTTGGTTTCTCCAAGAGTTATCAACAATACTTGCCCAGTAAGCAGCAGTGCATGCTCCCTGTCTGCAGCTCCTGGCATTTCAGACCCATATGCGTCTTGTTTGGGTCTTTTAAATGGAAATGGTAATGACACTGGGATGTCACAATATGCATATCCTACTGGTACTCACGCTGATCCTGGGTTCCAATTGTTTGGTACTGATTCAGATCTCTCCGATGCTTTTATTGATTTGGAGCAGACATCAGTTGACGGTTCAGCTCCAATGAATGGTAACACATTGGTTTCAGATAAAAATCTAGAACCAAGTGGGCATGTCTTAAACTCTTCTGTTTCCCATACCAATGCTAACTTGGCTAGCTTGGTTGATAATCCTTTGGGCTTTGGTAGTGAGGACCCCTCAATGCAAAGTTTTCTTCCAAGTCATCCCTCTTGTTTGTTGGAGCAGTCTGATTTTGGACAACATCAACCAGTCTCAAATGGCATTACTAGTGATTGGGTTGCTCTTAGCCTTGGTATAGGTGAAAGTGTTCCCAATGACGTTGATGCAAATGCTGAACCTGCAGACACAAATGGACTATATTCAAGAAACCAATGTGGATCAAATAAGGTGGCTTTAATCACGGATAGAAATGAGGGATCTAAGTCGAAATCTAACAGAGCAAATATAAGGAAACTTTCAGATGGACCTTTCTCCTTTCCCCGTCAGCCACGGTCTGTGAGACCGCGAGTGTATCTGTCAGTAGATTCCAATTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04630 ko05160 map04120 map04630 map05160
Pfam Domains
Protein Families
SAP zf-MIZ

Protein Analysis

854

Amino Acids

93.21

Weight (kDa)

5.1

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP PF02037 18 - 49 4.9e-08 SAP domain
zf-MIZ PF02891 369 - 417 2.6e-19 MIZ/SP-RING zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000955)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970 FvH4_7g19970
malus_domestica MD01G1111200.v1.1 MD07G1177400.v1.1
prunus_persica Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1 Prupe.2G216700_v2.0.a1
pyrus_communis pycom01g13860 pycom07g17140
rosa_chinensis RchiOBHm_Chr1g0363931
rosa_laevigata RLG00000027534
rosa_multiflora Rmu_sc0001607.1_g000006
rosa_roxburghii Rroxscaffold_4G00292830
rosa_rugosa Rorug01G0311200 Rorug01G0311300
rosa_samantha Rh1AG319200 Rh1BG282200 Rh1CG298700 Rh1DG313600
rosa_wichuraiana Rw1G028290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1589
AasI GACNNNNNNGTC 1 cut(s) 1694
Acc16I TGCGCA 1 cut(s) 1401
Acc36I ACCTGC 2 cut(s) 949, 2371
AccB1I GGYRCC 1 cut(s) 1116
AccB7I CCANNNNNTGG 2 cut(s) 1433, 2072
AccI GTMKAC 1 cut(s) 712
AccII CGCG 2 cut(s) 1018, 2530
AciI CCGC 3 cut(s) 1018, 1677, 2528
AclWI GGATC 9 cut(s) 135, 416, 536, 573, 1429, 1541, 1973, 2410, 2449
AcoI YGGCCR 1 cut(s) 832
AcsI RAATTY 4 cut(s) 343, 643, 1314, 1562
AcuI CTGAAG 2 cut(s) 662, 1770
AcyI GRCGYC 1 cut(s) 927
AfaI GTAC 4 cut(s) 262, 781, 1969, 2002
AfiI CCNNNNNNNGG 3 cut(s) 1127, 1433, 2072
AhlI ACTAGT 1 cut(s) 2291
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 3 cut(s) 805, 1876, 1981
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
Alw21I GWGCWC 2 cut(s) 78, 1171
Alw26I GTCTC 4 cut(s) 533, 1678, 2281, 2518
AlwI GGATC 9 cut(s) 135, 416, 536, 573, 1429, 1541, 1973, 2410, 2449
AlwNI CAGNNNCTG 1 cut(s) 1877
AoxI GGCC 3 cut(s) 749, 832, 1456
ApeKI GCWGC 5 cut(s) 309, 1046, 1599, 1848, 1871
ApoI RAATTY 4 cut(s) 343, 643, 1314, 1562
ArsI GACNNNNNNTTYG 2 cut(s) 1547, 1579
AseI ATTAAT 1 cut(s) 851
Asp700I GAANNNNTTC 1 cut(s) 2331
AspLEI GCGC 2 cut(s) 1018, 1402
AspS9I GGNCC 2 cut(s) 2197, 2489
AsuC2I CCSGG 1 cut(s) 1542
AsuHPI GGTGA 5 cut(s) 565, 1004, 1394, 1556, 2336
AsuNHI GCTAGC 1 cut(s) 2159
AvaII GGWCC 2 cut(s) 2197, 2489
BaeGI GKGCMC 1 cut(s) 1121
BaeI ACNNNNGTAYC 2 cut(s) 771, 804
BanI GGYRCC 1 cut(s) 1116
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 2 cut(s) 1332, 1638
Bbv12I GWGCWC 2 cut(s) 78, 1171
BbvI GCAGC 5 cut(s) 296, 1033, 1611, 1860, 1883
BccI CCATC 7 cut(s) 299, 605, 836, 983, 1349, 1412, 2480
BceAI ACGGC 1 cut(s) 819
BcgI CGANNNNNNTGC 2 cut(s) 138, 172
BciT130I CCWGG 3 cut(s) 807, 1878, 1983
BcnI CCSGG 1 cut(s) 1542
BcoDI GTCTC 4 cut(s) 533, 1678, 2281, 2518
BcuI ACTAGT 1 cut(s) 2291
BfaI CTAG 6 cut(s) 327, 569, 1221, 2102, 2160, 2292
BfmI CTRYAG 4 cut(s) 310, 576, 1869, 2364
BfuAI ACCTGC 2 cut(s) 949, 2371
BglII AGATCT 2 cut(s) 670, 2011
BisI GCNGC 5 cut(s) 310, 1047, 1600, 1849, 1872
BlsI GCNGC 5 cut(s) 311, 1048, 1601, 1850, 1873
Bme1390I CCNGG 4 cut(s) 807, 1542, 1878, 1983
Bme18I GGWCC 2 cut(s) 2197, 2489
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 2 cut(s) 2197, 2489
BmiI GGNNCC 4 cut(s) 1118, 1450, 1988, 2199
BmrFI CCNGG 4 cut(s) 807, 1542, 1878, 1983
BmrI ACTGGG 3 cut(s) 559, 1835, 1947
BmsI GCATC 7 cut(s) 158, 243, 997, 1299, 1305, 2011, 2338
BmtI GCTAGC 1 cut(s) 2163
BmuI ACTGGG 3 cut(s) 559, 1835, 1947
BpiI GAAGAC 2 cut(s) 1332, 1638
BplI GAGNNNNNCTC 4 cut(s) 1117, 1149, 2186, 2218
Bpu10I CCTNAGC 1 cut(s) 15
BpuEI CTTGAG 2 cut(s) 62, 1232
BpuMI CCSGG 1 cut(s) 1542
BsaBI GATNNNNATC 2 cut(s) 2010, 2097
BsaHI GRCGYC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 2518
BsaJI CCNNGG 3 cut(s) 1982, 2314, 2513
BsaWI WCCGGW 1 cut(s) 1113
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bsc4I CCNNNNNNNGG 3 cut(s) 1127, 1433, 2072
Bse1I ACTGG 8 cut(s) 494, 554, 939, 1459, 1841, 1942, 1969, 2273
Bse3DI GCAATG 3 cut(s) 752, 1238, 1409
Bse8I GATNNNNATC 2 cut(s) 2010, 2097
BseBI CCWGG 3 cut(s) 807, 1878, 1983
BseDI CCNNGG 3 cut(s) 1982, 2314, 2513
BseGI GGATG 9 cut(s) 88, 166, 170, 905, 1012, 1537, 1633, 1947, 2227
BseJI GATNNNNATC 2 cut(s) 2010, 2097
BseLI CCNNNNNNNGG 3 cut(s) 1127, 1433, 2072
BseMI GCAATG 3 cut(s) 752, 1238, 1409
BseMII CTCAG 5 cut(s) 459, 641, 767, 1792, 1800
BseNI ACTGG 8 cut(s) 494, 554, 939, 1459, 1841, 1942, 1969, 2273
BseSI GKGCMC 1 cut(s) 1121
BseXI GCAGC 5 cut(s) 296, 1033, 1611, 1860, 1883
BseYI CCCAGC 2 cut(s) 93, 97
Bsh1236I CGCG 2 cut(s) 1018, 2530
BshFI GGCC 3 cut(s) 751, 834, 1458
BshNI GGYRCC 1 cut(s) 1116
BsiHKAI GWGCWC 2 cut(s) 78, 1171
BsiSI CCGG 2 cut(s) 1114, 1541
BslI CCNNNNNNNGG 3 cut(s) 1127, 1433, 2072
BsmAI GTCTC 4 cut(s) 533, 1678, 2281, 2518
BsmBI CGTCTC 1 cut(s) 533
BsnI GGCC 3 cut(s) 751, 834, 1458
Bso31I GGTCTC 1 cut(s) 2518
Bsp1286I GDGCHC 3 cut(s) 78, 1121, 1171
BspACI CCGC 3 cut(s) 1018, 1677, 2528
BspANI GGCC 3 cut(s) 751, 834, 1458
BspCNI CTCAG 5 cut(s) 460, 640, 766, 1791, 1799
BspFNI CGCG 2 cut(s) 1018, 2530
BspLI GGNNCC 4 cut(s) 1118, 1450, 1988, 2199
BspMAI CTGCAG 4 cut(s) 314, 580, 1873, 2368
BspMI ACCTGC 2 cut(s) 949, 2371
BspOI GCTAGC 1 cut(s) 2163
BspPI GGATC 9 cut(s) 135, 416, 536, 573, 1429, 1541, 1973, 2410, 2449
BspT107I GGYRCC 1 cut(s) 1116
BspTNI GGTCTC 1 cut(s) 2518
BsrDI GCAATG 3 cut(s) 752, 1238, 1409
BsrI ACTGG 8 cut(s) 494, 554, 939, 1459, 1841, 1942, 1969, 2273
BssECI CCNNGG 3 cut(s) 1982, 2314, 2513
BssNAI GTATAC 1 cut(s) 713
BssNI GRCGYC 1 cut(s) 927
BssT1I CCWWGG 1 cut(s) 2314
Bst1107I GTATAC 1 cut(s) 713
Bst2UI CCWGG 3 cut(s) 807, 1878, 1983
Bst4CI ACNGT 8 cut(s) 267, 414, 515, 559, 1283, 1801, 2057, 2517
Bst6I CTCTTC 1 cut(s) 2134
BstACI GRCGYC 1 cut(s) 927
BstAPI GCANNNNNTGC 1 cut(s) 1857
BstC8I GCNNGC 3 cut(s) 1150, 1858, 2161
BstDEI CTNAG 9 cut(s) 15, 145, 468, 627, 753, 1778, 1786, 2309, 2445
BstDSI CCRYGG 1 cut(s) 2513
BstF5I GGATG 9 cut(s) 88, 166, 170, 905, 1012, 1537, 1633, 1947, 2227
BstFNI CGCG 2 cut(s) 1018, 2530
BstHHI GCGC 2 cut(s) 1018, 1402
BstMAI GTCTC 4 cut(s) 533, 1678, 2281, 2518
BstMWI GCNNNNNNNGC 8 cut(s) 1052, 1213, 1242, 1760, 1845, 1857, 2156, 2363
BstNI CCWGG 3 cut(s) 807, 1878, 1983
BstNSI RCATGY 2 cut(s) 1860, 2120
BstSCI CCNGG 4 cut(s) 805, 1540, 1876, 1981
BstSFI CTRYAG 4 cut(s) 310, 576, 1869, 2364
BstSLI GKGCMC 1 cut(s) 1121
BstUI CGCG 2 cut(s) 1018, 2530
BstV1I GCAGC 5 cut(s) 296, 1033, 1611, 1860, 1883
BstV2I GAAGAC 2 cut(s) 1332, 1638
BstX2I RGATCY 8 cut(s) 127, 421, 541, 565, 670, 1421, 2011, 2441
BstXI CCANNNNNNTGG 1 cut(s) 1997
BstYI RGATCY 8 cut(s) 127, 421, 541, 565, 670, 1421, 2011, 2441
BstZ17I GTATAC 1 cut(s) 713
BsuRI GGCC 3 cut(s) 751, 834, 1458
BtgI CCRYGG 1 cut(s) 2513
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 9 cut(s) 88, 166, 170, 905, 1012, 1537, 1633, 1947, 2227
BtsI GCAGTG 5 cut(s) 319, 585, 1609, 1663, 1858
BtsIMutI CAGTG 9 cut(s) 319, 419, 585, 1528, 1609, 1663, 1786, 1858, 1935
BveI ACCTGC 2 cut(s) 949, 2371
Cac8I GCNNGC 3 cut(s) 1150, 1858, 2161
CaiI CAGNNNCTG 1 cut(s) 1877
CfoI GCGC 2 cut(s) 1018, 1402
Cfr13I GGNCC 2 cut(s) 2197, 2489
CseI GACGC 2 cut(s) 916, 1887
Csp6I GTAC 4 cut(s) 261, 780, 1968, 2001
CspCI CAANNNNNGTGG 2 cut(s) 2394, 2429
CviAII CATG 6 cut(s) 1058, 1174, 1476, 1511, 1857, 2117
CviQI GTAC 4 cut(s) 261, 780, 1968, 2001
DdeI CTNAG 9 cut(s) 15, 145, 468, 627, 753, 1778, 1786, 2309, 2445
DraI TTTAAA 1 cut(s) 1917
DrdI GACNNNNNNGTC 1 cut(s) 1694
DseDI GACNNNNNNGTC 1 cut(s) 1694
EaeI YGGCCR 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 2134
EarI CTCTTC 1 cut(s) 2134
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 2314
Eco24I GRGCYC 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 2518
Eco47I GGWCC 2 cut(s) 2197, 2489
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 2 cut(s) 662, 1770
EcoICRI GAGCTC 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 2197
EcoRII CCWGG 3 cut(s) 805, 1876, 1981
EcoT14I CCWWGG 1 cut(s) 2314
EcoT22I ATGCAT 1 cut(s) 1957
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 2314
Esp3I CGTCTC 1 cut(s) 533
FaeI CATG 6 cut(s) 1061, 1177, 1479, 1514, 1860, 2120
FatI CATG 6 cut(s) 1057, 1173, 1475, 1510, 1856, 2116
FauI CCCGC 1 cut(s) 1011
FauNDI CATATG 3 cut(s) 1241, 1429, 1894
FblI GTMKAC 1 cut(s) 712
Fnu4HI GCNGC 5 cut(s) 310, 1047, 1600, 1849, 1872
FokI GGATG 9 cut(s) 95, 157, 173, 912, 1019, 1524, 1640, 1954, 2214
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 5 cut(s) 310, 1047, 1600, 1849, 1872
FspBI CTAG 6 cut(s) 327, 569, 1221, 2102, 2160, 2292
FspI TGCGCA 1 cut(s) 1401
GlaI GCGC 2 cut(s) 1017, 1401
GluI GCNGC 5 cut(s) 310, 1047, 1600, 1849, 1872
GsaI CCCAGC 2 cut(s) 97, 101
HaeIII GGCC 3 cut(s) 751, 834, 1458
HapII CCGG 2 cut(s) 1114, 1541
HgaI GACGC 2 cut(s) 916, 1887
HhaI GCGC 2 cut(s) 1018, 1402
Hin1I GRCGYC 1 cut(s) 927
Hin1II CATG 6 cut(s) 1061, 1177, 1479, 1514, 1860, 2120
Hin6I GCGC 2 cut(s) 1016, 1400
HinP1I GCGC 2 cut(s) 1016, 1400
HincII GTYRAC 4 cut(s) 270, 396, 1804, 2053
HindII GTYRAC 4 cut(s) 270, 396, 1804, 2053
HindIII AAGCTT 1 cut(s) 240
HpaII CCGG 2 cut(s) 1114, 1541
HphI GGTGA 5 cut(s) 565, 1004, 1394, 1556, 2336
Hpy166II GTNNAC 8 cut(s) 263, 270, 396, 491, 713, 1535, 1804, 2053
Hpy8I GTNNAC 8 cut(s) 263, 270, 396, 491, 713, 1535, 1804, 2053
Hpy99I CGWCG 2 cut(s) 948, 1029
HpyAV CCTTC 1 cut(s) 1135
HpyCH4III ACNGT 8 cut(s) 267, 414, 515, 559, 1283, 1801, 2057, 2517
HpyCH4IV ACGT 2 cut(s) 360, 2343
HpyF10VI GCNNNNNNNGC 8 cut(s) 1052, 1213, 1242, 1760, 1845, 1857, 2156, 2363
HpyF3I CTNAG 9 cut(s) 15, 145, 468, 627, 753, 1778, 1786, 2309, 2445
HpySE526I ACGT 2 cut(s) 360, 2343
Hsp92I GRCGYC 1 cut(s) 927
Hsp92II CATG 6 cut(s) 1061, 1177, 1479, 1514, 1860, 2120
HspAI GCGC 2 cut(s) 1016, 1400
LmnI GCTCC 8 cut(s) 73, 845, 1204, 1865, 1879, 2038, 2068, 2245
Lsp1109I GCAGC 5 cut(s) 296, 1033, 1611, 1860, 1883
LweI GCATC 7 cut(s) 158, 243, 997, 1299, 1305, 2011, 2338
MaeI CTAG 6 cut(s) 327, 569, 1221, 2102, 2160, 2292
MaeII ACGT 2 cut(s) 360, 2343
MaeIII GTNAC 7 cut(s) 31, 352, 553, 1604, 1781, 1944, 2075
MfeI CAATTG 3 cut(s) 606, 815, 1991
MflI RGATCY 8 cut(s) 127, 421, 541, 565, 670, 1421, 2011, 2441
MhlI GDGCHC 3 cut(s) 78, 1121, 1171
MlyI GAGTC 1 cut(s) 1778
MmeI TCCRAC 4 cut(s) 305, 1242, 1488, 2223
Mph1103I ATGCAT 1 cut(s) 1957
MroXI GAANNNNTTC 1 cut(s) 2331
MslI CAYNNNNRTG 5 cut(s) 1304, 1542, 1668, 1940, 2145
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 2 cut(s) 1114, 1541
MspR9I CCNGG 4 cut(s) 807, 1542, 1878, 1983
MunI CAATTG 3 cut(s) 606, 815, 1991
MvaI CCWGG 3 cut(s) 807, 1878, 1983
MvnI CGCG 2 cut(s) 1018, 2530
MwoI GCNNNNNNNGC 8 cut(s) 1052, 1213, 1242, 1760, 1845, 1857, 2156, 2363
NciI CCSGG 1 cut(s) 1542
NdeI CATATG 3 cut(s) 1241, 1429, 1894
NheI GCTAGC 1 cut(s) 2159
NlaIII CATG 6 cut(s) 1061, 1177, 1479, 1514, 1860, 2120
NlaIV GGNNCC 4 cut(s) 1118, 1450, 1988, 2199
NmuCI GTSAC 5 cut(s) 352, 553, 1604, 1781, 1944
NsbI TGCGCA 1 cut(s) 1401
NsiI ATGCAT 1 cut(s) 1957
NspI RCATGY 2 cut(s) 1860, 2120
PaeI GCATGC 1 cut(s) 1860
PcsI WCGNNNNNNNCGW 3 cut(s) 278, 532, 1197
PdmI GAANNNNTTC 1 cut(s) 2331
PflMI CCANNNNNTGG 2 cut(s) 1433, 2072
PkrI GCNGC 5 cut(s) 311, 1048, 1601, 1850, 1873
PleI GAGTC 1 cut(s) 1778
PpsI GAGTC 1 cut(s) 1778
PpuMI RGGWCCY 1 cut(s) 2197
PshBI ATTAAT 1 cut(s) 851
PsiI TTATAA 1 cut(s) 1589
Psp124BI GAGCTC 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 2197
Psp6I CCWGG 3 cut(s) 805, 1876, 1981
PspFI CCCAGC 2 cut(s) 93, 97
PspGI CCWGG 3 cut(s) 805, 1876, 1981
PspN4I GGNNCC 4 cut(s) 1118, 1450, 1988, 2199
PspPI GGNCC 2 cut(s) 2197, 2489
PspPPI RGGWCCY 1 cut(s) 2197
PstI CTGCAG 4 cut(s) 314, 580, 1873, 2368
PstNI CAGNNNCTG 1 cut(s) 1877
PsuI RGATCY 8 cut(s) 127, 421, 541, 565, 670, 1421, 2011, 2441
PvuII CAGCTG 1 cut(s) 97
RsaI GTAC 4 cut(s) 262, 781, 1969, 2002
RsaNI GTAC 4 cut(s) 261, 780, 1968, 2001
RseI CAYNNNNRTG 5 cut(s) 1304, 1542, 1668, 1940, 2145
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 5 cut(s) 310, 1047, 1600, 1849, 1872
Sau96I GGNCC 2 cut(s) 2197, 2489
SchI GAGTC 1 cut(s) 1778
ScrFI CCNGG 4 cut(s) 807, 1542, 1878, 1983
SduI GDGCHC 3 cut(s) 78, 1121, 1171
SfaNI GCATC 7 cut(s) 158, 243, 997, 1299, 1305, 2011, 2338
SfcI CTRYAG 4 cut(s) 310, 576, 1869, 2364
SinI GGWCC 2 cut(s) 2197, 2489
SmiMI CAYNNNNRTG 5 cut(s) 1304, 1542, 1668, 1940, 2145
SmlI CTYRAG 2 cut(s) 77, 1247
SmoI CTYRAG 2 cut(s) 77, 1247
SpeI ACTAGT 1 cut(s) 2291
SphI GCATGC 1 cut(s) 1860
SsiI CCGC 3 cut(s) 1018, 1677, 2528
SspI AATATT 1 cut(s) 601
SspMI CTAG 6 cut(s) 327, 569, 1221, 2102, 2160, 2292
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 4 cut(s) 805, 1540, 1876, 1981
StyI CCWWGG 1 cut(s) 2314
TaaI ACNGT 8 cut(s) 267, 414, 515, 559, 1283, 1801, 2057, 2517
TaiI ACGT 2 cut(s) 363, 2346
TaqI TCGA 5 cut(s) 64, 1135, 1191, 1575, 2450
TatI WGTACW 1 cut(s) 260
TscAI CASTG 9 cut(s) 319, 419, 585, 1528, 1609, 1663, 1786, 1858, 1942
TseFI GTSAC 5 cut(s) 352, 553, 1604, 1781, 1944
TseI GCWGC 5 cut(s) 309, 1046, 1599, 1848, 1871
Tsp45I GTSAC 5 cut(s) 352, 553, 1604, 1781, 1944
TspGWI ACGGA 3 cut(s) 287, 1093, 2441
TspRI CASTG 9 cut(s) 319, 419, 585, 1528, 1609, 1663, 1786, 1858, 1942
Van91I CCANNNNNTGG 2 cut(s) 1433, 2072
VpaK11BI GGWCC 2 cut(s) 2197, 2489
VspI ATTAAT 1 cut(s) 851
XapI RAATTY 4 cut(s) 343, 643, 1314, 1562
XbaI TCTAGA 2 cut(s) 326, 2101
XceI RCATGY 2 cut(s) 1860, 2120
XcmI CCANNNNNNNNNTGG 2 cut(s) 1359, 2152
XmiI GTMKAC 1 cut(s) 712
XmnI GAANNNNTTC 1 cut(s) 2331
XspI CTAG 6 cut(s) 327, 569, 1221, 2102, 2160, 2292
Zsp2I ATGCAT 1 cut(s) 1957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.